video microscopy

Summary

Summary: Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.

Top Publications

  1. pmc Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis
    E Bi
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    J Cell Biol 142:1301-12. 1998
  2. ncbi Ontogeny and behaviour of early macrophages in the zebrafish embryo
    P Herbomel
    Unité de Biologie moléculaire du Développement, URA1947 du CNRS, Departement de Biologie Moleculaire, Institut Pasteur, 75724 Paris Cedex 15, France
    Development 126:3735-45. 1999
  3. ncbi Centrosome-dependent exit of cytokinesis in animal cells
    M Piel
    Institut Curie, Section Recherche, UMR 144 du CNRS, 26 rue d'Ulm, 75248 Paris Cedex 05, France
    Science 291:1550-3. 2001
  4. ncbi In vivo imaging of leukocyte trafficking in blood vessels and tissues
    Thorsten R Mempel
    The CBR Institute for Biomedical Research and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Opin Immunol 16:406-17. 2004
  5. pmc Roles for 147 embryonic lethal genes on C.elegans chromosome I identified by RNA interference and video microscopy
    P Zipperlen
    University of Cambridge, Wellcome CRC Institute and Department of Genetics, Tennis Court Road, Cambridge CB2 1QR, UK
    EMBO J 20:3984-92. 2001
  6. ncbi Direct observation of epicardial coronary capillary hemodynamics during reactive hyperemia and during adenosine administration by intravital video microscopy
    Takahiko Kiyooka
    Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine and Dentistry, Japan
    Am J Physiol Heart Circ Physiol 288:H1437-43. 2005
  7. pmc Circulating blood cells function as a surveillance system for damaged tissue in Drosophila larvae
    Daniel T Babcock
    Department of Biochemistry and Molecular Biology, University of Texas Graduate School of Biomedical Sciences, University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:10017-22. 2008
  8. ncbi Motility and ramification of human fetal microglia in culture: an investigation using time-lapse video microscopy and image analysis
    P Rezaie
    Department of Neuropathology, Institute of Psychiatry, King s College London, DeCrespigny Park, London, SE5 8AF, United Kingdom
    Exp Cell Res 274:68-82. 2002
  9. pmc Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis
    Wulf Haubensak
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 101:3196-201. 2004
  10. pmc Quantitative 3D video microscopy of HIV transfer across T cell virological synapses
    Wolfgang Hubner
    Division of Infectious Diseases, Department of Medicine, Immunology Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    Science 323:1743-7. 2009

Research Grants

Detail Information

Publications307 found, 100 shown here

  1. pmc Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis
    E Bi
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    J Cell Biol 142:1301-12. 1998
    ..cerevisiae. In its absence, cytokinesis can still be completed by a process (possibly localized cell-wall synthesis leading to septum formation) that appears to require septin function and to be facilitated by F-actin...
  2. ncbi Ontogeny and behaviour of early macrophages in the zebrafish embryo
    P Herbomel
    Unité de Biologie moléculaire du Développement, URA1947 du CNRS, Departement de Biologie Moleculaire, Institut Pasteur, 75724 Paris Cedex 15, France
    Development 126:3735-45. 1999
    ....
  3. ncbi Centrosome-dependent exit of cytokinesis in animal cells
    M Piel
    Institut Curie, Section Recherche, UMR 144 du CNRS, 26 rue d'Ulm, 75248 Paris Cedex 05, France
    Science 291:1550-3. 2001
    ....
  4. ncbi In vivo imaging of leukocyte trafficking in blood vessels and tissues
    Thorsten R Mempel
    The CBR Institute for Biomedical Research and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Opin Immunol 16:406-17. 2004
    ....
  5. pmc Roles for 147 embryonic lethal genes on C.elegans chromosome I identified by RNA interference and video microscopy
    P Zipperlen
    University of Cambridge, Wellcome CRC Institute and Department of Genetics, Tennis Court Road, Cambridge CB2 1QR, UK
    EMBO J 20:3984-92. 2001
    ....
  6. ncbi Direct observation of epicardial coronary capillary hemodynamics during reactive hyperemia and during adenosine administration by intravital video microscopy
    Takahiko Kiyooka
    Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine and Dentistry, Japan
    Am J Physiol Heart Circ Physiol 288:H1437-43. 2005
    Using high-resolution intravital charge-coupled device video microscopy, we visualized the epicardial capillary network of the beating canine heart in vivo to elucidate its functional role under control conditions, during reactive ..
  7. pmc Circulating blood cells function as a surveillance system for damaged tissue in Drosophila larvae
    Daniel T Babcock
    Department of Biochemistry and Molecular Biology, University of Texas Graduate School of Biomedical Sciences, University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:10017-22. 2008
    ....
  8. ncbi Motility and ramification of human fetal microglia in culture: an investigation using time-lapse video microscopy and image analysis
    P Rezaie
    Department of Neuropathology, Institute of Psychiatry, King s College London, DeCrespigny Park, London, SE5 8AF, United Kingdom
    Exp Cell Res 274:68-82. 2002
    ..Analysis of progressive cultures using time-lapse video microscopy clearly demonstrates the dynamic nature of microglia...
  9. pmc Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis
    Wulf Haubensak
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Proc Natl Acad Sci U S A 101:3196-201. 2004
    ..Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex...
  10. pmc Quantitative 3D video microscopy of HIV transfer across T cell virological synapses
    Wolfgang Hubner
    Division of Infectious Diseases, Department of Medicine, Immunology Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    Science 323:1743-7. 2009
    ..Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein...
  11. ncbi Single-particle tracking of oriC-GFP fluorescent spots during chromosome segregation in Escherichia coli
    Steven Elmore
    Section Molecular Cytology, Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands
    J Struct Biol 151:275-87. 2005
    ..We conclude from the analysis that within the experimental accuracy--the limits of which are indicated and discussed--there is no evidence that spot segregation requires any other mechanism than that of cell (length) growth...
  12. pmc Measurement of functional microcirculatory geometry and velocity distributions using automated image analysis
    J G G Dobbe
    Department of Physiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    Med Biol Eng Comput 46:659-70. 2008
    ..RBC velocity was reduced by 70% during the first 10 s of cardiac luxation. The present method has been shown to be fast and accurate and provides increased insight into the functional properties of the microcirculation...
  13. pmc Role of phosphatidylinositol 3' kinase and a downstream pleckstrin homology domain-containing protein in controlling chemotaxis in dictyostelium
    S Funamoto
    Section of Cell and Developmental Biology and Center for Molecular Genetics, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    J Cell Biol 153:795-810. 2001
    ..We suggest that PKB and PhdA lie downstream from PI3K and control different downstream effector pathways that are essential for proper chemotaxis...
  14. pmc Modulation of cell migration by integrin-mediated cytoskeletal linkages and ligand-binding affinity
    A Huttenlocher
    Department of Cell and Structural Biology, University of Illinois, Urbana 61801, USA
    J Cell Biol 134:1551-62. 1996
    ..Further, we demonstrate a dominant effect of high affinity integrin in inhibiting migration regardless of the organization of adhesive complexes. These observations have potential implications for tumor metastasis and its therapy...
  15. ncbi Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy
    Graham M Gibson
    Department of Physics and Astronomy, University of Glasgow, Glasgow, UK
    Opt Express 16:14561-70. 2008
    ..As an example of a multi-particle measurement we show the hydrodynamic coupling between two particles...
  16. ncbi Propagating chemoattractant waves coordinate periodic cell movement in Dictyostelium slugs
    D Dormann
    School of Life Sciences, Division of Cell and Developmental Biology, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    Development 128:4535-43. 2001
    ..Isolated tips continue to migrate while producing waves. We conclude from these observations that the tip acts as a pacemaker for cAMP waves that coordinate cell movement in slugs...
  17. ncbi The orientation of cell division influences cell-fate choice in the developing mammalian retina
    Michel Cayouette
    MRC Laboratory for Molecular Cell Biology and Cell Biology Unit, University College London, London WC1E 6BT, UK
    Development 130:2329-39. 2003
    ..These findings indicate that the plane of cell division influences cell-fate choice in the neonatal rat retina and support the hypothesis that the asymmetric segregation of Numb normally influences some of these choices...
  18. ncbi Strategies for three-dimensional particle tracking with holographic video microscopy
    Fook Chiong Cheong
    Department of Physics and Center for Soft Matter Research, New York University, New York, NY 10003, USA
    Opt Express 18:13563-73. 2010
    ..The two techniques agree quantitatively on colloidal spheres' in-plane positions. Their systematic differences in axial tracking can be explained in terms of the illuminated objects' light scattering properties...
  19. pmc Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells
    Pirta Hotulainen
    Program in Cellular Biotechnology, Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland
    Mol Biol Cell 16:649-64. 2005
    ..These data suggest that mammalian ADF and cofilin-1 promote cytoskeletal dynamics by depolymerizing actin filaments and that this activity is critical for several processes such as cytokinesis and cell motility...
  20. pmc Separation of propulsive and adhesive traction stresses in locomoting keratocytes
    T Oliver
    Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708 0300, USA
    J Cell Biol 145:589-604. 1999
    ....
  21. pmc Significantly improved precision of cell migration analysis in time-lapse video microscopy through use of a fully automated tracking system
    Johannes Huth
    Research group of Bioinformatics and Systems Biology, Institute of Neural Information Processing, Ulm University, Albert Einstein Allee 11, D 89081 Ulm, Germany
    BMC Cell Biol 11:24. 2010
    ..In time-lapse video microscopy, manual cell tracking remains the most common method of analyzing migratory behavior of cell populations...
  22. ncbi Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules
    Akihiro Kusumi
    Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology Organization, Department of Biological Science and Institute for Advanced Research, Nagoya University, Nagoya 464 8602, Japan
    Annu Rev Biophys Biomol Struct 34:351-78. 2005
    ....
  23. pmc The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors
    S L Reck-Peterson
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 153:1121-6. 2001
    ..These studies suggest that if Myo2p functions in organelle transport, at least five molecules of Myo2p must be present per organelle to promote directed movement...
  24. ncbi Clinical targets for anti-metastasis therapy
    A F Chambers
    Department of Oncology, University of Western Ontario, Canada
    Adv Cancer Res 79:91-121. 2000
    ..A variety of therapeutic approaches to target this phase are under active development, including inhibition of angiogenesis or signal transduction pathways needed to support the growth of metastatic cells...
  25. pmc The respective contributions of the mother and daughter centrioles to centrosome activity and behavior in vertebrate cells
    M Piel
    Institut Curie, Section Recherche, UMR 144 du CNRS, 75248 Paris Cedex 05, France
    J Cell Biol 149:317-30. 2000
    ..during G1 and S only the mother centriole is located at the focus of the Mt array. A model, based on differences in Mt anchoring and release by the mother and daughter centrioles, is proposed to explain these results...
  26. pmc Importance of integrin LFA-1 deactivation for the generation of immune responses
    Monika Semmrich
    Department of Surgery, Immunology, and Hygiene, Technische Universitat Munchen, 81675 Munich, Germany
    J Exp Med 201:1987-98. 2005
    ..Thus, deactivation of LFA-1 and disassembly of LFA-1-mediated cell contacts seem to be vital for the generation of normal immune responses...
  27. pmc Integrin and cadherin synergy regulates contact inhibition of migration and motile activity
    A Huttenlocher
    Department of Cell and Structural Biology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Cell Biol 141:515-26. 1998
    ..These observations have potential importance to normal and pathologic processes during embryonic development and tumor metastasis...
  28. ncbi Intravital imaging of cell movement in tumours
    John Condeelis
    Department of Anatomy and Structural Biology, Program in Motility and Invasion, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nat Rev Cancer 3:921-30. 2003
  29. ncbi Intravital microscopy: visualizing immunity in context
    Cenk Sumen
    The CBR Institute for Biomedical Research, Boston, MA 02115, USA
    Immunity 21:315-29. 2004
    ..This article will discuss the current state of the field and outline how IVM can generate new discoveries and serve as a "reality check" for areas of research that were formerly the exclusive domain of in vitro experimentation...
  30. ncbi Technique for using video microscopy and indicator dilution for repeated measurements of cardiac output in small animals
    R J Rivers
    Department of Anesthesiology, University of Rochester School of Medicine and Dentistry, New York 14642, USA
    Anesthesiology 94:489-95. 2001
    ..2 +/- 0.5 ml/100 g body weight, within the normal range for hamsters. CONCLUSIONS: Fluorescent dye dilution and video microscopy can be used to repeatedly determine cardiac output or blood volume in small animals.
  31. pmc Retrograde axonal transport of herpes simplex virus: evidence for a single mechanism and a role for tegument
    E L Bearer
    Department of Pathology and Laboratory Medicine, Brown University, Providence, RI 02912, USA
    Proc Natl Acad Sci U S A 97:8146-50. 2000
    ....
  32. ncbi Clostridial gas gangrene. II. Phospholipase C-induced activation of platelet gpIIbIIIa mediates vascular occlusion and myonecrosis in Clostridium perfringens gas gangrene
    A E Bryant
    VA Medical Center, Infectious Diseases Section, Boise, ID 83702, USA
    J Infect Dis 182:808-15. 2000
    ..Therapeutic strategies that target gpIIbIIIa may prevent vascular occlusion, maintain tissue viability, and provide an alternative to radical amputation for patients with this infection...
  33. ncbi Visualization of fast-moving cells in vivo using digital holographic video microscopy
    Hongyue Sun
    University of Aberdeen, School of Engineering, Aberdeen AB24 3UE, Scotland, United Kingdom
    J Biomed Opt 13:014007. 2008
    ..By combining holography with digital video microscopy, an in-line holographic video microscope is developed and is capable of recording spatial 3D holographic ..
  34. ncbi Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER
    L Pelkmans
    Institute of Biochemistry, Swiss Federal Institute of Technology, Universitaetstrasse 16, , Switzerland
    Nat Cell Biol 3:473-83. 2001
    ..This pathway bypasses endosomes and the Golgi complex, and is part of the productive infectious route used by SV40...
  35. pmc The multiple roles of Cyk1p in the assembly and function of the actomyosin ring in budding yeast
    K B Shannon
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 10:283-96. 1999
    ..In this study, video microscopy analysis of cells expressing green fluorescent protein-tagged Cyk1p/Iqg1p demonstrates that Cyk1p/Iqg1p is a ..
  36. ncbi Flow visualization and flow cytometry with holographic video microscopy
    Fook Chiong Cheong
    Department of Physics and Center for Soft Matter Research, New York University, New York, NY 10003, USA
    Opt Express 17:13071-9. 2009
    ..We demonstrate this approach to label-free holographic flow cytometry by detecting the binding of avidin to biotinylated polystyrene spheres...
  37. ncbi Rotational and translational diffusion of copper oxide nanorods measured with holographic video microscopy
    Fook Chiong Cheong
    Department of Physics and Center for Soft Matter Research, New York University, New York, NY 10003, USA
    Opt Express 18:6555-62. 2010
    We use holographic video microscopy to track the three-imensional translational and rotational diffusion of copper oxide nanorods suspended in water...
  38. pmc Neutrophils lacking phosphoinositide 3-kinase gamma show loss of directionality during N-formyl-Met-Leu-Phe-induced chemotaxis
    Michael Hannigan
    Department of Pathology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
    Proc Natl Acad Sci U S A 99:3603-8. 2002
    ..These data suggest that PI3Kgamma regulates neutrophil chemotaxis primarily by controlling the direction of cell migration and the intracellular colocalization of AKT and F-actin to the leading edge...
  39. pmc Asymmetrical flagellar rotation in Borrelia burgdorferi nonchemotactic mutants
    Chunhao Li
    Department of Microbiology, Immunology, and Cell Biology, Health Sciences Center, Box 9177, West Virginia University, Morgantown, WV 26506 9177, USA
    Proc Natl Acad Sci U S A 99:6169-74. 2002
    ..Rather than flexing, it failed to reverse, and it continually translated only in one direction. Video microscopy of mutant cells indicated that both bundles actively rotated...
  40. ncbi Continuous real time ex vivo epifluorescent video microscopy for the study of metastatic cancer cell interactions with microvascular endothelium
    Olga V Glinskii
    Department of Biochemistry, University of Missouri, Columbia, Missouri 65212, USA
    Clin Exp Metastasis 20:451-8. 2003
    ....
  41. pmc Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila
    D P Kiehart
    Department of Cell Biology, Cell and Molecular Biology Program, University Program in Genetics and the Duke Comprehensive Cancer Center, Duke University Medical Center, Durham, North Carolina 27710 3709, USA
    J Cell Biol 149:471-90. 2000
    ..Moreover, our observations demonstrate that wound healing in this insect epidermal system parallel wound healing in vertebrate tissues in situ and vertebrate cells in culture (for review see Kiehart, D.P. 1999. Curr. Biol. 9:R602-R605)...
  42. ncbi Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy
    Xiaowei Chen
    HCNR Center for Bioinformatics, Harvard Medical School, Boston, MA 02115, USA
    IEEE Trans Biomed Eng 53:762-6. 2006
    ..Experimental results show that the proposed method is efficient and effective in cell tracking and phase identification...
  43. ncbi Chronology of cellular alterations during 7-ketocholesterol-induced cell death on A7R5 rat smooth muscle cells: analysis by time lapse-video microscopy and conventional fluorescence microscopy
    Jean Marie Zahm
    Institut National de la Santé et de la Recherche Médicale U514, Reims, France
    Cytometry A 52:57-69. 2003
    Time-lapse video microscopy was used to determine whether mitochondrial and nuclear changes (decrease in mitochondrial transmembrane potential, condensation, and/or fragmentation of the nuclei, morphologic features typical of apoptosis) ..
  44. pmc Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells
    Wendy C Salmon
    Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 158:31-7. 2002
    ..These results show that the movement and organization of f-actin has a profound effect on the dynamic organization of MTs in migrating cells, and suggest that MTs and f-actin bind to one another in vivo...
  45. ncbi Measuring microlymphatic flow using fast video microscopy
    J Brandon Dixon
    Texas A and M University, Department of Biomedical Engineering, Mail Stop 3120, College Station, Texas 77843, USA
    J Biomed Opt 10:064016. 2005
    ..In this work, video microscopy and particle tracking methods are adapted and integrated with an ultra-high-speed imaging camera to obtain ..
  46. ncbi Phototriggering system for an ultrahigh-speed video microscopy
    Pavel Karimov
    School of Science and Engineering, Kinki University, Higashi Osaka 577 8502, Japan
    Rev Sci Instrum 78:113702. 2007
    ..This is sufficiently fast for high-speed video-microscopy observations at 1x10(6) frames/s when using a video camera with a storage of 103 frames. As an example, the system was used in a microscopic observation of a soap film collapse...
  47. pmc An imaging system for standardized quantitative analysis of C. elegans behavior
    Zhaoyang Feng
    Division of Biological Sciences, University of California, San Diego, CA USA
    BMC Bioinformatics 5:115. 2004
    ..Because traditional methods for evaluating behavioral phenotypes are qualitative and imprecise, there is a need for tools that allow quantitation and standardization of C. elegans behavioral assays...
  48. pmc Nuclear size control in fission yeast
    Frank R Neumann
    The Rockefeller University, New York, NY 10065, USA
    J Cell Biol 179:593-600. 2007
    ..We propose that there is a general cellular control linking nuclear growth to cell size...
  49. ncbi Xkid, a chromokinesin required for chromosome alignment on the metaphase plate
    C Antonio
    Cell Biology and Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 102:425-35. 2000
    ..Our results show that these forces are essential to ensure that kinetochores and chromosome arms align on a narrow equatorial plate during metaphase, a prerequisite for proper chromosome segregation...
  50. ncbi Persistent microcirculatory alterations are associated with organ failure and death in patients with septic shock
    Yasser Sakr
    Department of Intensive Care, Erasme Hospital, Free University of Brussels, Belgium
    Crit Care Med 32:1825-31. 2004
    ..To characterize the time course of microcirculatory alterations and their relation to outcome in patients with septic shock...
  51. pmc An automated system for measuring parameters of nematode sinusoidal movement
    Christopher J Cronin
    HHMI and Division of Biology, California Institute of Technology, Pasadena, CA, USA
    BMC Genet 6:5. 2005
    ..An enhanced capability to analyze all the complexities of nematode movement will thus help our understanding of how genes control behavior...
  52. pmc Super-resolution video microscopy of live cells by structured illumination
    Peter Kner
    Department of Biochemistry and Biophysics, San Francisco, California, USA
    Nat Methods 6:339-42. 2009
    ..We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode...
  53. pmc Polarized insertion of new membrane from a cytoplasmic reservoir during cleavage of the Drosophila embryo
    T Lecuit
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Cell Biol 150:849-60. 2000
    ..We thus identify a tightly regulated scheme of polarized membrane insertion during cellularization. We propose that such a mechanism could participate in the progressive emergence of apical-basal polarity...
  54. pmc Entry into mitosis in vertebrate somatic cells is guarded by a chromosome damage checkpoint that reverses the cell cycle when triggered during early but not late prophase
    C L Rieder
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York 12201 0509, USA
    J Cell Biol 142:1013-22. 1998
    ..Together, these data reveal that a checkpoint control exists in early but not late prophase in vertebrate cells that, when triggered, reverses the cell cycle by apparently downregulating existing cyclin-dependent kinase (CDK1) activity...
  55. ncbi Role of the actin bundling protein fascin in growth cone morphogenesis: localization in filopodia and lamellipodia
    C S Cohan
    Department of Anatomy and Cell Biology, University at Buffalo, SUNY, Buffalo, New York 14214, USA
    Cell Motil Cytoskeleton 48:109-20. 2001
    ..Our findings suggest that fascin may be particularly important for the linear structure and dynamics of filopodia and for lamellipodial rib dynamics by regulating filament organization in bundles...
  56. ncbi In vivo time-lapse imaging of synaptic takeover associated with naturally occurring synapse elimination
    Mark K Walsh
    Department of Anatomy and Neurobiology, Washington University School of Medicine, Saint Louis, MO 63110, USA
    Neuron 37:67-73. 2003
    ..These results show synaptic competition is not always monotonic and that one axon's contraction in synaptic area is associated with another axon's expansion...
  57. ncbi Visualization of leukocyte transendothelial and interstitial migration using reflected light oblique transillumination in intravital video microscopy
    Thorsten R Mempel
    Institute for Surgical Research, University of Munich, Munich, Germany
    J Vasc Res 40:435-41. 2003
    ....
  58. pmc Dual regulation of actin rearrangement through lysophosphatidic acid receptor in neuroblast cell lines: actin depolymerization by Ca(2+)-alpha-actinin and polymerization by rho
    Nobuyuki Fukushima
    Department of Molecular Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo 060 8638, Japan
    Mol Biol Cell 13:2692-705. 2002
    ..This dual actin rearrangement may play a novel, important role in regulation of the neuronal morphology and motility during brain development...
  59. ncbi 3D culture model and computer-assisted videomicroscopy to analyze migratory behavior of noninvasive and invasive bronchial epithelial cells
    Salma Hazgui
    INSERM UMRS 514, 45 rue Cognacq Jay, 51092 Reims, France
    Am J Physiol Cell Physiol 289:C1547-52. 2005
    ....
  60. ncbi Phosphorylation of ezrin on threonine T567 plays a crucial role during compaction in the mouse early embryo
    Nicolas Dard
    Laboratoire de Biologie Cellulaire du Developpement, UMR7622, CNRS Universite Pierre et Marie Curie, 9 Quai St Bernard, 75252 Paris Cedex 05, France
    Dev Biol 271:87-97. 2004
    ....
  61. ncbi Dynamics of alpha-actinin in focal adhesions and stress fibers visualized with alpha-actinin-green fluorescent protein
    M Edlund
    Department of Cell Biology, University of Virginia, Charlottesville, USA
    Cell Motil Cytoskeleton 48:190-200. 2001
    ..Additionally, the exchange rates revealed by video-microscopy and FRAP analysis of alpha AGFP are more rapid than those reported previously, which were obtained using microinjection of large excesses of fluorescently-tagged protein...
  62. ncbi Microtubules and mitotic cycle phase modulate spatiotemporal distributions of F-actin and myosin II in Drosophila syncytial blastoderm embryos
    V E Foe
    Department of Zoology, University of Washington, Seattle WA 98195 1800, USA
    Development 127:1767-87. 2000
    ....
  63. ncbi Tracking of migrating cells under phase-contrast video microscopy with combined mean-shift processes
    O Debeir
    Department of Logical and Numerical Systems, Faculty of Applied Sciences, CP 165 57, Universite Libre de Bruxelles, 50 Av F Roosevelt, 1050 Brussels, Belgium
    IEEE Trans Med Imaging 24:697-711. 2005
    ..mean-shift-based tracking processes to establish migrating cell trajectories through in vitro phase-contrast video microscopy. After a recapitulation on how the mean-shift algorithm permits efficient object tracking we describe the ..
  64. pmc The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport
    Joshua Mueller
    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Biol Cell 16:1341-54. 2005
    ..KAP is therefore required for the localization of Kinesin-2 at the site of flagellar assembly and the efficient transport of anterograde IFT particles within flagella...
  65. ncbi Plastid stromules: video microscopy of their outgrowth, retraction, tensioning, anchoring, branching, bridging, and tip-shedding
    Brian E S Gunning
    Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, Canberra, A C T, Australia
    Protoplasma 225:33-42. 2005
    ..Formation and movement of stromular branches and bridges between plastids are described. The shedding of a tip region into the streaming cytoplasm is recorded in frame-by-frame detail, in accord with early observations...
  66. ncbi The L3 to L4 molt of Brugia malayi: real time visualization by video microscopy
    Manish Ramesh
    Department of Pathology, University of Connecticut Health Center, Farmington 06030 3105, USA
    J Parasitol 91:1028-33. 2005
    ..This has permitted us to visualize, for the first time, details of this molt using real-time video microscopy. Molting is preceded by a phase of altered motility during which the larva exhibits contractile, coiling ..
  67. pmc Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host
    Tara J Moriarty
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    PLoS Pathog 4:e1000090. 2008
    ..To our knowledge, this is the first report of high resolution 3D and 4D visualization of dissemination of a bacterial pathogen in a living mammalian host, and provides the first direct insight into spirochete dissemination in vivo...
  68. ncbi Deterministic and probabilistic approaches for tracking virus particles in time-lapse fluorescence microscopy image sequences
    W J Godinez
    Department of Bioinformatics and Functional Genomics, Biomedical Computer Vision Group, University of Heidelberg, BIOQUANT, IPMB, and DKFZ Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany
    Med Image Anal 13:325-42. 2009
    ..It turns out that the probabilistic approaches based on independent particle filters are superior to the deterministic schemes as well as to the approaches based on a mixture of particle filters...
  69. ncbi Capillary flow and diameter changes during reperfusion after global cerebral ischemia studied by intravital video microscopy
    Erik F Hauck
    Divison of Neurosurgery, University of Texas Medical Branch, Galveston, USA
    J Cereb Blood Flow Metab 24:383-91. 2004
    ....
  70. ncbi Phagosome formation in Paramecium: roles of somatic and oral cilia and of solid particles as revealed by video microscopy
    M Ishida
    Department of Physiology, Mie University School of Medicine, Tsu, Japan
    J Eukaryot Microbiol 48:640-6. 2001
    ..The beating of the peniculi may somehow prevent the backward flow of particles out of the NDV...
  71. pmc Time-lapse video microscopy analysis reveals astral microtubule detachment in the yeast spindle pole mutant cnm67
    D Hoepfner
    Abteilung Molekulare Mikrobiologie, Biozentrum, Universitat Basel, CH 4056 Basel, Switzerland
    Mol Biol Cell 11:1197-211. 2000
    ..Interestingly, the first nuclear segregation in newborn diploid cells never fails, even though astral microtubule detachment occurs...
  72. pmc Action potential propagation imaged with high temporal resolution near-infrared video microscopy and polarized light
    Jennifer L Schei
    Department of Physics and Astronomy, Washington State University, Pullman, WA 99164 2814, USA
    Neuroimage 40:1034-43. 2008
    ..We tested the effect of randomizing cell orientation by tying the nerve in an overhand knot prior to imaging, producing polarization changes that can be imaged even without regular cell orientations...
  73. ncbi Identification and quantification of skeletal myotube contraction and association in vitro by video microscopy
    Christopher G Langhammer
    Department of Biomedical Engineering, Rutgers University, Busch Campus, Piscataway, New Jersey 08854, USA
    Cytoskeleton (Hoboken) 67:413-24. 2010
    ..b>Video microscopy provides a noninvasive way to record the contractile behavior of skeletal muscle cells in vitro...
  74. ncbi Origins and unconventional behavior of neutrophils in developing zebrafish
    Dorothée Le Guyader
    Unité Macrophages et Développement de l Immunité, Centre National de la Recherche Scientifique Unité de Recherche Associée 2578, Institut Pasteur, Paris, France
    Blood 111:132-41. 2008
    ..They are also attracted to variously stressed or malformed tissues, suggesting a wider role than antimicrobial defense...
  75. ncbi Automatic tracking, feature extraction and classification of C elegans phenotypes
    Wei Geng
    Department of Electrical and Computer Engineering, University of California at San Diego, La Jolla, CA 92093 0407, USA
    IEEE Trans Biomed Eng 51:1811-20. 2004
    ..9%. The features that have most discrimination ability were also studied. The algorithm developed will be an essential part of a completely automated C. elegans tracking and identification system...
  76. ncbi Allicin inhibits cell polarization, migration and division via its direct effect on microtubules
    Masha Prager-Khoutorsky
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel
    Cell Motil Cytoskeleton 64:321-37. 2007
    ..Thus, allicin is a potent microtubule-disrupting reagent interfering with tubulin polymerization by reaction with tubulin SH groups...
  77. pmc Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism
    Sophie Mary
    Centre de Recherche de Biochimie Macromoleculaire, Centre National de la Recherche Scientifique Unité Propre de Recherche 1086, 34293 Montpellier, France
    Mol Biol Cell 13:285-301. 2002
    ..In conclusion, we propose that N-cadherin distribution is a regulated process promoted by cell-cell contact formation, which controls the biogenesis and turnover of the junctions through the MT network...
  78. ncbi LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment
    Andrew Smith
    Leukocyte Adhesion Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, Lincoln s Inn Fields, London WC2A 3PX, UK
    J Cell Sci 116:3123-33. 2003
    ..These two actomyosin-dependent processes operate coordinately to cause forward migration of a T cell...
  79. ncbi L-selectin facilitates emigration and extravascular locomotion of leukocytes during acute inflammatory responses in vivo
    M J Hickey
    Immunology Research Group, University of Calgary, Calgary, Alberta, Canada
    J Immunol 165:7164-70. 2000
    ..These findings indicate that L-selectin is important in enabling leukocytes to respond effectively to chemotactic stimuli in inflamed tissues...
  80. ncbi A new video image analysis system to study red blood cell dynamics and oxygenation in capillary networks
    Shruti A Japee
    Department of Biomedical Engineering, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, 23298, USA
    Microcirculation 12:489-506. 2005
    ....
  81. pmc Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling
    C M Waterman-Storer
    Department of Biology, 607 Fordham Hall, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    J Cell Biol 139:417-34. 1997
    ..We propose that actomyosin-based retrograde flow of MTs causes MT breakage, forming quasi-stable noncentrosomal MTs whose turnover is regulated primarily at their minus ends...
  82. pmc Distinct mutants of retrograde intraflagellar transport (IFT) share similar morphological and molecular defects
    G Piperno
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, 10029, USA
    J Cell Biol 143:1591-601. 1998
    ..The occurrence of the same set of phenotypes, as the result of a mutation in any one of three loci, suggests the hypothesis that complex A is a portion of the IFT particles specifically involved in retrograde intraflagellar movement...
  83. ncbi A new protocol for functional analysis of adipogenesis using reverse transfection technology and time-lapse video microscopy
    Elke Grönniger
    Research and Development, Research Special Skincare, Beiersdorf AG, Unnastrasse 48, 20245 Hamburg, Germany
    Cell Biol Int 34:737-46. 2010
    ..In conclusion, we established a protocol that allows for an efficient functional and time-dependent analysis by quantitative time-lapse microscopy to identify novel adipogenesis-associated genes...
  84. ncbi A novel model for the investigation of orthotopically growing primary and secondary bone tumours using intravital microscopy
    F M Klenke
    Department of Orthopaedic Surgery, University of Heidelberg, Schlierbacher Landstrasse 200a, D 69118 Heidelberg, Germany
    Lab Anim 39:377-83. 2005
    ....
  85. ncbi Costimulation and endogenous MHC ligands contribute to T cell recognition
    Christoph Wulfing
    The Howard Hughes Medical Institute and The Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Nat Immunol 3:42-7. 2002
    ..Thus, low-affinity ligands can contribute to synapse formation and T cell signaling...
  86. ncbi Golgi biogenesis in Toxoplasma gondii
    Laurence Pelletier
    Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nature 418:548-52. 2002
    ..Our results indicate that new Golgi grow by autonomous duplication and raise the possibility that the Golgi is a paired structure that is analogous to centrioles...
  87. pmc The fastest flights in nature: high-speed spore discharge mechanisms among fungi
    Levi Yafetto
    Department of Botany, Miami University, Oxford, Ohio, USA
    PLoS ONE 3:e3237. 2008
    ..Because spores are discharged at such high speeds, most of the information on launch processes from previous studies has been inferred from mathematical models and is subject to a number of errors...
  88. pmc Development of factors to convert frequency to rate for beta-cell replication and apoptosis quantified by time-lapse video microscopy and immunohistochemistry
    Yoshifumi Saisho
    Larry Hillblom Islet Research Center, UCLA David Geffen School of Medicine, 900 Weyburn Place A, Los Angeles, CA 90024 2852, USA
    Am J Physiol Endocrinol Metab 296:E89-96. 2009
    ..by quantifying the relationship between the rate of beta-cell replication observed directly by time-lapse video microscopy (TLVM) and the frequency of beta-cell replication in the same islets detected by immunohistochemistry using ..
  89. ncbi Dendritic cell maturation triggers retrograde MHC class II transport from lysosomes to the plasma membrane
    Amy Chow
    Department of Cell Biology and Section of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, PO Box 208002, New Haven, Connecticut 06520 8002, USA
    Nature 418:988-94. 2002
    ..Here we have visualized this pathway in live dendritic cells by video microscopy, using cells expressing MHC II tagged with green fluorescent protein (GFP)...
  90. pmc Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not
    Cristiana Mollinari
    Institut de Biologie Structurale J P Ebel Commissariat à l Energie Atomique Centre National de la Recherche Scientifique Université Joseph Fournier, 38027 Grenoble Cedex 1, France
    Mol Biol Cell 16:1043-55. 2005
    ..Furrowing goes to completion, but abscission to create two daughter cells fails. We conclude the central spindle bundle is required for abscission but not for furrowing in mammalian cells...
  91. ncbi In vivo functional analysis of ezrin during mouse blastocyst formation
    N Dard
    , UMR 7622, , 9 quai Saint-Bernard, Paris Cedex 05, 75252, France
    Dev Biol 233:161-73. 2001
    ..Our results suggest that, in addition to its role in microvilli organization, ezrin is involved in the formation of a functional epithelium through a still unknown mechanism...
  92. ncbi Real-time visualization of mycobacterium-macrophage interactions leading to initiation of granuloma formation in zebrafish embryos
    J Muse Davis
    Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Immunity 17:693-702. 2002
    ..Strikingly, infection can redirect normal embryonic macrophage migration, even recruiting macrophages seemingly committed to their developmentally dictated tissue sites...
  93. ncbi Integrin alpha(2)beta(1) (VLA-2) is a principal receptor used by neutrophils for locomotion in extravascular tissue
    J Werr
    Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
    Blood 95:1804-9. 2000
    ..Blood. 2000;95:1804-1809)..
  94. ncbi Circadian modulation of calcium levels in cells in the suprachiasmatic nucleus
    C S Colwell
    Mental Retardation Research Center, Department of Psychiatry and Biobehavioural Sciences, University of California Los Angeles, 760 Westwood Plaza, Los Angeles, CA 90024 1759, USA
    Eur J Neurosci 12:571-6. 2000
    ..Because [Ca2+]i is known to play a critical role in many cellular processes, the presence of this rhythm is likely to have many implications for the cell biology of SCN neurons...
  95. ncbi Imaging early macrophage differentiation, migration, and behaviors in live zebrafish embryos
    Philippe Herbomel
    Unité Macrophages et Développement de l Immunité, Institut Pasteur, Paris, France
    Methods Mol Med 105:199-214. 2005
    ....
  96. pmc RhoA is required for monocyte tail retraction during transendothelial migration
    R A Worthylake
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 154:147-60. 2001
    ..Time-lapse video microscopy analysis revealed that C3-loaded monocytes also had significant forward crawling movement on the endothelial ..
  97. ncbi In vivo time-lapse imaging of cell divisions during neurogenesis in the developing zebrafish retina
    Tilak Das
    Department of Anatomy, University of Cambridge, Cambridge CB2 3DY, United Kingdom
    Neuron 37:597-609. 2003
    ..The reconstructions here show that mitotic cells always remain in contact with the opposite basal surface by means of a thin basal process that can be inherited asymmetrically...
  98. ncbi Requirement of Rac1 and Rac2 expression by mature dendritic cells for T cell priming
    Federica Benvenuti
    Unité INSERM 365, Institut Curie, 26 Rue d Ulm, 75005 Paris, France
    Science 305:1150-3. 2004
    ..We used time-lapse video microscopy and two-photon imaging of intact lymph nodes to show that after establishing initial contact between their ..
  99. pmc Single-particle tracking for DNA tether length monitoring
    Noëlle Pouget
    Institut de Pharmacologie et Biologie Structurale UMR CNRS 5089, 205 route de Narbonne, 31077 Toulouse Cedex, France
    Nucleic Acids Res 32:e73. 2004
    ..An advantage of this method is that the trajectory of the tethered bead, rather than its averaged motion, is measured, allowing analysis of the conformational dynamics of DNA chains at the single-molecule level...
  100. ncbi Off-line analysis of red blood cell velocity in renal arterioles
    A S De Vriese
    Renal Unit, Department of Internal Medicine, University Hospital, Gent, Belgium
    J Vasc Res 37:26-31. 2000
    ..The present approach extends the application of videomicroscopy coupled to image analysis systems to the analysis of high V(RBC)...
  101. ncbi Multiple particle tracking in 3-D+t microscopy: method and application to the tracking of endocytosed quantum dots
    Auguste Genovesio
    Unité d Analyse d Images Quantitative, CNRS URA 2582, Institut Pasteur, 75015 Paris, France
    IEEE Trans Image Process 15:1062-70. 2006
    ..The performances of the method are validated on synthetic image data and used to characterize the 3-D movement of endocytic vesicles containing quantum dots...

Research Grants62

  1. Ang II and Aldosterone Effects on Insulin Resistance in Cardiovascular Tissue
    JAMES RUSSELL SOWERS; Fiscal Year: 2013
    ..We will also employ direct visualization of skeletal muscle arterioles using video microscopy to evaluate insulin induced nitric oxide dependent vasodilation...
  2. Pilot Studies of the Effect of Aging on Mucociliary Clearance
    Lawrence E Ostrowski; Fiscal Year: 2013
    ..Ciliary beat frequency (CBF) will be measured by video microscopy in the trachea and nasal cavity of 3, 6, 12, and 24 month old mice...
  3. Eosinophil ORMDL3 and allergic inflammation
    P Sriramarao; Fiscal Year: 2012
    ..Using the technique of 2-color intravital video microscopy, we will examine the adhesive interaction of normal Eos versus Eos that have been rendered ORMDL3 deficient ..
  4. Anthrax Toxins Impair Phagocyte Actin-based Motility
    Frederick Southwick; Fiscal Year: 2010
    ..in infected cells, as well as cytoplasmic and reconstituted extracts, using rhodamine actin and time lapse video microscopy. D. Examine LT's effects on the in vitro function of the actin-regulatory protein Hsp27...
  5. Surveillance of Serum Contents by Mast Cells and Regulation of Allergic Immunity
    Laurence E Cheng; Fiscal Year: 2013
    ..The strategic approach will utilize advanced technologies, such as reporter antibody molecules, intravital video microscopy, and mice in which specific subsets of cells (based on lineage or function) can be deleted...
  6. L-carnitine Treatment for Vasopressor Dependent Septic Shock
    Alan E Jones; Fiscal Year: 2013
    ..If proven useful, this therapy could hasten recovery from septic shock and result in saving thousands of lives per year from this deadly disease. ..
  7. Effect of Helicobacter pylori cell shape on pathogenesis
    LAURA ELIZABETH MARTINEZ; Fiscal Year: 2013
    ..Using live video microscopy, we will record the swimming behavior and determine the directional switching frequency of the bacteria in ..
  8. Mycobacterium tuberculosis carbon metabolism in vivo
    John D McKinney; Fiscal Year: 2010
    ..Lastly, we will use microfluidics and video microscopy to analyze the real-time single-cell response of ICL-deficient bacteria after infusion of fatty acids...
  9. HS1 Function in Dendritic Cells
    Janis K Burkhardt; Fiscal Year: 2011
    ..Second, we will investigate lamellipodial and podosome dynamics in migrating BMDCs using video microscopy, ask if HS1 is required for secretion of matrix metalloproteinases, and test the ability of HS1-/- DCs to ..
  10. Temporal Requirements for Intracellular Pathogenesis
    Darren E Higgins; Fiscal Year: 2012
    ..Quantitative fluorescence and time- lapse video microscopy will be used to define the level and kinetics of expression within individual bacterial cells...
  11. HS1 Function in Dendritic Cells
    Deborah A Klos Dehring; Fiscal Year: 2010
    ..Lamellipodial and podosome dynamics will be characterized by video microscopy to determine the role of HS1 in the formation and duration of these structures...
  12. How do you build a parasite?
    John Murray; Fiscal Year: 2009
    ..the analysis of relationships between various subcellular organelles over time, using quantitative time-lapse video microscopy and image de-convolution, laser scanning confocal microscopy, fluorescence photo-bleaching and recovery, and ..
  13. Immunophilins regulate SOC entry channels in pulmonary endothelial cells
    Donna L Cioffi; Fiscal Year: 2013
    ..function;nuclear magnetic resonance spectroscopy to measure proline- containing peptide bond isomerization;video microscopy, permeability measurements and resistance measurements to measure inter-endothelial cell gap formation in ..
  14. Cell Analytics for Viability Experiments (CAVE (TM))
    Melanie M Tomczak; Fiscal Year: 2013
    ..This is also amenable to the study of cells using video microscopy and time-lapse microscopy...
  15. Control of redox regulators by the ubiquitin system
    Scott M Plafker; Fiscal Year: 2013
    ..g., siRNA, microinjection/live cell video microscopy, transcription assays), biochemical and biophysical methods (e.g...
  16. Alpha M Beta 2 integrin blockade for acute inflammatory neuropathies
    EROBOGHENE EKAMERENO UBOGU; Fiscal Year: 2013
    ..Trafficking events would be captured in real-time and quantified by video microscopy. We will also evaluate the effect of [unreadable]M integrin blockade on the behavioral, electrophysiological ..
  17. PROMISES: Progenitor Endothelial Cells as a Marker of Endothelial Injury and Repa
    Azra Bihorac; Fiscal Year: 2013
    ..As a part of this Award, she will develop an expertise in sidestream dark-field (SDF) video microscopy, a non-invasive technique that directly visualizes microcirculatory vessels and perfusion in human subjects...
  18. Hepatocellular Carcinoma in Antitrypsin Deficiency
    David H Perlmutter; Fiscal Year: 2012
    ..Globule-containing hepatocytes do not incorporate BrdU and lack signs of cell death. Second, time-lapsed video microscopy of single cells in culture show that ER accumulation of A TZ leads to profound suppression of proliferation...
  19. Preclinical evaluation of resveratrol in sepsis-induced renal injury
    JOSEPH HUNTER HOLTHOFF; Fiscal Year: 2013
    ..A very innovative aspect of this study is the use of intravital video microscopy (IWM) to study in real time the mechanisms by which capillary dysfunction develops and leads to tubular ..
  20. Determining the role of Fragile X proteins in the heart
    STEFANIE NOVAK; Fiscal Year: 2013
    ..and heartbeat parameters that include contraction strength and pacemaker activity will be measured using video microscopy and semi-automated optical heartbeat analysis software...
  21. Regulation of eNOS by Shear Stress in Intact Arteries
    Robin C Looft Wilson; Fiscal Year: 2010
    ..and concomitant diameter changes, using an isolated, cannulated, perfused mouse artery preparation and video microscopy. Arteries (mesenteric and carotid arteries) will be subjected to various magnitudes of shear stress (with or ..
  22. Entry of Oncogenic HPVs into Human Keratinocytes
    Michelle A Ozbun; Fiscal Year: 2013
    ..This will employ mixed virions comprised of HPV16 L1+HPV31 L2 (or HPV31L1+HPV16L2) and video microscopy of single particle tracking on host cells...
  23. Fibronectin peptide antagonism for chronic inflammatory neuropathies
    EROBOGHENE EKAMERENO UBOGU; Fiscal Year: 2012
    ..Trafficking events would be captured real-time and quantified by video microscopy. We will also evaluate the effect of this peptide antagonist on the behavioral, electrophysiological and ..
  24. Lateral Diffusion in an Archipelago: Monte Carlo Studies
    Michael J Saxton; Fiscal Year: 2011
    ..In single-particle tracking, computer-enhanced video microscopy is used to measure 2D motion of labeled membrane proteins or lipids on the cell surface, and 3D motion of ..
  25. Retrovirus cell-to-cell transmission
    WALTHER H MOTHES; Fiscal Year: 2013
    ..has, for the first time, documented the transmission of viruses from one cell to another using time-lapse video microscopy and shown that polarized assembly directly at the cell-cell interface can contribute to the efficient ..
  26. System for multidimensional analysis of growth and migration of neurons and neuro
    JACOB R GLASER; Fiscal Year: 2013
    ..Accordingly, the development of the Orion microscope system represents clear progress beyond the state-of-the-art, with great benefits for the neuroscience research community and society in general. ..
  27. Targeted Expression of Atoh1 in Cochlear Supporting Cells
    Mark A Parker; Fiscal Year: 2011
    ..ability for these specific supporting cell types to develop into hair cells will be observed using time lapse video microscopy, electron microscopy, and immunohistochemical expression of hair cell specific markers such as myosin 7a...
  28. hiPS Derived Cardiomyocyte Assay for Screening Toxicant Differentiation Effects
    Bradley H Garcia; Fiscal Year: 2013
    ..and yield will be determined by cytoxicity assay, and contractility measurements will be performed by video microscopy and automated image analysis...
  29. Live Imaging of Neural Crest Contribution to the Developing Cornea
    SHANNON LUCIA GRISWOLD; Fiscal Year: 2012
    ..of NCC-derived periocular cells to lens-derived signaling during cornea development by in vivo time-lapse video microscopy. 3: Determine whether lens-proximal and lens-distal NCC-derived periocular subpopulations exhibit ..
  30. NAADP-Sensitive Lysosomal Ca2+ Release Channels-TRP-ML1 in Arterial Myocytes
    Pin Lan Li; Fiscal Year: 2013
    ..NAADP-sensitive Ca2+ release channel contributes to the regulation of vascular tone and vasomotor response by video microscopy of isolated pressurized small coronary arteries...
  31. Studies of Alternations of c-MET in SCLC
    Patrick Ma; Fiscal Year: 2009
    ..The role of various inhibitors of c-Met and its mutants in SCLC would be further defined aiming to translate into novel targeted-therapeutics. ..
  32. Role of Chemorepellants during Neural Crest Migration in the Eye
    Peter Y Lwigale; Fiscal Year: 2011
    ..this hypothesis, we will combine classical embryological techniques, genetic analysis, molecular biology and video microscopy to examine the role of Npn-1 and SemaSA and visualize neural crest migration in real-time during these events...
  33. Dissecting the functions of yeast COPI - Resubmission 01
    Benjamin S Glick; Fiscal Year: 2013
    ..By combining the anchor-away method with our established video microscopy technique for visualizing yeast Golgi dynamics, we will test whether cisternal maturation requires COPI...
  34. Neuronal Spread of Herpesvirus Infection
    Lynn W Enquist; Fiscal Year: 2013
    ..Experiments are divided among three aims all featuring light and video microscopy: Imaging individual virion egress events using multi-color TIRF microscopy;assaying axon-cell egress and ..
  35. CELL AND MOLECULAR PATHOBIOLGY OF ALZHEIMER'S DISEASE
    Ralph A Nixon; Fiscal Year: 2013
    ..Innovative technologies from single-neuron gene profiling to video microscopy and high voltage immunogold EM imaging will be applied to patient cells and our novel mouse models...
  36. IN SITU POLYMERIZABLE GELS FOR DENTAL TISSUE ENGINEERING
    Antonios Mikos; Fiscal Year: 2002
    ..A three-step approach will be followed to engineer optimal in situ polymerizable hydrogels using novel video microscopy and image analysis techniques developed in our laboratory...
  37. Spore Release Mechanisms in Indoor Fungi
    NICHOLAS MONEY; Fiscal Year: 2009
    ..A combination of video microscopy and biomechanical techniques will be used to understand how the commonest indoor molds (sexual ascomycetes), ..
  38. Mechanisms of Centrosome Reproduction in Animal Cells
    EDWARD HINCHCLIFFE; Fiscal Year: 2009
    ..To continue the investigation of the cell cycle regulation of centrosome duplication we will use live-cell video microscopy and biophysical/cell biological techniques to analyze centrosome reproduction in mammalian cells and frog egg ..
  39. SELECTINS AND HEMATOPOIETIC HOMING AND HOMEOSTASIS
    Michael Long; Fiscal Year: 2000
    ..The rolling of Lin- Sca+ cells on selectins at physiological shear rates will be studied by Video microscopy. Likewise, the rolling of Lin- Sca+ on endothelial cells will be evaluated using human umbilical vein ..
  40. INTEGRIN SIGNALLING IN VASCULAR ENDOTHELIUM
    Lewis Romer; Fiscal Year: 2000
    ..Digitized video microscopy, fluorescent calcium indicators, and caged mediators of intracellular calcium flux will be used to study ..
  41. MOLECULAR MECHANISMS OF NEURONAL CELL MIGRATION
    Richard Cameron; Fiscal Year: 2000
    ..A fourth approach will be to do high resolution time-lapse video microscopy. This last assay will be performed at Yale in the laboratory of the PI's postdoctoral mentor and current ..
  42. DIFFERENTIALLY EXPRESSED GENES IN PRIMARY BREAST CANCER
    ARTHUR PARDEE; Fiscal Year: 1999
    ..By time-lapse video microscopy, we showed that motility is blocked for 12 hours when tumor cells are treated with maspin...
  43. MICROVASCULAR FLOW AND VASO-OCCLUSION OF SICKLE CELLS
    JOSEPH KURANTSINMILLS; Fiscal Year: 1991
    ..The result of these studies will provide very significant insight into the mechanisms of vaso-occlusive events in sickle cell disease...
  44. Tomographic Imaging of Cochlear Micromechanical Motions
    DENNIS FREEMAN; Fiscal Year: 2006
    ..However, current methods, such as laser Doppler vibrometry and video microscopy are limited by the low reflectivities of cochlear structures and the limited optical access provided by the ..
  45. INFLAMATION AND INFECTION ON BIOMATERIALS
    ERIC KALER; Fiscal Year: 2001
    ..These studies will be primarily carried out using an automated video microscopy system with digital image processing that allows the direct observation of individual cells interacting with ..
  46. SWELLING ACTIVATED CURRENTS AND MYOCYTE VOLUME IN CHF
    CLIVE BAUMGARTEN; Fiscal Year: 2004
    ..Perforated patch voltage clamp and digital video microscopy will be used concurrently to quantify ionic currents and their effect on cell volume...
  47. MECHANISMS OF GLUTAMATE-MEDIATED C-FOS EXPRESSION
    Steven Finkbeiner; Fiscal Year: 1999
    ..will test the role of cytoplasmic Ca2+ rises for c-fos expression using the Ca2+ -sensitive dye fura-2 and video microscopy. 2) We will test the sufficiency of NMDA receptors for c-fos induction by expressing functional NMDA ..
  48. VIDEO ENHANCED MICROSCOPY OF LIVING GROWTH CONES
    Daniel Goldberg; Fiscal Year: 1999
    ..This hypothesis will be examined by using high resolution video microscopy and immunocytochemistry to determine whether there are changes in tyrosine phosphorylation in filopodia of ..
  49. CNS DEFECT IN A MURINE ZELLWEGER SYNDROME MODEL
    Phyllis Faust; Fiscal Year: 2002
    ..The investigator will directly visualize the dynamics of migrating neurons in vitro by video microscopy using cultured brain slices...
  50. Cardiac Angiotensin: Hypertrophy and Failure
    James Morgan; Fiscal Year: 2004
    ..measurements, analysis of isolated myocyte contraction and intracellular ion regulation using fluorescence video microscopy, and confocal microscopy analysis of in situ cell morphology and apoptosis...
  51. CARDIAC METABOLISM IN HEART FAILURE
    John Williamson; Fiscal Year: 1990
    ..Further studies will utilize 31P and 1H NMR techniques to investigate post-ischemic reperfusion of rat hearts...
  52. MICROVASCULAR STUDIES IN REGENERATED SKELETAL MUSCLE
    Harold Burton; Fiscal Year: 1992
    ....
  53. AXON TARGET INTERACTIONS IN THE DEVELOPING CEREBELLUM
    Carol Mason; Fiscal Year: 2002
    ..focused arborization on individual Purkinje cells will be studied in slices of cerebellum, using time-lapse video microscopy. Third, in an in vitro model system based on purified Purkinje cells, the neural specificity of axon-target ..
  54. PiV Ultra 12 - 24 Ultra High Speed Camera for Ultrasound Microbubble Research
    John Hossack; Fiscal Year: 2009
    ..Current ultra-high-speed video microscopy systems designed for microbubble imaging include the Brandaris at Erasmus University in Rotterdam and the ..
  55. REGULATION OF MICROTUBLE BASED T CELL EFFECTOR FUNCTION
    JANIS BURKHARDT; Fiscal Year: 2003
    ..polarization, and the effects of ZAP70 and MAP4 on microtubule dynamics will be investigated in vitro using video microscopy. Aim 2 will test the role of cytoplasmic dynein in MTOC reorientation, by asking whether dynein is recruited ..
  56. CALCIUM AND CELL GROWTH
    Brian Herman; Fiscal Year: 1993
    ..Using Multiparameter Digitized Video Microscopy (MDVM) which allows the real time observation of a number of cellular functions at the level of a single ..
  57. FUSION AND NUCLEAR RESCUE DOMAINS IN DYSGENIC MICE
    JEANNE POWELL; Fiscal Year: 1991
    ..An analysis of fusion will be studied by time lapse video microscopy to describe a recently noted bubbling phenomenon characteristic of the fusion process...
  58. ROLE OF SKIN BIOMECHANICS IN MECHANORECEPTOR RESPONSE
    Mandayam Srinivasan; Fiscal Year: 2007
    ..fingerpad skin ridges and grooves using Optical Coherance Tomography, Ultrasound Backscatter Microscopy, and Video microscopy, (2) to measure the spatial distribution of pressure at the object-finger ridge contact interface using ..
  59. Age-related changes in the mechanisms of sympathetic neurotransmission in female
    HEIDI KLUESS; Fiscal Year: 2009
    ..The experimental approach to be employed is a combination of video microscopy with biosensor technology and specific adrenergic and non-adrenergic agonists and antagonists for ..
  60. RHEOLOGICAL STUDIES OF SICKLE CELL DISEASE
    LARRY MC INTIRE; Fiscal Year: 1992
    ..1) red cell- endothelial cell interactions under controlled flow conditions using epi-fluorescent video microscopy and image analysis techniques; and (2) effect of shear stress on red cell membrane permeability and the ..
  61. MURAL THROMBOSIS ON NATURAL AND ARTIFICIAL SURFACES
    Peter Dervan; Fiscal Year: 1992
    ..Epifluorescence video microscopy will be employed to visualize the thrombotic surface while in contact with blood flowing in a parallel plate ..
  62. IN VIVO IMAGING OF FORMATION OF HEPATIC METASTASES
    Jonathan Kruskal; Fiscal Year: 2002
    ..The proposed experimental approach will utilize intravital light and fluorescent video microscopy, a mouse xenograft model of human hepatic colorectal cancer metastases, fluorescent probes and two well-..