fluorescence microscopy

Summary

Summary: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.

Top Publications

  1. ncbi To see or not to see: lateral organization of biological membranes and fluorescence microscopy
    Luis A Bagatolli
    MEMPHYS Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Biochim Biophys Acta 1758:1541-56. 2006
  2. ncbi A guide to choosing fluorescent proteins
    Nathan C Shaner
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Nat Methods 2:905-9. 2005
  3. ncbi In vivo cancer targeting and imaging with semiconductor quantum dots
    Xiaohu Gao
    Department of Biomedical Engineering, and the Winship Cancer Institute, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001, Atlanta, Georgia 30322, USA
    Nat Biotechnol 22:969-76. 2004
  4. ncbi Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images
    E Meijering
    Department of Medical Informatics, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Cytometry A 58:167-76. 2004
  5. ncbi Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization
    Ahmet Yildiz
    Center for Biophysics and Computational Biology, University of Illinois, Urbana Champaign, IL 61801, USA
    Science 300:2061-5. 2003
  6. pmc Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy
    Bo Huang
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA
    Science 319:810-3. 2008
  7. ncbi Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy
    M G Gustafsson
    Department of Biochemistry and Biophysics, University of California San Francisco, California 94143 0448, USA
    J Microsc 198:82-7. 2000
  8. ncbi Feature point tracking and trajectory analysis for video imaging in cell biology
    I F Sbalzarini
    Institute of Computational Science, ETH Zurich, 8092 Zurich, Switzerland
    J Struct Biol 151:182-95. 2005
  9. pmc A base-excision DNA-repair protein finds intrahelical lesion bases by fast sliding in contact with DNA
    Paul C Blainey
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 103:5752-7. 2006
  10. ncbi In vivo imaging of quantum dots encapsulated in phospholipid micelles
    Benoit Dubertret
    Center for Studies in Physics and Biology, Laboratory of Molecular Embryology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 298:1759-62. 2002

Research Grants

  1. Genetic variation and regulatory networks: Mechanisms and complexity
    DANA PE ER; Fiscal Year: 2009
  2. Structural and molecular basis of drug-induced IKACh reduction
    SAMI FOUAD NOUJAIM; Fiscal Year: 2012
  3. SAMI FOUAD NOUJAIM; Fiscal Year: 2014
  4. JULIAN G CAMBRONERO; Fiscal Year: 2016
  5. Regulation of the Endothelial Citrulline-Nitric Oxide Cycle
    Duane C Eichler; Fiscal Year: 2010
  6. Site-directed Chemotherapy for Breast Cancer using Novel Angiogenesis Inhibitor
    Shaker A Mousa; Fiscal Year: 2010
  7. Biophysics of the morphology and motility of Borrelia burgdorferi in diverse envi
    Charles W Wolgemuth; Fiscal Year: 2013
  8. Visualization of ototoxic mitochondrial dysfunction in mammalian hair cells
    Shelley A Batts; Fiscal Year: 2012
  9. ANP Receptor: Molecular approach of signaling mechanisms
    Kailash N Pandey; Fiscal Year: 2013
  10. COREY B SMITH; Fiscal Year: 2015

Detail Information

Publications340 found, 100 shown here

  1. ncbi To see or not to see: lateral organization of biological membranes and fluorescence microscopy
    Luis A Bagatolli
    MEMPHYS Center for Biomembrane Physics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense M, Denmark
    Biochim Biophys Acta 1758:1541-56. 2006
    In the last few years several experimental strategies based on epi-, confocal and two photon excitation fluorescence microscopy techniques have been employed to study the lateral structure of membranes using giant vesicles as model ..
  2. ncbi A guide to choosing fluorescent proteins
    Nathan C Shaner
    Department of Pharmacology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Nat Methods 2:905-9. 2005
    ....
  3. ncbi In vivo cancer targeting and imaging with semiconductor quantum dots
    Xiaohu Gao
    Department of Biomedical Engineering, and the Winship Cancer Institute, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001, Atlanta, Georgia 30322, USA
    Nat Biotechnol 22:969-76. 2004
    ..These results raise new possibilities for ultrasensitive and multiplexed imaging of molecular targets in vivo...
  4. ncbi Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images
    E Meijering
    Department of Medical Informatics, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Cytometry A 58:167-76. 2004
    ..To facilitate this task, we developed a semiautomatic neurite tracing technique. This article describes the design and validation of the technique...
  5. ncbi Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization
    Ahmet Yildiz
    Center for Biophysics and Computational Biology, University of Illinois, Urbana Champaign, IL 61801, USA
    Science 300:2061-5. 2003
    ..These results strongly support a hand-over-hand model of motility, not an inchworm model...
  6. pmc Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy
    Bo Huang
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA
    Science 319:810-3. 2008
    Recent advances in far-field fluorescence microscopy have led to substantial improvements in image resolution, achieving a near-molecular resolution of 20 to 30 nanometers in the two lateral dimensions...
  7. ncbi Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy
    M G Gustafsson
    Department of Biochemistry and Biophysics, University of California San Francisco, California 94143 0448, USA
    J Microsc 198:82-7. 2000
    ..Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes...
  8. ncbi Feature point tracking and trajectory analysis for video imaging in cell biology
    I F Sbalzarini
    Institute of Computational Science, ETH Zurich, 8092 Zurich, Switzerland
    J Struct Biol 151:182-95. 2005
    ..The algorithm is well suited for video imaging in cell biology relying on low-intensity fluorescence microscopy. Its applicability is demonstrated in three case studies involving transport of low-density lipoproteins ..
  9. pmc A base-excision DNA-repair protein finds intrahelical lesion bases by fast sliding in contact with DNA
    Paul C Blainey
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 103:5752-7. 2006
    ..5 kcal/mol (1 kcal = 4.2 kJ). This nearly barrierless Brownian sliding indicates that DNA glycosylases locate lesion bases by a massively redundant search in which the enzyme selectively binds 8-oxoguanine under kinetic control...
  10. ncbi In vivo imaging of quantum dots encapsulated in phospholipid micelles
    Benoit Dubertret
    Center for Studies in Physics and Biology, Laboratory of Molecular Embryology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 298:1759-62. 2002
    ..Nanocrystal fluorescence could be followed to the tadpole stage, allowing lineage-tracing experiments in embryogenesis...
  11. ncbi Reconstitution of a microtubule plus-end tracking system in vitro
    Peter Bieling
    European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nature 450:1100-5. 2007
    ..Using time-lapse total internal reflection fluorescence microscopy, we show that the EB1 homologue Mal3 has an enhanced affinity for growing microtubule end structures as ..
  12. ncbi Development and use of fluorescent protein markers in living cells
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 300:87-91. 2003
    ....
  13. ncbi Control of the assembly of ATP- and ADP-actin by formins and profilin
    David R Kovar
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Cell 124:423-35. 2006
    ..We used total internal reflection fluorescence microscopy to observe directly individual actin filament polymerization in the presence of two mammalian formins (..
  14. ncbi Autophagy controls Salmonella infection in response to damage to the Salmonella-containing vacuole
    Cheryl L Birmingham
    Infection, Immunity, Injury and Repair Program, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 281:11374-83. 2006
    ..Our findings support a role for autophagy in innate immunity and demonstrate that Salmonella infection is a powerful model to study the autophagy process...
  15. pmc Automated three-dimensional detection and shape classification of dendritic spines from fluorescence microscopy images
    Alfredo Rodriguez
    Department of Neuroscience, Mount Sinai School of Medicine, New York, New York, United States of America
    PLoS ONE 3:e1997. 2008
    ..The technique opens new horizons on the objective evaluation of spine changes with synaptic plasticity, normal development and aging, and with neurodegenerative disorders that impair cognitive function...
  16. pmc Visualizing infection of individual influenza viruses
    Melike Lakadamyali
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 100:9280-5. 2003
    ..the transport, acidification, and fusion of single influenza viruses in living cells by using real-time fluorescence microscopy and have dissected individual stages of the viral entry pathway...
  17. ncbi Looking and listening to light: the evolution of whole-body photonic imaging
    Vasilis Ntziachristos
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School CNY149, 13th Street 5406, Charlestown, Massachusetts 02129, USA
    Nat Biotechnol 23:313-20. 2005
    ..For photonic imaging to fully realize its potential, however, further progress will be needed in refining optical inversion methods and data acquisition techniques...
  18. ncbi Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review
    Karen R Steingart
    Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital, University of California, San Francisco, CA, USA
    Lancet Infect Dis 6:570-81. 2006
    ..In high-income countries, fluorescence microscopy rather than conventional microscopy is the standard diagnostic method...
  19. ncbi Quantum dot bioconjugates for imaging, labelling and sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Nat Mater 4:435-46. 2005
    ..The potential of QDs in biology has just begun to be realized and new avenues will arise as our ability to manipulate these materials improves...
  20. pmc Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes
    Andreas Vonderheit
    Institute of Biochemistry, Swiss Federal Institute of Technology, Zurich, Switzerland
    PLoS Biol 3:e233. 2005
    ..was followed using immunofluorescence in untransfected cells, and by video-enhanced, triple-color fluorescence microscopy in live cells transfected with GFP- and RFP-tagged Rab5, Rab7, Rab4, and Arf1...
  21. pmc Imaging the biogenesis of individual HIV-1 virions in live cells
    Nolwenn Jouvenet
    Aaron Diamond AIDS Research Center, The Rockefeller University, New York, New York 10065, USA
    Nature 454:236-40. 2008
    ..These approaches allow a previously unobserved view of the genesis of individual virions and the determination of parameters of viral assembly that are inaccessible with conventional techniques...
  22. pmc Super-resolution fluorescence microscopy
    Bo Huang
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA
    Annu Rev Biochem 78:993-1016. 2009
    ..resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional ..
  23. ncbi Dynamic binding of histone H1 to chromatin in living cells
    T Misteli
    National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Nature 408:877-81. 2000
    ..These results support a model in which linker histones bind dynamically to chromatin in a stop-and-go mode...
  24. pmc Single-molecule imaging of RNA polymerase-DNA interactions in real time
    Y Harada
    Biomotron Project, ERATO, JST, Senba Higashi 2 4 14, Mino, Osaka 562 0035, Japan
    Biophys J 76:709-15. 1999
    Using total internal reflection fluorescence microscopy, we have directly observed individual interactions of single RNA polymerase molecules with a single molecule of lambda-phage DNA suspended in solution by optical traps...
  25. ncbi Directed proteomic analysis of the human nucleolus
    Jens S Andersen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230, Odense M, Denmark
    Curr Biol 12:1-11. 2002
    ..Recent studies suggest it may also have roles in RNA transport, RNA modification, and cell cycle regulation. Despite over 150 years of research into nucleoli, many aspects of their structure and function remain uncharacterized...
  26. ncbi Creating new fluorescent probes for cell biology
    Jin Zhang
    Department of Pharmacology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 18 496, Cambridge, Massachusetts 02139, USA
    Nat Rev Mol Cell Biol 3:906-18. 2002
    ....
  27. ncbi Real-time kinetics of gene activity in individual bacteria
    Ido Golding
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 123:1025-36. 2005
    ..This greatly facilitates kinetic interpretations in terms of the integer-valued random processes that produce the fluctuations...
  28. ncbi Cortical actin organization: lessons from ERM (ezrin/radixin/moesin) proteins
    S Tsukita
    College of Medical Technology, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Biol Chem 274:34507-10. 1999
  29. ncbi A neural network classifier capable of recognizing the patterns of all major subcellular structures in fluorescence microscope images of HeLa cells
    M V Boland
    Center for Light Microscope Imaging and Biotechnology, Biomedical and Health Engineering Program, Carnegie Mellon University, 4400 Fifth Ave, Pittsburgh, PA 15213, USA
    Bioinformatics 17:1213-23. 2001
    ..Images for ten different subcellular patterns (including all major organelles) were collected using fluorescence microscopy. The patterns were described using a variety of numeric features, including Zernike moments, Haralick ..
  30. ncbi Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation
    Chang Deng Hu
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Cell 9:789-98. 2002
    ..These results attest to the general applicability of the BiFC assay for studies of protein interactions...
  31. pmc Multicolor super-resolution imaging with photo-switchable fluorescent probes
    Mark Bates
    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
    Science 317:1749-53. 2007
    ..Using this approach, we demonstrate multicolor imaging of DNA model samples and mammalian cells with 20- to 30-nanometer resolution. This technique will facilitate direct visualization of molecular interactions at the nanometer scale...
  32. pmc Lifeact: a versatile marker to visualize F-actin
    Julia Riedl
    Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Methods 5:605-7. 2008
    ..Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells...
  33. ncbi Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor
    E C Dell'Angelica
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 3:11-21. 1999
    ..Our results suggest that AP-3 functions in protein sorting to lysosomes and provide an example of a human disease in which altered trafficking of integral membrane proteins is due to mutations in a component of the sorting machinery...
  34. pmc Cortical recruitment of nonmuscle myosin II in early syncytial Drosophila embryos: its role in nuclear axial expansion and its regulation by Cdc2 activity
    Anne Royou
    Centre National de la Recherche Scientifique, Centre de Genetique Moleculaire, 91198 Gif sur Yvette, France
    J Cell Biol 158:127-37. 2002
    ..These studies suggest a model in which cycles of myosin-mediated contraction and relaxation, tightly linked to Cdc2 and Rho kinase activity, are directly responsible for the axial expansion of the syncytial nuclei...
  35. pmc Filopodia and actin arcs guide the assembly and transport of two populations of microtubules with unique dynamic parameters in neuronal growth cones
    Andrew W Schaefer
    Yale University, New Haven, CT 06520, USA
    J Cell Biol 158:139-52. 2002
    ..Actin arcs interact with MTs and transport them into the C-domain. Interestingly, although the MTs associated with arcs are less dynamic than P-domain MTs, they elongate efficiently as a result of markedly lower catastrophe frequencies...
  36. pmc Analysis of the dynein-dynactin interaction in vitro and in vivo
    Stephen J King
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Mol Biol Cell 14:5089-97. 2003
    ..The dynein IC fragments have different effects on endomembrane localization, suggesting that different endomembranes may bind dynein via distinct mechanisms...
  37. pmc Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping
    Sungjee Kim
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Biotechnol 22:93-7. 2004
    ..Taken together, the chemical, optical and in vivo data presented in this study demonstrate the potential of near-infrared quantum dots for biomedical imaging...
  38. ncbi Fluorescence microscopy
    Jeff W Lichtman
    Department of Molecular and Cell Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA
    Nat Methods 2:910-9. 2005
    Although fluorescence microscopy permeates all of cell and molecular biology, most biologists have little experience with the underlying photophysical phenomena...
  39. ncbi Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions
    Y John Shyu
    Purdue University, West Lafayette IN 47907, USA
    Biotechniques 40:61-6. 2006
    ..These newly identified fluorescent protein fragments will facilitate the study of protein-protein interactions in living cells and whole animals under physiological conditions...
  40. ncbi Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype
    A G Uren
    The Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, 3050, Victoria, Australia
    Curr Biol 10:1319-28. 2000
    ..To determine the function of Survivin in mammals, we analyzed the pattern of localization of Survivin protein during the cell cycle, and deleted its gene by homologous recombination in mice...
  41. ncbi Real-time single-molecule imaging of the infection pathway of an adeno-associated virus
    G Seisenberger
    Department Chemie, , Butenandtstrasse 11, , Germany
    Science 294:1929-32. 2001
    ..The real-time visualization of the infection pathway of single AAVs shows a much faster infection than was generally observed so far...
  42. ncbi The complement regulator factor H binds to the surface protein OspE of Borrelia burgdorferi
    J Hellwage
    Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014 Helsinki, Finland
    J Biol Chem 276:8427-35. 2001
    ..Specific binding of factor H to B. burgdorferi sensu stricto OspE may help the pathogen to evade complement attack and phagocytosis...
  43. pmc Real-time measurements of actin filament polymerization by total internal reflection fluorescence microscopy
    Jeffrey R Kuhn
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Biophys J 88:1387-402. 2005
    ..The only assay meeting these criteria is total internal reflection fluorescence microscopy (Amann and Pollard, 2001; Fujiwara et al., 2002)...
  44. ncbi Single-cell quantification of molecules and rates using open-source microscope-based cytometry
    Andrew Gordon
    The Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, California 94704, USA
    Nat Methods 4:175-81. 2007
    ..Owing to their sensitivity, accuracy and ability to track changes in individual cells over time, these microscope methods may complement flow-cytometric measurements for studies of the quantitative physiology of cellular systems...
  45. doi Chapter 9: Counting proteins in living cells by quantitative fluorescence microscopy with internal standards
    Jian Qiu Wu
    Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA
    Methods Cell Biol 89:253-73. 2008
    ..This chapter is meant as a resource for cell biologists, biochemists, and biophysicists interested in quantifying macromolecules involved in their favorite molecular pathways in live cells...
  46. doi Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy
    Kirill Kolmakov
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Chemistry 16:158-66. 2010
    ..g., amines and thiols) in complex mixtures. High-resolution GSDIM images and live-cell STED-FCS experiments on labeled microtubules and lipids prove the versatility of the novel probes for modern fluorescence microscopy and nanoscopy.
  47. ncbi In vivo molecular and cellular imaging with quantum dots
    Xiaohu Gao
    Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001, Atlanta, GA 30322, USA
    Curr Opin Biotechnol 16:63-72. 2005
    ..Bioconjugated QDs have raised new possibilities for ultrasensitive and multiplexed imaging of molecular targets in living cells, animal models and possibly in humans...
  48. ncbi Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail
    Junichi Ikenouchi
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Sci 116:1959-67. 2003
    ..e. the expression of adherens and tight junction adhesion molecules, respectively)...
  49. ncbi The glucocorticoid receptor: rapid exchange with regulatory sites in living cells
    J G McNally
    Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, National Cancer Institute, Bethesda, MD 20892 5055, USA
    Science 287:1262-5. 2000
    ..Thus, the interaction of regulatory proteins with target sites in chromatin is a more dynamic process than previously believed...
  50. pmc Rapid cycling of lipid raft markers between the cell surface and Golgi complex
    B J Nichols
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20895, USA
    J Cell Biol 153:529-41. 2001
    ..The PM-Golgi cycling pathway followed by GPI-GFP could serve to regulate lipid raft distribution and function within cells...
  51. pmc RhoA is required for monocyte tail retraction during transendothelial migration
    R A Worthylake
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 154:147-60. 2001
    ..Finally, we find that p160ROCK signaling negatively regulates integrin adhesions and that inhibition of RhoA results in an accumulation of beta2 integrin in the unretracted tails...
  52. ncbi Intravital microscopy demonstrating antibody-mediated immobilisation of Plasmodium berghei sporozoites injected into skin by mosquitoes
    Jerome P Vanderberg
    Department of Medical and Molecular Parasitology, New York University School of Medicine, 341 East 25th Street, New York, NY 10010, USA
    Int J Parasitol 34:991-6. 2004
    ....
  53. pmc Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins
    Michael Hofmann
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Proc Natl Acad Sci U S A 102:17565-9. 2005
    b>Fluorescence microscopy is indispensable in many areas of science, but until recently, diffraction has limited the resolution of its lens-based variant...
  54. pmc Accuracy and precision in quantitative fluorescence microscopy
    Jennifer C Waters
    Harvard Medical School, Department of Cell Biology, Boston, MA 02115, USA
    J Cell Biol 185:1135-48. 2009
    ..and development of genetically encoded fluorophores, there has been a huge increase in the use of fluorescence microscopy to quantify spatial and temporal measurements of fluorescent molecules in biological specimens...
  55. ncbi Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility
    H L Zhang
    Department of Neuroscience, Rose F. Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Neuron 31:261-75. 2001
    ..Growth cones exhibited impaired motility in the presense of antisense. These results suggest a novel mechanism to influence growth cone dynamics involving the regulated transport of mRNA...
  56. ncbi Insulin-stimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation
    Hiroyuki Sano
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 278:14599-602. 2003
    ..These findings strongly indicate that insulin-stimulated phosphorylation of AS160 is required for GLUT4 translocation and that this phosphorylation signals translocation through inactivation of the Rab GAP function...
  57. pmc Stress fibers are generated by two distinct actin assembly mechanisms in motile cells
    Pirta Hotulainen
    Institute of Biotechnology, University of Helsinki, Helsinki FI 00014, Finland
    J Cell Biol 173:383-94. 2006
    ..Based on these data, we propose a general model for assembly and maintenance of contractile actin structures in cells...
  58. ncbi The multifunctional nucleolus
    Francois Michel Boisvert
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    Nat Rev Mol Cell Biol 8:574-85. 2007
    ....
  59. ncbi Compartmentalization of beta-secretase (Asp2) into low-buoyant density, noncaveolar lipid rafts
    D R Riddell
    Neurology Centre of Excellence for Drug Discovery, GlaxoSmithKline, New Frontiers Science Park, Third Avenue, Harlow CM19 5AW, United Kingdom
    Curr Biol 11:1288-93. 2001
    ..These observations are consistent with the raft localization of APP processing and suggest that the partitioning of Asp2 into lipid rafts may underlie the cholesterol sensitivity of beta-amyloid production...
  60. ncbi FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells
    S Bolte
    Dynamique de la Compartimentation Cellulaire, Institut des Sciences du Vegetal, CNRS UPR 2355, Gif sur Yvette, 91198 Cedex, France
    J Microsc 214:159-73. 2004
    ..Particular emphasis is placed on using the FM-dyes in double labelling experiments to identity specific organelles. In this way, staining of the Golgi with FM4-64 has been demonstrated for the first time...
  61. ncbi Analysis of cycloheximide-induced apoptosis in human leukocytes: fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
    Dejan Baskic
    Institute of Microbiology and Immunology, School of Medicine, University of Kragujevac, Serbia and Montenegro
    Cell Biol Int 30:924-32. 2006
    ..used for detecting apoptosis were light microscopy (May Grunwald-Giemsa and trypan blue staining), fluorescence microscopy (acridin orange/ethidium bromide and annexin V/propidium iodide staining) and agarose gel electrophoresis ..
  62. doi Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy
    Christopher Gell
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Methods Cell Biol 95:221-45. 2010
    ..The use of total internal reflection fluorescence microscopy with fluorescently labeled tubulin and MAPs has allowed us to study microtubule dynamics at the ..
  63. ncbi Cohesin cleavage by separase required for anaphase and cytokinesis in human cells
    S Hauf
    Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Science 293:1320-3. 2001
    ..Our results indicate that cohesin cleavage by separase is essential for sister chromatid separation and for the completion of cytokinesis...
  64. pmc A comparative study of gallstones from children and adults using FTIR spectroscopy and fluorescence microscopy
    Oleg Kleiner
    Department of Physics, Ben Gurion University, Beer Sheva, 84105, Israel
    BMC Gastroenterol 2:3. 2002
    ..Recent years has seen an increasing trend in the number of cases involving children. The focus of this study is on the analysis of the chemical composition of gallstones from child and adult patients using spectroscopic methods...
  65. ncbi A CBP binding transcriptional repressor produced by the PS1/epsilon-cleavage of N-cadherin is inhibited by PS1 FAD mutations
    Philippe Marambaud
    Department of Psychiatry and Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Cell 114:635-45. 2003
    ..These data raise the possibility that FAD mutation-induced transcriptional abnormalities maybe causally related to the dementia associated with FAD...
  66. ncbi A molecular throttle: the recombination hotspot chi controls DNA translocation by the RecBCD helicase
    Maria Spies
    Section of Microbiology, Center for Genetics and Development, University of California, Davis 9561, USA
    Cell 114:647-54. 2003
    ..We propose that interaction with chi results in an enzyme in which one of the two motor subunits, likely the RecD motor, is uncoupled from the holoenzyme to produce the slower translocase...
  67. pmc Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression
    Yoshihiro H Inoue
    Drosophila Genetic Resource Center, Kyoto Institute of Technology, Sagaippongi cho, Ukyou ku, 616 8354, Japan
    J Cell Biol 166:49-60. 2004
    ..Our work identifies a new function for Orbit/Mast/CLASP and identifies a novel MT population involved in cleavage furrow initiation...
  68. ncbi Measurement of SOS expression in individual Escherichia coli K-12 cells using fluorescence microscopy
    Jesse D McCool
    Department of Microbiology, Morrill Science Center IV N203, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    Mol Microbiol 53:1343-57. 2004
    ..These results are discussed in a context of how the processes of DNA replication and recombination may affect cells in a population differentially...
  69. ncbi High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy
    Peter J Verveer
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Methods 4:311-3. 2007
    ..a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples...
  70. doi High-speed imaging of developing heart valves reveals interplay of morphogenesis and function
    Paul J Scherz
    Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA
    Development 135:1179-87. 2008
    ..Here, we use high-speed fluorescence microscopy to investigate AVV morphogenesis in zebrafish at cellular resolution...
  71. pmc High-speed, miniaturized fluorescence microscopy in freely moving mice
    Benjamin A Flusberg
    James H Clark Center for Biomedical Engineering and Sciences, Stanford University, 318 Campus Drive, Stanford, California 94305, USA
    Nat Methods 5:935-8. 2008
    ..We describe a miniaturized (1.1 g mass) epifluorescence microscope for cellular-level brain imaging in freely moving mice, and its application to imaging microcirculation and neuronal Ca(2+) dynamics...
  72. pmc Identification of novel graded polarity actin filament bundles in locomoting heart fibroblasts: implications for the generation of motile force
    L P Cramer
    The Randall Institute, Kings College London, University of London, United Kingdom
    J Cell Biol 136:1287-305. 1997
    ..The organization, dynamics, and relative abundance of graded polarity actin filament bundles have important implications for the generation of motile force during primary heart fibroblast locomotion...
  73. ncbi Probing gene expression in live cells, one protein molecule at a time
    Ji Yu
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Science 311:1600-3. 2006
    ..The quantitative study of low-level gene expression demonstrates the potential of single-molecule experiments in elucidating the workings of fundamental biological processes in living cells...
  74. ncbi Biological applications of X-ray fluorescence microscopy: exploring the subcellular topography and speciation of transition metals
    Christoph J Fahrni
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332 0400, USA
    Curr Opin Chem Biol 11:121-7. 2007
    Synchrotron X-ray fluorescence microscopy (SXRF) is a microanalytical technique for the quantitative mapping of elemental distributions...
  75. ncbi The polyphosphate bodies of Chlamydomonas reinhardtii possess a proton-pumping pyrophosphatase and are similar to acidocalcisomes
    F A Ruiz
    Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 276:46196-203. 2001
    ..Polyphosphate was also localized to the contractile vacuole by 4',6-diamidino-2-phenylindole staining, suggesting, with the immunochemical data, a link between these organelles and the acidocalcisomes...
  76. ncbi Single-molecule visualization in cell biology
    Yasushi Sako
    Nanobiology Laboratories, Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
    Nat Rev Mol Cell Biol . 2003
    ..In this article, we discuss how single-molecule visualization can be used in cell biology...
  77. ncbi UV-induced ataxia-telangiectasia-mutated and Rad3-related (ATR) activation requires replication stress
    Irene M Ward
    Guggenheim 1306, Division of Oncology Research, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 279:9677-80. 2004
    ..Phosphorylated H2AX might further facilitate ATR activation by stabilizing ATR at the sites of arrested replication forks...
  78. pmc Using single-particle tracking to study nuclear trafficking of viral genes
    Hazen P Babcock
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Biophys J 87:2749-58. 2004
    ..technique that allows gene trafficking to be studied at the single-gene level by combining sensitive fluorescence microscopy with microinjection...
  79. ncbi Nuclear import of the BTB/POZ transcriptional regulator Kaiso
    Kevin F Kelly
    Department of Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada
    J Cell Sci 117:6143-52. 2004
    ..The identification of a Kaiso NLS thus clarifies the mechanism by which Kaiso translocates to the nucleus to regulate transcription of genes with diverse roles in cell growth and development...
  80. pmc The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility
    Mijung Kwon
    University of California Davis, Davis, California 95616, USA
    Mol Biol Cell 15:219-33. 2004
    ..We propose that KLP3A acts on MTs associated with chromosome arms and the central spindle to organize ipMT bundles, to drive spindle pole separation and to facilitate chromatid motility...
  81. pmc Imaging inflammation of the pancreatic islets in type 1 diabetes
    Maria C Denis
    Section on Immunology and Immunogenetics, Joslin Diabetes Center, 1 Joslin Place, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 101:12634-9. 2004
    ..We could detect the onset and evolution of insulitis in vivo and in real time, permitting us to study the natural history of diabetes in individual animals...
  82. ncbi Two distinct actin networks drive the protrusion of migrating cells
    A Ponti
    Department of Cell Biology, Scripps Research Institute TSRI, La Jolla, CA 92037, USA
    Science 305:1782-6. 2004
    ..Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion...
  83. ncbi Visualizing dendritic cell networks in vivo
    Randall L Lindquist
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Nat Immunol 5:1243-50. 2004
    ..Mature DCs were more motile than steady-state DCs and were rapidly dispersed and integrated into the sessile network, facilitating their interaction with migrating T cells...
  84. pmc Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis
    M Mancini
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 149:603-12. 2000
    ..We propose that the Golgi complex, like mitochondria, senses and integrates unique local conditions, and transduces pro-apoptotic signals through local caspases, which regulate local effectors...
  85. ncbi Cell proliferation without neurogenesis in adult primate neocortex
    D R Kornack
    Department of Neurobiology, Yale University School of Medicine, New Haven, CT 06510, USA
    Science 294:2127-30. 2001
    ..Thus, our results do not substantiate the claim of neurogenesis in normal adult primate neocortex...
  86. ncbi Cell-surface attachment of pedestal-forming enteropathogenic E. coli induces a clustering of raft components and a recruitment of annexin 2
    Nicole Zobiack
    Institute for Medical Biochemistry, Center for Molecular Biology of Inflammation, Von Esmarch Str 56, D 48149 Munster, Germany
    J Cell Sci 115:91-8. 2002
    ....
  87. ncbi Zic2 patterns binocular vision by specifying the uncrossed retinal projection
    Eloisa Herrera
    Department of Pathology and Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA
    Cell 114:545-57. 2003
    ..These data provide evidence for transcriptional coding of axon pathfinding at the midline...
  88. pmc Macromolecular-scale resolution in biological fluorescence microscopy
    Gerald Donnert
    Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37070 Gottingen, Germany
    Proc Natl Acad Sci U S A 103:11440-5. 2006
    We demonstrate far-field fluorescence microscopy with a focal-plane resolution of 15-20 nm in biological samples...
  89. pmc Circulating blood cells function as a surveillance system for damaged tissue in Drosophila larvae
    Daniel T Babcock
    Department of Biochemistry and Molecular Biology, University of Texas Graduate School of Biomedical Sciences, University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:10017-22. 2008
    ....
  90. pmc Visualization of molecular interactions using bimolecular fluorescence complementation analysis: characteristics of protein fragment complementation
    Tom K Kerppola
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109 0650, USA
    Chem Soc Rev 38:2876-86. 2009
    ..The review will be of interest to scientists interested in the investigation of macromolecular interactions or modifications who need exquisite sensitivity for the detection of their complexes or conjugates of interest...
  91. ncbi The role of apoptosis in creating and maintaining luminal space within normal and oncogene-expressing mammary acini
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 111:29-40. 2002
    ..Hence, apoptosis can counteract increased proliferation to maintain luminal space, suggesting that tumor cells must restrain apoptosis to populate the lumen...
  92. ncbi Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation
    H Zhou
    Division of Pathology and Laboratory Medicine, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Nat Genet 20:189-93. 1998
    ..These findings suggest that STK15 is a critical kinase-encoding gene, whose overexpression leads to centrosome amplification, chromosomal instability and transformation in mammalian cells...
  93. ncbi Asymmetric loading of Kar9 onto spindle poles and microtubules ensures proper spindle alignment
    Dimitris Liakopoulos
    Institute of Biochemistry, Swiss Federal Institute of Technology, ETH Hoenggerberg, HPM, CH 8093 Zurich, Switzerland
    Cell 112:561-74. 2003
    ..In turn, asymmetric distribution of Kar9 ensures that only one pole orients toward the bud. Our results indicate that Cdk1-dependent spindle asymmetry ensures proper alignment of the mitotic spindle with the cell division axis...
  94. ncbi Bst-2/HM1.24 is a raft-associated apical membrane protein with an unusual topology
    Sabine Kupzig
    Department of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    Traffic 4:694-709. 2003
    ..Data from several experiments are consistent with this localization. We present a model in which BST-2/HM1.24 serves to link adjacent lipid rafts within the plasma membrane...
  95. ncbi MR molecular imaging and fluorescence microscopy for identification of activated tumor endothelium using a bimodal lipidic nanoparticle
    Willem J M Mulder
    Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, MB Eindhoven, The Netherlands
    FASEB J 19:2008-10. 2005
    ..of alphavbeta3 in tumor bearing mice with a combination of magnetic resonance imaging (MRI) and fluorescence microscopy. To that end, we prepared MR-detectable and fluorescent liposomes, which carry approximately 700 ..
  96. pmc Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes
    Hari Shroff
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Proc Natl Acad Sci U S A 104:20308-13. 2007
    ..The simplicity, minimal invasiveness, resolution, and speed of the technique all suggest its potential to directly visualize molecular interactions within cellular structures at the nanometer scale...
  97. pmc Microtubule plus-end tracking by CLIP-170 requires EB1
    Ram Dixit
    Department of Physiology and Pennsylvania Muscle Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 106:492-7. 2009
    ..EB1 and CLIP-170 in vitro at single-molecule resolution using time-lapse total internal reflection fluorescence microscopy. We found that EB1 is capable of dynamically tracking growing microtubule plus-ends...
  98. pmc Localization accuracy in single-molecule microscopy
    Raimund J Ober
    Department of Electrical Engineering, University of Texas at Dallas, Richardson, Texas, USA
    Biophys J 86:1185-200. 2004
    ..The results demonstrate what is achievable by single-molecule microscopy and provide guidelines for experimental design...
  99. ncbi Cell damage and reactive oxygen species production induced by fluorescence microscopy: effect on mitosis and guidelines for non-invasive fluorescence microscopy
    Ram Dixit
    The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA
    Plant J 36:280-90. 2003
    ..technology makes it possible to view dynamic processes in living cells, but extended observation, using fluorescence microscopy (both wide-field and confocal), can result in significant light energy exposure...
  100. pmc Echovirus 1 endocytosis into caveosomes requires lipid rafts, dynamin II, and signaling events
    Vilja Pietiäinen
    Department of Virology, Haartman Institute, University of Helsinki, FIN 00014 Helsinki, Finland
    Mol Biol Cell 15:4911-25. 2004
    ..These results shed further light on the cell entry of nonenveloped viral pathogens and illustrate the use of viruses as probes to dissect caveolin-associated endocytic pathways...
  101. pmc Tocopherols play a crucial role in low-temperature adaptation and Phloem loading in Arabidopsis
    Hiroshi Maeda
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
    Plant Cell 18:2710-32. 2006
    ..Together, these results indicate that tocopherols have a more limited role in photoprotection than previously assumed but play crucial roles in low-temperature adaptation and phloem loading...

Research Grants72

  1. Genetic variation and regulatory networks: Mechanisms and complexity
    DANA PE ER; Fiscal Year: 2009
    ..These tools will be made publicly available, including a friendly graphical user interface and visualization. ..
  2. Structural and molecular basis of drug-induced IKACh reduction
    SAMI FOUAD NOUJAIM; Fiscal Year: 2012
    ..To achieve my aims, I will use a multidisciplinary approach, involving fluorescence microscopy, chemiluminescence, NMR spectroscopy, X-ray crystallography and electrophysiology...
  3. SAMI FOUAD NOUJAIM; Fiscal Year: 2014
    ..To achieve my aims, I will use a multidisciplinary approach, involving fluorescence microscopy, chemiluminescence, NMR spectroscopy, X-ray crystallography and electrophysiology...
  4. JULIAN G CAMBRONERO; Fiscal Year: 2016
    ..and the adaptors Grb2 and 14-3-3 through biochemical, genetic (Rac2-/-, WASp-/- and PLD2-/- KO mice) and fluorescence microscopy experiments...
  5. Regulation of the Endothelial Citrulline-Nitric Oxide Cycle
    Duane C Eichler; Fiscal Year: 2010
    ..inhibitors in conjunction with siRNA;3) functional studies via enzyme activity and NO assays;4) fluorescence microscopy;5) real time PCR and western blotting;and 6) promoter analysis using mutagenesis, luciferase assays and ..
  6. Site-directed Chemotherapy for Breast Cancer using Novel Angiogenesis Inhibitor
    Shaker A Mousa; Fiscal Year: 2010
    ..Preliminary data using fluorescence microscopy showed that fluorescein-loaded TclNPs localize preferentially at the plasma membrane of the breast cancer ..
  7. Biophysics of the morphology and motility of Borrelia burgdorferi in diverse envi
    Charles W Wolgemuth; Fiscal Year: 2013
    ..Finally, modeling and time-lapse fluorescence microscopy will be used to determine the mechanisms of motility in epithelial cell layers, and in the tick and mouse...
  8. Visualization of ototoxic mitochondrial dysfunction in mammalian hair cells
    Shelley A Batts; Fiscal Year: 2012
    ..To define the role of ototoxic mitochondrial dysfunction in hair cell apoptosis, I will use fluorescence microscopy techniques in adult guinea pigs in vivo and in postnatal rat cochlear cultures in vitro...
  9. ANP Receptor: Molecular approach of signaling mechanisms
    Kailash N Pandey; Fiscal Year: 2013
    ..aspects of NPRA including the dynamics of internalization, trafficking, sequestration, and recycling by fluorescence microscopy in living cells in real time, and 4) delineate the molecular determinants mediating post-binding events ..
  10. COREY B SMITH; Fiscal Year: 2015
    ..of these aims, we employ state of the art electrophysiological, electrochemical and quantitative fluorescence microscopy as well as a newly-developed silicon nanowire-based field effect transistor (SiNW-FET) biosensor to ..
  11. Regulation of the VDJ recombinase during genotoxic stress
    Karla K Rodgers; Fiscal Year: 2013
    ..In this project, we will use fluorescence microscopy combined with assays to monitor cellular protein-protein and protein-DNA interactions...
  12. Mechanism and therapy for arteriovenous malformation
    Suk Paul Oh; Fiscal Year: 2013
    ..Deep-penetrating and noninvasive multiphoton fluorescence microscopy will be employed to capture 3-dimensional time lapse images blood vessels at subcellular level during AVM ..
  13. Characterizing gene regulation with single molecule sensitive probes
    Philip J Santangelo; Fiscal Year: 2013
    ..is our contention that when combined with antibody-based proximity ligation assays (PLAs) and multicolor fluorescence microscopy, single RNA-protein interactions can be detected in fixed tissue or post live-cell hybridization, fixed ..
  14. Jeffrey R Schelling; Fiscal Year: 2016
    ..of NHE1, competition of LC- CoA for PI(4,5)P2 binding to NHE1 will be determined in vitro using fluorescence microscopy techniques in cells, phospholipids overlays and displacement of fluorescently labeled PI(4,5)P2 bound to ..
  15. SIGNAL TRANSDUCTION MECHANISMS OF ERYTHROPOIETIN
    Barbara A Miller; Fiscal Year: 2011
    ..Using quantitative fluorescence microscopy and electrophysiology, our laboratory was the first to demonstrate that Epo modulates the intracellular ..
  16. Live cell imaging of IgH and c-Myc gene loci and the role of nuclear organization
    Mark T Groudine; Fiscal Year: 2010
    ..translocations, the IgH and c-Myc loci will be imaged in live cells using 4D (3 dimensions over time) fluorescence microscopy. A major challenge in the field of nuclear structure and function has been to uncover the mechanisms ..
  17. Kafait U Malik; Fiscal Year: 2016
    ..BP and echocardiography for assessing cardiac function;4) histological, immunohistochemical, and fluorescence microscopy and biochemical techniques;5) flow cytometry to determine immune cell population in the blood and tissues;..
  18. Hypothermia, anesthesia and NMR metabolomics in ischemic neonatal brain slices
    Lawrence Litt; Fiscal Year: 2012
    ..Aim #3 will assess associations between tissue injury, quantifications of superoxide with ethidium fluorescence microscopy, and metabolomics, in situations where superoxide production is or is not attenuated by any of three ..
  19. CYCLE REGULATION IN THE SYMBIONT SINORHIZOBIUM MELILOTI
    KATHERINE ELISABETH GIBSON; Fiscal Year: 2011
    ..We are performing fluorescence microscopy to assess the effect HK mutations have on SmDivK-GFP localization as a measure of their potential impact ..
  20. The Effect Of Deafness Associated Mutations on MyosinVIIA Function
    Eva Forgacs; Fiscal Year: 2010
    ..added to the myosin VIIA and the movement of the molecule will be measured by Total Internal Reflection Fluorescence microscopy (TIRF)...
  21. Kim Janda; Fiscal Year: 2014
    ..To then validate mammalian cell receptor(s) of C12, a combination of fluorescence microscopy, RNAi and functional biochemical assays, or alternatively, protein overexpression, will be used...
  22. Nicholas Ferrell; Fiscal Year: 2016
    ..from sub-physiological to super- physiological and cytoskeletal reorganization will be characterized by fluorescence microscopy. Cellular uptake and intracellular trafficking of albumin will be characterized under flow and static ..
  23. Thomas Tuschl; Fiscal Year: 2015
    ..fields as genome and RNA sequencing, oligonucleotide probe design, automation of tissue processing, and fluorescence microscopy scanning and image processing, now position RNA ISH as a technology to rapidly surpass conventional IHC...
  24. Cardiomyocyte Zinc and its Regulation of Contraction-Relaxation Function
    Bradley M Palmer; Fiscal Year: 2012
    ..We will use conventional fluorescence microscopy to measure SR Ca2+ load and the rate of Na+-dependent Ca2+ efflux in isolated cardiomyocytes exposed to ..
  25. Neil Kaplowitz; Fiscal Year: 2016
    ..and Tissue Imaging Core, which consists of two subcores: Microscopy Subcore, which provides confocal and fluorescence microscopy using various platforms, and Histology Subcore, which provides liver tissue slide preparation, routine ..
  26. Taras Y Nazarko; Fiscal Year: 2014
    ..We will knock out the Y. lipolytica ATG36 and ACB1 genes, and elucidate their roles in lipophagy using fluorescence microscopy and a novel Tgl3-GFP processing assay...
  27. Jun Zhu; Fiscal Year: 2014
    ..the VIF levels seen in vivo to monitor and model virulence gene expression patterns using qRT-PCR and fluorescence microscopy. By understanding the properties and role of VIFs, we hope to overcome the greatest stumbling block in ..
  28. Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative Stress
    Stuart A Meyers; Fiscal Year: 2010
    ..level which includes membrane, cell signaling, and subcellular assessment of sperm function using fluorescence microscopy, flow cytometry, cryomicroscopy, and proteomics methods...
  29. Activation of PI Specific Phospholipase C Enzymes
    MARY FEDARKO ROBERTS; Fiscal Year: 2013
    ..sites using NMR and HXMS methods;and (iii) extend PI-PLC binding studies, using FCS and single molecule fluorescence microscopy, from simple vesicles to more complex target cell-like membranes...
  30. Tumor -Targeting Chemotherapeutic Agents
    Iwao Ojima; Fiscal Year: 2013
    ..tumor-targeting process and drug release in vitro and in vivo will be investigated by means of confocal fluorescence microscopy, MRI and PET...
  31. Novel Approach to Oral Gene Therapy for Chronic Granulomatous Disease
    Peter E Newburger; Fiscal Year: 2010
    ..Measurements will include fluorescence microscopy for particle uptake;molecular, histochemical and flow cytometric assays of gene expression and peroxide ..
  32. Genetic Interactions and Synthetic Population Biology
    Michael B Elowitz; Fiscal Year: 2013
    ..of the genotype distribution within a genetically diverse population using high-throughput single-cell fluorescence microscopy. (3) We shall use this synthetic system to test specific theoretical predictions concerning genetic ..
  33. Interactions between P. aeruginosa and autophagy in Airway epithelial cells
    INDHIRA H DE LA ROSA; Fiscal Year: 2013
    ..of T3SS effector proteins and by evaluating autophagy induction or blockage via Western blotting, fluorescence microscopy and co-Immunoprecipitation...
  34. Acquisition of a TIRF/Widefield fluorescence microscope for cell biology and neur
    Bryon D Grove; Fiscal Year: 2013
    ..imaging of dynamic molecular events at the cell membrane surface, and state of the art widefield fluorescence microscopy are necessary for investigators of the proposed projects to extend their studies...
  35. Analysis of the Spatiotemporal Regulation of Lipolysis
    Lisa M DiPilato; Fiscal Year: 2012
    ..to lipid droplets to monitor the effect of such perturbations on cAMP/PKA signaling in real time using fluorescence microscopy. Ultimately, I aim to address existing gaps in our understanding of the molecular mechanisms that are ..
  36. Functional genomics of human variation to Salmonella invasion
    DENNIS CHUN YONE KO; Fiscal Year: 2013
    ..typhi invasion. Fluorescence microscopy and flow cytometry will be used to determine if uptake of the bacteria or early establishment of an ..
  37. Excitation-contraction Coupling in Normal and Dystrophic Mammalian Muscle
    Julio L Vergara; Fiscal Year: 2013
    ..optical methods, such as F"ster resonance energy transfer (FRET) and total internal reflection fluorescence microscopy (TIRFM), to also assess the nanoscale localization of DGC and ECC proteins with respect to the internal ..
  38. Mannose Binding Lectin in Neuroinflammation and NeuroAIDS
    Kumud K Singh; Fiscal Year: 2013
    ..analyses during HIV infection, quantitative PCR validation, genotyping, immunohistostaining and fluorescence microscopy techniques...
  39. Chung Owyang; Fiscal Year: 2016
    ..by mass spectrometry and their intracellular locations determined by immunohistochemistry and confocal fluorescence microscopy. The purpose of the new Microbiome Core is to provide Center investigators access to state of the art ..
  40. Max A Cayo; Fiscal Year: 2016
    ..vectors encoding Oct4, Nanog, Sox2, Lin28) and validate the resulting iPSC by immunostaining/fluorescence microscopy, flow cytometry, DNA fingerprint STR analysis, karyotyping, and teratoma assay...
  41. Exocytosis and Coupled Endocytosis in Neuroendocrine Cells
    JOHN DAVID CASTLE; Fiscal Year: 2010
    ..phosphoinositide PIP2, required for exo-/endocytosis, will be evaluated using biophysical assays and fluorescence microscopy of cells deficient in SCAMPs or expressing SCAMP mutants defective in lipid sequestration...
  42. Frederick R Maxfield; Fiscal Year: 2014
    The basic cell biology of transport of lipids and proteins in cells will be studied using quantitative fluorescence microscopy coupled with biochemical and ultrastructural tools...
  43. Claudio D Stern; Fiscal Year: 2015
    ..the normal dynamics of segmentation and test them by quantitative measurements using advanced time-lapse fluorescence microscopy and microfluidics-based gradient-cell technology;2) use a novel experimental paradigm that allows ..
  44. Sandra L Schmid; Fiscal Year: 2016
    ..to track in an unbiased way every clathrin-coated structure (CCS) visible by total internal reflection fluorescence microscopy relative to actin cortex dynamics...
  45. JASON EDWARD LEE; Fiscal Year: 2015
    ..in non-invasive (MCF-7) and invasive (MDA-MB-231) breast cancer cells using live- cell and fixed-cell fluorescence microscopy. Furthermore, ER structure will be directly modulated in cells by overexpression or knockdown of ER ..
  46. Jason G Cyster; Fiscal Year: 2016
    ..One, we seek to further assess how SCS macrophages capture antigen and interact with B cells using fluorescence microscopy, flow cytometry and two-photon imaging approaches...
  47. Designing an Integrated Nanoscale System for Ion Channel Structure-Function Study
    Ratneshwar Lal; Fiscal Year: 2013
    ..Ion channel activity is analyzed by patch clamping and fluorescence microscopy. However, there is no integrated system for a direct 3D structure-activity study of these nano-..
  48. Susan Lowey; Fiscal Year: 2016
    ..Aim 1 will employ a combination of time-lapse epi-fluorescence microscopy and biochemical assays to assess the contribution of actin filament cross-linkers (fimbrin and transgelin)..
  49. Receptor Tyrosine Kinase Sorting and Transactivation in Endosomes
    MARK LINDSAY GRIMES; Fiscal Year: 2010
    ..Third, we will ask if ALK and TrkA are in the same or different endosomes in neuroblastoma cells using fluorescence microscopy and cell fractionation followed by immunoisolation of specific endosomes...
  50. Yale E Goldman; Fiscal Year: 2016
    ..We use an approach coupling Total Internal Reflection Fluorescence Microscopy (TIRFM) with single molecule Fluorescence Resonance Energy Transfer (smFRET)...
  51. Inhibition of chloride secretion by cigarette smoke
    JAMES LEVIN KREINDLER; Fiscal Year: 2010
    ..in the laboratory with exposure to electrophysiology, protein biochemistry, molecular biology, and fluorescence microscopy. Dr...
  52. Cytoskeletal Dynamics in Neuronal Dendrites
    Erik W Dent; Fiscal Year: 2013
    ..Using total internal reflection fluorescence microscopy (TIRFM), we show that MT invasion of dendritic spines can be associated with rapid morphological changes ..
  53. EGF Receptor Signaling in Time and Space in Tumor Cells
    Alexander D Sorkin; Fiscal Year: 2012
    ..proteins involved in ERK1/2 activation will then be analyzed in these new cell lines using quantitative fluorescence microscopy. Secondly, using a combination of RNA interference, live-cell fluorescence imaging, multi-chromophore ..
  54. Christoph J Fahrni; Fiscal Year: 2016
    ..biologists to visualize cellular copper by combining two powerful imaging modalities, light and X-ray fluorescence microscopy. The former approach entails the development of membrane diffusible high-contrast- ratio copper-..
  55. ERIC E ALANI; Fiscal Year: 2016
    ..In Aim 1 we are analyzing the behavior of single Mlh complexes interacting with DNA using total internal fluorescence microscopy. We are interested in answering two critical questions: 1...
  56. Release and trafficking of JC polyomavirus genomes and implications in AIDS
    CHRISTIAN DOUGLAS STUART NELSON; Fiscal Year: 2012
    ..infection will be performed in relation to fluorescently labeled cell markers by live cell confocal fluorescence microscopy. The second aim will investigate the nuclear import of JCV through the nuclear pore complex (NPC), ..
  57. Role of p190RhoGEF (Rgnef) in cell motility and tumor progression
    Nichol L G Miller; Fiscal Year: 2013
    ..Experiments will include RhoA activity assays, fluorescence microscopy monitoring of FA formation, and real-time imaging to evaluate Rgnef localization and the enhancement of ..
  58. Barbara A Baird; Fiscal Year: 2014
    ..signaling assemblies on the nanoscale with scanning electron microscopy and super high-resolution fluorescence microscopy, and apertures for near-field optical microscopy will be nanofabricated as a novel approach for measuring ..
  59. Duk Su Koh; Fiscal Year: 2015
    ..Tracking the SV in real-time with fluorescence microscopy will reveal how PAR-2 activation controls the SV in the cytoplasm and near the plasma membrane...
  60. Structural and Dynamical Response of Escherichia coli to Osmotic Stress
    James C Weisshaar; Fiscal Year: 2010
    ..diffusion) and the organization of proteins, DNA, and ribosomes within the cytoplasm of live cells using fluorescence microscopy. To create a sudden osmotic stress, we subject E...
  61. The structural role of ING proteins in chromatin remodeling.
    KAREN CHAMPAGNE GLASS; Fiscal Year: 2010
    ..significance of these mutations will also be tested in vivo by chromatin immunoprecipitation and fluorescence microscopy. These data will provide critical structural insights into the molecular mechanism used to discriminate ..
  62. Bal L Lokeshwar; Fiscal Year: 2015
    ..mutagenesis, chemical-cross linking, co-immunoprecipitation, confocal microscopy and time- resolved fluorescence microscopy. Specific Aim 2 is to determine the role of CXCR7 in tumor cell motility, invasion and enhancement of ..
  63. Sub-Wavelength Imaging of Intracellular Metal Ions
    Joseph R Lakowicz; Fiscal Year: 2010
    ..b>Fluorescence microscopy is one of the most widely used methods in the biological sciences...
  64. FLUORESCENT AND LARGE METAL CLUSTER COMBINATION PROBES
    Richard Powell; Fiscal Year: 1999
    ..These unique probes are to be developed for dual use in electron and fluorescence microscopy. Several new probes are proposed for the development during Phase I: 1) large platinum and palladium ..
  65. MELK as Functional Marker for Mammary Tumors
    Alexey Terskikh; Fiscal Year: 2009
    ..into cleared mammary fat pads;3) analyze tumors formed in transplanted animals using a combination of fluorescence microscopy, FACS and Q- PCR for the presence of MELK shRNA (mCherry/red) infected cells and endogenous MELK ..
  66. Olympus NanoZoomer RS Whole Slide Imaging System
    Brendan F Boyce; Fiscal Year: 2010
    ..by applicant): We propose to purchase an Olympus NanoZoomer Digital Pathology slide imaging system with a fluorescence microscopy attachment to support at least 26 NIH funded investigators at the University of Rochester Medical Center...
  67. RESISTANCE ARTERY REMODELING HYPERTENSION
    Russell Prewitt; Fiscal Year: 2005
    ..Confocal fluorescence microscopy will be used to locate the site of active forms of FAK and Src...
  68. High Performance Fluorescence Microscopy Imaging
    RAIMUND OBER; Fiscal Year: 2003
    ..as yet very limited understanding of intracellular trafficking of IgGs, and its dependence on FcRn using fluorescence microscopy techniques...