cryoelectron microscopy

Summary

Summary: Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.

Top Publications

  1. pmc Molecular architecture of native HIV-1 gp120 trimers
    Jun Liu
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Nature 455:109-13. 2008
  2. ncbi Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy
    Peter B Rosenthal
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 333:721-45. 2003
  3. pmc A Bayesian view on cryo-EM structure determination
    Sjors H W Scheres
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    J Mol Biol 415:406-18. 2012
  4. pmc Flexible fitting of atomic structures into electron microscopy maps using molecular dynamics
    Leonardo G Trabuco
    Beckman Institute, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Structure 16:673-83. 2008
  5. ncbi Distribution and three-dimensional structure of AIDS virus envelope spikes
    Ping Zhu
    Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306, USA
    Nature 441:847-52. 2006
  6. pmc Beam-induced motion of vitrified specimen on holey carbon film
    Axel F Brilot
    Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, MS029, 415 South Street, Waltham, MA 02454, USA
    J Struct Biol 177:630-7. 2012
  7. pmc Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach
    Keren Lasker
    Department of Bioengineering and Therapeutic Sciences, California Institute of Quantitative Biosciences, 1700 4th Street, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 109:1380-7. 2012
  8. pmc Appion: an integrated, database-driven pipeline to facilitate EM image processing
    Gabriel C Lander
    National Resource for Automated Molecular Microscopy, The Scripps Research Institute, CB 129, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    J Struct Biol 166:95-102. 2009
  9. ncbi The molecular architecture of axonemes revealed by cryoelectron tomography
    Daniela Nicastro
    Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, CB 347, University of Colorado, Boulder, CO 80309 0347, USA
    Science 313:944-8. 2006
  10. pmc Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution
    Jean Paul Armache
    Gene Center, Department of Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 107:19748-53. 2010

Research Grants

  1. 3D ELECTRON MICROSCOPY OF PYRUVATE DEHYDROGENASE
    JAMES STOOPS; Fiscal Year: 2002
  2. Present Homologous and Heterologous Antigen with Hepatitis E Virus
    R Holland Cheng; Fiscal Year: 2012
  3. Structure and function of Helicobacter pylori VacA
    Timothy L Cover; Fiscal Year: 2013
  4. Mechanisms of assembly of photoreceptor G protein complexes
    Barry M Willardson; Fiscal Year: 2013
  5. MOLECULAR BASIS OF ALPHAVIRUS ASSEMBLY
    Richard J Kuhn; Fiscal Year: 2013
  6. Circadian Programs in Bacteria
    Carl Hirschie Johnson; Fiscal Year: 2013
  7. Mechanisms of Viral DNA Packaging
    CARLOS JOSE BUSTAMANTE; Fiscal Year: 2013
  8. Functional Amyloid Formation in Streptococcus mutans
    L Jeannine Brady; Fiscal Year: 2013
  9. NEUTROPHIL FLAVOCYTOCHROME B STRUCTURE AND FUNCTION
    ALGIRDAS JOSEPH JESAITIS; Fiscal Year: 2010
  10. Structural Studies of Alphavirus Membrane Fusion In Situ
    Wei Zhang; Fiscal Year: 2010

Detail Information

Publications384 found, 100 shown here

  1. pmc Molecular architecture of native HIV-1 gp120 trimers
    Jun Liu
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Nature 455:109-13. 2008
    ..Our findings elucidate the structure and conformational changes of trimeric HIV-1 gp120 relevant to antibody neutralization and attachment to target cells...
  2. ncbi Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy
    Peter B Rosenthal
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Mol Biol 333:721-45. 2003
    ..7A resolution, according to a new objective criterion for resolution assessment proposed here...
  3. pmc A Bayesian view on cryo-EM structure determination
    Sjors H W Scheres
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    J Mol Biol 415:406-18. 2012
    ..Application to experimental data indicates that the statistical approach yields more reliable structures than existing methods and is capable of detecting smaller classes in data sets that contain multiple different structures...
  4. pmc Flexible fitting of atomic structures into electron microscopy maps using molecular dynamics
    Leonardo G Trabuco
    Beckman Institute, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Structure 16:673-83. 2008
    ..The MDFF method is then used to obtain high-resolution structures of the E. coli ribosome in different functional states imaged by cryo-EM...
  5. ncbi Distribution and three-dimensional structure of AIDS virus envelope spikes
    Ping Zhu
    Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306, USA
    Nature 441:847-52. 2006
    ..We applied cryoelectron microscopy tomography to define ultrastructural details of spikes...
  6. pmc Beam-induced motion of vitrified specimen on holey carbon film
    Axel F Brilot
    Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, MS029, 415 South Street, Waltham, MA 02454, USA
    J Struct Biol 177:630-7. 2012
    ..We show how alignment and averaging of movie frames can be used to restore high-resolution detail in images affected by beam-induced motion...
  7. pmc Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach
    Keren Lasker
    Department of Bioengineering and Therapeutic Sciences, California Institute of Quantitative Biosciences, 1700 4th Street, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 109:1380-7. 2012
    ..molecular architecture of the 26S holocomplex determined by an integrative approach based on data from cryoelectron microscopy, X-ray crystallography, residue-specific chemical cross-linking, and several proteomics techniques...
  8. pmc Appion: an integrated, database-driven pipeline to facilitate EM image processing
    Gabriel C Lander
    National Resource for Automated Molecular Microscopy, The Scripps Research Institute, CB 129, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    J Struct Biol 166:95-102. 2009
    ..Presented here is a description of the design and architecture of the working Appion pipeline prototype and some results of its use...
  9. ncbi The molecular architecture of axonemes revealed by cryoelectron tomography
    Daniela Nicastro
    Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, CB 347, University of Colorado, Boulder, CO 80309 0347, USA
    Science 313:944-8. 2006
    ..Periodic densities were also observed inside doublet microtubules; these may contribute to doublet stability...
  10. pmc Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution
    Jean Paul Armache
    Gene Center, Department of Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 107:19748-53. 2010
    ..Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum ..
  11. ncbi SPARX, a new environment for Cryo-EM image processing
    Michael Hohn
    Lawrence Berkeley National Laboratory, Physical Bioscience Division, Berkeley, CA 94720, USA
    J Struct Biol 157:47-55. 2007
    ..SPARX and all dependencies are free for academic use and available with complete source...
  12. pmc Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks
    Hongrong Liu
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, CA 90095 7364, USA
    Science 329:1038-43. 2010
    ..The third, mediated by IIIa and VIII, binds each GOS to five surrounding GONs. Optimization of adenovirus for cancer and gene therapy could target these networks...
  13. pmc The dynein regulatory complex is the nexin link and a major regulatory node in cilia and flagella
    Thomas Heuser
    Biology Department, Brandeis University, Waltham, MA 02453, USA
    J Cell Biol 187:921-33. 2009
    ..We further demonstrate that the DRC is the nexin link, which is thought to be critical for the generation of axonemal bending...
  14. ncbi The stoichiometry of Gag protein in HIV-1
    John A G Briggs
    Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7BN, UK
    Nat Struct Mol Biol 11:672-5. 2004
    ..In the immature virus, Gag forms a hexameric lattice with a spacing of 8.0 nm. Thus, less than half of the CA proteins form the mature core...
  15. pmc Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions
    Grigore D Pintilie
    Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA
    J Struct Biol 170:427-38. 2010
    ..We describe both interactive and automated ways of using these two methods. Finally, we show segmentation and fitting results for several experimentally-obtained density maps...
  16. ncbi Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins
    Shee Mei Lok
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Struct Mol Biol 15:312-7. 2008
    ..The changes in the structure of the viral surface are presumably responsible for inhibiting attachment to cells...
  17. pmc Mechanism of gate opening in the 20S proteasome by the proteasomal ATPases
    Julius Rabl
    Fachbereich Biologie, Chemie, Pharmazie, Freie Universitat Berlin, Takustrasse 3, 14195 Berlin, Germany
    Mol Cell 30:360-8. 2008
    ..This mechanism differs from that of PA26/28, which lacks the HbYX motif and does not cause alpha subunit rotation. These findings demonstrated how the ATPases' C termini function to facilitate substrate entry...
  18. pmc 3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry
    Xing Zhang
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095 7364, USA
    Cell 141:472-82. 2010
    ..These results suggest a well-orchestrated process of nonenveloped virus entry involving autocleavage of the penetration protein prior to exposure of its membrane-insertion finger...
  19. ncbi Structural basis of highly conserved ribosome recycling in eukaryotes and archaea
    Thomas Becker
    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, University of Munich, Feodor Lynen Straße 25, 81377 Munich, Germany
    Nature 482:501-6. 2012
    ..Using the mechanochemical properties of ABCE1, a conserved mechanism in archaea and eukaryotes is suggested that couples translation termination to recycling, and eventually to re-initiation...
  20. pmc Mechanism of folding chamber closure in a group II chaperonin
    Junjie Zhang
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 463:379-83. 2010
    ....
  21. ncbi Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy
    Niels Fischer
    3D Electron Cryomicroscopy Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nature 466:329-33. 2010
    ..The ribosome functions as a Brownian machine that couples spontaneous conformational changes driven by thermal energy to directed movement...
  22. ncbi Subnanometre-resolution structure of the intact Thermus thermophilus H+-driven ATP synthase
    Wilson C Y Lau
    Molecular Structure and Function Program, The Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Nature 481:214-8. 2012
    ..This structure therefore suggests the mechanism by which a transmembrane proton motive force is converted to rotation in rotary ATPases...
  23. pmc Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography
    Friedrich Förster
    Abteilung für Molekulare Strukturbiologie, Max Planck Institut fur Biochemie, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Proc Natl Acad Sci U S A 102:4729-34. 2005
    ..This study thus provides 3D structural information regarding the prefusion conformation of an intact unstained retrovirus surface protein...
  24. ncbi Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK
    Arash Komeili
    Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125, USA
    Science 311:242-5. 2006
    ..Thus, it seems that prokaryotes can use cytoskeletal filaments to position organelles within the cell...
  25. ncbi Microtubule structure at 8 A resolution
    Huilin Li
    Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA
    Structure 10:1317-28. 2002
    We have obtained a 3D reconstruction of intact microtubules, using cryoelectron microscopy and image processing, at a resolution of about 8 A, sufficient to resolve much of the secondary structure...
  26. pmc Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction
    Xing Zhang
    Department of Biochemistry and Howard Hughes Medical Institute, Brandeis University, MS029, 415 South Street, Waltham, MA 02454, USA
    Proc Natl Acad Sci U S A 105:1867-72. 2008
    ..This potential has broad implications for structural cell biology...
  27. ncbi Structural studies by electron tomography: from cells to molecules
    Vladan Lucic
    Department of Structural Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    Annu Rev Biochem 74:833-65. 2005
    ....
  28. pmc Analysis of cryo-electron microscopy images does not support the existence of 30-nm chromatin fibers in mitotic chromosomes in situ
    Mikhail Eltsov
    Laboratoire d Analyse Ultrastructurale, Universite de Lausanne, Biophore, CH 1015, Lausanne, Switzerland
    Proc Natl Acad Sci U S A 105:19732-7. 2008
    ....
  29. pmc Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome
    Jean Paul Armache
    Gene Center, Department of Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 107:19754-9. 2010
    ..Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5...
  30. ncbi Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution
    Daniel Studer
    Institute for Anatomy, University of Bern, Baltzerstr 2, 3000, Bern 9, Switzerland
    Histochem Cell Biol 130:877-89. 2008
    ..High pressure freezing of tissue is the base which may lead to precise models of macromolecular assemblies in situ, and thus to a better understanding of the function of complex cellular structures...
  31. pmc Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures
    Audray Harris
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:11440-5. 2011
    ....
  32. pmc Conformational changes of the flavivirus E glycoprotein
    Ying Zhang
    Department of Biological Sciences, Lilly Hall, 915 West State Street, Purdue University, West Lafayette, IN 47907, USA
    Structure 12:1607-18. 2004
    ..Flexibility of E is apparently a functional requirement for assembly and infection of flaviviruses...
  33. ncbi Structural basis of pore formation by the bacterial toxin pneumolysin
    Sarah J Tilley
    School of Crystallography and Institute of Structural Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom
    Cell 121:247-56. 2005
    ..With an oligomer size of up to 44 subunits in the pore, this assembly creates a transmembrane channel 260 A in diameter lined by 176 beta strands...
  34. pmc Structural basis for the function of a small GTPase RsgA on the 30S ribosomal subunit maturation revealed by cryoelectron microscopy
    Qiang Guo
    Ministry of Education Protein Science Laboratory, Center for Structural Biology, School of Life Sciences, and Department of Chemistry, Tsinghua University, Beijing 100084, China
    Proc Natl Acad Sci U S A 108:13100-5. 2011
    ..Using cryoelectron microscopy, we have determined the structure of the 30S subunit bound with RsgA in the presence of GMPPNP at ..
  35. pmc Structural characterization of mRNA-tRNA translocation intermediates
    Xabier Agirrezabala
    Structural Biology Unit, CIC bioGUNE, Bizkaia Technology Park, Derio 48160, Basque Country, Spain
    Proc Natl Acad Sci U S A 109:6094-9. 2012
    ..The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state via a series of substates with incremental changes in conformation...
  36. ncbi Molecular model for a complete clathrin lattice from electron cryomicroscopy
    Alexander Fotin
    Biophysics Graduate Program, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:573-9. 2004
    ..These invariant local interactions appear to stabilize the lattice, allowing assembly and uncoating to be controlled by events at a few specific sites...
  37. pmc Understanding ribosome assembly: the structure of in vivo assembled immature 30S subunits revealed by cryo-electron microscopy
    Ahmad Jomaa
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8N3Z5, Canada
    RNA 17:697-709. 2011
    ..In addition, we demonstrate how studies of emerging assembly factors in ribosome biogenesis can help to elucidate the path of subunit assembly in vivo...
  38. pmc Template-free 13-protofilament microtubule-MAP assembly visualized at 8 A resolution
    Franck J Fourniol
    Institute of Structural and Molecular Biology, Birkbeck College, London, England, UK
    J Cell Biol 191:463-70. 2010
    ..DCX's exquisite binding selectivity uncovers important insights into regulation of cellular MTs...
  39. ncbi Structural basis for the regulated protease and chaperone function of DegP
    Tobias Krojer
    Research Institute for Molecular Pathology IMP, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Nature 453:885-90. 2008
    ..Oligomer reassembly and concomitant activation on substrate binding may also be critical in regulating other HtrA proteases implicated in protein-folding diseases...
  40. pmc Single-particle cryoelectron microscopy analysis reveals the HIV-1 spike as a tripod structure
    Shang Rung Wu
    Department of Biosciences and Nutrition, Karolinska Institute, S 141 83 Huddinge, Sweden
    Proc Natl Acad Sci U S A 107:18844-9. 2010
    ..We solubilized and isolated unliganded and CD4-bound spikes from virus-like particles and used cryoelectron microscopy to reconstruct their 3D structures...
  41. pmc Structure of dengue virus: implications for flavivirus organization, maturation, and fusion
    Richard J Kuhn
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Cell 108:717-25. 2002
    The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map...
  42. pmc Direct visualization of the outer membrane of mycobacteria and corynebacteria in their native state
    Benoit Zuber
    Laboratory of Ultrastructural Analysis, University of Lausanne, Biophore Building, 1015 Lausanne, Switzerland
    J Bacteriol 190:5672-80. 2008
    ..The architecture we describe should serve as a reference for future studies to relate the structure of the mycobacterial cell envelope to its function...
  43. pmc Tilt-pair analysis of images from a range of different specimens in single-particle electron cryomicroscopy
    Richard Henderson
    MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    J Mol Biol 413:1028-46. 2011
    ..A convincing tilt-pair parameter plot, where 60% of the particles show a single cluster around the expected tilt axis and tilt angle, provides confidence in a structure determined using electron cryomicroscopy...
  44. pmc Structural insight into nascent polypeptide chain-mediated translational stalling
    Birgit Seidelt
    Gene Center and Center for Integrated Protein Science Munich CIPSM, Department for Chemistry and Biochemistry, University of Munich, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Science 326:1412-5. 2009
    ..We propose a model whereby interactions within the tunnel are relayed to the peptidyltransferase center to inhibit translation. Moreover, we show that nascent chains adopt distinct conformations within the ribosomal exit tunnel...
  45. pmc Refinement of protein structures into low-resolution density maps using rosetta
    Frank DiMaio
    Department of Biochemistry, University of Washington, Seattle, 98195, USA
    J Mol Biol 392:181-90. 2009
    ..The method can achieve near-atomic resolution starting from density maps at 4-6 A resolution...
  46. pmc Structural organization of a filamentous influenza A virus
    Lesley J Calder
    Division of Physical Biochemistry, MRC National Institute for Medical Research, London, UK
    Proc Natl Acad Sci U S A 107:10685-90. 2010
    ..The polar organization of the virus provides a model for assembly of the virion during budding at the host membrane. Images and tomograms of A/Aichi/68 X-31 virions show the generality of these conclusions to non-filamentous virions...
  47. pmc Computational resources for cryo-electron tomography in Bsoft
    J Bernard Heymann
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Building 50, Room 1515, 50 South Drive MSC 8025, Bethesda, MD 20892 8025, USA
    J Struct Biol 161:232-42. 2008
    ..Resources are also included that allow resolution assessment by cross-validation (NLOO2D); denoising and interpretation; and the extraction, mutual alignment, and averaging of tomographic sub-volumes...
  48. pmc Molecular dynamics flexible fitting: a practical guide to combine cryo-electron microscopy and X-ray crystallography
    Leonardo G Trabuco
    Beckman Institute for Advanced Science and Technology, Urbana, IL 61801, USA
    Methods 49:174-80. 2009
    ..In this paper we describe the necessary steps to set up, run, and analyze MDFF simulations and the software packages that implement the corresponding functionalities...
  49. pmc Electron cryotomography of Tula hantavirus suggests a unique assembly paradigm for enveloped viruses
    Juha T Huiskonen
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Virol 84:4889-97. 2010
    ..These interactions may be sufficient for creating membrane curvature during virus budding. In conclusion, the structure and assembly principles of Tula hantavirus exemplify a unique assembly paradigm for enveloped viruses...
  50. ncbi Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus
    Rajiv G Tawar
    Institut Pasteur, Unité de Virologie Structurale, Département de Virologie and CNRS Unité de Recherche Associée URA 3015, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Science 326:1279-83. 2009
    ..Furthermore, the nucleoprotein structure highlights possible key sites for drug targeting...
  51. pmc Cryo-electron microscopy of vitreous sections
    Ashraf Al-Amoudi
    Laboratoire d Analyse Ultrastructurale, Batiment de Biologie, Universite de Lausanne, Lausanne, Switzerland
    EMBO J 23:3583-8. 2004
    ..CEMOVIS will give its full potential when combined with computerized electron tomography for 3-d reconstruction...
  52. ncbi Finding rigid bodies in protein structures: Application to flexible fitting into cryoEM maps
    Arun Prasad Pandurangan
    Institute of Structural and Molecular Biology, Crystallography Department of Biological Sciences, Birkbeck College, University of London, London WC1E 7HX, United Kingdom
    J Struct Biol 177:520-31. 2012
    ..The method is stand-alone and could serve as a general interactive tool for guiding flexible fitting into EM maps...
  53. ncbi A visual approach to proteomics
    Stephan Nickell
    Max Planck Institute for Biochemistry, Department of Structural Biology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Rev Mol Cell Biol 7:225-30. 2006
    ..Visual proteomics' aims to complement and extend mass-spectrometry-based inventories, and to provide a quantitative description of the macromolecular interactions that underlie cellular functions...
  54. pmc Influenza virus pleiomorphy characterized by cryoelectron tomography
    Audray Harris
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:19123-7. 2006
    ....
  55. pmc Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography
    Alexander Rigort
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Proc Natl Acad Sci U S A 109:4449-54. 2012
    ..We illustrate the quality of sample preservation with a structure of the nuclear pore complex obtained from a single tomogram...
  56. pmc High-resolution cryo-electron microscopy structures of murine norovirus 1 and rabbit hemorrhagic disease virus reveal marked flexibility in the receptor binding domains
    Umesh Katpally
    Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO 63132, USA
    J Virol 84:5836-41. 2010
    ..Connections between the P and shell domains and between the floating P domains were modeled. This observed P-domain flexibility likely facilitates virus-host receptor interactions...
  57. pmc Poliovirus RNA is released from the capsid near a twofold symmetry axis
    Mihnea Bostina
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Virol 85:776-83. 2011
    ....
  58. pmc Protein structure fitting and refinement guided by cryo-EM density
    Maya Topf
    School of Crystallography, Birkbeck College, University of London, Malet Street, London WC1E 7HX, United Kingdom
    Structure 16:295-307. 2008
    ..At approximately 10 A resolution, Calpha rmsd between the initial and final structures was reduced on average by approximately 53%. The method is automated and can refine both experimental and predicted atomic structures...
  59. ncbi Ribosome binding of a single copy of the SecY complex: implications for protein translocation
    Jean François Ménétret
    Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118 2526, USA
    Mol Cell 28:1083-92. 2007
    ..We also show that point mutations of basic residues within either loop abolish ribosome binding. We suggest that SecY binds to this primary site on the ribosome and subsequently captures and translocates the nascent chain...
  60. pmc Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd
    Thomas G Wendt
    European Molecular Biology Laboratory, Structure Programme, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO J 21:5969-78. 2002
    ....
  61. pmc Structural insights into the assembly of the type III secretion needle complex
    Thomas C Marlovits
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520 8024, USA
    Science 306:1040-2. 2004
    ..Moreover, conformational changes during needle assembly created scaffolds for anchoring both PrgJ and the needle substructure and may provide the basis for substrate-specificity switching during type III secretion...
  62. pmc Cryo-electron microscopy structure of an adenovirus-integrin complex indicates conformational changes in both penton base and integrin
    Steffen Lindert
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 2215 Garland Avenue, Nashville, TN 37232, USA
    J Virol 83:11491-501. 2009
    ..These findings shed further light on the structural basis of integrin binding to biologically relevant ligands, as well as on the molecular events leading to HAdV cell entry...
  63. pmc Modeling experimental image formation for likelihood-based classification of electron microscopy data
    Sjors H W Scheres
    Centro Nacional de Biotecnologia CSIC, Cantoblanco, 28049, Madrid, Spain
    Structure 15:1167-77. 2007
    ..Their effectiveness is demonstrated with various examples, including 2D classification of top views of the archaeal helicase MCM and 3D classification of 70S E. coli ribosome and Simian Virus 40 large T-antigen projections...
  64. ncbi Three-dimensional structure of herpes simplex virus from cryo-electron tomography
    Kay Grünewald
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 302:1396-8. 2003
    ..The envelope contained 600 to 750 glycoprotein spikes that varied in length, spacing, and in the angles at which they emerge from the membrane. Their distribution was nonrandom, suggesting functional clustering...
  65. pmc Dynamic reorganization of the functionally active ribosome explored by normal mode analysis and cryo-electron microscopy
    Florence Tama
    Department of Molecular Biology TPC6, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 100:9319-23. 2003
    ....
  66. ncbi Nucleotide-induced global conformational changes of flagellar dynein arms revealed by in situ analysis
    Tandis Movassagh
    Department of Biology, ETH Zurich, Zurich, Switzerland
    Nat Struct Mol Biol 17:761-7. 2010
    ..In contrast to in vitro results, in the presence of nucleotides, outer dynein arms coexist as clusters of apo or nucleotide-bound forms in situ. This implies a cooperative switching, which may be related to the mechanism of bending...
  67. ncbi The molecular sociology of the cell
    Carol V Robinson
    Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge CB2 1EW, UK
    Nature 450:973-82. 2007
    ..With these structures in hand, researchers will be poised to use cryo-electron tomography to view protein complexes in action within cells, providing unprecedented insights into protein-interaction networks...
  68. pmc Adding the third dimension to virus life cycles: three-dimensional reconstruction of icosahedral viruses from cryo-electron micrographs
    T S Baker
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
    Microbiol Mol Biol Rev 63:862-922, table of contents. 1999
    ....
  69. ncbi Maximum-likelihood multi-reference refinement for electron microscopy images
    Sjors H W Scheres
    Biocomputing Unit, Centro Nacional de Biotecnologia, Campus Universidad Autonoma, Cantoblanco, 28049, Madrid, Spain
    J Mol Biol 348:139-49. 2005
    ..Results obtained using simulated data suggest that the efficiency of the maximum-likelihood approach may be further enhanced by explicitly incorporating the microscope contrast transfer function in the image formation model...
  70. ncbi Automated acquisition of cryo-electron micrographs for single particle reconstruction on an FEI Tecnai electron microscope
    Jianlin Lei
    Wadsworth Center, Empire State Plaza, Albany, NY 12201 0509, USA
    J Struct Biol 150:69-80. 2005
    ..A number of applications show that the performance of the program is satisfactory and the quality of the micrographs and their power spectra acquired by the program is comparable to those manually collected under the same conditions...
  71. pmc 4.0-A resolution cryo-EM structure of the mammalian chaperonin TRiC/CCT reveals its unique subunit arrangement
    Yao Cong
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemsitry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030
    Proc Natl Acad Sci U S A 107:4967-72. 2010
    ..These interior surface chemical properties likely play an important role in TRiC's cellular substrate specificity...
  72. pmc An atomic-level mechanism for activation of the kinesin molecular motors
    Charles V Sindelar
    Life Sciences Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 107:4111-6. 2010
    ..We used cryoelectron microscopy to derive 8-9 A-resolution maps of four nucleotide states encompassing the microtubule-attached kinetic ..
  73. pmc Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome
    Thomas Becker
    Gene Center Munich and Center for Integrated Protein Science, Department of Chemistry and Biochemistry, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, 81377 Munich, Germany
    Science 326:1369-73. 2009
    ..This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation...
  74. ncbi Visualization of proteins in intact cells with a clonable tag for electron microscopy
    Elia Diestra
    Cell Structure Lab, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, Darwin 3, Campus de Cantoblanco, Madrid 28049, Spain
    J Struct Biol 165:157-68. 2009
    ..The electron-dense tag was easily visualized by electron tomography and in frozen-hydrated cells...
  75. ncbi Sharpening high resolution information in single particle electron cryomicroscopy
    J J Fernandez
    Centro Nacional de Biotecnologia, CSIC Campus Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
    J Struct Biol 164:170-5. 2008
    ..Therefore, the method may facilitate the analysis of experimental data in high resolution single particle electron cryomicroscopy...
  76. ncbi Tools for correlative cryo-fluorescence microscopy and cryo-electron tomography applied to whole mitochondria in human endothelial cells
    Linda F van Driel
    Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, Leiden, The Netherlands
    Eur J Cell Biol 88:669-84. 2009
    ..Taken together, this study emphasizes that cryo-CLEM can be a routinely used technique that opens up exciting new possibilities for cryo-ET...
  77. pmc Zernike phase contrast cryo-electron microscopy and tomography for structure determination at nanometer and subnanometer resolutions
    Kazuyoshi Murata
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 18:903-12. 2010
    ..These results show quantitatively the benefits of ZPC-cryoEM and ZPC-cryoET for structural determinations of macromolecular machines at nanometer and subnanometer resolutions...
  78. pmc Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy
    Nikolaus Grigorieff
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02154, USA
    Curr Opin Struct Biol 21:265-73. 2011
    ..This review summarizes technical developments, both in instrumentation and in computation, that have led to the new structures, which advance our understanding of virus assembly and cell entry...
  79. pmc Cryo-electron tomography of microtubule-kinesin motor complexes
    Julia Cope
    The Boulder Laboratory for 3 D Microscopy of Cells, University of Colorado at Boulder, Department of Molecular, Cellular, and Developmental Biology, Boulder, CO 80309 0347, USA
    J Struct Biol 170:257-65. 2010
    ....
  80. pmc Large conformational changes in a kinesin motor catalyzed by interaction with microtubules
    Keiko Hirose
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8562, Japan
    Mol Cell 23:913-23. 2006
    ....
  81. pmc Structural characterization of the dual glycan binding adeno-associated virus serotype 6
    Robert Ng
    Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610 0266, USA
    J Virol 84:12945-57. 2010
    ....
  82. pmc Cryo-EM study of the spinach chloroplast ribosome reveals the structural and functional roles of plastid-specific ribosomal proteins
    Manjuli R Sharma
    Laboratory of Structural Pathology, Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201 0509, USA
    Proc Natl Acad Sci U S A 104:19315-20. 2007
    ..Our study provides evolutionary insights into the structural and functional roles that the PSRPs play during protein synthesis in chloroplasts...
  83. pmc Inferential optimization for simultaneous fitting of multiple components into a CryoEM map of their assembly
    Keren Lasker
    Blavatnik School of Computer Science, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    J Mol Biol 388:180-94. 2009
    ..A near-native configuration was usually found as the top-scoring model. Therefore, MultiFit can provide initial configurations for further refinement of many multicomponent assembly structures described by electron microscopy...
  84. pmc High-resolution x-ray structure and functional analysis of the murine norovirus 1 capsid protein protruding domain
    Stefan Taube
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    J Virol 84:5695-705. 2010
    ..Modeling of a Fab fragment and crystal structures of the P dimer into the cryoelectron microscopy three-dimensional (3D) image reconstruction of the A6...
  85. ncbi Localization of the ribosomal protection protein Tet(O) on the ribosome and the mechanism of tetracycline resistance
    C M Spahn
    Howard Hughes Medical Institute, Health Research Inc. at the Wadsworth Center, Empire State Plaza, Albany, NY 12201, USA
    Mol Cell 7:1037-45. 2001
    ..Our findings give insights into the mechanism of tetracycline resistance...
  86. ncbi Cryo-electron microscopy reconstructions of two types of wild rabbit hemorrhagic disease viruses characterized the structural features of Lagovirus
    Zhongjun Hu
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Protein Cell 1:48-58. 2010
    ..Collectively, our cryo-electron microscopic studies of RHDV provide close insight into the structure of Lagovirus, which is important for functional analysis and better vaccine development in the future...
  87. pmc Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor
    Ruiwu Wang
    Libin Cardiovascular Institute of Alberta, Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 286:12202-12. 2011
    ..6-binding site but distant to the central region around residue Ser-2367. An allosteric mechanism by which dantrolene stabilizes interdomain interactions between the NH2-terminal and central regions is proposed...
  88. pmc Cryo-EM structure of a group II chaperonin in the prehydrolysis ATP-bound state leading to lid closure
    Junjie Zhang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 19:633-9. 2011
    ..This mechanism of structural changes in response to ATP is entirely different from those found in group I chaperonins...
  89. ncbi Cryo-electron tomography: gaining insight into cellular processes by structural approaches
    Tal Yahav
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Curr Opin Struct Biol 21:670-7. 2011
    ..We discuss recent developments and structural findings enabling major strides to be made in understanding complex physiological functions...
  90. pmc Applications of the molecular dynamics flexible fitting method
    Leonardo G Trabuco
    Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Struct Biol 173:420-7. 2011
    ..In the present work, recent applications of MDFF are presented, including the interpretation of cryo-EM data of the ribosome at different stages of translation and the structure of a membrane-curvature-inducing photosynthetic complex...
  91. pmc Labeling and localization of the herpes simplex virus capsid protein UL25 and its interaction with the two triplexes closest to the penton
    James F Conway
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Mol Biol 397:575-86. 2010
    ..These results support the view that UL25 is present on incoming capsids with the capsid-binding domain of UL25 located on the surface of the mature DNA-containing capsid...
  92. pmc Subnanometer-resolution electron cryomicroscopy-based domain models for the cytoplasmic region of skeletal muscle RyR channel
    Irina I Serysheva
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:9610-5. 2008
    ..Taken together, these results provide a structural context for both channel gating and the consequences of certain malignant hyperthermia and central core disease-associated mutations in RyR1...
  93. ncbi Structure of the 30S translation initiation complex
    Angelita Simonetti
    Institute of Genetics and of Molecular and Cellular Biology, Department of Structural Biology and Genomics, Illkirch F 67404, France
    Nature 455:416-20. 2008
    ....
  94. pmc Crystal structure of group II chaperonin in the open state
    Yanwu Huo
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Structure 18:1270-9. 2010
    ..Structural comparison as well as protease K digestion indicated only ATP binding without hydrolysis does not induce chamber closure of thermosome...
  95. pmc Multiple states of a nucleotide-bound group 2 chaperonin
    Daniel K Clare
    Department of Crystallography, Birkbeck College and Institute of Structural Molecular Biology, University of London, London WC1E 7HX, United Kingdom
    Structure 16:528-34. 2008
    ....
  96. pmc Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites
    Andreas H Ratje
    Institut fur Medizinische Physik und Biophysik, Charite Universitatsmedizin Berlin, Ziegelstrasse 5 9, 10117 Berlin, Germany
    Nature 468:713-6. 2010
    ..Here we use multiparticle cryoelectron microscopy analysis to resolve two previously unseen subpopulations within Thermus thermophilus EF-G-ribosome ..
  97. pmc p53 activates transcription by directing structural shifts in Mediator
    Krista D Meyer
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado, USA
    Nat Struct Mol Biol 17:753-60. 2010
    ..Our results define a fundamental mechanism by which p53 activates transcription and suggest that Mediator structural shifts trigger activation of stalled Pol II complexes...
  98. pmc Topology and organization of the Salmonella typhimurium type III secretion needle complex components
    Oliver Schraidt
    Research Institute of Molecular Pathology, Vienna, Austria
    PLoS Pathog 6:e1000824. 2010
    ..This study provides insights into the organization of this evolutionary highly conserved nanomachinery and is the basis for further functional analysis...
  99. pmc Coordinated movement of cytoplasmic and transmembrane domains of RyR1 upon gating
    Montserrat Samso
    Division of Anesthesia Research, Department of Anesthesia, Perioperative and Pain Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Biol 7:e85. 2009
    ....
  100. pmc Crystal structure of CD155 and electron microscopic studies of its complexes with polioviruses
    Ping Zhang
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907 2054, USA
    Proc Natl Acad Sci U S A 105:18284-9. 2008
    ..5-A resolution and fitted into approximately 8.5-A resolution cryoelectron microscopy reconstructions of the virus-receptor complexes for the 3 PV serotypes...
  101. pmc Association of the pr peptides with dengue virus at acidic pH blocks membrane fusion
    I M Yu
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Virol 83:12101-7. 2009
    ..R. Chipman, R. J. Kuhn, M. G. Rossmann, and J. Chen, Science 319:1834-1837, 2008). Here we show, using cryoelectron microscopy, that the structure of immature dengue virus at pH 6...

Research Grants74

  1. 3D ELECTRON MICROSCOPY OF PYRUVATE DEHYDROGENASE
    JAMES STOOPS; Fiscal Year: 2002
    ..A 3-D reconstruction of the beef kidney intact PDC will permit a comparison of the mammalian and yeast complexes. ..
  2. Present Homologous and Heterologous Antigen with Hepatitis E Virus
    R Holland Cheng; Fiscal Year: 2012
    ....
  3. Structure and function of Helicobacter pylori VacA
    Timothy L Cover; Fiscal Year: 2013
    ..abstract_text> ..
  4. Mechanisms of assembly of photoreceptor G protein complexes
    Barry M Willardson; Fiscal Year: 2013
    ..An understanding of the way these protein complexes are brought together is necessary to develop treatments that would allow the complexes to assemble and function despite the mutations. ..
  5. MOLECULAR BASIS OF ALPHAVIRUS ASSEMBLY
    Richard J Kuhn; Fiscal Year: 2013
    ..This insight will enable us, and others, to pursue novel strategies for virus and disease intervention. ..
  6. Circadian Programs in Bacteria
    Carl Hirschie Johnson; Fiscal Year: 2013
    ..Knowledge of circadian mechanisms along with the development of therapies to properly phase sleep will allow us to enhance health, performance, and well-being in addition to improving the quality of life for depressed subjects. ..
  7. Mechanisms of Viral DNA Packaging
    CARLOS JOSE BUSTAMANTE; Fiscal Year: 2013
    ..Finally, we wish to investigate how the increasing internal DNA pressure regulates the dynamics of the motor during packaging. ..
  8. Functional Amyloid Formation in Streptococcus mutans
    L Jeannine Brady; Fiscal Year: 2013
    ..Together these Aims will reveal the contribution of amyloid formation to S. mutans biology, and demonstrate the feasibility of amyloid inhibition as a therapeutic approach to preventing biofilm formation by this pathogen. ..
  9. NEUTROPHIL FLAVOCYTOCHROME B STRUCTURE AND FUNCTION
    ALGIRDAS JOSEPH JESAITIS; Fiscal Year: 2010
    ..It also outlines a plan to analyze Cytb molecular shape by single molecule conventional and cryoelectron microscopy. Lastly, the proposal exploits recent progress made in antibody imprinting, an application of phage ..
  10. Structural Studies of Alphavirus Membrane Fusion In Situ
    Wei Zhang; Fiscal Year: 2010
    ..Knowledge gained from such studies provides potential targets for anti-viral reagents. ..
  11. Structure-function studies of alpha- and flaviviruses
    Richard J Kuhn; Fiscal Year: 2010
    ..abstract_text> ..
  12. Structural Mechanism of the Acetylcholine Receptor in Tubular Membrane Crystals
    PETER NIGEL UNWIN; Fiscal Year: 2013
    ..By understanding better the biological mechanisms of these receptors, we contribute basic insight into the nature of the disorders themselves. ..
  13. Structures of Lipid-DNA Nonviral Gene Delivery Systems 4
    Cyrus R Safinya; Fiscal Year: 2013
    ....
  14. Defining the cis and trans acting factors required for assembly of infectious Blu
    Polly Roy; Fiscal Year: 2013
    ..Completion of these goals will pave the way for the rational design of inhibitors to block virus replication and provide lead compounds for related viruses of pathogenic significance to man and animals. ..
  15. Protein Kinase Regulatory Subunit I Directed Mutagenesis
    Susan S Taylor; Fiscal Year: 2013
    ..The work will not only enhance our basic understanding of these molecular switches but will also open the door for the design of new therapeutics capable of interfering with cell signaling. ..
  16. Studies of metal-dependent intercellular adhesion in Staphylococcal biofilms
    Andrew B Herr; Fiscal Year: 2013
    ..These studies will lead to a better understanding of a major mechanism for biofilm formation and will help aid the development of inexpensive and broadly effective approaches for preventing biofilm formation. ..
  17. Architectural Basis of Leptin Transmembrane Signaling
    Georgios Skiniotis; Fiscal Year: 2013
    ....
  18. The Molecular Structure of the RNA Polymerase Portal of a Bacteriophage-Reovirus
    PAUL JEFFREY GOTTLIEB; Fiscal Year: 2013
    ..We anticipate that the optical qualities of viral proteins that we discover are applicable to human pathogens, in particular influenza. ..
  19. Synaptotagmin and C2-Domains: Structure and Function
    JOSE RIZO-REY; Fiscal Year: 2013
    ..Moreover, since many neurological disorders are treated with drugs that alter synaptic transmission, this research is expected to provide crucial clues for the development of novel strategies to understand and treat these disorders. ..
  20. High-throughput Pipeline for Electron Crystallography
    David L Stokes; Fiscal Year: 2013
    ..As a result, membrane proteins are implicated in many diseases. Structural information about these proteins is critical to understanding the biology behind the disease and for designing drugs to ameliorate the problems. ..
  21. Co-chaperone role of phosducin-like protein in G protein subunit assembly
    Barry M Willardson; Fiscal Year: 2010
    ....
  22. Structural and mechanistic studies of mitochondrial fission
    David C Chan; Fiscal Year: 2010
    ..These studies will lead to structural insights that will enable investigators to manipulate mitochondrial fission, and thereby potentially modulate processes such as apoptosis, cancer, and nerve function. ..
  23. A TG model for functional effects of FHC mutations in b-cardiac myosin
    Susan Lowey; Fiscal Year: 2013
    ..These studies should serve to increase our knowledge of the basic molecular mechanism of this disease by examining the effects of an FHC mutation at increasing levels of complexity from the molecule to the filament. ..
  24. Nanomedicine Center for Nucleoprotein Machines
    Gang Bao; Fiscal Year: 2013
    ..abstract_text> ..
  25. Structural/Functional Modularity in Nitric Oxide Synthase
    Linda J Roman; Fiscal Year: 2012
    ..abstract_text> ..
  26. Mechanisms in Polyoma and Papillomavirus Assembly
    ROBERT GARCEA; Fiscal Year: 2013
    ..abstract_text> ..
  27. Molecular biology of viral capsids
    CHENG C KAO; Fiscal Year: 2013
    ..two aims, the first will seek to understand the architecture of the virion through a combination of cryoelectron microscopy and single particle reconstruction, biochemical mapping of RNA conformation and RNA-capsid interaction, ..
  28. Structural mechanisms of Clostridium difficile pathogenesis
    DANA BORDEN LACY; Fiscal Year: 2013
    ..difficile a significant public health concern. The goal of the proposed project is to identify the structural and molecular mechanisms by which the two primary toxins, TcdA and TcdB, disrupt host cell function. ..
  29. Structural Studies of Telomerase Holoenzyme
    EDWARD JOHN MIRACCO; Fiscal Year: 2013
    ..The goal of this fellowship will be to acquire a cryoelectron microscopy structure of the Tetrahymena thermophila telomerase holoenzyme complex, assign each component, deduce ..
  30. Structural Basis of Dysfunctional Calcium Release Channels in Heart Disease
    Zheng Liu; Fiscal Year: 2013
    ....
  31. Poliovirus Cell Entry Pathways
    James M Hogle; Fiscal Year: 2012
    ..The project seeks to develop a hybrid approach combining x-ray crystallography, cryoelectron microscopy, cyroelectron tomography and optical microscopy to develop a series of structural snapshots of cell entry ..
  32. Electron Microscopy of P-type Ion Pumps
    David L Stokes; Fiscal Year: 2010
    ..We will use cryoelectron microscopy of two-dimensional crystals to determine structures and will interpret the resulting density maps by ..
  33. MECHANISMS OF SITE-SPECIFIC RECOMBINATION
    Arthur Landy; Fiscal Year: 2012
    ..These pathways are responsible for the ubiquitous spread of both pathogenicity and resistance to antibiotics. ..
  34. Structures of the portal vertex in dsDNA viruses and virus-infected cells
    Wah Chiu; Fiscal Year: 2011
    ..Our technical approaches and biological insights will be transformative in the study of viruses, not only in biochemically purified states, but also in their native cellular environments. ..
  35. Structural Aspects of Protein Synthesis in the Mammalian Mitochondria
    Rajendra K Agrawal; Fiscal Year: 2013
    ....
  36. Cellular attachment, penetration and transport of non-enveloped dsRNA viruses
    Z Hong Zhou; Fiscal Year: 2013
    ..These mechanisms and structures will be correlated with those of other enveloped and non-enveloped viruses, including the ones cited above. ..
  37. A Feeding Tube Model for Bacterial Cell-Cell Communication
    AMY HITCHCOCK CAMP; Fiscal Year: 2012
    ..subtilis such as B. anthracis and Clostridium difficile. ..
  38. ASSEMBLY AND TRANSFER OF N-LINKED OLIGOSACCHARIDES
    JAMES REID GILMORE; Fiscal Year: 2013
    ..of the OST, the Sec61 protein translocation channel and a translating ribosome will be prepared for cryoelectron microscopy. Structural analysis of these complexes should provide insight into the location of the STT3 active site ..
  39. HTLV-1 Particle Analysis and Gag Interactions
    Louis M Mansky; Fiscal Year: 2013
    ..The results from these proposed studies should provide further insight into fundamental aspects of HTLV-1 and retrovirus particle assembly, which may aid in developing therapeutics. ..
  40. Mass Spectrometry of Protein Assemblies
    Joseph A Loo; Fiscal Year: 2012
    ..g., x-ray crystallography, nuclear magnetic resonance, cryoelectron microscopy) will be derived from study of the gas phase protein complexes...
  41. Actin Dynamics, Interactions and Function
    Emil Reisler; Fiscal Year: 2013
    ..The emphasis will be on the changes in the interprotomer contact regions in actin filaments and on mapping the interaction interface on both proteins by cross-linking and mass spectrometry methods. ..
  42. High-Resolution CryoEM Reconstruction of Large Complexes
    Z Hong Zhou; Fiscal Year: 2013
    ..Moreover, the ability to look at large complexes at atomic resolution will permit visualization of complexes bound to antibodies, receptors, and drugs. ..
  43. Biochemical and structural characterization of self-assembling viral particles
    REBECCA ETHEL TAUROG; Fiscal Year: 2010
    ..assembly and determine the structure of both the coat protein by X-ray crystallography and the VLP by cryoelectron microscopy (cryo-EM)...
  44. Single-particle Reconstruction of HIV-1 Envelope Glycoprotein Trimers
    Joseph G Sodroski; Fiscal Year: 2013
    ..The proposed studies will utilize single-particle cryoelectron microscopy to yield structures of membrane-anchored HIV-1 Env trimers in different conformations at near-atomic (3...
  45. Towards the Disruption of Viral RNA Replication
    Esther Bullitt; Fiscal Year: 2013
    ....
  46. AAV capsids and their cellular interactions
    Mavis Agbandje-McKenna; Fiscal Year: 2013
    ....
  47. Molecular Mechanism of Translocon Assembly into Cell Plasma Membranes
    ALEJANDRO PABLO HEUCK; Fiscal Year: 2013
    ....
  48. Structure and Function of Flaviviruses
    Michael G Rossmann; Fiscal Year: 2013
    ..This should initiate fusion with the host cell endoplasmi membrane during endocytosis and, eventually, trigger release of the viral genome into the host cell's cytoplasm. ..
  49. Transcontinental EM Initiative for Membrane Protein Structure
    David L Stokes; Fiscal Year: 2013
    ..abstract_text> ..
  50. The Structural Basis of Protein Biogenesis
    Christopher W Akey; Fiscal Year: 2012
    ..An understanding of these transport channels will provide a framework for studying cellular defects in the relevant pathways. For example, mis-transport of human PrP (prion protein) has been implicated in neurodegenerative disease. ..
  51. DNA confined by surfaces
    Bernard Montgomery Pettitt; Fiscal Year: 2013
    ..Ultimately, we will test hypotheses related to the amount of disorder, ion screening and the contribution of DNA-protein confinement interactions. ..
  52. Structure and Function of the Herpesvirus Capsid and its DNA-Packaging Machinery
    James F Conway; Fiscal Year: 2013
    ..This proposal will, for example, inform efforts to interfere with assembly, such as by revealing subunit interfaces that may be targeted to inhibit binding. ..
  53. EUKARYOTIC PHOSPHOFRUCTOKINASE: STRUCTURE/FUNCTION
    Teresa Ruiz; Fiscal Year: 2009
    ..These studies will provide significant new information regarding the structure/function relationship of the mechanism of catalysis and regulation of phosphofructokinase in eukaryotic organisms. ..
  54. PREDOCTORAL TRAINING IN BIOLOGY AND CHEMISTRY
    PAUL WARREN STERNBERG; Fiscal Year: 2013
    ..Multi-user facilities include cell sorting, biological imaging including cryoelectron microscopy, NMR and mass spectrometry, monoclonal antibody production, high throughput DMA sequencing, animal care ..
  55. Structural studies of Togaviruses
    Michael G Rossmann; Fiscal Year: 2013
    ..We are planning to determine the remaining unknown structures of the Rubella virus components and use both crystallography and electron microscopy to study their interactions with each other and with antibodies. ..
  56. HIVRAD: Structural approaches to vaccine development
    Ellis Reinherz; Fiscal Year: 2009
    ..of investigators will utilize their collective talents in structural immunology, crystallography, NMR, cryoelectron microscopy and vaccinology to pursue structure-based subunit vaccine design...
  57. Molecular Biophysics Training Grant at Vanderbilt
    Walter J Chazin; Fiscal Year: 2013
    ..Such knowledge provides a direct understanding of normal physiology and the malfunctions that lead to disease, and in turn can be used to develop drug therapies targeted specifically for the defective molecules...
  58. Mechanistic Analysis Of Microtubule Based Motors
    Susan P Gilbert; Fiscal Year: 2013
    ..This comprehensive analysis of Kinesin-14 and Kinesin-2 members will provide new insights to understand the mechanochemistry that underlies structure-function relationships required for cellular organization and function. ..
  59. Acquisition of a Cryo-Electron Microscope
    Susan Hafenstein; Fiscal Year: 2012
    ..We identify 9 labs and describe 12 projects ready for data collection and data processing using cryoEM single particle reconstruction and tomography techniques. ..
  60. Towards a structural understanding of childhood obesity in Bardet-Biedl syndrome
    Susan E White; Fiscal Year: 2011
    ..In this project, we will examine how a stable particle of BBS proteins transports body weight sensors to the cellular antenna. Understanding how body weight is regulated may lead to new therapies for obesity. ..
  61. Structural Studies of Eukaryotic Transcription
    Francisco J Asturias; Fiscal Year: 2012
    ..Completion of these aims will provide structural information critical for unraveling the mechanism of transcription regulation. ..
  62. Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery
    Barton F Haynes; Fiscal Year: 2013
    ..define structures of protective HIV-1 envelope (Env) epitopes using crystallography and single particle cryoelectron microscopy, design vaccine strategies for driving antibody maturation pathways of difficult-to-induce neutralizing ..
  63. A comparative structural study of ATP-dependent chromatin remodeling complexes
    Andres Leschziner; Fiscal Year: 2013
    ..We aim to understand, at the molecular level, how these complexes interact with and remodel nucleosomes. Our research could lead to new insights in the fields of epigenetics and cancer. ..
  64. Three-Dimensional Imaging of Whole Cells by Using X-ray Diffraction Microscopy
    Jianwei Miao; Fiscal Year: 2010
    ..This novel imaging technique will be applied to the localization of multiprotein complexes inside whole cells. ..
  65. CORONAVIRUS-RECEPTOR INTERACTIONS
    Kathryn Holmes; Fiscal Year: 2003
    ..Inhibition of HCoV-229E respiratory infection by candidate drugs will be studied in transgenic mice that express the HCoV-229E receptor, hAPN, in the respiratory epithelium. ..
  66. STRUCTURAL BASIS OF KINESIN MOTOR MOVEMENT
    Hernando Sosa; Fiscal Year: 2006
    ..abstract_text> ..
  67. ALIMENTARY TRACT LIPIDS IN HEALTH AND DISEASE
    Martin Carey; Fiscal Year: 2003
    ..They will use I)novel flurocholesterol methodology, cryoelectron microscopy and electron energy-loss spectroscopy to elucidate physical-chemical pathways whereby cholesterol ..
  68. Protein interactions underlying actin-based motility
    David Sept; Fiscal Year: 2006
    ..This work will give perspective, not only into dendritic nucleation, but the motility of bacteria such as Listeria, and the function of the cell as a whole. ..
  69. 3D ELECTRON MICROSCOPY OF PYRUVATE DEHYDROGENASE
    JAMES STOOPS; Fiscal Year: 2001
    ..A 3-D reconstruction of the beef kidney intact PDC will permit a comparison of the mammalian and yeast complexes. ..
  70. Structual Analysis of Enterovirus Cell Entry Pathways
    JAMES HOGLE; Fiscal Year: 2002
    ..and cell entry intermediates will be extended to the highest possible resolution using a combination of cryoelectron microscopy and x-ray crystallography...
  71. INTERMEDIATES AND TRANSBILAYER PEPTIDES
    Martin Caffrey; Fiscal Year: 2001
    ..Recent time-resolved cryoelectron microscopy results show that transient, ordered arrays of fusion intermediates exist under special circumstances in ..
  72. DNA TOPOISOMERASES--BIOLOGICAL FUNCTIONS AND MECHANISMS
    James Wang; Fiscal Year: 1999
    ..2) determine the three-dimensional structure of the motor complex to 20 Angstroms or better, using cryoelectron microscopy; (3) attempt to correlate structure and function by analyzing images of the motor complex from mutant ..
  73. STRUCTURE-FUNCTION ANALYSIS OF ENVELOPED VIRUSES
    Wah Chiu; Fiscal Year: 1991
    ..These data will enhance our understanding of the packaging of the viral genome and the potential role of N protein in the regulation of transcription and replication...
  74. Cellular Footprinting of Transferrin-Receptor Complex
    MARK CHANCE; Fiscal Year: 2005
    ..abstract_text> ..