electron microscopy

Summary

Summary: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.

Top Publications

  1. ncbi XMIPP: a new generation of an open-source image processing package for electron microscopy
    C O S Sorzano
    Unidad de Biocomputación, Centro Nacional de Biotecnologia CSIC, Campus Universidad Autónoma s n, 28049 Cantoblanco, Madrid, Spain
    J Struct Biol 148:194-204. 2004
  2. ncbi FtsZ ring structure associated with division in Escherichia coli
    E F Bi
    Department of Microbiology, University of Kansas Medical Center, Kansas City 66103
    Nature 354:161-4. 1991
  3. ncbi Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation
    Jose M Mancheno
    Grupo de Cristalografia Macromolecular y Biologia Estructural, Instituto Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain
    Structure 11:1319-28. 2003
  4. pmc Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation
    T M Svitkina
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Cell Biol 139:397-415. 1997
  5. ncbi Automated electron microscope tomography using robust prediction of specimen movements
    David N Mastronarde
    Boulder Laboratory for Three Dimensional Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Struct Biol 152:36-51. 2005
  6. ncbi Curvature of clathrin-coated pits driven by epsin
    Marijn G J Ford
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 419:361-6. 2002
  7. ncbi Accurate determination of local defocus and specimen tilt in electron microscopy
    Joseph A Mindell
    Membrane Transport Biophysics Unit, National Institute of Neurological Disease and Stroke, National Institutes of Health, 36 Convent Drive, MSC4066, Bethesda, MD 20892 4066, USA
    J Struct Biol 142:334-47. 2003
  8. pmc Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes
    Nadine Herold
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Mol Cell Biol 29:281-301. 2009
  9. doi Atom-by-atom structural and chemical analysis by annular dark-field electron microscopy
    Ondrej L Krivanek
    Nion Co, 1102 8th Street, Kirkland, Washington 98033, USA
    Nature 464:571-4. 2010
  10. pmc RNA replication of mouse hepatitis virus takes place at double-membrane vesicles
    Rainer Gosert
    Institute for Medical Microbiology, University of Basel, Basel, Switzerland
    J Virol 76:3697-708. 2002

Research Grants

  1. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
  2. Role of Txnip in adapting fuel metabolism to nutritional state
    SIMON HUI; Fiscal Year: 2013
  3. Inhibition of human neutrophil function by Francisella tularensis
    Lee Ann H Allen; Fiscal Year: 2012
  4. Steroid Responsive Mechanisms in the Ear
    DENNIS ROYAL TRUNE; Fiscal Year: 2012
  5. ARNULF HANS WERNER KOEPPEN; Fiscal Year: 2014
  6. Present Homologous and Heterologous Antigen with Hepatitis E Virus
    R Holland Cheng; Fiscal Year: 2012
  7. CHARLES RICHARD STEELE; Fiscal Year: 2016
  8. Phillip Darwin Bell; Fiscal Year: 2016
  9. MELINDA ANN FRYE; Fiscal Year: 2014
  10. Pilot Studies of the Effect of Aging on Mucociliary Clearance
    Lawrence E Ostrowski; Fiscal Year: 2013

Detail Information

Publications423 found, 100 shown here

  1. ncbi XMIPP: a new generation of an open-source image processing package for electron microscopy
    C O S Sorzano
    Unidad de Biocomputación, Centro Nacional de Biotecnologia CSIC, Campus Universidad Autónoma s n, 28049 Cantoblanco, Madrid, Spain
    J Struct Biol 148:194-204. 2004
    ..the 3D reconstruction of biological specimens from large sets of projection images acquired by transmission electron microscopy. This public-domain software package was introduced to the electron microscopy field eight years ago, and ..
  2. ncbi FtsZ ring structure associated with division in Escherichia coli
    E F Bi
    Department of Microbiology, University of Kansas Medical Center, Kansas City 66103
    Nature 354:161-4. 1991
    ..The results suggest that FtsZ self-assembles into a ring structure at the future division site and may function as a cytoskeletal element. The formation of this ring may be the point at which division is regulated...
  3. ncbi Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation
    Jose M Mancheno
    Grupo de Cristalografia Macromolecular y Biologia Estructural, Instituto Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain
    Structure 11:1319-28. 2003
    ..7 A and 18 A resolution, respectively, by using X-ray crystallography and electron microscopy of two-dimensional crystals...
  4. pmc Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation
    T M Svitkina
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Cell Biol 139:397-415. 1997
    ..We propose that the forward translocation of the cell body and retrograde flow in the lamellipodia are both driven by contraction of an actin-myosin network in the lamellipodial/cell body transition zone...
  5. ncbi Automated electron microscope tomography using robust prediction of specimen movements
    David N Mastronarde
    Boulder Laboratory for Three Dimensional Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Struct Biol 152:36-51. 2005
    ..It also includes tools for navigating between specimen positions and finding regions of interest...
  6. ncbi Curvature of clathrin-coated pits driven by epsin
    Marijn G J Ford
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 419:361-6. 2002
    ..We propose that this helix is inserted into one leaflet of the lipid bilayer, inducing curvature. On lipid monolayers epsin alone is sufficient to facilitate the formation of clathrin-coated invaginations...
  7. ncbi Accurate determination of local defocus and specimen tilt in electron microscopy
    Joseph A Mindell
    Membrane Transport Biophysics Unit, National Institute of Neurological Disease and Stroke, National Institutes of Health, 36 Convent Drive, MSC4066, Bethesda, MD 20892 4066, USA
    J Struct Biol 142:334-47. 2003
    ..is essential for the determination of three-dimensional protein structures at high resolution using electron microscopy. We present two computer programs, CTFFIND3 and CTFTILT, which determine defocus parameters from images of ..
  8. pmc Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes
    Nadine Herold
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Mol Cell Biol 29:281-301. 2009
    ..b>Electron microscopy of Drosophila B complexes revealed a high degree of structural similarity with human B complexes, ..
  9. doi Atom-by-atom structural and chemical analysis by annular dark-field electron microscopy
    Ondrej L Krivanek
    Nion Co, 1102 8th Street, Kirkland, Washington 98033, USA
    Nature 464:571-4. 2010
    ..Transmission electron microscopy should in principle be able to fulfil this role, as many scientists including Feynman realized early on...
  10. pmc RNA replication of mouse hepatitis virus takes place at double-membrane vesicles
    Rainer Gosert
    Institute for Medical Microbiology, University of Basel, Basel, Switzerland
    J Virol 76:3697-708. 2002
    ..whether MHV proteins and viral RNA were associated with the DMVs, we performed immunocytochemistry electron microscopy (IEM)...
  11. pmc Electron microscopy of whole cells in liquid with nanometer resolution
    N de Jonge
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232 0615, USA
    Proc Natl Acad Sci U S A 106:2159-64. 2009
    ..The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods...
  12. pmc Three-dimensional structure of A1A0 ATP synthase from the hyperthermophilic archaeon Pyrococcus furiosus by electron microscopy
    Janet Vonck
    Max Planck Institute of Biophysics, Max von Laue Strasse 3, D 60438 Frankfurt am Main, Germany
    J Biol Chem 284:10110-9. 2009
    ..A three-dimensional map was generated by electron microscopy from negatively stained images. The map at a resolution of 2...
  13. pmc Refolding of a paramyxovirus F protein from prefusion to postfusion conformations observed by liposome binding and electron microscopy
    Sarah A Connolly
    Howard Hughes Medical Institute, Northwestern University, Evanston, IL 60208 3500, USA
    Proc Natl Acad Sci U S A 103:17903-8. 2006
    ..b>Electron microscopy data showed that triggered F formed rosettes...
  14. pmc Three-dimensional electron microscopy reveals new details of membrane systems for Ca2+ signaling in the heart
    Takeharu Hayashi
    The Center for Research in Biological Systems, University of California San Diego, La Jolla, CA 92093, USA
    J Cell Sci 122:1005-13. 2009
    ..g. Ca(2+) sparks)...
  15. ncbi Occludin as a possible determinant of tight junction permeability in endothelial cells
    T Hirase
    Eisai London Research Laboratories Ltd, University College London, UK
    J Cell Sci 110:1603-13. 1997
    ..Our data indicate that regulation of occludin expression may be a crucial determinant of the tight junction permeability properties of endothelial cells in different tissues...
  16. pmc The ATP synthase is involved in generating mitochondrial cristae morphology
    Patrick Paumard
    Institut de Biochimie et Genetique Cellulaires du CNRS, Universite Victor Segalen, Bordeaux 2, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
    EMBO J 21:221-30. 2002
    ....
  17. ncbi A comparison of muscle thin filament models obtained from electron microscopy reconstructions and low-angle X-ray fibre diagrams from non-overlap muscle
    Katrina J V Poole
    Arbeitsgruppe Biophysik, Max Planck Institut fur medizinische Forschung, 69120 Heidelberg, Germany
    J Struct Biol 155:273-84. 2006
    ....
  18. ncbi Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway
    J H Rothman
    Cell 47:1041-51. 1986
    ..Thus vpl mutations define a new class of genes that encode products required for sorting of newly synthesized vacuolar proteins from secretory proteins during their transit through the yeast secretory pathway...
  19. pmc Isolation of the yeast nuclear pore complex
    M P Rout
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021
    J Cell Biol 123:771-83. 1993
    Nuclear pore complexes (NPCs) have been isolated from the yeast Saccharomyces. Negative stain electron microscopy of the isolated NPCs and subsequent image reconstruction revealed the octagonal symmetry and many of the ultrastructural ..
  20. ncbi Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy
    P I Hanson
    Department of Pharmacology and HHMI, Yale University School of Medicine, New Haven, CT 06510, USA
    Cell 90:523-35. 1997
    Using quick-freeze/deep-etch electron microscopy of recombinant proteins adsorbed to mica, we show that NSF, the oligomeric ATPase involved in membrane fusion, is a hollow 10 x 16 nm cylinder whose conformation depends upon nucleotide ..
  21. ncbi Three-dimensional reconstruction of glomeruli by electron microscopy reveals a distinct restrictive urinary subpodocyte space
    Christopher R Neal
    Microvascular Research Laboratories, Department of Physiology, Preclinical Veterinary School, University of Bristol, Southwell Street, Bristol BS2 8EJ, UK
    J Am Soc Nephrol 16:1223-35. 2005
    ..The physiologic role of the restrictive SPS is yet to be determined but likely possibilities include regulation of glomerular filtration and cleaning of the glomerular filtration barrier...
  22. ncbi Bsoft: image processing and molecular modeling for electron microscopy
    J Bernard Heymann
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 157:3-18. 2007
    ..B., 2001. Bsoft: image and molecular processing in electron microscopy. J. Struct. Biol. 133, 156-169]...
  23. pmc Image processing for electron microscopy single-particle analysis using XMIPP
    Sjors H W Scheres
    Centro Nacional de Biotecnologia CSIC, Unidad de Biocomputación, Cantoblanco, 28049 Madrid, Spain
    Nat Protoc 3:977-90. 2008
    We describe a collection of standardized image processing protocols for electron microscopy single-particle analysis using the XMIPP software package...
  24. doi Confocal/TEM overlay microscopy: a simple method for correlating confocal and electron microscopy of cells expressing GFP/YFP fusion proteins
    Douglas R Keene
    Research Department, Shriners Hospital for Children, Portland, OR 97239, USA
    Microsc Microanal 14:342-8. 2008
    ..and cell cultures has become routine, but correlation of light microscopy and high resolution transmission electron microscopy (TEM) on components within identical cells has been problematic...
  25. doi Discovery of a new RNA-containing nuclear structure in UVC-induced apoptotic cells by integrated laser electron microscopy
    Matthia A Karreman
    Electron Microscopy and Structure Analysis, Cellular Architecture and Dynamics, Utrecht University, Padualaan 8, NL 3584 CH Utrecht, The Netherlands
    Biol Cell 101:287-99. 2009
    ..morphological markers of apoptosis using a new method of correlative microscopy, ILEM (integrated laser electron microscopy). Cleaved caspase 3 and gamma-H2AX were immunofluorescently labelled to mark the cells of interest...
  26. pmc Temporal analysis of the developing Chlamydia psittaci inclusion by use of fluorescence and electron microscopy
    D D Rockey
    Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA
    Infect Immun 64:4269-78. 1996
    ..The division of inclusions slows or stops in mid-cycle, and dividing RB accumulate within the enlarging lobes. The RB then differentiate to EBs, the inclusion and cell are lysed, and EBs are freed to infect another cell...
  27. ncbi Mitochondrial energetics in the heart in obesity-related diabetes: direct evidence for increased uncoupled respiration and activation of uncoupling proteins
    Sihem Boudina
    Division of Endocrinology, Metabolism, and Diabetes, Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Diabetes 56:2457-66. 2007
    ..Mitochondrial uncoupling has been proposed to contribute to these metabolic abnormalities but has not been directly demonstrated...
  28. doi The role played by electron microscopy in advancing our understanding of Toxoplasma gondii and other apicomplexans
    Jean Francois Dubremetz
    UMR CNRS, Universite de Montpellier, France
    Int J Parasitol 39:883-93. 2009
    ..ways the history of the discovery of the life cycle of Toxoplasma gondii and the development of biological electron microscopy progressed in parallel through the 1950s and 1960s...
  29. pmc Tissue-specific remodeling of the mitochondrial proteome in type 1 diabetic akita mice
    Heiko Bugger
    Division of Endocrinology, Metabolism and Diabetes, and Program in Molecular Medicine, University of Utah School of Medicine, Salt Lake City, Utah, USA
    Diabetes 58:1986-97. 2009
    ..To elucidate the molecular basis for mitochondrial dysfunction, which has been implicated in the pathogenesis of diabetes complications...
  30. doi Electron microscopy of Xrcc4 and the DNA ligase IV-Xrcc4 DNA repair complex
    María A Recuero-Checa
    Centro de Investigaciones Biologicas CIB, Consejo Superior de Investigaciones Cientificas CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain
    DNA Repair (Amst) 8:1380-9. 2009
    ..have purified full length Xrcc4 and the Lig4-Xrcc4 complex, and analysed their structure by single-particle electron microscopy. The three-dimensional structure of Xrcc4 at a resolution of approximately 37A reveals that the C-terminus ..
  31. pmc Electron microscopy and persistence length analysis of semi-rigid smooth muscle tropomyosin strands
    Duncan Sousa
    Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts, USA
    Biophys J 99:862-8. 2010
    ..40 nm long smooth and striated muscle tropomyosin molecules were rotary-shadowed and compared by means of electron microscopy. Electron microscopy shows that striated muscle tropomyosin primarily consists of single molecules or ..
  32. pmc Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau
    D N Drechsel
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143
    Mol Biol Cell 3:1141-54. 1992
    ..Thus, tau generates a partially stable but still dynamic state in microtubules. This state is perturbed by phosphorylation by MAP2 kinase, which affects all three activities by lowering the affinity of tau for the microtubule lattice...
  33. pmc Onset of re-epithelialization after skin injury correlates with a reorganization of keratin filaments in wound edge keratinocytes: defining a potential role for keratin 16
    R D Paladini
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 132:381-97. 1996
    ....
  34. pmc Regulation of membrane trafficking and subcellular organization of endocytic compartments revealed with FM1-43 in resting and activated human T cells
    Alla F Fomina
    Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
    Exp Cell Res 291:150-66. 2003
    ..The present study demonstrates the utility of FM1-43 as a marker of membrane trafficking in T cells and reveals possible mechanisms of its modulation during T cell activation...
  35. ncbi MAP2c, but not tau, binds and bundles F-actin via its microtubule binding domain
    Benoit Roger
    Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Biol 14:363-71. 2004
    ..MAPs represent good candidates to mediate such interactions. Although MAP2c and tau have similar, well-characterized microtubule binding activities, their actin interaction is poorly understood...
  36. ncbi Three-dimensional model of purple membrane obtained by electron microscopy
    R Henderson
    Nature 257:28-32. 1975
    A 7-A resolution map of the purple membrane has been obtained by electron microscopy of tilted, unstained specimens...
  37. ncbi Small GTP-binding protein associated with Golgi cisternae
    B Goud
    Unité de Génétique Somatique URA CNRS 361, Institut Pasteur, Paris, France
    Nature 345:553-6. 1990
    ..By immunolabelling and electron microscopy, Rab6p appears to be concentrated predominantly on the medial and trans cisternae and distributed over ..
  38. pmc Differences in the endosomal distributions of the two mannose 6-phosphate receptors
    J Klumperman
    Department of Cell Biology, Utrecht University, The Netherlands
    J Cell Biol 121:997-1010. 1993
    ..These data suggest that the two MPRs exit the TGR via the same coated vesicles, but that upon arrival in the endosomes CD-MPR is more rapidly than CI-MPR, segregated into ATV which probably are destined to recycle MPRs to TGR...
  39. ncbi ARPE-19, a human retinal pigment epithelial cell line with differentiated properties
    K C Dunn
    Department of Ophthalmology, University of California, Davis 95616, USA
    Exp Eye Res 62:155-69. 1996
    ..Our results demonstrate that ARPE-19 has structural and functional properties characteristic of RPE cells in vivo and suggest that this cell line will be valuable for in vitro studies of retinal pigment epithelium physiology...
  40. ncbi Role of Drosophila alpha-adaptin in presynaptic vesicle recycling
    M Gonzalez-Gaitan
    Max Planck Institut fur biophysikalische Chemie, Abt Molekulare Entwicklungsbiologie, Gottingen, Germany
    Cell 88:767-76. 1997
    ..Our results suggest an alpha-adaptin-dependent control of the vesicle cycle that maintains the balance between the amount of vesicle- and surface-associated membranes...
  41. ncbi Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cells
    T Self
    MRC Institute of Hearing Research, University Park, Nottingham NG7 2RD, UK
    Development 125:557-66. 1998
    ..These findings suggest that myosin VIIA is required for normal stereocilia bundle organisation and has a role in the function of cochlear hair cells...
  42. ncbi WNT signaling in the control of hair growth and structure
    S E Millar
    Howard Hughes Medical Institute, Stanford University, Stanford, California, 94305 5428, USA
    Dev Biol 207:133-49. 1999
    ....
  43. ncbi Occludin and claudins in tight-junction strands: leading or supporting players?
    S Tsukita
    Dept of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    Trends Cell Biol 9:268-73. 1999
    ..attracted much interest from cell biologists, especially electron microscopists, since on freeze-fracture electron microscopy they appear as a well-developed network of continuous, anastomosing intramembranous strands (tight-junction ..
  44. pmc PV-1 is a component of the fenestral and stomatal diaphragms in fenestrated endothelia
    R V Stan
    University of California at San Diego, Division of Cellular and Molecular Medicine, La Jolla, CA 92093 0651, USA
    Proc Natl Acad Sci U S A 96:13203-7. 1999
    ..They also show that the stomatal diaphragms of caveolae and transendothelial channels and the fenestral diaphragms might be biochemically related, in addition to being morphologically similar structures...
  45. ncbi E. coli hemolysin E (HlyE, ClyA, SheA): X-ray crystal structure of the toxin and observation of membrane pores by electron microscopy
    A J Wallace
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, United Kingdom
    Cell 100:265-76. 2000
    ..and the visualization of the lipid-associated form of the toxin in projection at low resolution by electron microscopy. The crystal structure reveals HlyE to be the first member of a new family of toxin structures, consisting ..
  46. pmc Extragenic bypass suppressors of mutations in the essential gene BLD2 promote assembly of basal bodies with abnormal microtubules in Chlamydomonas reinhardtii
    A M Preble
    Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
    Genetics 157:163-81. 2001
    ..It is likely that BLD2 is an essential gene that is needed for basal body assembly and function...
  47. ncbi Electron microscopy of oligodendroglia in severe mental illness
    N Uranova
    Laboratory of Neuropathology, Mental Health Research Center, Moscow, Russia
    Brain Res Bull 55:597-610. 2001
    Qualitative electron microscopy was performed to verify whether brain pathology in schizophrenia and bipolar disorder is associated with alterations of oligodendroglial cells and myelinated fibers...
  48. ncbi Uterine glands provide histiotrophic nutrition for the human fetus during the first trimester of pregnancy
    Graham J Burton
    Department of Anatomy, University of Cambridge, Downing Street, Cambridge CB2 3DY, United Kingdom
    J Clin Endocrinol Metab 87:2954-9. 2002
    ..Our findings demonstrate that the uterine glands are an important source of nutrients during organogenesis, when metabolism is essentially anaerobic...
  49. ncbi Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes
    Thomas Nebl
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Biol Chem 277:43399-409. 2002
    ..We suggest that the DRM-H fraction represents a membrane skeleton-associated subset of leukocyte signaling domains...
  50. ncbi Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation
    Richard Wubbolts
    Department of Cell Biology, Utrecht University Medical Centre and Institute of Biomembranes, Room G02 525, Heidelberglaan 100, 3585 CX Utrecht, The Netherlands
    J Biol Chem 278:10963-72. 2003
    ....
  51. ncbi Comparison of comet assay, electron microscopy, and flow cytometry for detection of apoptosis
    Shingo Yasuhara
    Department of Anesthesiology and Critical Care, Massachusetts General Hospital, Shriners Hospital for Children, and Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA
    J Histochem Cytochem 51:873-85. 2003
    ..evaluated the specificity of the neutral comet assay and flow cytometry compared to the gold standard, electron microscopy, for detection of apoptosis and necrosis...
  52. ncbi Human meiotic recombinase Dmc1 promotes ATP-dependent homologous DNA strand exchange
    Michael G Sehorn
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520, USA
    Nature 429:433-7. 2004
    ..The DNA strand exchange activity of hDmc1 is probably indispensable for repair of DNA double-strand breaks during meiosis and for maintaining the ploidy of meiotic chromosomes...
  53. ncbi Endocytic delivery to lysosomes mediated by concurrent fusion and kissing events in living cells
    Nicholas A Bright
    Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2XY, United Kingdom
    Curr Biol 15:360-5. 2005
    ..fusions were observed between endosomes and lysosomes, and one such event was captured by correlative electron microscopy. Fluorescence intensity analyses of endosomes that encountered lysosomes revealed a gradual accumulation of ..
  54. ncbi Correlative microscopy and electron tomography of GFP through photooxidation
    Markus Grabenbauer
    Cell Biology and Cell Biophysics Program, EMBL, Meyerhofstr 1, D 69117 Heidelberg, Germany
    Nat Methods 2:857-62. 2005
    ..3,3'-diaminobenzidine (DAB) into an electron-dense precipitate that can be visualized by routine electron microscopy and electron tomography...
  55. ncbi Role of myosin VI in the differentiation of cochlear hair cells
    T Self
    MRC Institute of Hearing Research, University Park, Nottingham, NG7 2RD, United Kingdom
    Dev Biol 214:331-41. 1999
    ....
  56. pmc Isoforms of alpha-actinin from cardiac, smooth, and skeletal muscle form polar arrays of actin filaments
    K A Taylor
    Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306 3015, USA
    J Cell Biol 149:635-46. 2000
    ..Formation of specifically bipolar or polar filament arrays cross-linked by alpha-actinin may require additional factors that either determine the filament orientation or restrict the cross-linking capabilities of alpha-actinin...
  57. ncbi Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4
    Joanna Timmins
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    J Mol Biol 326:493-502. 2003
    ..Single particle electron microscopy reconstructions indicate that WW3 of Nedd4 is in close contact with the N-terminal domain of hexameric VP40...
  58. pmc Infectious HIV-1 assembles in late endosomes in primary macrophages
    Annegret Pelchen-Matthews
    Cell Biology Unit, Medical Research MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK
    J Cell Biol 162:443-55. 2003
    ..Here, we investigated virus assembly in HIV-1-infected primary human monocyte-derived macrophages (MDM). Electron microscopy of cryosections showed virus particles, identified by their morphology and positive labeling with ..
  59. pmc LOW PSII ACCUMULATION1 is involved in efficient assembly of photosystem II in Arabidopsis thaliana
    Lianwei Peng
    Photosynthesis Research Center, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    Plant Cell 18:955-69. 2006
    ..Thus, LPA1 appears to be an integral membrane chaperone that is required for efficient PSII assembly, probably through direct interaction with the PSII reaction center protein D1...
  60. pmc Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy
    S Eskandari
    Department of Physiology, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095 1751, USA
    Proc Natl Acad Sci U S A 95:11235-40. 1998
    We have used freeze-fracture electron microscopy to examine the oligomeric structure and molecular asymmetry of integral plasma membrane proteins...
  61. ncbi The usefulness of bronchoalveolar lavage in identifying past occupational exposure to asbestos: a light and electron microscopy study
    R F Dodson
    Department of Cell Biology and Environmental Sciences, University of Texas Health Center, Tyler 75710
    Am J Ind Med 19:619-28. 1991
    ..The parameters used for distinguishing the source of these samples included both light microscopy assessment of the filters for the presence of ferruginous bodies and electron microscopic screening for the presence of uncoated fibers...
  62. pmc Rab7: a key to lysosome biogenesis
    C Bucci
    Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen, Denmark
    Mol Biol Cell 11:467-80. 2000
    ..These data demonstrate for the first time that Rab7, controlling aggregation and fusion of late endocytic structures/lysosomes, is essential for maintenance of the perinuclear lysosome compartment...
  63. ncbi Nipah virus: a recently emergent deadly paramyxovirus
    K B Chua
    Department of Medical Microbiology, University of Malaya Medical Center, 50603 Kuala Lumpur, Malaysia
    Science 288:1432-5. 2000
    ..Like Hendra virus, Nipah virus is unusual among the paramyxoviruses in its ability to infect and cause potentially fatal disease in a number of host species, including humans...
  64. ncbi A distinct pathway remodels mitochondrial cristae and mobilizes cytochrome c during apoptosis
    Luca Scorrano
    Howard Hughes Medical Institute, Department of Pathology and Medicine, Harvard Medical School, Dana Farber Cancer Institute, 02115, Boston, MA, USA
    Dev Cell 2:55-67. 2002
    ..This reorganization does not require tBID's BH3 domain and is independent of BAK, but is inhibited by CsA. During this process, individual cristae become fused and the junctions between the cristae and the intermembrane space are opened...
  65. pmc Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice
    Mikio Furuse
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 156:1099-111. 2002
    ..These findings provide the first evidence that continuous claudin-based TJs occur in the epidermis and that these TJs are crucial for the barrier function of the mammalian skin...
  66. ncbi ATP-mediated conformational changes in the RecA filament
    Margaret S VanLoock
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences, Charlottesville, VA 22908, USA
    Structure 11:187-96. 2003
    ..Using electron microscopy, we have reconstructed five different states of RecA-DNA filaments...
  67. pmc Full-length archaeal Rad51 structure and mutants: mechanisms for RAD51 assembly and control by BRCA2
    David S Shin
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 22:4566-76. 2003
    ..proteins assemble into inactive heptameric rings and active DNA-bound filaments matching three-dimensional electron microscopy reconstructions. A polymerization motif (RAD51-PM) tethers individual subunits together to form assemblies...
  68. ncbi AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding
    Bettina Strack
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 114:689-99. 2003
    ..These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III...
  69. pmc Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of São Paulo, Brazil, where Brazilian spotted fever is endemic
    Marcelo B Labruna
    Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555 0609, USA
    J Clin Microbiol 42:90-8. 2004
    ..These results do not support the role of A. cooperi in the ecology of R. rickettsii in the area studied, but they add two more species of rickettsiae to the poorly developed list of species occurring in ticks in South America...
  70. pmc Neuregulin-1 type III determines the ensheathment fate of axons
    Carla Taveggia
    Department of Cell Biology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
    Neuron 47:681-94. 2005
    ..These results indicate that levels of NRG1 type III, independent of axon diameter, provide a key instructive signal that determines the ensheathment fate of axons...
  71. ncbi A brief update on lung stereology
    Matthias Ochs
    Institute of Anatomy, Experimental Morphology Unit, University of Bern, Switzerland
    J Microsc 222:188-200. 2006
    ....
  72. pmc Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy
    Guido M Gaietta
    National Center for Microscopy and Imaging Research, Center for Research on Biological Structure, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:17777-82. 2006
    ..of diaminobenzidine, allowing 4D optical recording on live cells and correlated ultrastructural analysis by electron microscopy. These methods reveal that Golgi reassembly is preceded by the formation of four colinear clusters at ..
  73. ncbi Gliotactin, a novel transmembrane protein on peripheral glia, is required to form the blood-nerve barrier in Drosophila
    V J Auld
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Cell 81:757-67. 1995
    ..As a result, peripheral motor axons are exposed to the high K+ hemolymph, action potentials fail to propagate, and the embryos are nearly paralyzed...
  74. pmc Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes
    V Laurent
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    J Cell Biol 144:1245-58. 1999
    ..We propose a molecular ratchet model for insertional polymerization of actin, within which frequent attachment-detachment of VASP to F-actin allows its sliding along the growing filament...
  75. pmc Observing FcepsilonRI signaling from the inside of the mast cell membrane
    B S Wilson
    Department of Pathology and Cancer Research and Treatment Center, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, 87131, USA
    J Cell Biol 149:1131-42. 2000
    ..The sequential association of FcstraightepsilonRI with Lyn, Syk, and coated pits in topographically distinct membrane domains implicates membrane segregation in the regulation of FcstraightepsilonRI signaling...
  76. ncbi A novel coronavirus associated with severe acute respiratory syndrome
    Thomas G Ksiazek
    Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 348:1953-66. 2003
    ..We conducted studies to identify the etiologic agent of this outbreak...
  77. pmc Herpes simplex virus gE/gI sorts nascent virions to epithelial cell junctions, promoting virus spread
    D C Johnson
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Virol 75:821-33. 2001
    ..Delivery of virus particles to cell junctions would be expected to enhance virus spread and enable viruses to avoid host immune defenses...
  78. ncbi New electron microscopy database and deposition system
    Mohamed Tagari
    European Bioinformatics Institute, EMBL Outstation Hinxton, Wellcome Trust Genome Campus, Cambridge, UK CB10 1SD
    Trends Biochem Sci 27:589. 2002
    To manage, organize and disseminate data on the structure of biological macromolecules solved by 3D electron microscopy, an electron microscopy database has been set up at the European Bioinformatics Institute.
  79. ncbi Macropinocytosis is the endocytic pathway that mediates macrophage foam cell formation with native low density lipoprotein
    Howard S Kruth
    Section of Experimental Atherosclerosis, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1422, USA
    J Biol Chem 280:2352-60. 2005
    ..Macropinocytosis in macrophages and possibly other vascular cells is a new pathway to target for modulating foam cell formation in atherosclerosis...
  80. ncbi Bcl-xL regulates the membrane potential and volume homeostasis of mitochondria
    M G Vander Heiden
    Gwen Knapp Center and Committee on Immunology, Department of Medicine, University of Chicago, Illinois 60637, USA
    Cell 91:627-37. 1997
    ..These data suggest that Bcl-xL promotes cell survival by regulating the electrical and osmotic homeostasis of mitochondria...
  81. ncbi The yeast phosphatidylinositol-4-OH kinase pik1 regulates secretion at the Golgi
    C Walch-Solimena
    Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06510, USA
    Nat Cell Biol 1:523-5. 1999
  82. ncbi Synapses on human spiral ganglion cells: a transmission electron microscopy and immunohistochemical study
    H Rask-Andersen
    Department of Otolaryngology, University Hospital Akademiska sjukhuset of Uppsala, 751 85, Uppsala, Sweden
    Hear Res 141:1-11. 2000
    A transmission electron microscopy (TEM) study and synaptophysin immunoreactivity analysis of neurons in the human spiral ganglion was performed with particular emphasis on the demonstration of synapses...
  83. ncbi Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid membrane of green plant chloroplasts
    E J Boekema
    Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, 9747 AG, The Netherlands
    J Mol Biol 301:1123-33. 2000
    ..the structural organization of Photosystem II (PSII) in paired grana membrane fragments by transmission electron microscopy. The membrane fragments were obtained by a short treatment of thylakoid membranes with the mild detergent n-..
  84. ncbi A recombinant particulate antigen of Japanese encephalitis virus produced in stably-transformed cells is an effective noninfectious antigen and subunit immunogen
    A R Hunt
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, PO Box 2087, Fort Collins, CO 80522 2087, USA
    J Virol Methods 97:133-49. 2001
    ..Extracellular particles purified by equilibrium density centrifugation in sucrose gradients followed by electron microscopy were characterized as spherical particles with an average diameter of approximately 30 nm and a buoyant ..
  85. ncbi Comparison of bacteriophage T4 UvsX and human Rad51 filaments suggests that RecA-like polymers may have evolved independently
    S Yang
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0733, USA
    J Mol Biol 312:999-1009. 2001
    ..Like RecA, Rad51 and RadA, UvsX forms helical filaments on DNA. We have used electron microscopy and a novel method for image analysis of helical filaments to show that UvsX-DNA filaments exist in two ..
  86. ncbi Basement membrane of mouse bone marrow sinusoids shows distinctive structure and proteoglycan composition: a high resolution ultrastructural study
    S Inoue
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 2B2
    Anat Rec 264:294-304. 2001
    ..specialization, the basement membrane of bone marrow sinusoids has now been examined by high resolution electron microscopy of perfusion-fixed mouse bone marrow...
  87. ncbi Tissue engineering of a differentiated cardiac muscle construct
    W H Zimmermann
    Institute of Experimental and Clinical Pharmacology and Toxicology, Friedrich Alexander University of Erlangen Nuremberg, Erlangen, Germany
    Circ Res 90:223-30. 2002
    ..Confocal and electron microscopy demonstrated characteristic features of native differentiated myocardium; some of these features are absent ..
  88. ncbi Using rational screening and electron microscopy to optimize the crystallization of succinate:ubiquinone oxidoreductase from Escherichia coli
    Rob Horsefield
    Department of Biological Sciences, Imperial College, London SW7 2AZ, England
    Acta Crystallogr D Biol Crystallogr 59:600-2. 2003
    ..In order to facilitate structure determination, rational screening and sample-quality analysis using electron microscopy was implemented. The crystals of SQR from E...
  89. pmc Two distinct size classes of immature and mature subviral particles from tick-borne encephalitis virus
    Steven L Allison
    Institute of Virology, University of Vienna, A 1095 Vienna, Austria
    J Virol 77:11357-66. 2003
    ..b>Electron microscopy showed that the smaller particles were approximately the same size as the previously described mature RSPs, ..
  90. ncbi Targeted inactivation of murine laminin gamma2-chain gene recapitulates human junctional epidermolysis bullosa
    Xianmin Meng
    Department of Dermatology and Cutaneous Biology, Jefferson Medical College, and Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Invest Dermatol 121:720-31. 2003
    ..Transmission electron microscopy revealed rudimentary, poorly developed hemidesmosomes...
  91. ncbi Phosphorylcholine-based pH-responsive diblock copolymer micelles as drug delivery vehicles: light scattering, electron microscopy, and fluorescence experiments
    Cristiano Giacomelli
    Laboratoire de Chimie des Polymeres Organiques, LCPO ENSCPB, Universite Bordeaux 1, 16 Av Pey Berland, 33607 Pessac Cedex, France
    Biomacromolecules 7:817-28. 2006
    ..block (PMPC-b-PDPA) has been studied by static and dynamic light scattering (SDLS), transmission electron microscopy (TEM), and potentiometry...
  92. ncbi Comparison of the prevalence of enteric viruses in healthy dogs and those with acute haemorrhagic diarrhoea by electron microscopy
    B S Schulz
    Clinic of Small Animal Medicine, Ludwig Maximilian University Munich, Veterinarstrasse 13, 80539, Germany
    J Small Anim Pract 49:84-8. 2008
    ..To evaluate prevalence of enteric viruses in healthy dogs and to compare it with prevalences in dogs with acute haemorrhagic diarrhoea...
  93. pmc Plasma membrane deformation by circular arrays of ESCRT-III protein filaments
    Phyllis I Hanson
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 180:389-402. 2008
    ..We used "deep-etch" electron microscopy to examine polymers formed by the ESCRT-III proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B)...
  94. doi Structural analysis of the Saf pilus by electron microscopy and image processing
    Osman Salih
    Institute of Structural Molecular Biology at UCL Birkbeck, London WC1E 7HX, UK
    J Mol Biol 379:174-87. 2008
    ..In our study, we have used negative stain electron microscopy and single-particle image analysis to determine the three-dimensional structure of the Salmonella ..
  95. doi Basic phage electron microscopy
    Hans W Ackermann
    Department of Medical Microbiology, Faculty of Medicine, Laval University, Quebec, QC, Canada
    Methods Mol Biol 501:113-26. 2009
    ..The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts...
  96. doi Electron and X-ray methods of ultrafast structural dynamics: advances and applications
    Majed Chergui
    Laboratoire de spectroscopie ultrarapide Ecole Polytechnique Fédérale de Lausanne, ISIC FSB BSP, CH 1015 Lausanne, Switzerland
    Chemphyschem 10:28-43. 2009
    ..Although this article is not a survey of all the work done with these techniques, it provides a comprehensive referencing to current research...
  97. pmc SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs
    Tanvir R Shaikh
    Wadsworth Center, Empire State Plaza, Albany, New York 12201 0509, USA
    Nat Protoc 3:1941-74. 2008
    ..Also described is supervised classification, a method to look for homogeneous subsets when multiple known conformations of the molecule may coexist...
  98. doi Qualitative and quantitative characterization of autophagy in Caenorhabditis elegans by electron microscopy
    Timea Sigmond
    Laboratory of Developmental Genetics, Department of Genetics, Eotvos Lorand University, Budapest, Hungary
    Methods Enzymol 451:467-91. 2008
    ..Therefore, it has to be studied in parallel with both traditional electron microscopy and modern molecular approaches...
  99. ncbi Electron microscopy in antiparasitic chemotherapy: a (close) view to a kill
    M A Vannier-Santos
    Laboratório de Biomorfologia Parasitária, Unidade de Microscopia Eletrônica, Centro de Pesquisa Gonçalo Moniz, Fundacao Oswaldo Cruz, Salvador, BA, Brazil
    Curr Drug Targets 10:246-60. 2009
    b>Electron microscopy may be useful in chemotherapy studies at distinct levels, such as the identification of subcellular targets in the parasites and the elucidation of the ultimate drug mechanism of action, inferred by the alterations ..
  100. doi Electron microscopy and cytochemistry analysis of the endocytic pathway of pathogenic protozoa
    Wanderley de Souza
    Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Cidade Universitaria, Ilha do Fundao, Rio de Janeiro 21941 902, Brazil
    Prog Histochem Cytochem 44:67-124. 2009
    ....
  101. pmc Enhanced imaging in low dose electron microscopy using electron counting
    G McMullan
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Ultramicroscopy 109:1411-6. 2009
    ..Using this approach we find enhancements in the Nyquist frequency modulation transfer function (MTF) and detective quantum efficiency (DQE) over the corresponding integrating mode values by factors of 8 and 3, respectively...

Research Grants73

  1. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..We will use both transmission and X- ray spectral electron microscopy to map the distributions of tagged lipids on membrane sheets...
  2. Role of Txnip in adapting fuel metabolism to nutritional state
    SIMON HUI; Fiscal Year: 2013
    ..b>Electron microscopy will be used to detect alterations in mitochondrial ultrastructure...
  3. Inhibition of human neutrophil function by Francisella tularensis
    Lee Ann H Allen; Fiscal Year: 2012
    ..species;confocal microscopy to localize NADPH oxidase components;immunofluorescence microscopy and immuno-electron microscopy to assess phagosome composition;transmission electron microscopy to quantify phagosome escape and PMN ..
  4. Steroid Responsive Mechanisms in the Ear
    DENNIS ROYAL TRUNE; Fiscal Year: 2012
    ..response audiometry and endocochlear potential;2) inner ear morphology will be assessed by light and electron microscopy;3) systemic autoimmune disease will be assessed by serum immune complexes, hematocrits, and antinuclear ..
  5. ARNULF HANS WERNER KOEPPEN; Fiscal Year: 2014
    ..microscopy to quantify nerve cell damage and loss of synaptic terminals in regions of increased iron;and electron microscopy to reveal cytosolic and mitochondrial ferritin...
  6. Present Homologous and Heterologous Antigen with Hepatitis E Virus
    R Holland Cheng; Fiscal Year: 2012
    ..The proposed experiments in this application include 1) using cryo-electron microscopy and image reconstruction to identify HEV-VLP surface flexible loops that critical to antibody interactions;..
  7. CHARLES RICHARD STEELE; Fiscal Year: 2016
    ..This will be tested using micro-CT imaging, cryogenic transmission electron microscopy, optical second harmonic generation, hinging and twisting motion measurements with a laser Doppler ..
  8. Phillip Darwin Bell; Fiscal Year: 2016
    ..Light and electron microscopy will be used to study cystic development and assess pathological changes in kidney and other organs and in ..
  9. MELINDA ANN FRYE; Fiscal Year: 2014
    ..Additional resources will allow HPLC, mass spectrometry, electron microscopy, genomic analyses, advanced immunohistochemistry studies and MRI. Drs...
  10. Pilot Studies of the Effect of Aging on Mucociliary Clearance
    Lawrence E Ostrowski; Fiscal Year: 2013
    ..will be determined by histology, and the incidence of axonemal structural defects will be quantified by electron microscopy. 2b) To test the hypothesis that aged mice exhibit defects in the mucus component of MCC...
  11. Neuropeptide regulation of neurohypophyseal function
    Francis W Flynn; Fiscal Year: 2012
    ..were shown to be translocated from the cell membrane to the cell nucleus by confocal microscopy, immuno-electron microscopy and Western blot. Nuclear NK3R was not detected under basal conditions...
  12. Dual-Receptor Targeted Nanoparticles for Photodynamic Therapy of Brain Cancer
    Ann Marie Broome; Fiscal Year: 2013
    ..silver enhancement immunohistochemistry, real time confocal laser scanning microscopy, and transmission electron microscopy. The cells will then be subjected to PDT, and cellular viability will be assessed using the MTT assay...
  13. SAMUEL BRANDON STEPHENS; Fiscal Year: 2015
    ..e. insulin secretion) ultrastructural characterization of insulin defects in my model va the Duke Electron Microscopy Core facility will have great impact on my knowledge and skill set as an islet cell biologist. Drs...
  14. Amelioration of cisplatin ototoxicity by transplatin
    Debashree Mukherjea; Fiscal Year: 2010
    ..responses (ABRs) and by assessing the morphology of cochlear inner and outer hair cells, using scanning electron microscopy (SEM)...
  15. Michael J Brenner; Fiscal Year: 2016
    ..Assessments will be made by scanning electron microscopy, auditory brainstem response testing, and immunohistochemical assays...
  16. Nitric Oxide Signaling with Heme Proteins
    William R Montfort; Fiscal Year: 2012
    ..Experiments involving X-ray crystallography, electron microscopy and mutagenesis are proposed. Specific Aim 2 is to understand mechanism in the allosteric regulation of sGC...
  17. Carol L Wells; Fiscal Year: 2014
    ..Experimental methods include standard microbiological methods, confocal microscopy and high resolution electron microscopy, cell sorting, fluorescence in situ hybridization, and biochemical analyses...
  18. MOLECULAR BIOLOGY OF HEMORRHAGIC SHOCK
    Timothy R Billiar; Fiscal Year: 2013
    ..expertise in state-of-the-art tissue imaging, including immunohistochemistry, confocal microscopy, electron microscopy, quantitative morphology, and in situ hybridization...
  19. Connexins in Neuronal and Glial Gap Junctions in the Central Nervous System
    John E Rash; Fiscal Year: 2012
    ..immunofluorescence microscopy with newly-developed freeze-fracture replica immunogold labeling (FRIL) electron microscopy to detect, quantify, determine the protein composition of, and measure the sizes of gap junctions ..
  20. KEVIN P CURRIE; Fiscal Year: 2014
    ..We will also use electron microscopy (EM) to assess the number, size, and distribution of large dense core vesicles...
  21. Novel PPAR-Independent Peroxisome Proliferators for Type 2 Diabetes
    LIDIA NIEROBISZ; Fiscal Year: 2011
    ..drug discovery pipeline targeting peroxisomal phenotypic endpoints without requiring laborious electron microscopy or histology at the screening stage...
  22. William M Nauseef; Fiscal Year: 2014
    ..Preliminary studies, using electron microscopy, indicate and confirm that a CLM surrounds F. tularensis...
  23. JONATHAN NOAH THON; Fiscal Year: 2014
    ..has added to my repertoire a number of specialized cell biology techniques including fluorescence and electron microscopy, live cell imaging, retroviral infection, cell culture, and transgenic mouse models to study PLT production...
  24. Detection of engineered nanomaterials in drinking water, food, commercial product
    Paul K Westerhoff; Fiscal Year: 2010
    ..Risk assessments from these exposures are hampered by the lack of adequate detection capabilities. While electron microscopy and other techniques can image NMs in samples, they fall short of being able to quantify the size, number ..
  25. Gang Ren; Fiscal Year: 2016
    ..b>Electron microscopy (EM), as a novel technique, allows direct visualization of individual particles...
  26. Structural mechanism of DNA segregation by the pSK41 par system
    Maria Schumacher; Fiscal Year: 2010
    ..will also elucidate the structure of the ParM filament and examine its interaction with the segrosome using electron microscopy, biochemistry and cellular studies. Importantly, pSK41, harbored in S...
  27. Thiol Isomerases During Thrombus Formation
    Barbara C Furie; Fiscal Year: 2013
    ..the cell surface or internal organelles, or both, by flow cytometry, immunofluorescence microscopy and electron microscopy. The redistribution of thiol isomerases during platelet activation and endothelial cell stimulation, with ..
  28. Microvascular Dysfunction in Hyperhomocysteinemia
    Shawn E Bearden; Fiscal Year: 2012
    ..b>Electron microscopy shows significant changes in the morphology of myoendothelial junctions in skeletal muscle arterioles...
  29. Kenneth R Johnson; Fiscal Year: 2016
    ..Inner ears of mutant mice at various ages will be examined by light and electron microscopy for structural anomalies, and temporal and spatial distributions of gene transcripts and proteins will be ..
  30. Pharmacology of Antiretroviral Nanoparticle Micelles
    Christopher J Destache; Fiscal Year: 2010
    ..Penetration into macrophages will be assessed by liquid chromatography and confocal and transmission electron microscopy. The nanoformulations will be used as a drug delivery system to improve tissue penetrance of ART into the ..
  31. Timothy L Cover; Fiscal Year: 2016
    ..Methods will include cryo-electron microscopy, crystallography, molecular genetics, and analysis of gene trap and shRNA libraries...
  32. Differentiation of Cord Blood Mesenchymal Stem Cells to Corneal Endothelium
    Nancy C Joyce; Fiscal Year: 2010
    ..Immunostaining for Ki67 and ZO-1 will test for contact inhibition of proliferation. Transmission electron microscopy will examine cellular morphology...
  33. Role of Biophysical Cues in the Pathogenesis of Glaucoma
    Paul Russell; Fiscal Year: 2013
    ..It has been reported that basement membrane is changed in glaucoma with a denser matrix observed with electron microscopy. The overall goal of this proposal is to 1...
  34. Barry M Willardson; Fiscal Year: 2016
    ..pulse-chase assembly measurements of protein complexes, purification of large protein complexes and cryo-electron microscopy. A strong team of collaborators has been assembled to provide the necessary expertise to successfully ..
  35. Autophagy and human islet amyloid polypeptide in animal models of type 2 diabetes
    Jacqueline Rivera; Fiscal Year: 2013
    ..e. LC3II, p62, and Atg7) and use of the gold standard in the field, electron microscopy. In Specific Aim Two, we will test the postulate that autophagy provides protection against formation of ..
  36. ClC-3 ion transport modulates NADPH oxidase-dependent PMN priming by endotoxin
    Jessica G Moreland; Fiscal Year: 2012
    ..Confocal and electron microscopy studies will define the localization and kinetics of oxidant generation by priming stimuli, and explore the ..
  37. Victor L Arvanian; Fiscal Year: 2016
    ..field and conduct a multidisciplinary examination, including in vivo physiology, anatomy, immunochemistry, electron microscopy and behavior...
  38. DEVELOPMENT AND MAINTENANCE OF LENS TRANSPARENCY
    JOHN IRWIN CLARK; Fiscal Year: 2013
    ..obtained using laser capture microdissection (LCM) will be combined with immunocytochemistry, and light and electron microscopy to correlate protein constituents with structural modifications accounting for the phenotype observed in ..
  39. 3D Differentiation of Mesenchymal Stem Cells in Peptide Amphiphile Matrices
    John B Matson; Fiscal Year: 2011
    ..Characterization of the gel will be accomplished using electron microscopy, small-angle X-ray scattering, rheology, and circular dichroism...
  40. A Spontaneous Mouse Model of Motor Neuron Disease
    Kevin L Seburn; Fiscal Year: 2011
    ..of gross motor performance, electrophysiological characterization, and histology, immunocytochemistry, and electron microscopy to understand the disease progression and pathology in the motor neurons...
  41. Energy Restrction and Mammary Cancer
    Henry J Thompson; Fiscal Year: 2013
    ..Methods will include transmission electron microscopy, RT-PCR, western blots, reverse phase protein arrays, IHC, and activity assays using fluorescent ..
  42. REACTIVE OXYGEN SPECIES AND AGING
    Elias K Michaelis; Fiscal Year: 2012
    ..b>Electron microscopy and quantitative imaging were added to Core C...
  43. SK channels: Roles and mechanisms for dendritic excitability and plasticity
    James G Maylie; Fiscal Year: 2012
    ..We will test these hypotheses by using an integrated, diverse technical repertoire including immuno electron microscopy, electrophysiology, SK transgenic mice, heterologous co-expression studies, and western and biochemical ..
  44. Frances M Brodsky; Fiscal Year: 2016
    ..The experiments proposed involve protein biochemistry, electron microscopy, in vitro cell biology, and in silico genetic analysis...
  45. PROTEIN KINASE C IN THE LENS
    DOLORES JEAN TAKEMOTO; Fiscal Year: 2010
    ..The approach includes biochemical studies, mass spectrometry (MS) analyses, structural studies using electron microscopy, and gating/impedance measurements on both cells in culture and whole lens. The Specific Aims are: 1...
  46. Heidi S Fisher; Fiscal Year: 2014
    ..focus will be on exploring sperm aggregation behavior to understand both how groups form using integrative electron microscopy and why they do - by asking what is the effect of cooperative sperm migration within the female ..
  47. Autophagy in epidermal melanocyte: a protective or a destructive role?
    Vijayasaradhi Setaluri; Fiscal Year: 2013
    ..induces autophagy, which can be monitored by morphological (light microscopic immunofluorescence and electron microscopy) and biochemical methods...
  48. The influenza A virus nucleoprotein: structure and function
    Yizhi Jane Tao; Fiscal Year: 2012
    ..Our proposed research employs a broad spectrum of X-ray crystallography, electron microscopy and other biophysical techniques (by Dr. Tao, PI) together with functional and virological methods (by Dr...
  49. Kristen M Harris; Fiscal Year: 2015
    ..The approach is three-dimensional reconstruction and quantification through serial section electron microscopy (EM)...
  50. ALEXIS JOANNA SLOAN; Fiscal Year: 2014
    ..within fours days of doxycycline exposure and display foot process effacement upon analysis with electron microscopy. Our preliminary data also show that animals on prolonged doxycycline exposure (2 months) are unable to ..
  51. Mitochondrial chaperones mortalin and Tid1 in protein degradation
    Carolyn K Suzuki; Fiscal Year: 2012
    ..b>Electron microscopy data show the accumulation of inclusion bodies within mitochondria of Lon-depleted cells...
  52. Maria Medalla; Fiscal Year: 2014
    ..which recordings are obtained will be conducted using high-resolution laser-scanning confocal imaging and electron microscopy of filled dendrites...
  53. Richard J Kuhn; Fiscal Year: 2014
    ..protein and the envelope glycoproteins will be used in conjunction with pseudo-atomic resolution cryo-electron microscopy image reconstructions as a guide for the molecular genetic analysis of the nucleocapsid core and envelope ..
  54. Interactions between P. aeruginosa and autophagy in Airway epithelial cells
    INDHIRA H DE LA ROSA; Fiscal Year: 2013
    ..assay, as well as by evaluating their colocalization with autophagy membranes using both fluorescence and electron microscopy. (b) Investigate whether P...
  55. Kim A Brogden; Fiscal Year: 2014
    ..The inhibition of rHagB or rFimA binding to cells by HBD3 will be determined by confocal microscopy and electron microscopy. The identity of rHagB and rFimA receptors on cell membranes will be identified using two-dimensional ..
  56. Edward H Egelman; Fiscal Year: 2016
    ..The main tools that we have been using are electron microscopy and computational image analysis...
  57. Antiviral activity of leflunomide against respiratory syncytial virus
    WILLIAM WALDMAN; Fiscal Year: 2009
    ..and western blot analysis of viral protein synthesis and phosphorylation, and immunogold electron microscopy. Data generated by these studies are anticipated to demonstrate the bifunctional utility of leflunomide in ..
  58. Lisa Ostrin; Fiscal Year: 2016
    ..will be complemented by ultrastructural studies of the lamina cribrosa of the deep optic nerve head, using electron microscopy. To specifically assess whether myopic eyes are more susceptible to glaucoma, IOP will be elevated in ..
  59. Regeneration of stereocilia of cochlear hair cells
    David Z Z He; Fiscal Year: 2010
    ..will be examined using immunohistochemistry (with confocal microscopy), time-lapse imaging, scanning electron microscopy, and electrophysiology...
  60. THOMAS N WICHMANN; Fiscal Year: 2015
    ..Smith) which will provide immunohistochemistry and electron microscopy services to all of the center projects, and will conduct behavioral testing in MPTP-treated monkeys under ..
  61. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2010
    ..Several recent findings from this laboratory reveal the power of serial section transmission electron microscopy (ssTEM) to investigate these relationships. Dendrites containing more microtubules had more synapses...
  62. Legionella pneumophila developmental cycle & virulence
    PAUL STOKES HOFFMAN; Fiscal Year: 2011
    ..conditions that promote cyst morphogenesis with sub aims of (i) tracking morphogenesis by light and electron microscopy;(i) by reporter gene fusions;(iii) pulse chase radiolabeling and proteome profiling and (iv)to assess cyst ..
  63. Base-selective heavy atom labels for electron microscopy-based DNA sequencing
    F D Toste; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Base-selective heavy atom labels for electron microscopy-based DNA sequencing Project Summary/Abstract The development of inexpensive and rapid DNA sequencing technology remains a major challenge of ..
  64. Three-Dimensional Imaging of Whole Cells by Using X-ray Diffraction Microscopy
    Jianwei Miao; Fiscal Year: 2010
    ..requires employment of different techniques and methods such as nuclear magnetic resonance and cryo-electron microscopy. A very promising approach currently under rapid development is X-ray diffraction microscopy (i.e...
  65. METHOTREXATE EFFECTS OF ASTROCYTES
    Jocelyn Bruce; Fiscal Year: 1992
    ..For in vivo studies, brain sections from Sprague-Dawley rats will be examined by light and electron microscopy at various times following intraperitoneal injection with varying doses of MTX...
  66. A feasibility study of genetically encoded fluorescent gold nanoclusters
    Jan Liphardt; Fiscal Year: 2009
    ..Potential adverse effects will be assessed by optical and electron microscopy. 2...
  67. SUBCELLULAR EFFECTS OF METABOLIC HORMONES ON RAT LIVER
    ROBERT CARDELL; Fiscal Year: 1991
    ..information related to subcellular mechanisms of hepatic glycogen metabolism through the use of light and electron microscopy autoradiographs and immunocytochemistry using colloidal gold and cDNA probes...