confocal microscopy

Summary

Summary: A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.

Top Publications

  1. ncbi A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
  2. pmc Ultrafine particles cross cellular membranes by nonphagocytic mechanisms in lungs and in cultured cells
    Marianne Geiser
    Institute for Anatomy, University of Bern, Bern, Switzerland
    Environ Health Perspect 113:1555-60. 2005
  3. ncbi Assessment of human pancreatic islet architecture and composition by laser scanning confocal microscopy
    Marcela Brissova
    Department of Medicine, Division of Diabetes, Endocrinology, and Metabolism, 715 PRB, Vanderbilt University, Nashville, TN 37232, USA
    J Histochem Cytochem 53:1087-97. 2005
  4. pmc Megabase chromatin domains involved in DNA double-strand breaks in vivo
    E P Rogakou
    Laboratory of Molecular Pharmacology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 146:905-16. 1999
  5. pmc Interpreting second-harmonic generation images of collagen I fibrils
    Rebecca M Williams
    Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Biophys J 88:1377-86. 2005
  6. pmc Measuring fast dynamics in solutions and cells with a laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Illinois, USA
    Biophys J 89:1317-27. 2005
  7. pmc Ex vivo dynamic imaging of retinal microglia using time-lapse confocal microscopy
    Jung Eun Lee
    Office of the Scientific Director, National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA
    Invest Ophthalmol Vis Sci 49:4169-76. 2008
  8. ncbi Evaluation of atlas selection strategies for atlas-based image segmentation with application to confocal microscopy images of bee brains
    Torsten Rohlfing
    Image Guidance Laboratories, Department of Neurosurgery, Stanford University, Stanford, CA 94305 5327, USA
    Neuroimage 21:1428-42. 2004
  9. ncbi Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation
    Edward Brown
    Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Nat Med 9:796-800. 2003
  10. pmc Hypertrophic versus non hypertrophic scars compared by immunohistochemistry and laser confocal microscopy: type I and III collagens
    Gisele V Oliveira
    Shriners Burns Hospital, Galveston, TX, USA
    Int Wound J 6:445-52. 2009

Detail Information

Publications328 found, 100 shown here

  1. ncbi A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
    ..With the improved speed and efficiency of maturation and the increased resistance to environment, Venus will enable fluorescent labelings that were not possible before...
  2. pmc Ultrafine particles cross cellular membranes by nonphagocytic mechanisms in lungs and in cultured cells
    Marianne Geiser
    Institute for Anatomy, University of Bern, Bern, Switzerland
    Environ Health Perspect 113:1555-60. 2005
    ..Particles within cells are not membrane bound and hence have direct access to intracellular proteins, organelles, and DNA, which may greatly enhance their toxic potential...
  3. ncbi Assessment of human pancreatic islet architecture and composition by laser scanning confocal microscopy
    Marcela Brissova
    Department of Medicine, Division of Diabetes, Endocrinology, and Metabolism, 715 PRB, Vanderbilt University, Nashville, TN 37232, USA
    J Histochem Cytochem 53:1087-97. 2005
    ..The results indicate that human islets not only are quite heterogeneous in terms of cell composition but also have a substantially different architecture from widely studied murine islets...
  4. pmc Megabase chromatin domains involved in DNA double-strand breaks in vivo
    E P Rogakou
    Laboratory of Molecular Pharmacology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 146:905-16. 1999
    ..The results further suggest the possible existence of units of higher order chromatin structure involved in monitoring DNA integrity...
  5. pmc Interpreting second-harmonic generation images of collagen I fibrils
    Rebecca M Williams
    Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Biophys J 88:1377-86. 2005
    ..Fibrillogenesis can be resolved in immature tissue by directly imaging backward-propagating SHG. Such findings are crucial to the design and development of forthcoming diagnostic and research tools...
  6. pmc Measuring fast dynamics in solutions and cells with a laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Illinois, USA
    Biophys J 89:1317-27. 2005
    ..This new type of data analysis has a broad application in biology and it provides a powerful tool for measuring fast as well as slower dynamic processes in cellular systems using any standard laser confocal microscope...
  7. pmc Ex vivo dynamic imaging of retinal microglia using time-lapse confocal microscopy
    Jung Eun Lee
    Office of the Scientific Director, National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA
    Invest Ophthalmol Vis Sci 49:4169-76. 2008
    ..Here, the authors used a novel ex vivo retinal imaging preparation to examine the behavioral phenotype of living retinal microglia in intact tissue and in response to injury...
  8. ncbi Evaluation of atlas selection strategies for atlas-based image segmentation with application to confocal microscopy images of bee brains
    Torsten Rohlfing
    Image Guidance Laboratories, Department of Neurosurgery, Stanford University, Stanford, CA 94305 5327, USA
    Neuroimage 21:1428-42. 2004
    ..Segmentation by intensity-based nonrigid registration to atlas images is applied to confocal microscopy images acquired from the brains of 20 bees...
  9. ncbi Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation
    Edward Brown
    Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Nat Med 9:796-800. 2003
    ..This technology could offer basic scientists and clinicians an enhanced ability to estimate the relative penetrabilities of molecular therapeutics...
  10. pmc Hypertrophic versus non hypertrophic scars compared by immunohistochemistry and laser confocal microscopy: type I and III collagens
    Gisele V Oliveira
    Shriners Burns Hospital, Galveston, TX, USA
    Int Wound J 6:445-52. 2009
    ..Easily accessed by immunohistochemistry and confocal microscopy, type III collagen deposition may help in determining scar phenotype, differentiating hypertrophic and non ..
  11. ncbi Quantification of dental microwear by tandem scanning confocal microscopy and scale-sensitive fractal analyses
    Peter S Ungar
    Department of Anthropology, University of Arkansas, Fayetteville, Arkansas 72701, USA
    Scanning 25:185-93. 2003
    ..This paper describes a new approach to the analysis of dental microwear using tandem scanning confocal microscopy and scale-sensitive fractal analyses...
  12. pmc Quantitative determination of spatial protein-protein correlations in fluorescence confocal microscopy
    Yong Wu
    Department of Anesthesiology, Division of Molecular Medicine, University of California at Los Angeles, California, USA
    Biophys J 98:493-504. 2010
    ..We also employed the median-filter method as a universal approach for background reduction to minimize nonspecific fluorescence. We illustrated our method by analyzing computer-simulated images and biological images...
  13. ncbi Localization of the permeability barrier to solutes in isolated arteries by confocal microscopy
    Paul M A van Haaren
    Department of Medical Physics, Cardiovascular Research Institute Amsterdam, Academic Medical Center, University of Amsterdam, 1100 DE Amsterdam, The Netherlands
    Am J Physiol Heart Circ Physiol 285:H2848-56. 2003
    ..by FITC-labeled dextrans (FITC-Deltas) and the endothelial membrane dye DiI were determined with confocal microscopy. Large FITC-Delta (148 kDa) filled a core volume inside the arteries within 1 min but was excluded from a 2...
  14. ncbi Calreticulin inhibits repetitive intracellular Ca2+ waves
    P Camacho
    Department of Neuroscience, University of Virginia Health Sciences Center, Charlottesville 22908, USA
    Cell 82:765-71. 1995
    ..This novel function of CRT in intracellular Ca2+ signaling may be regulated by Ca2+ occupancy of the high affinity binding site...
  15. ncbi The glucocorticoid receptor: rapid exchange with regulatory sites in living cells
    J G McNally
    Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, National Cancer Institute, Bethesda, MD 20892 5055, USA
    Science 287:1262-5. 2000
    ..Thus, the interaction of regulatory proteins with target sites in chromatin is a more dynamic process than previously believed...
  16. ncbi Intracellular localization and trafficking of fluorescein-labeled cisplatin in human ovarian carcinoma cells
    Roohangiz Safaei
    Department of Medicine, and the Rebecca and John Moores Cancer Center, University of California San Diego, San Diego, CA 92093, USA
    Clin Cancer Res 11:756-67. 2005
    ..We sought to identify the subcellular compartments in which cisplatin [cis-diamminedichloroplatinum (DDP)] accumulates in human ovarian carcinoma cells and define its export pathways...
  17. pmc Endocytic pathways: combined scanning ion conductance and surface confocal microscopy study
    Andrew I Shevchuk
    MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK
    Pflugers Arch 456:227-35. 2008
    We introduce a novel high resolution scanning surface confocal microscopy technique that enables imaging of endocytic pits in apical membranes of live cells for the first time...
  18. pmc Robust pore size analysis of filamentous networks from three-dimensional confocal microscopy
    Walter Mickel
    Institut fur Theoretische Physik, Friedrich Alexander Universitat Erlangen Nurnberg, Erlangen, Germany
    Biophys J 95:6072-80. 2008
    ..properties of filamentous biopolymer networks, such as collagen or other connective tissue matrices, from confocal microscopy image stacks...
  19. ncbi A complex and mobile structure forms a distinct subregion within the continuous vacuolar membrane in young cotyledons of Arabidopsis
    Chieko Saito
    Molecular Membrane Biology Laboratory, RIKEN, Wako, Saitama 351 0198, Japan
    Plant J 29:245-55. 2002
    ..These results suggest that bulbs define a subregion in the continuous vacuolar membrane, where some proteins are concentrated and others segregated...
  20. doi Functional entry of dengue virus into Aedes albopictus mosquito cells is dependent on clathrin-mediated endocytosis
    Eliana G Acosta
    Laboratorio de Virologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
    J Gen Virol 89:474-84. 2008
    ..Using confocal microscopy, co-localization of DENV-2 E glycoprotein and the marker protein transferrin was observed at the periphery ..
  21. ncbi Genetic and cellular analyses of zebrafish atrioventricular cushion and valve development
    Dimitris Beis
    Department of Biochemistry and Biophysics and Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    Development 132:4193-204. 2005
    ..This collection of mutants provides a unique set of tools to further our understanding of the genetic basis of cell behavior and differentiation during AV valve development...
  22. ncbi Rapid automated three-dimensional tracing of neurons from confocal image stacks
    Khalid A Al-Kofahi
    Electrical, Computer, and Systems Engineering Department, Rensselaer Polytechnic Institute, Troy, NY 12180 3590, USA
    IEEE Trans Inf Technol Biomed 6:171-87. 2002
    ..presented for fully automatic three-dimensional (3-D) tracing of neurons that are imaged by fluorescence confocal microscopy. Unlike previous voxel-based skeletonization methods, the present approach works by recursively following ..
  23. ncbi Overexpression of vascular endothelial growth factor 165 drives peritumor interstitial convection and induces lymphatic drain: magnetic resonance imaging, confocal microscopy, and histological tracking of triple-labeled albumin
    Hagit Dafni
    Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100 Israel
    Cancer Res 62:6731-9. 2002
    ..This contrast material allowed for multimodality imaging using magnetic resonance imaging (MRI), confocal microscopy, and histology...
  24. pmc Regulatory T cells inhibit stable contacts between CD4+ T cells and dendritic cells in vivo
    Carlos E Tadokoro
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, and Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    J Exp Med 203:505-11. 2006
    ..Thus, T reg cells can exert an early effect on immune responses by attenuating the establishment of stable contacts during priming of naive T cells by DCs...
  25. doi Corneal sensitivity and slit scanning in vivo confocal microscopy of the subbasal nerve plexus of the normal central and peripheral human cornea
    Dipika V Patel
    Department of Ophthalmology, Faculty of Medical and Health Science, University of Auckland, Auckland, New Zealand 1142
    Cornea 28:735-40. 2009
    ..To determine the subbasal nerve density and tortuosity at 5 corneal locations and to investigate whether these microstructural observations correlate with corneal sensitivity...
  26. pmc Common themes and cell type specific variations of higher order chromatin arrangements in the mouse
    Robert Mayer
    Department Biologie II, Ludwig Maximilians Universitat Munchen, Grosshaderner Str 2, 82152 Planegg Martinsried, Germany
    BMC Cell Biol 6:44. 2005
    ....
  27. doi A morphological analysis of growth cones of DRG neurons combining atomic force and confocal microscopy
    Jummi Laishram
    International School for Advanced Studies SISSA ISAS, Trieste, Italy
    J Struct Biol 168:366-77. 2009
    ..growth cones of differentiating neurons from rat dorsal root ganglia (DRG) with conventional Laser Scanning Confocal Microscopy (LSCM) and Atomic Force Microscopy (AFM)...
  28. ncbi Environment-specific expression of the immediate-early gene Arc in hippocampal neuronal ensembles
    J F Guzowski
    Virion Systems, Inc, 9610 Medical Center Drive, Suite 100, Rockville, Maryland 20850, USA
    Nat Neurosci 2:1120-4. 1999
    We used fluorescent in-situ hybridization and confocal microscopy to monitor the subcellular distribution of the immediate-early gene Arc...
  29. doi Reflectance confocal microscopy for pigmentary disorders
    Hee Young Kang
    Department of Dermatology, Ajou University School of Medicine, Suwon, Korea
    Exp Dermatol 19:233-9. 2010
    In vivo reflectance confocal microscopy (RCM) is a non-invasive, repetitive imaging tool that provides real-time images at nearly cellular histological resolution...
  30. doi In vivo and in vitro laser confocal microscopy to diagnose acanthamoeba keratitis
    Atsushi Shiraishi
    Department of Ophthalmology and Regenerative Medicine, Ehime University Graduate School of Medicine, Toon, Ehime, Japan
    Cornea 29:861-5. 2010
    To determine the effectiveness of laser confocal microscopy in identifying Acanthamoeba cysts and trophozoites in the cornea of patients with Acanthamoeba keratitis (AK) and to evaluate its effectiveness in following AK after treatment.
  31. doi In vivo laser confocal microscopy after non-Descemet's stripping automated endothelial keratoplasty
    Akira Kobayashi
    Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan
    Ophthalmology 116:1306-13. 2009
    To investigate in vivo corneal changes in patients with bullous keratopathy who underwent non-Descemet's stripping automated endothelial keratoplasty (nDSAEK) with the use of laser confocal microscopy.
  32. doi Live conjunctiva-associated lymphoid tissue analysis in rabbit under inflammatory stimuli using in vivo confocal microscopy
    Hong Liang
    Department of Toxicology, Faculty of Biological and Pharmacological Sciences, Paris Descartes University, Paris, France
    Invest Ophthalmol Vis Sci 51:1008-15. 2010
    ..The authors investigated in rabbit eyes the cell reactions occurring in the organized CALT during conjunctival inflammation models...
  33. ncbi Dendritic cells are early cellular targets of Listeria monocytogenes after intestinal delivery and are involved in bacterial spread in the host
    B Pron
    , 75015 Paris, France
    Cell Microbiol 3:331-40. 2001
    ..to the dissemination of Listeria monocytogenes from the gut to draining mesenteric lymph nodes (MLNs) by confocal microscopy of immunostained tissue sections from a rat ligated ileal loop system...
  34. doi Volume reconstruction of large tissue specimens from serial physical sections using confocal microscopy and correction of cutting deformations by elastic registration
    Martin Capek
    Department of Biomathematics, Institute of Physiology, Academy of Sciences of the Czech Republic, v v i, Videnska 1083, 142 20 Prague 4 Krc, Czech Republic
    Microsc Res Tech 72:110-9. 2009
    ..In this work, we demonstrate the reconstruction using a postcranial part of a 17-day-old laboratory Wistar rat embryo...
  35. pmc Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy
    Partha Mukhopadhyay
    Section on Oxidative Stress Tissue Injury, Laboratories of Physiological Studies, National Institutes of Health NIAAA, Bethesda, Maryland 20892, USA
    Nat Protoc 2:2295-301. 2007
    ..introduced for selective detection of superoxide in the mitochondria of live cells and was validated for confocal microscopy and flow cytometry...
  36. pmc Cellular and subcellular localization of endogenous nitric oxide in young and senescent pea plants
    Francisco J Corpas
    Departamento de Bioquimica, Biologia Celular y Molecular de Plantas, Consejo Superior de Investigaciones Cientificas, E 18080 Granada, Spain
    Plant Physiol 136:2722-33. 2004
    ..In senescent pea leaves, the NOS-like activity of peroxisomes was down-regulated by 72%. It is proposed that peroxisomal NO. could be involved in the process of senescence of pea leaves...
  37. ncbi The expression pattern of the Drosophila vesicular glutamate transporter: a marker protein for motoneurons and glutamatergic centers in the brain
    Andrea Mahr
    Max Planck Institut fur Entwicklungsbiologie, Abt III Genetik, Spemannstr 35, 72076 Tubingen, Germany
    Gene Expr Patterns 6:299-309. 2006
    ..Given that vesicular glutamate transporters are essential for the uptake of the neurotransmitter glutamate into synaptic vesicles these tools provide a means to test gene function in these functionally important neurons...
  38. ncbi The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth
    Niko Geldner
    ZMBP, Entwicklungsgenetik, Universitat Tubingen, Auf der Morgenstelle 3, D 72076, Tubingen, Germany
    Cell 112:219-30. 2003
    ..Our results demonstrate that GNOM is required for the recycling of auxin transport components and suggest that ARF-GEFs regulate specific endosomal trafficking pathways...
  39. pmc Matrilysin (matrix metalloproteinase-7) mediates E-cadherin ectodomain shedding in injured lung epithelium
    John K McGuire
    Department of Pediatrics, Washington University School of Medicine, St Louis, USA
    Am J Pathol 162:1831-43. 2003
    ..These findings identify E-cadherin as a novel substrate for matrilysin and indicate that shedding of E-cadherin ectodomain is required for epithelial repair...
  40. ncbi mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane of differentiated myocytes
    M S Kapiloff
    Howard Hughes Medical Institute, L 474, Vollum Institute, Portland OR 97201 3098, USA
    J Cell Sci 112:2725-36. 1999
    ....
  41. pmc HIV binding, penetration, and primary infection in human cervicovaginal tissue
    Diane Maher
    Department of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware Street Southeast, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 102:11504-9. 2005
    ..b>Confocal microscopy combined with 3D surface reconstruction revealed that virions could both bind to the external surface of ..
  42. pmc Mapping eGFP oligomer mobility in living cell nuclei
    Nicolas Dross
    Division Biophysics of Macromolecules, German Cancer Research Center DKFZ, Heidelberg, Germany
    PLoS ONE 4:e5041. 2009
    ..Even the densest chromatin regions do not exclude free eGFP-monomers or multimers...
  43. ncbi Endocytic delivery to lysosomes mediated by concurrent fusion and kissing events in living cells
    Nicholas A Bright
    Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2XY, United Kingdom
    Curr Biol 15:360-5. 2005
    ..We utilized time-lapse confocal microscopy to test these hypotheses in living cells...
  44. ncbi Increased expression of the copper efflux transporter ATP7A mediates resistance to cisplatin, carboplatin, and oxaliplatin in ovarian cancer cells
    Goli Samimi
    Department of Medicine, University of California San Diego, La Jolla, California, 92093 0058, USA
    Clin Cancer Res 10:4661-9. 2004
    ..The goal of this study was to determine the effect of small changes in ATP7A expression on the pharmacodynamics of cisplatin, carboplatin, and oxaliplatin in human ovarian carcinoma cells...
  45. pmc A near-infrared cell tracker reagent for multiscopic in vivo imaging and quantification of leukocyte immune responses
    Filip K Swirski
    Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts, United States of America fswirski mgh harvard edu
    PLoS ONE 2:e1075. 2007
    ..Thus, this approach is suitable to monitor cells at multiple resolutions in real time in their native environments by NIR-based fluorescence imaging...
  46. pmc Spatial patterns of DNA replication, protein synthesis, and oxygen concentration within bacterial biofilms reveal diverse physiological states
    Suriani Abdul Rani
    Center for Biofilm Engineering, Montana State University Bozeman, Bozeman, MT 59717 3980, USA
    J Bacteriol 189:4223-33. 2007
    ..The variety of activity states represented in a biofilm may contribute to the special ecology and tolerance to antimicrobial agents of biofilms...
  47. pmc Peroxisomes are required for in vivo nitric oxide accumulation in the cytosol following salinity stress of Arabidopsis plants
    Francisco J Corpas
    Departamento de Bioquimica, Biologia Celular y Molecular de Plantas, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, E 18080 Granada, Spain
    Plant Physiol 151:2083-94. 2009
    ....
  48. pmc Helicobacter pylori resists phagocytosis by macrophages: quantitative assessment by confocal microscopy and fluorescence-activated cell sorting
    N Ramarao
    , Abteilung Molekulare Biologie, 10117 Berlin, Germany
    Infect Immun 69:2604-11. 2001
    ..pylori and phagocytes. Our data were obtained using laser confocal microscopy and flow cytometry after quenching the fluorescence of labeled extracellular bacteria...
  49. pmc Munc18-1 is critical for plasma membrane localization of syntaxin1 but not of SNAP-25 in PC12 cells
    Lakshmanan Arunachalam
    Division of Fundamental Neurobiology, Toronto Western Research Institute, University Health Network, Toronto, Ontario, M5T 2S8, Canada
    Mol Biol Cell 19:722-34. 2008
    ....
  50. ncbi Molecular cloning and characterization of a novel gene family of four ancient conserved domain proteins (ACDP)
    Cong Yi Wang
    Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta, GA 30912, USA
    Gene 306:37-44. 2003
    ..Sequence homology analyses revealed AA property and structural homologies between the ACD domain and cyclin molecules...
  51. pmc Gamma-tubulin is required for the structure and function of the microtubule organizing centre in Drosophila neuroblasts
    C E Sunkel
    Centro de Citologia Experimental, Universidade do Porto, Portugal
    EMBO J 14:28-36. 1995
    ..These results show that gamma-tubulin is required for both structural and functional roles in the MTOCs...
  52. ncbi Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling
    Weigang Wang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 62:6278-88. 2002
    ..We conclude that aligning cell behavior in vivo with patterns of gene expression can lead to new insights into the microenvironment of carcinoma cells in the primary tumor and the molecular mechanisms behind cell behavior...
  53. pmc Evidence for the involvement of VAR2CSA in pregnancy-associated malaria
    Ali Salanti
    Centre for Medical Parasitology, Dept of Medical Microbiology and Immunology, Panum Institute 24 2, Blegdamsvej 3, 2200 Copenhagen, Denmark
    J Exp Med 200:1197-203. 2004
    ....
  54. ncbi In vivo confocal microscopy study of blebs after filtering surgery
    Antoine Labbe
    Department of Ophthalmology III, Quinze Vingts National Ophthalmology Hospital, Paris, France
    Ophthalmology 112:1979. 2005
    ..To analyze bleb structure after filtering surgery at the cellular level using a new generation in vivo confocal microscope...
  55. pmc Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis
    Yang ja Lee
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 15:5001-11. 2004
    ..However, we provide further evidence that multiple components of the mitochondrial morphogenesis machinery can positively and negatively regulate apoptosis...
  56. pmc Structural interactions between kisspeptin and GnRH neurons in the mediobasal hypothalamus of the male rhesus monkey (Macaca mulatta) as revealed by double immunofluorescence and confocal microscopy
    Suresh Ramaswamy
    Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Endocrinology 149:4387-95. 2008
    ..fluorescently tagged secondary antibodies (antisheep, Alexa Fluor 488; antirabbit, Cy3) in combination with confocal microscopy. Kisspeptin perikarya were found only in the mediobasal hypothalamus (MBH) almost exclusively in the ..
  57. pmc RIN4 functions with plasma membrane H+-ATPases to regulate stomatal apertures during pathogen attack
    Jun Liu
    Department of Plant Pathology, University of California Davis, Davis, California, USA
    PLoS Biol 7:e1000139. 2009
    ..These results indicate that the Arabidopsis protein RIN4 functions with the PM H(+)-ATPase to regulate stomatal apertures, inhibiting the entry of bacterial pathogens into the plant leaf during infection...
  58. pmc Chlamydia trachomatis polymorphic membrane protein D is an oligomeric autotransporter with a higher-order structure
    Kena A Swanson
    Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th St, Hamilton, MT 59840, USA
    Infect Immun 77:508-16. 2009
    ..We hypothesize that PmpD is a multifunctional virulence factor important in chlamydial pathogenesis and could represent novel vaccine or drug targets for the control of human chlamydial infections...
  59. ncbi Digitizing life at the level of the cell: high-performance laser-scanning microscopy and image analysis for in toto imaging of development
    Sean G Megason
    Biological Imaging Center, Beckman Institute and Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Mech Dev 120:1407-20. 2003
    ..There are significant challenges that must be met to reach these goals. This paper will discuss the current state-of-the-art, the challenges, and the prospects of in toto imaging in the areas of imaging, image analysis, and informatics...
  60. doi A fundamental role for interleukin-21 in the generation of T follicular helper cells
    Alexis Vogelzang
    Department of Immunology, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, NSW 2010, Australia
    Immunity 29:127-37. 2008
    ..This study reveals a previously unappreciated role for Tfh cells in the formation of the GC and isotype switching through a CD4(+) T cell-intrinsic requirement for IL-21...
  61. doi In vivo laser confocal microscopy after descemet stripping with automated endothelial keratoplasty
    Akira Kobayashi
    Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, 13 1 Takara machi, Kanazawa shi, Ishikawa Ken, Japan
    Am J Ophthalmol 145:977-985. 2008
    To investigate in vivo corneal changes in patients with bullous keratopathies before and after Descemet stripping with automated endothelial keratoplasty (DSAEK) using laser confocal microscopy.
  62. ncbi The antitumor ether lipid ET-18-OCH(3) induces apoptosis through translocation and capping of Fas/CD95 into membrane rafts in human leukemic cells
    C Gajate
    , , CSIC-Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, Spain
    Blood 98:3860-3. 2001
    ..This translocation of Fas into membrane rafts may provide a mechanism for amplifying Fas signaling by reorganization of membrane microdomains...
  63. ncbi Confocal microscopy in early diagnosis of Acanthamoeba keratitis
    Eliane Nakano
    Excimer Laser Santa Cruz, Sao Paulo, Brazil
    J Refract Surg 20:S737-40. 2004
    ..This study correlated confocal microscopic images obtained using the Nidek ConfoScan 2.0 System in corneas with clinical suspicion of Acanthamoeba keratitis, with diagnosis confirmed by either cytological and/or histological analysis...
  64. ncbi Cell proliferation without neurogenesis in adult primate neocortex
    D R Kornack
    Department of Neurobiology, Yale University School of Medicine, New Haven, CT 06510, USA
    Science 294:2127-30. 2001
    ..Thus, our results do not substantiate the claim of neurogenesis in normal adult primate neocortex...
  65. ncbi New techniques for imaging, digitization and analysis of three-dimensional neural morphology on multiple scales
    S L Wearne
    Computational Neurobiology and Imaging Center, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Neuroscience 136:661-80. 2005
    ....
  66. ncbi Overexpression of wild-type and mutant mucolipin proteins in mammalian cells: effects on the late endocytic compartment organization
    M Manzoni
    Sezione di Biologia e Genetica, Dipartimento di Scienze Biomediche e Biotecnologie, Viale Europa 11, 25123 Brescia, Italy
    FEBS Lett 567:219-24. 2004
    ..Among the mutants, only F408del induces a redistribution of the late endocytic compartment. These findings suggest that the overexpression of the mucolipin cation channel influences the dynamic equilibrium of late endocytic compartments...
  67. ncbi A new description of the reproductive system of Schistosoma mansoni (Trematoda: Schistosomatidae) analyzed by confocal laser scanning microscopy
    Renata Heisler Neves
    Disciplina de Parasitologia, Departamento de Patologia e Laboratorios, Faculdade de Ciencias Medicas, Universidade do Estado do Rio de Janeiro UERJ, Rua Prof Manuel de Abreu 444, 20551 031, Rio de Janeiro, RJ, Brazil
    Parasitol Res 95:43-9. 2005
    ..mansoni adult worms...
  68. ncbi Mammalian Smaug is a translational repressor that forms cytoplasmic foci similar to stress granules
    María V Baez
    Fundacion Instituto Leloir, IIBBA CONICET and IIB Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, C1405BWE Buenos Aires, Argentina
    J Biol Chem 280:43131-40. 2005
    ..Biochemical analysis indicated that mSmaug 1 is present in synaptoneurosomal 20 S particles. These results suggest a role for mammalian Smaug 1 in RNA granule formation and translation regulation in neurons...
  69. ncbi Fibroblast growth factor 1 regulates signaling via the glycogen synthase kinase-3beta pathway. Implications for neuroprotection
    Makoto Hashimoto
    Department of Neurosciences, University of California San Diego, La Jolla, California 92093 0624, USA
    J Biol Chem 277:32985-91. 2002
    ..Taken together these results suggest that neuroprotective effects of FGF1 might involve inactivation of GSK3beta by a pathway involving activation of the PI3K-Akt cascades...
  70. ncbi Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy
    Anne Sophie Nicot
    Institut de Genetique et de Biologie Moleculaire et Cellulaire IGBMC, Department of Molecular Pathology, F 67400 Illkirch, France
    Nat Genet 39:1134-9. 2007
    ....
  71. pmc Intermediate neuronal progenitors (basal progenitors) produce pyramidal-projection neurons for all layers of cerebral cortex
    Tom Kowalczyk
    Departments of Neurological Surgery and Pathology, University of Washington and Center for Neuroscience, Seattle Children s Hospital Research Institute, Seattle, WA 98101, USA
    Cereb Cortex 19:2439-50. 2009
    ..Our findings support the hypothesis that regulated amplification of INPs may be an important factor controlling the balance of neurogenesis among different cortical layers...
  72. doi Analysis of interactions among the CLAVATA3 receptors reveals a direct interaction between CLAVATA2 and CORYNE in Arabidopsis
    Yingfang Zhu
    Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100049, China
    Plant J 61:223-33. 2010
    ..Taken together, our results add direct evidence to the newly proposed two-parallel receptor pathways model and therefore provide new insights into the CLV3 signaling pathway...
  73. ncbi Compromised host defense on Pseudomonas aeruginosa biofilms: characterization of neutrophil and biofilm interactions
    Algirdas J Jesaitis
    Department of Microbiology, Montana State University, Bozeman, MT 59717 3520, USA
    J Immunol 171:4329-39. 2003
    ..Thus, host defense becomes compromised as biofilm bacteria escape while neutrophils remain immobilized with a diminished oxidative potential...
  74. doi An in vivo confocal microscopy analysis of effects of topical antiglaucoma therapy with preservative on corneal innervation and morphology
    Gianluca Martone
    Department of Ophthalmology, University of Siena, Siena, Italy
    Am J Ophthalmol 147:725-735.e1. 2009
    ..To evaluate the long-term effects of preservative-free and preservative-containing antiglaucoma eye drops on the tear secretion and ocular surface...
  75. pmc Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein
    Shaohui Huang
    School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA
    Biophys J 82:2811-25. 2002
    ..Finally, 2P-autofluorescence emission spectra of single cardiac cells have been obtained, with results suggesting potential for substantial improvement of the proposed 2P-ratiometric technique...
  76. ncbi Deep tissue two-photon microscopy
    Fritjof Helmchen
    Department of Neurophysiology, Brain Research Institute, University of Zurich, CH 8057 Zurich, Switzerland
    Nat Methods 2:932-40. 2005
    ..Here we review fundamental concepts of nonlinear microscopy and discuss conditions relevant for achieving large imaging depths in intact tissue...
  77. ncbi Light microscopy techniques for live cell imaging
    David J Stephens
    Department of Biochemistry, University of Bristol, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
    Science 300:82-6. 2003
    ..For anyone new to this area, however, it can be daunting to decide which techniques or equipment to try. Here, we aim to give a brief overview of the main approaches to live cell imaging, with some mention of their pros and cons...
  78. pmc Rab7: a key to lysosome biogenesis
    C Bucci
    Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen, Denmark
    Mol Biol Cell 11:467-80. 2000
    ..These data demonstrate for the first time that Rab7, controlling aggregation and fusion of late endocytic structures/lysosomes, is essential for maintenance of the perinuclear lysosome compartment...
  79. pmc An endoplasmic reticulum stress response in Arabidopsis is mediated by proteolytic processing and nuclear relocation of a membrane-associated transcription factor, bZIP28
    Jian Xiang Liu
    Plant Sciences Institute, Iowa State University, Ames, Iowa 50011, USA
    Plant Cell 19:4111-9. 2007
    ..Thus, bZIP28 serves as a sensor/transducer in Arabidopsis to mediate ER stress responses related to UPR...
  80. ncbi Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF
    B Alsina
    Mental Retardation Research Center, Department of Psychiatry and Biobehavioral Sciences, 760 Westwood Plaza, NPI 58-258, University of California Los Angeles, Los Angeles, California 90095, USA
    Nat Neurosci 4:1093-101. 2001
    ..Time-lapse confocal microscopy revealed that although most synapses remain stable, synapses are also formed and eliminated as axons branch ..
  81. pmc In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2
    Benjamin R Arenkiel
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Neuron 54:205-18. 2007
    ....
  82. pmc The secretory membrane system in the Drosophila syncytial blastoderm embryo exists as functionally compartmentalized units around individual nuclei
    David Frescas
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 173:219-30. 2006
    ....
  83. pmc Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media
    Luanne Hall-Stoodley
    Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA 15212, USA
    JAMA 296:202-11. 2006
    ....
  84. ncbi Systemic delivery of liposomal short-chain ceramide limits solid tumor growth in murine models of breast adenocarcinoma
    Thomas C Stover
    Department of Pharmacology, Pennsylvania State College of Medicine, Hershey, Pennsylvania 17033, USA
    Clin Cancer Res 11:3465-74. 2005
    ..Together, these results indicate that bioactive ceramide analogues can be incorporated into pegylated liposomal vehicles for improved solubility, drug delivery, and antineoplastic efficacy...
  85. ncbi Corneal confocal microscopy: a non-invasive surrogate of nerve fibre damage and repair in diabetic patients
    R A Malik
    Department of Medicine, Manchester Royal Infirmary, Oxford Road, Manchester, UK
    Diabetologia 46:683-8. 2003
    ..Corneal confocal microscopy is a reiterative, rapid, non-invasive in vivo clinical examination technique capable of imaging corneal ..
  86. ncbi Sequential-replenishment mechanism of exocytosis in pancreatic acini
    T Nemoto
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
    Nat Cell Biol 3:253-8. 2001
    ..This sequential-replenishment mechanism of exocytosis allows the cell to take advantage of a large supply of fusion-ready granules without needing to transport them to the plasma membrane...
  87. pmc Analysis of the effects of chlorhexidine on oral biofilm vitality and structure based on viability profiling and an indicator of membrane integrity
    C K Hope
    Microbiology Unit, Division of Infection and Immunity, Eastman Dental Institute for Oral Health Care Sciences, University College London, London, United Kingdom
    Antimicrob Agents Chemother 48:1461-8. 2004
    ....
  88. ncbi Mucosal biofilm formation on middle-ear mucosa in the chinchilla model of otitis media
    Garth D Ehrlich
    Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA 15212, USA
    JAMA 287:1710-5. 2002
    ....
  89. ncbi HIV-1 Gag-RNA interaction occurs at a perinuclear/centrosomal site; analysis by confocal microscopy and FRET
    Emma Poole
    Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, CB2 2QQ, UK
    Traffic 6:741-55. 2005
    ..HIV-1 Psi thus acts as a subcellular localisation signal as well as a high-affinity-binding site for Gag...
  90. pmc Depletion of membrane cholesterol eliminates the Ca2+-activated component of outward potassium current and decreases membrane capacitance in rat uterine myocytes
    A Shmygol
    Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Coventry CV2 2DX, UK
    J Physiol 581:445-56. 2007
    ....
  91. ncbi The parasite invasion marker SRPN6 reduces sporozoite numbers in salivary glands of Anopheles gambiae
    Sofia B Pinto
    Imperial College London, Faculty of Natural Sciences, Division of Cell and Molecular Biology, Sir Alexander Fleming Building, South Kensington Campus, London, SW7 2AZ, UK
    Cell Microbiol 10:891-8. 2008
    ..Despite several differences between the passage of Plasmodium through the midgut and the salivary glands, this study identifies a striking overlap in the molecular responses of these two epithelia to parasite invasion...
  92. pmc Large currents generate cardiac Ca2+ sparks
    L T Izu
    Department of Medicine, University of Maryland, Baltimore, Baltimore, Maryland 21201, USA
    Biophys J 80:88-102. 2001
    ....
  93. pmc Local recovery of Ca2+ release in rat ventricular myocytes
    Eric A Sobie
    Medical Biotechnology Center, 725 W Lombard Street, Baltimore, MD 21201, USA
    J Physiol 565:441-7. 2005
    ....
  94. ncbi Influence of fibrin network conformation and fibrin fiber diameter on fibrinolysis speed: dynamic and structural approaches by confocal microscopy
    J P Collet
    Department of Cardiology, Hemostasis Laboratory, Pitie Salpetriere Hospital, Hotel Dieu Hospital, Paris, France
    Arterioscler Thromb Vasc Biol 20:1354-61. 2000
    ..cross-linked plasma fibrin was labeled with colloidal gold particles, and fibrinolysis was followed by confocal microscopy. Morphological changes were characterized at fibrin network and fiber levels...
  95. doi Role of MMP9 on invadopodia formation in cells from adenoid cystic carcinoma. Study by laser scanning confocal microscopy
    Camila F Nascimento
    Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil
    Microsc Res Tech 73:99-108. 2010
    ..Cells were also double stained to actin and MT1-MMP. Samples were studied by laser scanning confocal microscopy. In all time points CAC2 cells showed actin, cortactin, and MT1-MMP colocalized with digestion spots in ..
  96. pmc Cellular and subcellular localization of phototropin 1
    Koji Sakamoto
    Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, CA 94305, USA
    Plant Cell 14:1723-35. 2002
    ..Possible relationships between observed phot1 distribution and the various physiological responses activated by blue light are discussed...
  97. pmc Activity-dependent nuclear translocation and intranuclear distribution of NFATc in adult skeletal muscle fibers
    Y Liu
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Cell Biol 155:27-39. 2001
    ..1-s trains at 50 Hz every 50 s. The stimulation pattern-dependent nuclear translocation of NFATc demonstrated here could thus contribute to fast-twitch to slow-twitch fiber type transformation...
  98. pmc Effects of stretch-activated channel blockers on [Ca2+]i and muscle damage in the mdx mouse
    Ella W Yeung
    School of Medical Sciences and Institute for Biomedical Research, University of Sydney F13, NSW 2006, Australia
    J Physiol 562:367-80. 2005
    ..eccentric) contractions while measuring intracellular calcium concentration ([Ca(2+)](i)) with fluo-3 and confocal microscopy. Following the stretched contractions there was a slow rise in resting [Ca(2+)](i) and after 30 min both ..
  99. ncbi Noninvasive imaging of 5-HT3 receptor trafficking in live cells: from biosynthesis to endocytosis
    Erwin Ilegems
    Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology, CH 1015 Lausanne, Switzerland
    J Biol Chem 279:53346-52. 2004
    ..5-HT(3) receptor (5-HT(3)R) were resolved temporally and spatially in live cells by multicolor fluorescence confocal microscopy. The insertion of the enhanced cyan fluorescent protein into the large intracellular loop delivered a ..
  100. ncbi Neurokinin 1 receptor expression by neurons in laminae I, III and IV of the rat spinal dorsal horn that project to the brainstem
    A J Todd
    Spinal Cord Group, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ UK
    Eur J Neurosci 12:689-700. 2000
    ..These results demonstrate that dorsal horn neurons which express the neurokinin 1 receptor contribute to several ascending pathways that are thought to be important in pain mechanisms...
  101. pmc Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis
    Mariusz Karbowski
    Biochemistry Section, SNB, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 159:931-8. 2002
    ..Surprisingly, Drp1 and Mfn2, but not other proteins implicated in the regulation of mitochondrial morphology, colocalize with Bax in these foci. We suggest that Bax participates in apoptotic fragmentation of mitochondria...

Research Grants69

  1. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  2. CRF-Oxytocin Interaction in the Regulation of Stress and Affect
    Joanna Dabrowska; Fiscal Year: 2013
    ..be used to directly address these questions: SA1) adenoviral-based neuronal tracing studies combined with confocal microscopy, SA2) in vitro whole-cell patch-clamp electrophysiological recordings from OT neurons in the PVN, SA3) in ..
  3. Regulation of mural cells during pulmonary capillary formation
    Stephen E McGowan; Fiscal Year: 2013
    ..Laser scanning confocal microscopy (LSCM) and stereology will be used to analyze ingress of LF and pericytes and their association with ..
  4. Inhibition of human neutrophil function by Francisella tularensis
    Lee Ann H Allen; Fiscal Year: 2012
    ..utilize chemiluminescence fluorescence and other biochemical assays to quantify reactive oxygen species;confocal microscopy to localize NADPH oxidase components;immunofluorescence microscopy and immuno-electron microscopy to assess ..
  5. Chronic nicotine and synaptic transmission in brainstem respiratory neurons
    RALPH FRANK FREGOSI; Fiscal Year: 2013
    ..This hypothesis will be tested by filling motoneurons with dyes, and using 3-dimensional confocal microscopy to reconstruct the motoneuron cell body and dendritic tree, followed by detailed measures of somatic and ..
  6. MOLECULAR BASIS OF REOVIRUS PATHOGENESIS
    Terence S Dermody; Fiscal Year: 2013
    ..of JAM-A and JAM-A-associated proteins following reovirus infection of polarized endothelial cells using confocal microscopy and subcellular fractionation...
  7. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
    ..to examine Rac1 function in the mouse embryonic submandibular salivary gland and live time-lapse or fixed confocal microscopy to image the dynamics of branching morphogenesis and apico-basal polarity...
  8. Role for Na,K-ATPase in Nucleoplasmic Calcium Homeostasis
    Craig Gatto; Fiscal Year: 2010
    ..We will combine strategies from molecular biology, cell physiology, biochemistry, electrophysiology and confocal microscopy to accomplish our scientific goals...
  9. Prolonged Inhibition of Pathologic Neovascularization by Catalytic Antioxidants
    JAMES FRANCIS MCGINNIS; Fiscal Year: 2013
    ..on specific genes involved in oxidative stress, inflammation and neovascularization will be analyzed using confocal microscopy, Western blots and PCR arrays...
  10. Novel Peptide MPO Inhibitors for Treating Atherosclerosis
    Kirkwood A Pritchard; Fiscal Year: 2012
    ..Atherosclerotic lesion formation will be determined histologically using immunofluorescence and confocal microscopy. Successful completion of the proposed studies will result in the development of a new class of non-toxic ..
  11. Tissue-specific delivery of probes by control of membrane trafficking of endoprot
    Blake Peterson; Fiscal Year: 2010
    ..elegans will be quantified based on red, green, and FRET fluorescence images obtained from confocal microscopy. This information, combined with other pharmacokinetic parameters such as Vd, half- life in vivo, and ..
  12. Regulation of the Vibrio cholerae intestinal exit plan
    ANISIA J SILVA BENITEZ; Fiscal Year: 2010
    ..a variety of approaches such as bacterial genetics, chromatin immunoprecipitation (ChIP), ChiP-sequencing, confocal microscopy and animal models. In Aim 1, we will determine the regulatory interactions that trigger V...
  13. Mechanism-Based Discovery of Protectants Against Iron-Dependant Oxidative Injury
    JUSTIN M SCHWARTZ; Fiscal Year: 2011
    ..Through use of chelators and confocal microscopy of iron- and calcium-indicating fluorophores, the specific roles of iron and calcium uptake to ..
  14. PATHOPHYSIOLOGY OF THE ENDOTHELIUM
    Francis W Luscinskas; Fiscal Year: 2013
    ..histopathology of mouse tissues;Core C (Physiological and Molecular Imaging) will support intravital and confocal microscopy studies as well as in vitro leukocyte-endothelial adhesion studies under defined flow conditions...
  15. Nuclear-Cytoplasmic Shuttling of Foxc2 via 14-3-3 Determines Proximal Tubule Cell
    DIANA LYNNE GOLDEN; Fiscal Year: 2012
    ..We will use [unreadable]-actinin and paxillin staining (focal adhesions) viewed and quantified by confocal microscopy. Cell migration will be monitored by wounding/sheet assays...
  16. Characterizing TLR Function of Human Dendritic Cells in the Elderly
    Alexander Panda; Fiscal Year: 2012
    ..The PI will employ cell biology techniques including pulse-chase experiments, confocal microscopy and fluorescence resonance energy transfer to evaluate age-associated changes in the kinetics of TLR1 ..
  17. Phosphotyrosine signaling pathways controlling tracheal tube geometry
    KAI G ZINN; Fiscal Year: 2013
    ..Because this is a time-consuming screen, requiring quantitative analysis of individual embryos using confocal microscopy, we will reduce the numbers of lines that need to be screened by using a 'phenotypic screening kit&..
  18. PET Imaging of Myocardial Oxidative Stress in T2DM
    KOORESH ISAAC SHOGHI; Fiscal Year: 2013
    ..induced ROS and a physiological model based on results from Specific Aim 2-by correlation to optical and confocal microscopy measurements...
  19. RSK-2 regulates integrin-mediated adhesion and migration
    JOE WILLIAM RAMOS; Fiscal Year: 2013
    ..These aims will be pursued in tissue culture cells using flow cytometry, confocal microscopy, immunofluorescence, molecular biology, and biochemistry...
  20. Mechanisms of Angiogenesis in Retinopathy of Prematurity
    Mary Elizabeth Hartnett; Fiscal Year: 2009
    ..Methods include: confocal microscopy of retinal flat mounts to visualize endothelial cells at the junction of vascular and avascular retina, to ..
  21. Neuropeptide regulation of neurohypophyseal function
    Francis W Flynn; Fiscal Year: 2012
    ..by VP magnocellular neurons were shown to be translocated from the cell membrane to the cell nucleus by confocal microscopy, immuno-electron microscopy and Western blot. Nuclear NK3R was not detected under basal conditions...
  22. Local Calcium Signaling in Pulmonary Arteries
    JAMES SK SHAM; Fiscal Year: 2012
    ..we will apply a combination of state-of-the-art techniques including whole-cell patch clamp, laser-scanning confocal microscopy, and UV-pulse laser flash photolysis, siRNA, and CD38 knockout mice to determine (1) the Ca2+ signals and ..
  23. Dual-Receptor Targeted Nanoparticles for Photodynamic Therapy of Brain Cancer
    Ann Marie Broome; Fiscal Year: 2013
    ..By improving the selectivity of tumor targeting, we can potentially sustain local delivery of PDT drugs, thus dramatically enhancing the success of brain cancer therapy. ..
  24. Dopamine's Role in the Rapid Estradiol Enhancement of Excitatory Synapses
    CAROLINE COFER SMITH HAMILTON; Fiscal Year: 2013
    ..While I have gained invaluable skills using electrophysiology, immunohistochemistry, confocal microscopy, and stereotactic surgery to address key questions, I have little experience using molecular and ..
  25. Mechanisms of Angiogenesis of Retinopathy of Prematurity
    MARY ELIZABETH RUTH HARTNETT; Fiscal Year: 2013
    ..Methods include: confocal microscopy of retinal flat mounts to visualize endothelial cells at the junction of vascular and avascular retina to ..
  26. Heat Shock Protein Synthesis Inhibitor Treatment of Hepatitis C Viral Infection
    SAMUEL WHEELER FRENCH; Fiscal Year: 2013
    ..We confirmed an NS5A/HSP interaction by confocal microscopy and coimmunoprecipitation...
  27. Brain Delivery of Nanoformulated Superoxide Dismutase
    Erin G Rosenbaugh; Fiscal Year: 2013
    ..using fluorescently-labeled SOD-nano, inhibitors of clathrin and/or caveolae- mediated endocytosis, and confocal microscopy. In Specific Aim 2, immunohistochemical analysis of brain and other tissues and whole animal in vivo ..
  28. Insights from Comparative Genomics for the Treatment of Pneumococcal Infections
    Natalia Luisa Hiller; Fiscal Year: 2011
    ..PUBLIC HEALTH RELEVANCE: The long-term goal of this proposal is the diagnosis and treatment of bacterial-induced ear infections. These infections are the most common illnesses in infants and young children. ..
  29. Imbalances in Calcium Homeostasis: Mechanism Behind Ethanol-Related Dendritic Deg
    Cynthia A Dlugos; Fiscal Year: 2010
    ..Levels of the SERCA 2b ATPase pump and calreticulin will be assessed with confocal microscopy, and fluorescence intensity correlation after treatment durations of 10, 20 or 40 weeks in proximal and ..
  30. Neuropeptide modulation of biogenic amine function and aggression in a crustacean
    Maria A Sosa; Fiscal Year: 2013
    ..and quantitation before and after injections of agents of interest, CNS dissection, immunohistochemistry, confocal microscopy, hemolymph sampling and quantitative mass spectrometry...
  31. Tumor Cell Arrest and Adhesion in the Microcirculation
    Bingmei M Fu; Fiscal Year: 2013
    ..Specific aims are: 1) use quantitative fluorescence video and confocal microscopy to determine the adhesion rates of normal, non-malignant (MCF-10A), and malignant (AU-565) breast ..
  32. Insights from Comparative Genomics for the Treatment of Pneumococcal Infections
    Natalia Luisa Hiller; Fiscal Year: 2013
    ..abstract_text> ..
  33. Iron, NO, and Lipid Peroxide in Photodynamic Therapy
    ALBERT GIROTTI; Fiscal Year: 2013
    ..evaluation;use of NOS inhibitors, NO scavengers, and chemical NO donors;RNA interference;immunoblotting;confocal microscopy;and PDT of human tumors implanted in immunosuppressed mice;in addition to ALA-PDT, classical Photofrin-PDT ..
  34. Biofilm Infections in Postsurgical, Trauma, and Critically Ill Patients
    Carol L Wells; Fiscal Year: 2013
    ..Experimental methods include standard microbiological methods, confocal microscopy and high resolution electron microscopy, cell sorting, fluorescence in situ hybridization, and biochemical ..
  35. Aligned Tendon or Ligament Derived Matrix Nanoscaffolds for Rotator Cuff Repair
    Dianne Little; Fiscal Year: 2013
    ..expansion of current skills in mechanical testing and stress-strain analysis, biomaterial development, confocal microscopy, image and data post-processing and fast Fourier transform analysis, skills essential to the success of the ..
  36. Localization and characterization of the Clostridium difficile biofilm
    Adam Driks; Fiscal Year: 2013
    ..Using laser-scanning confocal microscopy, we found that polysaccharides and nucleic acids are present in the matrix...
  37. MOLECULAR BIOLOGY OF HEMORRHAGIC SHOCK
    Timothy R Billiar; Fiscal Year: 2013
    ..C) will provide extraordinary expertise in state-of-the-art tissue imaging, including immunohistochemistry, confocal microscopy, electron microscopy, quantitative morphology, and in situ hybridization...
  38. Suppression of pathogenic autoantibodies in lupus by inhibition of AID
    John D Mountz; Fiscal Year: 2012
    ..GC formation will be analyzed by confocal microscopy and FACS analysis...
  39. Biomarker Reference Laboratory, EDRN
    William E Grizzle; Fiscal Year: 2010
    ..tissue cylinders), computerized cytomorphometric analysis, immunohistochemistry (bright field fluorescent, confocal microscopy), in situ hybridization, single and multiplex real time quantitative PCR, other PCR techniques, ..
  40. Activity Dependent Signaling in Adult Skeletal Muscle Fiber Plasticity
    Martin F Schneider; Fiscal Year: 2012
    ..transcription factors and other transcriptional modulators can be localized and quantitatively imaged by confocal microscopy in individual living, fully differentiated adult mouse flexor digitorum brevis (FDB) skeletal muscle fibers ..
  41. Immune Regulation of Viral Recrudescence
    Cornelia Bergmann; Fiscal Year: 2012
    ..In situ hybridization combined with confocal microscopy in Aim 1 will determine the spatial relationship between chemokines, viral antigen, ASC localization and ..
  42. Identifying Key Structural Elements in the Activation of the Arp2/3 Complex
    Patricia A Khuu; Fiscal Year: 2013
    ..Phenotype analysis of mutants will be visualized with GFP-tagged markers using confocal microscopy and total internal reflection microscopy (TIRF)...
  43. Regulation of Fibronectin Matrix Assembly by uPAR
    PAULA J MC KEOWN-LONGO; Fiscal Year: 2012
    ..and degradation in response to uPAR or EGFR ligands will be analyzed by Western blot, FLOW cytometry and confocal microscopy. The role of EGFR kinase activity and phosphorylated residues in the regulation of complex formation ..
  44. Role of surface proteins in sand fly colonization by Bartonella bacilliformis
    Michael F Minnick; Fiscal Year: 2013
    ..The second goal is to follow the anatomical locations of the GFP+ Bb strain in the sand fly over time, by confocal microscopy. In Aim 2, we will generate hbpC, ialB and flaA mutants in low-passage Bb strains...
  45. Thiol Isomerases During Thrombus Formation
    Barbara C Furie; Fiscal Year: 2013
    ..ERp72, ERp46, ERdj5, TMX and TMX3 will be studied in a live mouse by intravital multichannel widefield and confocal microscopy. The effect on inhibition of each thiol isomerase using antibodies specific for thiol isomerases on ..
  46. Neuronal mRNA PET Imaging
    Eric Wickstrom; Fiscal Year: 2010
    ..We will also prepare fluorescent probes for confocal microscopy studies. For both gene targets, we will also synthesize and evaluate PNA and peptide mismatch controls...
  47. Inflammation and Repair in Cardiac Ischemia-Reperfusion
    Lewis C Becker; Fiscal Year: 2011
    ..The Cores will provide histology, immunohistology, fluorescent confocal microscopy, and murine models of (1) myocardial ischemia-reperfusion with measurement of infarct size and assessment ..
  48. Contribution of the Streptococcus mutans cid and lrg Murein Hydrolase Regulator H
    KELLY CHRISTINE RICE; Fiscal Year: 2010
    ..The effect of these mutations on cell death and lysis will be measured by a variety of phenotypic assays. Confocal microscopy will be used to assess their effect on biofilm adherence and development in flow cell and static models, as ..
  49. Mechanism and functional role of AKAP79/150 in M current control and excitability
    Mark S Shapiro; Fiscal Year: 2010
    ..used include fluorescence resonance energy transfer (FRET), total internal reflection fluorescence (TIRF), confocal microscopy, patch-clamp electrophysiology, carbon-fiber amperometry and video imaging...
  50. ClC-3 Chloride Ion Channels in Vascular Smooth Muscle
    Fred Lamb; Fiscal Year: 2009
    ..Aim #2 will use fluorescent probes and confocal microscopy to define the contribution of Nox1 and ClC-3 to controlling endosomal pH, Cl- concentration and voltage ..
  51. A Targeted Preemptive Approach to Addressing Mitochondrial Toxicity of Nucleoside
    John K Buolamwini; Fiscal Year: 2013
    ..integrating synthetic medicinal chemistry, biochemical and molecular biological assays, laser scanning confocal microscopy, chromatography and tandem mass spectrometry will be employed...
  52. Pharmacology of Antiretroviral Nanoparticle Micelles
    Christopher J Destache; Fiscal Year: 2010
    ..Liquid chromatography, scanning electron and confocal microscopy, and functional studies in macrophages as a drug delivery system will be gathered...
  53. Regulation of TSLP receptor expression and function in eczema in mice and man
    Steven F Ziegler; Fiscal Year: 2013
    ..will be analyzed on discrete DC types in lesional skin and at atopy patch test sites by flow cytometry and confocal microscopy techniques...
  54. Autophagy and human islet amyloid polypeptide in animal models of type 2 diabetes
    Jacqueline Rivera; Fiscal Year: 2013
    ..to autophagosomes or lysosomes to visualize transport and fusion abnormalities in real time experiments by confocal microscopy (live cell imaging)...
  55. Protein Handling By Renal Proximal Tubule Epithelial Cells
    Nicholas Ferrell; Fiscal Year: 2013
    ..trafficking of albumin will be characterized under flow and static conditions using spinning disk confocal microscopy and size exclusion chromatography to determine if flow induced cytoskeletal remodeling alters the capacity ..
  56. A translational model of neuro-immune therapy for PTSD in veterans of the OEF/OIF
    Leonardo H Tonelli; Fiscal Year: 2013
    ..Immunohistochemistry and confocal microscopy will be employed to analyze the presence of T cells in the brain and the expression of neurotrophic factors...
  57. Laser Spinning Disc Confocal System for live-cell and live-organism microscopy
    Martha C Soto; Fiscal Year: 2012
    ..PUBLIC HEALTH RELEVANCE: The addition of a laser spinning disc confocal microscopy system for the researchers at two adjacent universities on a shared campus, Rutgers University and Robert ..
  58. Mechanisms of Cell Death after Photodynamic Therapy
    Anna Liisa Nieminen; Fiscal Year: 2010
    ..cytosolic, mitochondrial and ER Ca2+ after PDT using compartmentally localized fluorescence indicators and Confocal microscopy. Using specific interventions, we will determine whether depletion of ER Ca2+ stores, inhibition of ..
  59. MOLECULAR PROPERTIES OF IONIC PERMEABILITY IN NERVE
    Bertil Hille; Fiscal Year: 2013
    ..biophysics, electrophysiology, fluorescence resonance energy transfer, photometry, molecular biology, and confocal microscopy. The focus will be on the phosphatidyl inositide phospholipids as regulators and signals for channel ..
  60. NHERF-1 and dopamine-mediated regulation of renal phosphate transport.
    Edward J Weinman; Fiscal Year: 2013
    ..we will first determine if NHERF-1 binds to the dopamine D1-like receptor using immunoprecipitation and confocal microscopy. Using 32P labeled cells, we will then determine if occupancy of the dopamine D1-like receptor results in ..
  61. OPTICAL BIOPSY USING OPTICAL COHERENCE TOMOGRAPHY
    James G Fujimoto; Fiscal Year: 2012
    ..Optical coherence microscopy (OCM) combines OCT with confocal microscopy to achieve cellular level resolution with an extended imaging depth...
  62. Ca2+ and ROS Crosstalk Signaling in Cardiac Mitochondria
    Shey Shing Sheu; Fiscal Year: 2013
    ..We will use a multidisciplinary approach encompassing techniques of cell biology (e.g. confocal microscopy), molecular biology (e.g. gene transfer), biochemistry (e.g. western blots), and transgenic mouse models (e...
  63. NIH Director's Pioneer Award
    Karl Deisseroth; Fiscal Year: 2009
    ..abstract_text> ..
  64. ADIPOCYTE PROTEIN SECRETION AND INSULIN ACTION
    Harvey Lodish; Fiscal Year: 2007
    ..In specific aim 2, he will use confocal microscopy and subcellular fractionation to study the intracellular location and protein composition of vesicles ..
  65. Novel Use of Confocal Microscopy on Cultured Porcine Corneas for Pre-Clinical Tes
    Michelle Piehl; Fiscal Year: 2011
    ..to potentially predict human eye sting due to measurement of individual cell death per tissue volume by confocal microscopy. The PorFocal uses waste porcine corneas from the meat industry to assay individual corneal cell death with ..
  66. Pathogenesis of Lyme Borreliosis in the Rhesus Monkey
    MARIO PHILIPP; Fiscal Year: 2007
    ..secretion (IL-6, TNF-alpha, and IL-1 beta) as well as expression of iNOS and COX-2 will be assessed by confocal microscopy using a novel procedure whereby cytokines and other inflammatory mediators are stained intracellularly with ..
  67. Virulence Factor Trafficking in Cryptococcus
    Peter Williamson; Fiscal Year: 2005
    ..antibody, 2) localization to the periphery of a green-fluorescent protein-tagged recombinant laccase by confocal microscopy, 3) localization of enzyme activity and laccase immunoreactive protein to cell wall preparations of C...
  68. In Vivo Imaging of Leukocyte-Endothelial Dynamics
    William Mathers; Fiscal Year: 2005
    ..Recent advances in confocal microscopy have allowed a European group to quantitate leukocyte endothelial rolling and sticking in the ..
  69. Redox Biology in COPD
    Yvonne Janssen Heininger; Fiscal Year: 2009
    ..Completion of this grant proposal may also offer new insights into the mechanisms whereby oxidants regulate the severity of inflammation. ..