microinjections

Summary

Summary: The injection of very small amounts of fluid, often with the aid of a microscope and microsyringes.

Top Publications

  1. ncbi Laser-engineered dissolving microneedle arrays for transdermal macromolecular drug delivery
    Katarzyna Migalska
    School of Pharmacy, Queen s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK
    Pharm Res 28:1919-30. 2011
  2. ncbi PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development
    Christopher M Saunders
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Development 129:3533-44. 2002
  3. pmc Design, optimization and characterisation of polymeric microneedle arrays prepared by a novel laser-based micromoulding technique
    Ryan F Donnelly
    School of Pharmacy, Queen s University Belfast Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, UK
    Pharm Res 28:41-57. 2011
  4. pmc Inhibition of cardiac sympathetic afferent reflex and sympathetic activity by baroreceptor and vagal afferent inputs in chronic heart failure
    Xian bing Gan
    Department of Physiology, Nanjing Medical University, Nanjing, China
    PLoS ONE 6:e25784. 2011
  5. ncbi Microneedles for transdermal drug delivery
    Mark R Prausnitz
    School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA 30332 0100, USA
    Adv Drug Deliv Rev 56:581-7. 2004
  6. ncbi Microinjections of acetaldehyde or salsolinol into the posterior ventral tegmental area increase dopamine release in the nucleus accumbens shell
    Gerald A Deehan
    Department of Psychiatry, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Alcohol Clin Exp Res 37:722-9. 2013
  7. ncbi Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells
    M A Sells
    Fox Chase Cancer Centre, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA
    Curr Biol 7:202-10. 1997
  8. ncbi Paraventricular nucleus is involved in the central pathway of cardiac sympathetic afferent reflex in rats
    Ming Kui Zhong
    Department of Physiology, Nanjing Medical University, Nanjing 210029, China
    Exp Physiol 93:746-53. 2008
  9. pmc Processing difficulties and instability of carbohydrate microneedle arrays
    Ryan F Donnelly
    Medical Biology Centre, School of Pharmacy, Queen s University Belfast, Belfast, UK
    Drug Dev Ind Pharm 35:1242-54. 2009
  10. ncbi Hydrogel swelling as a trigger to release biodegradable polymer microneedles in skin
    Minyoung Kim
    College of BioNano Technology and Gachon BioNano Research Institute, Gachon University, Seongnam si, Gyeonggi do 461 701, Republic of Korea
    Biomaterials 33:668-78. 2012

Detail Information

Publications299 found, 100 shown here

  1. ncbi Laser-engineered dissolving microneedle arrays for transdermal macromolecular drug delivery
    Katarzyna Migalska
    School of Pharmacy, Queen s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK
    Pharm Res 28:1919-30. 2011
    ..To assess the feasibility of transdermal macromolecule delivery using novel laser-engineered dissolving microneedles (MNs) prepared from aqueous blends of 20% w/w poly(methylvinylether maleic anhydride) (PMVE/MA) in vitro and in vivo...
  2. ncbi PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development
    Christopher M Saunders
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Development 129:3533-44. 2002
    ..Our results are consistent with sperm PLC zeta as the molecular trigger for development of a fertilised egg into an embryo...
  3. pmc Design, optimization and characterisation of polymeric microneedle arrays prepared by a novel laser-based micromoulding technique
    Ryan F Donnelly
    School of Pharmacy, Queen s University Belfast Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, UK
    Pharm Res 28:41-57. 2011
    ..The aim of this study was to optimise polymeric composition and assess the performance of microneedle devices that possess different geometries...
  4. pmc Inhibition of cardiac sympathetic afferent reflex and sympathetic activity by baroreceptor and vagal afferent inputs in chronic heart failure
    Xian bing Gan
    Department of Physiology, Nanjing Medical University, Nanjing, China
    PLoS ONE 6:e25784. 2011
    ..This study was designed to determine whether it is true in intact (INT) rats with CHF and to determine the effects of cardiac and baroreceptor afferents on the CSAR and sympathetic activity in CHF...
  5. ncbi Microneedles for transdermal drug delivery
    Mark R Prausnitz
    School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA 30332 0100, USA
    Adv Drug Deliv Rev 56:581-7. 2004
    ..Together, these results suggest that microneedles represent a promising technology to deliver therapeutic compounds into the skin for a range of possible applications...
  6. ncbi Microinjections of acetaldehyde or salsolinol into the posterior ventral tegmental area increase dopamine release in the nucleus accumbens shell
    Gerald A Deehan
    Department of Psychiatry, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Alcohol Clin Exp Res 37:722-9. 2013
    ..The current study was undertaken to examine the dose-response effects of microinjections of ACD or SAL into the pVTA on DA efflux in the nucleus accumbens shell (AcbSh).
  7. ncbi Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells
    M A Sells
    Fox Chase Cancer Centre, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA
    Curr Biol 7:202-10. 1997
    ..This kinase is therefore a candidate effector for the changes in cell shape induced by growth factors...
  8. ncbi Paraventricular nucleus is involved in the central pathway of cardiac sympathetic afferent reflex in rats
    Ming Kui Zhong
    Department of Physiology, Nanjing Medical University, Nanjing 210029, China
    Exp Physiol 93:746-53. 2008
    ..These results indicate that inhibition or lesion of the PVN abolishes the CSAR, but excitation of the neurons in the PVN enhances the CSAR, suggesting that the PVN is an important component of the central neurocircuitry of the CSAR...
  9. pmc Processing difficulties and instability of carbohydrate microneedle arrays
    Ryan F Donnelly
    Medical Biology Centre, School of Pharmacy, Queen s University Belfast, Belfast, UK
    Drug Dev Ind Pharm 35:1242-54. 2009
    ....
  10. ncbi Hydrogel swelling as a trigger to release biodegradable polymer microneedles in skin
    Minyoung Kim
    College of BioNano Technology and Gachon BioNano Research Institute, Gachon University, Seongnam si, Gyeonggi do 461 701, Republic of Korea
    Biomaterials 33:668-78. 2012
    ..Model drugs encapsulated in biodegradable polymer microneedles and hydrogel microparticles were successfully delivered by releasing microneedles into the skin...
  11. ncbi Medial prefrontal cortex determines how stressor controllability affects behavior and dorsal raphe nucleus
    J Amat
    Department of Psychology, Campus Box 345, University of Colorado, Boulder, Colorado 80903 0345, USA
    Nat Neurosci 8:365-71. 2005
    ..This suggests a new function for the mPFCv and implies that the presence of control inhibits stress-induced neural activity in brainstem nuclei, in contrast to the prevalent view that such activity is induced by a lack of control...
  12. pmc Microneedle-mediated transdermal bacteriophage delivery
    Elizabeth Ryan
    School of Pharmacy, Queen s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, UK
    Eur J Pharm Sci 47:297-304. 2012
    ..Accordingly, bacteriophage-based therapeutics may now have an alternative route for systemic delivery. Once fully-investigated, this could lead to more widespread investigation of these interesting therapeutic viruses...
  13. ncbi Targeted integration in rat and mouse embryos with zinc-finger nucleases
    Xiaoxia Cui
    Sigma Advanced Genetic Engineering Labs, Sigma Aldrich Biotechnology, St Louis, Missouri, USA
    Nat Biotechnol 29:64-7. 2011
    ..The same strategy can potentially be applied to many other species for which genetic engineering tools are needed...
  14. ncbi Actomyosin transports microtubules and microtubules control actomyosin recruitment during Xenopus oocyte wound healing
    Craig A Mandato
    Department of Zoology, University of Wisconsin Madison, Madison, WI 53706, USA
    Curr Biol 13:1096-105. 2003
    ..We have analyzed the role of microtubules in generation of a contractile array comprised of F-actin and myosin-2 that forms around wounds made in Xenopus oocytes...
  15. ncbi Double-stranded RNA induces specific developmental defects in zebrafish embryos
    A Wargelius
    Department of Molecular Biology, University of Bergen, HIB, Bergen, N 5020, Norway
    Biochem Biophys Res Commun 263:156-61. 1999
    ..These findings suggest that dsRNA-mediated interference will become an important tool for analysing the functional roles of genes in zebrafish and other vertebrates...
  16. ncbi Polymer microneedles for controlled-release drug delivery
    Jung Hwan Park
    Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Pharm Res 23:1008-19. 2006
    ....
  17. pmc Microfabricated needles for transdermal delivery of macromolecules and nanoparticles: fabrication methods and transport studies
    Devin V McAllister
    School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta 30332, USA
    Proc Natl Acad Sci U S A 100:13755-60. 2003
    ..Hollow microneedles permitted flow of microliter quantities into skin in vivo, including microinjection of insulin to reduce blood glucose levels in diabetic rats...
  18. ncbi Dynamic organization of DNA replication in one-cell mouse embryos: relationship to transcriptional activation
    C Bouniol-Baly
    Laboratoire Associé Institut National de la Santé et de la Recherche Médicale INSERM, Institut National de la Recherche Agronomique INRA, Paris, France
    Exp Cell Res 236:201-11. 1997
    ..Moreover, studies on aphidicolin-treated embryos demonstrated that inhibition of DNA replication does not hinder transcriptional activation at the 1-cell stage...
  19. ncbi Angiotensin-mediated increase in renal sympathetic nerve discharge within the PVN: role of nitric oxide
    Yi Fan Li
    Division of Basic Biomedical Science, College of Medicine, University of South Dakota, Vermillion, South Dakota, USA
    Am J Physiol Regul Integr Comp Physiol 290:R1035-43. 2006
    ..This negative-feedback mechanism within the PVN may play an important role in maintaining the overall balance and tone of sympathetic outflow...
  20. ncbi ANG II in the paraventricular nucleus potentiates the cardiac sympathetic afferent reflex in rats with heart failure
    Guo Qing Zhu
    Department of Physiology, Nanjing Medical University, Nanjing 210029, Peoples Republic of China
    J Appl Physiol 97:1746-54. 2004
    ....
  21. ncbi The Ran GTPase mediates chromatin signaling to control cortical polarity during polar body extrusion in mouse oocytes
    Manqi Deng
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Dev Cell 12:301-8. 2007
    ..We hypothesize that a Ran(GTP) gradient serves as a molecular ruler to interpret the asymmetric position of the meiotic chromatin...
  22. pmc Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest
    R A Fratti
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Cell Biol 154:631-44. 2001
    ..tuberculosis. This study also identifies mycobacterial phosphoinositides as products with specialized toxic properties, interfering with discrete trafficking stages in phagosomal maturation...
  23. pmc Generation of a novel Wolbachia infection in Aedes albopictus (Asian tiger mosquito) via embryonic microinjection
    Zhiyong Xi
    Department of Entomology, University of Kentucky, Lexington, KY 40546, USA
    Insect Biochem Mol Biol 35:903-10. 2005
    ..We discuss the results in relation to applied mosquito control strategies and the evolution of Wolbachia infections in Ae. albopictus...
  24. ncbi Pronuclear injection for the production of transgenic mice
    Lars M Ittner
    Alzheimer s and Parkinson s Disease Laboratory, Brain and Mind Research Institute, University of Sydney, 100, Mallett Street, Camperdown, New South Wales 2050, Australia
    Nat Protoc 2:1206-15. 2007
    ..Transgenic expression of a truncated form of the microtubule-associated protein tau (delta tau) is used as an example for the anticipated results...
  25. ncbi fgf17b, a novel member of Fgf family, helps patterning zebrafish embryos
    Ying Cao
    Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China
    Dev Biol 271:130-43. 2004
    ..Like fgf8, activation of fgf17b expression depends on Nodal signaling...
  26. ncbi Intra-accumbens amphetamine increases the conditioned incentive salience of sucrose reward: enhancement of reward "wanting" without enhanced "liking" or response reinforcement
    C L Wyvell
    Department of Psychology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Neurosci 20:8122-30. 2000
    ..We conclude that nucleus accumbens dopamine specifically mediates the ability of reward cues to trigger "wanting" (incentive salience) for their associated rewards, independent of both hedonic impact and response reinforcement...
  27. pmc Knockout rats via embryo microinjection of zinc-finger nucleases
    Aron M Geurts
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 52336, USA
    Science 325:433. 2009
    ..Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models...
  28. ncbi Effects of microinjections of apomorphine and haloperidol into the inferior colliculus on the latent inhibition of the conditioned emotional response
    Liana L Melo
    Laboratorio de Neuropsicofarmacologia, Universidade São Francisco, Braganca Paulista, SP, Brazil
    Exp Neurol 216:16-21. 2009
    ..PE and NPE rats received IC microinjections of physiological saline, the dopaminergic agonist apomorphine (9.0 microg/0...
  29. pmc CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase
    T J Yen
    Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205
    EMBO J 10:1245-54. 1991
    ..This demonstrates that CENP-E function is required for the transition from metaphase to anaphase...
  30. ncbi Molecular mechanisms associated with the antidepressant effects of the class I histone deacetylase inhibitor MS-275 in the rat ventrolateral orbital cortex
    Huali Lin
    Department of Psychiatry, Xi an Mental Health Center, No 15 Yanyin Road, Xi an, Shannxi, China
    Brain Res 1447:119-25. 2012
    ..Our findings suggest the possibility that alterations in gene expression due to chromatin remodeling, including upregulation of CREB and BDNF, may be involved in the antidepressant-like effect of HDACis in the VLO...
  31. pmc Dissolving polymeric microneedle arrays for electrically assisted transdermal drug delivery
    Martin J Garland
    School of Pharmacy, Queen s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast, UK
    J Control Release 159:52-9. 2012
    ..40%) for MN in combination with anodal ITP (p<0.001). As such, drug loaded soluble PMVE/MA MN arrays show promise for the electrically controlled transdermal delivery of biomacromolecules in a simple, one-step approach...
  32. ncbi H1-histamine receptors in the amygdala are involved in emotional memory but do not mediate anxiety-related behaviors in mice submitted to EPM testing
    K R Serafim
    Laboratory of Neuroscience, Physiotherapy Department, Center of Biological Sciences and Health, Federal University of Sao Carlos, Rod Washington Luis, Sao Carlos, Brazil
    Brain Res Bull 89:1-7. 2012
    ..Taken together, these results suggest that the H(1) receptors in the amygdala are not implicated in anxiety-like behaviors but are involved in emotional states induced by the T1/T2 EPM protocol in mice...
  33. ncbi Behavioural and neuronal activation after microinjections of AMPA and NMDA into the perifornical lateral hypothalamus in rats
    Frederick W Li
    Department of Anatomy and Neurobiology, Faculty of Medicine, Dalhousie University, 1459 Oxford Street, Halifax, Nova Scotia, B3H 4R2, Canada
    Behav Brain Res 224:376-86. 2011
    ..The present study examined behavioural effects of activating PeFLH neurons with microinjections of ionotropic glutamate receptor agonists...
  34. ncbi Inactivation of the infralimbic prefrontal cortex reinstates goal-directed responding in overtrained rats
    Etienne Coutureau
    School of Psychology, Cardiff University, PO Box 901, Park Place, Cardiff, CF10 3YG, UK
    Behav Brain Res 146:167-74. 2003
    ..This suggests that the development of habitual responding reflects the active inhibition of goal-directed responses that are mediated by action-outcome associations...
  35. ncbi In vitro characterization of the invasiveness of polymer microneedle against skin
    Young Wook Noh
    College of Pharmacy and Institute of Drug Research and Development, Chungnam National University, Daejeon 305 764, South Korea
    Int J Pharm 397:201-5. 2010
    ..Taken together, polymer MN might be a suitable tool for safe transdermal drug delivery of small molecules...
  36. ncbi Enhancement of extinction memory consolidation: the role of the noradrenergic and GABAergic systems within the basolateral amygdala
    Daniel J Berlau
    Center for the Neurobiology of Learning and Memory, Department of Neurobiology and Behavior, University of California, Irvine, CA 92697 3800, USA
    Neurobiol Learn Mem 86:123-32. 2006
    ..The findings also suggest that the BLA influence on extinction is not mediated by an interaction with the dorsal hippocampus...
  37. ncbi Blockade of endogenous angiotensin-(1-7) in the hypothalamic paraventricular nucleus reduces renal sympathetic tone
    Ana Quênia Gomes da Silva
    Departamento de Fisiologia e Biofisica, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
    Hypertension 46:341-8. 2005
    ..In rats anesthetized with urethane (1.2 to 1.4 g/kg IP), bilateral microinjections of A-779 (0...
  38. ncbi Role of gamma-aminobutyric acid (GABA)A and GABAB receptors in paraventricular nucleus in control of sympathetic vasomotor tone in hypertension
    De Pei Li
    Department of Anesthesiology, Pennsylvania State University, College of Medicine, Hershey, USA
    J Pharmacol Exp Ther 320:615-26. 2007
    ..Collectively, this study provides new evidence that GABA(A) receptor function is attenuated, whereas the function of GABA(B) receptors is enhanced, in the PVN of SHRs...
  39. pmc In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5
    Thomas Ohrt
    Institute for Biophysics, BIOTEC, Dresden University of Technology, Tatzberg 47 51, 01307 Dresden, Germany
    Nucleic Acids Res 34:1369-80. 2006
    ..Exportin 5 therefore represents a key point of intersection between the siRNA and miRNA pathways, and, as such, is of fundamental importance for the design and interpretation of RNA interference experimentation...
  40. ncbi Microneedle technologies for (trans)dermal drug and vaccine delivery
    Koen van der Maaden
    Division of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research LACDR, Leiden University, PO Box 2300 RA, Leiden, The Netherlands
    J Control Release 161:645-55. 2012
    ..Finally, we give our view on research and development that is needed to render microneedle-based (trans)dermal drug delivery technologies clinically useful in the near future...
  41. ncbi The role of dopamine receptors in ventrolateral orbital cortex-evoked antinociception in a rat formalin test model
    Yong Hui Dang
    Department of Forensic Medicine, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi an Jiaotong University School of Medicine, Yanta Road West 76, Xi an, Shaanxi 710061, China
    Eur J Pharmacol 657:97-103. 2011
    ..injection into the rat unilateral hindpaw pad, the effects of dopamine receptor agonist and antagonist microinjections into the VLO on nociceptive behavior were observed...
  42. ncbi Cardiovascular effects of adrenocorticotropin microinjections into the rostral ventrolateral medullary pressor area of the rat
    Tetsuya Kawabe
    Department of Neurological Surgery, MSB H 586, University of Medicine and Dentistry of New Jersey New Jersey Medical School, Newark, 07103, USA
    Brain Res 1102:117-26. 2006
    ..The RVLM was identified by microinjections (100 nl) of L-glutamate (L-Glu). Microinjections (100 nl) of ACTH (0...
  43. pmc Visualization of mRNA localization in Xenopus oocytes
    James A Gagnon
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA
    Methods Mol Biol 714:71-82. 2011
    ..This technique overcomes a significant hurdle of imaging RNA in the frog oocyte while providing a rapid method of visualizing mRNA localization in high resolution...
  44. pmc Quantitative microinjection of oocytes, eggs, and embryos
    Laurinda A Jaffe
    Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    Methods Cell Biol 74:219-42. 2004
  45. ncbi Distinct profiles of alpha7 nAChR positive allosteric modulation revealed by structurally diverse chemotypes
    Jens Halvard Grønlien
    Oslo Research Park, Oslo, Norway
    Mol Pharmacol 72:715-24. 2007
    ..In summary, our studies reveal two distinct alpha7 PAM profiles, which could offer unique opportunities for modulating alpha7 nAChRs in vivo and in the development of novel therapeutics for central nervous system indications...
  46. pmc Structural rearrangements of NR1/NR2A NMDA receptors during allosteric inhibition
    Marc Gielen
    Laboratoire de Neurobiologie, Ecole Normale Superieure, CNRS, 46 rue d Ulm, 75005 Paris, France
    Neuron 57:80-93. 2008
    ....
  47. pmc Mitochondrial release of caspase-2 and -9 during the apoptotic process
    S A Susin
    Centre National de la Recherche Scientifique, UPR 420, F 94801 Villejuif, France
    J Exp Med 189:381-94. 1999
    ....
  48. ncbi Intracerebral microinjections of delta 9-tetrahydrocannabinol: search for the impairment of spatial memory in the eight-arm radial maze in rats
    Nobuaki Egashira
    Department of Physiology and Pharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814 0180, Japan
    Brain Res 952:239-45. 2002
    ..Rats were tested in the eight-arm radial maze after microinjections of delta(9)-THC into one of 14 different brain regions...
  49. ncbi Microinjection of morphine into various amygdaloid nuclei differentially affects nociceptive responsiveness and RVM neuronal activity
    Steve McGaraughty
    Department of Neurological Surgery, Oregon Health Sciences University, Portland, OR 97201, USA
    Pain 96:153-62. 2002
    ..As with basolateral microinjections, ongoing activity of the OFF cells was increased, and although the ongoing firing of ON cells was not ..
  50. ncbi Differential effects on delayed non-matching-to-position in rats of microinjections of muscarinic receptor antagonist scopolamine or NMDA receptor antagonist MK-801 into the dorsal or ventral extent of the hippocampus
    J K Robinson
    Department of Psychology, State University of New York at Stony Brook, 11794 2500, USA
    Brain Res 765:51-60. 1997
    ....
  51. pmc Effects of RAS on the genesis of embryonal rhabdomyosarcoma
    David M Langenau
    Stem Cell Program and Division of Hematology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Genes Dev 21:1382-95. 2007
    ..When coupled with gene expression studies of this cell population, we propose that the zebrafish RMS cancer stem cell shares similar self-renewal programs as those found in activated satellite cells...
  52. ncbi Zinc-finger nucleases: a powerful tool for genetic engineering of animals
    Séverine Rémy
    INSERM, U643, 44093, Nantes, France
    Transgenic Res 19:363-71. 2010
    ..Further refinements of ZFN technology in combination with HR may allow knock-ins in early embryos even in species where ES cells or cloning techniques are available...
  53. ncbi KIN241: a gene involved in cell morphogenesis in Paramecium tetraurelia reveals a novel protein family of cyclophilin-RNA interacting proteins (CRIPs) conserved from fission yeast to man
    A Krzywicka
    , Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette Cedex, France
    Mol Microbiol 42:257-67. 2001
    ..No Kin241-1 protein is present in mutant cells, suggesting that the C-terminal serine-rich region is responsible for protein stability...
  54. ncbi Double-stranded RNA injection produces null phenotypes in zebrafish
    Y X Li
    Developmental Biology Program, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, 30912 2640, USA
    Dev Biol 217:394-405. 2000
    ..1 and resulted in absent or greatly reduced eye and forebrain development, similar to the phenotype seen in mouse mutants. Simultaneous injection of Pax6.1 and Zf-T resulted in embryos lacking notochords, eyes, and brain structures...
  55. ncbi Incentive sensitization by previous amphetamine exposure: increased cue-triggered "wanting" for sucrose reward
    C L Wyvell
    Department of Psychology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Neurosci 21:7831-40. 2001
    ..g., investigatory sniffing) that interfered with goal-directed lever pressing. These results support the incentive-sensitization theory postulate that sensitization causes excessive cue-triggered "wanting" for an associated reward...
  56. ncbi Optical coherence tomography is a valuable tool in the study of the effects of microneedle geometry on skin penetration characteristics and in-skin dissolution
    Ryan F Donnelly
    School of Pharmacy, Medical Biology Centre, Queen s University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK
    J Control Release 147:333-41. 2010
    ..The successful use of OCT in this study could prove to be a key development for polymeric MN research, accelerating their commercial exploitation...
  57. pmc Arrangement of subunits in functional NMDA receptors
    Catherine L Salussolia
    Graduate Program in Neuroscience, Center for Nervous System Disorders, State University of New York at Stony Brook, Stony Brook, New York 11794 5230, USA
    J Neurosci 31:11295-304. 2011
    ..GluN subunits are therefore positioned in a N1/N2/N1/N2 arrangement in functional NMDA receptors...
  58. ncbi Biodegradable polymer microneedles: fabrication, mechanics and transdermal drug delivery
    Jung Hwan Park
    The Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, GA 30332, USA
    J Control Release 104:51-66. 2005
    ..Altogether, these results indicate that biodegradable polymer microneedles can be fabricated with an appropriate geometry and sufficient strength to insert into skin, and thereby dramatically increase transdermal transport of molecules...
  59. ncbi A molecular mechanism for mRNA trafficking in neuronal dendrites
    Jianguo Shan
    Department of Biochemistry and Molecular Biology, University of Queensland, Queensland 4072, Australia
    J Neurosci 23:8859-66. 2003
    ..A2RE-like sequences are found in a subset of dendritically localized mRNAs, which, together with these results, suggests that a molecular mechanism based on this cis-acting sequence may contribute to dendritic RNA localization...
  60. ncbi Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillations
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, School of Medicine, Cardiff University, Cardiff CF14 4XN
    J Biol Chem 280:31011-8. 2005
    ..These data suggest that the exquisite Ca2+-dependent features of PLCzeta regulation are essential for it to generate inositol 1,4,5-trisphosphate and Ca2+ oscillations in intact mouse eggs...
  61. ncbi Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes
    L J Cox
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Reproduction 124:611-23. 2002
    ..These results are consistent with the proposal that sperm PLCzeta is the molecular trigger for egg activation during fertilization and that the role and activity of PLCzeta is highly conserved across mammalian species...
  62. ncbi A cellular mechanism for the antinociceptive effect of a kappa opioid receptor agonist
    M A Ackley
    Department of Anesthesia and Critical Care, University of Chicago, Chicago, IL 60637, USA
    Pain 91:377-88. 2001
    ....
  63. ncbi Expanding the role of HsEg5 within the mitotic and post-mitotic phases of the cell cycle
    C M Whitehead
    Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada
    J Cell Sci 111:2551-61. 1998
    ..and/or determining the stability of specific microtubule arrays that form during cell division and that this role may encompass the ability of HsEg5 to influence the distribution of other protein components associated with cell division..
  64. ncbi Lidocaine and muscimol microinjections in subthalamic nucleus reverse Parkinsonian symptoms
    R Levy
    Department of Physiology, Faculty of Medicine, University of Toronto, Canada
    Brain 124:2105-18. 2001
    ..In this study, microinjections of lidocaine (n = 4) and muscimol, a GABA(A) receptor agonist (n = 2), were performed in the STN of six ..
  65. ncbi Involvement of 5HT1A receptors in the anxiolytic-like effects of cannabidiol injected into the dorsolateral periaqueductal gray of rats
    Alline Cristina Campos
    Department of Pharmacology, School of Medicine of Ribeirao Preto, Campus USP, Av Bandeirantes 3900, Monte Alegre, Ribeirao Preto, SP, Brazil
    Psychopharmacology (Berl) 199:223-30. 2008
    ..In addition, since CBD has been shown to inhibit anandamide metabolism, CB1 receptors could also be involved in the effects of this cannabinoid...
  66. pmc Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina
    Ryan Thummel
    Department of Biological Sciences and the Center for Zebrafish Research, University of Notre Dame, Notre Dame, Indiana 46556, USA
    Dev Neurobiol 68:392-408. 2008
    ....
  67. ncbi Chromatin-mediated cortical granule redistribution is responsible for the formation of the cortical granule-free domain in mouse eggs
    Manqi Deng
    Center for Research on Reproduction and Women s Health and Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Dev Biol 257:166-76. 2003
    ..Results of these experiments suggest that CG redistribution is the dominant factor in formation of the CGFD...
  68. ncbi Lentiviral vectors: are they the future of animal transgenesis?
    Frank Park
    Department of Medicine, Kidney Disease Center, Medical College of Wisconsin, Wauwatosa, Wisconsin 53226, USA
    Physiol Genomics 31:159-73. 2007
    ..other approaches to produce transgenic animals will be compared with regard to efficiency, the ability to promote persistent transgene expression, and the time necessary to generate a sufficient number of animals for phenotyping...
  69. ncbi Histamine enhances inhibitory avoidance memory consolidation through a H2 receptor-dependent mechanism
    Weber C da Silva
    Centro de Memoria, Instituto de Pesquisas Biomédicas, Pontificia Universidade Catolica do Rio Grande do Sul, Porto Alegre, Brazil
    Neurobiol Learn Mem 86:100-6. 2006
    ....
  70. pmc Interaction between cardiac sympathetic afferent reflex and chemoreflex is mediated by the NTS AT1 receptors in heart failure
    Wei Zhong Wang
    Department of Cellular and Integrative Physiology, University of Nebraska Medical Ctr, 985850 Nebraska Medical Ctr, Omaha, NE 68198 5850, USA
    Am J Physiol Heart Circ Physiol 295:H1216-H1226. 2008
    ..These results demonstrate that the enhanced cardiac sympathetic afferent input contributes to an excitatory effect of chemoreflex function in CHF, which is mediated by an NTS-AT1R-dependent mechanism...
  71. pmc Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine
    H S Gill
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA
    Gene Ther 17:811-4. 2010
    ..Thus, microneedles may offer a promising technology for DNA vaccination...
  72. pmc Micro-scale devices for transdermal drug delivery
    Anubhav Arora
    Biomolecular Science and Engineering, University of California, Santa Barbara, CA 93106, USA
    Int J Pharm 364:227-36. 2008
    ..These include liquid jet injectors, powder injectors, microneedles and thermal microablation. The historical perspective, mechanisms of action, important design parameters, applications and challenges are discussed for each method...
  73. ncbi Xvelo1 uses a novel 75-nucleotide signal sequence that drives vegetal localization along the late pathway in Xenopus oocytes
    Maike Claussen
    Institut fur Biochemie und Molekulare Zellbiologie, Georg August Universitat, D 37077 Gottingen, Germany
    Dev Biol 266:270-84. 2004
    ..These observations suggest that the structural features recognized by the protein machinery that drives localization of maternal mRNAs along the late pathway in Xenopus oocytes must be redefined...
  74. pmc Localization of Mad2 to kinetochores depends on microtubule attachment, not tension
    J C Waters
    Biology Department, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
    J Cell Biol 141:1181-91. 1998
    ..We conclude that kinetochore microtubule attachment stops the Mad2 interactions at kinetochores which are important for inhibiting anaphase onset...
  75. pmc Endothelin-1 in paraventricular nucleus modulates cardiac sympathetic afferent reflex and sympathetic activity in rats
    Ai Dong Chen
    Department of Physiology, Nanjing Medical University, Nanjing, China
    PLoS ONE 7:e40748. 2012
    ..The present study is designed to determine whether endothelin-1 (ET-1) in the PVN modulates the CSAR and sympathetic activity, and whether superoxide anions are involved in modulating the effects of ET-1 in the PVN in rats...
  76. ncbi Microinjection of cytoplasm or mitochondria derived from somatic cells affects parthenogenetic development of murine oocytes
    Kumiko Takeda
    Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science, National Agricultural and Bio Oriented Research Organization, Tsukuba, Ibaraki, 305 0901, Japan
    Biol Reprod 72:1397-404. 2005
    ..These results have further implications for in vitro fertilization protocols employing ooplasmic transfer where primary oocyte failure is not confirmed...
  77. ncbi Depressor and bradycardic responses to microinjections of endomorphin-2 into the NTS are mediated via ionotropic glutamate receptors
    Ken Kasamatsu
    Dept of Neurological Surgery, MSB H 586, UMDNJ New Jersey Medical School, 185 South Orange Ave, Newark, NJ 07103, USA
    Am J Physiol Regul Integr Comp Physiol 287:R715-28. 2004
    ..5 mmHg) and heart rate (50 +/- 7.0 beats/min). These responses were blocked by prior microinjections of naloxonazine (1 mmol/l) into the mNTS...
  78. ncbi Transgenesis in Drosophila melanogaster
    Leonie Ringrose
    IMBA Institute of Molecular Biotechnology GmbH, Vienna, Austria
    Methods Mol Biol 561:3-19. 2009
    ..This chapter gives a detailed protocol for microinjection, and aims to enable the reader to use it for both site-directed integration and for P-element transgenesis...
  79. ncbi Microporation applications for enhancing drug delivery
    Ajay K Banga
    Mercer University, College of Pharmacy and Health Sciences, Department of Pharmaceutical Sciences, Atlanta, GA 30341, USA
    Expert Opin Drug Deliv 6:343-54. 2009
    ..Bioavailability considerations and the potential use of inexpensive coated microneedles for mass immunizations are also discussed...
  80. pmc Subunit-specific contribution of pore-forming domains to NMDA receptor channel structure and gating
    Alexander I Sobolevsky
    Department of Neurobiology and Behavior, State University of New York at Stony Brook, Stony Brook, NY 11794, USA
    J Gen Physiol 129:509-25. 2007
    ....
  81. ncbi Microinjection of ANG II into paraventricular nucleus enhances cardiac sympathetic afferent reflex in rats
    Guo Qing Zhu
    Department of Physiology and Biophysics, University of Nebraska College of Medicine, Omaha, Nebraska 68198 4575, USA
    Am J Physiol Heart Circ Physiol 282:H2039-45. 2002
    ..These central effects of ANG II are mediated by AT(1) receptors...
  82. ncbi Efficiency of assisted oocyte activation as a solution for failed intracytoplasmic sperm injection
    Björn Heindryckx
    Department for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
    Reprod Biomed Online 17:662-8. 2008
    ..When MOAT reveals no activation deficiency in spermatozoa, AOA still allows for high fertilization and acceptable pregnancy rates. The obstetric and neonatal outcomes after AOA were normal as no malformations were observed...
  83. ncbi A novel positive allosteric modulator of the alpha7 neuronal nicotinic acetylcholine receptor: in vitro and in vivo characterization
    Raymond S Hurst
    Global Research and Development, Pfizer Inc, Groton, Connecticut 06340, USA
    J Neurosci 25:4396-405. 2005
    ..Together, these results suggest that PNU-120596 represents a new class of molecule that enhances alpha7 nAChR function and thus has the potential to treat psychiatric and neurological disorders...
  84. ncbi Localization of hindbrain glucoreceptive sites controlling food intake and blood glucose
    S Ritter
    Department of Veterinary Anatomy, Washington State University, Pullman, WA, USA
    Brain Res 856:37-47. 2000
    ..Localization of glucoreceptive sites will facilitate positive identification of glucoreceptor cells and the direct analysis of the neural mechanisms through which they influence food intake and metabolic responses...
  85. ncbi Activation of CB1 cannabinoid receptors in the dorsolateral periaqueductal gray reduces the expression of contextual fear conditioning in rats
    L B M Resstel
    Department of Pharmacology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Av Bandeirantes 3900, 14049 900 Ribeirao Preto, Sao Paulo, Brazil
    Psychopharmacology (Berl) 198:405-11. 2008
    ..It also activates the dorsolateral column of the periaqueductal gray (dlPAG). Microinjections of cannabinoid agonists into the dlPAG produced anxiolytic-like effects in the elevated plus maze, but the ..
  86. ncbi Norepinephrine release in medial amygdala facilitates activation of the hypothalamic-pituitary-adrenal axis in response to acute immobilisation stress
    S Ma
    Department of Pharmacology and Center for Biomedical Neuroscience, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    J Neuroendocrinol 17:22-8. 2005
    ..elevation of plasma ACTH concentration induced by acute immobilisation stress immediately following bilateral microinjections of alpha1- or beta-adrenergic receptor antagonists directly into MeA...
  87. ncbi In vivo assessment of safety of microneedle arrays in human skin
    Suzanne M Bal
    Department of Drug Delivery Technology, Leiden Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
    Eur J Pharm Sci 35:193-202. 2008
    ..This opens the opportunity for dermal and transdermal delivery of drugs and vaccines...
  88. ncbi Positive and negative motivation in nucleus accumbens shell: bivalent rostrocaudal gradients for GABA-elicited eating, taste "liking"/"disliking" reactions, place preference/avoidance, and fear
    Sheila M Reynolds
    Department of Psychology, University of Michigan, Ann Arbor, Michigan 48109 1109, USA
    J Neurosci 22:7308-20. 2002
    ..To further test the hypothesis that rostral shell muscimol microinjections produce positive motivational states, whereas caudal shell muscimol produces negative states, we measured ..
  89. pmc bcl-x is expressed in embryonic and postnatal neural tissues and functions to prevent neuronal cell death
    M Gonzalez-Garcia
    Department of Pathology and Anatomy, University of Michigan Medical School, Ann Arbor 48109, USA
    Proc Natl Acad Sci U S A 92:4304-8. 1995
    ..Thus, Bcl-x proteins appear to play an important role in the regulation of neuronal survival in the adult CNS...
  90. ncbi Effects on sleep of melanin-concentrating hormone (MCH) microinjections into the dorsal raphe nucleus
    Patricia Lagos
    Departamento de Fisiologia, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Brain Res 1265:103-10. 2009
    ..hypothalamic MCHergic neurons are active during rapid eye movements (REM) sleep, and that intraventricular microinjections of MCH induce slow wave sleep (SWS) and REM sleep...
  91. ncbi Contrasting effects of dopamine and glutamate receptor antagonist injection in the nucleus accumbens suggest a neural mechanism underlying cue-evoked goal-directed behavior
    Irene A Yun
    Neuroscience Graduate Program, University of California, San Francisco, CA, USA
    Eur J Neurosci 20:249-63. 2004
    ....
  92. ncbi Antinociceptive tolerance revealed by cumulative intracranial microinjections of morphine into the periaqueductal gray in the rat
    Michael M Morgan
    Department of Psychology, Washington State University Vancouver, Vancouver, WA 98686, USA
    Pharmacol Biochem Behav 85:214-9. 2006
    ..0 micro g. Repeated microinjections of saline into the PAG had no effect on nociception...
  93. pmc Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphase
    G J Gorbsky
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA
    J Cell Biol 141:1193-205. 1998
    ..Thus, in mammalian somatic cells, the spindle checkpoint appears to be a component of the timing mechanism for normal mitosis, blocking anaphase onset until all chromosomes are aligned at the metaphase plate...
  94. ncbi The effect of cell division on the cellular dynamics of microinjected DNA and dextran
    James J Ludtke
    Departments of Pediatrics and Medical Genetics, University of Wisconsin Madison, Madison, Wisconsin 53705, USA
    Mol Ther 5:579-88. 2002
    ..They showed that the bulk of large dextran, and similarly pDNA, was excluded from re-formed nuclei after mitosis, thereby limiting the effect of cell division on the nuclear entry of pDNA...
  95. ncbi High throughput easy microinjection with a single-cell manipulation supporting robot
    Hideaki Matsuoka
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    J Biotechnol 116:185-94. 2005
    ..The present results indicate that SMSR is a useful machine for the microinjection of specific genes and proteins in living cells to analyze their respective functions, which is an urgent and important subject in the post-genome era...
  96. ncbi Differential effects of bicuculline and muscimol microinjections into the nucleus basalis magnocellularis in taste and place aversive memory formation
    I Moron
    Department of Experimental Psychology and Physiology of Behavior, University of Granada, Campus Cartuja, Granada 18071, Spain
    Behav Brain Res 134:425-31. 2002
    ..However, when the rats were tested 24 h later, those injected with bicuculline or muscimol showed an impairment of the IA learning. The present results support a role of the GABAergic system in the consolidation process of IA learning...
  97. pmc Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis
    Anastasios Pavlopoulos
    Institute of Molecular Biology and Biotechnology, 711 10 Iraklio Crete, Greece
    Proc Natl Acad Sci U S A 102:7888-93. 2005
    ....
  98. ncbi The effects of histaminergic agents in the central amygdala of rats in the elevated plus-maze test of anxiety
    Mohammad Reza Zarrindast
    Department of Pharmacology, School of Medicine and Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran
    Behav Pharmacol 16:643-9. 2005
    ..5 microl bilateral. The results suggest that histamine may modulate anxiety via H1 but not H2 receptors in the rat central amygdala...
  99. ncbi HuC:Kaede, a useful tool to label neural morphologies in networks in vivo
    Tomomi Sato
    Laboratory for Developmental Gene Regulation, Brain Science Institute, RIKEN, Japan
    Genesis 44:136-42. 2006
    ..These data indicate that Kaede is a useful tool to selectively label neural networks in zebrafish...
  100. ncbi Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo
    A Blangy
    Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland
    Cell 83:1159-69. 1995
    ..These results indicate that HsEg5 is required for establishing a bipolar spindle and that p34cdc2 protein kinase directly regulates its localization...
  101. pmc Effects of stress and hippocampal NMDA receptor antagonism on recognition memory in rats
    Kevin B Baker
    Department of Psychology, Yale University, New Haven, Connecticut 06520 8205, USA
    Learn Mem 9:58-65. 2002
    ..Together, these results suggest that stress and intrahippocampal administration of APV affect recognition memory by influencing synaptic plasticity in the hippocampus...

Research Grants63

  1. Arcuate nucleus glutamatergic neurons modulate energy homeostasis
    Anthony N van den Pol; Fiscal Year: 2013
    ..This will be tested with fluorogold and recombinant pseudorabies virus microinjections into putative target regions...
  2. Ghrelin: Behavioral, Anatomical, and Physiological Components of Obesity
    BRETT JOHN WARREN TEUBNER; Fiscal Year: 2011
    ..To test the function of centrally-acting ghrelin, site-specific microinjections of ghrelin will be made into brain loci with high SNS outflow to BAT/WAT + GHS-R1a colocalization and measure ..
  3. Neurochemistry/physiology of proopiomelanocortin neurons
    MALCOLM JAMES LOW; Fiscal Year: 2013
    ..A novel retrograde tracing method involving site-specific microinjections of a canine adenoviral vector expressing Cre recombinase into mutant mice with a reversibly silenced POMC ..
  4. Neurotoxicity of Magnetic Nanoparticles
    Veronica Shubayev; Fiscal Year: 2010
    ..changes were seen in the corresponding sham procedures, control DMSA and dextran-coated iron oxide MNPs microinjections. Utilizing a combination of engineering and biological in vitro and in vivo approaches, this program aims to ..
  5. P450 Epoxygenase Mechanisms of Opioid Analgesia
    LINDSAY HOUGH; Fiscal Year: 2013
    ..acts in the ventrolateral periaqueductal gray, the rostral ventromedial medulla, and the spinal dorsal horn, microinjections of P450 inhibitors and morphine into these regions of the rat CNS will identify the P450-relevant sites...
  6. Regulation and Function of Germline RNP Granules
    JENNIFER SCHISA; Fiscal Year: 2010
    ..the role of polysome stability in RNP granule assembly will be determined using a combination of inhibitor microinjections and targeted RNAi experiments...
  7. Neuroendocrine Regulation of Adipocyte Metabolism
    Diego Perez-Tilve; Fiscal Year: 2013
    ..Specifically we will combine nucleus specific microinjections in the rat central nervous system with virus mediated gene disruption and mutated mouse models to clarify ..
  8. A CENTER OF MOLECULAR DEVELOPMENTAL HEMATOPOIESIS
    Stuart H Orkin; Fiscal Year: 2013
    ..Targeting Core that has supported investigators through education and consultation, oocyte and blastocyst microinjections, assistance in embryo and hematological phenotyping, and de novo ES cell derivation...
  9. Altering Energy Balance by Systemic Delivery of RNAi to the Neuroendocrine Brain
    Sergio R Ojeda; Fiscal Year: 2013
    ..Alternatively, microinjections of adeno-associated viruses (AAV) delivering siRNAs have been used to modify hypothalamic function in ..
  10. Mu-Opioid Effects on the Central Mechanisms that Control Breathing
    Edward J Zuperku; Fiscal Year: 2013
    ..1: To locate the region in the PB-KF area that produces opioid-induced bradypnea via DAMGO (5OR agonist) microinjections by monitoring changes in respiratory phase durations from the phrenic neurogram (PNG)...
  11. The role of dendrodendritic dopamine neurotransmission in methamphetamine abuse
    Michael J Beckstead; Fiscal Year: 2013
    ..clamp electrophysiology in brain slices with intravenous METH self-administration and VTA site-specific drug microinjections in mice...
  12. Neuropeptides, Social Stress and Drugs of Abuse
    Klaus A Miczek; Fiscal Year: 2013
    ..The proposed research employs discrete intracerebral microinjections to stimulate and block CRF-R1 in discrete neural regions, in vivo microdialysis for sampling extraneuronal ..
  13. Cardiovascular Regulation in Hindlimb Unweighted Rats
    Eileen M Hasser; Fiscal Year: 2010
    ..address these questions using whole animal studies in conscious and anesthetized control and HU rats (Fos, microinjections, extracellular recording), immunohistochemistry, studies in single dissociated spinally projecting RVLM and ..
  14. The impact of HPA axis dysregulation on the interoceptive effects of alcohol
    Joyce Besheer; Fiscal Year: 2013
    ..Third, using brain site-specific microinjections, studies will assess the functional involvement of mGluR5 in the expression of alcohol's interoceptive ..
  15. Central mechanisms of nicotine reinforcement and dependence
    George F Koob; Fiscal Year: 2012
    ..responses, and brain stimulation reward combined with biochemical studies, and systemic and intracerebral microinjections of specific neuropharmacological agents will be employed...
  16. GABAergic Mechanisms in the Modulation of Binge-Like Ethanol Intake in Mice
    STEPHEN LEE BOEHM; Fiscal Year: 2013
    ..In aims 1 and 2, mice will receive anterior or posterior intra-VTA, -NAcc shell, or -mPFC microinjections of zolpidem or 4,5,6,7-tetrahydroisoxazolo-[5,4- c]pyridin-3-ol (THIP), known to target synaptic and ..
  17. Isolating the Extinction Circuit for Cocaine Seeking
    Ryan T Lalumiere; Fiscal Year: 2013
    ..Rats will receive bilateral microinjections of either GABA agonists to inactivate the structure or the AMPA receptor potentiator PEPA to activate the ..
  18. Neurobiological Mechanisms of Antidepressant Effects
    David A Morilak; Fiscal Year: 2013
    ..and 5-HT2A serotonin receptors in mPFC in the beneficial effects of CBT will be investigated, using local microinjections into mPFC...
  19. Sex Specific Neuroanatomical Markers Of Vulnerability In Animal Model Of PTSD
    Rebecca M Shansky; Fiscal Year: 2013
    ..Using a combinatio of retrograde tracer and iontophoretic fluorescent microinjections, the dendritic morphology of mPFC neurons that project to the amygdala will be analyzed in animals that show ..
  20. Brain integration of adiposity and satiety signals in the control of food intake
    Diana Williams; Fiscal Year: 2009
    ..areas in which leptin detection is sufficient to enhance the responses to GLP-1 and CCK, using site-specific microinjections and gene therapy in normal rats and rats that lack leptin receptors due to genetic mutation...
  21. Anatomical and Physiological Characterization of the Thalamostriatal System
    KALYNDA GONZALES; Fiscal Year: 2012
    ..of TANs to electrical CM stimulation will be recorded electrophysiologically in monkeys, before and after microinjections of GABA receptor antagonists in the vicinity of the striatal recording sites to determine if local GABAergic ..
  22. Carbon Nanopipette Cellular Probe for Microinjections into Mammalian Cells
    CHAMIKA WANSAPURA; Fiscal Year: 2010
    ..Direct comparison of microinjection using CNP to commercially available glass micropipettes will allow advancement in single cell microinjection applications by replacing the conventional glass micropipettes with CNPs. ..
  23. Adolescent risk-taking, dopamine signaling, and cocaine: a vicious circle
    Marci R Mitchell; Fiscal Year: 2012
    ..in the preliminary data and Aim 2 between risky decision-making and dopaminergic markers, using intracerebral microinjections of drugs targeting these markers in brain regions of interest such as dorsal striatum...
  24. Genetic targeting of CGRP actions in a murine model of migraine
    ERIC ALAN KAISER; Fiscal Year: 2011
    ..To test this hypothesis, stereotactic microinjections of virus will be used to induce hRAMPI expression in targeted regions based on previous clinical findings and ..
  25. Molecular Mechanisms of Cellular Plasticity in a Mouse Model of Excessive Alcohol
    Igor Ponomarev; Fiscal Year: 2012
    ..him to sharpen his skills as a young investigator and to learn cellular neuroscience techniques such as brain microinjections and laser capture microdissection. These techniques will allow Dr...
  26. Brain integration of adiposity and satiety signals in the control of food intake
    Diana L Williams; Fiscal Year: 2011
    ..areas In which leptin detection Is sufficient to enhance the responses to GLP-1 and CCK, using site-speclfic microinjections and gene therapy In normal rats and rats that lack leptin receptors due to genetic mutation...
  27. Infralimbic Regulation of Memory Consolidation of Extinction of Cocaine-seeking
    Ryan T Lalumiere; Fiscal Year: 2010
    ..Rats will receive microinjections immediately AFTER extinction sessions on days 1-5 of extinction training...
  28. Effects of chronic nicotinic excitation on central glutamatergic control of breat
    JASON QUINN PILARSKI; Fiscal Year: 2010
    ..We will use a "split-bath" brainstem spinal cord preparation with and without pressure microinjections of glutamate receptors agonists and antagonists (Specific aim 1), as well as whole-cell patch clamp ..
  29. Cortical connections with the limbic territory of the basal ganglia
    Michelle Morrow; Fiscal Year: 2007
    ..First, we will behaviorally define specific sites in the limbic territory of GPe using Bic microinjections. Then, we will inject rabies virus in GPe and allow retrograde transneuronal transport to 2nd order neurons ..
  30. Cortical contributions to basal ganglia disorders
    PETER STRICK; Fiscal Year: 2006
    ..To achieve this goal we will employ a unique multi-disciplinary approach. We will make microinjections of a GABAergic antagonist, bicuculline, into the external segment of the globus pallidus (GPE)...
  31. Accumbens coding of reward expectation: electrophysiology and neuropharmacology
    JON CRAIG HORVITZ; Fiscal Year: 2011
    ..single-neuron recordings in the accumbens core or shell will be obtained in parallel with intra-accumbens microinjections of DA antagonists, employing identical behavioral paradigms...
  32. Mechanisms of cue-induced reinstatement of cocaine seeking.
    Vidhya Kumaresan; Fiscal Year: 2012
    ..Effect of intra-accumbens microinjections of a GRIP-specific peptide, TVRTYSC (Dickinson et al...
  33. Context and Relapse to Ethanol-Seeking
    Patricia H Janak; Fiscal Year: 2013
    ..underlying the modulation of cue-induced alcohol-seeking by context using techniques of site-specific microinjections and in vivo electrophysiology...
  34. Closed-loop distributed microstimulation for epilepsy
    John D Rolston; Fiscal Year: 2010
    ..arrays, chronically implanted in the hippocampi or sensorimotor cortex of adult rats, made epileptic with microinjections of tetanus toxin in the same region...
  35. The neurobiology of cue-induced relapse to ethanol-seeking behavior in mice
    Peter A Groblewski; Fiscal Year: 2010
    ..expression of cue-induced relapse by manipulating the neuronal activity of these regions using site-specific microinjections of lidocaine...
  36. PKMzeta involvement in cocaine sensitization
    CARLOS A JIMENEZ-RIVERA; Fiscal Year: 2013
    ..Second, we will use microinjections of the synthetic peptide Tat-GluR23y and rectification index curves in whole cell patch recordings to examine ..
  37. Role of Amygdalar CRF Receptors in Regulating Anxiety
    Tammy Sajdyk; Fiscal Year: 2005
    ..b>Microinjections of both agonists and antagonists of CRF receptors will be given into the BLA...
  38. VTA Glutamate and Orexin Involvement in Cue Reinstatement of Drug Seeking
    STEPHEN VINCENT MAHLER; Fiscal Year: 2012
    ..to VTA necessary for CS reinstatement? To address these questions, we use Fos and tract tracing techniques, microinjections of orexin and glutamate antagonists in VTA, and simultaneous inactivation of mPFC and contralateral ..
  39. Cellular and Molecular Mechanisms of REM Sleep Control
    James Quattrochi; Fiscal Year: 2005
    ..Third, the effects on LTRE/LTPE following suppression of Fos protein in the PMT will be studied by performing microinjections of c-fos antisense oligonucleotides...
  40. Anesthetics, GABA and the Injured Brain
    David Warner; Fiscal Year: 2006
    ..For Specific Aim #4, we will examine: a) if isoflurane protection against forebrain ischemia or striatal NMDA microinjections is reversed by GABAA antagonists (flurothyl, bicuculline, flumazenil), b) if isoflurane delays time to ..
  41. Involvement of Aldehydes in Alcohol Addiction
    William McBride; Fiscal Year: 2009
    ..Aim 2 will determine the effects of local microinjections of a catalase inhibitor (3-amino-1, 2,4-triazole [tnazole]), ACD or SAL into the posterior ventral tegmental ..
  42. FORE-SCI TRAINING PROGRAM (RFP 08-01)
    DANA MCTIGUE; Fiscal Year: 2011
    ..readouts (including EMGs, MEPs, and SEPs), methods for intraspinal delivery of drugs and/or cells including microinjections and intrathecal catheter delivery...
  43. BRAIN REGULATING GASTRIC ACID FUNCTION BY NEUROPEPTIDES
    YVETTE TACHE; Fiscal Year: 2000
    ..These aims will be achieved by combined methods of approach: neuropharmacological (microinjections of TRH antibody, selective SST ligands), neurohistochemical (c-fos expression in medullary nuclei as a marker ..
  44. FORE-SCI TRAINING PROGRAM (RFP 08-01)
    Dana M McTigue; Fiscal Year: 2009
    ..readouts (including EMGs, MEPs, and SEPs), methods for intraspinal delivery of drugs and/or cells including microinjections and intrathecal catheter delivery...
  45. FORE-SCI TRAINING PROGRAM (RFP 08-01)
    DANA MCTIGUE; Fiscal Year: 2012
    ..readouts (including EMGs, MEPs, and SEPs), methods for intraspinal delivery of drugs and/or cells including microinjections and intrathecal catheter delivery...
  46. Cue-triggered reward seeking
    Kent C Berridge; Fiscal Year: 2012
    ..In experiment 1, microinjections of agonist drugs and a Fos plume mapping tool will be used to identify the neural mechanisms in nucleus ..
  47. Cellular Mechanisms of Opioid Tolerance
    Michael M Morgan; Fiscal Year: 2010
    ..will be tested by determining whether mu-opioid receptor internalization contributes to tolerance to morphine microinjections into the PAG...
  48. NEURONAL POLARITY
    KENNETH KOSIK; Fiscal Year: 2000
    ..that lacks a major actin filament gelation protein, actin binding protein (ABP-280) and the effects of MAP2c microinjections. These cells show defects in cortical gelation that are visibly corrected by the microinjection of actin ..
  49. PREOPTIC AREA AND PHARMACOLOGIC SLEEP INDUCTION
    Paul Vezina; Fiscal Year: 2001
    ..from the investigator's laboratory and those from other laboratories indicating similar effects from microinjections of ethanol and adenosine...
  50. Instrument for Intracerebral Microinjections and Electrophysiology
    Lee Margolin; Fiscal Year: 2009
    ..The Intracerebral Microinjection Instrument proposed herein improves the ability to make such injections with minimal damage to the brain, and with minimal activation of the brains rejection mechanisms for foreign material. ..
  51. Basal ganglia discharge patterns in parkinsonism
    Thomas Wichmann; Fiscal Year: 2005
    ..A. 2 by studying changes in oscillatory activity in basal ganglia and cortex induced by microinjections of the dopamine receptor agonist apomorphine at striatal and extrastriatal basal ganglia sites in ..
  52. Retrograde amnesia after hippocampal damage
    Robert Sutherland; Fiscal Year: 2006
    ..of retrograde amnesia produced by nearly complete, selective hippocampal formation damage caused by multiple microinjections of N-methyl-Daspartate...
  53. ROLE OF NUCLEUS ACCUMBENS CA2+ FLUX IN COCAINE REWARD
    WILLIAM CARLEZON; Fiscal Year: 2000
    ..We will precede treatment with a threshold dose of cocaine (1.25 mg/kg, IP) with intra-NASh microinjections of an L-type Ca2+ channel antagonist (diltiazem) or an activator (BAY k 8644) during drug conditioning ..
  54. NEURAL SIGNALLING OF SALT INTAKE
    ALEX BAERTSCHI; Fiscal Year: 1993
    ..Objective 3 is to determine the brain nuclei involved in splanchnic osmosensation by microinjections of ibotenic acid into the crucial cell groups. This neurotoxin impairs cell bodies but not axons in passage...
  55. VAGAL GASTRIC MOTOR CONTROL IN MICE
    ELITA PARTOSEOEDARSO; Fiscal Year: 2002
    ..Therefore, in this exploratory proposal we will modify our physiological recordings and stereotaxic brain microinjections for use in mice. This would be a unique approach in the field of brain-gut interactions...
  56. NEURAL TRANSPLANTS, BEHAVIOR & HUNTINGTON'S MODEL
    Paul Sanberg; Fiscal Year: 1990
    b>Microinjections of kainic acid (KA) into the striatum of experimental animals result in degeneration of local intrinsic as well as efferent neurons, leaving axons and terminals of extrinsic neurons intact...
  57. HINDBRAIN AND NEUROPEPTIDE Y--CONTROL OF FOOD INTAKE
    ERIC CORP; Fiscal Year: 2002
    ..This locus, we predict, will be more sensitive than other hindbrain sites to microinjections of an NPY- related agonist...
  58. INTERACTIONS BETWEEN F-ACTIN, MYOSIN II AND MICROTUBULES
    Garrett Odell; Fiscal Year: 2001
    ..effect mutations gnu (giant nuclei), mav (mavericks), and sponge as well as perturbations caused by drug microinjections. These will be analyzed to help verify or reject competing hypotheses about what mechanistic interactions ..
  59. LOCUS COERULEUS AND ATTENTIONAL PROCESSING
    Gary Aston Jones; Fiscal Year: 1999
    ..This will allow analysis of LC's involvement in attentional lability. (2) Local microinjections of selective pharmacologic agents into the LC will be used to transiently and specifically inactivate or ..
  60. NEURONAL CYTOSKELETAL REMODELLING
    PETER MORIN; Fiscal Year: 1999
    ..M2) lacking a major actin filament gelation protein, actin binding protein (ABP-280) can be rescued by MAP2c microinjections. These cells show defects in cortical gelation that are visibly corrected by microinjection of actin gelation ..
  61. Role of TrkB and TrkC in Cocaine-Induced Sensitization
    AUSAF BARI; Fiscal Year: 2006
    ..BDNF and NT-3 microinjections into the VTA have been shown to enhance the behavioral activating effects of cocaine...
  62. Abnormal Olivocerebellar Synchrony: A Possible Cause of Alcohol Withdrawal Tremor
    ERIC LANG; Fiscal Year: 2009
    ..this end the characteristics of olivocerebellar activity will be pharmacologically manipulated using direct microinjections into the IO while EMG recordings are being made of the tremor in order to assess the resulting changes in ..
  63. NEUROPHYSIOLOGICAL & MODELING STUDIES OF LOCUS COERULEUS
    Gary Aston Jones; Fiscal Year: 2000
    ..3) The investigators will make local microinjections of selective pharmacologic agents into the monkey LC to transiently manipulate activity of, and electrotonic ..