microfluidic analytical techniques

Summary

Summary: Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.

Top Publications

  1. ncbi Dynamic single cell culture array
    Dino Di Carlo
    Berkeley Sensor and Actuator Center, Biomolecular Nanotechnology Center, Department of Bioengineering, University of California, Berkeley, CA 94720, USA
    Lab Chip 6:1445-9. 2006
  2. pmc Isolation of circulating tumor cells using a microvortex-generating herringbone-chip
    Shannon L Stott
    Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 107:18392-7. 2010
  3. ncbi A systems approach to measuring the binding energy landscapes of transcription factors
    Sebastian J Maerkl
    Biochemistry and Molecular Biophysics Option, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Science 315:233-7. 2007
  4. pmc Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines
    Ashley A Powell
    Department of Surgery, Stanford University School of Medicine, Stanford, California, USA
    PLoS ONE 7:e33788. 2012
  5. ncbi Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation
    Ali Asgar S Bhagat
    BioSystems and Micromechanics IRG, Singapore MIT Alliance for Research and Technology Centre, Singapore
    Lab Chip 11:1870-8. 2011
  6. ncbi Quantitative and sensitive detection of rare mutations using droplet-based microfluidics
    Deniz Pekin
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, BP 70028, F 67083 Strasbourg cedex, France
    Lab Chip 11:2156-66. 2011
  7. ncbi Soft lithography for micro- and nanoscale patterning
    Dong Qin
    Nano Research Facility and Department of Energy, Environmental, and Chemical Engineering, Washington University, St Louis, Missouri, USA
    Nat Protoc 5:491-502. 2010
  8. pmc A microfluidic culture platform for CNS axonal injury, regeneration and transport
    Anne M Taylor
    Department of Biomedical Engineering, University of California, Irvine, 204 Rockwell Engineering, Irvine, California 92697, USA
    Nat Methods 2:599-605. 2005
  9. ncbi Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans
    Nikos Chronis
    Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nat Methods 4:727-31. 2007
  10. ncbi Deformability based cell margination--a simple microfluidic design for malaria-infected erythrocyte separation
    Han Wei Hou
    Division of Bioengineering, National University of Singapore, 7 Engineering Drive 1, Singapore, 117574, Singapore
    Lab Chip 10:2605-13. 2010

Detail Information

Publications418 found, 100 shown here

  1. ncbi Dynamic single cell culture array
    Dino Di Carlo
    Berkeley Sensor and Actuator Center, Biomolecular Nanotechnology Center, Department of Bioengineering, University of California, Berkeley, CA 94720, USA
    Lab Chip 6:1445-9. 2006
    ..We anticipate uses in single cell analysis of drug toxicity with physiologically relevant perfused dosages as well as investigation of cell signaling pathways and systems biology...
  2. pmc Isolation of circulating tumor cells using a microvortex-generating herringbone-chip
    Shannon L Stott
    Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 107:18392-7. 2010
    ..In a subset of patient samples, the low shear design of the HB-Chip revealed microclusters of CTCs, previously unappreciated tumor cell aggregates that may contribute to the hematogenous dissemination of cancer...
  3. ncbi A systems approach to measuring the binding energy landscapes of transcription factors
    Sebastian J Maerkl
    Biochemistry and Molecular Biophysics Option, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Science 315:233-7. 2007
    ..With this platform we characterized DNA binding energy landscapes for four eukaryotic transcription factors; these landscapes were used to test basic assumptions about transcription factor binding and to predict their in vivo function...
  4. pmc Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines
    Ashley A Powell
    Department of Surgery, Stanford University School of Medicine, Stanford, California, USA
    PLoS ONE 7:e33788. 2012
    ..Here we demonstrate feasibility of performing high dimensional single CTC profiling, providing early insight into CTC heterogeneity and allowing comparisons to breast cancer cell lines widely used for drug discovery...
  5. ncbi Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation
    Ali Asgar S Bhagat
    BioSystems and Micromechanics IRG, Singapore MIT Alliance for Research and Technology Centre, Singapore
    Lab Chip 11:1870-8. 2011
    ....
  6. ncbi Quantitative and sensitive detection of rare mutations using droplet-based microfluidics
    Deniz Pekin
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, BP 70028, F 67083 Strasbourg cedex, France
    Lab Chip 11:2156-66. 2011
    ....
  7. ncbi Soft lithography for micro- and nanoscale patterning
    Dong Qin
    Nano Research Facility and Department of Energy, Environmental, and Chemical Engineering, Washington University, St Louis, Missouri, USA
    Nat Protoc 5:491-502. 2010
    ....
  8. pmc A microfluidic culture platform for CNS axonal injury, regeneration and transport
    Anne M Taylor
    Department of Biomedical Engineering, University of California, Irvine, 204 Rockwell Engineering, Irvine, California 92697, USA
    Nat Methods 2:599-605. 2005
    ..The results presented here demonstrate several experimental paradigms using the microfluidic platform, which can greatly facilitate future studies in axonal biology...
  9. ncbi Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans
    Nikos Chronis
    Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nat Methods 4:727-31. 2007
    ..The use of these chips can be extended to correlate the activity of sensory neurons, interneurons and motor neurons with the worm's behavior...
  10. ncbi Deformability based cell margination--a simple microfluidic design for malaria-infected erythrocyte separation
    Han Wei Hou
    Division of Bioengineering, National University of Singapore, 7 Engineering Drive 1, Singapore, 117574, Singapore
    Lab Chip 10:2605-13. 2010
    ..g. sickle cell anemia and leukemia, characterized by changes in cell stiffness...
  11. pmc Microfluidics separation reveals the stem-cell-like deformability of tumor-initiating cells
    Weijia Zhang
    Department of Nanomedicine and Methodist Cancer Center, Methodist Hospital Research Institute, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 109:18707-12. 2012
    ..Our observations support the relationship between tumor-initiating capacity and cell deformability, and demonstrate that tumor-initiating cells are less differentiated in terms of cell biomechanics...
  12. ncbi Surface acoustic wave actuated cell sorting (SAWACS)
    T Franke
    Department of Physics and School of Engineering and Applied Science, Harvard University, Cambridge, USA
    Lab Chip 10:789-94. 2010
    ..We have successfully directed HaCaT cells (human keratinocytes), fibroblasts from mice and MV3 melanoma cells. The low shear forces of this sorting method ensure that cells survive after sorting...
  13. pmc De novo identification and biophysical characterization of transcription-factor binding sites with microfluidic affinity analysis
    Polly M Fordyce
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA
    Nat Biotechnol 28:970-5. 2010
    ..We expect that these data and future use of this technique will provide information essential for understanding transcription factor specificity, improving identification of regulatory sites and reconstructing regulatory interactions...
  14. ncbi Microdevice for the isolation and enumeration of cancer cells from blood
    Swee Jin Tan
    Institute of Microelectronics, A STAR Agency for Science, Technology and Research, 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore
    Biomed Microdevices 11:883-92. 2009
    ..The microdevice has the potential to be used for routine monitoring of cancer development and cancer therapy in a clinical setting...
  15. pmc A microfluidic processor for gene expression profiling of single human embryonic stem cells
    Jiang F Zhong
    Department of Neurology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Lab Chip 8:68-74. 2008
    ..The results underscore the importance of discrete single-cell analysis, and the advantages of a microfluidic approach in stem cell gene expression studies...
  16. pmc Functional characterization of circulating tumor cells with a prostate-cancer-specific microfluidic device
    Brian J Kirby
    Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, New York, USA
    PLoS ONE 7:e35976. 2012
    ....
  17. pmc Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture (GEDI) and a prostate-specific antibody
    Jason P Gleghorn
    Sibley School of Mechanical and Aerospace Engineering, College of Engineering, Cornell University, Ithaca, NY 14853, USA
    Lab Chip 10:27-9. 2010
    ....
  18. ncbi Droplet based microfluidics
    Ralf Seemann
    Experimental Physics, Saarland University, D 66123 Saarbrucken, Germany
    Rep Prog Phys 75:016601. 2012
    ..Examples of applications and reaction schemes which rely on the discussed manipulation techniques are also presented, such as the fabrication of special materials and biophysical experiments...
  19. ncbi An integrated microfluidic system for rapid diagnosis of dengue virus infection
    Yu Fang Lee
    Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan
    Biosens Bioelectron 25:745-52. 2009
    ..This integrated system may provide a powerful platform for the rapid diagnosis of dengue virus infection and other types of infectious diseases...
  20. ncbi An isothermal amplification reactor with an integrated isolation membrane for point-of-care detection of infectious diseases
    Changchun Liu
    Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, 19104 6315, USA
    Analyst 136:2069-76. 2011
    ..The cassette can be readily modified to detect nucleic acids associated with other pathogens borne in saliva, urine, and other body fluids as well as in water and food...
  21. ncbi 1-Million droplet array with wide-field fluorescence imaging for digital PCR
    Andrew C Hatch
    Department of Biomedical Engineering, University of California Irvine, Irvine, CA 92697, USA
    Lab Chip 11:3838-45. 2011
    ..Compared to previous work, this highly integrated design yields a 100-fold increase in the number of on-chip digitized reactors with simultaneous fluorescence imaging for digital PCR based assays...
  22. pmc Micromagnetic selection of aptamers in microfluidic channels
    Xinhui Lou
    Department of Materials, University of California, Santa Barbara, CA 93106, USA
    Proc Natl Acad Sci U S A 106:2989-94. 2009
    ....
  23. ncbi Inertial microfluidics
    Dino Di Carlo
    Department of Bioengineering and California NanoSystems Institute, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, CA 90095, USA
    Lab Chip 9:3038-46. 2009
    ..Finally, since this a nascent area of research, I will suggest some future promising directions exploiting fluid inertia on the microscale...
  24. ncbi Microfluidic large-scale integration: the evolution of design rules for biological automation
    Jessica Melin
    Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, Stanford, California 94305, USA
    Annu Rev Biophys Biomol Struct 36:213-31. 2007
    ..Several microfluidic components used as building blocks to create effective, complex, and highly integrated microfluidic networks are also highlighted...
  25. pmc Using buoyant mass to measure the growth of single cells
    Michel Godin
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, USA
    Nat Methods 7:387-90. 2010
    ..We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells...
  26. pmc Biological implications of polydimethylsiloxane-based microfluidic cell culture
    Keil J Regehr
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53706 1609, USA
    Lab Chip 9:2132-9. 2009
    ..These findings highlight the importance of careful consideration of culture system properties when determining an appropriate environment for biological experiments...
  27. ncbi Power-free poly(dimethylsiloxane) microfluidic devices for gold nanoparticle-based DNA analysis
    Kazuo Hosokawa
    Bioengineering Laboratory, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Lab Chip 4:181-5. 2004
    ..The channel layout enabled two simultaneous DNA analyses at the two interfaces between the three laminar streams...
  28. ncbi Engineers are from PDMS-land, Biologists are from Polystyrenia
    Erwin Berthier
    Department of Medical Microbiology, University of Wisconsin Madison, Madison, WI, USA
    Lab Chip 12:1224-37. 2012
    ....
  29. pmc High-performance single cell genetic analysis using microfluidic emulsion generator arrays
    Yong Zeng
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Anal Chem 82:3183-90. 2010
    ..This result demonstrates that multiplex SCGA is a promising tool for high-throughput quantitative digital analysis of genetic variation in complex populations...
  30. ncbi An integrated microfluidic culture device for quantitative analysis of human embryonic stem cells
    Ken ichiro Kamei
    Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA
    Lab Chip 9:555-63. 2009
    ....
  31. pmc Single-cell protein induction dynamics reveals a period of vulnerability to antibiotics in persister bacteria
    Orit Gefen
    Racah Institute for Physics and Center for NanoScience and Nanotechnology, Hebrew University, Jerusalem 91904, Israel
    Proc Natl Acad Sci U S A 105:6145-9. 2008
    ....
  32. pmc Aptamer-enabled efficient isolation of cancer cells from whole blood using a microfluidic device
    Weian Sheng
    Department of Mechanical and Aerospace Engineering, University of Florida, P O Box 116250, Gainesville, Florida 32611, USA
    Anal Chem 84:4199-206. 2012
    ..In addition, we found that ~93% of the captured cells were viable, making them suitable for subsequent molecular and cellular studies...
  33. pmc Microfluidic fluorescence in situ hybridization and flow cytometry (μFlowFISH)
    Peng Liu
    Biotechnology and Bioengineering Department, Sandia National Laboratories, PO Box 969, MS 9291, Livermore, CA 94550, USA
    Lab Chip 11:2673-9. 2011
    ....
  34. ncbi A microfluidic-based device for study of transendothelial invasion of tumor aggregates in realtime
    Qian Zhang
    Section of Oral Pathology, College of Stomatology, Dalian Medical University, China
    Lab Chip 12:2837-42. 2012
    ....
  35. ncbi Performance of an organic photodiode as an optical detector and its application to fluorometric flow-immunoassay for IgA
    Mayo Miyake
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi ku, Fukuoka 819 0395, Japan
    Talanta 96:132-9. 2012
    ..The detection limits for resorufin and IgA were 5.0μM and 16ng/mL, respectively. The photosensitivity of the OPD remained relatively constant for a minimum of one year...
  36. ncbi Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS)
    Ivan K Dimov
    Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland
    Lab Chip 11:845-50. 2011
    ....
  37. ncbi Selection and elution of aptamers using nanoporous sol-gel arrays with integrated microheaters
    Seung Min Park
    Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Lab Chip 9:1206-12. 2009
    ..To our knowledge, this is the first demonstration of a chip-based selection of aptamers using microfluidics, thereby allowing development of a high throughput and efficient SELEX procedures...
  38. pmc A parallel microfluidic flow cytometer for high-content screening
    Brian K McKenna
    Boston University, Department of Biomedical Engineering, Boston, Massachusetts, USA
    Nat Methods 8:401-3. 2011
    ....
  39. pmc An on-chip, multichannel droplet sorter using standing surface acoustic waves
    Sixing Li
    Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA
    Anal Chem 85:5468-74. 2013
    ....
  40. ncbi High-throughput and high-resolution flow cytometry in molded microfluidic devices
    Claire Simonnet
    Department of Physics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Anal Chem 78:5653-63. 2006
    ..The achieved resolution is comparable to that of still micrographs. This high-resolution device could be used for analysis of morphology and fluorescence distribution in cells in continuous flow...
  41. ncbi An agar gel membrane-PDMS hybrid microfluidic device for long term single cell dynamic study
    Ieong Wong
    Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, CA 90095, USA
    Lab Chip 10:2710-9. 2010
    ..coli isobutanol tolerant strains. We believe this novel device will bring new capabilities to quantitative microbiology, providing a versatile platform for single cell dynamic studies...
  42. pmc Acoustophoretic sorting of viable mammalian cells in a microfluidic device
    Allen H J Yang
    Institute for Collaborative Biotechnologies, University of California, Santa Barbara, California 93106, USA
    Anal Chem 84:10756-62. 2012
    ..We have extensively characterized the device, and we report the effects of piezoelectric voltage and sample flow rate on device performance and describe how these parameters can be tuned to optimize recovery, purity, or throughput...
  43. pmc Advances in microfluidic PCR for point-of-care infectious disease diagnostics
    Seungkyung Park
    Department of Emergency Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    Biotechnol Adv 29:830-9. 2011
    ..In this review, we provide an overview of recent advances in microfluidic PCR technologies and discuss practical issues and perspectives related to implementing them into infectious disease diagnostics...
  44. pmc Aptamer-based microfluidic device for enrichment, sorting, and detection of multiple cancer cells
    Ye Xu
    Center for Research at the Bio Nano Interface, Department of Chemistry, Shands Cancer Center, UF Genetics Institute, University of Florida, Gainesville, Florida 32611 7200, USA
    Anal Chem 81:7436-42. 2009
    ....
  45. ncbi A self-contained, programmable microfluidic cell culture system with real-time microscopy access
    Peder Skafte-Pedersen
    Department of Micro and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345B, DK 2800, Kongens Lyngby, Denmark
    Biomed Microdevices 14:385-99. 2012
    ..Applicability for biological assays is demonstrated by on-chip cell culture including on-chip transfection and temporally programmable gene expression...
  46. ncbi In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system
    Rui Lima
    Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, 6 6 01 Aoba, 980 8579, Sendai, Japan
    Biomed Microdevices 10:153-67. 2008
    ..Advantages and disadvantages of PDMS microchannels over glass capillaries are also discussed...
  47. ncbi Co-pathological connected primary neurons in a microfluidic device for Alzheimer studies
    Anja Kunze
    Laboratoire de Microsystèmes LMIS4, Ecole Polytechnique Federale de Lausanne, Station 17, CH 1015 Lausanne, Switzerland
    Biotechnol Bioeng 108:2241-5. 2011
    ..g., in Alzheimer), as well as simultaneous drug effects on connected healthy and diseased cell populations...
  48. pmc An in vitro microfluidic approach to generating protein-interaction networks
    Doron Gerber
    Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, 318 Campus Drive, Stanford, CA 94305, USA
    Nat Methods 6:71-4. 2009
    ..The resulting network of 157 interactions was denser than expected based on known networks. Analysis of the network revealed previously undescribed physical interactions among members of some biochemical pathways...
  49. ncbi Microfluidic controlling monodisperse microdroplet for 5-fluorouracil loaded genipin-gelatin microcapsules
    Keng Shiang Huang
    Dept of Biomedical Engineering, I Shou University, Taiwan
    J Control Release 137:15-9. 2009
    ..The proposed microfluidic chip is capable of generating relatively uniform micro-droplets with well controllable diameter, and it has the added advantages of being a simple, low cost, and high throughput process...
  50. ncbi Cell lysis and DNA extraction of gram-positive and gram-negative bacteria from whole blood in a disposable microfluidic chip
    Madhumita Mahalanabis
    Department of Biomedical Engineering, Boston University, Boston, MA 02115, USA
    Lab Chip 9:2811-7. 2009
    ..Total extraction times are less than one hour and can be further decreased by altering the channel geometry and pumping configuration...
  51. ncbi Epidermal growth factor promotes breast cancer cell chemotaxis in CXCL12 gradients
    Bobak Mosadegh
    Department of Biomedical Engineering, 3120 Natural Sciences II, University of California at Irvine, Irvine, California 92697, USA
    Biotechnol Bioeng 100:1205-13. 2008
    ..This dependence on growth factor signaling points to the importance of autocrine and paracrine factors in determining the migratory response of the cells, and may play an important role in cancer metastasis...
  52. ncbi Valve-based microfluidic compression platform: single axon injury and regrowth
    Suneil Hosmane
    Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Lab Chip 11:3888-95. 2011
    ..At severe levels of injury (> 95 kPa), virtually all axons were instantaneously transected and nearly half (~46%) of these axons were able to regrow within the imaging period in the absence of exogenous stimulating factors...
  53. ncbi Quantitative cell-based reporter gene assays using droplet-based microfluidics
    Jean Christophe Baret
    Institut de Science et d Ingenierie Supramoleculaires, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, BP 70028, F 67083 Strasbourg cedex, France
    Chem Biol 17:528-36. 2010
    ..Screening approximately 7500 cells per concentration provides statistically relevant data that allow precise measurement of the EC(50) (70 nM +/- 12%, alpha = 0.05), in agreement with standard methods as well as with literature data...
  54. pmc Probing the limits of aptamer affinity with a microfluidic SELEX platform
    Kareem M Ahmad
    Interdepartmental Program in Biomolecular Science and Engineering, University of California Santa Barbara, Santa Barbara, California, United States of America
    PLoS ONE 6:e27051. 2011
    ..To further explore this relationship, we performed selections against PDGF-BB under different pH conditions using the same selection protocol, and report an inverse correlation between protein charge and aptamer K(d)...
  55. ncbi Long-term high-resolution imaging and culture of C. elegans in chip-gel hybrid microfluidic device for developmental studies
    Jan Krajniak
    School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
    Lab Chip 10:1862-8. 2010
    ..We show here that this method facilitates time-lapse studies of single animals at high-resolution and lends itself to live imaging experiments on developmental processes and dynamic events...
  56. pmc High-throughput injection with microfluidics using picoinjectors
    Adam R Abate
    School of Engineering and Applied Sciences and Department of Physics, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 107:19163-6. 2010
    ..Here, we introduce the picoinjector, a robust device to add controlled volumes of reagent using electro-microfluidics at kilohertz rates. It can also perform multiple injections for serial and combinatorial additions...
  57. ncbi Acoustofluidics 12: Biocompatibility and cell viability in microfluidic acoustic resonators
    Martin Wiklund
    Department of Applied Physics, Royal Institute of Technology, SE 10691, Stockholm, Sweden
    Lab Chip 12:2018-28. 2012
    ..This tutorial discusses the biocompatibility of devices designed for acoustic manipulation of mammalian cells, and different methods for quantifying the cell viability in such devices...
  58. ncbi Acceleration of emergence of bacterial antibiotic resistance in connected microenvironments
    Qiucen Zhang
    Department of Physics, Princeton University, Princeton, NJ 08544, USA
    Science 333:1764-7. 2011
    ..Knowledge about the rapid emergence of antibiotic resistance in the heterogeneous conditions within the mammalian body may be helpful in understanding the emergence of drug resistance during cancer chemotherapy...
  59. pmc Continuous-flow enzyme assay on a microfluidic chip for monitoring glycerol secretion from cultured adipocytes
    Anna M Clark
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Anal Chem 81:2350-6. 2009
    ..The ability to monitor changes in cellular secretion over time may provide insight into adipocyte metabolism and the dysregulation that occurs with obesity-related disorders...
  60. pmc Studying copy number variations using a nanofluidic platform
    Jian Qin
    Fluidigm Corporation, South San Francisco, CA 94080, USA
    Nucleic Acids Res 36:e116. 2008
    ..The use of the digital array enables accurate measurement of gene copy numbers and is of significant value in CNV studies...
  61. pmc Wide field-of-view lens-free fluorescent imaging on a chip
    Ahmet F Coskun
    Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA
    Lab Chip 10:824-7. 2010
    ....
  62. ncbi Better shrinkage than Shrinky-Dinks
    Diep Nguyen
    Department of Biomedical Engineering, UC Irvine, USA
    Lab Chip 10:1623-6. 2010
    ..In this technical note, we demonstrate the ability to rapidly and easily create unique microstructures, increase microarray feature density, and even induce self-assembled integrated metallic nanostructures with these shrink wrap films...
  63. ncbi Chemoattraction to dimethylsulfoniopropionate throughout the marine microbial food web
    Justin R Seymour
    Ralph M Parsons Laboratory, Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 329:342-5. 2010
    ....
  64. ncbi Cell migration into scaffolds under co-culture conditions in a microfluidic platform
    Seok Chung
    Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    Lab Chip 9:269-75. 2009
    ....
  65. pmc Conformational analysis of single DNA molecules undergoing entropically induced motion in nanochannels
    J T Mannion
    School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA
    Biophys J 90:4538-45. 2006
    ..Finally, we demonstrate that we can use a controlled applied field and the entropic interfacial forces to unfold molecules, which can then be manipulated and positioned in their simple extended morphology...
  66. pmc Microfluidics for the analysis of behavior, nerve regeneration, and neural cell biology in C. elegans
    Adela Ben-Yakar
    Department of Mechanical Engineering, University of Texas at Austin, 204 E Dean Keeton Street, Austin, TX 78705, USA
    Curr Opin Neurobiol 19:561-7. 2009
    ..elegans nervous system, extending from fundamental understanding of behavioral dynamics to more complicated biological processes such as neural aging and learning and memory...
  67. pmc Microfluidics meet cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays
    Amy L Paguirigan
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Bioessays 30:811-21. 2008
    ..With more validation and improved designs, microscale techniques hold immense promise as a platform to study aspects of cell biology that are not possible using current macroscale techniques...
  68. ncbi Cell research with physically modified microfluidic channels: a review
    Sun Min Kim
    Department of Mechanical Engineering, Inha University, 253 Young Hyun dong, Nam Gu, Incheon 402 751, Republic of Korea
    Lab Chip 8:1015-23. 2008
    ..Throughout this review, we will specify examples where necessary and provide trends and future directions in the field...
  69. ncbi In vitro analysis of a hepatic device with intrinsic microvascular-based channels
    Amedeo Carraro
    Department of Surgery, Massachusetts General Hospital, Boston, MA, USA
    Biomed Microdevices 10:795-805. 2008
    ..The design and fabrication processes are scalable, enabling the device concept to serve as both a platform technology for drug discovery and toxicity, and for the continuing development of an improved liver-assist device...
  70. pmc Optofluidic fluorescent imaging cytometry on a cell phone
    Hongying Zhu
    Electrical Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, USA
    Anal Chem 83:6641-7. 2011
    ..g., toward monitoring of HIV+ patients) or rare cell analysis as well as for screening of water quality in remote and resource-poor settings...
  71. ncbi An automated microfluidic platform for calcium imaging of chemosensory neurons in Caenorhabditis elegans
    Trushal Vijaykumar Chokshi
    Department of Electrical and Computer Engineering, University of Michigan, 1301 Beal Avenue, Ann Arbor, MI 48109, USA
    Lab Chip 10:2758-63. 2010
    ....
  72. ncbi Entrainment of a population of synthetic genetic oscillators
    Octavio Mondragón-Palomino
    Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093 0412, USA
    Science 333:1315-9. 2011
    ..Computational simulations indicated that natural oscillators may contain a positive-feedback loop to robustly adapt to environmental cycles...
  73. ncbi Free flow acoustophoresis: microfluidic-based mode of particle and cell separation
    Filip Petersson
    Department of Electrical Measurements, Lund Institute of Technology, Lund, Sweden
    Anal Chem 79:5117-23. 2007
    ..The results show that free flow acoustophoresis can be used to perform complex separation tasks, thereby offering an alternative to expensive and time-consuming methods currently in use...
  74. pmc Three-dimensional microfluidic devices fabricated in layered paper and tape
    Andres W Martinez
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 105:19606-11. 2008
    ..Three-dimensional paper-based microfluidic devices are especially appropriate for use in distributed healthcare in the developing world and in environmental monitoring and water analysis...
  75. pmc Perfused multiwell plate for 3D liver tissue engineering
    Karel Domansky
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    Lab Chip 10:51-8. 2010
    ..After seven days of culture, tissue formed from cells seeded in the perfused multiwell reactor remained functionally viable as assessed by immunostaining for hepatocyte and liver sinusoidal endothelial cell (LSEC) phenotypic markers...
  76. pmc Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of C. elegans
    S Elizabeth Hulme
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St, Cambridge, MA 02138, USA
    Lab Chip 10:589-97. 2010
    ..This ability to perform longitudinal measurements within the device enabled the identification of age-related phenotypic changes that correlate with lifespan in C. elegans...
  77. ncbi Isolation of tumor cells using size and deformation
    Hisham Mohamed
    Wadsworth Center, New York State Department of Health, Albany, NY 12201 0509, USA
    J Chromatogr A 1216:8289-95. 2009
    ..Furthermore, this work will use gene expression profiles to gain insights into metastasis...
  78. ncbi Integrated microfluidic system for rapid screening of CRP aptamers utilizing systematic evolution of ligands by exponential enrichment (SELEX)
    Chao June Huang
    Department of Engineering Science, National Cheng Kung University, 1, University Road, Tainan, Taiwan
    Biosens Bioelectron 25:1761-6. 2010
    ..The developed microsystem demonstrated fast screening of CRP aptamers and can be used as a powerful tool to select analyte-specific aptamers for biomedical applications...
  79. ncbi Automated on-chip rapid microscopy, phenotyping and sorting of C. elegans
    Kwanghun Chung
    School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Dr NW, Atlanta, Georgia 30332, USA
    Nat Methods 5:637-43. 2008
    ..Screening time can be reduced by orders of magnitude; moreover, screening is completely automated...
  80. ncbi Biological applications of microfluidic gradient devices
    Sudong Kim
    School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151 744, Korea
    Integr Biol (Camb) 2:584-603. 2010
    ..The principles and applications of microfluidic gradient generation in both 2D and 3D cellular microenvironments are discussed with emphasis on approaches to manipulate spatial and temporal distribution of signaling molecules...
  81. ncbi Zebrafish embryo development in a microfluidic flow-through system
    Eric M Wielhouwer
    Institute of Biology, Leiden University, Sylvius Laboratory, Sylviusweg 72, 2333, BE, Leiden, The Netherlands
    Lab Chip 11:1815-24. 2011
    ..We discuss how biochip technology, coupled with zebrafish larvae, could allow biological research to be conducted in massive, parallel experiments, at high speed and low cost...
  82. pmc Single-molecule denaturation mapping of DNA in nanofluidic channels
    Walter Reisner
    Department of Physics, McGill University, Montreal, QC, Canada
    Proc Natl Acad Sci U S A 107:13294-9. 2010
    ..This technique may serve as the basis for a new mapping technology ideally suited for investigating the long-range structure of entire genomes extracted from single cells...
  83. pmc Pre-dispositions and epigenetic inheritance in the Escherichia coli lactose operon bistable switch
    Lydia Robert
    INSERM, and Faculty of Medicine, Paris Descartes University, 24 Rue de Faubourg St Jacques, Paris, France
    Mol Syst Biol 6:357. 2010
    ..Thus, stochasticity at the local level of the network and global physiology are synergistically involved in cell response determination...
  84. ncbi Drop-based microfluidic devices for encapsulation of single cells
    Sarah Köster
    Department of Physics and School of Engineering and Applied Sciences, Harvard University, Cambridge, USA
    Lab Chip 8:1110-5. 2008
    ..These devices hold the promise of developing microfluidic cell cytometers and cell sorters with much greater functionality, allowing assays to be performed on individual cells in their own microenvironment prior to analysis and sorting...
  85. pmc Femtosecond laser nanoaxotomy lab-on-a-chip for in vivo nerve regeneration studies
    Samuel X Guo
    Department of Mechanical Engineering, The University of Texas at Austin, 204 E Dean Keeton Street, Austin, Texas 78712, USA
    Nat Methods 5:531-3. 2008
    ..Using the 'nanoaxotomy' chip, we discovered that axonal regeneration occurs much faster than previously described, and notably, the distal fragment of the severed axon regrows in the absence of anesthetics...
  86. ncbi A hydrogel-based microfluidic device for the studies of directed cell migration
    Shing Yi Cheng
    School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA
    Lab Chip 7:763-9. 2007
    ..This device will also be useful in controlling the chemical and mechanical environment during the formation of tissue engineered constructs...
  87. pmc Biomolecular gradients in cell culture systems
    Thomas M Keenan
    Department of Bioengineering, University of Washington, Washington 98195, USA
    Lab Chip 8:34-57. 2008
    ..For each method, we outline the salient features, capabilities, and applications...
  88. pmc Continuous inertial focusing, ordering, and separation of particles in microchannels
    Dino Di Carlo
    BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Shriners Hospital for Children, and Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 104:18892-7. 2007
    ....
  89. ncbi Microfabricated platform for studying stem cell fates
    Vicki I Chin
    Department of Bioengineering, 9500 Gilman Dr MC, University of California, San Diego, La Jolla, California 92093 0412, USA
    Biotechnol Bioeng 88:399-415. 2004
    ....
  90. ncbi Microfluidic culture platform for neuroscience research
    Jeong Won Park
    Department of Biomedical Engineering, University of California, Irvine, 3410 Natural Science II, Irvine, California 92697, USA
    Nat Protoc 1:2128-36. 2006
    ..The microfluidic device provides a highly adaptable platform for neuroscience research and may find applications in modeling CNS injury and neurodegeneration. This protocol can be completed in 1-2 days...
  91. ncbi Sorting cells by size, shape and deformability
    Jason P Beech
    Division of Solid State Physics, Lund University, Lund, Sweden
    Lab Chip 12:1048-51. 2012
    ....
  92. ncbi Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform
    Kameel Abi-Samra
    Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA
    Lab Chip 11:723-6. 2011
    ..Overall, the techniques presented in this report offer novel methods for liquid handling, separation, and storage on the centrifugal microfluidic disc platform...
  93. ncbi Engineered approaches to the stem cell microenvironment for cardiac tissue regeneration
    Ebrahim Ghafar-Zadeh
    Department of Bioengineering, University of California, Berkeley, USA
    Lab Chip 11:3031-48. 2011
    ....
  94. ncbi Digital microfluidics-enabled single-molecule detection by printing and sealing single magnetic beads in femtoliter droplets
    Daan Witters
    KU Leuven University of Leuven, BIOSYST MeBioS, Willem de Croylaan 42, Leuven, Belgium
    Lab Chip 13:2047-54. 2013
    ....
  95. ncbi Bacteria concentration using a membrane type insulator-based dielectrophoresis in a plastic chip
    Yoon Kyoung Cho
    School of Nano Biotechnology and Chemical Engineering, UNIST, Ulsan, Republic of Korea
    Electrophoresis 30:3153-9. 2009
    ..The maximal trapping efficiency of 66+/-7% was obtained under the electric fields (E=128 V/mm and f=300 kHz) when the dilute bacteria solution (Escherichia coli: 9.3 x 10(3) cell/mL, 0.5 mS/m) flowed with a flow rate of 100 microL/min...
  96. ncbi Biological cells on microchips: new technologies and applications
    Yo Tanaka
    Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Biosens Bioelectron 23:449-58. 2007
    ..Understanding fundamental concepts behind the distinct features and performance characteristics of these cell-based micro-systems will lead to development of new devices that will be exploited in various fields in the future...
  97. ncbi Neuroscience goes on a chip
    Aung K Soe
    Centre for Intelligent Systems Research, Deakin University, Australia
    Biosens Bioelectron 35:1-13. 2012
    ..Next, we present LOC counterparts for those bench-top instrumentations. Finally, we offer perspectives on persistent challenges and our perception of opportunities based on LOC instrumentations in neuroscience research...
  98. ncbi MITOMI: a microfluidic platform for in vitro characterization of transcription factor-DNA interaction
    Sylvie Rockel
    Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
    Methods Mol Biol 786:97-114. 2012
    ..MITOMI allows the detection of transient and low-affinity TF-DNA interactions in high-throughput...
  99. pmc Continuous analysis of dye-loaded, single cells on a microfluidic chip
    K Scott Phillips
    Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA
    Lab Chip 11:1333-41. 2011
    ..Automated cell detection and lysis were required to reduce peak FWHM variability to 30% RSD. A maximum throughput of 30 cells min(-1) was achieved. Device stability was demonstrated by analyzing 600 single cells over a 2 h time span...
  100. pmc Acoustophoretic synchronization of mammalian cells in microchannels
    Patrick Thévoz
    Department of Mechanical Engineering, University of California, Santa Barbara, California 93106, USA
    Anal Chem 82:3094-8. 2010
    ..We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G(1) phase synchrony (approximately 84%) and throughput (3 x 10(6) cells/h per microchannel)...
  101. pmc Chemotaxis of cell populations through confined spaces at single-cell resolution
    Ziqiu Tong
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 7:e29211. 2012
    ..Our novel microfluidic design is therefore a powerful tool for the study of cellular chemotaxis, and is suitable for a wide range of biological and biomedical applications...

Research Grants82

  1. Personalized Clinical Diagnostics and Beyond: Integrated Ring Resonator Arrays
    Ryan Bailey; Fiscal Year: 2009
    ..This technology promises to broadly impact the landscape of the biomedical sciences, both meeting the clinical diagnostic challenges of today and pioneering the paradigm-shifting discoveries of tomorrow. ..
  2. Re-Wiring Cellular Metabolism to Control Biofilm Formation and Virulence BY...
    Thomas K Wood; Fiscal Year: 2011
    ..If virulence genes can also be controlled in biofilms, then novel treatments can be envisioned for the 80% of bacterial infections that occur in biofilms where antibiotics are often ineffective. ..
  3. Multiplexed Detection of Food and Waterborne Pathogens
    Francis Barany; Fiscal Year: 2011
    ..The PCR/LDR assays will be migrated to the Cepheid GeneXpert system as well as other microfluidic devices initially using capillary electrophoresis or LDR-FRET, and subsequently Universal Array readout. ..
  4. Negative regulation of virulence in Pseudomonas aeruginosa
    Albert Siryaporn; Fiscal Year: 2013
    ..The findings from this study will shed light on how bacterial cells detect surfaces and how virulence is regulated. Additionally, it may also provide further details about the initial stages of biofilm formation. ..
  5. Grafting DNA Codes on Paper for Rapid, Low-Cost, and Sensitive Meningitis Diagnos
    Xiujun Li; Fiscal Year: 2013
    ..It will also have great potential in the detection of various plant, animal, foodborne, and infectious diseases from other microorganisms. ..
  6. Automated Microscope for Long-Duration, Quantitative Dynamic Imaging
    JOHN PETER WIKSWO; Fiscal Year: 2010
    ....
  7. Controlled Chemotaxis and Differentiation of Embryonic Cardiac Progenitor Cells
    Gregory M Fomovsky; Fiscal Year: 2013
    ..The proposed research uses rigorous approaches to investigate these mechanisms in cardiac progenitor cells, and may lead to the design of successful cell therapies. ..
  8. Neurovascular Unit on a Chip: Chemical Communication, Drug and Toxin Responses
    JOHN PETER WIKSWO; Fiscal Year: 2013
    ..An integrated approach will provide technologies of widespread applicability and reveal new mechanistic and region-specific insights into how the brain receives, modifies, and is affected by drugs, neurotropic agents and disease. ..
  9. Microchip to Detect Influenza Infection and Type in Nasopharyngeal Swabs
    Catherine M Klapperich; Fiscal Year: 2010
    ....
  10. Nrf2 as a critical determinant of smooth muscle function
    Steven S An; Fiscal Year: 2013
    ..Here we focus on the structure and function of airway smooth muscle - the end organ that leads to airway constriction - and the role for a transcription factor (Nrf2) in the context of airway wall remodeling in chronic asthma. ..
  11. Fast, High-Resolution Protein Separations using Controllable Electro-Osmotic Flow
    FRANK JAHNKE; Fiscal Year: 2010
    ....
  12. Dopamine action in pancreatic islet function
    David W Piston; Fiscal Year: 2013
    ....
  13. Molecular and Functional Characterization of Colon Tumor Cancer Stem Cells and St
    Michael Clarke; Fiscal Year: 2010
    ..abstract_text> ..
  14. Microfluidic devices for studying genetic competence in Streptococcus mutans
    STEPHEN JAMES HAGEN; Fiscal Year: 2010
    ..The goal of this project is to develop new laboratory devices with which we can study, and perhaps learn to control, this behavior. ..
  15. Probing the real-time kinetics and steady-state dynamics of gene expression
    Narendra Maheshri; Fiscal Year: 2013
    ..Together, these aims will help establish simple ways of incorporating crucial dynamical properties in models and yield mechanistic insights by comparing how different features of promoters and activators affect kinetics. ..
  16. Microfluidic Processing of Leukocytes for Molecular Diagnostic Testing
    James C Sturm; Fiscal Year: 2012
    ..Applications include research and clinical diagnostics in cancer, infectious disease, and inflammatory disease, among other disease areas. ..
  17. Microfluidic devices for determining dynamics of islets of Langerhans
    MICHAEL GABRIEL ROPER; Fiscal Year: 2013
    ..Ultimately, such knowledge has the potential to guide the development of therapeutics for reducing the problems associated with glucose toxicity in type II diabetics. ..
  18. Machine Vision Analysis of C. Elegans Phenotypic Patterns
    Weiwei Zhong; Fiscal Year: 2013
    ..imported from WormBase. Software and protocols for hardware construction will be freely available from laboratory websites. ..
  19. Dissecting the mechanism of direct neural induction through genomic interrogation
    MARIUS contact WERNIG; Fiscal Year: 2010
    ..Patient-derived neurons could be used for modeling neurological diseases or as cell grafts to treat neurodegenerative diseases like Parkinson's disease. ..
  20. Disposable Microfluidic Devices for Point of Care Diagnostics
    Catherine M Klapperich; Fiscal Year: 2010
    ..difficile, we envision building devices (in 6-8 years) that could distinguish between different infectious diarrheas and guide treatment in settings where antibiotic supplies are limited. ..
  21. An integrated platform for blood collection, HIV viral RNA purification, and stab
    Erwin Berthier; Fiscal Year: 2013
    ..Additionall, a nucleic acid amplification / quantitation component will be developed, enabling complete sample-to-answer operation and further mitigating barriers to HIV viral load testing access. ..
  22. A Microfluidic Device for Label-free HIV Virion Count at Point of Care Settings
    Xuanhong Cheng; Fiscal Year: 2010
    ..The current proposal intends to address the challenges by developing a label-free, HIV whole particle counter that can be used at the point of care and in resource limited settings. ..
  23. Integrated microfluidic devices for preterm birth biomarker measurement
    ADAM THOMAS WOOLLEY; Fiscal Year: 2013
    ....
  24. Sensitive and multiplexed analysis of cancer biomarkers with QD barcodes
    Xiaohu Gao; Fiscal Year: 2012
    ..We will further carry out experiments to detect mutations on both DNA and RNA level as well as the profiles of protein cancer biomarkers, which can be isolated from serum or homogenized cell and tissue specimens. ..
  25. Novel Surfaces for Enriching Epithelial Cells: A Breastmilk Model
    Maria M Santore; Fiscal Year: 2013
    ....
  26. Host cell receptor variation and control of viral cross-species transmission
    ANDREW BROWNELL ALLISON; Fiscal Year: 2013
    ..These studies will allow us to uniquely determine the interplay between the viral capsid and host receptors that mediate cross-species transmission and emergence. ..
  27. Uncovering microbial modulators of neuroactive molecules as novel therapeutics.
    Elaine Hsiao; Fiscal Year: 2013
    ..I will also begin mentoring up to two biology or chemistry undergraduate students per year through Caltech's summer research program. ..
  28. Point Detection of Pathogens in Oral Samples via UPT
    Daniel Malamud; Fiscal Year: 2010
    ..pyogenes in oral samples, the platform can easily be converted to look at other types of samples (blood, nasopharyngeal and oral swabs, urine), and other pathogens including emerging infectious agents such as avian H5N1 virus. ..
  29. Nanofluidic Devices for Studying Assembly of Single Virus Particles
    Stephen C Jacobson; Fiscal Year: 2013
    ....
  30. Stem Cell Behavior in Complex Microfluidic Gradients
    SUSAN RENEE OPALENIK; Fiscal Year: 2010
    ..It is anticipated that data obtained from the proposed experiments will be useful in designing therapeutic modalities to enhance wound repair, decrease scarring, and produce a better clinical outcome. ..
  31. Microfluidic devices for high-throughput anthelmintic screens
    Shawn R Lockery; Fiscal Year: 2010
    ..This goal of project is to reduce the cost of finding new anti- nematode drugs by developing a new screening process that combines microtechnology and electrophysiology for the first time. ..
  32. Quantitative studies of cell cycle checkpoints and switches
    DAVID OWEN MORGAN; Fiscal Year: 2013
    ....
  33. Cell-based sensors for measuring impact of microsystems on cell physiology
    Joel Voldman; Fiscal Year: 2013
    ..We are proposing to develop cell "sensors" that would glow colors if they are subjected to stresses from microsystems. ..
  34. An open microfluidic device to study chemotaxis of eukaryotic cells
    Dawit Jowhar; Fiscal Year: 2012
    ..Training and implementation on the use of appropriate equipment and techniques will be facilitated by qualified personnel in the Janetopoulos laboratory and the staff at Vanderbilt University medical center. ..
  35. High Throughput 3D Cell Assay for Metastatic Prostate Cancer
    Shuichi Takayama; Fiscal Year: 2013
    ..The biological hypothesis that motivates this study is: We can treat metastatic PCa better by developing drugs that specifically target dormant metastatic PCa cells that are parasitizing the HSC niche. PHS 398 (Rev. 11/07) Page 1 ..
  36. Quantitative Analysis of Cancer Cell Motility using Microfluidic Devices
    Daniel Irimia; Fiscal Year: 2011
    ..This could prevent metastases before they occur, transforming cancer into a chronic disease with which the patient can live a longer period of time. ..
  37. Development of a Vascularized In-Vitro Model of the Tumor Microenvironment
    Thomas Neumann; Fiscal Year: 2010
    ..We expect our model to become a valuable system in cancer research, commercially available to scientists in academia and industry. ..
  38. Nanoscale Optofluidic Pathogen Detection
    David Erickson; Fiscal Year: 2009
    ..The near term major application will be as an early stage detection platform. ..
  39. Regulation of growth cone guidance by localized microRNA
    Gary J Bassell; Fiscal Year: 2013
    ..These studies have important implications for future therapeutic strategies to modulate microRNA expression or function to manipulate axonal growth and connectivity in the treatment of neurologic disorders. ..
  40. NEURONAL AND THEORETICAL ANALYSIS OF SPATIAL ORIENTATION
    Shawn R Lockery; Fiscal Year: 2013
    ..The research is therefore likely to help trace causal connections from genetic differences to mental disorders. ..
  41. Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development
    JOSE MANUEL MORACHIS; Fiscal Year: 2010
    ..This new fully developed cytometer will serve demands for decentralized, inexpensive tools for cutting edge research applications in diverse fields including cancer, stem cell biology, immunology, pathology, and epigenetics. ..
  42. Visualization of shear stress-induced endothelial cell signaling.
    Eugene Tkachenko; Fiscal Year: 2009
    ..These exploratory studies have the potential to enable the facile analysis of flow-mediated signaling in living endothelial cells. ..
  43. Imaging synapse formation using novel microfluidic platforms
    Donna J Webb; Fiscal Year: 2012
    ..The development of these microfluidic platforms will be of great interest and benefit to neurobiologist by providing a platform for identifying the key molecular signals that regulate the assembly of synapses. ..
  44. Integrated Platform for In Vitro Culturing of Cells and Tissues
    Colleen K Van Pelt; Fiscal Year: 2013
    ..In summary, the interconnect platform will assist in the commercialization of "cells-on-a- chip" applications, enabling scientifc breakthroughs and greater understanding of complex biological systems. ..
  45. Simulation Tool to Rapidly Design Optimize and Prototype Microfluidic Devices
    Yi Wang; Fiscal Year: 2010
    ..Target commercial applications for the developed technology include several established and emerging market sectors including pharmaceutical, biotechnology, clinical diagnostics, medical devices, and drug delivery. ..
  46. Microfluidic devices for investigation of rare T cell populations
    Joung Hyun Lee; Fiscal Year: 2013
    ..This platform also has wide application in other cellular systems, including investigation of cancer and stem cell physiologies. ..
  47. Rapid Phenotyping and Visual Screens Enabled by microfluidics
    IVAN DE CARLOS CACERES; Fiscal Year: 2012
    ..In general, understanding the mechanisms behind behavior in any organism will help to further explain the mysteries of the human brain and potentially aid in the discovery of therapeutic treatments for various diseases. ..
  48. Microfluidic Cell Separation for Tissue Engineering and Regenerative Medicine
    SHASHI MURTHY; Fiscal Year: 2012
    ..A key component of either of these approaches is the requirement to isolate or harvest certain cell types prior to utilizing them for repair and regenerative applications and this proposal aims to create novel tools for this purpose. ..
  49. Understanding cell migration through microscale in vitro models
    Anna Huttenlocher; Fiscal Year: 2013
    ..Future applications will include analysis of neutrophil motility from patients presenting with neutropenia using 2D and 3D/cell sourced microfluidic systems to understand disease pathogenesis and identify novel therapeutic targets. ..
  50. Microfluidic Devices for Molecular Characterization of Cancer Cells at the Single
    John Zhong; Fiscal Year: 2010
    ..After developing and optimizing the device with this R21 proposal, a R33 proposal will be submitted to apply this device for molecular characterization of CTC captured from bloods of cancer patients at the single-cell level. ..
  51. Self-powered Integrated Microfluidic Blood Analysis System (SIMBAS) for Molecular
    IVAN DIMOV; Fiscal Year: 2012
    ..Proof-of-concept detection of tuberculosis (TB) and human immunodeficiency virus (HIV) will be demonstrated. ..
  52. Microchips of Protein Kinase Substrates
    XIAOCHUAN contact ZHOU; Fiscal Year: 2010
    ....
  53. Microfluidic Platform for Preparation of Biomolecule Based Nuclear Imaging Probes
    David E Reichert; Fiscal Year: 2013
    ..4. With the successful development of the individual devices, develop a single integrated device incorporating the optimized versions of the individual devices of the first aim. ..
  54. Systems Biology of Cell Decision Processes
    PETER KARL SORGER; Fiscal Year: 2012
    ..abstract_text> ..
  55. Blood rheology: analytical approaches to sickle cell disease
    Lance Munn; Fiscal Year: 2009
    ..When completed, this study will provide a formal framework for understanding and overcoming rheological aberrations in sickle cell disease. ..
  56. VEC3-Valve Enabled Cell Co-Culture Platforms for Cancer Biology Study
    Donna J Webb; Fiscal Year: 2012
    ..The overall quantitative milestone is to achieve 95% success rate in assays using the VEC3 platforms for tumor angiogenesis, intravsation and metastasis studies with quantitative parameters of cellular communication. ..
  57. Development of 3D Micro-scale Engineered Tissue Model Systems for Drug Discovery
    Wei Li; Fiscal Year: 2009
    ..The engineered tissue model systems proposed in this research will have wide applications in drug discovery and development, as well as in basic and clinical research. ..
  58. Experimental and mathematical analysis of delay in transcripitional signaling
    Matthew R Bennett; Fiscal Year: 2013
    ....
  59. A Universal Front End to Improve Assembly Outcomes for Next-Gen Sequencing and Re
    ERIC S SHAQFEH; Fiscal Year: 2010
    ....
  60. Development of Nanoscale Plasmonic Devices for Creation of a Next Generation Surf
    David H Waldeck; Fiscal Year: 2012
    ....
  61. Microfluidics for malaria pathogenesis in Africa
    Pradipsinh K Rathod; Fiscal Year: 2010
    ....
  62. Rapid Generation of High Affinity Protein Sensor Elements
    HYONGSOK SOH; Fiscal Year: 2009
    ..If successful, the proposed MVX technology has the potential to fundamentally revolutionize the way we generate affinity ligands, and perform viral diagnostics. ..
  63. Use of Bioconjugated Quantum Dots in Micro FlowCytometer
    Joel Tabb; Fiscal Year: 2005
    ..In the Phase II, we will develop and construct a micro flow cytometer system specifically designed for use with quantum dot conjugated probes and then develop a number of practical, high utility assays. ..
  64. Novel Fluid Management System for Microfluidic Devices
    ALAN CISAR; Fiscal Year: 2009
    ..Cost effective next generation health care products and diagnostics will allow physicians to provide improved and more individualized patient treatment and care. ..
  65. High Throughput Rare Cell Isolation System
    CAROLYN CONANT; Fiscal Year: 2009
    ..This proposal aims to develop a high throughput system to isolate rare cells from biological samples and provide a range of analysis options to accommodate the desired endpoints of both clinical and research applications. ..
  66. Cell Graft Engineering Using Microfluids
    Allison Hubel; Fiscal Year: 2007
    ..Reducing cell losses during processing will also increase the access of stem cell based therapies (in particular cord blood stem cells) to more people. [unreadable] [unreadable]..
  67. A microfluidic platform to study the effects of chemokine gradients on prostate c
    Jung Chih Chiao; Fiscal Year: 2009
    ....
  68. DNA sequencing by ELFSE on microfluidic devices
    Annelise Barron; Fiscal Year: 2006
    ..Theoretical predictions will guide every aspect of ELFSE experimentation. [unreadable] [unreadable]..
  69. Novel MEMS and Microfluidic Platforms for Drug Delivery
    RONALD SIEGEL; Fiscal Year: 2004
    ..3. In the third project, a simple microfluidic device, which is expected to produce extremely uniform hydrogel microspheres, will be constructed and tested. ..
  70. Lensfree On-Chip Near-field Microscopy based on Resonant Nano-Apertures
    Aydogan Ozcan; Fiscal Year: 2009
    ....
  71. Integrated Microfluidic Devices for 18F Labeled PET Probes in Cancer Imaging
    Hsian Rong Tseng; Fiscal Year: 2010
    ..The long term objective of this proposal is to make [18F]-labeled PET probes easily accessible so that researchers and clinicians can perform a wide range of PET studies targeting specific molecular lesions in cancer. ..
  72. 2007 Microfluidics, Physics & Chemistry of Gordon Research Conference
    SABETH VERPOORTE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  73. Lab-on-a-chip for multiplexed newborn screening of lysosomal storage disorders
    Vamsee Pamula; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  74. A PROGRAMMABLE LIGHT SOURCE FOR MICROFLUIDIC DEVICES
    MARKUS HEHLEN; Fiscal Year: 1999
    ..PROPOSED COMMERCIAL APPLICATION: The proposed commercial application is a compact, programmable light source for microfluidic devices/instruments. ..
  75. Microfluidic platforms for severe malaria
    PRADIPSINH RATHOD; Fiscal Year: 2007
    ..In the future, the devices developed here may also find utility in studying adhesion and natural immunity in malaria, and in control of other infectious diseases. [unreadable] [unreadable]..
  76. Materials and Strategies for Lab-on-a-Chip
    Sonia Grego; Fiscal Year: 2007
    ..Advances in fabrication techniques, such as nanoimprint lithography, for micro- and nanofluidic applications [unreadable] On-chip detection approaches [unreadable] World-to-chip interface [unreadable] [unreadable] [unreadable]..
  77. Photoablation System to Make Microfluidic Structures
    KANTI JAIN; Fiscal Year: 2003
    ..The applications of these constructions are far-ranging, including medical diagnostics, chemical detection and fabrication, and water quality control. ..
  78. Use of Bioconjugated Quantum Dots in Micro FlowCytometer
    Joel Tabb; Fiscal Year: 2003
    ..Once these quantum dots are bioconjugated, they will be tested in a microfluidic flow cytometer developed by Agave BioSystems to demonstrate their capabilities for the detection and quantification of fluorescently labeled cells. ..
  79. University of Chicago Biomedical Cleanroom
    Michael D Hopkins; Fiscal Year: 2010
    ..The user group includes investigators from departments in the biological and physical science divisions as well as researchers from the University's medical complex. ..
  80. Microbead INtegrated DNA Sequencer (MINDS) System
    Stevan Jovanovich; Fiscal Year: 2007
    ..Future work will explore the development of lower-throughput versions appropriate for core and individual laboratories. [unreadable] [unreadable]..
  81. Nucleic Acid Binding & Amplifications Matrices
    JEFFREY IVES; Fiscal Year: 2002
    ..A list of the most prominent would include health care, biowarfare defense, food and water safety, environmental monitoring, screening for genetic predispositions, tissue typing, forensics, and parentage verification. ..
  82. A Portable Biosensor for Francisella tularensis
    CLARK EDSON; Fiscal Year: 2003
    ..tularensis. ..