immunodiffusion

Summary

Summary: Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.

Top Publications

  1. ncbi Seroprevalence of bovine leukemia virus in dairy cattle in Argentina: comparison of sensitivity and specificity of different detection methods
    K G Trono
    , C.I.C.V.yA. INTA Castelar, , Pvcia, Buenos Aires, Argentina
    Vet Microbiol 83:235-48. 2001
  2. ncbi Serum lactoferrin concentrations in colostrum-fed calves
    Nicole M Holloway
    Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA
    Am J Vet Res 63:476-8. 2002
  3. ncbi Relative sensitivity and specificity of agar gel immunodiffusion, enzyme immunosorbent assay, and immunoblotting for detection of anti-bovine leukemia virus antibodies in cattle
    K Yeon Choi
    Department of Microbiology Immunology, University of California, Davis, CA, USA
    J Virol Methods 104:33-9. 2002
  4. ncbi Agar gel immunodiffusion test (AGID) evaluation for detection of bovine paratuberculosis in Rio de Janeiro, Brazil
    R Ferreira
    Department of Medical Microbiology, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    Lett Appl Microbiol 35:173-5. 2002
  5. pmc Diagnostic testing patterns of natural Mycobacterium paratuberculosis infection in pygmy goats
    Elizabeth J B Manning
    University of Wisconsin Madison, School of Veterinary Medicine, 2015 Linden Drive, Madison, Wisconsin 53706, USA
    Can J Vet Res 67:213-8. 2003
  6. ncbi Avian glycan-specific IgM monoclonal antibodies for the detection and quantitation of type A and B haemagglutinins in egg-derived influenza vaccines
    Isabelle Legastelois
    Department of Research, Sanofi Pasteur, 1541 Avenue Marcel Merieux, 69280 Marcy l Etoile, France
    J Virol Methods 178:129-36. 2011
  7. ncbi Development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion
    Jesse Bodle
    CSL Ltd, Parkville, Vic, Australia
    Influenza Other Respir Viruses 7:191-200. 2013
  8. ncbi Simplified method for producing Paracoccidioides brasiliensis exoantigens for use in immunodiffusion tests
    Z P Camargo
    Disciplina de Biologia Celular, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Sao Paulo, Brasil
    Med Mycol 41:539-42. 2003
  9. pmc Evaluation of five enzyme-linked immunosorbent assays and an agar gel immunodiffusion test for detection of antibodies to small ruminant lentiviruses
    J Brinkhof
    AHS Deventer, P O Box 9, 7400 AA Deventer, The Netherlands
    Clin Vaccine Immunol 14:1210-4. 2007
  10. ncbi A simple slot blot for the detection of virtually all subtypes of the influenza A viral hemagglutinins using universal antibodies targeting the fusion peptide
    Changgui Li
    National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, P R China
    Nat Protoc 5:14-9. 2010

Research Grants

Detail Information

Publications245 found, 100 shown here

  1. ncbi Seroprevalence of bovine leukemia virus in dairy cattle in Argentina: comparison of sensitivity and specificity of different detection methods
    K G Trono
    , C.I.C.V.yA. INTA Castelar, , Pvcia, Buenos Aires, Argentina
    Vet Microbiol 83:235-48. 2001
    ..The agar gel immunodiffusion (AGID) test has been the serological test of choice for routine diagnosis of serum samples...
  2. ncbi Serum lactoferrin concentrations in colostrum-fed calves
    Nicole M Holloway
    Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA
    Am J Vet Res 63:476-8. 2002
    ..To determine serum lactoferrin concentrations (SLFC) in neonatal calves before and after ingestion of colostrum and to develop models that predict SLFC as a function of colostral lactoferrin concentrations (CLFC) in calves...
  3. ncbi Relative sensitivity and specificity of agar gel immunodiffusion, enzyme immunosorbent assay, and immunoblotting for detection of anti-bovine leukemia virus antibodies in cattle
    K Yeon Choi
    Department of Microbiology Immunology, University of California, Davis, CA, USA
    J Virol Methods 104:33-9. 2002
    ..A widely used commercial agarose immunodiffusion screening assay and a commercial antibody capture enzyme immunosorbent assay were compared for sensitivity ..
  4. ncbi Agar gel immunodiffusion test (AGID) evaluation for detection of bovine paratuberculosis in Rio de Janeiro, Brazil
    R Ferreira
    Department of Medical Microbiology, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    Lett Appl Microbiol 35:173-5. 2002
    ..The aim of this study is to evaluate the AGID serological test for detection of antibodies anti-Mycobacterium paratuberculosis and its possible adoption as diagnostic method in our field conditions...
  5. pmc Diagnostic testing patterns of natural Mycobacterium paratuberculosis infection in pygmy goats
    Elizabeth J B Manning
    University of Wisconsin Madison, School of Veterinary Medicine, 2015 Linden Drive, Madison, Wisconsin 53706, USA
    Can J Vet Res 67:213-8. 2003
    ..The diverse antemortem testing patterns seen in these goats underscore the importance of using varied diagnostic assays serially or in parallel to increase the likelihood of identifying all infected goats...
  6. ncbi Avian glycan-specific IgM monoclonal antibodies for the detection and quantitation of type A and B haemagglutinins in egg-derived influenza vaccines
    Isabelle Legastelois
    Department of Research, Sanofi Pasteur, 1541 Avenue Marcel Merieux, 69280 Marcy l Etoile, France
    J Virol Methods 178:129-36. 2011
    ..9μg/ml, as opposed to the 5μg/ml quantitation limit generally accepted for the standard single-radial-immunodiffusion (SRID) assay, the approved technique for quantifying HA content in influenza vaccines...
  7. ncbi Development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion
    Jesse Bodle
    CSL Ltd, Parkville, Vic, Australia
    Influenza Other Respir Viruses 7:191-200. 2013
    ..The current method used to measure haemagglutinin (HA) content for influenza vaccine formulation, single radial immunodiffusion (SRID), is lengthy and relies on the availability of matched standardised homologous reagents...
  8. ncbi Simplified method for producing Paracoccidioides brasiliensis exoantigens for use in immunodiffusion tests
    Z P Camargo
    Disciplina de Biologia Celular, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Sao Paulo, Brasil
    Med Mycol 41:539-42. 2003
    A simplified method to produce Paracoccidioides brasiliensis exoantigens for immunodiffusion testing is proposed...
  9. pmc Evaluation of five enzyme-linked immunosorbent assays and an agar gel immunodiffusion test for detection of antibodies to small ruminant lentiviruses
    J Brinkhof
    AHS Deventer, P O Box 9, 7400 AA Deventer, The Netherlands
    Clin Vaccine Immunol 14:1210-4. 2007
    ..To improve the quality of testing, five enzyme-linked immunosorbent assays (ELISAs) and an agar gel immunodiffusion test (AGIDT) were evaluated...
  10. ncbi A simple slot blot for the detection of virtually all subtypes of the influenza A viral hemagglutinins using universal antibodies targeting the fusion peptide
    Changgui Li
    National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, P R China
    Nat Protoc 5:14-9. 2010
    ..The entire procedure takes only 5 h and can be applied to the quantitative determination of virtually all influenza viral HAs using a single antibody targeting the fusion peptide...
  11. ncbi Physicochemical studies on the stability of influenza haemagglutinin in vaccine bulk material
    Dion M A M Luykx
    Center for Biological Medicines and Medical Technology, National Institute for Public Health and the Environment RIVM, P O Box 1, 3720 BA Bilthoven, The Netherlands
    Eur J Pharm Sci 23:65-75. 2004
    ..In conclusion, fluorescence and circular dichroism spectroscopy are highly suitable techniques to monitor the stability of haemagglutinin in a straightforward and fast way...
  12. pmc Evaluation of agar gel immunodiffusion serology using caprine and ovine lentiviral antigens for detection of antibody to caprine arthritis-encephalitis virus
    D P Knowles
    Agricultural Research Service, U S Department of Agriculture, Pullman, Washington 99164 7030
    J Clin Microbiol 32:243-5. 1994
    The sensitivity of the agar gel immunodiffusion (AGID) test for the detection of antibody to caprine arthritis-encephalitis virus (CAEV) was investigated with CAEV or ovine progressive pneumonia virus (OPPV) as the source of antigen...
  13. ncbi Detection of horses infected naturally with equine infectious anemia virus by nested polymerase chain reaction
    M M Nagarajan
    Retrovirology Centre of Expertise, Canadian Food Inspection Agency (CFIA, 93 Mount Edward Road, PEI, C1A 5T1, Charlottetown, Canada
    J Virol Methods 94:97-109. 2001
    ..developed in the present study detected more EIAV positive animals and was found as specific as the agar gel immunodiffusion (Coggins) assay and offers great potential a diagnostic test for the detection of EIAV infections in field ..
  14. pmc Detection of equine infectious anemia viral RNA in plasma samples from recently infected and long-term inapparent carrier animals by PCR
    J L Langemeier
    Maxwell Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington 40546 0099, USA
    J Clin Microbiol 34:1481-7. 1996
    ..The RT-nPCR assay, agar gel immunodiffusion test, and conventional virus isolation were compared for detection of early infection in 12 experimentally ..
  15. ncbi The porcine acute phase protein response to acute clinical and subclinical experimental infection with Streptococcus suis
    N S Sorensen
    Department of Veterinary Diagnostics and Research, Danish Institute for Food and Veterinary Research, Bulowsvej 27, DK 1790 Copenhagen V, Denmark
    Vet Immunol Immunopathol 113:157-68. 2006
    ..suis infection during a prolonged time period. A diagnostic tool based on such APP-measurements could considerably improve strategic control procedures for this important infection...
  16. ncbi Kinetics of {alpha}2-macroglobulin and {alpha}1-acid glycoprotein in rats subjected to repeated acute inflammatory stimulation
    Toshio Honjo
    Azabu University, Fuchinobe, Sagamihara, Kanagawa, Japan
    Lab Anim 44:150-4. 2010
    ..05 and 0.4 mL/rat injection groups. Differences in peak serum levels in the 0.05 and 0.4 mL/rat groups were attributed to differences in the production of IL-6 and CINC-1, which are thought to contribute to alpha2M and AAG production...
  17. ncbi Evaluation of a Pourquier ELISA kit in relation to agar gel immunodiffusion (AGID) test for assessment of the humoral immune response in sheep and goats with and without Mycobacterium paratuberculosis infection
    S Gumber
    Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden 2570, NSW, Australia
    Vet Microbiol 115:91-101. 2006
    ..0% were observed for positive and negative controls included on 193 plates over a 10-month period and there was a high level of intraassay repeatability with 12.0% of the duplicate samples having CV of >15.0%...
  18. pmc Agar gel immunodiffusion test for the detection of bovine leukemia virus antibodies: lack of trans-Atlantic standardization
    C Simard
    Retrovirology Centre of Expertise, Canadian Food Inspection Agency, Charlottetown, Prince Edward Island
    Can J Vet Res 64:96-100. 2000
    Two agar gel immunodiffusion (AGID) kits for the serodiagnosis of bovine leukemia virus (BLV) were imported from Europe and were compared with North American kits...
  19. pmc Negative immunodiffusion test results obtained with sera of paracoccidioidomycosis patients may be related to low-avidity immunoglobulin G2 antibodies directed against carbohydrate epitopes
    Andreia R Neves
    Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas UNICAMP, Campinas, Brazil
    Clin Diagn Lab Immunol 10:802-7. 2003
    b>Immunodiffusion (ID) is the serologic test most frequently used for the diagnosis and posttherapy follow-up of patients with paracoccidioidomycosis (PCM)...
  20. ncbi Serologic detection of bluetongue virus infection of black-tailed deer: comparison of serum neutralization, agar gel immunodiffusion, and competitive ELISA assays
    J F Patton
    Department of Veterinary Pathology, School of Veterinary Medicine, University of California, Davis 95616
    J Wildl Dis 30:99-102. 1994
    ..irregular intervals thereafter and the presence of virus-specific antibodies in serum determined by agar gel immunodiffusion (AGID), serum neutralization (SN) and competitive enzyme-linked immunosorbent assay (C-ELISA) tests...
  21. ncbi Comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and PCR for the diagnosis of canine brucellosis
    Lara B Keid
    Departamento de Medicina Veterinaria Preventiva e Saude Animal, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo USP, Avenue Prof Dr Orlando Marques Paiva 87, 05508 900 Sao Paulo, SP, Brazil
    Res Vet Sci 86:22-6. 2009
    ..of the rapid slide agglutination test, with and without 2-mercaptoethanol (RSAT and 2ME-RSAT) and agar gel immunodiffusion test (AGID) was evaluated for the diagnosis of brucellosis in naturally infected dogs...
  22. ncbi Comparison of a new gold-immunochromatographic assay for the detection of antibodies against avian influenza virus with hemagglutination inhibition and agar gel immunodiffusion assays
    Dapeng Peng
    College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
    Vet Immunol Immunopathol 117:17-25. 2007
    ..Compared with the "gold standard", i.e. hemagglutination inhibition (HI) and agar gel immunodiffusion (AGID) assays, the gold-immunochromatographic test strip has many advantages, such as high specificity, high ..
  23. ncbi Sensitivity and specificity of the agar-gel-immunodiffusion test, ELISA and the skin test for detection of paratuberculosis in United States Midwest sheep populations
    Suelee Robbe-Austerman
    National Animal Disease Center, Agricultural Research Service, USDA, 2300 Dayton, Ames, Iowa 50010, USA
    Vet Res 37:553-64. 2006
    Our objective was to estimate the sensitivity and specificity of the agar-gel-immunodiffusion test (AGID), the ELISA, and the skin test for the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in sheep using Bayesian ..
  24. ncbi Isolation of Paracoccidioides brasiliensis from armadillos (Dasypus novemcinctus) in an area where the fungus was recently isolated from soil
    M L Silva-Vergara
    Faculdade de Medicina do Triangulo Mineiro, Uberaba, Brazil
    Med Mycol 38:193-9. 2000
    ....
  25. ncbi Immune response in dogs experimentally infected with Paracoccidioides brasiliensis
    R C Eisele
    Departamento de Ciências Patológicas, Universidade Estadual de Londrina, Londrina, Brazil
    Med Mycol 42:549-53. 2004
    ..period, the immune responses of the dogs to the fungus were followed by ELISA (IgM and IgG), by the immunodiffusion test and by the skin test with gp43...
  26. ncbi Serological differentiation of Brucella-vaccinated and -infected domesticated animals by the agar gel immunodiffusion test using Brucella polysaccharide in mongolia
    Janchivdorj Erdenebaatar
    Laboratory of Bacteriology and Infectious Diseases, Immunological Research Center, Institute of Veterinary Medicine, Ulaanbaatar, Mongolia, Japan
    J Vet Med Sci 64:839-41. 2002
    ..serological reactions of Brucella-infected and -vaccinated domestic animals were compared by the agar gel immunodiffusion (AGID) test with a polysaccharide (poly-B) of the B. Abortus strain S-19...
  27. ncbi Evaluation of agar gel immunodiffusion and indirect fluorescent antibody assays as supplemental tests for dourine in equids
    J M Hagebock
    USDA, Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Ames, IA 50010
    Am J Vet Res 54:1201-8. 1993
    The agar gel immunodiffusion (AGID) and indirect fluorescent antibody (IFA) assays were evaluated as supplemental tests to the complement-fixation (CF) test, the official US importation certification test for dourine in equids...
  28. ncbi Evaluation of 5 serologic assays to detect Rhodococcus equi pneumonia in foals
    Ronald J Martens
    J Am Vet Med Assoc 221:825-33. 2002
    ....
  29. ncbi Comparison of serological tests based on outer membrane or internal antigens for detecting antibodies to Brucella ovis in infected flocks
    Silvia M Estein
    Departamento de Sanidad Animal y Medicina Preventiva, Facultad de Ciencias Veterinarias, UNICEN, Tandil, Argentina
    J Vet Diagn Invest 14:407-11. 2002
    ..Sera from 200 sheep from naturally infected flocks were assayed by agar gel immunodiffusion test (AGID) and by complement fixation test (CFT), both using HS, and by 4 ELISA using HS, R-LPS, CP, and p18,..
  30. ncbi Serodiagnosis of equine infectious anemia by agar gel immunodiffusion and ELISA using a recombinant p26 viral protein expressed in Escherichia coli as antigen
    Adriana S Toledo Piza
    Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of Sao Paulo, CEP 05508 270, Sao Paulo, SP, Brazil
    Prev Vet Med 78:239-45. 2007
    ..equine infectious-anemia virus (EIAV) expressed in Escherichia coli as antigen to standardize an agar-gel immunodiffusion (AGIDp26r) test and an indirect ELISA (ELISAp26r) for the detection of antibodies against EIAV in 720 equine ..
  31. ncbi Rapid immunochromatographic test for hantavirus andes contrasted with capture-IgM ELISA for detection of Andes-specific IgM antibodies
    Maritza Navarrete
    Department of Clinical Microbiology, Hospital Regional Valdivia, Valdivia, Chile
    J Med Virol 79:41-4. 2007
    ..Its usefulness for diagnosis is important in hospitals far from reference centers and areas with a high incidence of HPS cases...
  32. ncbi [Use of diagnostic DNA probes for detection of the provirus of bovine leukemia virus in infected cows]
    N A Mal'tseva
    Vopr Virusol 47:38-41. 2002
    ..The sera samples taken from the cattle under study were also tested using immunodiffusion assay (ID)...
  33. ncbi Characterization of the sigmaB-encoding genes of muscovy duck reovirus: sigmaC-sigmaB-ELISA for antibodies against duck reovirus in ducks
    Yun Zhang
    Avian Infectious Disease Division, National Key Laboratory of Veterinary Biotechnology, Harbin, Veterinary Research Institute of Chinese Academy of Agricultural Sciences, No 427 Maduan Street, Harbin 150001, PR China
    Vet Microbiol 121:231-41. 2007
    ..Taken together, the results demonstrated that sigmaB-sigmaC-ELISA was a sensitive and accurate method for detecting antibodies to DRV...
  34. ncbi An investigation of equine infectious anaemia infection in the central Anatolia region of Turkey
    O Yapklç
    Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, Campus Konya, Turkey
    J S Afr Vet Assoc 78:12-4. 2007
    ..A total of 406 serum samples were examined by agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA) for antibody to equine infectious anaemia virus (EIAV) ..
  35. ncbi Comparative study of PCR as a direct assay and ELISA and AGID as indirect assays for the detection of bovine leukaemia virus
    D Martin
    , Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
    J Vet Med B Infect Dis Vet Public Health 48:97-106. 2001
    ..with different prevalences of enzootic bovine leukaemia were chosen to study the concordance between agar gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) methods...
  36. ncbi Appearance of serum antibodies against the avian influenza nonstructural 1 protein in experimentally infected chickens and turkeys
    William G Dundon
    OIE, FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, Istituto Zooprofilattico Sperimentale delle Venezie, Italy
    Avian Dis 51:209-12. 2007
    ..This fact must be taken into consideration when using a DIVA system based on the identification of antibodies to the NS1 protein...
  37. ncbi The use of antigen-capture enzyme-linked immunosorbent assay (ELISA) for the diagnosis of rinderpest and peste des petits ruminants in ethiopia
    G Abraham
    Sebeta National Animal Health Research Centre, Addis Ababa, Ethiopia
    Trop Anim Health Prod 33:423-30. 2001
    ..paper is based on observations on the performance of the antigen detection ELISA compared to the agar gel immunodiffusion (AGID) test, which also differentiates rinderpest from peste des petits ruminants (PPR)...
  38. ncbi Antigen-induced production of interferon-gamma in samples of peripheral lymph nodes from sheep experimentally inoculated with Mycobacterium avium subsp. paratuberculosis
    J M Gwozdz
    Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand
    Vet Microbiol 84:243-52. 2002
    ..paratuberculosis rather than active infection...
  39. ncbi Serologic study on parvovirus b19 infection in childhood acute lymphoblastic leukemia during chemotherapy: clinical and hematologic implications
    Erik D Heegaard
    Department of Clinical Microbiology, University State Hospital, Rigshospitalet, Copenhagen, Denmark
    J Pediatr Hematol Oncol 24:368-73. 2002
    ....
  40. ncbi Anaphylactic transfusion reactions in haptoglobin-deficient patients with IgE and IgG haptoglobin antibodies
    Eiko Shimada
    Transfusion Information Department, The Japanese Red Cross Central Blood Center, Tokyo, Japan
    Transfusion 42:766-73. 2002
    ..Haptoglobin deficiency therefore might be a risk factor for NHTRs...
  41. ncbi Detection of antibodies in serum and egg yolk following infection of chickens with an H6N2 avian influenza virus
    Darrell W Trampel
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011 1240, USA
    J Vet Diagn Invest 18:437-42. 2006
    ..38). Anti-influenza antibodies were monitored by use of an agar gel immunodiffusion test and an ELISA for a period of 20 weeks after inoculation...
  42. ncbi Paratuberculosis in key deer (Odocoileus virginianus clavium)
    Charlotte F Quist
    Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA
    J Wildl Dis 38:729-37. 2002
    ..paratuberculosis was cultured from the feces of one live, asymptomatic deer. The results of this study provided sufficient evidence to consider the Key deer herd infected with M. avium subsp. paratuberculosis at very low prevalence...
  43. ncbi Serum IgG1 and IgG2 antibody responses to Porphyromonas gingivalis in patients with periodontitis
    V Booth
    Kings College London Dental Institute at Guy s, King s College and St Thomas Hospitals, Department of Periodontology, London, UK
    Oral Microbiol Immunol 21:93-9. 2006
    ..This work aimed to investigate serum IgG1 and IgG2 antibody responses of periodontitis patients to protein and carbohydrate-rich antigens of P. gingivalis...
  44. ncbi Specificity of absorbed ELISA and agar gel immuno-diffusion tests for paratuberculosis in goats with observations about use of these tests in infected goats
    R J Whittington
    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570
    Aust Vet J 81:71-5. 2003
    ..To determine the specificity of serological tests that are currently used in veterinary diagnostic laboratories in Australia for detection of Mycobacterium avium subsp paratuberculosis infection in goats...
  45. pmc Mass value assignment of total and subclass immunoglobulin G in a human standard anthrax reference serum
    V A Semenova
    Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Clin Diagn Lab Immunol 11:919-23. 2004
    ..Mass values (in milligrams per milliliter) for total IgG and IgG subclasses 1 to 4 were determined by radial immunodiffusion. Anti-PA-specific IgG assignment (in micrograms per milliliter) was done by consensus of two complementary ..
  46. ncbi Culture and serologic survey for Mycobacterium avium subsp. paratuberculosis infection among southeastern white-tailed deer (Odocoileus virginianus)
    William R Davidson
    Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens 30602, USA
    J Wildl Dis 40:301-6. 2004
    ..These six deer were seronegative when tested by agar gel immunodiffusion (AGID)...
  47. ncbi Evaluation of a LAM ELISA for diagnosis of paratuberculosis in sheep and goats
    S K Munjal
    Division of Pathology, Indian Veterinary Research Institute, Izatnagar 243122, UP, India
    Vet Microbiol 103:107-14. 2004
    ..and serum ELISA containing lipoarabinomanan (LAM) antigen was evaluated in sheep and goats versus agar gel immunodiffusion (AGID) test and polymerase chain reaction (PCR) using milk and lymph nodes...
  48. ncbi A new end-point for ELISA titrations
    Jose Vidal
    Departamento de Personalidad, Facultad de Psicologia, Universidad de Barcelona, Barcelona, Spain
    J Immunoassay Immunochem 25:371-83. 2004
    ..It is proposed that the reciprocal of the sample dilution at which the color intensifies can be used as a measure of the sample antibody content. This report verifies the validity and precision of that procedure...
  49. ncbi Inter-test comparison between filter paper absorbed blood eluate and serum for malaria serology by enzyme immunoassay: an operational feasibility
    Sukla Biswas
    Malaria Research Centre ICMR, Delhi, India
    J Immunoassay Immunochem 25:399-410. 2004
    ..Total IgG and IgM contents were also estimated in individual sera and filter paper eluate by single radial immunodiffusion (SRID). Assay results of both serum and filter paper eluates were compared...
  50. pmc Comparison of commercial enzyme-linked immunosorbent assays and agar gel immunodiffusion tests for the serodiagnosis of equine infectious anemia
    Julie Paré
    St Hyacinthe Laboratory, Canadian Food Inspection Agency, Saint Hyacinthe, Quebec J2S 8E3
    Can J Vet Res 68:254-8. 2004
    ..limit, and precision) of commercially available enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) kits in comparison with a reference AGID kit for the detection of equine infectious anemia (EIA) ..
  51. ncbi Competitive enzyme-linked immunosorbent assay for the detection of the antibodies specific to akabane virus
    Tomoyuki Tsuda
    Clinical Virology Section, Kyushu Research Station, National Institute of Animal Health, Kagoshima 891 0105, Japan
    J Vet Diagn Invest 16:571-6. 2004
    ..These results suggest that the C-ELISA is acceptable as a rapid and specific method for detecting antibodies to AKAV and is a potential alternative to the SN test...
  52. ncbi Evaluation of a new antibody-based enzyme-linked immunosorbent assay for the detection of bovine leukemia virus infection in dairy cattle
    Gustavo E Monti
    Universidad Austral de Chile, Instituto de Medicina Preventiva, Facultad de Ciencias Veterinarias, Valdiva, Chile
    J Vet Diagn Invest 17:451-7. 2005
    ..The M108 and S108 were compared with agar gel immunodiffusion (AGID), polymerase chain reaction and a commercial ELISA...
  53. ncbi Comparative efficacy of standard AGID, CCIE and competitive ELISA for detecting bluetongue virus antibodies in indigenous breeds of sheep and goats in Rajasthan, India
    Smriti Shringi
    Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Rajasthan Agricultural University, Bikaner 334 001, Rajasthan, India
    J Vet Sci 6:77-9. 2005
    ..virus (BTV) in 408 local breeds of sheep in Rajasthan state in India was investigated using standard agar gel immunodiffusion (AGID) test. Maximum seropositivities of 11.3% (13/115), 10.7% (13/121), 7.1% (11/155) and 5...
  54. ncbi Control and eradication programme of enzootic bovine leucosis (EBL) from selected dairy herds in Turkey
    I Burgu
    Ankara University, Faculty of Veterinary Medicine, Department of Virology, Ankara, Turkey
    Dtsch Tierarztl Wochenschr 112:271-4. 2005
    ..Thereafter, a total of 74,347 sera were tested for the presence of BLV specific antibodies. The serological results and detail of EBL control/eradication programme were shown in this paper...
  55. ncbi [Application of lectins for determination of antibodies to glycoproteins of leukemia virus in bovine blood serum]
    L V Pirohova
    Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv
    Ukr Biokhim Zh 74:97-101. 2002
    ..Moreover, the treatment of surface with FGA-P and PLA lectins increases the sensitivity of immune sensor to the specific antibodies in the comparison with the non-treated one...
  56. ncbi Anti-alphaGal-dependent complement-mediated cytotoxicity in metastatic melanoma
    James M G Larkin
    Institute of Cancer Research, Chester Beatty Laboratories bRheumatology Section, Imperial College London, UK
    Melanoma Res 16:157-63. 2006
    ..Serum complement levels were assayed by radial immunodiffusion. Anti-alphaGal immunoglobulin G and immunoglobulin M titres were measured by enzyme-linked immunosorbent ..
  57. pmc Performance of five serological assays for diagnosis of Rhodococcus equi pneumonia in foals
    Steeve Giguere
    Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610, USA
    Clin Diagn Lab Immunol 10:241-5. 2003
    ..immunosorbent assays (ELISAs) (ELISA-6939, ELISA-33701, ELISA-VapA, and ELISA-California) and an agar gel immunodiffusion test for diagnosis of Rhodococcus equi pneumonia in foals was evaluated...
  58. ncbi Comparison of the standard AGID test and competitive ELISA for detecting bluetongue virus antibodies in camels in Gujarat, India
    B S Chandel
    Department of Microbiology, College of Veterinary Science and Animal Husbandry, Gujarat Agricultural University, Sardar Krushinagar, India
    Trop Anim Health Prod 35:99-104. 2003
    The performance of the standard agar gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (cELISA) for the detection of serum antibody against bluetongue virus (BTV) in clinically healthy and diseased ..
  59. ncbi Experimental transmission of Bovine leukemia virus in cattle via rectal palpation
    Junko Kohara
    Hokkaido Animal Research Center, Shintoku, Hokkaido 081 0038, Japan
    Jpn J Vet Res 54:25-30. 2006
    ..Three of four steers developed antibodies against BLV as determined by agar-gel immunodiffusion (AGID) test between 7 to 10 weeks after the first rectal palpation using common sleeves from BLV-infected cow...
  60. ncbi The improvement of monitoring methods of cattle infection with bovine leukemia virus (BLV)
    J Rułka
    Department of Cell Pathology, National Veterinary Research Institute, al Partyzantow 57, 24 100 Puławy, Poland
    Pol J Vet Sci 6:40-2. 2003
    ..Both the DNA of env-BLV gene in NCR, V-NCR, FLK and in recombinated AG clone cells, as well as in the whole blood and in blood isolated leukocytes of dairy cattle, were detected with the nested-PCR...
  61. ncbi Testing for Mycobacterium avium subsp. paratuberculosis infection in asymptomatic free-ranging tule elk from an infected herd
    Elizabeth J Manning
    Johne s Testing Center, School of Veterinary Medicine, University of Wisconsin, 2015 Linden Drive, Madison, Wisconsin 53706, USA
    J Wildl Dis 39:323-8. 2003
    ..ante-mortem testing using three Johne's disease assays: enzyme linked immunosorbent assay (ELISA); agar gel immunodiffusion assay (AGID), and fecal culture. Isolation of Mptb was made from fecal samples in six of 45 elk (13%)...
  62. ncbi [Express diagnostics of bovine leucosis by immune sensor based on surface plasmon resonance]
    L V Pyrohova
    Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv
    Ukr Biokhim Zh 74:88-92. 2002
    ..The industrially manufactured BLV antigen for screening test in the agar gel immunodiffusion (AGID) required the additional purification in order to be used in immune sensor analysis...
  63. ncbi Evidence of bovine immunodeficiency virus in cattle in Turkey
    Sothy Meas
    Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    Jpn J Vet Res 51:3-8. 2003
    ..6% positive for anti-BLV gp51 antibodies by an immunodiffusion test...
  64. pmc Detection of serum antibodies to ovine progressive pneumonia virus in sheep by using a caprine arthritis-encephalitis virus competitive-inhibition enzyme-linked immunosorbent assay
    Lynn M Herrmann
    Animal Disease Research Unit, Agricultural Research Service, U S Department of Agriculture, Pullman, Washington 99164 6630, USA
    Clin Diagn Lab Immunol 10:862-5. 2003
    ..collected throughout the United States to determine the sensitivity and specificity of cELISA and agar gel immunodiffusion (AGID) based on immunoprecipitation (IP) of [35S]methionine-labeled OPPV antigens as a standard of comparison...
  65. ncbi Comparison of PLA with AGID and ELISA results in serology diagnosis of bovine leukosis
    E Buzała
    Department of Cell Pathology, National Veterinary Research Institute, al Partyzantow 57, 24 100 Puławy, Poland
    Pol J Vet Sci 6:9-11. 2003
    ..9% and 92.9% in ELISA and PLA respectively. The mean OD value for positive samples in groups II and III ranged between 0.943 and 0.215. Immunoperoxidase PLA is a specific and sensitive test for detection of BLV infections...
  66. ncbi Evaluation of an absorbed ELISA and an agar-gel immuno-diffusion test for ovine paratuberculosis in sheep in Australia
    E S G Sergeant
    AusVet Animal Health Services, 69 Turner Crescent, NSW 2800, Orange, Australia
    Prev Vet Med 61:235-48. 2003
    ....
  67. ncbi Association between bovine-leukosis virus seroprevalence and herd-level productivity on US dairy farms
    S L Ott
    USDA, Centers for Epidemiology and Animal Health, 2150 Centre Avenue, Building B, Mail Stop 2E5, Fort Collins, CO 80526 8117, USA
    Prev Vet Med 61:249-62. 2003
    ..1006 herds (in 20 states) with at least 30 dairy cows were interviewed during 1996. The agar-gel immunodiffusion test was used to detect serum antibodies to BLV...
  68. ncbi IgG1,IgG2 and IgE intrathecal synthesis in Angiostrongylus cantonensis meningoencephalitis
    Alberto Juan Dorta-Contreras
    Laboratorio Central de Líquido Cefalorraquídeo LABCEL, Facultad de Ciencias Medicas Dr Miguel Enriquez, Calle Ramón Pintó No 202 Luyanó Ciudad Habana CP10700, Cuba
    J Neurol Sci 238:65-70. 2005
    ..Angiostrongylus cantonensis meningoencephalitis is an emergent zoonotic disease in the Caribbean basin, characterized by the presence of eosinophils and third stage larva of the helmint...
  69. ncbi Evidence for bovine immunodeficiency virus infection in cattle in Zambia
    Sothy Meas
    Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    Jpn J Vet Res 52:3-8. 2004
    ..0% were positive for anti-BLV gp51 antibodies as detected by immunodiffusion test...
  70. ncbi The eradication of enzootic bovine leucosis in a large farm population
    W Dereń
    Department of Veterinary Hygiene, Wrocławska 170, 45 836 Opole, Poland
    Pol J Vet Sci 6:12-4. 2003
    ..The farm demonstrated BLV-free status after 10 years as a result of regular laboratory testing and successful elimination of all positive animals...
  71. ncbi Pre-clinical development of cell culture (Vero)-derived H5N1 pandemic vaccines
    M Keith Howard
    Baxter AG, Biomedical Research Center, Uferstrasse 15, A 2304 Orth Donau, Austria
    Biol Chem 389:569-77. 2008
    ..These data indicate that cell culture-grown, whole virus vaccines, based on the wild-type virus, allow the rapid high-yield production of a candidate pandemic vaccine...
  72. pmc Diagnostic detection methods for Mycobacterium avium subsp. paratuberculosis in white-tailed deer
    Murray R Woodbury
    Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4
    Can Vet J 49:683-8. 2008
    ..Deer identified as positive by agar gel immunodiffusion (AGID) testing or enzyme-linked immunosorbent assay (ELISA) but not by bacterial culture, Ziehl-Neelsen ..
  73. ncbi Enzyme-linked immunosorbent assay, single radial immunodiffusion, and indirect methods for the detection of failure of transfer of passive immunity in dairy calves
    S H Lee
    Department of Clinical Pharmacology and Therapeutics, University of Ulsan College of Medicine, Seoul, Korea
    J Vet Intern Med 22:212-8. 2008
    ..Enzyme-linked immunosorbent assay (ELISA) has advantages over single radial immunodiffusion (SRID) in terms of cost and time.
  74. ncbi Antibody index and specific antibody quotient in horses after intragastric administration of Sarcocystis neurona sporocysts
    Katherine A Heskett
    Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA
    Am J Vet Res 69:403-9. 2008
    ....
  75. ncbi Assessment of four serological techniques in the immunological diagnosis of farmers' lung disease
    Gabriel Reboux
    Department of Mycology, University Hospital, Besancon, France
    J Med Microbiol 56:1317-21. 2007
    ..WB analysis failed to identify specific bands for FLD. This study demonstrates the efficacy of determining precipitin levels with an appropriate technique, using a panel of antigens consistent with the specific exposure of a given area...
  76. ncbi Frequency of detectable serum IgG concentrations in precolostral calves
    Munashe Chigerwe
    Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA
    Am J Vet Res 69:791-5. 2008
    ....
  77. ncbi Plant-expressed HA as a seasonal influenza vaccine candidate
    Yoko Shoji
    Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Suite 200, Newark, DE 19711, USA
    Vaccine 26:2930-4. 2008
    ..The antigenicity of this plant-produced HA was confirmed by ELISA and single-radial immunodiffusion (SRID) assays...
  78. ncbi Clinical and serological heterogeneity in patients with anticentromere antibodies
    Shoji Miyawaki
    Rheumatic Diseases Center, Kurashiki Medical Center, Okayama, Japan
    J Rheumatol 32:1488-94. 2005
    ..To evaluate the clinical and serological heterogeneity in patients with anticentromere antibodies (ACA)...
  79. ncbi Improvements to the hemagglutination inhibition test for serological assessment of recombinant fowlpox-H5-avian-influenza vaccination in chickens and its use along with an agar gel immunodiffusion test for differentiating infected from noninfected vaccina
    David E Swayne
    Southeast Poultry Research Laboratory, Agricultural Research Service, U S Department of Agriculture, 934 College Station Road, Athens, Georgia 30605, USA
    Avian Dis 51:697-704. 2007
    ..and evaluated for correlation of immunological response by hemagglutination inhibition (HI) or agar gel immunodiffusion (AGID) tests and determination of protection following challenge with a high pathogenicity AI (HPAI) virus...
  80. ncbi Antinuclear antibodies: clinical correlations and biologic significance
    R D Sontheimer
    Department of Dermatology, University of Texas Southwestern Medical Center, Dallas
    Adv Dermatol 7:3-52; discussion 53. 1992
    ..For example, with classical immunodiffusion, anti-Ro/SS-A antibodies can be detected in 25% of unselected patients with SLE, whereas with an ELISA based ..
  81. ncbi Capture ELISA and flow cytometry methods for toxicologic assessment following immunization and cyclophosphamide challenges in beagles
    R D Jones
    Toxicology Department, Bayer Corporation, Stilwell, KS 66085, USA
    Toxicol Lett 115:33-44. 2000
    ..intra-assay reproducibility for IgM, IgG and IgE, with values in good agreement as reported for radial immunodiffusion. The immunologic challenge demonstrated a biological trend of an increase in IgM that preceded an increase in ..
  82. ncbi Seroepizootiological survey for selected viral infections in captive Asiatic lions (Panthera leo persica) from western India
    Anand Ramanathan
    Wildlife Institute of India, P O Box 18, Chandrabani, Dehra Dun, Uttar Pradesh 248001, India
    J Zoo Wildl Med 38:400-8. 2007
    ..Agar gel immunodiffusion test and dot immunobinding assay were employed for CDV and FPV antibody detection...
  83. ncbi Antigenic complementarity among AIDS-associated infectious agents and molecular mimicry of lymphocyte proteins as inducers of lymphocytotoxic antibodies and circulating immune complexes
    Robert S Root-Bernstein
    Department of Physiology, Michigan State University, 2174 Biomedical and Physical Sciences Building, East Lansing, MI 48824, USA
    J Clin Virol 31:S16-25. 2004
    ..It has therefore been proposed that cofactor infections may interact with HIV by producing complementary antigens that induce LCTA and CIC, and that the resulting immunological dysfunction is part of AIDS pathogenesis...
  84. ncbi Antibodies against oxidized low density lipoproteins in pregnant women
    L Fialova
    First Institute of Medical Chemistry and Biochemistry, Charles University, Prague, Czech Republic
    Physiol Res 51:355-61. 2002
    ..ELISA kits for determination of oLAb IgG, ACA IgG, MS AFP and MS HCG, MS SP1 was analyzed by single radial immunodiffusion. We did not find any differences in the levels of oLAb IgG in the first and second trimester in the women of ..
  85. ncbi Bronchogenic carcinoma and secondary aspergillosis--common yet unexplored: evaluation of the role of bronchoalveolar lavage-polymerase chain reaction and some nonvalidated serologic methods to establish early diagnosis
    Mohammed Shahid
    Section of Mycology and Molecular Biology, Department of Medical Microbiology, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh, Uttar Pradesh, India
    Cancer 113:547-58. 2008
    ..However, the frequency of secondary aspergillosis in bronchogenic carcinoma still has not been defined clearly in the literature...
  86. pmc Production and characterization of a monoclonal antibody to the complement fixation antigen of Coccidioides immitis
    M J Dolan
    Department of Research Immunology, San Antonio State Chest Hospital, Texas 78223
    Infect Immun 59:2175-80. 1991
    Detection of complement-fixing antibody to coccidioidin by using the complement fixation test or an immunodiffusion assay for complement-fixing antibody (IDCF) is widely viewed as the most useful immunodiagnostic test for ..
  87. pmc Decreased tear lactoferrin concentration in patients with chronic hepatitis C
    T Abe
    Department of Ophthalmology, Akita University School of Medicine, Akita City, Japan
    Br J Ophthalmol 83:684-7. 1999
    ..In this study tear lactoferrin levels were measured to investigate the dry eye condition of patients with chronic hepatitis C...
  88. ncbi LSA: a new liver-specific antigen in the rat. I. Purification and characterization
    A A Mihas
    Division of Digestive Diseases, University of Mississippi School of Medicine, Jackson
    Int Arch Allergy Immunol 98:35-49. 1992
    ..preparation gave a complete identity pattern with the original crude rat liver extract when tested by double immunodiffusion. This antigen has a molecular weight of 72...
  89. ncbi Association of increased levels of fibrinogen and the -455G/A fibrinogen gene polymorphism with chronic periodontitis
    Sinem E Sahingur
    Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, NY 14214, USA
    J Periodontol 74:329-37. 2003
    ..In this study, we investigated the distribution of the -455G/A polymorphism and the relationship of this specific genotype to fibrinogen levels in periodontitis patients...
  90. ncbi Epizootic hemorrhagic disease virus and bluetongue virus serotype distribution in white-tailed deer in Georgia
    D E Stallknecht
    College of Veterinary Medicine, University of Georgia, Athens 30602, USA
    J Wildl Dis 31:331-8. 1995
    ..Precipitating antibodies to the EHDV or BTV serogroups, as detected by agar gel immunodiffusion (AGID) tests, were present in 35%, 29%, and 39% of deer sampled in 1989, 1990, and 1991, respectively...
  91. ncbi Hard palate perforation: an unusual finding in paracoccidioidomycosis
    L G Castro
    Division of Dermatology, Hospital das Clinicas, Schools of Medicine and Dentistry, University of Sao Paulo, R Mato Grosso 128 cj 34, Sao Paulo SP, 01239 040 Brazil
    Int J Dermatol 40:281-3. 2001
    ..Paracoccidioidomycosis (PCM) serologic reactions tested positive for double immunodiffusion (DI), complement fixation (CF) 1 : 256 and counterimmunoelectrophoresis (CIE) 1 : 128...
  92. ncbi Anti-synthetase syndrome: a new autoantibody to phenylalanyl transfer RNA synthetase (anti-Zo) associated with polymyositis and interstitial pneumonia
    Z Betteridge
    University of Bath, Bath, UK
    Rheumatology (Oxford) 46:1005-8. 2007
    ..The aim of this study was to characterize the autoantibody profile in a patient with clinical features of ASS whose routine diagnostic testing was negative for the previously identified anti-synthetase autoantibodies...
  93. ncbi [Low prevalence of anti-centromere antibodies in scleroderma in Morocco (about 272 cases)]
    B Admou
    Faculte de Medecine, Marrakech, Morocco
    Ann Biol Clin (Paris) 65:291-7. 2007
    ..assessment of autoantibodies prevalence during scleroderma within a Moroccan population by a retrospective survey...
  94. ncbi Studies on the efficacy of different adjuvants in live stock specific bacterial vaccines for turkeys against Bordetella infection and onset of antibody titers in respect to the age of the turkey poults
    G Glünder
    Clinic for Poultry, School of Veterinary Medicine Hannover, Bunteweg 17, 30559 Hannover, Germany
    Pol J Vet Sci 7:77-81. 2004
    ..detected quantitatively using an ELISA for IgG and a microagglutination test for IgM and qualitatively using immunodiffusion. The damage at the application site was rated by measurement of the swelling of the tissue...
  95. ncbi Immunological comparison of subunits isolated from various hydrogenases of aerobic hydrogen bacteria
    B Lorenz
    Institut fur Mikrobiologie, Universitat Gottingen, F R G
    Biochim Biophys Acta 995:1-9. 1989
    ..By double immunodiffusion assays and tandem-crossed immunoelectrophoresis the large and the small subunit were demonstrated not to be ..
  96. ncbi Relation of serum retinol to acute phase proteins and malarial morbidity in Papua New Guinea children
    F J Rosales
    Nutrition and Veterinary Science Departments, The Pennsylvania State University, University Park, PA 16802, USA
    Am J Clin Nutr 71:1582-8. 2000
    ..Acute phase proteins (APPs) are associated with malaria-induced hyporetinemia (serum retinol <0.70 micromol/L); however, the degree of the association is not well documented...
  97. ncbi [Preparation and study of an exoantigen of Histoplasma capsulatum for serological reactions.]
    R Negroni
    Unidad de Micología del Hospital Francisco Javier Muñiz, Buenos Aires, Argentina
    Rev Iberoam Micol 15:282-5. 1998
    ..Proteins and polysaccharides concentrations were determined. Immunodiffusion (ID) tests were carried out with an antigenic dilution containing 1.4 mg/ml of proteins...
  98. ncbi Nonvitamin micronutrients
    Harvey Indyk
    Fonterra, No 1 Rd, PO Box 7, Waitoa, New Zealand
    J AOAC Int 89:288-9. 2006
    ..Although commercial radial immunodiffusion (RID) kits are available, they are generally variable in response...
  99. pmc Mutacin production by Streptococcus mutans may promote transmission of bacteria from mother to child
    L Gronroos
    Department of Pedodontics and Orthodontics, University of Helsinki, Finland
    Infect Immun 66:2595-600. 1998
    ..A total of 145 oral mutans streptococcal isolates were serotyped by immunodiffusion, ribotyped, and mutacin typed by the stab culture technique...
  100. ncbi Complement and myoblast transfer therapy: donor myoblast survival is enhanced following depletion of host complement C3 using cobra venom factor, but not in the absence of C5
    S I Hodgetts
    Department of Anatomy and Human Biology, University of Western Australia, Crawley, Western Australia, Australia
    Immunol Cell Biol 79:231-9. 2001
    ..Peripheral depletion of C3 was confirmed using double immunodiffusion, and local depletion of C3 in host TA muscles was confirmed by immunostaining of muscle samples...
  101. ncbi Serum IgA and IgG functional antibodies and their subclasses to Streptococcus pneumoniae capsular antigen found in two aged-matched cohorts of children with and without otitis media with effusion
    A B Drake-Lee
    The Department of Otorhinolaryngology, Birmingham Children s Hospital, Steelhouse Lane, Birmingham B4 6NH, UK
    Clin Otolaryngol Allied Sci 28:335-40. 2003
    ..The total IgG, IgA and all subclass levels were measured using radial immunodiffusion. Levels of functional IgA and IgG were measured using ELISAs (25 patients in each group)...

Research Grants24

  1. GENETIC ANALYSIS OF LUPUS AUTOIMMUNITY
    Judith James; Fiscal Year: 2000
    ..The antibodies that develop to Sm and nRNP are apparently of high titer in that they precipitate in double immunodiffusion assays and bind RNP antigens in solution phase and by immunoblots...
  2. TITER ON CHIP: ALTERNATIVE TO SRID FOR INFLUENZA VACCINE POTENCY DETERMINATION
    Kathy L Rowlen; Fiscal Year: 2013
    ..approved "gold standard" potency assay for influenza hemagglutinin protein based vaccines is single radial immunodiffusion (SRID)...
  3. AUTONOMIC CONTROL OF LACRIMAL GLAND FUNCTION
    BARRY BROMBERG; Fiscal Year: 1980
    ..Certain proteins such as IgG, secretory IgA, and secretory component will be quantitatively assayed by radial immunodiffusion. The capacity of isolated lacrimal cells to respond to autonomic agonists will be tested in cells recovered ..
  4. ENZYME IMMUNOASSAYS FOR THE DIAGNOSIS OF BLASTOMYCOSIS
    GENE SCALARONE; Fiscal Year: 1993
    ..to specificity and sensitivity have been attributed to the currently used complement fixation (CF) and immunodiffusion (ID) tests...
  5. ANIMAL BRUCELLOSIS: DIAGNOSTIC CONTROL METHODS
    LELAND CARMICHAEL; Fiscal Year: 1980
    ..with undiagnosed febrile illness; approximately 39% agglutinated the slide agglutination test antigen, while immunodiffusion tests revealed 13% seropositive. Approximately 11% of Mexican (D.R...
  6. APOLIPOPROTEIN-R MUTATION & PLASMA LIPID LEVELS IN PICS
    JAN RAPACZ; Fiscal Year: 1990
    ..Preparative ultracentrifugation, delipidation, gel affinity and gel chromatography, immunochemical and immunodiffusion techniques, native, SDS and 2D PAGE, will be employed to separate, purify, quantitate and identify Lpr ..
  7. PURIFICATION OF A FIBRINOLYSIS ENHANCING PROTEIN
    Mark Triscott; Fiscal Year: 1992
    ..The protein did not activate plasminogen and had no measurable fibrinolytic activity. Immunodiffusion analysis of the intact protein revealed non identity with vitronectin or histidine rich glycoprotein, other ..
  8. AIR POLLUTANTS AND CHRONIC RESPIRATORY DISEASE
    Gail Thomas; Fiscal Year: 1980
    ..the serum used in metabolic inhibition tests and also to detect concentrations of immunoglobulins by radial immunodiffusion. Comparison between pollutant-exposed and control mice are made using Student's t-test...
  9. REMOVAL OF SENESCENT AND STORED HUMAN RED CELLS
    Marguerite Kay; Fiscal Year: 1980
    ..Ig eluted from senescent cells and RBC stored as whole blood is an IgG free of other Ig's as determined by immunodiffusion, immunoelectrophoresis and polyacrylamide gel electrophoresis...
  10. A KINETIC STUDY OF INFLAMMATION-INDUCED HYPORETINEMIA
    Francisco Rosales; Fiscal Year: 2005
    ..In addition, the application of these methods and the development of a model of chronic inflammation will foster research on other micronutrients like iron and zinc that are similarly affected by inflammation. ..
  11. ADJUNCT VITAMIN A THERAPY FOR TUBERCULOSIS AND HIV/AIDS
    Richard Semba; Fiscal Year: 2005
    ....
  12. Sjogren's model with anti-Ro/La and Salivary Pathology and Dysfunction
    R Hal Scofield; Fiscal Year: 2010
    ..Improved understanding of the disease at a fundamental level is possible through study of animal models. Such understanding may lead to improved recognition and treatment. ..
  13. Hepcidin and the pathogenesis of anemia in older adults.
    Richard D Semba; Fiscal Year: 2010
    ..This study should provide new insight into the epidemiology and biological mechanisms of anemia among older adults and help identify strategies aimed at the prevention and treatment of anemia. ..
  14. The X chromosome in systemic lupus erythematosus
    R Hal Scofield; Fiscal Year: 2010
    ..This proposal will determine whether the presence of two X chromosomes is a risk factor for women and men with Klinefelter's syndrome (47,XXY). ..
  15. Oxidative Stress and the Pathogenesis of Sarcopenia and Disability in Older Women
    Richard Semba; Fiscal Year: 2008
    ..AGEs are a potentially modifiable risk factor, as circulating AGEs can be lowered by dietary modification and by pharmacological intervention. ..
  16. Host control in Coccidioidomycosis
    John Galgiani; Fiscal Year: 2008
    ..We will also analyze the phenotype, function and cytokine profiles of lymphocyte subsets that respond to antigen-pulsed DC. ..
  17. Genetics of Severe Systemic Lupus Erythematosus (SLE) defined by thrombocytopenia
    R Scofield; Fiscal Year: 2007
    ..Finally, Specific Aim 4 will assemble a new cohort of families multiplex for SLE so that linkages and associations as well as particular polvmorphisms can be prospectively confirmed. ..
  18. Nikkomycin Z Treatment for Coccidioidomycosis
    John Galgiani; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  19. Iron Deficiency and Anemia in HIV and HCV-Infected Women
    Richard Semba; Fiscal Year: 2005
    ..S. and elsewhere. ..
  20. MICRONUTRIENTS AND MOTHER TO CHILD TRANSMISSION OF HIV
    Richard Semba; Fiscal Year: 2001
    ..The relationship between mastitis and breastmilk HIV load and the epidemiology and microbiology of mastitis will be examined in a new cohort of HIV-infected lactating women. ..
  21. VITAMIN A SUPPLEMENTATION FOR HIV INFECTED CHILDREN
    Richard Semba; Fiscal Year: 2002
    ....
  22. MATERNAL IMMUNIZATION TO PREVENT INFANT OTITIS MEDIA
    Patricia Ferrieri; Fiscal Year: 2005
    ..Results of this trial will have a profound impact on the broad field of maternal immunization to prevent early infant disease caused by a variety of infant bacterial and viral pathogens. ..
  23. HUMAN EHRLICHIOSIS SURVEILLANCE AND EPIDEMIOLOGY
    William Davidson; Fiscal Year: 2003
    ....