Genomes and Genes
Summary: Serologic tests in which a positive reaction manifested by visible precipitation occurs when a soluble ANTIGEN reacts with its ANTIBODIES.
Publications248 found, 100 shown here
- Laminin mediates tissue-specific gene expression in mammary epitheliaC H Streuli
School of Biological Sciences, University of Manchester, Zurich
J Cell Biol 129:591-603. 1995..Our results demonstrate that laminin can direct tissue-specific gene expression in epithelial cells through its globular domain...
- Acf1, the largest subunit of CHRAC, regulates ISWI-induced nucleosome remodellingA Eberharter
Adolf Butenandt Institut, Molekularbiologie, Schillerstrasse 44, D 80336 MUnchen, Germany
EMBO J 20:3781-8. 2001..Refined analyses now suggest that topoisomerase II should not be considered a stable subunit of CHRAC. Accordingly, CHRAC can be molecularly defined as a complex consisting of ISWI, Acf1, CHRAC-14 and CHRAC-16...
- The primary structure of NG2, a novel membrane-spanning proteoglycanA Nishiyama
La Jolla Cancer Research Foundation, California 92037
J Cell Biol 114:359-71. 1991..The sequence of NG2 does not show significant homology with any other known proteins, suggesting that NG2 is a novel species of integral membrane proteoglycan...
- A novel quality control compartment derived from the endoplasmic reticulumS Kamhi-Nesher
Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel, 69978
Mol Biol Cell 12:1711-23. 2001....
- The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complexA Madania
Mécanismes Moléculaires de la Division Cellulaire et du Développement, Unité Propre de Recherche 9005 du Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, F 67084 Strasbourg, France
Mol Biol Cell 10:3521-38. 1999....
- RNA helicase A mediates association of CBP with RNA polymerase IIT Nakajima
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Cell 90:1107-12. 1997....
- The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338A J King
Department of Medicine, Indiana University School of Medicine, The Walther Oncology Center, Indianapolis 46202, USA
Nature 396:180-3. 1998..As guanine-nucleotide-exchange activity on Rac can be stimulated by a Ras-dependent phosphatidylinositol-3-OH kinase, a mechanism could exist through which one Ras effector pathway can be influenced by another...
- BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structuresY Wang
Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
Genes Dev 14:927-39. 2000..Collectively, these results suggest that BRCA1 may function as a coordinator of multiple activities required for maintenance of genomic integrity during the process of DNA replication and point to a central role for BRCA1 in DNA repair...
- Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunitsR N Gunawardane
Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
J Cell Biol 151:1513-24. 2000..We found that Dgrips128 and 163 can each interact with gammaTuSC. However, this interaction is insufficient for gammaTuRC assembly...
- Requirement of BMP-2-induced phosphatidylinositol 3-kinase and Akt serine/threonine kinase in osteoblast differentiation and Smad-dependent BMP-2 gene transcriptionNandini Ghosh-Choudhury
Department of Pathology, University of Texas Health Sciences Center, San Antonio, Texas 78229, USA
J Biol Chem 277:33361-8. 2002....
- Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cellsEnrica Audero
Division of Molecular Angiogenesis, Institute for Cancer Research and Treatment IRCC, School of Medicine, University of Torino, 10060 Candiolo, Italy
J Biol Chem 279:13224-33. 2004....
- Cdk2 knockout mice are viableCyril Berthet
Regulation of Cell Growth Laboratory, National Cancer Institute, Building 560, 1050 Boyles St, Frederick, MD 21702 1201, USA
Curr Biol 13:1775-85. 2003..In addition, Cdk2 associates with cyclin A, which itself is essential for cell proliferation during early embryonic development...
- Dissection of the dislocation pathway for type I membrane proteins with a new small molecule inhibitor, eeyarestatinEdda Fiebiger
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Biol Cell 15:1635-46. 2004..The new inhibitors must therefore interfere with a step that precedes proteasomal degradation. The use of eeyarestatin I thus allows the definition of a new intermediate in dislocation...
- Analysis of the native quaternary structure of vanilloid receptor 1N Kedei
Laboratory of Cellular Carcinogenesis and Tumor Promotion, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 276:28613-9. 2001..Finally, differences in glycosylation between the systems were observed, indicating the need for caution in the use of the heterologous expression systems for analysis of VR1 properties...
- The role of Xgrip210 in gamma-tubulin ring complex assembly and centrosome recruitmentL Zhang
Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
J Cell Biol 151:1525-36. 2000..These results suggest that Xgrip210 is a component of the gammaTuRC cap structure that is required for the assembly of the gammaTuRC...
- Three v-SNAREs and two t-SNAREs, present in a pentameric cis-SNARE complex on isolated vacuoles, are essential for homotypic fusionC Ungermann
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
J Cell Biol 145:1435-42. 1999..Our data show that vacuole-vacuole fusion requires a cis-SNARE complex of five SNAREs, the t-SNAREs Vam3p and Vam7p and the v-SNAREs Nyv1p, Vti1p, and Ykt6p...
- Hsl1p, a Swe1p inhibitor, is degraded via the anaphase-promoting complexJ L Burton
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520 8114, USA
Mol Cell Biol 20:4614-25. 2000....
- GM1 ganglioside-bound amyloid beta-protein in Alzheimer's disease brainK Yanagisawa
Department of Dementia Research, National Institute for Longevity Sciences, Morioka, Obu, Japan
Neurobiol Aging 19:S65-7. 1998..The present results strongly suggest that Abeta binds to a GM1 ganglioside in such a way that the bound Abeta is only recognized by BAN052, of the monoclonal antibodies used in this study...
- CREB-binding protein (CBP)/p300 and RNA polymerase II colocalize in transcriptionally active domains in the nucleusA von Mikecz
Junior Research Group of Molecular Cell Biology, Medizinisches Institut für Umwelthygiene, Heinrich Heine Universät Düsseldorf, 40225 Dusseldorf, Germany
J Cell Biol 150:265-73. 2000..The identification of pol II in CBP/PML-containing nuclear bodies supports the idea that transcription takes place at PML bodies...
- Intermediates in the formation of mouse 20S proteasomes: implications for the assembly of precursor beta subunitsD Nandi
Howard Hughes Medical Institute and Department of Molecular Genetics, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, OH 45267 0524, USA
EMBO J 16:5363-75. 1997..Finally, interferon-gamma had no significant effect on the half-lives or total amounts of precursor or mature proteasomes...
- Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendritesMitsutoshi Setou
Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
Nature 417:83-7. 2002..These results indicate that directly binding proteins can determine the traffic direction of a motor protein...
- A novel SNARE complex implicated in vesicle fusion with the endoplasmic reticulumM J Lewis
MRC Laboratory of Molecular Biology, Cambridge, UK
EMBO J 16:3017-24. 1997....
- Genomic targets of the human c-Myc proteinPaula C Fernandez
DNAX Research Institute, Palo Alto, California 94304, USA
Genes Dev 17:1115-29. 2003..Myc further enhanced histone acetylation, with or without accompanying induction of mRNA expression. Our findings point to a high regulatory and biological diversity among Myc-target genes...
- The alpha5beta1 integrin selectively enhances epidermal growth factor signaling to the phosphatidylinositol-3-kinase/Akt pathway in intestinal epithelial cellsJung Weon Lee
Department of Pharmacology, School of Medicine, University of North Carolina, 1106 Mary Ellen Jones Building, Chapel Hill, NC 27599 7365, USA
Biochim Biophys Acta 1542:23-31. 2002..Thus alpha5beta1 can support efficient activation of both the Erk and the phosphatidylinositol-3-kinase/Akt branches of the EGFR signaling cascade, whereas alpha2beta1 can support only the Erk branch...
- Hyperproliferation and defects in epithelial polarity upon conditional ablation of alpha-catenin in skinV Vasioukhin
Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, The University of Chicago, 5841 S Maryland Avenue, Chicago, IL 60637, USA
Cell 104:605-17. 2001..Thus, remarkably, features of precancerous lesions often attributed to defects in cell cycle regulatory genes can be generated by compromising the function of alpha-catenin...
- c-Cbl binding and ubiquitin-dependent lysosomal degradation of membrane-associated Notch1Birgit M Jehn
Friedrich Alexander University, Erlangen Nuremberg, Department of Experimental Medicine I, 91054 Erlangen, Germany
J Biol Chem 277:8033-40. 2002..Our data suggest a regulatory mechanism down-regulating Notch1 protein levels already at the cellular surface, possibly with consequences for Notch-dependent signal transduction during terminal differentiation processes...
- Serine phosphorylation of RUNX2 with novel potential functions as negative regulatory mechanismsHee Jun Wee
Department of Viral Oncology, Kyoto University, Japan
EMBO Rep 3:967-74. 2002..One CCD mutation, S104R of RUNX2, appears to mimic the phosphorylation-dependent inhibition of heterodimerization, thereby rendering RUNX2 metabolically unstable...
- The cyclin D1-dependent kinase associates with the pre-replication complex and modulates RB.MCM7 bindingAndrew B Gladden
Leonard and Madlyn Abramson Family Cancer Research Institute, Department of Cancer Biology, Abramson Family Cancer Center of the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 278:9754-60. 2003..Our data suggest that D1/CDK4 complexes play a direct role in altering an inhibitory RB.MCM7 complex possibly allowing for setting of the origin in preparation for DNA replication...
- Endocytosis of receptor tyrosine kinases is driven by monoubiquitylation, not polyubiquitylationYaron Mosesson
Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100, Israel
J Biol Chem 278:21323-6. 2003..Hence, receptor tyrosine kinases are desensitized through conjugation of multiple monoubiquitins, which is distinct from polyubiquitin-dependent proteasomal degradation...
- The presenilin 2 loop domain interacts with the mu-calpain C-terminal regionK Shinozaki
Laboratory of Neurochemistry, National Institute for Physiological Sciences, Myodaiji, Okazaki, Aichi 444, Japan
Int J Mol Med 1:797-9. 1998..In COS-1 cells, the interaction of PS2 with mu-calpain was confirmed by immunoprecipitation. These results suggested that PS2 and mu-calpain interact with each other, and might regulate each other's functions...
- CD151 enhances cell motility and metastasis of cancer cells in the presence of focal adhesion kinaseMasashi Kohno
First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Ehime 791 0295, Japan
Int J Cancer 97:336-43. 2002..These findings indicate that the CD151 molecule enhances cell motility, invasion and metastasis of cancer cells and that FAK is needed for these events through CD151...
- The Notch ligand Delta1 is sequentially cleaved by an ADAM protease and gamma-secretaseEmmanuelle Six
Unité de Biologie Moléculaire de l Expression Génique, Unité de Recherche Associée 2582, Centre National de la Recherche Scientifique, Institut Pasteur, 75724 Paris Cedex 15, France
Proc Natl Acad Sci U S A 100:7638-43. 2003..Therefore Dll1 is a substrate for regulated intramembrane proteolysis, and its intracellular region possibly fulfills a specific function in the nucleus...
- Apical sorting of beta-secretase limits amyloid beta-peptide productionAnja Capell
Adolf Butenandt Institute, Department of Biochemistry, Laboratory for Alzheimer s and Parkinson s Disease Research, Schillerstrasse 44, Ludwig Maximilians University, Munich 80336, Germany
J Biol Chem 277:5637-43. 2002..These data demonstrate that Abeta production is limited in polarized cells by differential targeting of BACE and its substrate betaAPP. Moreover, our findings suggest that betaAPP may not be a major physiological substrate of BACE...
- Recognition and ubiquitination of Notch by Itch, a hect-type E3 ubiquitin ligaseL Qiu
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
J Biol Chem 275:35734-7. 2000..Thus, Itch may participate in the regulation of immune responses by modifying Notch-mediated signaling...
- The Notch ligands, Jagged and Delta, are sequentially processed by alpha-secretase and presenilin/gamma-secretase and release signaling fragmentsMatthew J LaVoie
Center for Neurologic Diseases, Harvard Medical School and Brigham and Women s Hospital, Boston, Massachusetts 02215, USA
J Biol Chem 278:34427-37. 2003..Thus, Notch and its cognate ligands are processed by the same molecular machinery and may antagonistically regulate each other's signaling...
- Binding of c-Myc to chromatin mediates mitogen-induced acetylation of histone H4 and gene activationS R Frank
Department of Oncology, DNAX Research Institute, Palo Alto, California 94304, USA
Genes Dev 15:2069-82. 2001..Myc therefore governs a step, most likely H4 acetylation, that is required but not sufficient for transcriptional activation. We propose that Myc acts as a permissive factor, allowing additional signals to activate target promoters...
- The mammalian protein (rbet1) homologous to yeast Bet1p is primarily associated with the pre-Golgi intermediate compartment and is involved in vesicular transport from the endoplasmic reticulum to the Golgi apparatusT Zhang
Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 119076, Singapore
J Cell Biol 139:1157-68. 1997..Antibodies against rbet1 inhibit ER-Golgi transport only when they are added before the EGTA-sensitive stage. These results suggest that rbet1 may be involved in the docking process of ER-derived vesicles with the cis-Golgi membrane...
- Interaction of c-Jun amino-terminal kinase interacting protein-1 with p190 rhoGEF and its localization in differentiated neuronsD Meyer
Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
J Biol Chem 274:35113-8. 1999..The interaction of JIP-1 with rhoGEF and its subcellular localization suggests that JIP-1 may function to specifically localize a signaling complex in neuronal cells...
- Human T lymphocyte activation induces tyrosine phosphorylation of alpha-tubulin and its association with the SH2 domain of the p59fyn protein tyrosine kinaseA Marie-Cardine
Department of Applied Immunology, German Cancer Research Center, Heidelberg, Germany
Eur J Immunol 25:3290-7. 1995..Interestingly, constitutively tyrosine-phosphorylated alpha-tubulin that is able to interact with the fyn-SH2 domain is expressed in peripheral blood T lymphoblasts isolated from leukemic patients in the absence of external stimulation...
- E-cadherin binding prevents beta-catenin nuclear localization and beta-catenin/LEF-1-mediated transactivationS Orsulic
Max Planck Institut fur Immunbiologie, Stubeweg 51, D 79108 Freiburg, Germany
J Cell Sci 112:1237-45. 1999..The potent ability of E-cadherin to recruit beta-catenin to the cell membrane and prevent its nuclear localization and transactivation was also demonstrated using SW480 colon carcinoma cells...
- A biochemical function for the Sm complexD Zhang
Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02254, USA
Mol Cell 7:319-29. 2001..We speculate that this role may be general, and may have even evolved to aid weak intermolecular nucleic acid interactions of only a few base pairs...
- Heterotypic and homotypic associations between ezrin and moesin, two putative membrane-cytoskeletal linking proteinsR Gary
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853
Proc Natl Acad Sci U S A 90:10846-50. 1993..These results suggest mechanisms by which ezrin and moesin might participate in dynamic aspects of cortical cytoskeletal structure...
- Isolation of human delta-catenin and its binding specificity with presenilin 1H Tanahashi
Division of Demyelinating Disease and Aging, National Institute of Neuroscience, Kodaira, Tokyo, Japan
Neuroreport 10:563-8. 1999..These results suggest that PS1 and PS2 partly differ in function. PS1 loop fragment containing the pathogenic mutation retained the binding ability. We also found another armadillo-protein, p0071, interacted with PS1...
- Association of the transmembrane 4 superfamily molecule CD53 with a tyrosine phosphatase activityA M Carmo
MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, GB
Eur J Immunol 25:2090-5. 1995..These results demonstrate that the TM4SF members associate with tyrosine phosphatases. It seems possible that such associated phosphatases may contribute to the signal transduction capacity of TM4SF molecules...
- Itk/Emt/Tsk activation in response to CD3 cross-linking in Jurkat T cells requires ZAP-70 and Lat and is independent of membrane recruitmentX Shan
Laboratory of Biological Chemistry, Gerontology Research Center, NIA, National Institutes of Health, Baltimore, Maryland, 21224 6825, USA
J Biol Chem 274:29323-30. 1999..These data suggest that ZAP-70 and Lat play important, probably sequential, roles in regulating the activation of Itk following TCR engagement...
- Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactionsF Lund-Johansen
DNAX Research Institute for Cellular and Molecular Biology, Palo Alto, California, USA
Cytometry 39:250-9. 2000..We present a novel assay for the assessment of protein phosphorylation based on latex particles and flow cytometry...
- Functional link between BLM defective in Bloom's syndrome and the ataxia-telangiectasia-mutated protein, ATMHeather Beamish
Queensland Cancer Fund Research Laboratories, The Queensland Institute of Medical Research, P O Royal Brisbane Hospital, Herston, Brisbane, QLD 4029, Australia
J Biol Chem 277:30515-23. 2002..These data suggest that ATM and BLM function together in recognizing abnormal DNA structures by direct interaction and that these phosphorylation sites in BLM are important for radiosensitivity status but not for SCE frequency...
- Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2S Yonemura
Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 01, Japan
J Cell Biol 140:885-95. 1998..From these findings, we conclude that ERM proteins bind to integral membrane proteins bearing a positively charged amino acid cluster in their juxta-membrane cytoplasmic domain...
- Potential role for the BLM helicase in recombinational repair via a conserved interaction with RAD51L Wu
Imperial Cancer Research Fund Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
J Biol Chem 276:19375-81. 2001..Furthermore, genetic analysis reveals that the SGS1 and RAD51 genes are epistatic indicating that they operate in a common pathway. Potential roles for BLM in the RAD51 recombinational repair pathway are discussed...
- Vav3 mediates receptor protein tyrosine kinase signaling, regulates GTPase activity, modulates cell morphology, and induces cell transformationL Zeng
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA
Mol Cell Biol 20:9212-24. 2000..We conclude that Vav3 mediates RPTK signaling and regulates GTPase activity, its native and mutant forms are able to modulate cell morphology, and it has the potential to induce cell transformation...
- Evidence for covalent modification of the nuclear dot-associated proteins PML and Sp100 by PIC1/SUMO-1T Sternsdorf
Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, D 20251 Hamburg, Federal Republic of Germany
J Cell Biol 139:1621-34. 1997..Taken together, these data strongly suggest covalent modification of specific nuclear isoforms of Sp100 and PML by PIC1/SUMO-1. This modification may play a regulatory role in ND structure, composition, and function...
- TRPV3 is a temperature-sensitive vanilloid receptor-like proteinG D Smith
Neurology CEDD, GlaxoSmithKline, Third Avenue, Harlow CM19 5AW, UK
Nature 418:186-90. 2002..Hence, not only is VRL3 a thermosensitive ion channel but it may represent an additional vanilloid receptor subunit involved in the formation of heteromeric vanilloid receptor channels...
- Etk/Bmx as a tumor necrosis factor receptor type 2-specific kinase: role in endothelial cell migration and angiogenesisShi Pan
Center for Cardiovascular Research, University of Rochester Medical Center, Rochester, New York 14642, USA
Mol Cell Biol 22:7512-23. 2002..While most TNF actions have been attributed to TNFR1, our studies demonstrate that Etk is a TNFR2-specific kinase involved in TNF-induced angiogenic events...
- The pseudokinase domain is required for suppression of basal activity of Jak2 and Jak3 tyrosine kinases and for cytokine-inducible activation of signal transductionPipsa Saharinen
Haartman Institute, Department of Virology, Biomedicum Helsinki, Programme for Developmental and Reproductive Biology, University of Helsinki, Finland
J Biol Chem 277:47954-63. 2002..Thus, we propose that the JH2 domain contributes to both the uninduced and ligand-induced Jak-receptor complex, where it acts as a cytokine-inducible switch to regulate signal transduction...
- Familial Alzheimer disease-linked presenilin 1 variants enhance production of both Abeta 1-40 and Abeta 1-42 peptides that are only partially sensitive to a potent aspartyl protease transition state inhibitor of "gamma-secretase"Takeshi Ikeuchi
Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, Chicago, Illinois 60637, USA
J Biol Chem 278:7010-8. 2003....
- The presence of a single tyrosine residue at the carboxyl domain of vascular endothelial growth factor receptor-2/FLK-1 regulates its autophosphorylation and activation of signaling moleculesRosana D Meyer
Department of Ophthalmology, School of Medicine, Boston University, 715 Albany Street, Boston, MA 02118, USA
J Biol Chem 277:27081-7. 2002..Collectively, these results suggest that phosphorylation of tyrosine 1212 of VEGFR-2 plays a crucial role in the activation of VEGFR-2 and subsequently VEGFR-2-mediated angiogenesis...
- Characterization of the c-MYC-regulated transcriptome by SAGE: identification and analysis of c-MYC target genesAntje Menssen
Max Planck Institute of Biochemistry, Molecular Oncology, Independent Junior Research Group, Am Klopferspitz 18a, D 82152 Martinsried Munich, Germany
Proc Natl Acad Sci U S A 99:6274-9. 2002..The c-MYC-regulated genes/tags identified here will help to define the set of bona fide c-MYC targets and may have potential therapeutic value for inhibition of cancer cell proliferation, tumor-vascularization, and restenosis...
- A CBP binding transcriptional repressor produced by the PS1/epsilon-cleavage of N-cadherin is inhibited by PS1 FAD mutationsPhilippe Marambaud
Department of Psychiatry and Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029, USA
Cell 114:635-45. 2003..These data raise the possibility that FAD mutation-induced transcriptional abnormalities maybe causally related to the dementia associated with FAD...
- IL-7-dependent human leukemia T-cell line as a valuable tool for drug discovery in T-ALLJoao T Barata
Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Blood 103:1891-900. 2004..Exploitation of this cell line should allow the identification of molecular targets and promote the rational design and validation of antileukemia signaling inhibitors...
- Biochemistry and pharmacology of epitope-tagged alpha(1)-adrenergic receptor subtypesAleksandra Vicentic
Department of Pharmacology, 5017 Rollins Research Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
J Pharmacol Exp Ther 302:58-65. 2002..Because only monomers are detected by photoaffinity labeling, dimers and trimers observed on Western blots may be pharmacologically inactive...
- Structure of the gamma-tubulin ring complex: a template for microtubule nucleationM Moritz
Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, 513 Parnassus Avenue, San Francisco, California 94143, USA
Nat Cell Biol 2:365-70. 2000..Antibodies against gamma-tubulin label microtubule ends, but not walls, in centrosomes. These data are consistent with a template-mediated mechanism for microtubule nucleation by the gammaTuRC...
- Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassemblyZ S Zhao
Glaxo IMCB Group, Institute of Molecular and Cell Biology, Singapore 117609, Singapore
Mol Cell Biol 20:6354-63. 2000..We propose that GIT1 and FAK cooperate to promote motility both by directly regulating focal complex dynamics and by the activation of Rac...
- Role of Erv29p in collecting soluble secretory proteins into ER-derived transport vesiclesW J Belden
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
Science 294:1528-31. 2001..In vivo, export of gpalphaf from the ER was saturable and depended on the expression level of Erv29p. These results indicate that membrane receptors can link soluble cargo proteins to the COPII coat...
- BRCA1 regulates GADD45 through its interactions with the OCT-1 and CAAT motifsWenhong Fan
Department of Radiation Oncology, Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
J Biol Chem 277:8061-7. 2002..These findings indicate that BRCA1 can up-regulate its targeted genes through protein-protein interactions and provide a novel mechanism by which BRCA1 participates in transcriptional regulation...
- p95-APP1 links membrane transport to Rac-mediated reorganization of actinA Di Cesare
Cell Adhesion Unit DIBIT S Raffaele Scientific Institute, Via Olgettina 58, 20132 Milano, Italy
Nat Cell Biol 2:521-30. 2000..Our results show that p95-APP1 functionally interacts with Rac and localizes to endosomal compartments, thus identifying p95-APP1 as a molecular link between actin organization, adhesion, and membrane transport during cell motility...
- Subunit interactions in the twin-arginine translocase complex of Escherichia coliA Bolhuis
Department of Biological Sciences, University of Warwick, Coventry, UK
FEBS Lett 472:88-92. 2000..Finally, we show that TatA is stable in the absence of TatB and may participate in a separate complex lacking TatB in wild-type cells...
- The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domainM P de Caestecker
Laboratory of Cell Regulation and Carcinogenesis, NCI, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
J Biol Chem 275:2115-22. 2000..These data identify a novel function of the middle linker region of Smad4, and define the role of the SAD as an important locus determining the transcriptional activation of the Smad complex...
- Analysis of receptor signaling pathways by mass spectrometry: identification of vav-2 as a substrate of the epidermal and platelet-derived growth factor receptorsA Pandey
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
Proc Natl Acad Sci U S A 97:179-84. 2000..The strategy suggested here can be used for routine identification of downstream components of cell surface receptors in mammalian cells...
- Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APCG K Chan
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
J Cell Biol 146:941-54. 1999..The combined data indicate that hBUBR1 can potentially provide two checkpoint functions by monitoring CENP-E-dependent activities at the kinetochore and regulating cyclosome/APC activity...
- Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteinsS Münchow
ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
J Cell Sci 112:1511-8. 1999..This strongly suggests a direct role of Myo4p myosin as a transporter of localised mRNAs, convincingly supporting the concept of motor-protein based mRNA localisation...
- Role of alphavbeta3 integrin in the activation of vascular endothelial growth factor receptor-2R Soldi
Institute for Cancer Research and Treatment IRCC, Biology and Biochemistry, School of Medicine, University of Torino, 10100 Torino, Italy
EMBO J 18:882-92. 1999....
- The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosisS A Jablonski
Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
Chromosoma 107:386-96. 1998..This suggests that both kinases are concentrated near the surface of the kinetochore where they can monitor kinetochore-microtubule interactions...
- Complex formation between junctin, triadin, calsequestrin, and the ryanodine receptor. Proteins of the cardiac junctional sarcoplasmic reticulum membraneL Zhang
Department of Medicine and the Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
J Biol Chem 272:23389-97. 1997..Taken together, these results suggest that junctin, calsequestrin, triadin, and the ryanodine receptor form a quaternary complex that may be required for normal operation of Ca2+ release...
- HIP1, a human homologue of S. cerevisiae Sla2p, interacts with membrane-associated huntingtin in the brainM A Kalchman
Department of Medical Genetics, University of British Columbia, Vancouver, Canada
Nat Genet 16:44-53. 1997..This provides the first molecular link between huntingtin and the neuronal cytoskeleton and suggests that, in HD, loss of normal huntingtin-HIP1 interaction may contribute to a defect in membrane-cytoskeletal integrity in the brain...
- A novel ubiquitin-specific protease is dynamically associated with the PML nuclear domain and binds to a herpesvirus regulatory proteinR D Everett
Medical Research Council Virology Unit, Glasgow, UK
EMBO J 16:566-77. 1997..These results identify a novel, transitory component of ND10 and implicate a previously uncharacterized ubiquitin-dependent pathway in the control of viral gene expression...
- A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusionD F Seals
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755 3844, USA
Proc Natl Acad Sci U S A 97:9402-7. 2000..HOPS initially associates with vacuole SNAREs in "cis" and, after release by priming, hops to Ypt7p, activating this Ypt/Rab switch to initiate docking...
- Stem cell factor induces phosphatidylinositol 3'-kinase-dependent Lyn/Tec/Dok-1 complex formation in hematopoietic cellsT B van Dijk
Institute of Hematology, Erasmus University Rotterdam, Rotterdam, The Netherlands
Blood 96:3406-13. 2000..These findings suggest that p62Dok-1 may function as an important scaffold molecule in cKit-mediated signaling...
- Phosphorylation and rapid relocalization of 53BP1 to nuclear foci upon DNA damageL Anderson
The Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
Mol Cell Biol 21:1719-29. 2001..These results suggest a role for 53BP1 in the DNA damage response and/or checkpoint control which may involve signaling of damage to p53...
- Sorting nexin 6, a novel SNX, interacts with the transforming growth factor-beta family of receptor serine-threonine kinasesW T Parks
Laboratory of Cell Regulation and Carcinogenesis, NCI, the Diabetes Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 276:19332-9. 2001..These findings are the first evidence for the association of the SNX family of molecules with receptor serine-threonine kinases...
- Characterization of nucleophosmin (B23) as a Myc target by scanning chromatin immunoprecipitationK I Zeller
Program in Human Genetics and Molecular Biology and Department of Medicine, The Johns Hopkins Oncology Center and The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
J Biol Chem 276:48285-91. 2001..Hence, a scanning chromatin immunoprecipitation (SChIP) strategy is useful in analyzing functional transcription factor-binding sites...
- The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteinsW J Friesen
Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6148, USA
Mol Cell Biol 21:8289-300. 2001....
- She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3pF Bohl
ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
EMBO J 19:5514-24. 2000..She2p and She3p are the first examples of adapters for tethering a localized mRNA to the motor protein and might serve as prototypes for RNA-motor protein adapters...
- The growth factor granulin interacts with cyclin T1 and modulates P-TEFb-dependent transcriptionMainul Hoque
Department of Biochemistry and Molecular Biology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07013 1709, USA
Mol Cell Biol 23:1688-702. 2003..In addition, granulin was a substrate for CDK9 but not for the other transcription-related kinases CDK7 and CDK8. Thus, granulin is a cellular protein that interacts with cyclin T1 to inhibit transcription...
- Direct interaction of p21-activated kinase 4 with PDZ-RhoGEF, a G protein-linked Rho guanine exchange factorAna Barac
Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892 4330, USA
J Biol Chem 279:6182-9. 2004....
- Mechanism of Bruton's tyrosine kinase-mediated recruitment and regulation of TFII-ICatarina Sacristán
Department of Pathology, Program in Immunology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
J Biol Chem 279:7147-58. 2004..Our results provide a beginning step toward understanding the functional importance of the TFII-I-Btk pathway in B cell signaling and gene expression...
- Expression of functional Schistosoma mansoni Smad4: role in Erk-mediated transforming growth factor beta (TGF-beta) down-regulationAhmed Osman
Department of Microbiology and Immunology and Witebsky Center for Microbial Pathogenesis and Immunology, School of Medicine, State University of New York, Buffalo, New York 14214, USA
J Biol Chem 279:6474-86. 2004..SmSmad4 was shown to complement a human Smad4 deficiency through the restoration of TGF-beta-responsiveness in MDA-MB-468 breast cancer cells...
- The N-terminal 24 amino acids of the p55 gamma regulatory subunit of phosphoinositide 3-kinase binds Rb and induces cell cycle arrestXianmin Xia
Greenebaum Cancer Center, University of Maryland, Baltimore, Maryland 21201, USA
Mol Cell Biol 23:1717-25. 2003..These data demonstrate for the first time an association of p55gamma with Rb and show that modification of this association can lead to cell cycle arrest...
- Characterization of insulin inhibition of transactivation by a C-terminal fragment of the forkhead transcription factor Foxo1 in rat hepatoma cellsValerie Perrot
Growth and Development Section, Diabetes Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 278:26111-9. 2003..These different regulatory mechanisms allow the precise control of transcription of Foxo1 target genes by insulin...
- Beta-catenin binds to the activation function 2 region of the androgen receptor and modulates the effects of the N-terminal domain and TIF2 on ligand-dependent transcriptionLiang Nian Song
Department of Oncology, Lombardi Cancer Center, Georgetown University School of Medicine, Washington, D C 20007 2197, USA
Mol Cell Biol 23:1674-87. 2003..Taken together, the data show that beta-catenin can bind to the androgen receptor LBD and modulate the effects of the androgen receptor NTD and TIF2 on transcription...
- MAPK signaling up-regulates the activity of hypoxia-inducible factors by its effects on p300Nianli Sang
Cardeza Foundation for Hematological Research, Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
J Biol Chem 278:14013-9. 2003..Taken together, our data suggest that MAPK signaling facilitates HIF activation through p300/CBP...
- The chaperone protein 14-3-3 interacts with 3BP2/SH3BP2 and regulates its adapter functionIsabelle Foucault
INSERM U343 and Laboratoire d Immunologie, hôpital de l Archet I, Nice 06202, France
J Biol Chem 278:7146-53. 2003..Indeed, a 3BP2 mutant protein incapable of binding 14-3-3 showed increased capacity to stimulate NFAT transcriptional activities, suggesting that 14-3-3 binding to 3BP2 negatively regulates 3BP2 adapter function in lymphocytes...
- The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor geneKaren E Sukhodolets
Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1802, USA
Mol Cell Biol 23:493-509. 2003..This finding was consistent with the extensive overlap in the nuclear localization patterns of endogenous menin, RPA2, and RPA1 observed by immunofluorescence...
- A novel ubiquitin fusion system bypasses the mitochondria and generates biologically active Smac/DIABLOAllison M Hunter
Solange Gauthier Karsh Molecular Genetics Laboratory, Children s Hospital of Eastern Ontario, Research Institute, 401 Smyth Road, Ottawa, Ontario K1H 8L1, Canada
J Biol Chem 278:7494-9. 2003..The ability to circumvent the requirement for mitochondrial processing and release is critical to developing Smac as a possible gene therapy payload in cancer chemosensitization...
- Post-translational modification of Rta of Epstein-Barr virus by SUMO-1Li Kwan Chang
Faculty of Biological Medicine and Environmental Biology and Graduate Institute of Biochemistry, Kaohsiung Medical University, 100, Shih Chuan 1st Road, Kaohsiung, Taiwan
J Biol Chem 279:38803-12. 2004..These results indicate that sumoylation of Rta may be important in EBV lytic activation...
- Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cellsKohji Noguchi
Department of Bioactive Molecules, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
J Biol Chem 279:32716-27. 2004..Nek2 dissociated this autoinhibitory interaction. Altogether, our studies demonstrate a unique mechanism of Nek11 activation by Nek2A in G(1)/S-arrested cells and suggest a novel possibility for nucleolar function of the NIMA family...
- A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1Aya Sasaki
Department of Geriatric Research, National Institute for Longevity Sciences, Obu, Aichi 474 8522, Japan
J Biol Chem 277:22541-6. 2002..These results suggest that Praja1 regulates the transcription function of the homeodomain protein Dlx5 by controlling the stability of Dlxin-1 via an ubiquitin-dependent degradation pathway...
- Development of an efficient "substrate-trapping" mutant of Src homology phosphotyrosine phosphatase 2 and identification of the epidermal growth factor receptor, Gab1, and three other proteins as target substratesYehenew M Agazie
Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, 11794 5222, USA
J Biol Chem 278:13952-8. 2003..Therefore, the DM protein would serve as an important tool in future SHP2 studies, including identification of p190, p150, and p90...
- PIAS3 suppresses NF-kappaB-mediated transcription by interacting with the p65/RelA subunitHyun Duk Jang
Division of Molecular Life Sciences and Center for Cell Signaling Research, Ewha Womans University, Seoul 120 750, Korea
J Biol Chem 279:24873-80. 2004..Taken together, these data suggest that PIAS3 may function in vivo as a modulator in suppressing the transcriptional activity of p65...
- The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activityKunihiko Takeyama
Department of Medical Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
J Biol Chem 278:21930-7. 2003..Consistent with this idea, BBAP and DTX1 associate via their unique N termini, resulting in enhanced self-ubiquitination...
- The hepatitis E virus open reading frame 3 protein activates ERK through binding and inhibition of the MAPK phosphataseAnindita Kar-Roy
Virology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India
J Biol Chem 279:28345-57. 2004..Expression of pORF3 protected ERK from the inhibitory effects of ectopically expressed Pyst1. This is the first example of a viral protein regulating ERK activation by inhibition of its cognate dual specificity phosphatase...
- A Cortactin-CD2-associated protein (CD2AP) complex provides a novel link between epidermal growth factor receptor endocytosis and the actin cytoskeletonDanielle K Lynch
Cancer Research Program, Garvan Institute of Medical Research, St Vincent s Hospital, Sydney, New South Wales 2010, Australia
J Biol Chem 278:21805-13. 2003..Therefore, by binding both CD2AP and the Arp2/3 complex, cortactin links receptor endocytosis to actin polymerization, which may facilitate the trafficking of internalized growth factor receptors...
- Redox Signaling in Osteoblast DifferentiationNandini Ghosh Choudhury; Fiscal Year: 2009..Understanding the signal transduction pathways of osteoblast differentiation may result in the development of therapeutic modalities for the human diseases where lack of bone formation is the pathology. ..
- 17a-OH progesterone caproate and progesterone actions in human myometrial cellsMELVYN SOLOFF; Fiscal Year: 2008..Unraveling these intricate pathways is essential to understanding biological mechanisms that control myometrial function and could lead to the development of improved and/or novel tocolytics. [unreadable] [unreadable] [unreadable]..
- Immortalization of Pregnant Human Myometrial CellsMELVYN SOLOFF; Fiscal Year: 2004....
- Antiretroviral-Induced Defects in Muscle Protein SynthesisCharles H Lang; Fiscal Year: 2010..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
- Cyclin D1 and mammary carcinomaJ Diehl; Fiscal Year: 2009..The work proposed herein will establish the relationship between cyclin D1 nuclear retention and the function of cyclin D1 as an oncogenic protein in mammary carcinoma. ..
- MMPs and Synaptic Injury with HIV/METHKatherine Conant; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Receptors for clostridial neurotoxinsEDWIN CHAPMAN; Fiscal Year: 2008..The studies proposed here will provide new insights into the molecular mechanism of action of the BoNTs, and may provide a novel means to prevent poisoning by these substances. ..
- ASM Conference on Candida and CandidiasisAaron Mitchell; Fiscal Year: 2008..The conference will help chart the course of future research and facilitate the urgent necessity to understand, treat, and prevent disease. [unreadable] [unreadable] [unreadable]..
- Role of Clic in Epithelial MorphogenesisMark Berryman; Fiscal Year: 2008..The fruit fly will be used as a model system to understand how Clic works with other genes and proteins known to be important for cell shape and movement during embryonic development. [unreadable] [unreadable] [unreadable]..
- Vascular Biology of Tissue FactorWolfram Ruf; Fiscal Year: 2008..These studies have, and will continue to produce original data and advanced knowledge of the TF driven thrombotic and inflammatory diseases and new routes to reduce the impact of these diseases. ..
- Genetics of Autontibody DiversificationUmesh Deshmukh; Fiscal Year: 2008..The proposal addresses a significant issue in autoimmunity and will serve well as a training vehicle to obtain the stated goals by the PI. ..
- The role of hMLH1-hMRE11 in DNA mismatch repairChengtao Her; Fiscal Year: 2007..abstract_text> ..
- MMPs in neural networkingKatherine Conant; Fiscal Year: 2006..The results of our studies should improve our understanding of those CNS inflammatory diseases that are characterized by elevated levels of MMPs. [unreadable] [unreadable] [unreadable]..
- VASCULAR BIOLOGY IN CANCERThomas Edgington; Fiscal Year: 2006..Weekly seminars and review of data are conducted by each of the faculty members with their group. M.D., Ph.D. and M.D./Ph.D. candidates are recruited and prepared for an independent investigative career. ..
- Oxidation and Pharmacologic Activation of IK/SK ChannelsDANIEL DEVOR; Fiscal Year: 2009..abstract_text> ..
- Biological Role of the FE65 Protein/APP InteractionsSuzanne Guenette; Fiscal Year: 2009..In addition, we will gain insight into the role of the FE65 proteins in cellular signaling events that are critical for brain function. ..
- HSP27 Association with Thin Filaments in Colonic MuscleKHALIL BITAR; Fiscal Year: 2009..abstract_text> ..
- MYOCARDIAL PROTEIN SYNTHESIS AFTER ALCOHOL INTOXICATIONCharles H Lang; Fiscal Year: 2010..Overall, the research design will establish the mechanism by which myocardial protein synthesis is reduced in response to alcohol abuse. ..
- Signaling by p21-activated protein kinasesJONATHAN D CHERNOFF; Fiscal Year: 2010....
- Myocardial Adenosine ReceptorsJames G Dobson; Fiscal Year: 2010..Such information should be instrumental for the development of new therapies and agents to alleviate cardiac dysfunction. ..
- NEUROTROPHIC FACTOR MODULATION OF CORNEAL ENDOTHELIUMShay Whey Koh; Fiscal Year: 2009..abstract_text> ..
- Critical Role of Nrf2 in Innate Immune Response and Survival During SepsisShyam Biswal; Fiscal Year: 2010..and survival during sepsis. These studies may lead to the development of novel strategies based on Nrf2 for intervening in sepsis and improving survival. ..
- HIV-1 gp120-mediated injury in brain endotheliumShalom Avraham; Fiscal Year: 2009..These studies should provide insights into preventive and/or therapeutic strategies to sustain BBB integrity in HIV-infected patients, and thereby contribute to limiting CNS pathology. ..
- VTC Events Mediated by the Rab2 ComplexEllen J Tisdale; Fiscal Year: 2010..The studies proposed in this application will develop a new and novel paradigm that couples Rab2-Apkc lA-GAPDH dysfunction with pathological consequences. ..
- Role of the Archipelago gene in Drosophila tracheal morphogenesisKenneth H Moberg; Fiscal Year: 2010..Thus, we hypothesize that ago plays an important role in the homeostatic mechanisms that restrain tracheal branching. Our goal in Specific Aim 3 is to define this role and elucidate its molecular basis. ..
- Mechanisms of CMV latency in accelerated vascular diseaseDaniel N Streblow; Fiscal Year: 2010..Does mutation of the RCMV genes expressed in the latently infected allografts alter virus-induced acceleration of disease? B. What is the function of RCMV genes expressed in latently infected allografts? ..
- Galanin and GalR2 Receptors in Antidepressant TreatmentsTamas Bartfai; Fiscal Year: 2009..The planned work will deepen our knowledge of the galanin/5-HT system in the DRN and its contribution to the effects of antidepressant treatments. ..
- Modifier Role of Nrf2 in Lung Injury and EmphysemaShyam Biswal; Fiscal Year: 2009..4) To test if overexpression of Nrf2 can inhibit emphysema. These studies promise to shed new light on novel pathways that modulate susceptibility to emphysema. ..
- Synaptotagmin in Excitation-CouplingEDWIN CHAPMAN; Fiscal Year: 2006..Finally, defining this mechanism should ultimately provide targets for treatment of diseases in which synaptic transmission is impaired. ..
- Transciption-independent Signaling by IL1 in NeuronsTamas Bartfai; Fiscal Year: 2006..molecular mechanisms of action will contribute to a better understanding of the neuronal effects of IL-1Beta in the hypothalamus, where it regulates the HPA axis, the temperature setpoint, and fever response [unreadable] [unreadable]..
- Activation of inflammatory cells by flagellinSTEVEN MIZEL; Fiscal Year: 2006..Furthermore, the proposed studies should also provide insights into the factors that regulate TLR signaling and allow for complex patterns of host cell response. ..
- Characterization of intestinal adenosine A2b receptorShanthi Sitaraman; Fiscal Year: 2004..Specific aim 3: We will study: i) the effect of IL-6 on degranulation of neutrophils. ..
- Role of the JIP-1-p190RhoGEF Complex In Neuron FunctionWILLIAM ARTHUR; Fiscal Year: 2004..Furthermore, the importance of the JIP-l-p190RhoGEF association in brain development and neuronal migration will be investigated by disrupting this complex in mutant mice . ..
- ADENOSINE A1 AND A2 RECEPTORS IN THE MYOCARDIUMJames Dobson; Fiscal Year: 2004..Additionally, the findings should significantly contribute to our basic knowledge of the molecular mechanisms of G protein coupled receptor cross-regulation in the heart. ..
- Cell-based screens for Alzheimer's disease therapeuticsPaul Mathews; Fiscal Year: 2004..Additionally, once a compound is identified as effective in a cell-based system it has a much greater probability of in vivo efficacy than a compound identified, for example, using a purified enzyme screen. ..
- PHOSPHORYLATION OF HSP 72 AND CARDIAC PROTECTIONANNE KNOWLTON; Fiscal Year: 2001..These studies will further the ultimate goal of understanding the regulation and function of this endogenous protective protein so that expression can be manipulated for the benefit of cardiac patients. ..
- MMPS IN HIV DEMENTIA: EFFECTS ON BBB STRUCTURE/FUNCTIONKatherine Conant; Fiscal Year: 2003..Also, the proposed studies will use both primary brain-derived HIV isolates, which differ from laboratory adapted strains, and normal human cells, which also differ significantly from those of rodent or tumor origin. ..
- HIV 1 PROTEINS AND DRUGS OF ABUSE AND METALLOPROTEASESKatherine Conant; Fiscal Year: 2003..We will also examine the mechanisms by which these substances may affect MMP production, and identify clinically tolerable compounds which may inhibit such production. ..
- Enzymatic matrix oxidation imaging and amyloidosis-BJanusz Frackowiak; Fiscal Year: 2002..1 ..
- ROLE OF THE NUCLEAR MATRIX PROTEIN NRB/B IN BRAINShalom Avraham; Fiscal Year: 2002..These studies should provide novel insights into the role of this nuclear matrix protein in neuronal differentiation. ..
- A Rapid Assay System for Photoreceptor Gene FunctionsMinoru Hasegawa; Fiscal Year: 2004..Successful completion of this proposed research will dramatically accelerate functional genomics research in vertebrate photoreceptors. ..
- Molecular Mechanisms of Receptor Function in SESEVERN CHURN; Fiscal Year: 2005..Understanding the cellular mechanisms that result in altered GABAAR function in SE is essential to the development of new treatment strategies to manage SE in the clinical setting. ..
- Mapping disease-specific human protein networksJoel Bader; Fiscal Year: 2005..Success in Phase I of this project will result in improved methods for mapping disease-relevant human biological networks. Success in Phase II will result in targets for therapeutic intervention. ..
- Role of Acid-Sensing Ion Channels in GlaucomaJulie Saugstad; Fiscal Year: 2006..abstract_text> ..
- UNDERSTANDING SPINOCEREBELLAR ATAXIA TYPE 1 PATHOGENESISPuneet Opal; Fiscal Year: 2005..neuronal damage are hallmarks of not just the polyglutamine disorders but also diseases such as Parkinson's and Alzheimer's, so the results of this project should have broad ramifications for a number of neurodegenerative conditions ..
- Intracellular Signaling Triggered by Tall-1/BCMAHong Bing Shu; Fiscal Year: 2005..The genes and their protein products identified in this study may be used as molecular targets for drug development against certain immune-deficient and autoimmune diseases. ..
- Protective Mechanism of an Anti-Inflammatory Herbal MixAli Banan; Fiscal Year: 2006..abstract_text> ..
- Myocardial Protein Synthesis After Thermal InjuryCharles Lang; Fiscal Year: 2005..Overall, the research will elucidate the mechanisms by which myocardial protein synthesis is reduced after thermal injury, leading to the better understanding and treatment of the resulting cardiomyopathy. - ..
- HoxA10 Function During Myeloid DifferentiationElizabeth Eklund; Fiscal Year: 2006..These investigations will identify the molecular mechanisms of target gene regulation by HoxA10, which may suggest a role for HoxA10 in differentiation block in myeloid leukemia. ..
- ICSBP Function During Myeloid DifferentiationElizabeth Eklund; Fiscal Year: 2006..These studies will determine if ICSBP tyrosine phosphorylation is necessary for myeloid differentiation and leukemia suppression, providing a unique model to dissect early events in the evolution of myeloid malignancy. ..
- TRANSCRIPTIONAL CONTROL OF INTERFERON-GAMMA IN T AND NK CELLSAMY SUSAN WEINMANN; Fiscal Year: 2010....
- BIOLOGICAL FUNCTION OF XRCC2 IN RECOMBINATIONAL REPAIRNan Liu; Fiscal Year: 2003..This study will lead to a better understanding of the role of XRCC2 in mammalian HRR and the importance of this pathway in maintaining chromosomal stability. ..
- Regulatory subunits of PP2A in neuronal functionStefan Strack; Fiscal Year: 2011..These studies are significant in that they advance our understanding of the critical regulatory enzyme PP2A with the ultimate goal of better therapies for several neurodegenerative disorders. ..
- Calpain Regulation of Amyloid-beta and Plaque FormationPaul Mathews; Fiscal Year: 2007....
- ABC-transporter binding proteins and traffickingDaniel F Ortiz; Fiscal Year: 2010..abstract_text> ..
- A functional array for signature of breast cancerXin Jiang; Fiscal Year: 2005....
- Extracellular Modulation of Metabotropic GluRsJulie Saugstad; Fiscal Year: 2005..abstract_text> ..
- PROTEIN SYNTHESIS INITIATION IN ANIMAL CELLSBansidhar Datta; Fiscal Year: 2002..This research is expected to provide valuable information regarding the mechanism of protein synthesis initiation in normal, cancerous, and virus- infected animal cells. ..
- CELLULAR REGULATION OF PROSTAGLANDIN SYNTHESISAndrey Sorokin; Fiscal Year: 2009....
- ENDOTHELIN SIGNALING AND REGULATION OF PROTEIN KINASESAndrey Sorokin; Fiscal Year: 2006..We hypothesize that MKP-3 inhibits ET-1 mediated ERK activation, whereas MKP-1 attenuates p38 MAPK and tight regulation of MAPK signaling by MKP-1 and MKP-3 controls expression of genes critical for GMC hypertrophy. ..
- Lung Cancer Chemoprevention using DeguelinHo Young Lee; Fiscal Year: 2008..abstract_text> ..
- HETEROGENEITY OF CAVEOLAE IN INTESTINAL EPITHELIAKamran Badizadegan; Fiscal Year: 2004..Furthermore, by using signal transduction by cholera toxin (CT) in intestinal epithelia as a classical model of DIG-mediated function, these studies extend our understanding of the pathogenesis of enterotoxicrenic secretory diarrhea. ..
- REGULATION OF P21-ACTIVATED KINASESJonathan Chernoff; Fiscal Year: 2004..Understanding the molecular basis for this regulation is not only of intrinsic scientific interest but is also likely to be relevant to important human diseases such as metastatic cancer. ..
- The Role of Cell Size and G1-phase Cdks in Cell Growth ControlBrandt L Schneider; Fiscal Year: 2010..This exemplifies the critical importance of understanding the role of G1-phase Cdks in proliferation because this type of information is essential before effective anti-cancer clinical interventions can be designed. ..
- A Growth-Regulating Protein Tyrosine PhosphataseJonathan Chernoff; Fiscal Year: 2007..Importantly, it also may point the way to the development of new therapeutic agents for diseases such as diabetes and cancer. ..
- Tumor metastasis: Biobehavioral mechanismsAnil K Sood; Fiscal Year: 2009....
- Retrotransposons as site-specific gene delivery vectorsJOHN GOODIER; Fiscal Year: 2003..In addition, I will assay in cell culture the efficiency of the L1 at integrating increasing lengths of exogenous DNA. ..