immunophenotyping

Summary

Summary: Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.

Top Publications

  1. ncbi Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival
    Tyler J Curiel
    Tulane University Health Science Center, New Orleans, Louisiana 70112, USA
    Nat Med 10:942-9. 2004
  2. ncbi Isolation of multipotent mesenchymal stem cells from umbilical cord blood
    Oscar K Lee
    Department of Orthopaedics and Traumatology, Veterans General Hospital Taipei, Taipei 11221, Taiwan
    Blood 103:1669-75. 2004
  3. pmc Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo
    S Gronthos
    Craniofacial and Skeletal Diseases Branch, National Institute on Dental Research, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:13625-30. 2000
  4. ncbi Mesenchymal progenitor cells in human umbilical cord blood
    A Erices
    Unidad de Biologia Celular, INTA, Universidad de Chile, Santiago, Chile
    Br J Haematol 109:235-42. 2000
  5. ncbi Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood
    Wolfgang Wagner
    Department of Medicine V, University of Heidelberg, Heidelberg, Germany
    Exp Hematol 33:1402-16. 2005
  6. ncbi Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells
    Mauro Krampera
    Department of Clinical and Experimental Medicine, University of Verona, Italy
    Stem Cells 24:386-98. 2006
  7. doi Neutrophils and granulocytic myeloid-derived suppressor cells: immunophenotyping, cell biology and clinical relevance in human oncology
    Claudia A Dumitru
    Department of Otorhinolaryngology, University of Duisburg Essen, Hufelandstrase 55, Essen, Germany
    Cancer Immunol Immunother 61:1155-67. 2012
  8. ncbi Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow
    C Campagnoli
    Department of Maternal and Fetal Medicine, Institute of Reproductive and Developmental Biology, Imperial College School of Medicine, London, United Kingdom
    Blood 98:2396-402. 2001
  9. ncbi BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood
    A Dzionek
    Miltenyi Biotec GmbH, Bergisch Gladbach, Germany AmCell Corporation, Sunnyvale, CA 94089, USA
    J Immunol 165:6037-46. 2000
  10. ncbi Adipose-derived stem cells for regenerative medicine
    Jeffrey M Gimble
    Stem Cell Biology Laboratory and Clinical Nutrition Research Unit, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA
    Circ Res 100:1249-60. 2007

Detail Information

Publications398 found, 100 shown here

  1. ncbi Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival
    Tyler J Curiel
    Tulane University Health Science Center, New Orleans, Louisiana 70112, USA
    Nat Med 10:942-9. 2004
    ..This specific recruitment of T(reg) cells represents a mechanism by which tumors may foster immune privilege. Thus, blocking T(reg) cell migration or function may help to defeat human cancer...
  2. ncbi Isolation of multipotent mesenchymal stem cells from umbilical cord blood
    Oscar K Lee
    Department of Orthopaedics and Traumatology, Veterans General Hospital Taipei, Taipei 11221, Taiwan
    Blood 103:1669-75. 2004
    ..In conclusion, umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical waste. It can serve as an alternative source of mesenchymal stem cells to bone marrow...
  3. pmc Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo
    S Gronthos
    Craniofacial and Skeletal Diseases Branch, National Institute on Dental Research, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:13625-30. 2000
    ..This study isolates postnatal human DPSCs that have the ability to form a dentin/pulp-like complex...
  4. ncbi Mesenchymal progenitor cells in human umbilical cord blood
    A Erices
    Unidad de Biologia Celular, INTA, Universidad de Chile, Santiago, Chile
    Br J Haematol 109:235-42. 2000
    ..Our results suggest that preterm, as compared with term, cord blood is richer in mesenchymal progenitors, similar to haematopoietic progenitors...
  5. ncbi Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood
    Wolfgang Wagner
    Department of Medicine V, University of Heidelberg, Heidelberg, Germany
    Exp Hematol 33:1402-16. 2005
    ..Whereas surface antigen markers have failed to precisely define this population, microarray analysis might provide a better tool for characterization of MSC...
  6. ncbi Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells
    Mauro Krampera
    Department of Clinical and Experimental Medicine, University of Verona, Italy
    Stem Cells 24:386-98. 2006
    ..These findings suggest that the beneficial effect on graft-versus-host disease induced by in vivo coinfusion with the graft of MSCs may be due to the activation of the immunomodulatory properties of MSCs by T cell- derived IFN-gamma...
  7. doi Neutrophils and granulocytic myeloid-derived suppressor cells: immunophenotyping, cell biology and clinical relevance in human oncology
    Claudia A Dumitru
    Department of Otorhinolaryngology, University of Duisburg Essen, Hufelandstrase 55, Essen, Germany
    Cancer Immunol Immunother 61:1155-67. 2012
    ..We propose a six-color immunophenotyping protocol that discriminates between monocytic MDSCs (MoMDSCs), two subsets of GrMDSCs and two subsets of ..
  8. ncbi Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow
    C Campagnoli
    Department of Maternal and Fetal Medicine, Institute of Reproductive and Developmental Biology, Imperial College School of Medicine, London, United Kingdom
    Blood 98:2396-402. 2001
    ..MSCs similar to those derived from adult bone marrow, fetal liver, and fetal bone marrow circulate in first-trimester human blood and may provide novel targets for in utero cellular and gene therapy...
  9. ncbi BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood
    A Dzionek
    Miltenyi Biotec GmbH, Bergisch Gladbach, Germany AmCell Corporation, Sunnyvale, CA 94089, USA
    J Immunol 165:6037-46. 2000
    ..The three presumably novel Ags serve as specific markers for the respective subpopulations of blood dendritic cells in fresh blood and will be of great value for their further analysis and to evaluate their therapeutic potential...
  10. ncbi Adipose-derived stem cells for regenerative medicine
    Jeffrey M Gimble
    Stem Cell Biology Laboratory and Clinical Nutrition Research Unit, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA
    Circ Res 100:1249-60. 2007
    ..The isolation, characterization, and preclinical and clinical application of adipose-derived stem cells (ASCs) are reviewed in this article...
  11. ncbi Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions
    H Jonuleit
    Department of Dermatology, University of Mainz, Germany
    Eur J Immunol 27:3135-42. 1997
    ..TNF-alpha/IL-1, IL-6 + PGE2-cultured DC seem to be optimal for generation of IFN-gamma-producing CD4/CD8+ T cells...
  12. pmc Lung environment determines unique phenotype of alveolar macrophages
    Amanda M Guth
    Departments of Clinical Sciences, Colorado State University, Ft Collins, USA
    Am J Physiol Lung Cell Mol Physiol 296:L936-46. 2009
    ....
  13. doi Human bone marrow and adipose tissue mesenchymal stem cells: a user's guide
    Federico Mosna
    Stem Cell Research Laboratory, Section of Hematology, Department of Medicine, Policlinico G B Rossi University of Verona, Verona, Italy
    Stem Cells Dev 19:1449-70. 2010
    ..We chose to use BM-MSCs as a model to describe techniques that have been used for MSC isolation and expansion from very different sources, and AT-MSCs as an example of a reliable and increasingly common alternative source...
  14. doi Report of the European Myeloma Network on multiparametric flow cytometry in multiple myeloma and related disorders
    Andy C Rawstron
    HMDS, Leeds Teaching Hospitals NHS Trust, Leeds, UK
    Haematologica 93:431-8. 2008
    ....
  15. pmc Aging of mesenchymal stem cell in vitro
    Mandana Mohyeddin Bonab
    Hematology Oncology and BMT Research Center, Shariati Hospital, Tehran University of Medical Sciences, Iran
    BMC Cell Biol 7:14. 2006
    ..The low frequency of this subpopulation of stem cells in bone marrow (BM) necessitates their in vitro expansion prior to clinical use. We evaluated the effect of long term culture on the senescence of these cells...
  16. ncbi Flow cytometric immunophenotypic analysis of 306 cases of multiple myeloma
    Pei Lin
    Department of Pathology and Laboratory Medicine, University of Arkansas for Medical Sciences, Little Rock, USA
    Am J Clin Pathol 121:482-8. 2004
    Bone marrow aspirates from 306 patients with multiple myeloma were analyzed by flow cytometric immunophenotyping. The plasma cells (PCs) were identified by their characteristic light scatter distribution and reactivity patterns to CD138, ..
  17. doi Clinical utility of microarray-based gene expression profiling in the diagnosis and subclassification of leukemia: report from the International Microarray Innovations in Leukemia Study Group
    Torsten Haferlach
    MLL Munich Leukemia Laboratory, Munich, Germany
    J Clin Oncol 28:2529-37. 2010
    ..The Microarray Innovations in Leukemia study assessed the clinical utility of gene expression profiling as a single test to subtype leukemias into conventional categories of myeloid and lymphoid malignancies...
  18. pmc Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3
    Wanjun Chen
    Cellular Immunology Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 198:1875-86. 2003
    ..Finally, in a murine asthma model, coadministration of these TGF-beta-induced suppressor T cells prevented house dust mite-induced allergic pathogenesis in lungs...
  19. ncbi Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood
    David A Ingram
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202, USA
    Blood 104:2752-60. 2004
    ....
  20. pmc Increased frequency and clinical significance of myeloid-derived suppressor cells in human colorectal carcinoma
    Hong Li Sun
    Department of Hematology, Wuxi People s Hospital, Wuxi 214023, Jiangsu Province, China
    World J Gastroenterol 18:3303-9. 2012
    ..To investigate the frequency and clinical significance of the myeloid-derived suppressor cells (MDSC) in human colorectal carcinoma (CRC)...
  21. pmc EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols
    T Kalina
    Department of Pediatric Hematology and Oncology, 2nd Faculty of Medicine, Charles University DPH O, Prague, Czech Republic
    Leukemia 26:1986-2010. 2012
    The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory ..
  22. doi Human mesenchymal stem cells: from immunophenotyping by flow cytometry to clinical applications
    Arthur A Nery
    Departamento de Bioquimica, Universidade de Sao Paulo, Sao Paulo, Brazil
    Cytometry A 83:48-61. 2013
    ..Large efforts, relying mostly on flow cytometry and imaging techniques, have been put into cell isolation, immunophenotyping, and studies of differentiation properties of stem cells of diverse origins...
  23. ncbi Essential role for ICSBP in the in vivo development of murine CD8alpha + dendritic cells
    Julio Aliberti
    Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 101:305-10. 2003
    ..Together these results demonstrate that ICSBP is critically required for the in vivo differentiation of CD8alpha(+) DCs and may also influence the functional maturation of the CD8alpha(-) subsets...
  24. ncbi Mucosal B cells: phenotypic characteristics, transcriptional regulation, and homing properties
    Per Brandtzaeg
    Laboratory for Immunohistochemistry and Immunopathology LIIPAT, Institute and Department of Pathology, University of Oslo, Rikshospitalet University Hospital, Oslo, Norway
    Immunol Rev 206:32-63. 2005
    ....
  25. pmc Homeostasis of peripheral B cells in the absence of B cell influx from the bone marrow
    Z Hao
    Department of Immunology, Institute for Genetics, University of Cologne, D 50931 Cologne, Germany
    J Exp Med 194:1151-64. 2001
    ..Mice in which RAG-2 was deleted at birth maintained a small population of activated B cells with an increased proportion of marginal zone B cells. Additionally, an increase of the pool of IgM secreting cells and B-1a cells was observed...
  26. doi A new population of myeloid-derived suppressor cells in hepatocellular carcinoma patients induces CD4(+)CD25(+)Foxp3(+) T cells
    Bastian Hoechst
    Medizinische Hochschule Hannover, Department of Gastroenterology, Hepatology and Endocrinology, Hannover, Germany
    Gastroenterology 135:234-43. 2008
    ..In this study, we have investigated the frequency and function of a new population of MDSC denoted here as CD14(+)HLA-DR(-/low) in HCC patients. We have also identified a novel, MDSC-mediated immune regulatory pathway in these patients...
  27. doi Differentiation of type 1 T regulatory cells (Tr1) by tolerogenic DC-10 requires the IL-10-dependent ILT4/HLA-G pathway
    Silvia Gregori
    Department of Regenerative Medicine, Stem Cells, and Gene Therapy, San Raffaele Telethon Institute for Gene Therapy, Via Olgettina 58, Milan, Italy
    Blood 116:935-44. 2010
    ..Our data indicate that DC-10 represents a novel subset of tolerogenic DCs, which secrete high levels of IL-10, express ILT4 and HLA-G, and have the specific function to induce Tr1 cells...
  28. ncbi Characterization of human blood dendritic cell subsets
    Kelli P A MacDonald
    Dendritic Cell Laboratory, Mater Medical Research Institute, Mater Misericordiae Hospitals, South Brisbane, Australia
    Blood 100:4512-20. 2002
    ..These data provide an opportunity to standardize the DC populations used for future molecular, functional and possibly even therapeutic studies...
  29. ncbi Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth
    C Morsczeck
    Stiftung Caesar, Center of Advanced European Studies and Research, Ludwig Erhard Allee 2, 53175 Bonn, Germany
    Matrix Biol 24:155-65. 2005
    ..Therefore, our results demonstrate that cultured PCs are unique undifferentiated lineage committed cells residing in the periodontium prior or during tooth eruption...
  30. ncbi Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications
    Christelle Doucet
    Département Recherches, centre de transfusion sanguine des armées Jean Julliard, Clamart Cedex, France
    J Cell Physiol 205:228-36. 2005
    ..Therefore, we propose that PL may be a powerful and safe substitute for FCS in development of tissue- and cellular-engineered products in clinical settings using MSCs...
  31. ncbi Expansion of mesenchymal stem cells isolated from pediatric and adult donor bone marrow
    Katia Mareschi
    Department of Pediatrics, Regina Margherita Children s Hospital, University of Turin, Turin, Italy
    J Cell Biochem 97:744-54. 2006
    ..No chromosomal alteration or evidence of cellular senescence were observed in all analyzed samples. All these data suggest that MSCs can be isolated and expanded from most healthy donors, providing for an autologous source of stem cells...
  32. ncbi [gamma][delta] cells: a right time and a right place for a conserved third way of protection
    A C Hayday
    Department of Immunobiology, Guy s King s St Thomas Medical School, King s College, University of London, United Kingdom
    Annu Rev Immunol 18:975-1026. 2000
    ..We conclude by considering which gammadelta cell functions may be most critical...
  33. ncbi Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies
    Nadia Quirici
    Fondazione Matarelli, Ospedale Fatebenefratelli e Oftalmico, Milan, Italy
    Exp Hematol 30:783-91. 2002
    ..We now show that LNGFR antibodies label primitive MSCs with high specificity and purity in adult BM, and compare these cells to those isolated by plastic adherence (PA) and CD45(-)anti-glycophorin A(-) selection...
  34. ncbi Increased production of immature myeloid cells in cancer patients: a mechanism of immunosuppression in cancer
    B Almand
    Department of Medicine and The Vanderbilt Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Immunol 166:678-89. 2001
    ..This may represent a new mechanism of immune suppression in cancer and may suggest a new approach to cancer treatment...
  35. ncbi Prevalence of regulatory T cells is increased in peripheral blood and tumor microenvironment of patients with pancreas or breast adenocarcinoma
    Udaya K Liyanage
    Alvin J Siteman Cancer Center, Department of Surgery, Washington University School of Medicine, St Louis, MO 63110, USA
    J Immunol 169:2756-61. 2002
    ..These T(reg) may mitigate the immune response against cancer, and may partly explain the poor immune response against tumor Ags...
  36. ncbi Mesenchymal stem cells reside in virtually all post-natal organs and tissues
    Lindolfo da Silva Meirelles
    Departamento de Genetica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
    J Cell Sci 119:2204-13. 2006
    ..These findings have implications for understanding MSC biology, and for clinical and pharmacological purposes...
  37. doi Flow cytometric immunophenotyping for hematologic neoplasms
    Fiona E Craig
    Division of Hematopathology, Department of Pathology, University of Pittsburgh School of Medicine, PA, USA
    Blood 111:3941-67. 2008
    Flow cytometric immunophenotyping remains an indispensable tool for the diagnosis, classification, staging, and monitoring of hematologic neoplasms...
  38. ncbi Immunogenicity of umbilical cord tissue derived cells
    Patricia S Cho
    Transplantation Biology Research Center, Massachusetts General Hospital, Boston, MA 02129, USA
    Blood 111:430-8. 2008
    ....
  39. pmc Immunophenotyping invasive breast cancer: paving the road for molecular imaging
    Jeroen F Vermeulen
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    BMC Cancer 12:240. 2012
    ..Because development of new tracers is labor-intensive and costly, we searched for the smallest panel of tumor membrane markers that would allow detection of the wide spectrum of invasive breast cancers...
  40. doi Distinct differentiation potential of "MSC" derived from cord blood and umbilical cord: are cord-derived cells true mesenchymal stromal cells?
    Julia Bosch
    Heinrich Heine University Medical Center, Institute for Transplantation Diagnostics and Cell Therapeutics, Duesseldorf, Germany
    Stem Cells Dev 21:1977-88. 2012
    ..The UC cells can be distinguished from CB- and BM-derived cells as well as from pericytes and foreskin fibroblasts by the expression of HOX-genes and the cell surface antigens CD56 and CD146...
  41. pmc Immunophenotyping in post-giardiasis functional gastrointestinal disease and chronic fatigue syndrome
    Kurt Hanevik
    Institute of Medicine, University of Bergen, Bergen N 5021, Norway
    BMC Infect Dis 12:258. 2012
    ..The aim of this study was to evaluate a wide selection of markers of immune dysfunction in these two co-occurring post-infectious syndromes...
  42. doi Prognostic value of immunophenotyping in multiple myeloma: a study by the PETHEMA/GEM cooperative study groups on patients uniformly treated with high-dose therapy
    Gema Mateo
    Hospital Universitario de Salamanca, Salamanca, Spain
    J Clin Oncol 26:2737-44. 2008
    To analyze the prognostic impact of immunophenotyping in patients with multiple myeloma (MM).
  43. pmc Induction of interleukin 10-producing, nonproliferating CD4(+) T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells
    H Jonuleit
    Department of Dermatology, University of Mainz, D 55101 Mainz, Germany
    J Exp Med 192:1213-22. 2000
    ..These data show that immature and mature DCs induce different types of T cell responses: inflammatory Th1 cells are induced by mature DCs, and IL-10-producing T cell regulatory 1-like cells by immature DCs...
  44. doi Utility of flow cytometry immunophenotyping in multiple myeloma and other clonal plasma cell-related disorders
    Bruno Paiva
    Department of Medicine, Services of Cytometry and Hematology and Cancer Research Center CIC, IBMCC USAL CSIC, University of Salamanca and University Hospital of Salamanca, Salamanca, Spain
    Cytometry B Clin Cytom 78:239-52. 2010
    In recent years, multiparameter flow cytometry (MFC) immunophenotyping has become mandatory in the clinical management of hematological malignancies, both for diagnostic and monitoring purposes...
  45. pmc Ex vivo isolation and characterization of CD4(+)CD25(+) T cells with regulatory properties from human blood
    D Dieckmann
    Department of Dermatology, University of Erlangen-Nuremberg, Hartmannstrasse 14, 91052 Erlangen, Germany
    J Exp Med 193:1303-10. 2001
    ..The identification of regulatory CD4(+)CD25(+) T cells has important implications for the study of tolerance in man, notably in the context of autoimmunity, transplantation, and cancer...
  46. pmc Human immunodeficiency virus type 1 (HIV-1)-specific CD8+ T(EMRA) cells in early infection are linked to control of HIV-1 viremia and predict the subsequent viral load set point
    John W Northfield
    Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, South Parks Road, Oxford OX1 3SY, United Kingdom
    J Virol 81:5759-65. 2007
    ....
  47. pmc Development of promyelocytic zinc finger and ThPOK-expressing innate gamma delta T cells is controlled by strength of TCR signaling and Id3
    ERIC S ALONZO
    Immunology Program, Sloan Kettering Institute, New York, NY, 10065, USA
    J Immunol 184:1268-79. 2010
    ..This study defines the factors that control the propensity of the immune system to produce potentially disease-causing T cell subsets...
  48. pmc Characterization of cytokine-induced myeloid-derived suppressor cells from normal human peripheral blood mononuclear cells
    Melissa G Lechner
    Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    J Immunol 185:2273-84. 2010
    ..Suppressive CD33(+) cells generated from PBMCs by GM-CSF and IL-6 were consistent with human MDSCs. This study suggests that these cytokines are potential therapeutic targets for the inhibition of MDSC induction in cancer patients...
  49. ncbi Surface protein characterization of human adipose tissue-derived stromal cells
    S Gronthos
    Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, Bethesda, Maryland, USA
    J Cell Physiol 189:54-63. 2001
    ..The similarities between the phenotypes of human adipose tissue-derived and human bone marrow-derived stromal cells could have broad implications for human tissue engineering...
  50. pmc In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties
    Christoph le Viseur
    Department of Pediatric Hematology and Oncology, University Children s Hospital Munster, 48129 Munster, Germany
    Cancer Cell 14:47-58. 2008
    ..Sorted blast populations mirrored normal B precursor cells with transcription of a number of stage-appropriate genes. These observations inform a model for leukemia-propagating stem cells in childhood ALL...
  51. ncbi No alloantibodies against mesenchymal stromal cells, but presence of anti-fetal calf serum antibodies, after transplantation in allogeneic hematopoietic stem cell recipients
    Mikael Sundin
    Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Haematologica 92:1208-15. 2007
    ..The objective of this study was to investigate whether antibodies against MSC were present after hematopoietic stem cell transplantation (HSCT) including treatment with matched or mismatched allogeneic MSC...
  52. ncbi T cell responses to allogeneic human mesenchymal stem cells: immunogenicity, tolerance, and suppression
    Elena Klyushnenkova
    Osiris Therapeutics, Inc, 2001 Aliceanna Street, Baltimore, MD 21231, USA
    J Biomed Sci 12:47-57. 2005
    ..We conclude that MSCs can initiate activation of alloreactive T cells, but do not elicit T cell proliferative responses due to active suppressive mechanisms...
  53. ncbi Functional heterogeneity of memory CD4 T cell responses in different conditions of antigen exposure and persistence
    Alexandre Harari
    Laboratory of AIDS Immunopathogenesis, Division of Immunology and Allergy, Department of Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    J Immunol 174:1037-45. 2005
    ..The results obtained supported the hypothesis that the different patterns observed were substantially influenced by different conditions of Ag exposure and persistence...
  54. ncbi Immunophenotyping of leukemias using a cluster of differentiation antibody microarray
    L Belov
    Department of Biochemistry, Institute for Biomedical Research, University of Sydney, NSW 2006, Australia
    Cancer Res 61:4483-9. 2001
    ..The extent of use of CD antigen expression (immunophenotyping) for identification of leukemias has been constrained by the technique used, flow cytometry, which commonly ..
  55. ncbi Outgrowth of a transformed cell population derived from normal human BM mesenchymal stem cell culture
    Y Wang
    Division of Hematologic Malignancies, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cytotherapy 7:509-19. 2005
    ..Human mesenchymal stem cells (hMSC) have been isolated and characterized extensively for a variety of clinical applications. Yet it is unclear how the phenomenon of hMSC plasticity can be safely and reasonably exploited for therapeutic use...
  56. ncbi Diffuse large B-cell lymphomas with plasmablastic differentiation represent a heterogeneous group of disease entities
    Lluis Colomo
    Hematopathology Section, Laboratory of Pathology, and Department of Hematology, Hospital Clinic, Institut d Investigacions Biomediques August Pi i Sunyer, University of Barcelona, Barcelona, Spain
    Am J Surg Pathol 28:736-47. 2004
    ..In conclusion, DLBCLs with plasmablastic differentiation are a heterogeneous group of neoplasms with different clinicopathological characteristics that may correspond to different entities...
  57. pmc Compromised CD4+ CD25(high) regulatory T-cell function in patients with relapsing-remitting multiple sclerosis is correlated with a reduced frequency of FOXP3-positive cells and reduced FOXP3 expression at the single-cell level
    Koen Venken
    Hasselt University, Biomedisch Onderzoeksinstituut and Transnationale Universiteit Limburg, School of Life Sciences, Diepenbeek, Belgium
    Immunology 123:79-89. 2008
    ..Our results also suggest that Tregs accumulate in the CSF of RR-MS patients, in an attempt to down-regulate local inflammation in the central nervous system...
  58. pmc Cytomegalovirus infection in Gambian infants leads to profound CD8 T-cell differentiation
    David J C Miles
    MRC Laboratories Gambia, P O Box 273, Banjul, The Gambia
    J Virol 81:5766-76. 2007
    ....
  59. pmc Developmental stage, phenotype, and migration distinguish naive- and effector/memory-like CD4+ regulatory T cells
    Jochen Huehn
    Experimentelle Rheumatologie, Medizinische Klinik, Charite, Humboldt Universitaet, Schumannstr 21 22, 10117 Berlin, Germany
    J Exp Med 199:303-13. 2004
    ..Accordingly, alphaE -expressing cells were found to be the most potent suppressors of inflammatory processes in disease models such as antigen-induced arthritis...
  60. ncbi IFNgamma and lymphocytes prevent primary tumour development and shape tumour immunogenicity
    V Shankaran
    Department of Pathology and Immunology, Center for Immunology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, Missouri 63110, USA
    Nature 410:1107-11. 2001
    ..However, this process also leads to the immunoselection of tumour cells that are more capable of surviving in an immunocompetent host, which explains the apparent paradox of tumour formation in immunologically intact individuals...
  61. pmc Similar MLL-associated leukemias arising from self-renewing stem cells and short-lived myeloid progenitors
    Antonio Cozzio
    Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Genes Dev 17:3029-35. 2003
    ....
  62. ncbi Enumeration and phenotypic characterization of synovial fluid multipotential mesenchymal progenitor cells in inflammatory and degenerative arthritis
    Elena A Jones
    University of Leeds, Leeds, UK
    Arthritis Rheum 50:817-27. 2004
    ..To evaluate synovial fluid (SF) for the presence of mesenchymal progenitor cells (MPCs), to compare SF MPCs with bone marrow (BM) MPCs, and to enumerate these cells in both inflammatory arthritis and osteoarthritis (OA)...
  63. pmc Perforin and IL-2 upregulation define qualitative differences among highly functional virus-specific human CD8 T cells
    George Makedonas
    Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS Pathog 6:e1000798. 2010
    ....
  64. ncbi Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine
    Hiromasa Hamada
    Department of Microbiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Immunol 168:57-64. 2002
    ..These findings indicate that ILF, PP, and CP constitute three distinct organized gut-associated lymphoid tissues that reside in the lamina propria of the mouse small intestine...
  65. ncbi Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis
    Koichiro Iohara
    Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi, Japan
    Stem Cells 24:2493-503. 2006
    ..Thus, adult pulp contains SP cells, which are enriched for stem cell properties and useful for cell therapy with BMP2 for dentin regeneration...
  66. pmc Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases
    Zaruhi Hovhannisyan
    Immunology Institute, Mount Sinai School of Medicine, New York, New York 10029, USA
    Gastroenterology 140:957-65. 2011
    ..We studied Th17 and Treg cells isolated from intestinal lamina propria of patients with IBD to investigate their role in pathogenesis...
  67. ncbi Lymphoid neogenesis in rheumatoid synovitis
    S Takemura
    Department of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    J Immunol 167:1072-80. 2001
    ....
  68. doi ALK- anaplastic large-cell lymphoma is clinically and immunophenotypically different from both ALK+ ALCL and peripheral T-cell lymphoma, not otherwise specified: report from the International Peripheral T-Cell Lymphoma Project
    Kerry J Savage
    Department of Medical Oncology, British Columbia Cancer Agency, Vancouver, BC, Canada
    Blood 111:5496-504. 2008
    ..Although the prognosis of ALK(-) ALCL appears to be better than that for PTCL-NOS, it is still unsatisfactory and better therapies are needed. Primary cutaneous ALCL is associated with an indolent course...
  69. ncbi MyD88-dependent activation of dendritic cells and CD4(+) T lymphocytes mediates symptoms, but is not required for the immunological control of parasites during rodent malaria
    Bernardo S Franklin
    Centro de Pesquisas Rene Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil
    Microbes Infect 9:881-90. 2007
    ..chabaudi...
  70. ncbi Allogeneic marrow stromal cells are immune rejected by MHC class I- and class II-mismatched recipient mice
    Nicoletta Eliopoulos
    Lady Davis Institute for Medical Research, McGill University, 3755 Cote St Catherine Rd, Montreal, QC Canada H3T1E2
    Blood 106:4057-65. 2005
    ..These results strongly suggest that MSCs are not intrinsically immunoprivileged and cannot serve as a "universal donor" in immunocompetent MHC-mismatched recipients...
  71. ncbi Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue
    Susanne Kern
    Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden Württemberg Hessen, Mannheim, Germany
    Stem Cells 24:1294-301. 2006
    ..Both UCB and AT are attractive alternatives to BM in isolating MSC: AT as it contains MSCs at the highest frequency and UCB as it seems to be expandable to higher numbers...
  72. pmc Long-term outcome of EBV-specific T-cell infusions to prevent or treat EBV-related lymphoproliferative disease in transplant recipients
    Helen E Heslop
    Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA
    Blood 115:925-35. 2010
    ....
  73. ncbi Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation
    Pieternella S In 't Anker
    Blood 102:1548-9. 2003
  74. doi Immunophenotypic identification and characterization of tumor cells and infiltrating cell populations in meningiomas
    Patrícia H Domingues
    Centre for Neurosciences and Cell Biology, Faculty of Pharmacy, University of Coimbra, Portugal
    Am J Pathol 181:1749-61. 2012
    ..We determined the cellular composition of 51 meningioma samples by multiparameter flow cytometric (MFC) immunophenotyping and investigated the potential relationship between mRNA and protein expression levels of neoplastic cells...
  75. doi Cellometer vision as an alternative to flow cytometry for cell cycle analysis, mitochondrial potential, and immunophenotyping
    Leo L Chan
    Department of Technology R and D, Nexcelom Bioscience LLC, Lawrence, Massachusetts 01843, USA
    Cytometry A 79:507-17. 2011
    ..Cell population analysis assays such as immunophenotyping, cell cycle, and mitochondrial membrane potential detection methods have not yet been reported for the ..
  76. pmc T cell subset-specific susceptibility to aging
    Marta Czesnikiewicz-Guzik
    Kathleen B and Mason I Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA30322, USA
    Clin Immunol 127:107-18. 2008
    ..The data show that CD8 T cells are age sensitive by at least two partially independent mechanisms: fragile homeostatic control and gene expression instability in a large set of regulatory cell surface molecules...
  77. ncbi Seventeen-colour flow cytometry: unravelling the immune system
    Stephen P Perfetto
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5507, Bethesda, Maryland 20892 3015, United States
    Nat Rev Immunol 4:648-55. 2004
  78. doi Markers of stemness in equine mesenchymal stem cells: a plea for uniformity
    Catharina De Schauwer
    Department of Reproduction, Obstetrics and Herd Health, Ghent University, Merelbeke, Belgium
    Theriogenology 75:1431-43. 2011
    ..However, their unequivocal immunophenotyping is hampered by the lack of a single specific marker and the limited availability of monoclonal anti-horse ..
  79. pmc Subspecialization of CXCR5+ T cells: B helper activity is focused in a germinal center-localized subset of CXCR5+ T cells
    C H Kim
    Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Exp Med 193:1373-81. 2001
    ..Our results demonstrate that CXCR5+ T cells are functionally heterogeneous and that the GC-Th cells, a small subset of CXCR5+ T cells, are the key helpers for B cell differentiation and antibody production in lymphoid tissues...
  80. ncbi Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire
    J J Campbell
    Joint Program in Transfusion Medicine, Children s Hospital, 300 Longwood Avenue, Room BD 401, Boston, MA 02115
    J Immunol 166:6477-82. 2001
    ..These findings describe a diverse population of cells that may have trafficking patterns entirely different from each other, and from other lymphocyte types...
  81. pmc Immunophenotyping of lymphocyte, monocyte and dendritic cell subsets in normal rhesus macaques by 12-color flow cytometry: clarification on DC heterogeneity
    Patrick Autissier
    Department of Biology, Boston College, Higgins Hall 468, 140 Commonwealth Avenue, Chestnut Hill, MA 02467, USA
    J Immunol Methods 360:119-28. 2010
    ....
  82. ncbi Reference values for peripheral blood lymphocyte phenotypes applicable to the healthy adult population in Switzerland
    Leslie R Bisset
    Department of Internal Medicine, University Hospital, Zurich, Switzerland
    Eur J Haematol 72:203-12. 2004
    ....
  83. ncbi Dynamics of macrophage cell populations during murine pulmonary tuberculosis
    Mercedes Gonzalez-Juarrero
    Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    J Immunol 171:3128-35. 2003
    ..This analytical approach may facilitate the further characterization of macrophage populations entering into the lung tissues and their relative contributions to host resistance to tuberculosis infection...
  84. doi Generation of highly suppressive adaptive CD8(+)CD25(+)FOXP3(+) regulatory T cells by continuous antigen stimulation
    Milada Mahic
    Biotechnology Centre and Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo, Norway
    Eur J Immunol 38:640-6. 2008
    ..However, neither blocking antibodies to CTLA-4, CD80 nor CD86 could reverse CD8(+) T(R)-mediated suppression, indicating that other mechanism(s) must be employed by these cells...
  85. ncbi Immunophenotypic analysis of Waldenstrom's macroglobulinemia
    J F San Miguel
    Servicio de Hematologia, Hospital Universitario de Salamanca, Spain
    Semin Oncol 30:187-95. 2003
    b>Immunophenotyping has become an essential tool for diagnosis of hematological malignancies. By contrast, for diagnosis of Waldenstrom's macroglobulinemia (WM) immunophenotyping is used only occasionally...
  86. doi AML with mutated NPM1 carrying a normal or aberrant karyotype show overlapping biologic, pathologic, immunophenotypic, and prognostic features
    Claudia Haferlach
    Munich Leukemia Laboratory GmbH, Munich, Germany
    Blood 114:3024-32. 2009
    ....
  87. pmc Circulating human B and plasma cells. Age-associated changes in counts and detailed characterization of circulating normal CD138- and CD138+ plasma cells
    Anouk Caraux
    INSERM, U847, Montpellier, France
    Haematologica 95:1016-20. 2010
    ..Unlike B lymphocytes, both populations of plasma cells are KI-67(+) and show weak CXCR4 expression...
  88. doi Expansion of adipose mesenchymal stromal cells is affected by human platelet lysate and plating density
    Dominik Cholewa
    Stem Cell Biology and Cellular Engineering, Medical Faculty, RWTH Aachen University, Aachen, Germany
    Cell Transplant 20:1409-22. 2011
    ..Cellular automaton computer simulations provided additional insights into how complex population dynamics during long-term expansion are affected by plating density and migration...
  89. doi The utility and limitations of neurosphere assay, CD133 immunophenotyping and side population assay in glioma stem cell research
    Feng Wan
    Department of Neurosurgery, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China
    Brain Pathol 20:877-89. 2010
    ..review the current widely used methods for enriching and isolating GSCs, namely neurosphere assay, CD133 Immunophenotyping and side population assay, and detail their limitations and potential pitfalls that could complicate ..
  90. doi Highly multiparametric analysis by mass cytometry
    Olga Ornatsky
    Department of Chemistry, University of Toronto, 80 St George St, Toronto ON, Canada M5S 3H6 DVS Sviences Inc, 70 Peninsula Cr, Richmond Hill, ON L4S1Z5, Canada
    J Immunol Methods 361:1-20. 2010
    ..Here we describe mass cytometric immunophenotyping of human leukemia cell lines and leukemia patient samples, differential cell analysis of normal peripheral ..
  91. pmc Patient-centred screening for primary immunodeficiency, a multi-stage diagnostic protocol designed for non-immunologists: 2011 update
    E de Vries
    Department of Paediatrics, Jeroen Bosch Hospital, s Hertogenbosch, The Netherlands
    Clin Exp Immunol 167:108-19. 2012
    ....
  92. doi Flow cytometry immunophenotyping of fine-needle aspiration specimens: utility in the diagnosis and classification of non-Hodgkin lymphomas
    Susana Barrena
    Servicio General de Citometria, Departamento de Medicina and Centro de Investigación del Cáncer IBMCC CSIC USAL, Universidad de Salamanca, Salamanca, Spain
    Histopathology 58:906-18. 2011
    ..To establish the utility of flow cytometry (FCM) for screening and diagnosis of B cell non-Hodgkin lymphoma (B-NHL) from lymphoid tissue samples obtained by fine-needle aspiration (FNA)...
  93. doi Review of phenotypic markers used in flow cytometric analysis of MGUS and MM, and applicability of flow cytometry in other plasma cell disorders
    Karthick R M Raja
    University Research Centre Czech Myeloma Group, Kamenice 5, Bohunice, Brno 62500, Czech Republic
    Br J Haematol 149:334-51. 2010
    Flow cytometric immunophenotyping is considered an indispensable tool for the diagnosis, classification and monitoring of disease in monoclonal gammopathies...
  94. ncbi CCR7 expression and memory T cell diversity in humans
    J J Campbell
    Joint Program in Transfusion Medicine, Children s Hospital, and Harvard Medical School, Department of Pathology, Boston, MA 02115, USA
    J Immunol 166:877-84. 2001
    ..The ratio of CD27(neg)/CCR7(neg) cells to CD27(pos)/CCR7(pos) cells varies from tissue to tissue, and may correlate with the number of cells actively engaged in Ag recognition within a given tissue...
  95. pmc Differential expression of HIF-1α in CD44+CD24-/low breast ductal carcinomas
    Joao Paulo Oliveira-Costa
    Department of Pathology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
    Diagn Pathol 6:73. 2011
    ..There are evidence that hypoxia could drive the maintenance of CSC, via HIF-1α expression, but it still to be determined whether hypoxia markers are expressed in breast tumors presenting CD44+CD24-/low immunophenotype...
  96. doi A role for immature myeloid cells in immune senescence
    Elena Y Enioutina
    Department of Pathology, University of Utah, Salt Lake City, UT 84132, USA
    J Immunol 186:697-707. 2011
    ..Our data demonstrate that abnormal activities of the Gr1(+)CD11b(+) myeloid cell population from aged mice could play a significant role in the mechanisms responsible for immune senescence...
  97. doi Clinical-grade, large-scale, feeder-free expansion of highly active human natural killer cells for adoptive immunotherapy using an automated bioreactor
    Tolga Sutlu
    Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden
    Cytotherapy 12:1044-55. 2010
    ..The goal of this study was to optimize a large-scale, feeder-free, closed system for efficient NK cell expansion...
  98. pmc Evaluation of a simplified dual-platform flow cytometric method for measurement of lymphocyte subsets and T-cell maturation phenotypes in the population of Nouna, Burkina Faso
    T Bohler
    Department of Virology, University of Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Clin Vaccine Immunol 14:775-81. 2007
    ..Among CD8(bright) lymphocytes, 3% +/- 2% had a central memory phenotype, 27% +/- 13% were naïve, 37% +/- 13% had an effector memory phenotype, and 34% +/- 12% were terminally differentiated effector memory cells expressing CD45RA...
  99. ncbi Differential impact of metacyclic and blood trypomastigotes on parasitological, serological and phenotypic features triggered during acute Trypanosoma cruzi infection in dogs
    Claudia Martins Carneiro
    Departamento de Analises Clinicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil
    Acta Trop 101:120-9. 2007
    ..These findings emphasise the importance of inoculum source and suggest that vectorial or transfusional routes of T. cruzi infection may trigger distinct parasite-host interactions during acute Chagas disease...
  100. pmc Regulatory T cells phenotype in different clinical forms of Chagas' disease
    Fernanda Fortes de Araújo
    Laboratorio de Imunologia Celular e Molecular, Centro de Pesquisas Rene Rachou, FIOCRUZ, Belo Horizonte, Brasil
    PLoS Negl Trop Dis 5:e992. 2011
    ..Ex vivo immunophenotyping of whole blood, as well as after stimulation with Trypanosoma cruzi antigens, demonstrated that individuals ..
  101. doi Natural-killer cell amplification for adoptive leukemia relapse immunotherapy: comparison of three cytokines, IL-2, IL-15, or IL-7 and impact on NKG2D, KIR2DL1, and KIR2DL2 expression
    Véronique Decot
    Unité de Thérapie cellulaire et Tissus, CHU Nancy, Nancy, France
    Exp Hematol 38:351-62. 2010
    ....

Research Grants62

  1. The Role of CRACM1 in the Pathogenesis of Systemic Lupus Erythematosus
    Peter C Chien; Fiscal Year: 2013
    ..b>Immunophenotyping will also determine which subpopulations of T cells may be expressing CRACM1...
  2. Fanconi Anemia as a Model for Susceptibility to Human Papillomavirus Infection
    MELINDA SUE BUTSCHKOVACIC; Fiscal Year: 2013
    ..Brazil, established relationships with the Fanconi Anemia Research Fund, and proven expertise in HPV typing, immunophenotyping and epidemiological analyses...
  3. Investigating gene x environment interaction using human exposures to O3 & LPS
    David B Peden; Fiscal Year: 2010
    ..The response phenotypes we will examine include lung function, airway inflammation, immunophenotyping of airway and circulating monocytes, macrophages and dendritic cells, airway and circulating phagocyte ..
  4. Obesity, Inflammation, and Lung Injury After Lung Transplantation
    David J Lederer; Fiscal Year: 2013
    ..of intrathoracic, visceral, and subcutaneous adipose tissue mass using quantitative CT imaging, (2) immunophenotyping of macrophages and T cells from intrathoracic adipose tissue and lymph nodes obtained immediately before and ..
  5. Gene Expression and Regulatory Networks in Human Leukocytes
    Diane J Mathis; Fiscal Year: 2010
    ..Using high-throughput approaches for genotyping, immunophenotyping and gene expression analysis, the project will examine the basis for the control of gene expression in human ..
  6. Novel Mucosa-homing Dendritic Cell: Development, Trafficking and Function
    Eugene C Butcher; Fiscal Year: 2013
    ..and other DC progenitors will be sorted from BM, and their development into DC subsets will be monitored by immunophenotyping after in vitro culture...
  7. Rapid Label-Free Detection of Acute Promyelocytic Leukemia
    Lydia L Sohn; Fiscal Year: 2012
    ..Unfortunately, APL diagnosis requires expert hematopathologic review and careful immunophenotyping. When APL patients present late at night or on the weekends to their local doctor or emergency room (ER), ..
  8. Genome-wide genetic variation in the human immune response to influenza vaccines
    LUIS MIGUEL FRANCO; Fiscal Year: 2013
    ..against influenza and a combination of genome-wide genotyping, global gene expression measurements and immunophenotyping to dissect the DNA variants that influence the human immune response to the vaccine...
  9. Effects of Acitretin on the activation of latent HIV
    Peilin Li; Fiscal Year: 2013
    ..but does not induce polyclonal activation of CD4 T cells as measured by gene expression and cell surface immunophenotyping for markers of T cell activation...
  10. Molecular Pathogenesis of Monoclonal B Cell Lymphocytosis and CLL
    Mark C Lanasa; Fiscal Year: 2013
    ..molecular biological analysis of MBL cases including single cell immunoglobulin gene analysis and detailed immunophenotyping;(ii) to explore BCR dependent signaling pathways in CLL and MBL and (iii) to follow a cohort of family-..
  11. Ancillary Studies in Clinical Trials - PRIMeR
    Marcelo C Pasquini; Fiscal Year: 2013
    ..S. transplant centers and started accrual in June of 2010. The PRIMeR (PRognostic Immunophenotyping in Myeloma Response) ancillary study will use a comprehensive 7-color panel of plasma cell markers to ..
  12. Immunologic and Molecular Phenotypes in AATD and Sarcoidosis
    Lisa A Maier; Fiscal Year: 2013
    ..Our investigators have expertise in the scientific focus of the RFA, including genomics, immunology/immunophenotyping, and clinically based research, which would serve as a resource for this U01 grant...
  13. Newborn Screening for T-Cell Disorders: Spectrum and Outcomes
    Jennifer M Puck; Fiscal Year: 2013
    ..By combining immunophenotyping, deep sequencing and genomic analysis with in vitro studies we will find new disease genes and more fully ..
  14. Hematopoietic Reconstitution Using Ex Vivo Expanded Cord Blood CD34+ Cells
    Gerald J Spangrude; Fiscal Year: 2012
    ..mouse model and the level of engraftment for each test groups will be evaluated using immunophenotyping/flow cytometry and Southern blotting (if necessary)...
  15. Biobehavioral Mechanisms Facilitating Recovery From Stem Cell Transplantation
    Erin Costanzo; Fiscal Year: 2013
    ..The first study of autologous transplant recipients will utilize novel multicolor flow cytometry and immunophenotyping techniques to delineate leukocyte reconstitution following transplant...
  16. Impact of malnutrition on TB-IRIS and pharmacokinetics of TB and ARV co-treatment
    ANNELIES T A VAN RIE; Fiscal Year: 2012
    ..the immunopathogenesis of TB-IRIS, with a focus on cytokine expression measurements, lymphocyte subset immunophenotyping and assessment of the role of regulatory T cells...
  17. ImageStream X Analyzer 2011
    FREDERIC IRA PREFFER; Fiscal Year: 2012
    ..By combining the speed, sensitivity, and immunophenotyping abilities and capacities of flow cytometry with the detailed imagery and functional insights of microscopy, ..
  18. TGFbeta Blockade in MART TCR-Engineered T Cell Melanoma Immunotherapy in Man
    Richard C Koya; Fiscal Year: 2013
    ..time points, and analyze peripheral blood and tumor infiltrating lymphocytes from biopsies for number, immunophenotyping, and functional assays...
  19. Science in a Culture of Mentoring
    Judith A James; Fiscal Year: 2011
    ..The Immunophenotyping Core applies new whole blood multiparameter testing of detailed subsets of B cells, T cells, and other cell ..
  20. Immunophenotyping and Lymphocyte Effector Functions in Sarcoidosis
    Laura L Koth; Fiscal Year: 2010
    ..abstract_text> ..
  21. Science in a Culture of Mentoring
    Judith A James; Fiscal Year: 2013
    ..The Immunophenotyping Core applies new whole blood multiparameter testing of detailed subsets of B cells, T cells, and other cell ..
  22. Genomic Phenotyping and Mechanisms in sarcoidosis and AAT
    Laura L Koth; Fiscal Year: 2013
    ..Illinois at Chicago (UIC) centers have joined together in this application to present genomic, genetic and immunophenotyping data which demonstrate clinical and experimental expertise as well as novel hypotheses related to ..
  23. Midcareer Award in Translational Immunology of Aging
    Albert C Shaw; Fiscal Year: 2013
    ..In the second, he is the PI of an R01 within a U19 Center Grant that is part of the NIH Human Immunophenotyping Consortium...
  24. Elucidating the Pathology of Impact-Induced Traumatic Brain Injury
    CHAD ALAN TAGGE; Fiscal Year: 2013
    ..Comprehensive neuropathological analysis and immunophenotyping will be used to characterize and localize neuroinflammation and focal tauopathy...
  25. A Novel Expansion of Primary Leukemia Stem Cells
    Ramasamy Sakthivel; Fiscal Year: 2010
    ..b>Immunophenotyping, CFU assay, and cytogenic analysis will be performed to measure culture clonogenic potential and phenotype/..
  26. Streamlined Banking of Human Pluripotent Stem Cells Through Molecular Authenticat
    Jonathan M Auerbach; Fiscal Year: 2010
    ..the new assays by comparing the results with those obtained by traditional tests being used now - including immunophenotyping and G-band karyotyping...
  27. Columbia University Medical Center Skin Disease Research Center (CUMCSDRC)
    DAVID RINSEY BICKERS; Fiscal Year: 2013
    ..These include: i) Tissue Culture and Histology Core;ii) Immunophenotyping Core;iii) Advanced Imaging Core;and iv) Administrative Core...
  28. High-speed cell sorter
    Ronald Veazey; Fiscal Year: 2002
    ..will allow TRPRC researchers to perform high speed cell sorting, calcium flux determination, and 6-color immunophenotyping. This equipment will be of tremendous assistance in accomplishing the aims of several currently-funded NIH-..
  29. HIV-1 Specific Cytotoxic T Lymphocytes in Breastmilk
    Grace John Stewart; Fiscal Year: 2003
    ..Paired blood and breast milk specimens at 2 and 4 weeks postpartum will be assessed for viral load, immunophenotyping, and lymphocyte responses to vv-env, vv-gag, and immunodominant peptides identified at delivery...
  30. ALLOGENEIC LEUKOCYTE INFUSIONS FOR BREAST CANCER
    SCOTT BEARMAN; Fiscal Year: 2000
    ..leukocytes, marrow and progenitor cells, (2) evaluation of immunocompetence pre- and post-infusion by both immunophenotyping peripheral blood leukocytes and by specific immune function studies, (3) evaluation of graft-versus-tumor ..
  31. Nostalgia in the WNT signaling pathway; fatty acids, epigenetics and leukemia
    Vincent Sollars; Fiscal Year: 2009
    ..b>Immunophenotyping with flow cytometry will be employed to follow cellular differentiation...
  32. HTLV-I INFECTION AND ATL
    Miguel Suarez; Fiscal Year: 1992
    ..The study will be conducted by instituting an immunophenotyping program of lymphoma-leukemia and through seroepidemiological approaches...
  33. CELL DEATH IN THE OLIGODENDROGLIAL LINEAGE
    Judith Grinspan; Fiscal Year: 1999
    ..They will utilize an assay which detects nuclear fragmentation, can be combined with immunophenotyping, and works well both in vitro and in vivo. Their specific aims are: 1...
  34. A Novel Model of TMJ Osteoarthritis to Define Glial Reactivity in Chronic Pain
    Beth Winkelstein; Fiscal Year: 2007
    ..which produces TMJ osteoarthritic lesions and persistent behavioral sensitivity; and (2) utilize immunophenotyping and cell sorting techniques to separate glial and neuronal cell populations, and real-time PCR to probe ..
  35. Cancer Study by Flow Cytometric Signal Cell Genotyping
    Hong Cai; Fiscal Year: 2006
    ..In hematopoietic malignances, flow cytometry-based immunophenotyping and polymerase chain reaction (PCR or Reverse Transcriptase, RT-PCR)-based sequence identification are the ..
  36. IMMUNE RESPONSE IN THE LUNG TO TB PROTEINS IN AIDS
    William Rom; Fiscal Year: 2002
    ..evaluate their clinical status and study their BAL cells and peripheral blood mononuclear cells (PBMCs) by immunophenotyping of lymphocyte subsets and by proliferation, IFN-gamma release, and CTL assays to assess their in vitro ..
  37. GENETIC MANAGEMENT OF CHIMPANZEE BREEDING AND RESEARCH
    John Ely; Fiscal Year: 1999
    ..We will utilize these pre-existing data in a collaboration involving DNA typing, quantitative genetics, and immunophenotyping to investigate the genetic epidemiology of this infectious disease and thereby fully exploit the research ..
  38. Mesenchymal Progenitors of Intragraft Fibroblast
    GORDON WU; Fiscal Year: 2003
    ..hierarchy of the fibroblastic lineage of MSC will be determined using MSC differentiation cultures, immunophenotyping and fibroblast functional assays...
  39. Multiplex Analysis of AIDS by Laser Scanning Cytometry
    Paul Luciw; Fiscal Year: 2003
    ..of biopsy and hematological specimens, analysis of fluorescence in situ hybridization, as well as combined immunophenotyping and DNA/RNA content analysis...
  40. Isolation of human tumor cells in dormant cancer
    Jonathan Uhr; Fiscal Year: 2007
    ..40 of these patients indicate that 18 have CTCs, using very stringent criteria including cytomorphology, immunophenotyping and aneusomy. Nineteen normal women had no cells in the blood that met the criteria for CTCs...
  41. Hematopoietic Circadian Rhythms
    Peter Quesenberry; Fiscal Year: 2005
    ..with availability of Per1-luc purified rat stem cells, we will study gene and protein expression using immunophenotyping and array technology. These experiments will employ congenic Ly-5.1 to Ly-5...
  42. CRYOPRESERVATION OF EJACULATED AND EPIDIDYMAL SPERM
    Alan Trounson; Fiscal Year: 2001
    ..the highest possible rates of liveborn pups; and (4) evaluate the normality of liveborn mice from (3) using immunophenotyping; histopathology; behavioural gene expression (using microarray and/or SAGE analysis) techniques...
  43. IMMUNOBIOLOGY OF NORMAL AND LEUKEMIC B-CELL PRECURSORS
    Fatih Uckun; Fiscal Year: 1992
    ..In vitro differentiation will be monitored by immunophenotyping and in the case of fetal liver cell lines also by Southern blot hybridization analyses...
  44. Spectral imaging for automated malignant blast counting
    Richard Levenson; Fiscal Year: 2007
    ..Double-immunophenotyping permits largely unambiguous detection, but such labeling strategies are not supported by standard (non-..
  45. PEDIATRIC ONCOLOGY GROUP
    Faith Kung; Fiscal Year: 2002
    ..POG led in the investigation in the immunophenotyping of acute lymphoblastic leukemia, NTX polyglutamates accumulation in leukemic cells, and N-myc gene ..
  46. BD Biosciences LSR Flow Cytometer
    Richard Hardy; Fiscal Year: 2002
    ..The other instrument, a BD FACScan, is widely used for simple immunophenotyping (2 color, P1) and for cell cycle analysis, but cannot perform more complex multi-color analyses that are ..
  47. PERSISTENT FETAL CELLS IN THE IMMUNOPATHOGENESIS OF SSC
    Carol Artlett; Fiscal Year: 2002
    ..of affected lung, skin, and kidneys in patient with early disease by a combination of magnetic cell sorting, immunophenotyping, and FISH...
  48. Novel Method for Sample Processing
    Yajuan Jiang; Fiscal Year: 2006
    ..biological specimen processing conditions such as fixation and permeabilization, used for intracellular immunophenotyping, is a well documented phenomenon...
  49. ORGAN CULTURE TO STUDY HIV TRANSMISSION
    Phalguni Gupta; Fiscal Year: 2003
    ..of HIV- I across the tissue will be monitored by the infectivity assay in cMAGI cells, and simultaneous immunophenotyping and in situ hybridization for HIV- I RNA Information from this project will be important for our ..
  50. Method for Analyzing and Isolating Stem Cells
    Yajuan Jiang; Fiscal Year: 2007
    ..Combining immunophenotyping with secretory profile is powerful approach for both research and eventual therapeutic applications...
  51. MONOCLONAL ANTIBODIES/SMALL CELL CARCINOMA OF THE LUNG
    Edward Ball; Fiscal Year: 1993
    ..In particular, we will undertake a prospective evaluation of SCCL tumor immunophenotyping for the purpose of establishing meaningful clinical correlations...
  52. FUNCTIONAL CHARTACTERIZATION OF FINE T CELL SUBSETS
    Leonard Herzenberg; Fiscal Year: 1999
    ..of T cells and allow the unraveling of the considerable confusion and controversy surrounding the AIDS immunophenotyping literature...
  53. RETROVIRAL VECTOR-MEDIATED TRANSFER OF NEF GENE
    Donald Kohn; Fiscal Year: 1991
    ..on normal cellular functions including growth properties and differentiation, surface antigen expression by immunophenotyping, and cytokine production...
  54. Determinants of asthma severity in Harlem (DASH)
    Jean Ford; Fiscal Year: 2005
    ..Our approach is to apply modern technologies (haplotype analysis; immunophenotyping) toward development of a mechanism-based framework for understanding pathways that underlie asthma severity ..
  55. The Effect Alcohol on SIV Pathogenesis
    Ronald Veazey; Fiscal Year: 2007
    ..Changes in viral target cells, naive and memory T cell immunophenotyping, chemokine receptor expression, cytokine and SIV-specific cytotoxic T cell (CTL) production, and levels of ..
  56. Research 2 Laser Analytical Flow Cytometer
    Bruce Pitt; Fiscal Year: 2004
    ..Donnenberg) and Center for Biological Imaging (Dr. Watkins). Applications will include multicolor immunophenotyping, physiological studies on transfected cells, signaling and apoptosis, stem cell biology and microbiology...
  57. Beta CELL PROGENITORS AND BONE MARROW MICROENVIRONMENT
    Dario Campana; Fiscal Year: 2004
    ..The proposed studies will further characterize the antileukemic activity of BAY 36-1677 and identify synergistically interacting compounds. ..
  58. IMMUNOGLOBULIN GENE ARRANGEMENT/EXPRESSION IN LEUKEMIA
    Dario Campana; Fiscal Year: 2005
    ..The development of new automated MRD assays and the identification of new markers of leukemia should improve residual disease evaluation and thereby improve risk-directed therapy selection for every child with ALL. ..
  59. DETECTION AND THERAPY OF RESIDUAL LEUKEMIA IN CHILDREN
    Dario Campana; Fiscal Year: 2006
    ..Success in this endeavor could radically improve remission studies in these patients by overcoming the practical and ethical constraints posed by sequential bone marrow aspirations ..
  60. Cell Therapy of Refractory Leukemia
    Dario Campana; Fiscal Year: 2010
    ..The research proposed should not only spur clinical studies of immune cells in patients with refractory ALL but also facilitate the development of effective cell therapies for other neoplasms. ..
  61. Clinical Significance of Residual Myeloid Leukemia
    Dario Campana; Fiscal Year: 2010
    ..Studies in this aim will determine whether higher levels of blood MRD and of circulating leukemic stem cells correlate with a higher risk of relapse. ..