immunologic cytotoxicity tests

Summary

Summary: The demonstration of the cytotoxic effect on a target cell of a lymphocyte, a mediator released by a sensitized lymphocyte, an antibody, or complement.

Top Publications

  1. ncbi A role for IFN-gamma in primary and secondary immunity generated by NK cell-sensitive tumor-expressing CD80 in vivo
    Janice M Kelly
    Cancer Immunology Program, Sir Donald and Lady Trescowthick Laboratories, Peter MacCallum Cancer Institute, East Melbourne, Victoria, Australia
    J Immunol 168:4472-9. 2002
  2. pmc Distinct role of lymphocyte function-associated antigen-1 in mediating effective cytolytic activity by cytotoxic T lymphocytes
    Nadia Anikeeva
    Department of Microbiology and Immunology and Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 102:6437-42. 2005
  3. ncbi A direct role for NKG2D/MICA interaction in villous atrophy during celiac disease
    Sophie Hue
    Equipe Avenir Inserm, Hopital Necker Enfants Malades, 75015 Paris, France
    Immunity 21:367-77. 2004
  4. ncbi Final steps of natural killer cell maturation: a model for type 1-type 2 differentiation?
    M J Loza
    Kimmel Cancer Center, Jefferson Medical College, Department of Microbiology and Immunology, Philadelphia, PA, USA
    Nat Immunol 2:917-24. 2001
  5. ncbi Innate direct anticancer effector function of human immature dendritic cells. I. Involvement of an apoptosis-inducing pathway
    Bratislav M Janjic
    University of Pittsburgh Cancer Institute, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15231, USA
    J Immunol 168:1823-30. 2002
  6. ncbi Immature CD4- CD103+ rat dendritic cells induce rapid caspase-independent apoptosis-like cell death in various tumor and nontumor cells and phagocytose their victims
    Benjamin Trinité
    Institut National de la Santé et de la Recherche Médicale Unité 643, Institut de Transplantation et de Recherche en Transplantation, Nantes University Hospital, Nantes, France
    J Immunol 175:2408-17. 2005
  7. ncbi LFA-1 contributes an early signal for NK cell cytotoxicity
    Domingo F Barber
    Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    J Immunol 173:3653-9. 2004
  8. ncbi Perforin and interferon-gamma activities independently control tumor initiation, growth, and metastasis
    S E Street
    Cancer Immunology, Peter MacCallum Cancer Institute, Victoria, Australia
    Blood 97:192-7. 2001
  9. ncbi NK cell activation by dendritic cells is dependent on LFA-1-mediated induction of calcium-calmodulin kinase II: inhibition by HIV-1 Tat C-terminal domain
    Alessandro Poggi
    Laboratory of Immunology, National Institute for Cancer Research, Genoa, Italy
    J Immunol 168:95-101. 2002
  10. ncbi Cutting edge: rapid in vivo killing by memory CD8 T cells
    Daniel L Barber
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USA
    J Immunol 171:27-31. 2003

Research Grants

  1. Vulnerability to Smallpox Due to Declining CTL Immunity
    Innocent Mbawuike; Fiscal Year: 2003
  2. TH1 CYTOKINES AND IMPAIRED CD8* CTLS IN ELDERLY
    Innocent Mbawuike; Fiscal Year: 2001
  3. Regulation of Polyoma Virus-Specific CD8+ T Cells
    ARON LUKACHER; Fiscal Year: 2007
  4. COCAINE SMOKING EFFECTS ON LUNG IMMUNITY & HOST DEFENSE
    Michael Roth; Fiscal Year: 2006
  5. Exploiting porcine cytomegalovirus stealth mechanisms
    MARK CREW; Fiscal Year: 2005
  6. HSV Immunotherapeutic Vaccine
    David Koelle; Fiscal Year: 2005
  7. Intradermal vaccinia:T-cell specificity and skin homing
    David Koelle; Fiscal Year: 2005
  8. Ligand for Natural Cytotoxicity Receptor NKp44
    PORUNELLOOR MATHEW; Fiscal Year: 2008
  9. Cellular Immunity to BK Virus
    Simon Lacey; Fiscal Year: 2005
  10. HIV-1 Suppression by MHC class I restricted CD8 T Cells
    Paul Goepfert; Fiscal Year: 2008

Detail Information

Publications213 found, 100 shown here

  1. ncbi A role for IFN-gamma in primary and secondary immunity generated by NK cell-sensitive tumor-expressing CD80 in vivo
    Janice M Kelly
    Cancer Immunology Program, Sir Donald and Lady Trescowthick Laboratories, Peter MacCallum Cancer Institute, East Melbourne, Victoria, Australia
    J Immunol 168:4472-9. 2002
    ..Our findings demonstrate a key role for IFN-gamma in innate and adaptive immunity triggered by CD80 expression on tumor cells...
  2. pmc Distinct role of lymphocyte function-associated antigen-1 in mediating effective cytolytic activity by cytotoxic T lymphocytes
    Nadia Anikeeva
    Department of Microbiology and Immunology and Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 102:6437-42. 2005
    ..These data provide evidence that LFA-1 delivers a distinct signal essential for directing released cytolytic granules to the surface of antigen-bearing target cells to mediate the effective destruction of these cells by CTL...
  3. ncbi A direct role for NKG2D/MICA interaction in villous atrophy during celiac disease
    Sophie Hue
    Equipe Avenir Inserm, Hopital Necker Enfants Malades, 75015 Paris, France
    Immunity 21:367-77. 2004
    ..This supports a key role for MIC/NKG2D in the activation of intraepithelial immunity in response to danger...
  4. ncbi Final steps of natural killer cell maturation: a model for type 1-type 2 differentiation?
    M J Loza
    Kimmel Cancer Center, Jefferson Medical College, Department of Microbiology and Immunology, Philadelphia, PA, USA
    Nat Immunol 2:917-24. 2001
    ..We propose that the cytokine environment regulates a type 2-->0-->1 developmental progression, with IL-12 needed for terminal differentiation and IL-4 delaying this process, rather than a type 1 versus type 2 decision of a type 0 cell...
  5. ncbi Innate direct anticancer effector function of human immature dendritic cells. I. Involvement of an apoptosis-inducing pathway
    Bratislav M Janjic
    University of Pittsburgh Cancer Institute, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15231, USA
    J Immunol 168:1823-30. 2002
    ..Overall, our data demonstrate that human immature DCs are capable of inducing apoptosis in cancer cells and thus to both directly mediate anticancer activity and initiate processing of cellular tumor Ags...
  6. ncbi Immature CD4- CD103+ rat dendritic cells induce rapid caspase-independent apoptosis-like cell death in various tumor and nontumor cells and phagocytose their victims
    Benjamin Trinité
    Institut National de la Santé et de la Recherche Médicale Unité 643, Institut de Transplantation et de Recherche en Transplantation, Nantes University Hospital, Nantes, France
    J Immunol 175:2408-17. 2005
    ..Taken together, these data indicate that rat immature CD4- CD103+ DC mediate an unusual cytotoxic activity and can use this function to efficiently acquire Ag from live cells...
  7. ncbi LFA-1 contributes an early signal for NK cell cytotoxicity
    Domingo F Barber
    Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    J Immunol 173:3653-9. 2004
    ..Thus, in contrast to T cells, in which even adhesion through LFA-1 is dependent on signals from other receptors, NK cells receive early activation signals directly through LFA-1...
  8. ncbi Perforin and interferon-gamma activities independently control tumor initiation, growth, and metastasis
    S E Street
    Cancer Immunology, Peter MacCallum Cancer Institute, Victoria, Australia
    Blood 97:192-7. 2001
    ....
  9. ncbi NK cell activation by dendritic cells is dependent on LFA-1-mediated induction of calcium-calmodulin kinase II: inhibition by HIV-1 Tat C-terminal domain
    Alessandro Poggi
    Laboratory of Immunology, National Institute for Cancer Research, Genoa, Italy
    J Immunol 168:95-101. 2002
    ..These data provide evidence that exogenous Tat inhibits NK cell activation occurring upon contact with DC: this mechanism might contribute to the impairment of natural immunity in HIV-1 infection...
  10. ncbi Cutting edge: rapid in vivo killing by memory CD8 T cells
    Daniel L Barber
    Emory Vaccine Center and Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USA
    J Immunol 171:27-31. 2003
    ..These data indicate that virus specific memory CD8 T cells can rapidly acquire cytotoxic function upon re-exposure to Ag and are much more efficient killers in vivo than previously appreciated...
  11. pmc Vav1 dephosphorylation by the tyrosine phosphatase SHP-1 as a mechanism for inhibition of cellular cytotoxicity
    Christopher C Stebbins
    Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
    Mol Cell Biol 23:6291-9. 2003
    ..Such a mechanism explains how inhibitory receptors can block activating signals induced by different receptors...
  12. ncbi Cutting edge: rapid in vivo CTL activity by polyoma virus-specific effector and memory CD8+ T cells
    Anthony M Byers
    Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Immunol 171:17-21. 2003
    ..These findings strongly support the concept that a cytotoxic effector-memory CD8(+) T cell population operates in vivo to control this persistent viral infection...
  13. pmc Multiple HLA class II-restricted melanocyte differentiation antigens are recognized by tumor-infiltrating lymphocytes from a patient with melanoma
    Paul F Robbins
    Surgery Branch, National Cancer Institute, National Institutes of Health, Building 10, Room 2B42, Bethesda, MD 20892 7502, USA
    J Immunol 169:6036-47. 2002
    ..These observations provide an impetus to develop strategies directed toward generating HLA class II-restricted tumor-reactive T cells...
  14. pmc Recognition of vaccinia virus-infected cells by human natural killer cells depends on natural cytotoxicity receptors
    Susan E Chisholm
    Division of Immunology, Department of Pathology, University of Cambridge, UK
    J Virol 80:2225-33. 2006
    ..The more general implications of these data for the processes of innate immune recognition are discussed...
  15. ncbi The fluorolysis assay, a highly sensitive method for measuring the cytolytic activity of T cells at very low numbers
    Norbert Kienzle
    The Queensland Institute of Medical Research and the Cooperative Research Centre for Vaccine Technology, University of Queensland, Brisbane QLD 4029, Australia
    J Immunol Methods 267:99-108. 2002
    ....
  16. pmc Beyond 51Cr release: New methods for assessing HIV-1-specific CD8+ T cell responses in peripheral blood and mucosal tissues
    B L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, 94103, USA
    Clin Exp Immunol 130:172-82. 2002
    ..This review focuses on recent developments in the assessment of HIV-1-specific T cell responses in peripheral blood and tissues, with a particular emphasis on flow cytometry-based approaches...
  17. ncbi A Structural basis for the association of DAP12 with mouse, but not human, NKG2D
    David B Rosen
    Department of Microbiology and Immunology and the Cancer Research Institute, University of California, San Francisco, CA 94143, USA
    J Immunol 173:2470-8. 2004
    ..Further, we show that human NKG2D is incapable of associating with DAP12 and provide evidence that structural differences in the transmembrane of mouse and human NKG2D account for the species-specific difference for this immune receptor...
  18. ncbi SV40 Immortalization of feline fibroblasts as targets for MHC-restricted cytotoxic T-cell assays
    S Koksoy
    Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd, Columbus, OH 43210, USA
    Vet Immunol Immunopathol 79:285-95. 2001
    ....
  19. ncbi Fluorescent antigen-transfected target cell cytotoxic T lymphocyte assay for ex vivo detection of antigen-specific cell-mediated cytotoxicity
    Carel A van Baalen
    Department of Virology, Erasmus MC, University Medical Center and Postgraduate School of Molecular Medicine, Rotterdam, The Netherlands
    J Infect Dis 192:1183-90. 2005
    ..The FATT-CTL assay provides a versatile tool that will advance our understanding of cell-mediated immunity...
  20. ncbi FL-CTL assay: fluorolysometric determination of cell-mediated cytotoxicity using green fluorescent protein and red fluorescent protein expressing target cells
    Kong Chen
    Louisiana State University Health Sciences Center, Gene Therapy Program, Department of Genetics and Medicine, 533 Bolivar Street, New Orleans, LA 70112, USA
    J Immunol Methods 300:100-14. 2005
    ..Thus, this FL-CTL assay is highly sensitive, reliable, reproducible, economical, convenience and supports broad applications compared to conventional CTL assays...
  21. ncbi Expression of the interleukin-21 gene in murine colon carcinoma cells generates systemic immunity in the inoculated hosts
    Shin ichi Ugai
    Department of Pathology, Chiba Cancer Centre Research Institute, Japan
    Cancer Gene Ther 10:187-92. 2003
    ..Spleen cells from Colon 26/IL-21-tumor- but not parent-tumor-bearing mice had lytic activity to YAC-1 cells. These data suggest that expression of IL-21 in tumors induces T- and NK-cell-dependent antitumor effects...
  22. pmc Induction of natural killer cell responses by ectromelia virus controls infection
    April Keim Parker
    Department of Molecular Microbiology and Immunology, Saint Louis University Health Sciences Center, 1402 South Grand Blvd, St Louis, MO 63104, USA
    J Virol 81:4070-9. 2007
    ..These results confirm a crucial role for NK cells in controlling an ECTV infection...
  23. ncbi Cutting edge: predetermined avidity of human CD8 T cells expanded on calibrated MHC/anti-CD28-coated microspheres
    Steffen Walter
    Department of Immunology, Institute for Cell Biology, Division of Hematology and Oncology, University of Tubingen, Tubingen, Germany
    J Immunol 171:4974-8. 2003
    ..We believe that the possibility to raise Ag-specific T cells with predetermined avidity will be crucial for the future use of aAPCs in immunotherapeutical settings...
  24. ncbi A ligand for the murine NK activation receptor Ly-49D: activation of tolerized NK cells from beta 2-microglobulin-deficient mice
    Hiroshi Furukawa
    Howard Hughes Medical Institute, Rheumatology Division, Barnes Jewish Hospital and Washington University School of Medicine, St Louis, MO 63110 1093, USA
    J Immunol 169:126-36. 2002
    ....
  25. ncbi Induction of polyclonal prostate cancer-specific CTL using dendritic cells transfected with amplified tumor RNA
    A Heiser
    Department of Surgery, Division of Urology, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 166:2953-60. 2001
    ....
  26. ncbi Innate direct anticancer effector function of human immature dendritic cells. II. Role of TNF, lymphotoxin-alpha(1)beta(2), Fas ligand, and TNF-related apoptosis-inducing ligand
    Ganwei Lu
    University of Pittsburgh Cancer Institute, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15231, USA
    J Immunol 168:1831-9. 2002
    ..Overall, our data demonstrate that DCs are fully equipped for an efficient direct apoptotic killing of cancer cells and suggest that this mechanism may play a critical role in both afferent and efferent anti-tumor immunity...
  27. pmc CD40-activated human B cells: an alternative source of highly efficient antigen presenting cells to generate autologous antigen-specific T cells for adoptive immunotherapy
    J L Schultze
    Department of Adult Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Invest 100:2757-65. 1997
    ..We, therefore, contend that CD40-activated B cells are an alternative source of highly efficient APCs with which to generate antigen-specific T cells ex vivo for autologous adoptive immunotherapy...
  28. ncbi Interleukin-21 activates cytotoxic T lymphocytes and natural killer cells to generate antitumor response in mouse renal cell carcinoma
    Masafumi Kumano
    Division of Urology, Kobe University Graduate School of Medicine, Kobe, Japan
    J Urol 178:1504-9. 2007
    ..We evaluated the antitumor effects of IL-21 gene transfer into mouse RenCa renal cell carcinoma cells, so that cells could spontaneously secrete IL-21. We also investigated the mechanisms underlying this antitumor effect...
  29. ncbi A sensitive flow cytometry-based cytotoxic T-lymphocyte assay through detection of cleaved caspase 3 in target cells
    Liwei He
    Immunology Platform, Aventis Pasteur Research and Development, Connaught Campus Toronto, Ontario, Canada M2R 3T4
    J Immunol Methods 304:43-59. 2005
    ....
  30. ncbi The gp49B1 inhibitory receptor regulates the IFN-gamma responses of T cells and NK cells
    Xiaogang Gu
    Center for Blood Research and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 170:4095-101. 2003
    ..We conclude that gp49B1 is a unique inhibitory receptor that is induced in multiple lineages of innate and adaptive immune cells during an infection and controls their IFN-gamma, but not cytotoxic responses...
  31. ncbi Tolerance induction by veto CTLs in the TCR transgenic 2C mouse model. I. Relative reactivity of different veto cells
    Shlomit Reich-Zeliger
    Department of Immunology, Weizmann Institute of Science, Rehovot, Israel
    J Immunol 173:6654-9. 2004
    ..The latter cells as well as nonactivated CD4(+) or NK cells were shown to be completely devoid of veto activity...
  32. ncbi Selective induction of high avidity CTL by altering the balance of signals from APC
    SangKon Oh
    Molecular Immunogenetics and Vaccine Research Section, Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 170:2523-30. 2003
    ..Our data also suggested that dendritic cells play an important role in induction of high avidity CD8(+) CTL in vivo. This strategy to selectively induce higher avidity CTL may lead to more effective vaccines for viruses and cancer...
  33. ncbi Enhanced induction of anti-tumor immunity in human and mouse by dendritic cells pulsed with recombinant TAT fused human survivin protein
    Hyun Il Cho
    Department of Microbiology, College of Medicine, Catholic University of Korea, 505 Banpo Dong, Seocho Gu, Seoul 137 701, South Korea
    Cancer Lett 258:189-98. 2007
    ..These results suggest that vaccination with DCs pulsed with tatSVV is an effective way for the SVV-targeting cancer vaccine to induce an effective anti-tumor responses...
  34. ncbi The role of T cell antagonism and original antigenic sin in genetic immunization
    Rana A K Singh
    Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA
    J Immunol 169:6779-86. 2002
    ....
  35. ncbi Syngeneic mixed lymphocyte reaction (SMLR) with dendritic cells: direct visualization of dividing T cell subsets in SMLR
    Toshimasa Aranami
    Division of Immunobiology, Research Section of Pathophysiology, Institute for Genetic Medicine, Hokkaido University, Kita 15 Nishi 7 Kita ku, Sapporo 060 0815, Japan
    Cell Immunol 217:67-77. 2002
    ....
  36. ncbi Identification of effector-memory CMV-specific T lymphocytes that kill CMV-infected target cells in an HLA-E-restricted fashion
    Paola Mazzarino
    Dipartimento di Oncologia, Biologia e Genetica, Universita degli Studi di Genova, Genova, Italy
    Eur J Immunol 35:3240-7. 2005
    ..Our data suggest that HLA-E-restricted CTL may represent an additional effector cell type involved in defenses against HCMV, a virus which escapes the control exerted by conventional CTL or NK cells...
  37. ncbi The adjuvant effects of Mycobacterium tuberculosis heat shock protein 70 result from the rapid and prolonged activation of antigen-specific CD8+ T cells in vivo
    Lisa A E Harmala
    Department of Laboratory Medicine and Pathology, Center for Immunology, Cancer Center, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    J Immunol 169:5622-9. 2002
    ..These studies demonstrate that the potent adjuvant effect of M. tuberculosis hsp70 results from the relatively complete, rapid, and durable activation of Ag-specific CD8(+) T cells...
  38. ncbi High-affinity HLA-A(*)02.01 peptides from parathyroid hormone-related protein generate in vitro and in vivo antitumor CTL response without autoimmune side effects
    Guido Francini
    Division of Medical Oncology, Institut National de la Santé et de la Recherche Médicale Unité d Immunité Cellulaire Antivirale, Institut Pasteur, Paris, France
    J Immunol 169:4840-9. 2002
    ..In this study we describe two immunogenic and toxic-free PTH-rP peptides as valid candidates for the design of peptide-based vaccination strategies against prostate cancer and bone metastases from the most common epithelial malignancies...
  39. ncbi Most murine CD8+ intestinal intraepithelial lymphocytes are partially but not fully activated T cells
    Heuy Ching Wang
    Dental Branch, Department of Basic Sciences, University of Texas Health Science Center, Houston 77030, USA
    J Immunol 169:4717-22. 2002
    ..These data also imply that under appropriate conditions it is possible for T cells to be sustained in a state of partial activation...
  40. ncbi Targeting positive regulatory domain I-binding factor 1 and X box-binding protein 1 transcription factors by multiple myeloma-reactive CTL
    Carina Lotz
    Department of Hematology and Oncology, Johannes Gutenberg University, Mainz, Germany
    J Immunol 175:1301-9. 2005
    ..We conclude that transcription factors governing terminal cellular differentiation may provide MM- and tumor-associated CTL epitopes...
  41. ncbi Thy-1 signaling in the context of costimulation provided by dendritic cells provides signal 1 for T cell proliferation and cytotoxic effector molecule expression, but fails to trigger delivery of the lethal hit
    S M Mansour Haeryfar
    Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada
    J Immunol 171:69-77. 2003
    ....
  42. ncbi Langerhans cells derived from genetically modified human CD34+ hemopoietic progenitors are more potent than peptide-pulsed Langerhans cells for inducing antigen-specific CD8+ cytolytic T lymphocyte responses
    Jianda Yuan
    Laboratory of Cellular Immunobiology, Division of Hematologic Oncology, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Immunol 174:758-66. 2005
    ..LCs genetically modified to express fluMP are also more potent stimulators of Ag-specific CD8(+) T cell responses than are peptide-pulsed LCs...
  43. ncbi Enhanced antilymphoma efficacy of CD19-redirected influenza MP1-specific CTLs by cotransfer of T cells modified to present influenza MP1
    Laurence J N Cooper
    Beckman Research Institute, Division of Pediatric Hematology Oncology, City of Hope National Medical Center, Duarte, CA 91010 3000, USA
    Blood 105:1622-31. 2005
    ..Engineered T-APCs are a novel and versatile antigen-delivery system for generating antigen-specific T cells in vitro and enhancing the in vivo effector functioning of CAR-redirected antitumor effector cells...
  44. ncbi Antigen-specific T cells maintain an effector memory phenotype during persistent Trypanosoma cruzi infection
    Diana L Martin
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA
    J Immunol 174:1594-601. 2005
    ..These results suggest that T cells maintained in mice with chronic T. cruzi infection are fully functional memory cells that cannot be easily categorized in the current central/effector memory paradigm...
  45. ncbi Rapid conversion of effector mechanisms from NK to T cells during virus-induced lysis of allogeneic implants in vivo
    Michael A Brehm
    Department of Pathology, University of Massachusetts Medical School, Worcester, 01655, USA
    J Immunol 174:6663-71. 2005
    ..This rapid generation of allospecific CTL activity during a viral infection preceded the peak of viral epitope-specific T cell responses, as detected by in vivo or in vitro cytotoxicity assays...
  46. ncbi Generation of potent antitumor CTL from patients with multiple myeloma directed against HM1.24
    Steven B Rew
    Department of Haematology, Royal Free and University College Medical School, London, United Kingdom
    Clin Cancer Res 11:3377-84. 2005
    ..The purpose of this work was to test the suitability of the HM1.24 antigen as a CTL target for immunotherapy of patients with multiple myeloma...
  47. ncbi Age-dependent hantavirus-specific CD8(+) T-cell responses in mice infected with Hantaan virus
    K Araki
    Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
    Arch Virol 149:1373-82. 2004
    ....
  48. ncbi Vaccination with dendritic cells pulsed in vitro with tumor antigen conjugated to cholera toxin efficiently induces specific tumoricidal CD8+ cytotoxic lymphocytes dependent on cyclic AMP activation of dendritic cells
    J B Sun
    Department of Medical Microbiology and Immunology, and Göteborg University Vaccine Research Institute GUVAX, Goteborg University, S 405 30, Goteborg, Sweden
    Clin Immunol 112:35-44. 2004
    ....
  49. ncbi HLA-E-restricted recognition of human cytomegalovirus by a subset of cytolytic T lymphocytes
    Chiara Romagnani
    Dipartimento di Medicinia Sperimentale, Centro di Eccellenza per Ricerche Biomediche, Universita degli Studi di Genova, Genova, Italy
    Hum Immunol 65:437-45. 2004
    ..This broad ability to recognize and kill allogeneic cells may pose serious problems in transplantation...
  50. ncbi Definition of an immunogenic region within the ovarian tumor antigen stratum corneum chymotryptic enzyme
    Kristina L Bondurant
    Department of Microbiology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Clin Cancer Res 11:3446-54. 2005
    ..In this study, we tested the hypothesis that dendritic cells loaded with SCCE peptides will induce ovarian tumor antigen-specific CD8+ CTL responses and antigen-specific CD4+ helper T cell responses...
  51. ncbi Injection of IL-12 gene-transduced dendritic cells into mouse liver tumor lesions activates both innate and acquired immunity
    T Tatsumi
    Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, Suita, Japan
    Gene Ther 14:863-71. 2007
    ..These results revealed that IL-12 gene transfer is capable of improving the impaired functions of DC isolated from tumor-bearing hosts, and support the preclinical therapeutic efficacy of intrahepatic injection of AdIL12DC...
  52. ncbi Enhanced tumor responses to dendritic cells in the absence of CD8-positive cells
    Antoni Ribas
    Department of Medicine, Division of Hematology Oncology, University of California, Los Angeles, CA 90095, USA
    J Immunol 172:4762-9. 2004
    ..Elucidation of the signaling events that activate these effective cytotoxic cells and the putative suppressive mechanisms in a wild-type environment may provide means to enhance the clinical activity of DC-based approaches...
  53. ncbi The VITAL assay: a versatile fluorometric technique for assessing CTL- and NKT-mediated cytotoxicity against multiple targets in vitro and in vivo
    Ian F Hermans
    Tumour Immunology Unit, Nuffield Department of Medicine, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, UK
    J Immunol Methods 285:25-40. 2004
    ..The VITAL assay is, therefore, a sensitive technique allowing analysis of complex multi-epitope responses...
  54. ncbi Differential susceptibility of cells expressing allogeneic MHC or viral antigen to killing by antigen-specific CTL
    Koutetsu Lee
    Department of Physiology, Osaka Medical College, Takatsuki, Osaka, Japan
    Microbiol Immunol 48:15-25. 2004
    ..Taken together, these results suggest that not all in vivo tissues may be sensitive to CTL lysis...
  55. ncbi Mechanisms of superior anti-tumor cytotoxic response of interleukin 15-induced lymphokine-activated killer cells
    Oner Ozdemir
    Division of Hematology Oncology, Children s Hospital of Michigan, Wayne State University, 3901 Beaubien Blvd, Detroit, MI 48201 2196, USA
    J Immunother 28:44-52. 2005
    ..These results are encouraging for potential use of IL-15 as part of immunotherapy...
  56. ncbi The immunodominant, Ld-restricted T cell response to hepatitis B surface antigen (HBsAg) efficiently suppresses T cell priming to multiple Dd-, Kd-, and Kb-restricted HBsAg epitopes
    Reinhold Schirmbeck
    Institute of Medical Microbiology and Immunology, University of Ulm, Ulm, Germany
    J Immunol 168:6253-62. 2002
    ....
  57. ncbi Experimental study of the efficacy of combined use of cancer vaccine and interferon
    G P Potebnya
    R E Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
    Exp Oncol 29:102-5. 2007
    ..To study in in vivo model the efficacy of combined scheme of administration of cancer vaccine (CV) and interferon (IFN)...
  58. ncbi In vivo vaccination with tumor cell lysate plus CpG oligodeoxynucleotides eradicates murine glioblastoma
    Anhua Wu
    Department of Neurosurgery, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Immunother 30:789-97. 2007
    ..05). These results suggest direct vaccination with CpG/lysate provides an alternative and effective approach to induce host antitumor immunity and warrants clinical investigation in the immunotherapy of cancer...
  59. ncbi Cell death mediated by alloreactive cytotoxic T cells via the granule exocytosis or the Fas pathway is independent of p34cdc2 kinase: Fas dependent killing of cells arrested in the cell cycle
    P Waring
    Division of Immunology and Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia
    Immunol Cell Biol 79:264-73. 2001
    ..This lysis was accompanied by an increase in cell surface Fas expression. Olomoucine induced cell cycle arrest and expression of Fas was reversible and when cells re-entered the cell cycle, surface expression of Fas was lost...
  60. ncbi Generation of EBV-specific T cells for adoptive immunotherapy: a novel protocol using formalin-fixed stimulator cells to increase biosafety
    Markus H Hammer
    Interdisciplinary Transplant Laboratory Nephrology Medical Immunology, Charite Universitatsmedizin Berlin, Germany
    J Immunother 30:817-24. 2007
    ..Furthermore, fixation will allow the use of transgenic LCLs (eg, with cytomegalovirus or tumor antigens) and thereby facilitate the generation of antigen-specific T cells directed against pathogens other than EBV...
  61. ncbi Genetically modified HLA class I molecules able to inhibit human NK cells without provoking alloreactive CD8+ CTLs
    Alexandra Sharland
    Laboratory of Molecular and Cellular Immunology, Transplantation Biology Research Center, Boston, MA 02129, USA
    J Immunol 168:3266-74. 2002
    ..Such genetically modified molecules may have potential therapeutic applications in the prevention of delayed xenograft rejection and in the facilitation of allogeneic and xenogeneic bone marrow engraftment...
  62. ncbi Critical role for activation of antigen-presenting cells in priming of cytotoxic T cell responses after vaccination with virus-like particles
    Tazio Storni
    Cytos Biotechnology, AG, Department of Dermatology and Institute of Experimental Immunology, University Hospital, and Elektronenmikroskopisches Zentrallabor Universität, Zurich, Switzerland
    J Immunol 168:2880-6. 2002
    ..Thus, while VLPs alone are inefficient at inducing CTL responses, they become very powerful vaccines if applied together with substances that activate APCs...
  63. ncbi MHC class I-restricted determinants on the glutamic acid decarboxylase 65 molecule induce spontaneous CTL activity
    A Quinn
    Division of Immune Regulation, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    J Immunol 167:1748-57. 2001
    ....
  64. pmc Antigen delivered by anthrax lethal toxin induces the development of memory CD8+ T cells that can be rapidly boosted and display effector functions
    Christine A Shaw
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 76:1214-22. 2008
    ..Finally, we demonstrated that mice that received prime-boost immunizations of LT-antigen proteins were more protected in a Listeria monocytogenes challenge model than mice that received only one immunization...
  65. ncbi An improved protocol for measuring cytotoxic T cell activity in anatomic compartments with low cell numbers
    T Ostler
    Children's Hospital, University of Freiburg, Mathildenstrasse 1, D-79106, Freiburg, Germany
    J Immunol Methods 257:155-61. 2001
    ..Flow cytometric analysis of interferon-gamma production by virus-specific T cells including appropriate controls was achieved with as few as 10(5) effector cells...
  66. ncbi Activation of NK cell-mediated cytotoxicity by a SAP-independent receptor of the CD2 family
    A Bouchon
    Basel Institute for Immunology, Basel, Switzerland
    J Immunol 167:5517-21. 2001
    ..Thus, CRACC is a unique CD2-like receptor which mediates NK cell activation through a SAP-independent extracellular signal-regulated kinase-mediated pathway...
  67. ncbi Vaccination with allogeneic GM-CSF gene-modified lung cancer cells: antitumor activity comparing with that induced by autologous vaccine
    Hui Li
    Department of Immunology, Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tianjin, China
    Cancer Biother Radiopharm 22:790-8. 2007
    ..The immunogenic and antitumor activity of allogeneic vaccine was compared with that of autologous cancer cell vaccine...
  68. ncbi B-Myb overexpression results in activation and increased Fas/Fas ligand-mediated cytotoxicity of T and NK cells
    M A Powzaniuk
    Department of Microbiology and Immunology, Kimmel Cancer Center, Jefferson Medical College, Philadelphia, PA 19107, USA
    J Immunol 167:242-9. 2001
    ..Therefore, in addition to its well-established role in proliferation and differentiation, B-myb also appears to be involved in activation of NK and T cells and in their regulation of Fas/Fas ligand-mediated cytotoxicity..
  69. ncbi Activation-induced nonresponsiveness: a Th-dependent regulatory checkpoint in the CTL response
    Ee Loon Tham
    Center for Immunology, Department of Biochemistry, University of Minnesota, Minneapolis, MN 55455, USA
    J Immunol 168:1190-7. 2002
    ..Once permission is given, in the form of IL-2, to pass this checkpoint, the CTL can make a prolonged response to persisting Ag in the absence of further CD4 T cell help...
  70. ncbi Generation of EBV-specific CD4+ cytotoxic T cells from virus naive individuals
    Barbara Savoldo
    Center for Cell and Gene Therapy and Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    J Immunol 168:909-18. 2002
    ..In addition, our method for selecting EBV-specific CTL from naive individuals by precursor cell enrichment may be applicable to the immunotherapy of cancer patients with a low frequency of tumor- or virus-specific CTL...
  71. ncbi Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatment
    Carmen Scheibenbogen
    Medizinische Klinik III, Hematology, Oncology and Transfusion Medicine, Universitaetsklinikum Benjamin Franklin, Freie Universitat Berlin, Berlin, Germany
    Int J Cancer 98:409-14. 2002
    ....
  72. ncbi Investigation of antigen delivery route in vivo and imune-boosting effects mediated by pH-sensitive liposomes encapsulated with K(b)-restricted CTL epitope
    Ki Young Lee
    Department of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei University, 134 Shinchon dong, Seodaemun gu, Seoul, 120 749, South Korea
    Biochem Biophys Res Commun 292:682-8. 2002
    ..These results suggest that pH-sensitive liposome formula functions as a potential adjuvant of peptide antigens and is useful for the induction of antigen specific CTLsv in vivo...
  73. ncbi Multiple Chlamydia pneumoniae antigens prime CD8+ Tc1 responses that inhibit intracellular growth of this vacuolar pathogen
    Benjamin Wizel
    Center for Pulmonary and Infectious Disease Control, Department of Microbiology and Immunology, University of Texas Health Center, Tyler 75708, USA
    J Immunol 169:2524-35. 2002
    ....
  74. ncbi Induction of murine gamma delta T cells cytotoxic for xenogeneic rat cells
    C Okumura
    Department of Plastic and Reconstructive Surgery, Nagasaki University School of Medicine, Japan
    J Immunol 154:1114-23. 1995
    ..V gamma 1 was detected as a major V gamma gene expressed in this gamma delta population by reverse transcriptase-PCR. Cytotoxicity for xenogeneic cells may represent one of major function of gamma delta T cells...
  75. ncbi Involvement of MHC class I molecule and ICAM-1 in the enhancement of adhesion and cytotoxic susceptibility to immune effector cells of tumor cells transfected with the interleukin (IL)-2, IL-4 or IL-6 gene
    X Cao
    Department of Immunology, Second Military Medical University, Shanghai, PR China
    J Cancer Res Clin Oncol 123:602-8. 1997
    ....
  76. ncbi Antigen-dependent release of IFN-gamma by cytotoxic T cells up-regulates Fas on target cells and facilitates exocytosis-independent specific target cell lysis
    Arno Mullbacher
    Division of Immunology and Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, Australia
    J Immunol 169:145-50. 2002
    ..The results are discussed as to the possible relevance of the phenomenon in efficiency and regulation of the Tc cell response to infections by viruses...
  77. ncbi Regulation of the CTL response by macrophage migration inhibitory factor
    R Abe
    Laboratories of. Vascular Biology and Medical Biochemistry, The Picower Institute for Medical Research, Manhasset, NY 11030, USA
    J Immunol 166:747-53. 2001
    ..These data provide the first evidence of an important role for MIF in the regulation and trafficking of anti-tumor T lymphocytes in vivo...
  78. ncbi A redundant role of the CD3 gamma-immunoreceptor tyrosine-based activation motif in mature T cell function
    M C Haks
    Division of Immunology, Division of Cellular Biochemistry, and Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    J Immunol 166:2576-88. 2001
    ..Therefore, if ITAMs can function in signal specification, it is likely that either the CD3delta and/or the CD3epsilon chains endow the TCR with qualitatively unique signaling functions...
  79. ncbi Branched and linear lipopeptide vaccines have different effects on primary CD4+ and CD8+ T-cell activation but induce similar tumor-protective memory CD8+ T-cell responses
    Adriana Baz
    Cooperative Research Centre for Vaccine Technology, Queensland Institute of Medical Research, Queensland 4029, Australia
    Vaccine 26:2570-9. 2008
    ..OVA epitope display was reduced in tumors that developed in some mice, suggesting CD8+ T-cell dependent selection...
  80. ncbi Innate immunity to viruses: control of vaccinia virus infection by gamma delta T cells
    L K Selin
    Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Immunol 166:6784-94. 2001
    ..Thus, gammadelta T cells can be mediators of innate immunity to viruses, having a significant impact on virus replication early in infection in the presence or absence of the adaptive immune response...
  81. ncbi Clinical significance of HLA class I alleles on postoperative prognosis of lung cancer patients in Japan
    Yoshika Nagata
    Second Department of Surgery, University of Occupational and Environmental Health, School of Medicine, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu City, Fukuoka 807 8555, Japan
    Lung Cancer 65:91-7. 2009
    ..We previously reported the correlation between HLA alleles and the postoperative prognosis of 204 patients with non-small cell lung cancer (NSCLC). The present study was based on 695 patients with NSCLC to confirm these correlations...
  82. pmc Evaluation of a novel mononuclear cell isolation procedure for serological HLA typing
    P Schlenke
    Institute of Immunology and Transfusion Medicine, University of Luebeck School of Medicine, D23538 Luebeck, Germany
    Clin Diagn Lab Immunol 5:808-13. 1998
    ..The decreasing viability after 48 h limits the use of both methods for HLA typing and HLA antibody screening...
  83. ncbi Unfavorable prognosis of patients with non-small cell lung carcinoma associated with HLA-A2
    T So
    Department of Surgery II, School of Medicine, University of Occupational and Environmental Health, Iseigaoka 1 1, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Lung Cancer 32:39-46. 2001
    ..We studied types of HLA class I antigens in 204 resected non-small cell lung carcinoma (NSCLC) patients and also examined its correlation with clinicopathologic features and prognosis...
  84. ncbi Association between pemphigus vulgaris and human leukocyte antigen in Han nation of northeast China
    Long Geng
    Department of Dermatology, First Affiliated Hospital, China Medical University, Shenyang 110001
    Chin Med Sci J 20:166-70. 2005
    ..To investigate the relationship between pemphigus vulgaris (PV) and human leukocyte antigen (HLA) in Han nation of northeast China...
  85. ncbi [Camouflage of HLA-I antigen in lymphocyte surface]
    Yin ze Zhang
    Department of Blood Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China
    Zhongguo Shi Yan Xue Ye Xue Za Zhi 11:622-4. 2003
    ..It is suggested that the specific reaction between HLA-I antigen of lymphocyte and HLA-I antibodies can be completely camouflaged by mPEG-BTC in PBS (pH 7.4) under 22 degrees C room temperature...
  86. ncbi Differential calcium response in HeLa and HeLa-Fas cells by cytotoxic T lymphocytes
    E Marion Schneider
    Sektion Experimentelle Anästhesiologie, Universitatsklinikum Ulm, Steinhovelstr 9, Ulm D 89075, Germany
    Biochem Biophys Res Commun 301:159-66. 2003
    ....
  87. ncbi Induction of cytotoxic granules in human memory CD8+ T cell subsets requires cell cycle progression
    Yuru Meng
    Department of Pathology, University of Chicago, Chicago, IL 60637, USA
    J Immunol 177:1981-7. 2006
    ..The fact that granule induction can be achieved through TCR and CD28 ligation has implications for restoring lytic effector function in the context of antitumor immunity...
  88. ncbi Upregulation of cystatin M during the progression of oropharyngeal squamous cell carcinoma from primary tumor to metastasis
    Nadarajah Vigneswaran
    Department of Diagnostic Sciences, UT Houston Dental Branch Stomatology, Room 3 094G, 6516 John Freeman Avenue, Houston, TX 77030 3402, USA
    Oral Oncol 39:559-68. 2003
    ..Thus, we propose that elevated cystatin M expression aids metastasis by blocking intrinsic cathepsin B activity and rescuing tumor cells from TNF-induced apoptosis...
  89. ncbi Inhibition by interleukin-18 of the growth of Dunn osteosarcoma cells
    Takuya Okamoto
    Departments of Pathology and Orthopedic Surgery, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo 663 8501, Japan
    J Interferon Cytokine Res 24:161-7. 2004
    ..These results indicate that IL-18 inhibits the growth of Dunn osteosarcoma cells in vivo by enhancing the cytotoxic activity of CD8(+) T lymphocytes through the FasL-Fas system...
  90. ncbi Testing for equine arteritis virus
    A A Cullinane
    Vet Rec 155:647-8. 2004
  91. ncbi Anti-SARS-CoV immunity induced by a novel CpG oligodeoxynucleotide
    Musheng Bao
    Department of Molecular Biology, College of Basic Medicine, Jilin University, Changchun 130021, China
    Clin Immunol 118:180-7. 2006
    ..Together, BW001 represents a novel type of CpG ODN and may have potential for the development of treatment and prevention for SARS as well as other viral associated diseases...
  92. pmc Effect of the V3 loop deletion of envelope glycoprotein on cellular responses and protection against challenge with recombinant vaccinia virus expressing gp160 of primary human immunodeficiency virus type 1 isolates
    Irena Kiszka
    Center for Neurovirology and Cancer Biology, Temple University, Philadelphia, Pennsylvania 19122, USA
    J Virol 76:4222-32. 2002
    ..The results from our studies provide insights into approaches for boosting the breadth of envelope-specific CTL responses...
  93. ncbi A functional role of HLA-G expression in human gliomas: an alternative strategy of immune escape
    Heinz Wiendl
    Department of Neurology, Medical School, University of Tubingen, Tubingen, Germany
    J Immunol 168:4772-80. 2002
    ..We conclude that the aberrant expression of HLA-G may contribute to immune escape in human glioblastoma...
  94. ncbi Degeneracy and repertoire of the human HIV-1 Gag p17(77-85) CTL response
    June Kan-Mitchell
    Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201, USA
    J Immunol 176:6690-701. 2006
    ..This study suggests that agonist sequences can replace aberrantly immunogenic native epitopes for the rational design of vaccines targeting HIV-1...
  95. ncbi Cord blood-derived plasma factor (CBPF) potentiates the low cytokinetic and immunokinetic profile of bone marrow cells in pesticide victims suffering from Acquired Aplastic Anaemia (AAA): an in vitro correlate
    Sujata Law
    Department of Haematology, Immunology and Immuno Haematology Laboratory, School of Tropical Medicine, C R Avenue, Kolkata, India
    Immunol Invest 35:209-25. 2006
    ....
  96. ncbi Functional modulation of expanded CD8+ synovial fluid T cells by NK cell receptor expression in HLA-B27-associated reactive arthritis
    Nicolas Dulphy
    Laboratoire d Immunologie et d Histocompatibilité, INSERM U396, Centre G Hayem, Universite Paris VII, Institut Universitaire d Hematologie, Hopital Saint Louis, Avenue C Vellefaux, 75475 Paris Cedex 10, France
    Int Immunol 14:471-9. 2002
    ....
  97. ncbi Dual functions of fractalkine/CX3C ligand 1 in trafficking of perforin+/granzyme B+ cytotoxic effector lymphocytes that are defined by CX3CR1 expression
    Miyuki Nishimura
    KAN Research Institute, Kyoto, Japan
    J Immunol 168:6173-80. 2002
    ....
  98. ncbi Vascular endothelial cell apoptosis induced by anti-donor non-MHC antibodies: a possible injury pathway contributing to chronic allograft rejection
    Gordon D Wu
    Department of Cardiothoracic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, 90033, USA
    J Heart Lung Transplant 21:1174-87. 2002
    ..Non-major histocompatibility complex (non-MHC) alloantibodies may play a pathogenic role in chronic rejection but remain poorly characterized...
  99. ncbi In vivo cross-presentation of a soluble protein antigen: kinetics, distribution, and generation of effector CTL recognizing dominant and subdominant epitopes
    D Nelson
    Department of Medicine, University of Western Australia, Nedlands, Australia
    J Immunol 165:6123-32. 2000
    ..Analysis of functional in vivo CTL responses demonstrated that, in the presence of strong ongoing responses to the dominant peptide, lytic activity of CTL directed at weaker epitopes is undetectable...
  100. ncbi Adenovirus DNA polymerase is recognized by human CD8+ T cells
    Amita Joshi
    Department of Microbiology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Gen Virol 90:84-94. 2009
    ..Pol epitopes were well conserved among different Ad serotypes. Therefore, Pol is a promising target for immunotherapy of Ad disease...
  101. ncbi Midkine promoter-driven suicide gene expression and -mediated adenovirus replication produced cytotoxic effects to immortalised and tumour cells
    L Yu
    Division of Pathology, Chiba Cancer Center Research Institute, 666 2 Nitona, Chuo Ku, Chiba 260 8717, Japan
    Eur J Cancer 40:1787-94. 2004
    ..The MK promoter-driven suicide gene therapy and -mediated replicative Ad can thereby produce cytotoxic effects to immortalized and tumour cells with minimal damage to normal cells...

Research Grants67

  1. Vulnerability to Smallpox Due to Declining CTL Immunity
    Innocent Mbawuike; Fiscal Year: 2003
    ..By correlating these responses with vaccinia lesion sizes in new vaccinees, markers of severe complications from vaccinia vaccination may be identified. ..
  2. TH1 CYTOKINES AND IMPAIRED CD8* CTLS IN ELDERLY
    Innocent Mbawuike; Fiscal Year: 2001
    ....
  3. Regulation of Polyoma Virus-Specific CD8+ T Cells
    ARON LUKACHER; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  4. COCAINE SMOKING EFFECTS ON LUNG IMMUNITY & HOST DEFENSE
    Michael Roth; Fiscal Year: 2006
    ..abstract_text> ..
  5. Exploiting porcine cytomegalovirus stealth mechanisms
    MARK CREW; Fiscal Year: 2005
    ..The Phase I project will be deemed successful when we have identified specific genes from PCMV which impart stealth to pig cells in vitro when exposed to cells from the human immune system. ..
  6. HSV Immunotherapeutic Vaccine
    David Koelle; Fiscal Year: 2005
    ....
  7. Intradermal vaccinia:T-cell specificity and skin homing
    David Koelle; Fiscal Year: 2005
    ..2) Measure E-selectin binding by vaccinia-specific CD8 cells, and 2.3) Compare CLA and E-selectin binding by vaccinia-specific T-cells in recent and distant vaccines. ..
  8. Ligand for Natural Cytotoxicity Receptor NKp44
    PORUNELLOOR MATHEW; Fiscal Year: 2008
    ..This information will be useful in developing immune based treatment for cancer and other diseases. [unreadable] [unreadable] [unreadable] [unreadable]..
  9. Cellular Immunity to BK Virus
    Simon Lacey; Fiscal Year: 2005
    ..Such information may be useful in guiding clinical management, and lay the foundation for possible future vaccines. ..
  10. HIV-1 Suppression by MHC class I restricted CD8 T Cells
    Paul Goepfert; Fiscal Year: 2008
    ..Our findings will have important implications fro HIV immunopathogenesis and vaccine design by supplying an HIV-1 specific CD8 T cell phenotype that results in efficient HIV-1 control. [unreadable] [unreadable] [unreadable]..
  11. Antiviral Therapy for Polyomavirus Infection
    ARON LUKACHER; Fiscal Year: 2008
    ..g., FDA-approved Gleevec) limit polyomavirus infection, a critical prelude for their potential therapeutic use to prevent polyomavirus reactivation in immunosuppressed individuals. [unreadable] [unreadable] [unreadable]..
  12. The role of dendritic cell-T cell interactions in the p*
    Mariana Kaplan; Fiscal Year: 2006
    ..These could lead into the development of novel therapeutic interventions designed to reverse these abnormalities and abrogate or block the onset and/or severity of this disease. ..
  13. IMMUNOPATHOGENESIS OF ACUTE HIV-1 INFECTION
    Elizabeth Connick; Fiscal Year: 2007
    ..abstract_text> ..
  14. Mucosal and Peripheral NK-T Cells in HIV Infection
    Otto Yang; Fiscal Year: 2007
    ..Exploring NK-T cells in HIV-1 infection could there have a high impact on the development of new strategies in prevention and treatment. [unreadable] [unreadable]..
  15. Immune Correlates of HSV-2 Disease Severity
    David Koelle; Fiscal Year: 2007
    ....
  16. Mechanisms of Leukocyte Recruitment During IPS After BMT
    Kenneth Cooke; Fiscal Year: 2007
    ..lymphocytes --> days 7-21 SA2: CXCR3 / IP-10 (CXCL10) and Mig (CXCL9) SA3: CCR2: / MCP-1 (CCL2) --> monocytes/macrophages --> days 14-28 SA4:CXCR2 / KC and MIP-2 (CXCL1) --> neutrophils --> days 21 to 42 ..
  17. Vector-based Generation of Monoclonal Antibodies against the CB2 Receptor
    Michael Roth; Fiscal Year: 2007
    ..This work has the capacity to significantly accelerate cannabinoid research. [unreadable] [unreadable] [unreadable]..
  18. AFP-Based Immunotherapy for Hepatocellular Carcinoma
    Antoni Ribas; Fiscal Year: 2006
    ..In summary, I propose a clinically-oriented research program that translates this original work into novel, evidence-based immnunotherapy trials for hepatocellular carcinoma. ..
  19. Mechanisms of T-cell induced-APC cytotoxicity in lupus
    Mariana Kaplan; Fiscal Year: 2006
    ..The sponsor and the institution are committed to contributing protected time, career development and resources to the applicant. ..
  20. ALABAMA/ZAMBIA HIV VACCINE CLINICAL TRIALS UNIT (HVTU)
    Paul Goepfert; Fiscal Year: 2006
    ..Recently, the Alabama clinic enrolled 125 higher-risk gay men in <5 months in the first US phase III trial. Plans for utilizing these cohorts and others are described. ..
  21. Anthrax DNA vaccine for non-invasive immunization on skin
    Zhengrong Cui; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  22. Conference-Safeguarding Adult/Pediatric Stem Cell Donors
    Edward Snyder; Fiscal Year: 2004
    ..This Symposium would address the need for such Standards and stimulate the process. AIM 3 will be to publish a summary of the Symposium in a nationally recognized Journal. ..
  23. Improving Gastrointestinal Recovery after Radiation
    George Georges; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  24. RHESUS MACAQUE MODEL OF TUBERCULOSIS
    DAVID LEWINSOHN; Fiscal Year: 2001
    ..g., CT scans, quantitative culture of mycobacteria from BAL fluids and liver biopsies) which correlate with survival. ..
  25. CYTOKINES AS PREDICTORS OF DISEASE PROGRESSION IN SCLERODERMA LUNG DISEASE
    Michael Roth; Fiscal Year: 2009
    ..It will also allow us to develop biologic markers and predictors of disease progression and to determine which patients would benefit most from treatment with cytotoxic therapy. ..
  26. THERAPY FOR AIDS CENTRAL NERVOUS SYSTEM LYMPHOMA
    Michael Caligiuri; Fiscal Year: 2002
    ..The limitations of the animal model preclude its use for studying the pathogenesis of AIDS PCNSL. ..
  27. Vaccinia: T-cell immunity and homing to skin
    David Koelle; Fiscal Year: 2009
    ..Our data will assist in evaluating the immunogenicity of these constructs. ..
  28. NK CELL ACTIVITY AND IBS ACROSS THE MENSTRUAL CYCLE
    Sandra Motzer; Fiscal Year: 2002
    ..abstract_text> ..
  29. Schistosome Calpain as a Vaccine Candidates
    Afzal Siddiqui; Fiscal Year: 2002
    ..Of greater importance, these studies should provide new insight into immune mechanisms which can damage worm parasites and establish a model by which to subsequently study the effector mechanisms. ..
  30. CHARACTERIZATION OF HUMAN 2B4 AND 2B4 GENE KNOCKOUT MICE
    PORUNELLOOR MATHEW; Fiscal Year: 2004
    ..The experiments proposed in this project should provide a clearer understanding of the role of 2B4 in modulating various functions of human NK cells and may reveal new insights and open new avenues for cancer immunotherapy. ..
  31. CD8+TCell Antiretroviral Mechanisms in Lymphoid Tissue
    Elizabeth Connick; Fiscal Year: 2003
    ..These studies could provide important insight into the immunopathogenesis of HIV-1 infection and suggest alternative targets for immune therapies. ..
  32. IL-15 Characterization Through Experimental Immunology
    Michael Caligiuri; Fiscal Year: 2009
    ..abstract_text> ..
  33. DONOR SPECIFIC IMMUNOSUPPRESSION TO TRACHEAL ALLOGRAFTS
    Eric Genden; Fiscal Year: 2004
    ..Specific Aim II will focus on alternative methods of inducing donor specific tolerance to tracheal allografts. Specific Aim III will focus on the behavior of the tracheal allograft and its affect upon the established state of tolerance. ..
  34. Innate-like hepatic CD1d-reative NKT cells:Liver Disease
    Mark Exley; Fiscal Year: 2008
    ..I. on acute HCV infection in chimpanzees, as well as on liver immunology and on 'NKT' cells in general. ..
  35. B7-DC Cross-linking antibody immunotherapy
    Svetomir Markovic; Fiscal Year: 2008
    ..If successful, this "proof-of-principle" clinical trial will justify further development of B7-DC XAb as a cancer therapeutic with potential universal application for all malignant disorders. [unreadable] [unreadable] [unreadable]..
  36. A tri-antigen nasal anthrax vaccine against toxins and vegetative bacilli
    Zhengrong Cui; Fiscal Year: 2008
    ..The same vaccine or its modified version can also be used in agricultural industries to vaccinate cattle. [unreadable] [unreadable] [unreadable]..
  37. Characterization of a vaccine against Chlamydia trachomatis
    RHEA COLER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  38. Stem Cell Quality Assays: Correlation with Aging/Health
    JOHN GRAHAM SHARP; Fiscal Year: 2010
    ..This research will validate laboratory assays of stem cell compartment quality which can then be applied to clinical situations and may reveal blood cytokine assays with surrogate predictive value. ..
  39. Influence of Lactation on Postpartum Stress and Immunity
    MAUREEN EDITH GROER; Fiscal Year: 2010
    ..The immunology and endocrinology of the postpartum provides a lens through which to study autoimmune disease, as the postpartum is a critical period for development or exacerbation of many autoimmune diseases. ..
  40. MECHANISM OF RETINAL DAMAGE IN AUTOIMMUNE RETINOPATHY
    Grazyna Adamus; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  41. Immunosupression-Resistant Gene Modified Donor T Cells
    George Georges; Fiscal Year: 2009
    ..Results from these studies have the strong potential to be directly translated to future gene therapy clinical trials. ..
  42. Exercise & influenza immunity:Psychoneuroendocrine Model
    Marian Kohut; Fiscal Year: 2004
    ..The findings from this study may have relevance from both a clinical and basic research perspective, if moderate exercise is associated with enhanced anti-influenza immunity and improved protection from infection. ..
  43. THE ROLE OF ALPHA-DEFENSINS IN CYSTIC FIBROSIS LUNG DISE
    L Spencer; Fiscal Year: 2004
    ..Following completion of this training and research, my long-term career interest will remain in the area of pathogenesis and applied clinical intervention of neutrophil-mediated pulmonary disease in pediatric patients. ..
  44. CMV DISRUPTION OF CONSTITUTIVE MHC CLASS II
    W Waldman; Fiscal Year: 2004
    ....
  45. EIAV Control by High Avidity Rev-Specific CTL Clones
    ROBERT MEALEY; Fiscal Year: 2005
    ..The information obtained from the proposed studies should have implications for HIV-1 immunotherapy and vaccine design, where experiments of this type may be more difficult. ..
  46. CD8+ T cell cytokine production in HIV-1 infection
    Paul Goepfert; Fiscal Year: 2005
    ..An HIV-1 vaccine would also be enhanced by identifying CD8+ T cell epitopes that portend a favorable prognosis and are difficult for the virus to mutate (specific aims 2 and 3). ..
  47. Gene Modified Donor T Cell Infusion into Mixed Chimeras
    George Georges; Fiscal Year: 2003
    ..If successful, this study could translate into improved control of the GVH reaction and improved survival after allogeneic HCT. ..
  48. ANTIVIRAL ACTIVITY OF THE IMMUNOSUPPRESSANT LEFLUNOMIDE
    W Waldman; Fiscal Year: 2004
    ....
  49. EARLY STAGES OF INFECTION BY NONENVELOPED VIRUSES
    Marie Chow; Fiscal Year: 2002
    ..It is aticipated that the studies will provide the biochemical and biophysical make-up of the channel structure and allow the applicants to build a general model of the early stages of infection by nonenveloped viruses. ..
  50. The regulation of tumor immunity by NKT cells
    Mark Smyth; Fiscal Year: 2007
    ..This proposal focuses clearly on a pivotal immune control mechanism that will influence many current and future immunotherapies. ..
  51. Recombinant T-cell Receptor Ligands for Treatment of Uveitis
    Grazyna Adamus; Fiscal Year: 2006
    ..To determine an effective treatment regimen for clinical rat AAU using RTLs. 2. To determine the abilities of RTLs to inhibit recurrent anterior and posterior uveitis in Lewis rats. [unreadable] [unreadable] [unreadable] [unreadable]..
  52. REGULATION OF HUMAN T-CELL IMMUNITY TO HCV ANTIGENS
    DAVID ECKELS; Fiscal Year: 2009
    ..abstract_text> ..
  53. HSV-SERONEGATIVE PERSONS WITH AQUIRED IMMUNITY TO HSV
    Christine Posavad; Fiscal Year: 2002
    ..abstract_text> ..
  54. HIV li-Key/Peptide Vaccine
    Minzhen Xu; Fiscal Year: 2002
    ..This entirely synthetic vaccine can be manufactured in a large scale and be given to those at high risk of HIV infection. ..
  55. li Suppression-enhanced HIV DNA Vaccine
    Minzhen Xu; Fiscal Year: 2003
    ..In a Phase II program Ii-RGC/gp120 DNA vaccines will be tested in non-human primates for the induction of a protective immune response against pathogenic HIV infection. ..
  56. HIGH AFFINITY AGONISTS OF HIV1 DEPENDENT FUSION
    Sylvie Blondelle; Fiscal Year: 2001
    ..Furthermore, studies toward the understanding of the mechanisms of action of these antagonists will be initiated. ..
  57. ANTITUMOR IMMUNITY INDUCED BY FUSION CELLS VACCINATION
    Jianlin Gong; Fiscal Year: 2003
    ..3). To assess the effectiveness of fusion cell vaccines in the prevention and treatment of spontaneous breast carcinoma in transgenic mice. ..
  58. EIAV Control by DNA Vaccine-Induced CTL
    ROBERT MEALEY; Fiscal Year: 2006
    ..The information obtained from the proposed studies should have implications for HIV-1 vaccine design, where experiments of this type may be more difficult. [unreadable] [unreadable]..
  59. Human Resistance to Herpes Simplex Virus Infections
    Christine Posavad; Fiscal Year: 2005
    ..The data generated will be relevant to designing and evaluating strategies for HSV-2 preventative vaccines and immunotherapy. ..
  60. Facilitating Cells for Fetal Hematopoietic Transplant
    Laurence Shields; Fiscal Year: 2005
    ..Future directions will be towards the use of this model to explore the role and function of developmentally regulated adhesion molecules and their receptors as they relate to fetal hematopoietic transplantation. ..
  61. The Role of CD4 on Natural Killer Cells
    HELENE BERNSTEIN; Fiscal Year: 2006
    ....
  62. IMMUNOREGULATORY EFFECTS OF MUTATION IN HCV NS3 ANTIGEN
    DAVID ECKELS; Fiscal Year: 2005
    ..abstract_text> ..
  63. Interaction of Yersinia with dendritic cells
    Igor Brodsky; Fiscal Year: 2007
    ..This knowledge will be important in the development of future strategies against human pathogens. ..
  64. T Cell Reactive Epitopes of Shrimp Tropomyosin
    Gerald Gleich; Fiscal Year: 2008
    ..Knowledge of the T-cell epitopes of shrimp tropomyosin will permit production of a vaccine and thus set the stage for a trial of T-cell epitope peptide therapy in allergy to shrimp. [unreadable] [unreadable] [unreadable]..
  65. Antibody in Defense Against Neonatal Candida Infections
    JOSEPH BLISS; Fiscal Year: 2008
    ..As such, he will be in an ideal position to orchestrate the translation of basic science to the benefit of patients. ..