epitope mapping

Summary

Summary: Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.

Top Publications

  1. ncbi Prediction of continuous B-cell epitopes in an antigen using recurrent neural network
    Sudipto Saha
    Institute of Microbial Technology, Chandigarh, India
    Proteins 65:40-8. 2006
  2. pmc Predicting linear B-cell epitopes using string kernels
    Yasser El-Manzalawy
    Artificial Intelligence Laboratory, Iowa State University, Ames, IA 50010, USA
    J Mol Recognit 21:243-55. 2008
  3. pmc Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus
    Theodore Oliphant
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, Missouri 63110, USA
    Nat Med 11:522-30. 2005
  4. pmc Prediction of residues in discontinuous B-cell epitopes using protein 3D structures
    Pernille Haste Andersen
    Center for Biological Sequence Analysis, BioCentrum, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Protein Sci 15:2558-67. 2006
  5. ncbi Structural basis of West Nile virus neutralization by a therapeutic antibody
    Grant E Nybakken
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 437:764-9. 2005
  6. ncbi The SPOT-synthesis technique. Synthetic peptide arrays on membrane supports--principles and applications
    Ronald Frank
    AG Molecular Recognition, German Research Centre for Biotechnology GBF, Mascheroder Weg 1, D 38124 Brunswick, Germany
    J Immunol Methods 267:13-26. 2002
  7. pmc ElliPro: a new structure-based tool for the prediction of antibody epitopes
    Julia Ponomarenko
    San Diego Supercomputer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    BMC Bioinformatics 9:514. 2008
  8. ncbi PEPITO: improved discontinuous B-cell epitope prediction using multiple distance thresholds and half sphere exposure
    Michael J Sweredoski
    Department of Computer Science and Institute for Genomics and Bioinformatics, University of California, Irvine, California 92697 3435, USA
    Bioinformatics 24:1459-60. 2008
  9. ncbi A consensus epitope prediction approach identifies the breadth of murine T(CD8+)-cell responses to vaccinia virus
    Magdalini Moutaftsi
    La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill Street, Suite 326, San Diego, California 92109, USA
    Nat Biotechnol 24:817-9. 2006
  10. pmc SEPPA: a computational server for spatial epitope prediction of protein antigens
    Jing Sun
    Department of Biomedical Engineering, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China
    Nucleic Acids Res 37:W612-6. 2009

Detail Information

Publications368 found, 100 shown here

  1. ncbi Prediction of continuous B-cell epitopes in an antigen using recurrent neural network
    Sudipto Saha
    Institute of Microbial Technology, Chandigarh, India
    Proteins 65:40-8. 2006
    ..The length of the peptide is also important in the prediction of B-cell epitopes from antigenic sequences. The webserver ABCpred is freely available at www.imtech.res.in/raghava/abcpred/...
  2. pmc Predicting linear B-cell epitopes using string kernels
    Yasser El-Manzalawy
    Artificial Intelligence Laboratory, Iowa State University, Ames, IA 50010, USA
    J Mol Recognit 21:243-55. 2008
    ..Our homology-reduced data set and implementations of BCPred as well as the APP method are publicly available through our web-based server, BCPREDS, at: http://ailab.cs.iastate.edu/bcpreds/...
  3. pmc Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus
    Theodore Oliphant
    Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, Box 8051, St Louis, Missouri 63110, USA
    Nat Med 11:522-30. 2005
    ..In postexposure therapeutic trials in mice, a single dose of humanized E16 protected mice against WNV-induced mortality, and may therefore be a viable treatment option against WNV infection in humans...
  4. pmc Prediction of residues in discontinuous B-cell epitopes using protein 3D structures
    Pernille Haste Andersen
    Center for Biological Sequence Analysis, BioCentrum, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Protein Sci 15:2558-67. 2006
    ..0% of residues located in discontinuous epitopes. Predictions by the DiscoTope method can guide experimental epitope mapping in both rational vaccine design and development of diagnostic tools, and may lead to more efficient epitope ..
  5. ncbi Structural basis of West Nile virus neutralization by a therapeutic antibody
    Grant E Nybakken
    Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 437:764-9. 2005
    ..Collectively, our results suggest that a vaccine strategy targeting the dominant DIII epitope may elicit safe and effective immune responses against flaviviral diseases...
  6. ncbi The SPOT-synthesis technique. Synthetic peptide arrays on membrane supports--principles and applications
    Ronald Frank
    AG Molecular Recognition, German Research Centre for Biotechnology GBF, Mascheroder Weg 1, D 38124 Brunswick, Germany
    J Immunol Methods 267:13-26. 2002
    ..Therefore, peptide arrays prepared by the SPOT-technique became quite popular tools for studying numerous aspects of molecular recognition, particularly in the field of molecular immunology...
  7. pmc ElliPro: a new structure-based tool for the prediction of antibody epitopes
    Julia Ponomarenko
    San Diego Supercomputer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    BMC Bioinformatics 9:514. 2008
    ..The aim of this work was to implement that method as a web-tool and evaluate its performance on discontinuous epitopes known from the structures of antibody-protein complexes...
  8. ncbi PEPITO: improved discontinuous B-cell epitope prediction using multiple distance thresholds and half sphere exposure
    Michael J Sweredoski
    Department of Computer Science and Institute for Genomics and Bioinformatics, University of California, Irvine, California 92697 3435, USA
    Bioinformatics 24:1459-60. 2008
    ..Up to 90% of B-cell epitopes are discontinuous in nature, yet most predictors focus on linear epitopes. Even when the tertiary structure of the antigen is available, the accurate prediction of B-cell epitopes remains challenging...
  9. ncbi A consensus epitope prediction approach identifies the breadth of murine T(CD8+)-cell responses to vaccinia virus
    Magdalini Moutaftsi
    La Jolla Institute for Allergy and Immunology, 3030 Bunker Hill Street, Suite 326, San Diego, California 92109, USA
    Nat Biotechnol 24:817-9. 2006
    ..This approach reveals the breadth of T-cell responses to vaccinia, a widely studied murine viral infection model, and may provide a tool for developing comprehensive antigenic maps of any complex pathogen...
  10. pmc SEPPA: a computational server for spatial epitope prediction of protein antigens
    Jing Sun
    Department of Biomedical Engineering, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China
    Nucleic Acids Res 37:W612-6. 2009
    ..The web server can be accessed at http://lifecenter.sgst.cn/seppa/index.php. Batch query is supported...
  11. pmc Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes
    Wayne D Crill
    Arbovirus Diseases Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, P O Box 2087, Fort Collins, CO 80522, USA
    J Virol 78:13975-86. 2004
    ..The third epitope consists of discontinuous residues that are structurally related to the strictly conserved tryptophan at dengue virus serotype 2 E-glycoprotein position 231...
  12. ncbi Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display
    Ginger Chao
    Department of Chemical Engineering, Massachusetts Institute of Technology, MIT 66 552, Cambridge, MA 02139, USA
    J Mol Biol 342:539-50. 2004
    Fine epitope mapping of therapeutically relevant monoclonal antibodies (mAbs) specific for the epidermal growth factor receptor (EGFR) was accomplished through random mutagenesis and yeast surface display...
  13. pmc Fine mapping of the interaction of neutralizing and nonneutralizing monoclonal antibodies with the CD4 binding site of human immunodeficiency virus type 1 gp120
    Ralph Pantophlet
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 77:642-58. 2003
    ..These reengineered gp120s are prospective immunogens that may prove capable of eliciting broadly neutralizing antibodies...
  14. pmc Antibody specificities associated with neutralization breadth in plasma from human immunodeficiency virus type 1 subtype C-infected blood donors
    Elin S Gray
    AIDS Virus Research Unit, National Institute for Communicable Diseases, Johannesburg, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
    J Virol 83:8925-37. 2009
    ....
  15. ncbi Epitope mapping and protective immunity elicited by adenovirus expressing the Leishmania amastigote specific A2 antigen: correlation with IFN-gamma and cytolytic activity by CD8+ T cells
    Daniela M Resende
    Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, MG, 31270 901, Brazil
    Vaccine 26:4585-93. 2008
    ..Finally, we demonstrated an association of A2-specific T cell responses and reduced parasitism in both liver and spleen from mice immunized with AdA2 and challenged with L. (L.) chagasi...
  16. pmc Human monoclonal antibody to hepatitis C virus E1 glycoprotein that blocks virus attachment and viral infectivity
    Zhen Yong Keck
    Department of Pathology, Stanford University School of Medicine, CA 94305, USA
    J Virol 78:7257-63. 2004
    ..Finally, H-111 blocks HCV-like particle binding to and HCV virion infection of target cells, suggesting the involvement of this epitope in virus binding and entry...
  17. ncbi Identification and characterization of a cross-neutralization epitope of Enterovirus 71
    Chia Chyi Liu
    Vaccine R and D Center, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan
    Vaccine 29:4362-72. 2011
    ..During the epitope mapping, a murine EV71 genotype- and strain-specific linear neutralization epitope VP1-43 was identified within ..
  18. ncbi Epitope mapping: the first step in developing epitope-based vaccines
    Jonathan M Gershoni
    Department of Cell Research and Immunology, Tel Aviv University, Tel Aviv, Israel
    BioDrugs 21:145-56. 2007
    ..In addition, computational combinatorial methods for epitope mapping are also useful...
  19. ncbi Characterization of neutralizing antibodies to West Nile virus
    Melissa D Sanchez
    Department of Microbiology, University of Pennsylvania, 225 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    Virology 336:70-82. 2005
    ..Interestingly, some non-neutralizing MAbs bound with high affinity to domains I or III, indicating that both affinity and the precise epitope recognized by an antibody are important determinants of WNV neutralization...
  20. pmc Structure and function analysis of therapeutic monoclonal antibodies against dengue virus type 2
    Soila Sukupolvi-Petty
    Department of Medicine, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, St Louis, MO 63110, USA
    J Virol 84:9227-39. 2010
    ..Overall, these studies define the structural and functional complexity of antibodies against DENV-2 with protective potential...
  21. ncbi Pepitope: epitope mapping from affinity-selected peptides
    Itay Mayrose
    Department of Cell Research and Immunology, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Bioinformatics 23:3244-6. 2007
    ..The server implements three different algorithms for epitope mapping: PepSurf, Mapitope, and a combination of the two...
  22. pmc Human combinatorial libraries yield rare antibodies that broadly neutralize hepatitis C virus
    Daniel X Johansson
    Department of Medicine, Center for Molecular Medicine, Karolinska University Hospital Solna, Karolinska Institutet, 171 76 Stockholm, Sweden
    Proc Natl Acad Sci U S A 104:16269-74. 2007
    ....
  23. ncbi Neutralizing antibodies generated during natural HIV-1 infection: good news for an HIV-1 vaccine?
    Leonidas Stamatatos
    1Seattle Biomedical Research Institute, Seattle, Washington, USA
    Nat Med 15:866-70. 2009
    ..A concerted effort of structure-based vaccine design will help guide the development of improved antibody-based vaccines for HIV-1...
  24. pmc Identification and characterization of broadly neutralizing human monoclonal antibodies directed against the E2 envelope glycoprotein of hepatitis C virus
    Teresa J Broering
    MassBiologics, University of Massachusetts Medical School, Boston, Massachusetts 02130, USA
    J Virol 83:12473-82. 2009
    ..b>Epitope mapping with mammalian and bacterially expressed proteins, as well as synthetic peptides, revealed that HuMAbs HCV1 ..
  25. pmc Hepatitis C virus epitope-specific neutralizing antibodies in Igs prepared from human plasma
    Pei Zhang
    Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:8449-54. 2007
    ....
  26. pmc Epitope mapping of the hemagglutinin molecule of a highly pathogenic H5N1 influenza virus by using monoclonal antibodies
    Nikolai V Kaverin
    D I Ivanovsky Institute of Virology, 123098 Moscow, Russia
    J Virol 81:12911-7. 2007
    ....
  27. pmc A broadly flavivirus cross-neutralizing monoclonal antibody that recognizes a novel epitope within the fusion loop of E protein
    Yong qiang Deng
    State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
    PLoS ONE 6:e16059. 2011
    ..These results define a novel broadly flavivirus cross-reactive mAb with highly neutralizing activity that can be further developed as a therapeutic agent against severe flavivirus infections in humans...
  28. ncbi Characterisation and epitope mapping of neutralising monoclonal antibodies to A24 Cruzeiro strain of FMDV
    M Mahapatra
    Institute for Animal Health, Pirbright Laboratory, Ash Road, Woking, Surrey GU24 0NF, UK
    Vet Microbiol 149:242-7. 2011
    ..This site is probably formed because of the interaction of the G-H loop with other residues in different structural proteins...
  29. pmc Mapping B-cell epitopes of hepatitis C virus E2 glycoprotein using human monoclonal antibodies from phage display libraries
    F Bugli
    Istituti di Microbiologia, , , 00168 Rome, Italy
    J Virol 75:9986-90. 2001
    ....
  30. ncbi Epitope identification and discovery using phage display libraries: applications in vaccine development and diagnostics
    Lin Fa Wang
    CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong 3220, Victoria, Australia
    Curr Drug Targets 5:1-15. 2004
    ..Instead, this review aims to focus on specific applications related to the discovery and identification of epitopes which have potential as vaccine candidates or can be used in disease diagnosis...
  31. ncbi Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica
    J T Roehrig
    Arbovirus Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
    Virology 246:317-28. 1998
    ..Domains A and B were spatially associated. As with tick-borne encephalitis virus, determination of domain C was more problematic; however, at least four epitopes had biochemical characteristics consistent with C-domain epitopes...
  32. pmc Factors associated with the development of cross-reactive neutralizing antibodies during human immunodeficiency virus type 1 infection
    D Noah Sather
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    J Virol 83:757-69. 2009
    ..Overall, our study indicates that more than one pathway leads to the development of broad cross-reactive neutralizing antibodies during HIV infection and that the virus continuously escapes their action...
  33. ncbi Characterization of an antigenic site that contains a dominant, type-specific neutralization determinant on the envelope protein domain III (ED3) of dengue 2 virus
    Gregory D Gromowski
    Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases, and Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555 0609, USA
    Virology 366:349-60. 2007
    ..Overall, the conserved antigenic site recognized by all seven MAbs is likely to be a dominant DENV-2 type-specific, neutralization determinant...
  34. pmc Humoral immune responses of dengue fever patients using epitope-specific serotype-2 virus-like particle antigens
    Wayne D Crill
    Arbovirus Diseases Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Service, Fort Collins, CO, USA
    PLoS ONE 4:e4991. 2009
    ....
  35. pmc Mapping a region of hepatitis C virus E2 that is responsible for escape from neutralizing antibodies and a core CD81-binding region that does not tolerate neutralization escape mutations
    Zhen Yong Keck
    Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Virol 85:10451-63. 2011
    ..b>Epitope mapping revealed contact residues for CBH-2 and HC-11 in two regions of the E2 glycoprotein, amino acids (aa) 425 to ..
  36. pmc Characterization of dengue virus complex-specific neutralizing epitopes on envelope protein domain III of dengue 2 virus
    Gregory D Gromowski
    Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas 77555 0609, USA
    J Virol 82:8828-37. 2008
    ....
  37. pmc In vitro selection of a neutralization-resistant hepatitis C virus escape mutant
    Meital Gal-Tanamy
    Center for Hepatitis Research, Institute for Human Infections and Immunity, and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555 1073, USA
    Proc Natl Acad Sci U S A 105:19450-5. 2008
    ....
  38. pmc Generation, characterization and epitope mapping of two neutralizing and protective human recombinant antibodies against influenza A H5N1 viruses
    Lina Sun
    State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
    PLoS ONE 4:e5476. 2009
    ....
  39. ncbi The crystal structure of human angiogenin in complex with an antitumor neutralizing antibody
    Gayatri B Chavali
    Department of Biology and Biochemistry, University of Bath, Claverton Down, BA2 7AY, Bath, United Kingdom
    Structure 11:875-85. 2003
    ..The ANG-Fab complex structure should be invaluable for designing maximally humanized versions of 26-2F for potential clinical use...
  40. pmc Structural analysis of B-cell epitopes in antibody:protein complexes
    Jens Vindahl Kringelum
    Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Kemitorvet, Building 208, DK 2800 Lyngby, Denmark
    Mol Immunol 53:24-34. 2013
    ..However experimental epitope mapping is highly cost-intensive and computer-aided methods do in general have moderate performance...
  41. pmc Development of a humanized antibody with high therapeutic potential against dengue virus type 2
    Pi Chun Li
    Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
    PLoS Negl Trop Dis 6:e1636. 2012
    ..Dengue virus (DENV) is a significant public health threat in tropical and subtropical regions of the world. A therapeutic antibody against the viral envelope (E) protein represents a promising immunotherapy for disease control...
  42. pmc Degenerate T-cell recognition of peptides on MHC molecules creates large holes in the T-cell repertoire
    Jorg J A Calis
    Theoretical Biology and Bioinformatics, Utrecht University, Utrecht, The Netherlands
    PLoS Comput Biol 8:e1002412. 2012
    ..Degenerate recognition of peptide-MHC-I complexes by T-cells thus creates large and potentially dangerous overlaps between self and nonself...
  43. pmc Mapping epitopes of the Plasmodium vivax Duffy binding protein with naturally acquired inhibitory antibodies
    Patchanee Chootong
    Global Health Infectious Disease Research Program, University of South Florida, Tampa, Florida, USA
    Infect Immun 78:1089-95. 2010
    ..These results represent an important advance in our understanding of part of blood-stage immunity to P. vivax and some of the specific targets for vaccine-elicited antibody protection...
  44. pmc Natural strain variation and antibody neutralization of dengue serotype 3 viruses
    Wahala M P B Wahala
    Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America
    PLoS Pathog 6:e1000821. 2010
    ..Our data demonstrate that the lateral ridge "type specific" epitope is not conserved between strains of DENV3. This variability should be considered when designing and evaluating DENV vaccines, especially those targeting EDIII...
  45. pmc Antibodies against West Nile Virus nonstructural protein NS1 prevent lethal infection through Fc gamma receptor-dependent and -independent mechanisms
    Kyung Min Chung
    Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Campus Box 8051, 660 S Euclid Avenue, St Louis, MO 63110, USA
    J Virol 80:1340-51. 2006
    ..Overall, our data suggest that distinct regions of NS1 can elicit protective humoral immunity against WNV through different mechanisms...
  46. pmc B cell epitope mapping and characterization of naturally acquired antibodies to the Plasmodium vivax merozoite surface protein-3α (PvMSP-3α) in malaria exposed individuals from Brazilian Amazon
    J C Lima-Junior
    Laboratory of Immunoparasitology, Institute Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil
    Vaccine 29:1801-11. 2011
    ..PvMSP3 specific antibodies (IgA, IgG and IgG subclass) to five recombinant proteins and the epitope mapping by Spot-synthesis technique to full-protein sequence of amino acids (15aa sequence with overlapping sequence ..
  47. pmc Human monoclonal antibodies against West Nile virus induced by natural infection neutralize at a postattachment step
    Matthew R Vogt
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Virol 83:6494-507. 2009
    ..CR4348 and CR4354 human MAbs neutralize infection at a postattachment step in the viral life cycle, likely by inhibiting acid-induced fusion within the endosome...
  48. ncbi Mutations of an antibody binding energy hot spot on domain III of the dengue 2 envelope glycoprotein exploited for neutralization escape
    Gregory D Gromowski
    Department of Pathology, Sealy Center for Vaccine Development, and Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555 0609, USA
    Virology 407:237-46. 2010
    ....
  49. pmc Identification of a broadly cross-reacting and neutralizing human monoclonal antibody directed against the hepatitis C virus E2 protein
    Mario Perotti
    Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, DIBIT2, Via Olgettina 60, 20132 Milano, Italia
    J Virol 82:1047-52. 2008
    ..Fab e137 was also able to inhibit cell culture-grown HCV (genotype 2a). These data indicate that broadly cross-reacting and cross-neutralizing antibodies are generated during HCV infection...
  50. ncbi The peanut allergy epidemic: allergen molecular characterisation and prospects for specific therapy
    Maria P de Leon
    Department of Immunology, Monash University, Melbourne, Victoria 3004, Australia
    Expert Rev Mol Med 9:1-18. 2007
    ..These include T-cell-epitope peptide and hypoallergenic mutant vaccines. Alternative routes of administration such as sublingual are also considered, and appropriate adjuvants for delivering effective treatments at these sites examined...
  51. pmc Epitope mapping of immunogenic and adhesive structures in repetitive domains of Mycoplasma bovis variable surface lipoproteins
    K Sachse
    Division 4, Federal Institute for Health Protection of Consumers and Veterinary Medicine BgVV, 07743 Jena, Germany
    Infect Immun 68:680-7. 2000
    ....
  52. pmc One-pot, mix-and-read peptide-MHC tetramers
    Christian Leisner
    Laboratories of Experimental Immunology, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 3:e1678. 2008
    ..Such cumbersome production protocols have limited dissemination and restricted availability of peptide-MHC tetramers effectively precluding large-scale screening strategies involving many different peptide-MHC tetramers...
  53. ncbi Designed ankyrin repeat protein binders for the crystallization of AcrB: plasticity of the dominant interface
    Nicole Monroe
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Struct Biol 174:269-81. 2011
    ..We observe that small variations in the interface can lead to differing behavior of the DARPins with regards to affinity, stoichiometry of the complexes and specificity for their target...
  54. pmc Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1
    A Trkola
    Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016, USA
    J Virol 70:1100-8. 1996
    ..consistent with this, antibodies able to block 2G12 binding to recombinant gp120 were not detected in significant quantities in 16 HIV-positive human serum samples...
  55. ncbi Epitope prediction based on random peptide library screening: benchmark dataset and prediction tools evaluation
    Pingping Sun
    Faculty of Chemistry, Northeast Normal University, Changchun 130024, China
    Molecules 16:4971-93. 2011
    ..Therefore finding the correlation between mimotopes and genuine epitope residues is still far from resolved and much larger dataset for mimotope-based epitope prediction is desirable...
  56. ncbi Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase
    Barbara Hjelm
    Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
    N Biotechnol 27:129-37. 2010
    ..An alanine-scanning experiment showed binding to four specific residues. The implications for the systematic analysis of antibody epitopes on the basis of these results are discussed...
  57. pmc Epitope mapping of HIV-specific CD8+ T cell responses by multiple immunological readouts reveals distinct specificities defined by function
    Meika Richmond
    Department of Medical Microbiology, University of Manitoba, H3440 1015 Arlington St, Winnipeg, MB R3E 3R2 Canada
    J Virol 85:1275-86. 2011
    ..Furthermore, the identification of epitopes that elicit polyfunctional responses reinforces the need for the comprehensive evaluation of HIV vaccine candidates, and these epitopes may represent novel targets for CMI-based vaccines...
  58. ncbi Asp159 is a critical core amino acid of an IgE-binding and cross-reactive epitope of a dust mite allergen Der f 7
    H Chou
    Department of Medical Research and Education, Taipei Veterans General Hospital, Shih Pai, Taipei 11217, Taiwan
    Mol Immunol 48:2130-4. 2011
    ..Results obtained from this study provide more information on molecular and structural features related to allergenicity, underlying basis of IgE cross-reactivity between allergens, and in designing safer immunotherapy...
  59. ncbi Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity
    Sabine Costagliola
    Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire, ULB, 808 Lennik Street, B 1070 Brussels, Belgium
    Mol Endocrinol 18:3020-34. 2004
    ..Delineation of the conformational epitopes of these stimulating antibodies opens the way to the identification of the molecular mechanisms implicated in the activation of the TSHr...
  60. pmc Potent and broad neutralization of HIV-1 subtype C by plasma antibodies targeting a quaternary epitope including residues in the V2 loop
    Penny L Moore
    National Institute for Communicable Diseases of the NHLS, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
    J Virol 85:3128-41. 2011
    ....
  61. ncbi Structure-function analysis of the integrin beta 7 subunit: identification of domains involved in adhesion to MAdCAM-1
    M Tidswell
    Lung Biology Center, Department of Medicine, University of California, San Francisco 94143, USA
    J Immunol 159:1497-505. 1997
    ..One blocking and five other Abs mapped to other regions (amino acids 387-725). We conclude that a MIDAS-like domain serves a critical role in beta 7 integrin-mediated adhesion...
  62. pmc Protective immune responses to a recombinant adenovirus type 35 tuberculosis vaccine in two mouse strains: CD4 and CD8 T-cell epitope mapping and role of gamma interferon
    Katarina Radosevic
    Crucell Holland BV, Archimedesweg 4 6, 2333 CN Leiden, The Netherlands
    Infect Immun 75:4105-15. 2007
    ..b>Epitope mapping analysis in these mouse strains showed that the major T-cell epitopes are conserved in the artificial fusion ..
  63. ncbi Lupus antibodies to the HMGB1 chromosomal protein: epitope mapping and association with disease activity
    Ayako Hayashi
    Division of Rheumatology and Allergy, Department of Internal Medicine, St Marianna University School of Medicine, Miyamae ku, Kawasaki, Japan
    Mod Rheumatol 19:283-92. 2009
    ..Through epitope mapping, we found that multiple HMGB1 epitopes were recognised in SLE sera, with the major epitope mapping to box A...
  64. ncbi Characterization of epitopes for neutralizing monoclonal antibodies to classical swine fever virus E2 and Erns using phage-displayed random peptide library
    F Zhang
    CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Australia
    Arch Virol 151:37-54. 2006
    ..The detailed mapping data for these neutralizing epitopes will be useful for development of improved diagnostic tests and perhaps a peptide-based vaccine for this important swine disease...
  65. pmc Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2
    Porntippa Lekcharoensuk
    Department of Microbiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
    J Virol 78:8135-45. 2004
    ....
  66. pmc Folding domains within the ricin toxin A subunit as targets of protective antibodies
    Joanne M O'Hara
    Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
    Vaccine 28:7035-46. 2010
    ..These data offer insights into the immunodominant and structural determinants on RTA that give rise to protective immunity, and for the first time provide an immunological rationale for ricin vaccine design...
  67. ncbi T cell epitope: friend or foe? Immunogenicity of biologics in context
    Constanze A Weber
    EpiVax, Providence, RI 02903, USA
    Adv Drug Deliv Rev 61:965-76. 2009
    ..In this review, we address the application of computational tools for preclinical immunogenicity assessment, the implication of the discovery of regulatory T cell epitopes, and experimental validation of those assessments...
  68. pmc EPSVR and EPMeta: prediction of antigenic epitopes using support vector regression and multiple server results
    Shide Liang
    Jacobs University Bremen, Germany
    BMC Bioinformatics 11:381. 2010
    ..The small number of structures for antigen-antibody complexes limits the development of reliable discontinuous epitope prediction methods and an unbiased benchmark to evaluate developed methods...
  69. pmc Adjuvant-dependent modulation of Th1 and Th2 responses to immunization with beta-amyloid
    David H Cribbs
    Institute for Brain Aging and Dementia, University of California Irvine, Irvine, CA 92697 4540, USA
    Int Immunol 15:505-14. 2003
    ....
  70. pmc Protective immunity to ricin toxin conferred by antibodies against the toxin's binding subunit (RTB)
    Anastasiya Yermakova
    Department of Biomedical Sciences, University at Albany School of Public Health, Albany, NY 12201, USA
    Vaccine 29:7925-35. 2011
    ..These data raise the possibility of using specific RTB sub-domains, rather than RTB itself, as antigens to more efficiently elicit neutralizing antibodies and protective immunity against ricin...
  71. ncbi Structure-based antigen design: a strategy for next generation vaccines
    Philip R Dormitzer
    Novartis Vaccines and Diagnostics, Inc, 350 Massachusetts Ave, Cambridge, MA 02139, USA
    Trends Biotechnol 26:659-67. 2008
    ..Understanding the structural basis for immunogenicity and immunodominance will allow us to improve vaccine efficacy and broaden the range of vaccine-preventable diseases...
  72. ncbi Epitope mapping of antibodies using bacterial surface display
    Johan Rockberg
    Department of Molecular Biotechnology, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, SE 106 91 Stockholm, Sweden
    Nat Methods 5:1039-45. 2008
    ..Our approach shows a path forward for systematic validation of antibodies for epitope specificity and cross-reactivity on a whole-proteome level...
  73. ncbi Neutralizing monoclonal antibody against anthrax lethal factor inhibits intoxication in a mouse model
    Ping Zhao
    Laboratory of Antibody Technology, Van Andel Research Institute, Grand Rapids, MI 49503, USA
    Hum Antibodies 12:129-35. 2003
    ..These small peptides compete with LF to bind LF8. Further investigation is undergoing to test the potential application of these peptides to the clinical treatment of anthrax infection by blocking LeTx formation...
  74. ncbi Nuclear magnetic resonance structure-based epitope mapping and modulation of dust mite group 13 allergen as a hypoallergen
    Siew Leong Chan
    Department of Biological Sciences, National University of Singapore, Singapore 117543
    J Immunol 176:4852-60. 2006
    ..Findings in this study imply the importance of surface charged residues on IgE binding and allergenicity of an allergen, as was also demonstrated in other major allergens studied...
  75. ncbi Nuclear magnetic resonance structure and IgE epitopes of Blo t 5, a major dust mite allergen
    Siew Leong Chan
    Department of Biological Sciences, National University of Singapore, Singapore, Singapore
    J Immunol 181:2586-96. 2008
    ..Findings in this study further stressed the importance of surface-charged residues on IgE binding and have implications in the cross-reactivity and use of Blo t 5 mutants as a hypoallergen for immunotherapy...
  76. ncbi Comparison of overlapping peptide sets for detection of antiviral CD8 and CD4 T cell responses
    Rika Draenert
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, MA 02129, USA
    J Immunol Methods 275:19-29. 2003
    ..We conclude that peptides ranging from 15 to 20 amino acids yield similar results in IFN-gamma-based Elispot and ICS assays, and that all are likely to underestimate the true breadth of responses to a given reference strain of virus...
  77. pmc Epitope determinants of a chimpanzee Fab antibody that efficiently cross-neutralizes dengue type 1 and type 2 viruses map to inside and in close proximity to fusion loop of the dengue type 2 virus envelope glycoprotein
    Ana P Goncalvez
    Molecular Viral Biology Section, Laboratory of Infectious Diseases, NIAID, NIH, Building 50, Room 6349, 50 South Dr, MSC 8009, Bethesda, MD 20892, USA
    J Virol 78:12919-28. 2004
    ..From the locations of these amino acids, Fab 1A5 appears to recognize a novel epitope that has not been mapped before with a flavivirus monoclonal antibody...
  78. ncbi Functional reconstruction and synthetic mimicry of a conformational epitope using CLIPS technology
    Peter Timmerman
    Pepscan Therapeutics B V, PO Box 2098, 8203 AB Lelystad, The Netherlands
    J Mol Recognit 20:283-99. 2007
    ..Design of the CLIPS-peptides was entirely based on epitope mapping studies with two neutralizing anti-hFSH mAbs...
  79. pmc The mannose-dependent epitope for neutralizing antibody 2G12 on human immunodeficiency virus type 1 glycoprotein gp120
    Rogier W Sanders
    Dept of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Virol 76:7293-305. 2002
    ....
  80. ncbi Enhancing biosynthesis and secretion of premembrane and envelope proteins by the chimeric plasmid of dengue virus type 2 and Japanese encephalitis virus
    Gwong Jen J Chang
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Service, Post Office Box 2087, Fort Collins, CO 80522, USA
    Virology 306:170-80. 2003
    ..Eleven of twelve 2-day-old neonatal mice, derived from a pCB8D2-2J-2-9-1 immunized female mouse, survived intraperitoneal challenge of DEN-2 New Guinea C virus...
  81. ncbi Human monoclonal antibodies that react with the E2 glycoprotein of hepatitis C virus and possess neutralizing activity
    Darren J Schofield
    Hepatitis Viruses Section, Laboratory of Infectious Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892 8009, USA
    Hepatology 42:1055-62. 2005
    ..In conclusion, one or more of these monoclonal antibodies may be useful in preventing infections by HCV belonging to genotype 1 or 2, the most medically important genotypes worldwide...
  82. pmc Pep-3D-Search: a method for B-cell epitope prediction based on mimotope analysis
    Yan Xin Huang
    Institute of Genetics and Cytology, Northeast Normal University, Changchun, PR China
    BMC Bioinformatics 9:538. 2008
    ..Though some algorithms based on mimotope analysis have been proposed, the precise localization of the interaction site mimicked by the mimotopes is still a challenging task...
  83. ncbi Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes
    D Mahe
    Agence Francaise de Securite Sanitaire des Aliments, Laboratoire de Biologie Moleculaire, BP 53, 22440 Ploufragan, France
    J Gen Virol 81:1815-24. 2000
    ..However, only some ORF2 peptides proved to be immunorelevant epitopes for virus type discrimination. The potential use of ORF2-derived antigens as diagnostic tools is demonstrated...
  84. pmc Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display
    R Levy
    Department of Anesthesia and Pharmaceutical Chemistry, University of California, San Francisco, San Francisco General Hospital, CA 94110, USA
    J Mol Biol 365:196-210. 2007
    ..purify each domain, and it should prove possible to display domains of other BoNT subtypes and serotypes for epitope mapping. Using a library of yeast-displayed BoNT/A binding domain (H(C)) mutants and selecting for loss of binding, ..
  85. pmc Comprehensive analysis of T cell epitope discovery strategies using 17DD yellow fever virus structural proteins and BALB/c (H2d) mice model
    Milton Maciel
    Johns Hopkins University, School of Medicine, Pharmacology Department, Baltimore, USA
    Virology 378:105-17. 2008
    ..The results allowed the characterization of 2 MHC class I and 17 class II epitopes in the envelope protein of the YF virus in BALB/c (H2d) mice...
  86. pmc Analysis of neutralization specificities in polyclonal sera derived from human immunodeficiency virus type 1-infected individuals
    Yuxing Li
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892 3005, USA
    J Virol 83:1045-59. 2009
    ..These data allow a more detailed understanding of the humoral responses to the HIV-1 Env protein and provide insights regarding the most relevant targets for HIV-1 vaccine design...
  87. pmc The development of therapeutic antibodies that neutralize homologous and heterologous genotypes of dengue virus type 1
    Bimmi Shrestha
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS Pathog 6:e1000823. 2010
    ..Using epitope mapping and X-ray crystallographic analyses, we localized the neutralizing determinants for the strongly inhibitory ..
  88. pmc Viral and cellular determinants of the hepatitis C virus envelope-heparan sulfate interaction
    Heidi Barth
    Department of Medicine II, University of Freiburg, Hugstetter Strasse 55, D 79106 Freiburg, Germany
    J Virol 80:10579-90. 2006
    ..Mapping of viral and cellular determinants of HCV-HS interaction sets the stage for the development of novel HS-based antiviral strategies targeting viral attachment and entry...
  89. pmc Coping with viral diversity in HIV vaccine design
    David C Nickle
    Department of Microbiology, University of Washington School of Medicine, Seattle, Washington, United States of America
    PLoS Comput Biol 3:e75. 2007
    ..These immunogens should elicit immune responses against high-frequency viral strains as well as against most mutant forms of the virus...
  90. ncbi Hybrid papillomavirus L1 molecules assemble into virus-like particles that reconstitute conformational epitopes and induce neutralizing antibodies to distinct HPV types
    N D Christensen
    The Jake Gittlen Cancer Research Institute, Pathology Department, Milton S Hershey Medical Center, 500 University Drive, Hershey, PA 17033, USA
    Virology 291:324-34. 2001
    ....
  91. pmc Antigenic and mutational analyses of herpes simplex virus glycoprotein B reveal four functional regions
    Florent C Bender
    Department of Microbiology, University of Pennsylvania, School of Dental Medicine, 240 S 40th Street, Levy Bldg R217, Philadelphia, PA 19104, and Children s Hospital, Boston, MA 02115, USA
    J Virol 81:3827-41. 2007
    ..Our data suggest that multiple domains are critical for gB function...
  92. ncbi Phage display for epitope determination: a paradigm for identifying receptor-ligand interactions
    Merrill J Rowley
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
    Biotechnol Annu Rev 10:151-88. 2004
    ....
  93. ncbi Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries
    J B Burritt
    Department of Microbiology, Montana State University, Bozeman 59717, USA
    J Biol Chem 270:16974-80. 1995
    ..These results suggest the 181GGPQVNPI188 segment of p22phox is accessible on its intracellular surface, but the 382PKIAVDGP389 region on gp91phox is not accessible to antibody, and probably not on the protein surface...
  94. ncbi Mapping of SOMU1 and M1 epitopes on the apomucin encoded by the 5' end of the MUC5AC gene
    S Nollet
    Mucin Immunochemistry Laboratory, INSERM U 482, Paris, France
    Hybrid Hybridomics 23:93-9. 2004
    ..Such antibodies are useful tools for studying the biosynthesis, polymerization, and degradation of mucin...
  95. pmc Predicting flexible length linear B-cell epitopes
    Yasser El-Manzalawy
    Artificial Intelligence Laboratory, Iowa State University, Ames, IA 50010, USA
    Comput Syst Bioinformatics Conf 7:121-32. 2008
    ..An implementation of FBCPred and the datasets used in this study are publicly available through our linear B-cell epitope prediction server, BCPREDS, at: http://ailab.cs.iastate.edu/bcpreds/...
  96. ncbi HLArestrictor--a tool for patient-specific predictions of HLA restriction elements and optimal epitopes within peptides
    Malene Erup Larsen
    Center for Biological Sequence Analysis, DTU Systems Biology, Building 208, Technical University of Denmark, DK 2800, Lyngby, Denmark
    Immunogenetics 63:43-55. 2011
    ..Thus, HLArestrictor should be a valuable tool in any T cell epitope discovery process aimed at identifying new epitopes from infectious diseases and other disease models...
  97. pmc Molecular determinants of T cell epitope recognition to the common Timothy grass allergen
    Carla Oseroff
    La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
    J Immunol 185:943-55. 2010
    ..Consistent with this notion, different Phl p Ags were associated with distinct patterns of IL-5, IFN-gamma, IL-10, and IL-17 production...
  98. ncbi Beta 2-microglobulin-free HLA class I heavy chain epitope mimicry by monoclonal antibody HC-10-specific peptide
    Federico Perosa
    Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, University of Bari Medical School, Bari, Italy
    J Immunol 171:1918-26. 2003
    ..This information contributes to our understanding of the molecular basis of the antigenic profiles of beta(2)m-free and beta(2)m-associated HLA-I HC and may serve to develop active specific immunotherapy of spondyloarthropathies...
  99. pmc The integrin alpha-subunit leg extends at a Ca2+-dependent epitope in the thigh/genu interface upon activation
    Can Xie
    CBR Institute for Biomedical Research and Departments of Pathology and Anesthesia, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:15422-7. 2004
    ..Together with inspection of the alphaV structure, this result suggests that the genu/calf-1 interface is maintained in integrin activation, and that extension occurs by a rearrangement at the thigh/genu interface...
  100. pmc Relevant B cell epitopes in allergic disease
    Anna Pomes
    INDOOR Biotechnologies, Inc, Charlottesville, VA, USA
    Int Arch Allergy Immunol 152:1-11. 2010
    ..Starting with the molecular cloning and expression of allergens, and with the advent of X-ray crystallography and nuclear magnetic resonance techniques, we have been able to visualize conformational epitopes on allergens...
  101. pmc Exploiting antigenic diversity for vaccine design: the chlamydia ArtJ paradigm
    Marco Soriani
    Novartis Vaccines, Via Fiorentina 1, 53100 Siena, Italy
    J Biol Chem 285:30126-38. 2010
    ..Experimental epitope mapping and computational analysis of physicochemical determinants of antibody recognition revealed that immunogenic ..

Research Grants62

  1. ETIOLOGY AND PATHOGENESIS OF PEMPHIGUS
    Luis A Diaz; Fiscal Year: 2013
    ..These scFv antibodies will be tested for pathogenicity and used for more precise epitope mapping. Further, "revertant" monoclonal antibodies will be generated and tested for reactivity with Dsg1 and LJM11 ..
  2. Immuno-epidemiological epitope mapping of a blood stage malaria vaccine antigen
    Christopher V Plowe; Fiscal Year: 2013
    ....
  3. Antibody Based Therapeuticcs for Chikungunya Virus
    Michael S Diamond; Fiscal Year: 2013
    ..Overall, the generation of strongly protective humanized MAbs against CHIKV will foster the clinical development of immunotherapeutic agents against CHIKV infection. ..
  4. Science in a Culture of Mentoring
    Judith A James; Fiscal Year: 2011
    ..Core expands on OMRF's strengths in human autoantibody detection, cytokine/chemokine measurements, epitope mapping and serum analyte development...
  5. Passive Immunotherapy for Pediatric Mutans Streptococcal Infections
    Martin Taubman; Fiscal Year: 2009
    ..antibody fragment (scFv) phage display library, subsequent bivalent scFvFc antibody (minibody) construction, epitope mapping and affinity determination to maximize inhibition potential, followed by full human monoclonal antibody (HMR) ..
  6. Science in a Culture of Mentoring
    Judith A James; Fiscal Year: 2013
    ..Core expands on OMRF's strengths in human autoantibody detection, cytokine/chemokine measurements, epitope mapping and serum analyte development...
  7. Custom Oligonucleotide Libraries synthesis
    Jean Marie Rouillard; Fiscal Year: 2010
    ..are used as a research tools in many applications such as studies on gene silencing, protein-DNA interaction, epitope mapping or even antimicrobial peptides. There are no limits for applications than the imagination of scientists...
  8. Systems Biological Analysis of Innate and Adaptive Responses to Vaccination
    Bali Pulendran; Fiscal Year: 2013
    ..These will be supported by an Adminstrative Core (Pulendran), a Clinical Core (Mulligan), an Epitope Mapping Core (Sette), and a Computational Core (Haining)...
  9. Production of Cross-Neutralizing HIV-1 Antibodies from Single B Cells
    Miroslaw K Gorny; Fiscal Year: 2011
    ..mAbs combining two or three inhibitory functions (neutralization, ADCC and/or ADCVI) will have priority for epitope mapping followed by those mAbs that cross-neutralize only and non-neutralizing mAbs with the FcγR-mediated ..
  10. HIV Variation in Injection Drug Users: Mapping Broadly Neutralizing Antibodies
    PHILLIP WAYNE BERMAN; Fiscal Year: 2013
    ..Our new method of epitope mapping makes use of the swarm of closely related virus variants that occur within each HIV-infected individual as a ..
  11. Low cost, high density custom peptide microarray synthesis205
    Jean Marie Rouillard; Fiscal Year: 2010
    ..emerged as a powerful tool for mapping receptor-ligand interactions in drug discovery applications as well as epitope mapping. There is a huge, but largely unrealized, potential for peptide microarray applications in drug discovery, ..
  12. Computational Approaches to Studying Immunodominance.
    Tomer Hertz; Fiscal Year: 2013
    ..2003). Recent work has shown the viability of using such prediction tools in epitope mapping studies (Moutaftsi et al., 2006;Assarsson et al., 2008)...
  13. Molecular Determinants of Cross-Reactive Antibody Response to Influenza in Humans
    James E Crowe; Fiscal Year: 2013
    ..By epitope mapping using biochemical arid biologic studies, coupled with studies to determine co-crystal stuctures, we will ..
  14. Broadly Neutralizing Monoclonal Antibodies Against HIV-1
    George K Lewis; Fiscal Year: 2013
    ..profiles of anti-Env responses will be determined by limiting dilution analysis, mAb isolation, and epitope mapping to identify broadly neutralizing mAbs...
  15. Broad Neutralizing Monoclonal Antibodies From HIV Controllers
    George K Lewis; Fiscal Year: 2010
    ..profiles of anti-Env responses will be determined by limiting dilution analysis, mAb isolation, and epitope mapping to determine the relative dominance of BMem clones specific for different Env-epitopes...
  16. Deep Sequencing to Screen Functional Antibody Epitopes
    TIMOTHY ANDREW WHITEHEAD; Fiscal Year: 2013
    ..The project involves two aims. The first aim is to develop a novel epitope mapping strategy that couples yeast display with deep sequencing to generate unprecedented sequence-function maps of ..
  17. Dengue Epitope Vaccine,Tetravalent & MHCII-Targeted
    J Thomas August; Fiscal Year: 2007
    ..b>Epitope mapping technologies will be applied with both mouse and human antisera to identify the minimum sequences of the ..
  18. CMV-Specific CD8+ T Cell Responses in Kidney Transplant Recipients
    LAURA L GIBSON; Fiscal Year: 2010
    ..experiments will examine CMV protein-specific CD8+ T cell responses against several gene products, and fine epitope mapping studies will allow the synthesis of MHC class l-peptide tetramer complexes to identify CMV epitope-specific ..
  19. High-throughput Epitope Mapping of Anti-Viral Antibodies
    BENJAMIN JACOB DORANZ; Fiscal Year: 2011
    ....
  20. BIOMEDICAL (BASIC)
    KEMMONS TUBBS; Fiscal Year: 2010
    ..The project will develop reproducible methods to demonstrate epitope mapping for monoclonal antibodies of interest to NCI tested by multiple sampling and quantifiable results...
  21. BIOMEDICAL (BASIC)
    KEMMONS TUBBS; Fiscal Year: 2009
    ..The project will develop reproducible methods to demonstrate epitope mapping for monoclonal antibodies of interest to NCI tested by multiple sampling and quantifiable results...
  22. BIOMEDICAL (BASIC)
    KEMMONS TUBBS; Fiscal Year: 2011
    ..The project will develop reproducible methods to demonstrate epitope mapping for monoclonal antibodies of interest to NCI tested by multiple sampling and quantifiable results...
  23. Fine epitope mapping of B cell response in patients with hemophilia A: Pilot
    Shannon L Meeks; Fiscal Year: 2013
    ..In a subset of patients we will have two samples from different points in time and we will compare the change of epitope spectrum and response to fVIII at the different time points. ..
  24. ANTIGENIC STRUCTURE OF PURIFIED HOUSE DUST MITE ALLERGEN
    Martin Chapman; Fiscal Year: 1992
    ..b>Epitope mapping studies will be carried out by comparing antibody binding to denatured allergens, chemically modified ..
  25. CHARACTERIZATION OF THE PEMPHIGUS FOLIACEUS ANTIGEN
    Grant Anhalt; Fiscal Year: 1993
    ..Monoclonal antibodies will be used for epitope mapping by competitive studies with the PF AAb...
  26. STRUCTURAL BIOLOGY OF THE ALLERGIC RESPONSE
    STEVEN ALMO; Fiscal Year: 2002
    ..The structural information, when combined with epitope mapping, will provide the sequences, positions and detailed conformations of the epitopes responsible for IgE binding ..
  27. ROLE OF ANTI-PROTEINASE-3 IN WEGENER'S VASCULOPATHY
    Judith James; Fiscal Year: 2003
    ..These previous studies provide the technical background for this proposal. Epitope mapping experiments of the spliceosomal autoantigens have led to our peptide induced model of lupus autoimmunity (8,9)..
  28. Monitoring the development of anti-Env Abs during HIV infection
    LEONIDAS A STAMATATOS; Fiscal Year: 2013
    ..Such studies are significant since they will provide new information on the mechanisms of HIV- mediated pathogenesis that leads to AIDS. ..
  29. SIGNAL TRANSDUCTION OF A G PROTEIN COUPLED RECEPTOR
    Athan Kuliopulos; Fiscal Year: 1999
    ..Localization of the site of labeling will be done by epitope mapping, electrospray mass spectrometry, and sequencing of proteolytic fragments...
  30. EXPERIMENTAL AUTOIMMUNE GRAVES' DISEASE
    Bellur Prabhakar; Fiscal Year: 2003
    ..To define TSHR epitopes to which pathogenic antibodies bind, we will carryout epitope mapping studies...
  31. Jo-1-specific T Cell Responses in Polymyositis
    DANA ASCHERMAN; Fiscal Year: 2007
    ..TCR sequencing as well as epitope mapping studies using both Jo-1 fragments and linear peptides will then permit further characterization of the Jo-1-..
  32. STRUCTURAL ANALYSIS OF THROMBIN SPECIFICITY SITS
    HENRY JAKUBOWSKI; Fiscal Year: 1991
    ..The epitope mapping will also be extended to the production of cloned Fab fragments recognizing protein C, and thrombomodulin, to ..
  33. NUCLEAR PROTEINS AND AUTOIMMUNITY
    SALLIE HOCH; Fiscal Year: 1992
    ..Detailed antigen analysis will be facilitated by: epitope mapping; interspecies comparisons at both the peptide and genomic (Southern blot analysis) levels; exploration of ..
  34. INTERACTION OF GPIIB-IIIA WITH THE PLATELET CYTOSKELETON
    Stephen Lam; Fiscal Year: 2003
    ..By chemical cross-linking studies, epitope mapping of inhibitory antibodies, and site directed mutagenesis, we will determine the critical residues in talin ..
  35. Hypocretin System Immune Diathesis in Human Narcolepsy
    John Black; Fiscal Year: 2004
    ..Results will be correlated between CSF and serum and epitope mapping will be done using positive sera...
  36. GLYCOPROTEINS IIB AND IIIA IN PLATELETS AND NEUTROPHILS
    Lisa Jennings; Fiscal Year: 1991
    ..Monoclonal antibodies prepared by this laboratory will be used as specialized probes for epitope mapping. Two techniques will be used primarily: 1) the western blot technique with purified intact glycoproteins and ..
  37. AFP-BASED PRIME-BOOST IMMUNOTHERAPY OF HCC
    LISA BUTTERFIELD; Fiscal Year: 2006
    ..We have performed extensive CD8 T cell epitope mapping and identified several immunodominant and subdominant AFP-derived peptides...
  38. A MODEL SYSTEM FOR STUDY OF CELL-MATRIX INTERACTION
    Kurt Gehlsen; Fiscal Year: 1993
    ..laminin, fibronectin and alpha3beta1 in solid phase binding assays, affinity chromatography, crosslinking epitope mapping experiments and for monoclonal antibody production...
  39. AUTOIMMUNE ANTIGENS AND SYSTEMIC SCLEROSIS
    SALLIE HOCH; Fiscal Year: 1993
    ..Delineation of properties essential to the centromere polypeptides as antigens will include: detailed epitope mapping; analysis of shared epitopes among the various antigens; production of monoclonal and polyclonal antibodies ..
  40. AUTOANTIGENS AND RHEUMATIC DISEASE
    LUIS ROKEACH; Fiscal Year: 1992
    ..of functional domains by sequence comparison as well as relatedness to other autoantigens and proteins; epitope mapping by rDNA techniques; generation of monospecific antibodies to the individual proteins/epitopes by using rDNA ..
  41. GENETICS AND FUNCTION OF MENINGOCOCCAL SURFACE PROTEINS
    JANNE CANNON; Fiscal Year: 1993
    ..Information from the epitope mapping will be used in an analysis of a possible recombinational mechanism that could contribute to structural and ..
  42. IMMUNOCHEMISTRY OF FACTOR VIII INHIBITORS
    CAROL FULCHER; Fiscal Year: 1992
    ..Further studies proposed in this application include: epitope mapping of FVIII inhibitors using proteolytic or recombinant DNA produced fragments of FVIII and antibodies of ..
  43. ANTIBODIES AGAINST PHOSPHORYLATED 5HT2C RECEPTORS
    JON BACKSTROM; Fiscal Year: 2000
    ..Specific aims are designed to: 1) identify phosphorylated sites of 5-HT2C receptors. Phospho-epitope mapping experiments will be performed with 5-HT2C receptor antibodies to identify potential phosphorylated regions of ..
  44. Novel Vaccine Targeting the Sugar Coat of HIV-1
    Lai Xi Wang; Fiscal Year: 2004
    ..b>Epitope mapping demonstrated that a high-mannose oligosaccharide cluster most likely constitutes the actual epitopes of the ..
  45. BIOMEDICAL RESEARCH SUPPORT
    Marie Cowan; Fiscal Year: 1992
    ..Finally, epitope mapping analysis of the HIV gp120/gp41 by the development of monoclonal reagents has led to the identification of ..
  46. Genetic Immunotherapy for Hepatocellular Cancer
    James Economou; Fiscal Year: 2004
    ..We have performed extensive epitope mapping of hAFP and have identified 4 immunodominant and as many as 10 subdominant peptide epitopes in the context of ..
  47. ENDOCAM/PECAM--A NOVEL VASCULAR CELL ADHESION MOLECULE
    Steven Albelda; Fiscal Year: 1999
    ..antibodies, evaluation of PECAM-1 constructs containing specific point mutations in key regions identified by epitope mapping, and by testing the ability of peptides that mimic the putative functional regions of the extracellular ..
  48. Multivalent gp41 Peptides as Novel Immunogens
    Lai Xi Wang; Fiscal Year: 2004
    ..Recent studies on the mechanism of viral membrane fusion, as well as the epitope mapping of three broadly neutralizing anti-gp41 antibodies 2F5, 4E10, and Z13 suggest that the C terminal ectodomain ..
  49. Fine specificity of scleroderma autoantibodies
    Judith James; Fiscal Year: 2005
    ..These previous studies provide the technical background for this proposal. Epitope mapping experiments of the lupus spliceosomal autoantigens have led to a peptide induced model of lupus autoimmunity...
  50. BIOCHEMICALLY DETERMINED TOPOLOGY OF AN RENAL K+ CHANNEL
    RUTH SCHWALBE; Fiscal Year: 2002
    ..The experimental design is to map the extracellular segments by N-glycosylaton substitution mutagenesis and epitope mapping, and to verify the predicted intracellular segments by protease digestion for ROMK1, as well as IRK1...
  51. SURFACE PROTEINS OF NEISSERIA GONORRHOEAE
    Milan Blake; Fiscal Year: 1991
    ..Several epitopes available on monomers become concealed during this assembly. Through epitope mapping with monoclonal antibodies to the monomer, these epitopes will be defined...
  52. APOPTOSIS-MODIFIED SELF ANTIGEN IN RHEUMATIC DISEASE
    Eric Greidinger; Fiscal Year: 2002
    ..b>Epitope mapping will be performed with all apoptotic Ul-70kDa specific antisera and Fab to identify the immunologically ..
  53. DIET EFFECTS--LIPOPROTEIN METABOLISM & PLATELET FUNCTION
    Henry Ginsberg; Fiscal Year: 1990
    ..This parallel design approach will enable us to carry out both clinical trials and perform sophisticated laboratory measurements...
  54. INHIBITORS OF RETROVIRAL PROTEASE
    GARLAND MARSHALL; Fiscal Year: 1993
    ..The affinities of the observed inhibitors will be optimized by a variation of the Geysen procedure for epitope mapping HIV-l function DNA clones will be constructed with deletions or mutations in the protease region to determine ..
  55. Macaque MHC Class II Tetramers Specific for HIV and SIV
    Marcelo Kuroda; Fiscal Year: 2004
    ..Specifically, we will: 1. Perform CD4+ T lymphocyte epitope mapping in SHIV- and SIV-infected rhesus monkeys. 2...
  56. Impact of CD8+ T cell functionality on HIV viral escape
    MICHAEL BETTS; Fiscal Year: 2006
    ..Our findings will further our understanding of HIV disease, and assist in the development of an HIV vaccine. ..
  57. EPITOPE MAPPING OF HIV-1 ENHANCING ANTIBODIES
    A Robert Neurath; Fiscal Year: 1993
    ....
  58. ADVANCED DIAGNOSTICS USING RNA/PROTEIN FUSION TECHNOLOGY
    Brent Kreider; Fiscal Year: 2002
    ..were selected and will be further evaluated for performance characteristics such as affinity, specificity, epitope mapping, and ability to function on a solid phase surface...
  59. HIV-1 Vaccine Based on Chimp Serotypes of Adenovirus
    Hildegund Ertl; Fiscal Year: 2006
    ..by site-directed mutagenesis using structural information from X-ray crystallography and modeling as well as epitope mapping of virus neutralizing antibodies...
  60. ANTI-CD4 ANTIBODY AS THERAPEUTIC/PREVENTIVE HIV VACCINE
    Chang Yuil Kang; Fiscal Year: 1990
    ..murine anti-CD4 MAbs and characterize them in vitro to identify candidates for gp120-mimicking antibodies by epitope mapping and in vitro virus neutralization assays...
  61. AFFINITY IMAGE-INSTRUMENTATION FOR DRUG DISCOVERY
    Srivatsa Venkatasubbarao; Fiscal Year: 2000
    ..During Phase I of this project two model systems will be tested: (l) epitope mapping of anti-b-endorphin antibody and (2) probing peptide and nonpeptide ligands that bind to the biotin binding ..
  62. LEUKOCYTE DEPENDENT REGULATION OF COAGULATION
    Dario Altieri; Fiscal Year: 1999
    ..The long term objective of these studies is to identify highly selective antagonists of leukocyte adhesion reactions that will reduce vascular damage without affecting cellular hemostatic functions. ..