restriction mapping

Summary

Summary: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.

Top Publications

  1. pmc Linkage mapping and comparative genomics using next-generation RAD sequencing of a non-model organism
    Simon W Baxter
    Department of Zoology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 6:e19315. 2011
  2. pmc Rapid SNP discovery and genetic mapping using sequenced RAD markers
    Nathan A Baird
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, United States of America
    PLoS ONE 3:e3376. 2008
  3. pmc Local de novo assembly of RAD paired-end contigs using short sequencing reads
    Paul D Etter
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, United States of America
    PLoS ONE 6:e18561. 2011
  4. ncbi Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes
    S L Mansour
    Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City 84112
    Nature 336:348-52. 1988
  5. pmc Genome type analysis of adenovirus types 3 and 7 isolated during successive outbreaks of lower respiratory tract infections in children
    Yae Jean Kim
    Department of Pediatrics, College of Medicine, Seoul National University, Seoul, Korea
    J Clin Microbiol 41:4594-9. 2003
  6. ncbi Measurement of telomere length by the Southern blot analysis of terminal restriction fragment lengths
    Masayuki Kimura
    The Center for Human Development and Aging, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
    Nat Protoc 5:1596-607. 2010
  7. ncbi MiCA: a web-based tool for the analysis of microbial communities based on terminal-restriction fragment length polymorphisms of 16S and 18S rRNA genes
    Conrad Shyu
    Department of Computer Science, University of Idaho, Moscow, ID 83844, USA
    Microb Ecol 53:562-70. 2007
  8. ncbi Expression of TTK, a novel human protein kinase, is associated with cell proliferation
    G B Mills
    Oncology Research, Toronto General Hospital, Ontario, Canada
    J Biol Chem 267:16000-6. 1992
  9. ncbi Study of the authenticity of commercial wine yeast strains by molecular techniques
    M T Fernández-Espinar
    Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos CSIC, Burjassot, Valencia, Spain
    Int J Food Microbiol 70:1-10. 2001
  10. pmc Molecular analysis of Mycobacterium kansasii isolates from the United States
    Yansheng Zhang
    Department of Microbiology, The University of Texas Health Center, Tyler, Texas 75708, USA
    J Clin Microbiol 42:119-25. 2004

Research Grants

  1. Nanofluidic Platforms for High Resolution Mapping of Genomic DNA
    JOHN MICHAEL RAMSEY; Fiscal Year: 2013
  2. STRUCTURE AND GENETIC CONTROL OF IGA
    Katherine Knight; Fiscal Year: 2000
  3. HEX EXPRESSION AND REGULATION IN DEVELOPING LUNG
    CLIFFORD BOGUE; Fiscal Year: 2000
  4. GENETIC ANALYSIS OF CHARCOT-MARIE-TOOTH DISEASE
    PHILLIP CHANCE; Fiscal Year: 1992
  5. ANOXIA RESPONSIVE VL30 ELEMENTS
    Garth Anderson; Fiscal Year: 1992
  6. MECHANISMS & REGULATION OF PROTEIN SYNTHESIS
    ALBERT WAHBA; Fiscal Year: 1992
  7. AMPLIFICATION--MODEL FOR GENETIC INSTABILITY IN CANCER
    JOYCE HAMLIN; Fiscal Year: 2005
  8. MUTAGENIC AND RECOMBINOGENIC EFFECTS OF GAMMA RAYS
    William Taylor; Fiscal Year: 1990
  9. IN VIVO EXPRESSION OF SCHWANN CELL NA CHANNEL
    GING WANG; Fiscal Year: 1990
  10. CLONING OF THE INVASIVENESS GENE FROM BARTONELLA BACILLI
    ETHELEEN MC GINNIS; Fiscal Year: 1993

Detail Information

Publications239 found, 100 shown here

  1. pmc Linkage mapping and comparative genomics using next-generation RAD sequencing of a non-model organism
    Simon W Baxter
    Department of Zoology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 6:e19315. 2011
    ..mori chromosomes shows for the first time, genetic synteny appears common beyond the Macrolepidoptera. RAD sequencing is a powerful system capable of rapidly generating chromosome specific data for non-model organisms...
  2. pmc Rapid SNP discovery and genetic mapping using sequenced RAD markers
    Nathan A Baird
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, United States of America
    PLoS ONE 3:e3376. 2008
    ..Sequencing of RAD markers is an integrated platform for SNP discovery and genotyping. This approach should be widely applicable to genetic mapping in a variety of organisms...
  3. pmc Local de novo assembly of RAD paired-end contigs using short sequencing reads
    Paul D Etter
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, United States of America
    PLoS ONE 6:e18561. 2011
    ....
  4. ncbi Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes
    S L Mansour
    Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City 84112
    Nature 336:348-52. 1988
    ..The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene...
  5. pmc Genome type analysis of adenovirus types 3 and 7 isolated during successive outbreaks of lower respiratory tract infections in children
    Yae Jean Kim
    Department of Pediatrics, College of Medicine, Seoul National University, Seoul, Korea
    J Clin Microbiol 41:4594-9. 2003
    ..In conclusion, the data confirm that Ad3 genome types are more diverse than those of Ad7 and suggest that shifts of genome types may occur during successive outbreaks of Ad3 and Ad7...
  6. ncbi Measurement of telomere length by the Southern blot analysis of terminal restriction fragment lengths
    Masayuki Kimura
    The Center for Human Development and Aging, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
    Nat Protoc 5:1596-607. 2010
    ..A skilled technician can measure TRF length in ∼130 samples per week...
  7. ncbi MiCA: a web-based tool for the analysis of microbial communities based on terminal-restriction fragment length polymorphisms of 16S and 18S rRNA genes
    Conrad Shyu
    Department of Computer Science, University of Idaho, Moscow, ID 83844, USA
    Microb Ecol 53:562-70. 2007
    ..APLAUS estimates the relative abundances of populations and reports a listing of phylotypes that are consistent with the empirical data. MiCA is accessible at http://mica.ibest.uidaho.edu/...
  8. ncbi Expression of TTK, a novel human protein kinase, is associated with cell proliferation
    G B Mills
    Oncology Research, Toronto General Hospital, Ontario, Canada
    J Biol Chem 267:16000-6. 1992
    ..Taken together, these findings suggest that expression of TTK, a previously unidentified member of the family of kinases which can phosphorylate serine, threonine, and tyrosine hydroxyamino acids, is associated with cell proliferation...
  9. ncbi Study of the authenticity of commercial wine yeast strains by molecular techniques
    M T Fernández-Espinar
    Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos CSIC, Burjassot, Valencia, Spain
    Int J Food Microbiol 70:1-10. 2001
    ..Additionally, commercial starters of Saccharomyces uvarum and Saccharomyces bayanus have been re-identified as Saccharomyces cerevisiae...
  10. pmc Molecular analysis of Mycobacterium kansasii isolates from the United States
    Yansheng Zhang
    Department of Microbiology, The University of Texas Health Center, Tyler, Texas 75708, USA
    J Clin Microbiol 42:119-25. 2004
    ..The fact that a single clone of M. kansasii is responsible for most cases of human disease suggests that specific virulence factors may be associated with this specific genotype...
  11. pmc Complete nucleotide sequence of ubiquitous plasmid pEA29 from Erwinia amylovora strain Ea88: gene organization and intraspecies variation
    G C McGhee
    Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824 1312, USA
    Appl Environ Microbiol 66:4897-907. 2000
    ..Plasmid-cured strains exhibited reduced virulence and modified colony morphology on minimal medium without thiamine, indicating that some of the genes in pEA29 play a role in the physiology or metabolism of E. amylovora...
  12. ncbi 'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma pyri' and 'Candidatus Phytoplasma prunorum', the causal agents of apple proliferation, pear decline and European stone fruit yellows, respectively
    Erich Seemüller
    Biologische Bundesanstalt für Land und Forstwirtschaft, Institut für Pflanzenschutz im Obstbau, D 69221 Dossenheim, Germany
    Int J Syst Evol Microbiol 54:1217-26. 2004
    ..Until more information on this phytoplasma is available, it is proposed that the PYLR phytoplasma should be regarded as a subtype of 'Candidatus Phytoplasma pyri'...
  13. ncbi A new class of tissue-specifically methylated regions involving entire CpG islands in the mouse
    Masako Suzuki
    Laboratory of Cellular Biochemistry, Veterinary Medical Sciences Animal Resource Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    Genes Cells 12:1305-14. 2007
    ..T-DMRs at CpG islands are functionally important gene regulatory elements that may now be categorized into two classes: T-DMRs involving a subregion of the CpG island and those that occupy the whole CpG island...
  14. pmc Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S-23S rRNA gene spacer and restriction endonucleases
    A Roth
    Institut für Mikrobiologie und Immunologie, Lungenklinik Heckeshorn, 14109 Berlin, Germany
    J Clin Microbiol 38:1094-104. 2000
    ....
  15. pmc Identification of a GTPase-activating protein homolog in Schizosaccharomyces pombe
    Y Imai
    Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan
    Mol Cell Biol 11:3088-94. 1991
    ....
  16. ncbi The pregnancy-specific glycoprotein (PSG) gene cluster on human chromosome 19: fine structure of the 11 PSG genes and identification of 6 new genes forming a third subgroup within the carcinoembryonic antigen (CEA) family
    S Teglund
    Department of Immunology, University of Umea, Sweden
    Genomics 23:669-84. 1994
    ..Unlike any of the known CEA family genes, however, they seem to lack the exon for an IgV-like N-terminal domain...
  17. ncbi Quantitative analysis of chromosome conformation capture assays (3C-qPCR)
    Hélène Hagège
    UMR5535 CNRS UMII, IFR122, Institut de Genetique Moleculaire de Montpellier, IGMM, 1919, route de Mende, 34293 Montpellier Cedex 5, France
    Nat Protoc 2:1722-33. 2007
    ..Here, we provide a detailed protocol for this method, which we have named 3C-qPCR. Once preliminary controls and optimizations have been performed, the whole procedure (3C assays and quantitative analyses) can be completed in 7-9 days...
  18. ncbi Male mice defective in the DNA mismatch repair gene PMS2 exhibit abnormal chromosome synapsis in meiosis
    S M Baker
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201 3098, USA
    Cell 82:309-19. 1995
    ..These observations suggest links among mismatch repair, genetic recombination, and chromosome synapsis in meiosis...
  19. pmc Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe
    P J Miller
    Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405
    Mol Cell Biol 14:1075-83. 1994
    ..However, the cdc42 mutants did exhibit an abnormal morphological phenotype of large, misshapen cells, suggesting that S. pombe Cdc42p is involved in controlling polarized cell growth...
  20. ncbi Epigenetic marks by DNA methylation specific to stem, germ and somatic cells in mice
    Kunio Shiota
    Laboratory of Cellular Biochemistry, Animal Resource Sciences Veterinary Medical Sciences, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Genes Cells 7:961-9. 2002
    ..We recently found that some CpG islands had a tissue-dependent and differentially methylated region (T-DMR) in normal tissues, raising the possibility that there may be more CpG islands capable of differential methylation...
  21. ncbi Shotgun optical mapping of the entire Leishmania major Friedlin genome
    Shiguo Zhou
    Laboratory for Molecular and Computational Genomics, UW Biotechnology Center, University of Wisconsin Madison, 425 Henry Mall, Madison, WI 53706, USA
    Mol Biochem Parasitol 138:97-106. 2004
    ..Our results also showed the potential use of optical maps as a means to detect and characterize map segmental duplication within genomes...
  22. pmc Whole-genome shotgun optical mapping of Rhodospirillum rubrum
    Susan Reslewic
    Laboratory for Molecular and Computational Genomics, University of Wisconsin Madison, UW Biotechnology Center, 425 Henry Mall, Madison, WI 53706, USA
    Appl Environ Microbiol 71:5511-22. 2005
    ..With a resolution ranging from 6.5 kb (previously published) to 45 kb (reported here), optical mapping advances a "molecular cytogenetics" approach to solving problems in genomic analysis...
  23. ncbi A kinesin-like protein required for distributive chromosome segregation in Drosophila
    P Zhang
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461
    Cell 62:1053-62. 1990
    ..Thus, the nod protein is a member of the kinesin superfamily and may be a microtubule motor...
  24. pmc Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily
    C Dascher
    Department of Molecular Genetics, Max Planck Institute for Biophysical Chemistry, Gottingen, Federal Republic of Germany
    Mol Cell Biol 11:872-85. 1991
    ..SLY12 but not SLY41 is an essential gene. The SLY2 null mutant is cold and heat sensitive. The SLY gene products may comprise elements of the protein transport machinery...
  25. ncbi Genomic and genetic definition of a functional human centromere
    M G Schueler
    Department of Genetics, Case Western Reserve University School of Medicine and Center for Human Genetics, and, Research Institute, University Hospitals of Cleveland, Cleveland, OH 44106, USA
    Science 294:109-15. 2001
    ....
  26. pmc NDC1: a nuclear periphery component required for yeast spindle pole body duplication
    M Winey
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder 80309 0347
    J Cell Biol 122:743-51. 1993
    ..These findings suggest that the NDC1 protein acts within the nuclear envelope to mediate insertion of the nascent SPB...
  27. ncbi Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean
    Sylvia Cardoso Leao
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo Escola Paulista de Medicina, Rua Botucatu, 862 3 degrees andar, São Paulo 04023 062, Brazil
    J Microbiol Methods 61:193-9. 2005
    ..In others, improvement in critical points is still necessary...
  28. ncbi In silico analysis of complete bacterial genomes: PCR, AFLP-PCR and endonuclease restriction
    Joseba Bikandi
    Department of Immunology, Microbiology and Parasitology, University of the Basque Country, Paseo de la Universidad, 7, 01006 Vitoria Gasteiz, Spain
    Bioinformatics 20:798-9. 2004
    ..Other tools that have been included in this site are ORF search by name and DNA to protein translation as well as restriction digestion of user-defined DNA sequences...
  29. pmc Restriction mapping in nanofluidic devices
    Robert Riehn
    Departments of Physics, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 102:10012-6. 2005
    We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm...
  30. ncbi Analysis and quantification of multiple methylation variable positions in CpG islands by Pyrosequencing
    Jorg Tost
    Centre National de Genotypage, Evry, France
    Biotechniques 35:152-6. 2003
  31. ncbi Restriction mapping of βS locus among Tunisian sickle-cell patients
    Moumni Imen
    Laboratory of molecular and cellular hematology, Pasteur Institute of Tunis, University of Tunis Elmanar, Tunisia
    Am J Hum Biol 23:815-9. 2011
    ..In our study, the haplotypes linked to the Tunisian β(S) mutation are determined to improve our knowledge of the chromosomal background of the β-globin gene in sickle-cell anemia in Tunisia...
  32. pmc Human immunodeficiency virus type 1 recombination: rate, fidelity, and putative hot spots
    Jianling Zhuang
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Virol 76:11273-82. 2002
    ....
  33. pmc An outbreak of keratitis caused by Mycobacterium immunogenum
    Jorge Luiz Mello Sampaio
    Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo Escola Paulista de Medicina, Rua Botucatu, 862 3 degrees andar, 04023 062 Sao Paulo, Brazil
    J Clin Microbiol 44:3201-7. 2006
    ..This is the first report of an outbreak where this species was isolated from infected tissues...
  34. ncbi Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1
    Katsuyuki Tanaka
    Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1 3 1, Higashi Hiroshima 739 8526, Japan
    Genes Genet Syst 81:373-80. 2006
    ..This datum suggests that the line of the strain is a major one in biovar 3 in Japan. Evolution of the genome of the strain is discussed based on the data...
  35. ncbi hsp65 PCR-restriction enzyme analysis (PRA) for identification of mycobacteria in the clinical laboratory
    C F da Silva
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, , , SP, 04023-062, Brasil
    Rev Inst Med Trop Sao Paulo 43:25-8. 2001
    ..The results of this work showed that PRA could improve identification of mycobacteria in a routine setting because it is accurate, fast, and cheaper than conventional phenotypic identification...
  36. ncbi Aberrant methylation frequency of TNFRSF10C promoter in pancreatic cancer cell lines
    Hui hua Cai
    Department of General Surgery, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
    Hepatobiliary Pancreat Dis Int 10:95-100. 2011
    ....
  37. pmc Chromosome map of Xanthomonas campestris pv. campestris 17 with locations of genes involved in xanthan gum synthesis and yellow pigmentation
    Y H Tseng
    Institute of Molecular Biology and Department of Botany, National Chung Hsing University, Taichung 402, Taiwan
    J Bacteriol 181:117-25. 1999
    ..This is the first map for X. campestris and would be useful for genetic studies of this and related Xanthomonas species...
  38. pmc A cytoskeleton-related gene, uso1, is required for intracellular protein transport in Saccharomyces cerevisiae
    H Nakajima
    Department of Agricultural Chemistry, University of Tokyo, Japan
    J Cell Biol 113:245-60. 1991
    ..These observations suggest that Uso1 protein plays a role as a cytoskeletal component in the protein transport from the ER to the later secretory compartments...
  39. pmc The active spatial organization of the beta-globin locus requires the transcription factor EKLF
    Roy Drissen
    Erasmus University Medical Center, Department of Cell Biology, The Netherlands
    Genes Dev 18:2485-90. 2004
    ..We conclude that transcription factors can play an essential role in the three-dimensional organization of gene loci...
  40. ncbi slow border cells, a locus required for a developmentally regulated cell migration during oogenesis, encodes Drosophila C/EBP
    D J Montell
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210
    Cell 71:51-62. 1992
    ..Drosophila C/EBP may be required for the expression of gene products mediating border cell migration...
  41. ncbi twine, a cdc25 homolog that functions in the male and female germline of Drosophila
    L Alphey
    Department of Biochemistry, The University, Dundee, Scotland
    Cell 69:977-88. 1992
    ..Meiosis does not occur in homozygous twine males, which produce cysts containing 16 rather than 64 spermatids...
  42. pmc Single-molecule approach to bacterial genomic comparisons via optical mapping
    Shiguo Zhou
    Laboratory for Molecular and Computation Genomics, University of Wisconsin Madison, Madison, WI 53706, USA
    J Bacteriol 186:7773-82. 2004
    ..Our results suggest that genomic rearrangements and chromosomal breakpoints are readily identified and annotated against a prototypic sequenced strain by using the tools of optical mapping...
  43. ncbi The implications of heterogeneous DNA methylation for the accurate quantification of methylation
    Thomas Mikeska
    Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, Locked Bag 1, A Beckett Street, Melbourne, Victoria 8006, Australia
    Epigenomics 2:561-73. 2010
    ..The adoption of digital approaches will enable the effective use of heterogeneous DNA methylation as a cancer biomarker...
  44. ncbi The C. elegans unc-104 gene encodes a putative kinesin heavy chain-like protein
    A J Otsuka
    Department of Biological Sciences, Illinois State University, Normal 61761 6901
    Neuron 6:113-22. 1991
    ..Although the primary sequence relatedness to kinesin is weak in the remainder of the molecule, the predicted secondary structure and regional isoelectric points are similar to kinesin heavy chain...
  45. pmc BET3 encodes a novel hydrophilic protein that acts in conjunction with yeast SNAREs
    G Rossi
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Mol Biol Cell 6:1769-80. 1995
    ..These findings support the hypothesis that Bet3p may act before the assembly of the SNARE complex...
  46. ncbi Chromosomal assignment of human genomic NotI restriction fragments in a two-dimensional electrophoresis profile
    H Yoshikawa
    Institute for Molecular and Cellular Biology, Osaka University, Japan
    Genomics 31:28-35. 1996
    ..We report here the first-generation CA-RLGS map of the human genome...
  47. pmc GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway
    J Albertyn
    Department of Microbiology and Biochemistry, Faculty of Science, University of the Orange Free State, Bloemfontein, South Africa
    Mol Cell Biol 14:4135-44. 1994
    ..Thus, the HOG pathway most probably has additional targets in the mechanism of adaptation to hypertonic medium...
  48. ncbi The human pregnancy-specific glycoprotein genes are tightly linked on the long arm of chromosome 19 and are coordinately expressed
    J Thompson
    Institute of Immunobiology, University of Freiburg, FRG
    Biochem Biophys Res Commun 167:848-59. 1990
    ..Due to the close proximity of these genes and their coordinated expression pattern, common transcriptional regulatory elements may exist...
  49. pmc Altered hepatic transport of immunoglobulin A in mice lacking the J chain
    B A Hendrickson
    Division of Infectious Diseases, Children s Hospital, Boston, Massachusetts, USA
    J Exp Med 182:1905-11. 1995
    ..Furthermore, the J chain is essential for efficient hepatic pIgR transport of IgA...
  50. ncbi Aberrant CpG-island methylation has non-random and tumour-type-specific patterns
    J F Costello
    1 Ludwig Institute for Cancer Research, University of California San Diego, La Jolla, California, USA
    Nat Genet 24:132-8. 2000
    ..The expression of many of these genes was reactivated by experimental demethylation in cultured tumour cells. Thus, the methylation of particular subsets of CpG islands may have consequences for specific tumour types...
  51. ncbi A second B cell-specific enhancer 3' of the immunoglobulin heavy-chain locus
    S Pettersson
    Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
    Nature 344:165-8. 1990
    ..The IgH 3' enhancer could therefore play a part in the activation of the translocated c-myc genes in rat immunocytomas, mouse plasmacytomas and Burkitt lymphomas...
  52. pmc RAD marker microarrays enable rapid mapping of zebrafish mutations
    Michael R Miller
    Institute of Molecular Biology, University of Oregon, 1370 Franklin Blvd, Eugene, Oregon 97403, USA
    Genome Biol 8:R105. 2007
    ..The RAD approach is highly effective for genetic mapping in zebrafish and is an attractive option for mapping in other organisms...
  53. ncbi Sensitive and quantitative universal Pyrosequencing methylation analysis of CpG sites
    S Colella
    University of Texas M D Anderson Cancer Center, Houston, TX, USA
    Biotechniques 35:146-50. 2003
  54. pmc Optical mapping as a routine tool for bacterial genome sequence finishing
    Phil Latreille
    Monsanto Company, 800 North Lindbergh Boulevard St Louis, MO 63167, USA
    BMC Genomics 8:321. 2007
    ..This included a stretch of about 12 Kb that is over 99.9% identical between the plasmid and chromosome of X. nematophila...
  55. ncbi DNA methylation: a profile of methods and applications
    Mario F Fraga
    Spanish National Cancer Research Center CNIO, Madrid
    Biotechniques 33:632, 634, 636-49. 2002
    ..We hope this review will be a valuable tool in selecting the best techniques to address particular questions concerning the cytosine methylation status of genomic DNA...
  56. pmc A yeast mitogen-activated protein kinase homolog (Mpk1p) mediates signalling by protein kinase C
    K S Lee
    Department of Biochemistry, School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205
    Mol Cell Biol 13:3067-75. 1993
    ....
  57. ncbi Structure and mapping of the human beta-defensin HBD-2 gene and its expression at sites of inflammation
    L Liu
    Departments of Medicine and Pathology and the Will Rogers Institute for Pulmonary Research, UCLA School of Medicine, Los Angeles, CA, USA
    Gene 222:237-44. 1998
    ..The HBD-2 gene is the first member of the human defensin family that is locally inducible by inflammation...
  58. ncbi Identification and differentiation of Fasciola hepatica and Fasciola gigantica using a simple PCR-restriction enzyme method
    Mohammad Bagher Rokni
    Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran
    Exp Parasitol 124:209-13. 2010
    ..Our PCR-restriction enzyme profile is a simple, rapid and reliable method for differentiating F. hepatica and F. gigantica, and can be used for diagnostic and epidemiological purposes...
  59. ncbi KAR3, a kinesin-related gene required for yeast nuclear fusion
    P B Meluh
    Department of Biology, Lewis Thomas Laboratory, Princeton University, New Jersey 08544 1014
    Cell 60:1029-41. 1990
    ..The phenotypes of kar3 mutants suggest that the protein mediates microtubule sliding during nuclear fusion and possibly mitosis...
  60. ncbi A comprehensive systematic approach to identification of influenza A virus genotype using RT-PCR and RFLP
    D P Offringa
    The Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, MD 20852, USA
    J Virol Methods 88:15-24. 2000
    ....
  61. pmc Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands
    J G Herman
    Oncology Center, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 93:9821-6. 1996
    ....
  62. pmc Co-localization of centromere activity, proteins and topoisomerase II within a subdomain of the major human X alpha-satellite array
    Jennifer M Spence
    Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
    EMBO J 21:5269-80. 2002
    ....
  63. ncbi New genome type of adenovirus serotype 19 causing nosocomial infections of epidemic keratoconjunctivitis in Japan
    K Tanaka-Yokogui
    Department of Ophthalmology, Yokohama City University School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Med Virol 65:530-3. 2001
    ..Strains from 1992 and 1993 showed restriction patterns similar to those of the worldwide reported variant 19a for all enzymes used. The changes detected in strains from 1997 and 1998 could be the reason for the recent epidemic...
  64. ncbi Alignment of optical maps
    Anton Valouev
    Department of Mathematics, University of Southern California, Los Angeles, 90089 1113, USA
    J Comput Biol 13:442-62. 2006
    ..This allows us to achieve maximal descriminative power for the alignment score. Our scoring method is naturally embedded within a well known DP framework for finding optimal alignments...
  65. ncbi A NotI-EcoRV promoter library for studies of genetic and epigenetic alterations in mouse models of human malignancies
    Li Yu
    Division of Human Cancer Genetics, Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University, Columbus, OH 43210, USA
    Genomics 84:647-60. 2004
    ..Luciferase assays using NotI-EcoRV clones confirmed that the library is enriched for promoter sequences. Thus, this library will support future genetic and epigenetic studies in mouse models...
  66. pmc Whole-genome shotgun optical mapping of Rhodobacter sphaeroides strain 2.4.1 and its use for whole-genome shotgun sequence assembly
    Shiguo Zhou
    Laboratory for Molecular and Computational Genomics, University of Wisconsin Madison, UW Biotechnology Center, Madison, Wisconsin 53706, USA
    Genome Res 13:2142-51. 2003
    ..Our results show that such maps facilitated the closure of sequence gaps by the early detection of nascent sequence contigs during the course of the whole-genome shotgun sequencing process...
  67. pmc Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer
    Julien Thiry
    Virology and Viral Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, B 4000 Liege, Belgium
    BMC Vet Res 3:26. 2007
    ..In this context, it has been decided to investigate the presence of an alphaherpesvirus spreading in the Belgian free-ranging red deer population...
  68. ncbi Evaluation of polymerase chain reaction and restriction enzyme analysis for routine identification of mycobacteria: accuracy, rapidity, and cost analysis
    Therdsak Prammananan
    Department of Microbiology, Faculty of Medicine at Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Southeast Asian J Trop Med Public Health 36:1252-60. 2005
    ..terrae complex). PCR-REA demonstrated not only accurate results but was also less expensive (2.1 US dollars/sample). This method was rapid with a turn-around time of 30 hours compared with 2-4 weeks for the conventional method...
  69. ncbi Diversity among Lactobacillus paracasei phages isolated from a probiotic dairy product plant
    M L Capra
    Instituto de Lactología Industrial, Universidad Nacional del Litoral, Santiago del Estero 2829, Santa Fe 3000, Argentina
    J Appl Microbiol 107:1350-7. 2009
    ..To evaluate the phage diversity in the environment of a dairy industry which manufactures a product fermented with a probiotic strain of Lactobacillus paracasei...
  70. ncbi Entamoeba histolytica: the serine-rich gene polymorphism-based genetic variability of clinical isolates from Georgia
    Sophia Simonishvili
    National Center for Disease Control and Medical Statistics of Georgia, 9 Asatiani Street, Tbilisi 380077, Georgia
    Exp Parasitol 110:313-7. 2005
    ..histolytica, thus preventing an association of a single genotype with hepatic disease, but supporting the previous finding of extensive genetic diversity among E. histolytica isolates from the same geographic origin...
  71. ncbi Fas-mediated apoptosis and activation-induced T-cell proliferation are defective in mice lacking FADD/Mort1
    J Zhang
    Department of Molecular and Cell Biology, University of California at Berkeley, 94720 3200, USA
    Nature 392:296-300. 1998
    ..These results and the similarities between FADD-/- mice and mice lacking the beta-subunit of the IL-2 receptor suggest that there is an unexpected connection between cell proliferation and apoptosis...
  72. ncbi moz regulates Hox expression and pharyngeal segmental identity in zebrafish
    Craig T Miller
    Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403, USA
    Development 131:2443-61. 2004
    ....
  73. pmc KLP38B: a mitotic kinesin-related protein that binds PP1
    L Alphey
    School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
    J Cell Biol 138:395-409. 1997
    ..This is the first time that a kinesin-related protein has been shown to have such a role. Incomplete lethality of a strong KLP38B allele suggests partial redundancy with one or more additional kinesin-related proteins...
  74. ncbi The gun4 gene is essential for cyanobacterial porphyrin metabolism
    Annegret Wilde
    Institut fur Biologie, Biochemie der Pflanzen, Humboldt Universitat zu Berlin, Chausseestrasse 117, 10115 Berlin, Germany
    FEBS Lett 571:119-23. 2004
    ..The possible role of Gun4 for the Mg as well as Fe porphyrin biosynthesis branches in Synechocystis sp. PCC 6803 is discussed...
  75. ncbi Evaluation of randomly amplified polymorphic DNA and pulsed field gel electrophoresis techniques for molecular typing of Dermatophilus congolensis
    Jose Larrasa
    Departamento de Microbiologia, Laboratorios Larrasa S L, Corredera Hernando de Soto 13 A, Jerez de los Caballeros, 06380 Badajoz, Spain
    FEMS Microbiol Lett 240:87-97. 2004
    ..The results of this study strongly suggest that at least two independent primers are used for RAPD typing in order to improve its discriminatory power, and that PFGE is used for confirmation of RAPD results...
  76. pmc High-density detection of restriction-site-associated DNA markers for rapid mapping of mutated loci in Neurospora
    Zachary A Lewis
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Genetics 177:1163-71. 2007
    ..crassa strains: Mauriceville and Oak Ridge. Furthermore, RAD mapping was used to quickly map a previously unknown gene, defective in methylation-7 (dim-7)...
  77. pmc Geographic diversity among genotypes of Entamoeba histolytica field isolates
    Ali Haghighi
    Department of Parasitology, National Institute of Infectious Diseases, Keio University School of Medicine, Shinjuku ku, Japan
    J Clin Microbiol 41:3748-56. 2003
    ..g., colitis or dysentery and liver abscess, no association between the clinical presentation and the SREHP genotype at either the nucleotide or the predicted amino acid level was demonstrated...
  78. pmc Global methylation screening in the Arabidopsis thaliana and Mus musculus genome: applications of virtual image restriction landmark genomic scanning (Vi-RLGS)
    Tomoki Matsuyama
    Plant Functions Laboratory, RIKEN The Institute of Physical and Chemical Research, Wako, Saitama 351 0198, Japan
    Nucleic Acids Res 31:4490-6. 2003
    ..Vi-RLGS in conjunction with real RLGS will make it possible to develop a more complete map of genomic sites that are methylated or demethylated as a consequence of normal or abnormal development...
  79. pmc Phenotypic and molecular characterization of clinical isolates of Mycobacterium elephantis from human specimens
    Christine Turenne
    National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba
    J Clin Microbiol 40:1230-6. 2002
    ..elephantis, a newly described species isolated from an elephant. All strains were isolated from human samples, 10 from sputum and 1 from an axillary lymph node...
  80. ncbi Combined restriction landmark genomic scanning and virtual genome scans identify a novel human homeobox gene, ALX3, that is hypermethylated in neuroblastoma
    Katharina Wimmer
    Institut für Medizinische Biologie, Universitat Wien, Vienna, Austria
    Genes Chromosomes Cancer 33:285-94. 2002
    ..The VGS approach we have implemented in combination with RLGS is useful for the identification of genomic CpG island-related methylation changes or deletions in cancer...
  81. pmc An algorithm for assembly of ordered restriction maps from single DNA molecules
    Anton Valouev
    Department of Mathematics, University of Southern California, 3620 South Vermont Avenue, KAP 108, Los Angeles, CA 90089 2532, USA
    Proc Natl Acad Sci U S A 103:15770-5. 2006
    The restriction mapping of a massive number of individual DNA molecules by optical mapping enables assembly of physical maps spanning mammalian and plant genomes; however, not through computational means permitting completely de novo ..
  82. pmc Accurate and unambiguous tag-to-gene mapping in serial analysis of gene expression
    Rodrigo Malig
    Departamento de Genetica Molecular y Microbiologia, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Alameda 340, Santiago, Chile
    BMC Bioinformatics 7:487. 2006
    ..The method provides a complete annotation of potential virtual SAGE tags within a genome, along with an estimation of their confidence for experimental observation that ranks tags that present multiple matches in the genome...
  83. pmc An AscI boundary library for the studies of genetic and epigenetic alterations in CpG islands
    Zunyan Dai
    Division of Human Cancer Genetics, Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA
    Genome Res 12:1591-8. 2002
    ..Feasibility of this system is shown in three tumor types, medulloblastomas, lung cancers, and head and neck cancers. We report the cloning of 178 AscI RLGS fragments via two methods by use of this library...
  84. pmc A whole-genome shotgun optical map of Yersinia pestis strain KIM
    Shiguo Zhou
    Laboratory for Molecular and Computational Genomics, University of Wisconsin Madison, 53706, USA
    Appl Environ Microbiol 68:6321-31. 2002
    ..Given the recent advancements to the optical mapping system, increased resolution and throughput are enabling such maps to guide sequence assembly at a very early stage of a microbial sequencing project...
  85. pmc Shotgun optical maps of the whole Escherichia coli O157:H7 genome
    A Lim
    Laboratory for Molecular and Computational Genomics, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
    Genome Res 11:1584-93. 2001
    ..Comparisons were made between maps and sequence data that characterized sequence gaps and guided nascent assemblies...
  86. pmc Virtual genome scan: a tool for restriction landmark-based scanning of the human genome
    J M Rouillard
    Department of Pediatrics, University of Michigan, Ann Arbor 48109, USA
    Genome Res 11:1453-9. 2001
    ....
  87. ncbi A chromosome map of the European stone fruit yellows phytoplasma
    C Marcone
    Biologische Bundesanstalt für Land und Forstwirtschaft, Institut für Pflanzenschutz im Obstbau, D 69221 Dossenheim, Germany
    Microbiology 147:1213-21. 2001
    ..The GSFY1 physical map was different from that of the closely related apple proliferation phytoplasma but the genetic arrangement was similar...
  88. pmc Clinical and epidemiological correlates of genotypes within the Mycobacterium avium complex defined by restriction and sequence analysis of hsp65
    Sandra C Smole
    Department of Medicine, Boston University School of Medicine, VA Boston Healthcare System, Boston, Massachusetts 02130, USA
    J Clin Microbiol 40:3374-80. 2002
    ....
  89. pmc Profiling Mycobacterium ulcerans with hsp65
    Sylvia Cardoso Leao
    Emerg Infect Dis 11:1795-6. 2005
  90. ncbi Methylation and downregulated expression of mac25/insulin-like growth factor binding protein-7 is associated with liver tumorigenesis in SV40T/t antigen transgenic mice, screened by restriction landmark genomic scanning for methylation (RLGS-M)
    S Komatsu
    Core Research for Evolutional Science and Technology CREST of Japan Science and Technology Corporation, Tsukuba Life Science Center, Tsukuba, Ibaraki, Japan
    Biochem Biophys Res Commun 267:109-17. 2000
    ....
  91. ncbi A 5' element of the chicken beta-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila
    J H Chung
    Laboratory of Molecular Biology, National Institutes of Health, Bethesda, Maryland 20892
    Cell 74:505-14. 1993
    ..The action of the insulator thus is not restricted to erythroid or mammalian cells, suggesting that such elements may serve an important and widely distributed function in the organization of chromatin structure...
  92. pmc COBRA: a sensitive and quantitative DNA methylation assay
    Z Xiong
    Department of Surgery, University of Southern California School of Medicine, The Norris Comprehensive Cancer Center, Room 6415, Mailstop 73, 1441 Eastlake Avenue, Los Angeles, CA 90033, USA
    Nucleic Acids Res 25:2532-4. 1997
    ..COBRA thus combines the powerful features of ease of use, quantitative accuracy, and compatibility with paraffin sections...
  93. ncbi Mutations in Hsp83 and cdc37 impair signaling by the sevenless receptor tyrosine kinase in Drosophila
    T Cutforth
    Howard Hughes Medical Institute, University of California, Berkeley 94720 3200
    Cell 77:1027-36. 1994
    ..Together, these data support a role for Hsp90 proteins in tyrosine kinase regulation and suggest that signals promoting neuronal differentiation may involve cell cycle control...
  94. pmc Suppression of a sec63 mutation identifies a novel component of the yeast endoplasmic reticulum translocation apparatus
    T Kurihara
    Department of Molecular Biology, Princeton University, New Jersey 08544
    Mol Biol Cell 4:919-30. 1993
    ..We propose that HSS1p is important for ER translocation and interacts with previously identified components of the yeast translocation apparatus. HSS1 is identical to SEC66, which encodes a glycoprotein complexed with SEC62p and SEC63p...
  95. pmc The kinesin-like protein KLP61F is essential for mitosis in Drosophila
    M M Heck
    Johns Hopkins University, School of Medicine, Department of Cell Biology and Anatomy, Baltimore, Maryland 21205
    J Cell Biol 123:665-79. 1993
    ....
  96. pmc Molecular complementation of a genetic marker in Dictyostelium using a genomic DNA library
    J L Dynes
    Department of Biology, University of California, San Diego, La Jolla 92093
    Proc Natl Acad Sci U S A 86:7966-70. 1989
    ..In addition, this work suggests that it will be possible to isolate genes that are essential for developmental processes in Dictyostelium by complementation...
  97. pmc Identification, cloning, and sequencing of a fragment of Amsacta moorei entomopoxvirus DNA containing the spheroidin gene and three vaccinia virus-related open reading frames
    R L Hall
    Department of Immunology and Medical Microbiology, University of Florida, Gainesville 32610 0266
    J Virol 65:6516-27. 1991
    ..An analysis of open reading frames around the spheroidin gene suggests that the colinear core of "essential genes" typical of the vertebrate poxviruses is absent in A. moorei entomopoxvirus...
  98. ncbi DNA sequence analysis of conserved and unique regions of swinepox virus: identification of genetic elements supporting phenotypic observations including a novel G protein-coupled receptor homologue
    R F Massung
    Department of Immunology and Medical Microbiology, JHMHC, University of Florida College of Medicine, Gainesville 32610
    Virology 197:511-28. 1993
    ..The lack of these functional homologues could explain the characteristic attenuated phenotype and limited host range of SPV...
  99. ncbi Identification of a retinoic acid responsive element in the retinoic acid receptor beta gene
    H De The
    Unité de recombinaison et d expression génétique INSERM U 163, CNRS UA 271, Institut Pasteur, Paris
    Nature 343:177-80. 1990
    ..Both alpha and beta receptors enhance retinoic acid response in CV1 cells, indicating that they can both act through the same DNA sequence...
  100. ncbi DPY-27:a chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome
    P T Chuang
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Cell 79:459-74. 1994
    ..We propose that DPY-27 implements dosage compensation by condensing the chromatin structure of X in a manner that causes general reduction of X chromosome expression...
  101. ncbi A new Drosophila homeo box gene is expressed in mesodermal precursor cells of distinct muscles during embryogenesis
    C Dohrmann
    Max Planck Institut fur Entwicklungsbiologie, Abteilung III Genetik, Tubingen, Germany
    Genes Dev 4:2098-111. 1990
    ..In addition to the mesoderm, S59 is expressed in a subset of neuronal cells of the CNS and their precursors and also in cells of a small region of the midgut...

Research Grants62

  1. Nanofluidic Platforms for High Resolution Mapping of Genomic DNA
    JOHN MICHAEL RAMSEY; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): We propose to develop a nanofluidic platform for the high-throughput restriction mapping of complete genomes...
  2. STRUCTURE AND GENETIC CONTROL OF IGA
    Katherine Knight; Fiscal Year: 2000
    ..From restriction mapping and nucleotide sequence analysis of these genes one can determine whether the trans molecules result from ..
  3. HEX EXPRESSION AND REGULATION IN DEVELOPING LUNG
    CLIFFORD BOGUE; Fiscal Year: 2000
    ..The methodology to be used to complete these specific aims includes: In situ hybridization, isolation and restriction mapping of genomic clones for Hex; identification of enhancer elements using reporter gene analysis in transgenic ..
  4. GENETIC ANALYSIS OF CHARCOT-MARIE-TOOTH DISEASE
    PHILLIP CHANCE; Fiscal Year: 1992
    ..to define the molecular defect(s) in HMSNIb, including high resolution chromosomal analysis and linkage and restriction mapping of cDNAs which map to the immediate area of the HMSNIb locus...
  5. ANOXIA RESPONSIVE VL30 ELEMENTS
    Garth Anderson; Fiscal Year: 1992
    ..3. With VL30 representing a multigene family with 50-100 copies per cell in rat DNA, we will use restriction mapping of cDNA clones to determine if only one or many of these elements are expressed in response to anoxia. 4...
  6. MECHANISMS & REGULATION OF PROTEIN SYNTHESIS
    ALBERT WAHBA; Fiscal Year: 1992
    ..The isolated clones will be characterized by restriction mapping, nucleotide sequencing, and their derived amino acid sequence. The sequencing of the genomic clones for
  7. AMPLIFICATION--MODEL FOR GENETIC INSTABILITY IN CANCER
    JOYCE HAMLIN; Fiscal Year: 2005
    ..then be determined, and rearrangements will be analyzed by fluorescence in situ hybridization (FISH) and restriction mapping; 3) to determine the extent to which break-induced replication can occur in CHO cells, as a possible model ..
  8. MUTAGENIC AND RECOMBINOGENIC EFFECTS OF GAMMA RAYS
    William Taylor; Fiscal Year: 1990
    ....
  9. IN VIVO EXPRESSION OF SCHWANN CELL NA CHANNEL
    GING WANG; Fiscal Year: 1990
    ..The isolated cDNA clones can then be characterized by restriction mapping and partial cDNA sequencing, so that the type of Schwann cell Na channels can be compared with the rat ..
  10. CLONING OF THE INVASIVENESS GENE FROM BARTONELLA BACILLI
    ETHELEEN MC GINNIS; Fiscal Year: 1993
    ..techniques and erythrocyte-binding assays; (5) Analysis of the structure of the invasiveness gene using restriction mapping and S1 nuclease analysis and (6) Determination of the nucleotide sequence at the 5' end of the invasiveness ..
  11. NIH Biotechnologist: Authentic Exploration of Biotechnology in Health Science
    Steven Moore; Fiscal Year: 2007
    ..of Biotechnology, Genetics, and Genomics in Health Science project will produce a model lesson-"Restriction Mapping: Mapping restriction sites on a plasmid"-derived from the Biotechnologist instructional materials developed ..
  12. CLONING AND CHARACTERIZATION OF THE RETINOBLASTOMA GENE
    YUEN FUNG; Fiscal Year: 1991
    ..2) Restriction mapping of the genomic clones...
  13. PHYSICAL MAP & ORDERED COSMID SET: HUMAN CHROMOSOME 11
    GLEN EVANS; Fiscal Year: 1990
    ..for the construction of contigs using (i) multidimensional cosmid multiplex analysis and (ii) detailed restriction mapping of a large portion of the set by automated DNA preparation and digestion, (4) locating mapping landmarks ..
  14. REGULATION & FUNCTION OF CALCIUM TRANSPORTING ATPASES
    GARY SHULL; Fiscal Year: 1993
    ..The genes will be characterized by restriction mapping, blot hybridization and DNA sequence analysis...
  15. GENOMIC MAPPING
    KENNETH TARTOF; Fiscal Year: 1991
    ..is the demonstration of substantial homologous overlap as can be most readily obtained by intensive restriction mapping. We have already developed lambda phage, plasmid and cosmid vectors and techniques that allow one to easily ..
  16. RENAL DOPAMINE RECEPTORS AND INHIBITION OF NA,K-ATPASE
    Istvan Seri; Fiscal Year: 1993
    ..with advanced recombinant DNA techniques and strategies such as the polymerase chain reaction (CR), restriction mapping and sequencing, and screening under low and high stringency conditions to clone and sequence the peripheral ..
  17. CATECHOL-O-METHYLTRANSFERASE: BIOCHEMISTRY AND GENETICS
    Mark Grossman; Fiscal Year: 1991
    ..The genomic clones will be characterized by restriction mapping, nucleotide sequencing, and S-1 mapping to determine the intron and exon positions...
  18. EXPRESSION AND REGULATION OF THE CATECHOLAMINE GENES
    Margaret Ross; Fiscal Year: 1990
    ..detailed analyses of the full length cDNAs and genomic clones of TH, DBH and PNMT will be performed using restriction mapping, S1 nuclease mapping and DNA sequencing techniques...
  19. RECOGNITION OF LEUKEMIA ANTIGEN BY ANTI-VIRAL CTL'S
    HILLARY WHITE; Fiscal Year: 1991
    ..To further study viral epitope expression for recognition by CTL, I propose here to clone and compare by restriction mapping the Akv-1 and Akv-4 proviral DNA loci...
  20. HUMAN DESMOPLAKIN GENE STRUCTURE & EXPRESSION IN EMBRYOS
    Kathleen Green; Fiscal Year: 1990
    ..The structure of the human DP cDNAs (mRNAs) will be determined by restriction mapping, cross- hybridization experiments and DNA sequence analysis...
  21. REGULATION OF IL 12 IN MUCOSAL IMMUNITY AND IBD
    Scott Plevy; Fiscal Year: 1999
    ..region of each gene will be analyzed from murine genomic DNA clones by transcription start site mapping, restriction mapping, and DNA sequencing...
  22. CHROMOSOMAL TRANSLOCATIONS IN CHILDHOOD ACUTE LEUKEMIA
    Nora Heisterkamp; Fiscal Year: 1990
    ..In addition, the proposed research might shed light on the molecular significance of the bcr part of the bcr/ ABL protein, which represents the hallmark of CML...
  23. BIOLOGICAL CHARACTERIZATION OF HSV INVASIVENESS GENES
    Jesse Goodman; Fiscal Year: 1993
    ..Extensive fine restriction mapping of these virulent recombinants reveals an ANG DNA insert within a 3.7 kb region (0.334-0...
  24. STRUCTURAL ANALYSIS OF I REGION VARIANCE
    Edward Wakeland; Fiscal Year: 1993
    ..High resolution restriction mapping will be used to assess sequence diversification patterns over the region encompassing the A alpha, A beta, ..
  25. MOLECULAR GENETICS OF HUMAN RED BLOOD CELL SPECTRIN
    JOHN WINKELMANN; Fiscal Year: 1992
    ..I will isolate the alpha and beta spectrin gene promoters by molecular cloning. These will be analyzed by restriction mapping and nucleotide sequencing...
  26. MECHANISM OF ESTROGEN ACTION IN THE ENDOCERVIX
    Beverly Chilton; Fiscal Year: 1992
    ..These clones will be characterized by hybridization selection, and restriction mapping to determine whether there are different species of mRNA that code for different mucin proteins...
  27. ANTITERMINATION AND CELLULAR GENE EXPRESSION
    Catherine Squires; Fiscal Year: 1993
    ..Our initial characterizations will continue to be t e following: 1) Quick restriction mapping to size the insert and make sure t e terminator is intact...
  28. VH REGION DIVERSIFICATION AMONG HAPLOTYPES
    Honghua Li; Fiscal Year: 2003
    ..physical structure of each haplotype will be examined to identify the regions of genetic interest after restriction mapping. The sequences of the identified regions will be determined...
  29. EXPRESSION OF AVIAN SARCOMA VIRUS IN RAT FIBROBLASTS
    ERIC HUMPHRIES; Fiscal Year: 1980
    ..The loci will be cloned and analyzed with respect to the viral sequence content (RAV-O related sequences) and the non-viral flanking sequences (non-RAV-O related)...
  30. GLOBIN MRNA: DETERMINATION OF REGULATING SEQUENCES
    GARY PADDOCK; Fiscal Year: 1980
    ..This second phase of recombinant DNAs will be studied by restriction enzyme analysis, R-loop analysis, and nucleotide sequence analysis. ..
  31. PHYSICAL MAP OF HUMAN CHROMOSOME 18
    Uta Francke; Fiscal Year: 1993
    ..The 18-only hybrid cell line will be used for large scale restriction mapping by pulsed field gel electrophoresis (PFGE), and for the generation of chromosome-18-specific cosmid and ..
  32. CHARACTERIZATION OF A TRYPANSOMA BRUCEI PROTEASE
    Eric Pamer; Fiscal Year: 1991
    ..The gene and its flanking sequences will be cloned and characterized by restriction mapping and sequencing. This will allow comparison of the trypanosomal enxzyme with other known cysteine proteases...
  33. MOLECULAR GENETICS OF HUMAN LEUKEMIA AND LYMPHOMA
    TIMOTHY MEEKER; Fiscal Year: 1991
    ..The genomic and cDNA clones of these genes (bcl-2 and bcl-3) will then be characterized by restriction mapping, S1 mapping and sequencing...
  34. Comparison of CYP2 Genes Across Mammal Species
    Susan Hoffman; Fiscal Year: 2004
    ..A detailed physical map of the CYP2 gene cluster will be created for each species by isolating and restriction mapping genomic clones from the appropriate BAC libraries...
  35. EPIDEMIOLOGY OF CYTOMEGALOVIRUS IN DAY CARE CENTERS
    Robert Pass; Fiscal Year: 1992
    ..By examining this population of normal hosts and determining to what degree natural infection protects from reinfection, knowledge will be acquired that will be critical in assessing a potential candidate vaccine...
  36. HUMAN ISOFERRITINS STRUCTURE AND METABOLISM
    JAMES DRYSDALE; Fiscal Year: 1990
    ..The organization and structure of expressed genes will be determined by use of restriction mapping and hybridization of the genomic clones to appropriate sub-probes, R loop and heteroduplex mapping, and ..
  37. USE OF ANTI-IA ANTISERA IN MURINE SYSTEMIC LUPUS
    Hugh McDevitt; Fiscal Year: 1992
    ..Genomic clones will be analyzed by restriction mapping in a search for differences between the 5' flanking sequences, intervening sequences, and 3' flanking ..
  38. THE MOLECULAR GENETICS OF CYSTIC FIBROSIS
    HAIG KAZAZIAN; Fiscal Year: 1990
    ..an affected individual with a chromosomal deletion or rearrangement involving the CF gene using macro- restriction mapping using the tightly linked, flanking DNA probes Met and D7S8 (J3.11)...
  39. GENE REGULATION--SMOOTH MUSCLE REGULATORY LIGHT CHAIN
    James Grant; Fiscal Year: 1991
    ..The structural organization of the gene will be defined by a combination of sequencing and restriction mapping. A variety of tissues including smooth muscle, non-muscles and straited muscles will be examined by ..
  40. NEW METHODS FOR MANIPULATION OF THE DROSOPHILA GENOME
    Dan Garza; Fiscal Year: 2001
    ..excision/deletion events analyzed genetically by using complementation tests, and molecularly by long-range restriction mapping and in situ hybridization...
  41. GENETIC MARKERS, LEUKEMOGENESIS AND THYMIC FUNCTION
    Paul Gottlieb; Fiscal Year: 1992
    ..Information on the nature of the polymorphism has come from cloning, restriction mapping and nucleotide sequence analysis of allelic members of gene families from inbred and wild mice...
  42. PHYSICAL MAPPING OF AN ARCHAEBACTERIAL GENOME
    Neil Hackett; Fiscal Year: 1992
    ..Two methods will be combined to obtain a chromosomal map for each strain. The first is low resolution restriction mapping of uncloned chromosomal DNA digested with enzymes that cut infrequently...
  43. REGULATION OF NA, K-ATPASE EXPRESSION IN KIDNEY CELLS
    JESSE BOWEN; Fiscal Year: 1992
    ..coding regions and transcription regulating regions of the alpha and beta subunit DNA will be examined by restriction mapping and/or sequencing DNA fragments isolated from cDNA or genomic libraries prepared from the cell lines...
  44. ANALYSIS AND CLONING OF A DEVELOPMENTAL SKIN LOCUS
    JEROME GORSKI; Fiscal Year: 1990
    ..among the IP translocations by Southern blot analysis and pulse field gradient electrophoretic restriction mapping techniques. X chromosomal DNA fragments from the delineated Ip locus will be cloned...
  45. MICROCLONING OF THE DROSOPHILA GENOME
    Vincenzo Pirrotta; Fiscal Year: 1991
    ..The overlaps between cosmids from each microdissected region will be established by restriction mapping. For the majority of regions, it is likely that the overlapping cosmids will define a single continuous ..
  46. CELLULAR TROPISM OF HIV
    Roger Pomerantz; Fiscal Year: 1992
    ..During this time, the candidate will become familiar with a number of important and generalizable molecular virologic techniques...
  47. SOLID STATE DNA SIZING
    JAN HOH; Fiscal Year: 1999
    ..Conventional restriction mapping reveals a sequence dependent distribution of restriction sites, and is an indirect form of DNA decoration...
  48. Gamma globin is silenced by chromatin changes
    Steven Fiering; Fiscal Year: 2006
    ..abstract_text> ..
  49. CLONING LARGE FRAGMENTS OF CHROMOSOME THREE DNA
    Katheleen Gardiner; Fiscal Year: 1992
    ..Pulsed field gel analysis will allow long range restriction mapping around regionally mapped probes; yeast artificial chromosome will allow the isolation, in a single clone, ..
  50. MACRORESTRICTION MAPPING APPLIED TO CHROMOSOME 5Q
    CAROL WESTBROOK; Fiscal Year: 1990
    ..The map will consist of unique probes of known order and distance that have been linked by megabase restriction mapping. Emphasis will be placed on bands 5423-31, an area of biomedical significance, containing genes that are ..
  51. RADIOANALYTIC IMAGING SYSTEM
    PAUL ROHEIM; Fiscal Year: 1991
    ..b) To facilitate rapid analysis of DNA sequencing, restriction mapping, message quantitation, etc. This instrument greatly improves the accuracy and the speed of evaluation...
  52. CONSTRUCTION OF AN AUTOMATED PLASMID PREP INSTRUMENT
    EDWARD HYMAN; Fiscal Year: 1993
    ..coli for subsequent DNA sequencing and restriction mapping. I have developed a radically new procedure for the preparation of plasmid DNA called Sequential Enzymatic ..
  53. GENETIC STUDIES OF NORTH CAROLINA MACULAR DYSTROPHY
    Kent Small; Fiscal Year: 1993
    ..Once linkage to a chromosome is found, fine mapping techniques (macro restriction mapping, field inversion gel electrophoresis, detection of Hpall tiny fragment islands, yeast artificial ..
  54. Molecular Epidemiology of Community-Associated MRSA
    SANJAY SHUKLA; Fiscal Year: 2008
    ..We will determine the presence or absence of 40 different virulence factor genes in all 320 isolates listed in aim 2 and determine evolution of virulence factor genes in contemporary CA-MRSA. ..
  55. CONVERGENT MECHANISMS CONTRIBUTING TO CANCER
    Joseph Costello; Fiscal Year: 2006
    ....
  56. Genomics, Cis-Regulation and Genetic Variation
    Richard Karp; Fiscal Year: 2005
    ..To foster interdisciplinary training and collaboration, students and post-docs in this program will work with dual mentors. ..
  57. Virulence-conferring siderophore biosynthesis inhibitors
    LUIS QUADRI; Fiscal Year: 2006
    ..These antimicrobials will constitute a base for subsequent studies for the development of new drugs that, alone or in combination therapies, are anticipated to be useful in the treatment of MDR Yp and Mb infections. ..
  58. Gravitaxic behavior in Drosophila
    KATHLEEN BECKINGHAM; Fiscal Year: 2003
    ..are used to sense gravity? iii) What role does light play in modulating gravitaxic responses? iv) What molecular signaling pathways are used in the processing of gravitational information in the peripheral and central nervous systems? ..
  59. Array-based methylation analysis using anti-5mC antibody
    Fumiichiro Yamamoto; Fiscal Year: 2007
    ..We will evaluate the sensitivity, specificity, and reproducibility of the method, using BAG clone microarrays, DNA fragment microarrays, and oligonucleotide microarrays. [unreadable] [unreadable] [unreadable]..
  60. Obesity, Inflammation and Thrombosis: LOOK AHEAD
    Christie M Ballantyne; Fiscal Year: 2010
    ....
  61. EFFECTS OF CHROMATIN ARCHITECTURE ON ORIGIN ACTIVITY
    PIETER DIJKWEL; Fiscal Year: 2003
    ..abstract_text> ..