reverse transcriptase polymerase chain reaction

Summary

Summary: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.

Top Publications

  1. pmc Real-time quantification of microRNAs by stem-loop RT-PCR
    Caifu Chen
    Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA
    Nucleic Acids Res 33:e179. 2005
  2. pmc A microRNA signature of hypoxia
    Ritu Kulshreshtha
    Molecular Oncology Research Institute, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Mol Cell Biol 27:1859-67. 2007
  3. pmc MicroRNA expression, survival, and response to interferon in liver cancer
    Junfang Ji
    Liver Carcinogenesis Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    N Engl J Med 361:1437-47. 2009
  4. ncbi Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
    K J Livak
    Applied Biosystems, Foster City, California 94404, USA
    Methods 25:402-8. 2001
  5. pmc A new mathematical model for relative quantification in real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 29:e45. 2001
  6. pmc Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    Jo Vandesompele
    Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Biol 3:RESEARCH0034. 2002
  7. ncbi Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    Claus Lindbjerg Andersen
    Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Skejby, DK 8200 Aarhus N, Denmark
    Cancer Res 64:5245-50. 2004
  8. ncbi Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
    Xi Chen
    Jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, China
    Cell Res 18:997-1006. 2008
  9. pmc Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    Michael W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 30:e36. 2002
  10. pmc Obesity is associated with macrophage accumulation in adipose tissue
    Stuart P Weisberg
    Division of Moleuclar Genetics, Department of Pediatrics, Columbia University, New York, New York 10032, USA
    J Clin Invest 112:1796-808. 2003

Detail Information

Publications309 found, 100 shown here

  1. pmc Real-time quantification of microRNAs by stem-loop RT-PCR
    Caifu Chen
    Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA
    Nucleic Acids Res 33:e179. 2005
    ..Furthermore, the concept of stem-loop RT primer design could be applied in small RNA cloning and multiplex assays for better specificity and efficiency...
  2. pmc A microRNA signature of hypoxia
    Ritu Kulshreshtha
    Molecular Oncology Research Institute, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Mol Cell Biol 27:1859-67. 2007
    ..Interestingly, the vast majority of hypoxia-induced microRNAs are also overexpressed in a variety of human tumors...
  3. pmc MicroRNA expression, survival, and response to interferon in liver cancer
    Junfang Ji
    Liver Carcinogenesis Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    N Engl J Med 361:1437-47. 2009
    ..Hepatocellular carcinoma is a common and aggressive cancer that occurs mainly in men. We examined microRNA expression patterns, survival, and response to interferon alfa in both men and women with the disease...
  4. ncbi Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
    K J Livak
    Applied Biosystems, Foster City, California 94404, USA
    Methods 25:402-8. 2001
    ..In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data...
  5. pmc A new mathematical model for relative quantification in real-time RT-PCR
    M W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 29:e45. 2001
    ..High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model...
  6. pmc Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    Jo Vandesompele
    Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Biol 3:RESEARCH0034. 2002
    ....
  7. ncbi Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    Claus Lindbjerg Andersen
    Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Skejby, DK 8200 Aarhus N, Denmark
    Cancer Res 64:5245-50. 2004
    ..The presented strategy can be applied to evaluate the suitability of any normalization gene candidate in any kind of experimental design and should allow more reliable normalization of RT-PCR data...
  8. ncbi Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
    Xi Chen
    Jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, China
    Cell Res 18:997-1006. 2008
    ..Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases...
  9. pmc Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    Michael W Pfaffl
    Institute of Physiology, FML Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Germany
    Nucleic Acids Res 30:e36. 2002
    ..The latest software version of REST and examples for the correct use can be downloaded at http://www.wzw.tum.de/gene-quantification/...
  10. pmc Obesity is associated with macrophage accumulation in adipose tissue
    Stuart P Weisberg
    Division of Moleuclar Genetics, Department of Pediatrics, Columbia University, New York, New York 10032, USA
    J Clin Invest 112:1796-808. 2003
    ..Adipose tissue macrophage numbers increase in obesity and participate in inflammatory pathways that are activated in adipose tissues of obese individuals...
  11. ncbi Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations
    Michael W Pfaffl
    Physiology, FML Weihenstephan, Centre of Life and Food Science, Technical University of Munich, Germany
    Biotechnol Lett 26:509-15. 2004
    ..The BestKeeper application and necessary information about data processing and handling can be downloaded on http://www.wzw.tum.de/gene-quantification/bestkeeper.html...
  12. pmc Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance
    Haiyan Xu
    Millennium Pharmaceuticals, Cambridge, Massachusetts, USA
    J Clin Invest 112:1821-30. 2003
    ..We propose that obesity-related insulin resistance is, at least in part, a chronic inflammatory disease initiated in adipose tissue...
  13. ncbi Unique microRNA molecular profiles in lung cancer diagnosis and prognosis
    Nozomu Yanaihara
    Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cancer Cell 9:189-98. 2006
    ..These results indicate that miRNA expression profiles are diagnostic and prognostic markers of lung cancer...
  14. pmc Human adipose tissue is a source of multipotent stem cells
    Patricia A Zuk
    Department of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Biol Cell 13:4279-95. 2002
    ..Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression...
  15. pmc Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia
    George Adrian Calin
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 99:15524-9. 2002
    ..Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases...
  16. ncbi A multigene assay to predict recurrence of tamoxifen-treated, node-negative breast cancer
    Soonmyung Paik
    Division of Pathology, Operation Center, and the Biostatistics Center, National Surgical Adjuvant Breast and Bowel Project, Pittsburgh 15212, USA
    N Engl J Med 351:2817-26. 2004
    ..The likelihood of distant recurrence in patients with breast cancer who have no involved lymph nodes and estrogen-receptor-positive tumors is poorly defined by clinical and histopathological measures...
  17. pmc Obesity induces a phenotypic switch in adipose tissue macrophage polarization
    Carey N Lumeng
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Clin Invest 117:175-84. 2007
    ..Thus, diet-induced obesity leads to a shift in the activation state of ATMs from an M2-polarized state in lean animals that may protect adipocytes from inflammation to an M1 proinflammatory state that contributes to insulin resistance...
  18. ncbi Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London E1 1BB, UK
    J Mol Endocrinol 25:169-93. 2000
    ..It illustrates the usefulness of these assays by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH)...
  19. pmc Increased oxidative stress in obesity and its impact on metabolic syndrome
    Shigetada Furukawa
    Department of Medicine and Pathophysiology, Graduate School of Medicine, Department of Organismal Biosystems, Graduate School of Frontier Biosciences, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Clin Invest 114:1752-61. 2004
    ....
  20. pmc Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
    J M Ruijter
    Heart Failure Research Center, Academic Medical Center, University of Amsterdam, The Netherlands
    Nucleic Acids Res 37:e45. 2009
    ..The variability, as well as the bias, in qPCR results was significantly reduced when the mean of these PCR efficiencies per amplicon was used in the calculation of an estimate of the starting concentration per sample...
  21. ncbi Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening
    E K O Ng
    Institute of Digestive Disease, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Gut 58:1375-81. 2009
    ..MicroRNAs (miRNAs) have been shown to offer great potential in the diagnosis of cancer. We investigated whether plasma miRNAs could discriminate between patients with and without colorectal cancer (CRC)...
  22. ncbi microRNAs as oncogenes and tumor suppressors
    Baohong Zhang
    Department of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79409 1163, USA
    Dev Biol 302:1-12. 2007
    ..miRNA expression profiles may become useful biomarkers for cancer diagnostics. In addition, miRNA therapy could be a powerful tool for cancer prevention and therapeutics...
  23. pmc Serum microRNAs are promising novel biomarkers
    Shlomit Gilad
    Rosetta Genomics Ltd, Rehovot, Israel
    PLoS ONE 3:e3148. 2008
    ..Here we show that microRNAs, a family of small non-coding regulatory RNAs involved in human development and pathology, are present in bodily fluids and represent new effective biomarkers...
  24. pmc A novel and universal method for microRNA RT-qPCR data normalization
    Pieter Mestdagh
    Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, Ghent, Belgium
    Genome Biol 10:R64. 2009
    ..We demonstrate that the mean expression value outperforms the current normalization strategy in terms of better reduction of technical variation and more accurate appreciation of biological changes...
  25. pmc MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma
    Aaron J Schetter
    Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    JAMA 299:425-36. 2008
    ..MicroRNAs have potential as diagnostic biomarkers and therapeutic targets in cancer. No study has evaluated the association between microRNA expression patterns and colon cancer prognosis or therapeutic outcome...
  26. ncbi IRF-7 is the master regulator of type-I interferon-dependent immune responses
    Kenya Honda
    Department of Immunology, Graduate School of Medicine and Faculty of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Nature 434:772-7. 2005
    ..Thus, all elements of IFN responses, whether the systemic production of IFN in innate immunity or the local action of IFN from plasmacytoid dendritic cells in adaptive immunity, are under the control of IRF-7...
  27. ncbi Quantitative real-time RT-PCR--a perspective
    S A Bustin
    Institute of Cellular and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK
    J Mol Endocrinol 34:597-601. 2005
    ....
  28. pmc Statistical analysis of real-time PCR data
    Joshua S Yuan
    Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA
    BMC Bioinformatics 7:85. 2006
    ..Based on the standard curve method and other useful data analysis methods, we present and compare four statistical approaches and models for the analysis of real-time PCR data...
  29. pmc Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR
    Nicholas Silver
    Molecular Haematology, Division of Gene and Cell Based Therapy, King s College London School of Medicine at King s College Hospital, Denmark Hill, London, SE5 9PJ, UK
    BMC Mol Biol 7:33. 2006
    ..On this basis, stability of the candidate housekeeping genes was ranked according to repeatability of the gene expression differences among 31 samples...
  30. ncbi Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1
    Daniel J Ceradini
    Laboratory of Microvascular Research and Vascular Tissue Engineering, Institute of Reconstructive Plastic Surgery, New York University School of Medicine, New York, New York 10016, USA
    Nat Med 10:858-64. 2004
    ..These data show that the recruitment of CXCR4-positive progenitor cells to regenerating tissues is mediated by hypoxic gradients via HIF-1-induced expression of SDF-1...
  31. pmc Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes
    Erica Spackman
    Southeast Poultry Research Laboratory, USDA Agricultural Research Service, Athens, Georgia 30605, USA
    J Clin Microbiol 40:3256-60. 2002
    ..The remaining samples were positive with only one detection method. Overall the sensitivity and specificity of the H7- and H5-specific RRT-PCR were similar to those of VI and HI...
  32. ncbi A plant miRNA contributes to antibacterial resistance by repressing auxin signaling
    Lionel Navarro
    Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Science 312:436-9. 2006
    ..Repression of auxin signaling restricts P. syringae growth, implicating auxin in disease susceptibility and miRNA-mediated suppression of auxin signaling in resistance...
  33. ncbi Universal primer set for the full-length amplification of all influenza A viruses
    E Hoffmann
    Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105-2794, USA
    Arch Virol 146:2275-89. 2001
    ..The resultant primer set is suitable for all influenza A viruses to generate full-length cDNAs, to subtype viruses, to sequence their DNA, and to construct expression plasmids for reverse genetics systems...
  34. ncbi Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress
    Nathalie Nicot
    Centre de Recherche Public Gabriel Lippmann, Environment and Biotechnologies Research Unit, 41 rue du Brill, L 4422 Belvaux, Luxembourg
    J Exp Bot 56:2907-14. 2005
    ..The relative quantification of the hsp20.2 gene varied according to the internal control and the number of internal controls used, thus highlighting the importance of the choice of internal controls in such experiments...
  35. ncbi Impaired microRNA processing enhances cellular transformation and tumorigenesis
    Madhu S Kumar
    MIT Center for Cancer Research, Cambridge, Massachusetts 02139, USA
    Nat Genet 39:673-7. 2007
    ..Furthermore, conditional deletion of Dicer1 enhanced tumor development in a K-Ras-induced mouse model of lung cancer. Overall, these studies indicate that abrogation of global miRNA processing promotes tumorigenesis...
  36. pmc Comprehensive algorithm for quantitative real-time polymerase chain reaction
    Sheng Zhao
    Department of Biological Sciences and Program in Neuroscience, Stanford University, Stanford, California 94305 5020, USA
    J Comput Biol 12:1047-64. 2005
    ..This algorithm provides an objective and noise-resistant method for quantification of qRT-PCR results that is independent of the specific equipment used to perform PCR reactions...
  37. pmc Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature
    Emmanuelle Charafe-Jauffret
    Centre de Recherche en Cancerologie de Marseille, Laboratoire d Oncologie Moleculaire, UMR891 Inserm Institut Paoli Calmettes, Universite de la Mediterranee, Marseille, France
    Cancer Res 69:1302-13. 2009
    ....
  38. pmc Systematic comparison of microarray profiling, real-time PCR, and next-generation sequencing technologies for measuring differential microRNA expression
    Anna Git
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, United Kingdom
    RNA 16:991-1006. 2010
    ..Finally, we implemented a novel method to evaluate false-positive and false-negative rates for all methods in the absence of a reference method...
  39. pmc Regulation of the p27(Kip1) tumor suppressor by miR-221 and miR-222 promotes cancer cell proliferation
    Carlos le Sage
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    EMBO J 26:3699-708. 2007
    ..Thus, deregulated expression of miR-221&222 promotes cancerous growth by inhibiting the expression of p27(Kip1)...
  40. pmc Defining the epithelial stem cell niche in skin
    Tudorita Tumbar
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY 10021, USA
    Science 303:359-63. 2004
    ..Many of the >100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment...
  41. pmc Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process
    Marino Expósito-Rodríguez
    Instituto de Productos Naturales y Agrobiología CSIC, Avda, Astrofísico Francisco Sánchez 3, 38206 La Laguna, Tenerife, Canary Islands, Spain
    BMC Plant Biol 8:131. 2008
    ..In the present report we describe the first systematic evaluation of potential internal controls during tomato development process to identify which are the most reliable for transcript quantification by real-time RT-PCR...
  42. ncbi Serum microRNA profiles serve as novel biomarkers for HBV infection and diagnosis of HBV-positive hepatocarcinoma
    Li Min Li
    Institute for Virology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China
    Cancer Res 70:9798-807. 2010
    ..96 (specificity: 96%; sensitivity: 100%) in HCC prediction. In conclusion, our study demonstrates for the first time that serum miRNA profiles can serve as novel and noninvasive biomarkers for HBV infection and HBV-positive HCC diagnosis...
  43. pmc Normalization of microRNA expression levels in quantitative RT-PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid tissues
    Heidi J Peltier
    Asuragen, Inc, Austin, Texas 78744, USA
    RNA 14:844-52. 2008
    ..These findings constitute the first report describing the rigorous normalization of miRNA qRT-PCR data and have important implications for proper experimental design and accurate data interpretation...
  44. ncbi Twenty-five years of quantitative PCR for gene expression analysis
    Heather D VanGuilder
    Department of Pharmacology, Penn State College of Medicine, Hershey, PA, USA
    Biotechniques 44:619-26. 2008
    ..The future of qPCR remains bright as the technology becomes more rapid, cost-effective, easier to use, and capable of higher throughput...
  45. pmc Circulating microRNAs in plasma of patients with gastric cancers
    M Tsujiura
    Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, 465 Kajii cho, Kawaramachihirokoji, Kamigyo ku, Kyoto 602 8566, Japan
    Br J Cancer 102:1174-9. 2010
    ..We examined plasma microRNA (miRNA) concentrations from patients with gastric cancers (GCs) to assess their clinical application for diagnosing and monitoring diseases...
  46. pmc Dicer, Drosha, and outcomes in patients with ovarian cancer
    William M Merritt
    University of Texas M D Anderson Cancer Center, Houston, TX 77230, USA
    N Engl J Med 359:2641-50. 2008
    ..We studied Dicer and Drosha, components of the RNA-interference machinery, in ovarian cancer...
  47. ncbi Real-time RT-PCR normalisation; strategies and considerations
    J Huggett
    Centre for Infectious Diseases and International Health, University College London, London, UK
    Genes Immun 6:279-84. 2005
    ..In this review we discuss the relative merits of different normalisation strategies and suggest a method of validation that will enable the measurement of biologically meaningful results...
  48. pmc An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues
    Chang Gong Liu
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 101:9740-4. 2004
    ..The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states...
  49. ncbi The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants
    Laurent Gutierrez
    Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 901 83 Umea, Sweden
    Plant Biotechnol J 6:609-18. 2008
    ..For the first time, the extent to which the lack of a systematic validation of reference genes is a stumbling block to the reliability of results obtained by RT-PCR in plants is clearly shown...
  50. pmc Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
    Stephen A Bustin
    Centre for Academic Surgery Institute of Cell and Molecular Science Barts, University of London, UK
    J Biomol Tech 15:155-66. 2004
    ....
  51. ncbi Quantitative real-time RT-PCR data analysis: current concepts and the novel "gene expression's CT difference" formula
    Jan H Schefe
    Center for Cardiovascular Research CCR Institute of Pharmacology and Toxicology, Charite Universitatsmedizin Berlin, Hessische Strasse 3 4, 10115, Berlin, Germany
    J Mol Med (Berl) 84:901-10. 2006
    ..Additionally, this article offers some technical considerations and information on statistical analysis of real-time PCR data...
  52. ncbi Quantification of mRNA using real-time RT-PCR
    Tania Nolan
    Sigma Aldrich, Homefield Road, Haverhill, UK
    Nat Protoc 1:1559-82. 2006
    ..The entire protocol described here, encompassing all stages from initial assay design to reliable qPCR data analysis, requires approximately 15 h...
  53. ncbi Detection and characterization of placental microRNAs in maternal plasma
    Stephen S C Chim
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China
    Clin Chem 54:482-90. 2008
    ..Because nucleic acids of placental origin are released into maternal plasma, we hypothesized that miRNAs produced by the placenta would also be released into maternal plasma...
  54. ncbi Epigenetic inactivation of SFRP genes allows constitutive WNT signaling in colorectal cancer
    Hiromu Suzuki
    Division of Tumor Biology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Medical Institutions, 1650 Orleans Street, Baltimore, Maryland 21231, USA
    Nat Genet 36:417-22. 2004
    ....
  55. ncbi Human embryonic stem cell genes OCT4, NANOG, STELLAR, and GDF3 are expressed in both seminoma and breast carcinoma
    Uche I Ezeh
    Program in Human Embryonic Stem Cell Biology, Center for Reproductive Sciences, Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143 0556, USA
    Cancer 104:2255-65. 2005
    ..The current study was designed to identify whether expression of these 12p genes were elevated in seminoma relative to normal testis, and to determine whether elevated expression was unique to seminoma...
  56. ncbi Role of miR-143 targeting KRAS in colorectal tumorigenesis
    X Chen
    Jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, China
    Oncogene 28:1385-92. 2009
    ..Taken together, the present study provides the first evidences that miR-143 is significant in suppressing colorectal cancer cell growth through inhibition of KRAS translation...
  57. ncbi Q-Gene: processing quantitative real-time RT-PCR data
    Perikles Simon
    Section for Neurobiology of the Eye, University Eye Hospital Tuebingen, Calwerstr 7 1, 72076 Tuebingen, Germany
    Bioinformatics 19:1439-40. 2003
    ..The two procedures, which are currently in widespread use and regarded as more or less equivalent alternatives, should therefore specifically be applied according to the quantification procedure used...
  58. pmc Generation of T follicular helper cells is mediated by interleukin-21 but independent of T helper 1, 2, or 17 cell lineages
    Roza I Nurieva
    Department of Immunology, University of Texas MD Anderson Cancer Center and Graduate School of Biomedical Sciences, Houston, TX 77030, USA
    Immunity 29:138-49. 2008
    ..This study thus demonstrates that Tfh is a distinct Th cell lineage...
  59. ncbi MALAT-1, a novel noncoding RNA, and thymosin beta4 predict metastasis and survival in early-stage non-small cell lung cancer
    Ping Ji
    Department of Medicine, University of Munster, Germany
    Oncogene 22:8031-41. 2003
    ..Taken together, these data contribute to the identification of early-stage NSCLC patients that are at high risk to develop metastasis. The identification of MALAT-1 emphasizes the potential role of noncoding RNAs in human cancer...
  60. pmc T helper 17 lineage differentiation is programmed by orphan nuclear receptors ROR alpha and ROR gamma
    Xuexian O Yang
    Department of Immunology, M D Anderson Cancer Center, Houston, TX 77030, USA
    Immunity 28:29-39. 2008
    ..Therefore, Th17 differentiation is directed by two lineage-specific nuclear receptors, ROR alpha and ROR gamma...
  61. ncbi Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references
    Stéphanie Guénin
    Molecular Biology Centre, Centre de Ressources Régionales en Biologie Moléculaire, Universite de Picardie Jules Verne, Faculte des Sciences, 33 rue St Leu, F 80039 Amiens, France
    J Exp Bot 60:487-93. 2009
    ....
  62. pmc Receptor specificity of the fibroblast growth factor family. The complete mammalian FGF family
    Xiuqin Zhang
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 281:15694-700. 2006
    ..This study completes the mitogenesis-based comparison of receptor specificity of the entire FGF family under standard conditions and should help in interpreting and predicting in vivo biological activity...
  63. ncbi Facile means for quantifying microRNA expression by real-time PCR
    Rui Shi
    North Carolina State University, Raleigh, NC 27695 7247, USA
    Biotechniques 39:519-25. 2005
    ..This method also revealed miRNA tissue-specific expression patterns that cannot be resolved by Northern blot analysis and may therefore be widely useful for characterizing miRNA expression in plants as well as in animals...
  64. pmc Oct-4 expression maintained cancer stem-like properties in lung cancer-derived CD133-positive cells
    Yu Chih Chen
    Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    PLoS ONE 3:e2637. 2008
    ..Future research is warranted regarding the up-regulated expression of Oct-4 in LC-CD133(+) and malignant lung cancer...
  65. ncbi Diagnostic and prognostic microRNAs in stage II colon cancer
    Troels Schepeler
    Department of Clinical Biochemistry, Molecular Diagnostic Laboratory, Aarhus University Hospital, Aarhus N, Denmark
    Cancer Res 68:6416-24. 2008
    ....
  66. pmc Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR
    Ruibo Hu
    Institute of Crop Science, National Key Facility of Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China
    BMC Mol Biol 10:93. 2009
    ..Thus, a systematic validation of reference genes is required to ensure proper normalization. For soybean, only a small number of validated reference genes are available to date...
  67. ncbi Respiratory syncytial virus infection in elderly and high-risk adults
    Ann R Falsey
    Department of Medicine, Rochester General Hospital, Rochester, NY 14621, USA
    N Engl J Med 352:1749-59. 2005
    ..Data on the epidemiology and clinical effects in community-dwelling elderly persons and high-risk adults can help in assessing the need for vaccine development...
  68. pmc An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development
    Karen E Reid
    Faculty of Land and Food Systems, University of British Columbia, Vancouver, Canada
    BMC Plant Biol 6:27. 2006
    ..Few statistically validated reference genes have been reported in grapevine. Moreover, success in isolating high quality RNA from grapevine tissues is typically limiting due to low pH, and high polyphenolic and polysaccharide contents...
  69. pmc Selection of reference genes for quantitative gene expression normalization in flax (Linum usitatissimum L.)
    Rudy Huis
    UMR INRA USTL 1281 Stress Abiotiques et Différenciation des Végétaux Cultivés, Universite Lille 1, Villeneuve d Ascq, France
    BMC Plant Biol 10:71. 2010
    ..In this paper we evaluate different candidate HKGs for developmental transcriptomic studies in the economically-important flax fiber- and oil-crop (Linum usitatissimum L)...
  70. ncbi Plasma miR-208 as a biomarker of myocardial injury
    Xu Ji
    Department of Epidemiology, Research Institute, National Cardiovascular Center, Osaka 565 8565, Japan
    Clin Chem 55:1944-9. 2009
    ..We assessed the hypothesis that miRNAs may leak into the circulating blood from injured cells and thereby serve as biomarkers for identifying the injured cell type...
  71. ncbi Gene expression and benefit of chemotherapy in women with node-negative, estrogen receptor-positive breast cancer
    Soonmyung Paik
    Division of Pathology, Operations Center, and Biostatistical Center, National Surgical Adjuvant Breast and Bowel Project, Pittsburgh, PA 15212, USA
    J Clin Oncol 24:3726-34. 2006
    ..The relationship between the RS and chemotherapy benefit is not known...
  72. ncbi Increased expression of genes converting adrenal androgens to testosterone in androgen-independent prostate cancer
    Michael Stanbrough
    Cancer Biology Program, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Cancer Res 66:2815-25. 2006
    ..These results indicate that enhanced intracellular conversion of adrenal androgens to testosterone and dihydrotestosterone is a mechanism by which prostate cancer cells adapt to androgen deprivation and suggest new therapeutic targets...
  73. pmc Selected Toll-like receptor agonist combinations synergistically trigger a T helper type 1-polarizing program in dendritic cells
    Giorgio Napolitani
    Institute for Research in Biomedicine, Oncology Institute of Southern Switzerland, CH 6500, Bellinzona, Switzerland
    Nat Immunol 6:769-76. 2005
    ..These results identify a 'combinatorial code' by which DCs discriminate pathogens and suggest new strategies for promoting T helper type 1 responses...
  74. ncbi An overview of real-time quantitative PCR: applications to quantify cytokine gene expression
    A Giulietti
    Laboratory for Experimental Medicine and Endocrinology (LEGENDO, Catholic University of Leuven, Herestraat 49, 3000 Leuven, Belgium
    Methods 25:386-401. 2001
    ....
  75. pmc Ink4a/Arf expression is a biomarker of aging
    Janakiraman Krishnamurthy
    Department of Medicine, The Lineberger Comprehensive Cancer Center, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, 27599 7295, USA
    J Clin Invest 114:1299-307. 2004
    ..These data suggest that expression of the Ink4a/Arf tumor suppressor locus is a robust biomarker, and possible effector, of mammalian aging...
  76. pmc Maintenance of intratumoral androgens in metastatic prostate cancer: a mechanism for castration-resistant tumor growth
    R Bruce Montgomery
    Department of Medicine, University of Washington School of Medicine, Weattle, WA, USA
    Cancer Res 68:4447-54. 2008
    ..Maximal therapeutic efficacy in the treatment of castration-resistant prostate cancer will require novel agents capable of inhibiting intracrine steroidogenic pathways within the prostate tumor microenvironment...
  77. ncbi Gene silencing in cancer by histone H3 lysine 27 trimethylation independent of promoter DNA methylation
    Yutaka Kondo
    Department of Leukemia, University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA
    Nat Genet 40:741-50. 2008
    ..These data establish EZH2-mediated H3K27triM as a mechanism of tumor-suppressor gene silencing in cancer that is potentially independent of promoter DNA methylation...
  78. ncbi Alternative macrophage activation is essential for survival during schistosomiasis and downmodulates T helper 1 responses and immunopathology
    De Broski R Herbert
    University of Cape Town, Health Sciences Faculty, 7925 Cape Town, South Africa
    Immunity 20:623-35. 2004
    ..Together, this shows that alternative macrophages are essential during schistosomiasis for protection against organ injury through downregulation of egg-induced inflammation...
  79. pmc Increased expression of interleukin 17 in inflammatory bowel disease
    S Fujino
    Department of Internal Medicine, Shiga University of Medical Science, Seta Tukinowa, Otsu 520 2192, Japan
    Gut 52:65-70. 2003
    ..Interleukin (IL) 17 is a cytokine which exerts strong proinflammatory activities. In this study we evaluated changes in IL-17 expression in the inflamed mucosa and in the serum of patients with inflammatory bowel disease (IBD)...
  80. pmc Identification of somatically acquired rearrangements in cancer using genome-wide massively parallel paired-end sequencing
    Peter J Campbell
    Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK
    Nat Genet 40:722-9. 2008
    ..The results demonstrate the feasibility of systematic, genome-wide characterization of rearrangements in complex human cancer genomes, raising the prospect of a new harvest of genes associated with cancer using this strategy...
  81. ncbi Identification of novel microRNA targets based on microRNA signatures in bladder cancer
    Takahiro Ichimi
    Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan
    Int J Cancer 125:345-52. 2009
    ....
  82. pmc Local restoration of dystrophin expression with the morpholino oligomer AVI-4658 in Duchenne muscular dystrophy: a single-blind, placebo-controlled, dose-escalation, proof-of-concept study
    Maria Kinali
    The Dubowitz Neuromuscular Centre, University College London Institute of Child Health London, London, UK
    Lancet Neurol 8:918-28. 2009
    ....
  83. ncbi miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells
    Lin Xia
    State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi an, People s Republic of China
    Int J Cancer 123:372-9. 2008
    ..Taken together, our findings suggest that miR-15b and miR-16 could play a role in the development of MDR in gastric cancer cells at least in part by modulation of apoptosis via targeting BCL2...
  84. ncbi let-7 microRNA functions as a potential growth suppressor in human colon cancer cells
    Yukihiro Akao
    Department of Genetic Diagnosis, Gifu International Institute of Biotechnology, Kakamigahara, Japan
    Biol Pharm Bull 29:903-6. 2006
    ..These findings suggest the involvement of let-7 miRNA in the growth of colon cancer cells. Thus, miRNAs might provide a basis for novel RNA anti-cancer agents...
  85. pmc New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency virus type 1 RNA in plasma
    Sarah Palmer
    HIV Drug Resistance Program, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    J Clin Microbiol 41:4531-6. 2003
    ....
  86. pmc A multidrug resistance transporter from human MCF-7 breast cancer cells
    L A Doyle
    Greenebaum Cancer Center of the University of Maryland, Baltimore MD 21201, USA
    Proc Natl Acad Sci U S A 95:15665-70. 1998
    ..BCRP is a xenobiotic transporter that appears to play a major role in the multidrug resistance phenotype of MCF-7/AdrVp human breast cancer cells...
  87. ncbi Maintenance of the hematopoietic stem cell pool by CXCL12-CXCR4 chemokine signaling in bone marrow stromal cell niches
    Tatsuki Sugiyama
    Department of Medical Systems Control, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Immunity 25:977-88. 2006
    ..CXCL12-CXCR4 signaling plays an essential role in maintaining the quiescent HSC pool, and CAR cells appear to be a key component of HSC niches, including both vascular and endosteal niches in adult bone marrow...
  88. ncbi A single p450 allele associated with insecticide resistance in Drosophila
    P J Daborn
    Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Science 297:2253-6. 2002
    ..This allele is characterized by the insertion of an Accord transposable element into the 5' end of the Cyp6g1 gene...
  89. ncbi Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism
    Vincenzo Bonifati
    Genetic Epidemiologic Unit, Department of Clinical Genetics, Department of Epidemiology and Biostatistics, Erasmus Medical Center Rotterdam, Post Office Box 1738, 3000 DR Rotterdam, Netherlands
    Science 299:256-9. 2003
    ..Elucidating the physiological role of DJ-1 protein may promote understanding of the mechanisms of brain neuronal maintenance and pathogenesis of Parkinson's disease...
  90. ncbi alpha-Synuclein locus triplication causes Parkinson's disease
    A B Singleton
    Laboratory of Neurogenetics, National Institute on Aging, Bethesda, MD 20892, USA
    Science 302:841. 2003
  91. ncbi Molecular analysis of commensal host-microbial relationships in the intestine
    L V Hooper
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110, USA
    Science 291:881-4. 2001
    ..These findings provide perspectives about the essential nature of the interactions between resident microorganisms and their hosts...
  92. pmc Epigenetic differences arise during the lifetime of monozygotic twins
    Mario F Fraga
    Epigenetics Laboratory, Spanish National Cancer Centre CNIO, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    Proc Natl Acad Sci U S A 102:10604-9. 2005
    ..These findings indicate how an appreciation of epigenetics is missing from our understanding of how different phenotypes can be originated from the same genotype...
  93. pmc CD44+ CD24(-) prostate cells are early cancer progenitor/stem cells that provide a model for patients with poor prognosis
    E M Hurt
    Cancer Stem Cell Section, Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702, USA
    Br J Cancer 98:756-65. 2008
    ....
  94. ncbi Diagnostic assay based on hsa-miR-205 expression distinguishes squamous from nonsquamous non-small-cell lung carcinoma
    Danit Lebanony
    Rosetta Genomics Ltd, 10 Plaut St, Rehovot 76706, Israel
    J Clin Oncol 27:2030-7. 2009
    ..In this study, we set out to identify specific microRNA biomarkers for the identification of squamous cell carcinoma, and to use such markers for the development of a standardized assay...
  95. ncbi MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation
    Yvonne Tay
    Stem Cell and Developmental Biology, Genome Institute of Singapore, Agency for Science Technology and Research, 08 01, Genome, 60 Biopolis Street, Singapore 138672, Singapore
    Nature 455:1124-8. 2008
    ....
  96. pmc Intensive care adult patients with severe respiratory failure caused by Influenza A (H1N1)v in Spain
    Jordi Rello
    Critical Care Department, Joan XXIII University Hospital, CIBERes Enfermedades Respiratorias, IISPV, Mallafre Guasch 4, 43007 Tarragona, Spain
    Crit Care 13:R148. 2009
    ..We describe the clinical and epidemiologic characteristics of the first 32 persons reported to be admitted to the intensive care unit (ICU) due to influenza A (H1N1)v infection in Spain...
  97. pmc Rapid Akt activation by nicotine and a tobacco carcinogen modulates the phenotype of normal human airway epithelial cells
    Kip A West
    Cancer Therapeutics Branch, Cell and Cancer Biology Branch, National Cancer Institute, Bethesda, Maryland 20889, USA
    J Clin Invest 111:81-90. 2003
    ..Redundant Akt activation by nicotine and NNK could contribute to tobacco-related carcinogenesis by regulating two processes critical for tumorigenesis, cell growth and apoptosis...
  98. ncbi VSX1: a gene for posterior polymorphous dystrophy and keratoconus
    Elise Heon
    Cellular and Molecular Division, Toronto Western Research Institute, Toronto Western Hospital, 399 Bathurst Street, Toronto, Ontario, Canada M5T 2S8
    Hum Mol Genet 11:1029-36. 2002
    ..These data define the molecular basis of two important corneal dystrophies and reveal the importance of the CVC domain in the human retina...
  99. pmc Regulation of NF-kappaB inhibitor IkappaBalpha and viral replication by a KSHV microRNA
    Xiufen Lei
    Tumor Virology Program, Greehey Children s Cancer Research Institute, and Cancer Therapy and Research Center, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA
    Nat Cell Biol 12:193-9. 2010
    ..These results demonstrate an important role for KSHV miRNAs in regulating viral latency and lytic replication by manipulating the host survival pathway...
  100. ncbi Pluripotency of spermatogonial stem cells from adult mouse testis
    Kaomei Guan
    Department of Cardiology and Pneumology, Heart Center, Georg August University of Gottingen, Robert Koch Str 40, 37075 Gottingen, Germany
    Nature 440:1199-203. 2006
    ..Furthermore, these cells may provide new opportunities to study genetic diseases in various cell lineages...
  101. ncbi Viral load in patients infected with pandemic H1N1 2009 influenza A virus
    Kelvin K W To
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region, China
    J Med Virol 82:1-7. 2010
    ..Younger age was associated with prolonged shedding in the respiratory tract and higher viral load in the stool. Data from this quantitative analysis of viral shedding may have implications for formulating infection control measures...

Research Grants63

  1. Gamma-Synuclein-Mediated Regulation of Norepinephrine Transporter
    Anita Sidhu; Fiscal Year: 2010
    ..This grant is also relevant for the ARRA in that it will immediately create 3 new professional jobs. ..
  2. The role of Fli1 in T cell function and the pathogenesis of lupus
    Tamara K Nowling; Fiscal Year: 2011
    ..Additionally, insight will be gained into the regulation of T cell function in both health and disease with relevance to an array of immune and neoplastic diseases. ..
  3. Ethanol Regulation of Serum Glucocorticoid Kinase I
    BLAIR N COSTIN; Fiscal Year: 2012
    ..The work proposed here could shed light on molecular mechanisms underlying alcoholism and eventually have implications in the development of novel pharmacological agents for alcoholism or alcohol-related neurological disorders. ..
  4. Mechanisms of Early Growth Response Factor 1 (Egr-1) Induction by HSV-1 Lytic Inf
    SHAOCHUNG VICTOR HSIA; Fiscal Year: 2010
    ..b>Reverse transcriptase polymerase chain reaction (RT-PCR) analyses indicated that Egr-1 mRNA was transcribed as early as 1 hpi and did not ..
  5. Chronic Granulomatous Lung Inflammation Elicited by Carbon Nanotubes
    Mary Jane Thomassen; Fiscal Year: 2013
    ..In summary, this unique investigation of MWCNT-elicited granulomatous lung disease is well suited for student participation in an area with environmental impact on human disease. ..
  6. Development of a novel method for inhibiting diabetic retinopathy
    David R Clemmons; Fiscal Year: 2012
    ..The work in this proposal will determine if this approach is feasible using animal models of this disease state. ..
  7. Metabolic Pathways and Defects in Fructose Metabolism
    DEAN TOLAN; Fiscal Year: 2009
    ..Lastly, blood samples from African-American and Hispanic-American HFI subjects will be used for identification of gene defects by direct DNA sequencing, thus offering a reliable non-invasive diagnostic method to these Americans. ..
  8. Coronavirus modulation of cellular microRNAs in the liver: role in hepatitis
    Sonia Navas-Martin; Fiscal Year: 2012
    ..abstract_text> ..
  9. Genome-Wide Analysis of RXR-alpha Binding In Mouse Liver Chromatin with ChIP-SEQ
    SAUL JOSEPH KARPEN; Fiscal Year: 2010
    ..Such knowledge is expected to rapidly accelerate rationally-designed and validated drug discovery for liver disease. ..
  10. Neuroplasticity with alcohol dependence
    Scott C Steffensen; Fiscal Year: 2013
    ....
  11. Antiviral activity of leflunomide against respiratory syncytial virus
    WILLIAM WALDMAN; Fiscal Year: 2009
    ..Furthermore, elucidation of the molecular antiviral mechanisms of this agent may identify novel targets for future antiviral drug development. ..
  12. EGFRvIII in Pancreatic Cancer
    Maneesh Jain; Fiscal Year: 2010
    ....
  13. Receptor/Ligand Signals for Ovarian Follicular Angiogenesis
    Leon J Spicer; Fiscal Year: 2010
    ....
  14. Expression-Based Multi-Gene Signatures for CRC Recurrence and Chemoselection
    Christopher Sears; Fiscal Year: 2012
    ..developer of SmartChip[unreadable] Real-Time PCR System which will be used for improved initial biomarker discovery. ..
  15. The Role of SaeR/S During Staphylococcus aureus Skin Infections
    Jovanka M Voyich; Fiscal Year: 2012
    ..aureus pathogenesis in order to develop better methods to control/treat CA-MRSA disease and thus improve public health. ..
  16. Annual Estimates of Influenza Vaccine Effectiveness: Davidson County, TN
    Marie R Griffin; Fiscal Year: 2010
    ....
  17. Core_plus Option_A_C_Influenza Surveillance and Vaccine Effectiveness in a Large
    Richard K Zimmerman; Fiscal Year: 2013
    ..abstract_text> ..
  18. Mixed NOP/mu compounds and the involvement of their receptors in analgesia.
    LAWRENCE R TOLL; Fiscal Year: 2013
    ....
  19. Regulation of lytic and latent infection by HSV-1 encoded miRNAs
    David C Bloom; Fiscal Year: 2013
    ....
  20. Direct and Indirect Protection by Influenza Vaccine Given to Children in India
    Wayne M Sullender; Fiscal Year: 2010
    ..A major subset of FARIs will undergo realtime reverse transcriptase polymerase chain reaction testing for influenza, positive samples will be subjected to culture for virus isolation...
  21. Toll-like Receptor 4 Signaling in Adipose Tissue Macrophage Polarization
    Jeb Orr; Fiscal Year: 2012
    ....
  22. Genomics, Biomarkers and Mechanisms of Healing in Chronic Wounds
    VICTORIA KATE SHANMUGAM; Fiscal Year: 2013
    ..We propose: a. Correlating patient-reported pain scores with eicosanoid signalling pathways. b. Investigating possible relationships between exposure to different classes of analgesics, cumulative analgesic doses and wound outcome. ..
  23. Chronic Nicotine Effects on Receptor Subtypes
    Norman H Lee; Fiscal Year: 2013
    ..4. Determine the persistent indirect effects of prenatal nicotine exposure by determining how prenatal nicotine exposure alters the ability of the adolescent to respond to a new nicotine exposure ..
  24. Zinc and Alcohol-Induced Oxidative Liver Injury
    Zhanxiang Zhou; Fiscal Year: 2009
    ..These studies will provide fundamental understanding of the role of zinc in the pathophysiology of ALD, and pave the way to novel therapeutical approaches to prevention and treatment of this disease. ..
  25. A Study of ILV-94 (Anti-22 Antibody) Administered via IV in Atopic Dermatitis
    Emma Guttman; Fiscal Year: 2013
    ..IL-22 is a potential key cytokine in AD and inhibiting it may provide significant advantages over other available treatments, including: a selective target and potential safety advantages compared to broad immune suppressants. ..
  26. Ading color to cancer, adenovirus and flow cytometry to identify and capture CTCs
    CLODAGH O'SHEA; Fiscal Year: 2012
    ..abstract_text> ..
  27. Plasmacytoid Dendritic Cells and Their Role in Transplant Tolerance
    Audrey H Lau; Fiscal Year: 2013
    ..Utilizing flow cytometry, reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, mixed leukocyte reaction and T cell suppression assays, ..
  28. Molecular identification and functional characterization of fried, a gene require
    Jason Z Morris; Fiscal Year: 2010
    ..We propose to identify which gene is disrupted in fried mutants and to determine the function of fried in wild type Drosophila. ..
  29. BIN1 is a mediator and marker of cardiac reserve in heart failure.
    Tingting Hong; Fiscal Year: 2013
    ..abstract_text> ..
  30. BIN1 is a mediator and marker of cardiac reserve in heart failure.
    Tingting Hong; Fiscal Year: 2013
    ....
  31. Alterations of the Synaptic Transcriptome in Response to Repeated Ethanol
    MEGAN ANNE O'BRIEN; Fiscal Year: 2012
    ..abstract_text> ..
  32. Mesodissection Device
    Nils B Adey; Fiscal Year: 2012
    ....
  33. Differentiating Tumor Classes by Analysis of Alternative Splice Variant Patterns
    MARTHA ALLEN ZEIGER; Fiscal Year: 2010
    ..Indeed, potential application of this assay could dramatically improve the clinical management of these patients. ..
  34. Emerging Infections Program (EIP)/PPACA: Enhancing Epidemiology and Laboratory Ca
    MELISSA TOBIN-D'ANGELO; Fiscal Year: 2011
    ..Project activities include: 1) improving capacity for real- time reverse transcriptase polymerase chain reaction (rRT-PCR) influenza testing at the Georgia Public Health Laboratory;2) conducting active ..
  35. Neuronal Plasticity Related to TMD and Fibromyalgia
    Dean Dessem; Fiscal Year: 2006
    ..levels ofCGRP, SP and gene expression for CGRP, SP within the TG using radioimmunoassay and reverse transcriptase polymerase chain reaction. Hypothesis 2) SP and CGRP alter the functional properties of TG muscle afferent neurons in ..
  36. Developing an Adenoviral Vector Platform for Selective Gene Delivery to Neurons
    Travis B Lewis; Fiscal Year: 2012
    ..The availability of a delivery platform that can accommodate current and future treatment strategies will speed the translation of novel therapies from the laboratory to the clinical bedside. ..
  37. Effect of Antigen Exposure on T cell Quantity and Quality in Mucosal Tissues
    Kathryn A Fraser; Fiscal Year: 2010
    ..This information is extremely necessary for the future development of a HIV vaccine that targets the cellular immune system. ..
  38. Photic and Circadian Regulation of Retinal Melatonin
    Gianluca Tosini; Fiscal Year: 2011
    ..In our research, we will use a wide array of new and technologically advanced techniques, such as quantitative real time Q-RTPCR, laser capture dissecting microscopy, and microarray analysis. ..
  39. SOMATOSTATIN RECEPTORS--CLASSIFICATION OF NEUROBLASTOMAS
    M O DORISIO; Fiscal Year: 1999
    ..We anticipate this basic research will ultimately translate into a molecular classification of neuroblastoma and used to design more effective therapies for this pediatric tumor. ..
  40. GENETICS OF H. AEGYPTIUS BRAZILIAN PURPURIC FEVER CLONE
    Luis Actis; Fiscal Year: 2000
    ..aegyptius. Furthermore, these studies will lead to a better comprehension of the nature of other vascular destructive infectious diseases. ..
  41. RETROVIRAL ETIOLOGY OF PRIMARY BILIARY CIRRHOSIS
    Andrew Mason; Fiscal Year: 2002
    ....
  42. Mechanisms of HAART-Induced Endothelial Dysfunction
    Krishna Agrawal; Fiscal Year: 2006
    ..These studies should provide evidence in support of our hypotheses that disruption of NO production by HAART results in endothelial dysfunction, which is further exacerbated in the presence of TNFalpha and/or Tat protein. ..
  43. CALCITONIN RECEPTOR GENE EXPRESSION
    Steven Goldring; Fiscal Year: 2002
    ..g. kidney). This will define the structural basis of CTR gene regulation and permit identification of potentially unique factors that regulate the CTR. ..
  44. NEUROTROPHIN ROLE IN OXIDATIVE HOMEOSTASIS AFTER STROKE
    JOSE PEREZ POLO; Fiscal Year: 1999
    ..spectrophotometrically and the mRNAs by Northern analysis, RNA protection assays, and reverse transcriptase polymerase chain reaction, with suitable controls...
  45. Genetic Markers of Acute Pancreas Allograft Rejection
    Ann Cashion; Fiscal Year: 2002
    ..perform, granzyme B, and Fas ligand, using a newly developed molecular technique (real-time reverse transcriptase polymerase chain reaction [RT-PCR]), we can noninvasively detect acute allograft rejection at the time or prior to ..
  46. Voltage-gated calcium channels in taste buds
    Nirupa Chaudhari; Fiscal Year: 2003
    ..Through these aims, we hope to gain a novel perspective on voltage-gated calcium channels, which play critical roles in all neuronal systems, but have been minimally studied in taste cells to date. ..
  47. Targeting Egr-1 with Curcumin Analogs for Prostate Cancer Prevention
    Marco Bisoffi; Fiscal Year: 2009
    ..prostatic tissues through gene array analysis, and confirmed our initial observation by reverse transcriptase polymerase chain reaction. We hypothesize that Egr-1 expression is not only indicative of prostatic field cancerization,..
  48. ANOPHELES STEPHENSI NO SYNTHASE--IMPACT ON PLASMODIUM
    Shirley Luckhart; Fiscal Year: 2001
    ....
  49. OPIATE PRECURSOR PROCESSING
    Vivian Hook; Fiscal Year: 1999
    ..Overall these studies will establish the relative importance of PTP, compared to PC 1/3 and PC2, in PE processing. ..
  50. Deciphering the Olfactory Code
    Albert Folch; Fiscal Year: 2005
    ..abstract_text> ..
  51. FGF SIGNALING AND PITUITARY TUMORIGENESIS
    ANDREW BRADFORD; Fiscal Year: 2001
    ....
  52. REGULATION OF SERINE HYDROXYMETHYLTRANSFERASE
    PATRICK STOVER; Fiscal Year: 1999
    ....
  53. MOLECULAR REGUL OF HUMAN COLON CANCER LIVER METASTASIS
    Robert Radinsky; Fiscal Year: 1999
    ....
  54. GRP AND ITS RECEPTORS: ROLE IN GASTRIC CANCER
    Robert Carroll; Fiscal Year: 2003
    ..These studies will allow the applicants to determine the contribution of the GRP-R to the development and progression of gastric adenocarcinomas, and potentially will lead to therapeutic advances in the treatment of this disease. ..
  55. Activators of Adipocyte Fatty Acid Oxidation
    CYDNEY BROOKS; Fiscal Year: 2003
    ..Development of such compounds may lead to therapeutic interventions that are effective for modulating lipid metabolism and treating obesity. ..
  56. Role of Aldose Reductase-Like-1 in Colon Tumorigenesis
    Deliang Cao; Fiscal Year: 2009
    ..and function of ARL-1 gene in inflammatory and malignant colon tissues using real time reverse transcriptase polymerase chain reaction, Western blot, immunohistochemistry, and ARL-1 enzymatic activity/gene sequencing analysis...
  57. NO/CGMP RELAXATION IN PULMONARY HYPERTENSION
    Brian Fouty; Fiscal Year: 2000
    ..To determine the molecular and biophysical characteristics of the channel they plan to express the full length gene in Chinese hamster ovary cells. ..
  58. HTERT GENE EXPRESSION IN SUSPICIOUS THYROID FNA SAMPLES
    Martha Zeiger; Fiscal Year: 2001
    ..She has developed a detection method for hTERT gene expression using reverse transcriptase polymerase chain reaction (RT-PCR) and examined its expression in both thyroid nodules and FNAs...
  59. Mechanisms of Ethanol's Influence on Sleep and Circadian Rhythms
    TIFFANY HUITT; Fiscal Year: 2009
    ....
  60. SYNGENEIC GRAFT-VS-HOST DISEASE
    Allan Hess; Fiscal Year: 2000
    ..and the cytokine profile of the autoreactive T-cell subsets will be assessed in vitro by the reverse transcriptase polymerase chain reaction in clones from limiting dilution cultures...
  61. AN IN VITRO MODEL OF THE FOLLICLE ASSOCIATED EPITHELIUM
    ELEANOR METCALF; Fiscal Year: 2001
    ....
  62. Molecular Correlates of Soy in Humans
    Raymond Bergan; Fiscal Year: 2003
    ..Metastatic prostate cells can be detected by amplifying PSA specific RNA by reverse transcriptase polymerase chain reaction (RT/PCR)...
  63. MOLECULAR STAGING OF LYMPH NODES--OUTCOME OF PROSTATE CA
    Anna Ferrari; Fiscal Year: 2000
    ..abstract_text> ..