in situ hybridization

Summary

Summary: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.

Top Publications

  1. pmc Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway
    Eek Hoon Jho
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 22:1172-83. 2002
  2. ncbi Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation
    R Dasgupta
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology and Committee on Developmental Biology, The University of Chicago, Chicago, IL 60637, USA
    Development 126:4557-68. 1999
  3. doi High-resolution in situ hybridization to whole-mount zebrafish embryos
    Christine Thisse
    Department of Cell Biology, University of Virginia Health Sciences Center, PO Box 800732, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA
    Nat Protoc 3:59-69. 2008
  4. ncbi Optical projection tomography as a tool for 3D microscopy and gene expression studies
    James Sharpe
    Medical Research Council, Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK
    Science 296:541-5. 2002
  5. pmc Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors
    Silvia Maretto
    Histology and Embryology Section, Department of Histology, Microbiology, and Medical Biotechnology, University of Padua, 35131 Padua, Italy
    Proc Natl Acad Sci U S A 100:3299-304. 2003
  6. ncbi American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer
    Antonio C Wolff
    American Society of Clinical Oncology, Alexandria, VA, USA
    J Clin Oncol 25:118-45. 2007
  7. ncbi Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization
    Kala Jessie
    Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
    J Infect Dis 189:1411-8. 2004
  8. ncbi Sox9 is required for cartilage formation
    W Bi
    Department of Molecular Genetics, The University of Texas, M D Anderson Cancer Center, Houston 77030, USA
    Nat Genet 22:85-9. 1999
  9. doi Improved survival of patients with human papillomavirus-positive head and neck squamous cell carcinoma in a prospective clinical trial
    Carole Fakhry
    Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    J Natl Cancer Inst 100:261-9. 2008
  10. ncbi Sonic hedgehog mediates the polarizing activity of the ZPA
    R D Riddle
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Cell 75:1401-16. 1993

Research Grants

  1. Transcription factors regulating sensory gene expression and pain pathways
    Eric E Turner; Fiscal Year: 2011
  2. Optimization of DNA Repair by Abh8
    Lauren Endres; Fiscal Year: 2013
  3. GENETIC ANALYSIS OF POLARITY
    RUTH M STEWARD; Fiscal Year: 2010
  4. Unbiased genome-wide screen to identify genes regulating mucous cell hyperplasia
    Jeffrey A Whitsett; Fiscal Year: 2010
  5. Ghrelin: Behavioral, Anatomical, and Physiological Components of Obesity
    BRETT JOHN WARREN TEUBNER; Fiscal Year: 2011
  6. Estrogen Modulation of Auditory Processing
    Raphael Pinaud; Fiscal Year: 2012
  7. Mechanisms of Angiogenesis in Retinopathy of Prematurity
    Mary Elizabeth Hartnett; Fiscal Year: 2009
  8. NEURAL AND ENDOCRINE CONSEQUENCES OF PARITY
    ROBERT STAFFORD BRIDGES; Fiscal Year: 2012
  9. Mechanisms of Angiogenesis of Retinopathy of Prematurity
    MARY ELIZABETH RUTH HARTNETT; Fiscal Year: 2013
  10. RADIATION-INDUCED THYROID CANCER
    Heinz Ulrich G Weier; Fiscal Year: 2011

Detail Information

Publications406 found, 100 shown here

  1. pmc Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway
    Eek Hoon Jho
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 22:1172-83. 2002
    ..Our results suggest that Axin2 participates in a negative feedback loop, which could serve to limit the duration or intensity of a Wnt-initiated signal...
  2. ncbi Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation
    R Dasgupta
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology and Committee on Developmental Biology, The University of Chicago, Chicago, IL 60637, USA
    Development 126:4557-68. 1999
    ..Furthermore, our findings unveil the importance of activated LEF/TCF complexes at distinct times in hair development and cycling when changes in cell fate and differentiation commitments take place...
  3. doi High-resolution in situ hybridization to whole-mount zebrafish embryos
    Christine Thisse
    Department of Cell Biology, University of Virginia Health Sciences Center, PO Box 800732, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA
    Nat Protoc 3:59-69. 2008
    The in situ hybridization (ISH) technique allows the sites of expression of particular genes to be detected. This protocol describes ISH of digoxigenin-labeled antisense RNA probes to whole-mount zebrafish embryos...
  4. ncbi Optical projection tomography as a tool for 3D microscopy and gene expression studies
    James Sharpe
    Medical Research Council, Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK
    Science 296:541-5. 2002
    ..OPT microscopy allows the rapid mapping of the tissue distribution of RNA and protein expression in intact embryos or organ systems and can therefore be instrumental in studies of developmental biology or gene function...
  5. pmc Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors
    Silvia Maretto
    Histology and Embryology Section, Department of Histology, Microbiology, and Medical Biotechnology, University of Padua, 35131 Padua, Italy
    Proc Natl Acad Sci U S A 100:3299-304. 2003
    ..In summary, BAT-gal mice unveil the entire complexity of Wntbeta-catenin signaling in mammals and have broad application potentials for the identification of Wnt-responsive cell populations in development and disease...
  6. ncbi American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer
    Antonio C Wolff
    American Society of Clinical Oncology, Alexandria, VA, USA
    J Clin Oncol 25:118-45. 2007
    ..To develop a guideline to improve the accuracy of human epidermal growth factor receptor 2 (HER2) testing in invasive breast cancer and its utility as a predictive marker...
  7. ncbi Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization
    Kala Jessie
    Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
    J Infect Dis 189:1411-8. 2004
    ..patients with serologically or virologically confirmed dengue infections by immunohistochemistry (IHC) and in situ hybridization (ISH), to localize viral antigen and RNA, respectively...
  8. ncbi Sox9 is required for cartilage formation
    W Bi
    Department of Molecular Genetics, The University of Texas, M D Anderson Cancer Center, Houston 77030, USA
    Nat Genet 22:85-9. 1999
    ..Moreover, no cartilage developed in teratomas derived from Sox9-/- embryonic stem (ES) cells. Our results identify Sox9 as the first transcription factor that is essential for chondrocyte differentiation and cartilage formation...
  9. doi Improved survival of patients with human papillomavirus-positive head and neck squamous cell carcinoma in a prospective clinical trial
    Carole Fakhry
    Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    J Natl Cancer Inst 100:261-9. 2008
    ....
  10. ncbi Sonic hedgehog mediates the polarizing activity of the ZPA
    R D Riddle
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Cell 75:1401-16. 1993
    ..Like the ZPA, Sonic hedgehog expression leads to the activation of Hox genes. Sonic hedgehog thus appears to function as the signal for antero-posterior patterning in the limb...
  11. ncbi Expression of the T-box family genes, Tbx1-Tbx5, during early mouse development
    D L Chapman
    Department of Genetics and Development, College of Physicans and Surgeons, Columbia University, New York, New York 10032, USA
    Dev Dyn 206:379-90. 1996
    ..across a wide range of embryonic stages from blastocyst through gastrulation and early organogenesis by in situ hybridization of wholemounts and tissue sections. Tbx3 is expressed earliest, in the inner cell mass of the blastocyst...
  12. ncbi Regulation of cerebral cortical size by control of cell cycle exit in neural precursors
    Anjen Chenn
    Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
    Science 297:365-9. 2002
    ....
  13. ncbi Control of cell behavior during vertebrate development by Slug, a zinc finger gene
    M A Nieto
    Instituto Cajal, Madrid, Spain
    Science 264:835-9. 1994
    ..This change, which is the transition from epithelial to mesenchymal character, occurs at the formation of mesoderm during gastrulation and on emigration of the neutral crest from the neural tube...
  14. ncbi MicroRNA expression in zebrafish embryonic development
    Erno Wienholds
    Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, The Netherlands
    Science 309:310-1. 2005
    ....
  15. ncbi The role of Pax-6 in eye and nasal development
    J C Grindley
    Developmental Genetics Section, Western General Hospital, Edinburgh, UK
    Development 121:1433-42. 1995
    ....
  16. ncbi Dependence of stem cell fate in Arabidopsis on a feedback loop regulated by CLV3 activity
    U Brand
    Institut fur Entwicklungsbiologie, Universitat zu Koln, Gyrhofstrabetae 17, D 50923 Köln, Germany
    Science 289:617-9. 2000
    ..We also demonstrate that the CLV pathway acts by repressing the activity of the transcription factor WUSCHEL, an element of the positive, stem cell-promoting pathway...
  17. ncbi Planarian homologs of netrin and netrin receptor are required for proper regeneration of the central nervous system and the maintenance of nervous system architecture
    Francesc Cebria
    Department of Cell and Developmental Biology and Neuroscience Program, University of Illinois at Urbana Champaign, B107 Chemical and Life Sciences Laboratory, 601 South Goodwin Avenue, Urbana, IL 61801, USA
    Development 132:3691-703. 2005
    ..These results suggest a crucial role for guidance cues, not only in CNS regeneration but also in maintenance of neural architecture...
  18. pmc Correlation between MET gene copy number by silver in situ hybridization and protein expression by immunohistochemistry in non-small cell lung cancer
    Rafal Dziadziuszko
    Medical University of Gdansk, Gdansk, Poland
    J Thorac Oncol 7:340-7. 2012
    ..Therefore, a detailed understanding of relationship between these markers and prognosis is essential...
  19. ncbi Bone morphogenetic proteins (BMPs) as regulators of dorsal forebrain development
    Y Furuta
    Howard Hughes Medical Institute, Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 2175, USA
    Development 124:2203-12. 1997
    ..To investigate this problem, we have compared by in situ hybridization the expression of five Bmp genes belonging to the Drosophila Decapentaplegic (Bmp2 and Bmp4) and 60A ..
  20. ncbi Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements
    Michael T Veeman
    Department of Pharmacology, Center for Developmental Biology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA 98195, USA
    Curr Biol 13:680-5. 2003
    ..Our results support the idea that a vertebrate PCP pathway regulates gastrulation movements and suggest that there is overlap between the PCP and Wnt/calcium pathways...
  21. doi Prognostic significance of MMP-9 and TIMP-1 serum and tissue expression in breast cancer
    Zheng Sheng Wu
    Department of Pathology, Anhui Medical University, Hefei, Anhui, People s Republic of China
    Int J Cancer 122:2050-6. 2008
    ..and mRNA in paraffin-embedded tumor tissues from the 60 breast cancer patients by immunohistochemistry and in situ hybridization. We then correlated serum and tissue levels of MMP-9 and TIMP-1 in breast cancer samples and their ..
  22. ncbi The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo
    S Schulte-Merker
    Abteilung Genetik, Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    Development 116:1021-32. 1992
    ..Exposure of animal cap cells to activinA induces Zf-T expression, as does transplantation into the germ ring...
  23. pmc Formaldehyde-based whole-mount in situ hybridization method for planarians
    Bret J Pearson
    Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, Utah, USA
    Dev Dyn 238:443-50. 2009
    Whole-mount in situ hybridization (WISH) is a powerful tool for visualizing gene expression patterns in specific cell and tissue types...
  24. pmc An anatomically comprehensive atlas of the adult human brain transcriptome
    Michael J Hawrylycz
    Allen Institute for Brain Science, Seattle, Washington 98103, USA
    Nature 489:391-9. 2012
    ..This freely accessible online data resource forms a high-resolution transcriptional baseline for neurogenetic studies of normal and abnormal human brain function...
  25. ncbi Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning
    S Davis
    Regeneron Pharmaceuticals, Inc, Tarrytown, New York 10591, USA
    Cell 87:1161-9. 1996
    ..However, its expression in close proximity with developing blood vessels implicates Angiopoietin-1 in endothelial developmental processes...
  26. pmc BMP signaling is necessary for patterning the sensory and nonsensory regions of the developing mammalian cochlea
    Takahiro Ohyama
    Division of Cell Biology and Genetics, House Ear Institute, Los Angeles, California 90057, USA
    J Neurosci 30:15044-51. 2010
    ..Our results suggest BMP signaling is required for patterning sensory and nonsensory tissue in the mammalian cochlea...
  27. pmc Expression of ghrelin receptor mRNA in the rat and the mouse brain
    Jeffrey M Zigman
    Department of Medicine and Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Comp Neurol 494:528-48. 2006
    ..Its distribution in the CNS, as assessed by in situ hybridization histochemistry (ISHH), has been described previously in a few mammalian species, although these studies ..
  28. ncbi Ontogeny and behaviour of early macrophages in the zebrafish embryo
    P Herbomel
    Unité de Biologie moléculaire du Développement, URA1947 du CNRS, Departement de Biologie Moleculaire, Institut Pasteur, 75724 Paris Cedex 15, France
    Development 126:3735-45. 1999
    ....
  29. ncbi Characterization of Wnt gene expression in developing and postnatal hair follicles and identification of Wnt5a as a target of Sonic hedgehog in hair follicle morphogenesis
    S Reddy
    Departments of Dermatology and Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mech Dev 107:69-82. 2001
    ..These results identify candidates for several key follicular signals and suggest that WNT and SHH signaling pathways interact to regulate hair follicle morphogenesis...
  30. ncbi The transcription factors L-Sox5 and Sox6 are essential for cartilage formation
    P Smits
    Department of Molecular Genetics, The University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Dev Cell 1:277-90. 2001
    ..L-Sox5 and Sox6 are thus redundant, potent enhancers of chondroblast functions, thereby essential for endochondral skeleton formation...
  31. ncbi beta-Catenin is required for specification of proximal/distal cell fate during lung morphogenesis
    Michael L Mucenski
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 278:40231-8. 2003
    ..The mice, however, died at birth because of respiratory failure. Analysis of the lungs by in situ hybridization and immunohistochemistry, using molecular markers of the epithelial and mesenchymal components of both ..
  32. ncbi Expression of zebrafish goosecoid and no tail gene products in wild-type and mutant no tail embryos
    S Schulte-Merker
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Genetik, Tubingen, Germany
    Development 120:843-52. 1994
    ..Studies with mutant embryos suggest that gsc is independent of ntl function in vivo...
  33. ncbi Lithium perturbation and goosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish
    S E Stachel
    Department of Human Genetics, University of Utah, Salt Lake City 84112
    Development 117:1261-74. 1993
    ..Our results demonstrate that the zebrafish dorsal axis is signaled by a pathway initiated in the cleavage-stage embryo. Furthermore, they provide novel insights into anterior morphogenesis...
  34. ncbi Colocalization of GABA and glycine in the ventral nucleus of the lateral lemniscus in rat: an in situ hybridization and semiquantitative immunocytochemical study
    R Riquelme
    Laboratory for the Neurobiology of Hearing, Department of Cell Biology and Pathology, University of Salamanca, , E-37007 Salamanca, Spain
    J Comp Neurol 432:409-24. 2001
    We have studied by in situ hybridization for GAD65 mRNA in thick sections and by semiquantitative postembedding immunocytochemistry in consecutive semithin sections, the expression of gamma-aminobutyric acid (GABA) and glycine in cell ..
  35. ncbi An essential role for beta-actin mRNA localization and translation in Ca2+-dependent growth cone guidance
    Jiaqi Yao
    Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Nat Neurosci 9:1265-73. 2006
    ....
  36. ncbi The arterial pole of the mouse heart forms from Fgf10-expressing cells in pharyngeal mesoderm
    R G Kelly
    Departement de Biologie Moleculaire, Institut Pasteur, Paris, France
    Dev Cell 1:435-40. 2001
    ..25 and 10.5. These data suggest that arterial pole myocardium originates outside the classical heart field...
  37. ncbi Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocol
    A F Moorman
    Experimental and Molecular Cardiology Group, Academic Medical Center, Amsterdam, The Netherlands
    J Histochem Cytochem 49:1-8. 2001
    ..in situ mRNA detection in conjunction with high spatial resolution, we developed a nonradioactive in situ hybridization (ISH) protocol for detection of mRNA sequences in sections...
  38. ncbi fork head domain genes in zebrafish
    J Odenthal
    MPI für Entwicklungsbiologie, Spemannstrasse 35 III, D 72076 Tubingen, Germany
    Dev Genes Evol 208:245-58. 1998
    ..They show unique expression patterns in whole-mount RNA in situ hybridization during the first 24 h of embryonic development...
  39. pmc The maintenance and regeneration of the planarian excretory system are regulated by EGFR signaling
    Jochen C Rink
    Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84132, USA
    Development 138:3769-80. 2011
    ....
  40. ncbi Coordinate regulation of motor neuron subtype identity and pan-neuronal properties by the bHLH repressor Olig2
    B G Novitch
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Columbia University, 701 West 168th Street, New York, NY 10032, USA
    Neuron 31:773-89. 2001
    ..Together, these studies show that Olig2 has a critical role in integrating diverse features of motor neuron differentiation in the developing spinal cord...
  41. ncbi Specific localisation of human cytomegalovirus nucleic acids and proteins in human colorectal cancer
    Lualhati Harkins
    Pathology Service, Birmingham Veterans Affairs Hospital, Birmingham, AL, USA
    Lancet 360:1557-63. 2002
    ..We aimed to assess whether gene products of human cytomegalovirus could be detected in colorectal cancers...
  42. ncbi Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo
    H Sasaki
    Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee 37232
    Development 118:47-59. 1993
    ..The other two are novel and have been named MF-1 and MF-2 (for mesoderm/mesenchyme fork head). Wholemount in situ hybridization of embryos between 6.5 and 9.5 days p. c. shows that each gene has a unique expression pattern...
  43. ncbi Pet-1 ETS gene plays a critical role in 5-HT neuron development and is required for normal anxiety-like and aggressive behavior
    Timothy J Hendricks
    Department of Neurosciences, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Neuron 37:233-47. 2003
    ..These findings indicate that Pet-1 is a critical determinant of 5-HT neuron identity and implicate a Pet-1-dependent program in serotonergic modulation of behavior...
  44. doi The biology of coral metamorphosis: molecular responses of larvae to inducers of settlement and metamorphosis
    L C Grasso
    Centre for the Molecular Genetics of Development, Molecular Genetics and Evolution Group, Research School of Biological Sciences, Australian National University, P O Box 475, Canberra, ACT, 2612, Australia
    Dev Biol 353:411-9. 2011
    ..patterns of specific genes, selected on the basis of the array experiments, were investigated by in situ hybridization. Three patterns of expression were common-an aboral pattern restricted to the searching/settlement phase, ..
  45. pmc Genome-wide analyses reveal a role for peptide hormones in planarian germline development
    James J Collins
    Howard Hughes Medical Institute and Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, Urbana, Illinois, United States of America
    PLoS Biol 8:e1000509. 2010
    ..Comprehensive in situ hybridization analyses of prohormone gene expression revealed the unanticipated complexity of the flatworm nervous ..
  46. doi Organization of two independent kisspeptin systems derived from evolutionary-ancient kiss genes in the brain of zebrafish
    Arianna Servili
    Neurogenesis And Œstrogens, Unité Mixte de Recherche Centre National de la Recherche Scientifique 6026, Institut Federatif de Recherche 140, Campus de Beaulieu, 35042 Rennes Cedex, France
    Endocrinology 152:1527-40. 2011
    ..Immunohistochemistry fully confirmed in situ hybridization data, showing that kiss1-expressing neurons are only located in the habenular nucleus, while kiss2-..
  47. ncbi Argonaute2 is the catalytic engine of mammalian RNAi
    Jidong Liu
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 305:1437-41. 2004
    ..Thus, our evidence supports a model in which Argonaute contributes "Slicer" activity to RISC, providing the catalytic engine for RNAi...
  48. doi Biological and prognostic associations of miR-205 and let-7b in breast cancer revealed by in situ hybridization analysis of micro-RNA expression in arrays of archival tumour tissue
    John Le Quesne
    Cancer Research UK, Cambridge Research Institute and Department of Oncology, University of Cambridge, UK
    J Pathol 227:306-14. 2012
    ..We used locked nucleic acid probe in situ hybridization (LNA-ISH) to visualize, in tissue micro-arrays (TMAs) of 2919 formalin-fixed paraffin-embedded (FFPE) ..
  49. pmc Intrinsic circadian clock of the mammalian retina: importance for retinal processing of visual information
    Kai Florian Storch
    Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    Cell 130:730-41. 2007
    ..Our findings indicate that the intrinsic circadian clock of the retina regulates retinal visual processing in vivo...
  50. pmc Systematic image-driven analysis of the spatial Drosophila embryonic expression landscape
    Erwin Frise
    Department of Genome Dynamics, Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Mol Syst Biol 6:345. 2010
    ..The known genes suggest developmental roles for the clusters, and identification of related patterns predicts requirements for co-occurring biological functions...
  51. pmc An in situ hybridization study of the insulin-like growth factor system in developing condylar cartilage of the fetal mouse mandible
    S Shibata
    Maxillofacial Anatomy, Department of Maxillofacial Biology, Graduate School, Tokyo Medical and Dental University, Tokyo 113 8549, Japan
    Eur J Histochem 56:e23. 2012
    ..Fetal mice at embryonic day (E) 13.0-18.5 were used for in situ hybridization studies using [35S]-labeled RNA probes for IGF-I, IGF-II, IGF-I receptor (-IR), and IGF binding proteins (-..
  52. ncbi Essential role of non-canonical Wnt signalling in neural crest migration
    Jaime De Calisto
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Development 132:2587-97. 2005
    ....
  53. pmc Whole mount in situ hybridization methodology for Schistosoma mansoni
    Alexis A Cogswell
    Rush University Medical Center, Department of Immunology Microbiology, Chicago, IL, United States
    Mol Biochem Parasitol 178:46-50. 2011
    ..mansoni, limiting the usefulness of the sequence data. Here we describe a whole mount in situ hybridization (WISH) method that can be used to identify the tissue-specific expression of transcripts in S. mansoni...
  54. ncbi Mitochondrial abnormalities in Alzheimer's disease
    K Hirai
    Institute of Pathology, Department of Neurology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Neurosci 21:3017-23. 2001
    ..In this study, we used in situ hybridization to mitochondrial DNA (mtDNA), immunocytochemistry of cytochrome oxidase, and morphometry of electron ..
  55. pmc MicroRNA-21 is overexpressed in pancreatic cancer and a potential predictor of survival
    Mary Dillhoff
    Department of Surgery, The Ohio State University, 410 W 10th Ave, N924 Doan Hall, Columbus, OH 43210, USA
    J Gastrointest Surg 12:2171-6. 2008
    ..We sought to determine the expression pattern of miR-21 in pancreatic cancers and its impact on clinicopathologic characteristics...
  56. ncbi Pentylenetetrazole induced changes in zebrafish behavior, neural activity and c-fos expression
    S C Baraban
    Epilepsy Research Laboratory, Department of Neurological Surgery, University of California, San Francisco, Box 0520, 503 Parnassus Avenue, San Francisco, CA 94143, USA
    Neuroscience 131:759-68. 2005
    ..Therefore, zebrafish larvae represent a powerful new system to study the underlying basis of seizure generation, epilepsy and epileptogenesis...
  57. ncbi The Sry-related gene Sox9 is expressed during chondrogenesis in mouse embryos
    E Wright
    Centre for Molecular and Cellular Biology, University of Queensland, Brisbane, Australia
    Nat Genet 9:15-20. 1995
    ..The expression pattern and chromosomal location of Sox9 suggest that it may be the gene defective in the mouse skeletal mutant Tail-short, a potential animal model for campomelic dysplasia...
  58. doi Robust one-day in situ hybridization protocol for detection of microRNAs in paraffin samples using LNA probes
    Stine Jørgensen
    Exiqon A S, Diagnostic Product Development, Skelstedet 16, Vedbæk, Denmark
    Methods 52:375-81. 2010
    ..experimental formalin-fixed and paraffin-embedded samples we have developed a simple and robust one-day in situ hybridization protocol based on the use of double digoxigenin (DIG)-labeled LNA-DNA chimeric probes...
  59. ncbi In situ hybridization analysis of anterior pituitary hormone gene expression during fetal mouse development
    M A Japón
    Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland
    J Histochem Cytochem 42:1117-25. 1994
    ....
  60. ncbi Retinoic acid controls the bilateral symmetry of somite formation in the mouse embryo
    Julien Vermot
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP College de France, BP 10142, 67404 Illkirch Cedex, Strasbourg, France
    Science 308:563-6. 2005
    ..These data implicate retinoic acid as an endogenous signal that maintains the bilateral synchrony of mesoderm segmentation, and therefore controls bilateral symmetry, in vertebrate embryos...
  61. ncbi Math1 is expressed in temporally discrete pools of cerebellar rhombic-lip neural progenitors
    Rob Machold
    Department of Cell Biology, The Skirball Institute of Biomolecular Medicine, New York University Medical Center, 540 First Avenue, New York, New York 10016, USA
    Neuron 48:17-24. 2005
    ..Thus, we demonstrate that the cerebellar rhombic lip gives rise to multiple cell types within rhombomere 1...
  62. ncbi Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility
    H L Zhang
    Department of Neuroscience, Rose F. Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Neuron 31:261-75. 2001
    ..Growth cones exhibited impaired motility in the presense of antisense. These results suggest a novel mechanism to influence growth cone dynamics involving the regulated transport of mRNA...
  63. pmc GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo
    E E Morrisey
    Department of Medicine, University of Chicago, Chicago, Illinois 60637 USA
    Genes Dev 12:3579-90. 1998
    ..In addition, these data demonstrate that GATA6 is required for establishment of the endodermally derived bronchial epithelium...
  64. ncbi Altered MicroRNA expression confined to specific epithelial cell subpopulations in breast cancer
    Lorenzo F Sempere
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire, USA
    Cancer Res 67:11612-20. 2007
    ..To obtain insight into miRNA deregulation in breast cancer, we implemented an in situ hybridization (ISH) method to reveal the spatial distribution of miRNA expression in archived formalin-fixed, paraffin-..
  65. pmc Disruption of Fgf10/Fgfr2b-coordinated epithelial-mesenchymal interactions causes cleft palate
    Ritva Rice
    Departments of Craniofacial Development and Orthodontics, King s College, London, United Kingdom
    J Clin Invest 113:1692-700. 2004
    ..We show that coordinated epithelial-mesenchymal interactions are essential during the initial stages of palate development and require an Fgf-Shh signaling network...
  66. ncbi Wnt-4 is a mesenchymal signal for epithelial transformation of metanephric mesenchyme in the developing kidney
    A Kispert
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Development 125:4225-34. 1998
    ....
  67. ncbi Neural crest cell-cell adhesion controlled by sequential and subpopulation-specific expression of novel cadherins
    S Nakagawa
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Development 121:1321-32. 1995
    ..The cadherin-mediated specific interaction between crest cells likely plays a role in intercellular signaling between homotypic cells as well as in sorting of heterotypic cells...
  68. ncbi Animal MicroRNAs confer robustness to gene expression and have a significant impact on 3'UTR evolution
    Alexander Stark
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell 123:1133-46. 2005
    ..This mutually exclusive expression argues that microRNAs confer accuracy to developmental gene-expression programs, thus ensuring tissue identity and supporting cell-lineage decisions...
  69. ncbi Visualizing an olfactory sensory map
    P Mombaerts
    Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute and Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA
    Cell 87:675-86. 1996
    ..This genetic approach may be more broadly applied to visualize the development and plasticity of projections in the mammalian nervous system...
  70. pmc miRNA in situ hybridization in formaldehyde and EDC-fixed tissues
    John T G Pena
    Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10065, USA
    Nat Methods 6:139-41. 2009
    MicroRNAs are small regulatory RNAs with many biological functions and disease associations. We showed that in situ hybridization (ISH) using conventional formaldehyde fixation results in substantial microRNA loss from mouse tissue ..
  71. ncbi MCP-1, MCP-2 and MCP-3 expression in multiple sclerosis lesions: an immunohistochemical and in situ hybridization study
    C McManus
    Department of Pathology Neuropathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Neuroimmunol 86:20-9. 1998
    ..MS) lesions of differing ages and levels of inflammatory activity using immunohistochemistry and in situ hybridization. In acute and chronic-active MS lesions immunoreactivity for MCP-1, -2 and -3 was prominent throughout the ..
  72. ncbi Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse
    Nobuaki Tamamaki
    Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Comp Neurol 467:60-79. 2003
    ..The distribution pattern of GFP-positive somata was the same as that of the GAD67 in situ hybridization signal in the central nervous system...
  73. pmc A wound-induced Wnt expression program controls planarian regeneration polarity
    Christian P Petersen
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 106:17061-6. 2009
    ..These data indicate that wound-induced input is involved in resetting the normal polarized features of the body axis during regeneration...
  74. ncbi Sox2 deficiency causes neurodegeneration and impaired neurogenesis in the adult mouse brain
    Anna L M Ferri
    Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy
    Development 131:3805-19. 2004
    ..These findings highlight a crucial and unexpected role for Sox2 in the maintenance of neurones in selected brain areas, and suggest a contribution of neural cell proliferative defects to the pathological phenotype...
  75. ncbi Wnt-11 activation of a non-canonical Wnt signalling pathway is required for cardiogenesis
    Petra Pandur
    1 Abt Entwicklungsbiochemie, Junior Group SFB 271, Universitat Gottingen, Humboldtallee 23, D 37073 Gottingen, Germany 2 Abt Biochemie, Universitat Ulm, Albert Einstein Allee 11, D 89081 Ulm, Germany
    Nature 418:636-41. 2002
    ..Our results indicate that the cardiac developmental program requires non-canonical Wnt signal transduction...
  76. doi Transcriptionally-active high-risk human papillomavirus is rare in oral cavity and laryngeal/hypopharyngeal squamous cell carcinomas--a tissue microarray study utilizing E6/E7 mRNA in situ hybridization
    James S Lewis
    Department of Pathology and Immunology, Washington University, St Louis, MO 63110, USA
    Histopathology 60:982-91. 2012
    ..In particular, the significance of finding viral DNA is not known. We sought to evaluate nonoropharyngeal squamous cell carcinomas for transcriptionally-active human papillomavirus and to compare this with the presence of viral DNA...
  77. ncbi Math1 expression redefines the rhombic lip derivatives and reveals novel lineages within the brainstem and cerebellum
    Vincent Y Wang
    Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Neuron 48:31-43. 2005
    ....
  78. pmc The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 102:10898-903. 2005
    ..Thus, Dicer is required for the formation of normal mouse limbs. Strikingly, however, we did not detect defects in basic patterning or in tissue-specific differentiation of Dicer-deficient limb buds...
  79. ncbi Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes
    T Tsuchida
    Howard Hughes Medical Institute Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032
    Cell 79:957-70. 1994
    ....
  80. pmc Evolution acts on enhancer organization to fine-tune gradient threshold readouts
    Justin Crocker
    Department of Biological Sciences, Dartmouth College, Hanover, NH, USA
    PLoS Biol 6:e263. 2008
    ..Our study demonstrates that equivalence classes of CRMs are powerful tools for detecting lineage-specific adaptations by gene regulatory sequences...
  81. pmc In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea
    Ryan S King
    Howard Hughes Medical Institute, Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, 601 South Goodwin Avenue, Urbana, IL 61801, USA
    BMC Dev Biol 13:8. 2013
    ..Whole-mount in situ hybridization (WISH) and whole-mount fluorescent in situ hybridization (FISH) are critical methods for determining gene ..
  82. ncbi Dynamic changes in the response of cells to positive hedgehog signaling during mouse limb patterning
    Sohyun Ahn
    Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA
    Cell 118:505-16. 2004
    ..Finally, by fate mapping Shh-responding cells in Gli2 and Gli3 mutant limbs, we demonstrate that a specific level of positive Hh signaling is not required to specify digit identities...
  83. pmc The T-box transcription factor Tbx18 maintains the separation of anterior and posterior somite compartments
    Markus Bussen
    Institut fur Molekularbiologie, Medizinische Hochschule Hannover, 30625 Hannover, Germany
    Genes Dev 18:1209-21. 2004
    ..In summary, Tbx18 appears to act downstream of Mesp2 and Delta/Notch signaling to maintain the separation of anterior and posterior somite compartments...
  84. ncbi Characterization of progenitor domains in the developing mouse thalamus
    Tou Yia Vue
    Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Comp Neurol 505:73-91. 2007
    ..These results establish the molecular heterogeneity within the progenitor cells of the thalamus, and suggest that such heterogeneity contributes to the specification of thalamic nuclei...
  85. ncbi Origins of bilateral symmetry: Hox and dpp expression in a sea anemone
    John R Finnerty
    Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Science 304:1335-7. 2004
    ..These data suggest that bilateral symmetry arose before the evolutionary split of Cnidaria and Bilateria...
  86. ncbi Wnt11 and Ret/Gdnf pathways cooperate in regulating ureteric branching during metanephric kidney development
    Arindam Majumdar
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Development 130:3175-85. 2003
    ....
  87. pmc Prognostic significance of p16INK4A and human papillomavirus in patients with oropharyngeal cancer treated on TROG 02.02 phase III trial
    Danny Rischin
    Department of Medical Oncology, Peter MacCallum Cancer Centre, A Beckett St, Locked Bag No 1, Melbourne 8006, Australia
    J Clin Oncol 28:4142-8. 2010
    ..To determine the prognostic importance of p16 and human papillomavirus (HPV) in patients with oropharyngeal cancer treated on a phase III concurrent chemoradiotherapy trial...
  88. pmc Ptf1a triggers GABAergic neuronal cell fates in the retina
    Jean Philippe Dullin
    UMR CNRS 8080, Universite Paris Sud, Bat, 445, 91405 Orsay, France
    BMC Dev Biol 7:110. 2007
    ..In the spinal cord and cerebellum, the transcription factor Ptf1a is essential for GABAergic neuron production. In the mouse retina, Ptf1a has been shown to be involved in horizontal and most amacrine neurons differentiation...
  89. ncbi Identification of a Pax6-dependent epidermal growth factor family signaling source at the lateral edge of the embryonic cerebral cortex
    Stavroula Assimacopoulos
    Department of Neurobiology, Pharmacology, and Physiology, The University of Chicago, Chicago, Illinois 60637, USA
    J Neurosci 23:6399-403. 2003
    ..We find that the antihem is lost in mice homozygous for the Small eye (Pax6) mutation and suggest the loss of EGF signaling at least partially explains defects in cortical patterning and cell migration in Small eye mice...
  90. ncbi Immunoglobulin G expression in carcinomas and cancer cell lines
    Zhengshan Chen
    Department of Pathology, School of Basic Medical Sciences, Peking University Health Science Center, 38 Xueyuan Rd, Haidian District, Beijing 100083, China
    FASEB J 21:2931-8. 2007
    ..In surgically resected carcinoma tissues, we detected mRNA of the IGHG1 constant region by in situ hybridization, and by laser microdissection-assisted nested RT-PCR...
  91. ncbi Thalamic development induced by Shh in the chick embryo
    Claudia Vieira
    Neuroscience Institute, Miguel Hernandez University N 332, Km 87, E 03550 Alicante, Spain
    Dev Biol 284:351-63. 2005
    ..Overall, these results indicate that Shh expressed in the ZLI plays an important role in diencephalic growth and in the development of the thalamus...
  92. ncbi Pregnancy and interferon tau regulate RSAD2 and IFIH1 expression in the ovine uterus
    Gwonhwa Song
    Center for Animal Biotechnology and Genomics, Texas A and M University, 442 Kleberg Center, 2471 TAMU, College Station, Texas 77843 2471, USA
    Reproduction 133:285-95. 2007
    ....
  93. ncbi Intestinal growth and differentiation in zebrafish
    Kenneth N Wallace
    Department of Medicine, University of Pennsylvania School of Medicine, Rm 1212, BRB 2 3, 421 Curie Blvd, Philadelphia, PA 19104, USA
    Mech Dev 122:157-73. 2005
    ..Analysis of zebrafish mutants with altered epithelial survival reveals that seemingly related single gene defects have different effects on epithelial differentiation and smooth muscle and enteric nervous system development...
  94. ncbi Tbx1 has a dual role in the morphogenesis of the cardiac outflow tract
    Huansheng Xu
    Program in Cardiovascular Sciences, Baylor College of Medicine, Houston, TX 77030, USA
    Development 131:3217-27. 2004
    ..Furthermore, our data link, for the first time, the function of the secondary heart field to congenital heart disease...
  95. pmc Requirement for hippocampal CA3 NMDA receptors in associative memory recall
    Kazu Nakazawa
    Picower Center for Learning and Memory, RIKEN MIT Neuroscience Research Center, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 297:211-8. 2002
    ..These results provide direct evidence for CA3 NMDA receptor involvement in associative memory recall...
  96. doi High-risk human papillomavirus E6/E7 mRNA detection by a novel in situ hybridization assay strongly correlates with p16 expression and patient outcomes in oropharyngeal squamous cell carcinoma
    Odey C Ukpo
    Department of Pathology and Immunology, Washington University, 660 S Euclid Ave, St Louis, MO 63110, USA
    Am J Surg Pathol 35:1343-50. 2011
    ..causative in oropharyngeal squamous cell carcinomas (OSCCs), being detected in 50% to 80% of tumors by DNA in situ hybridization (ISH) and/or polymerase chain reaction. However, these tests do not assess viral transcription...
  97. pmc Long-range neural and gap junction protein-mediated cues control polarity during planarian regeneration
    Néstor J Oviedo
    Center for Regenerative and Developmental Biology and Department of Biology, Tufts University Suite 4600, 200 Boston Avenue, Medford MA 02155, USA
    Dev Biol 339:188-99. 2010
    ..These data reveal powerful novel controls of pattern formation and suggest a constructive model linking nervous inputs and polarity determination in early stages of regeneration...
  98. ncbi dead end, a novel vertebrate germ plasm component, is required for zebrafish primordial germ cell migration and survival
    Gilbert Weidinger
    Germ Cell Development, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37070 Goettingen, Germany
    Curr Biol 13:1429-34. 2003
    ..We have identified dead end orthologs in other vertebrates including Xenopus, mouse, and chick, where they are expressed in germ plasm and germ-line cells, suggesting a role in germ-line development in these organisms as well...
  99. ncbi Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer
    T Bouwmeester
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, 90095 1737, USA
    Nature 382:595-601. 1996
    ..The results suggest a role for a molecule expressed in the anterior endoderm in the induction of head structures in the vertebrate embryo...
  100. ncbi Localization of leptin receptor mRNA and the long form splice variant (Ob-Rb) in mouse hypothalamus and adjacent brain regions by in situ hybridization
    J G Mercer
    Molecular Neuroendocrinology Group, Rowett Research Institute, Aberdeen
    FEBS Lett 387:113-6. 1996
    Expression of the leptin receptor gene has been examined in mouse hypothalamus and other brain regions by in situ hybridization. With a probe recognizing all the known splice variants, receptor mRNA was evident in several brain regions (..
  101. ncbi Ectodermal Wnt function as a neural crest inducer
    MARTIN I GARCIA-CASTRO
    Division of Biology 139 74, California Institute of Technology, Wilson and California, Pasadena, CA 91125, USA
    Science 297:848-51. 2002
    ..Our data suggest that Wnt molecules are necessary and sufficient to induce neural crest cells in avian embryos...

Research Grants116 found, 100 shown here

  1. Transcription factors regulating sensory gene expression and pain pathways
    Eric E Turner; Fiscal Year: 2011
    ..compound knockout embryos to those from Islet1, Islet2 and Brn3a single knockouts using microarrays, in situ hybridization, and transgenic axon tracing. Aim 2...
  2. Optimization of DNA Repair by Abh8
    Lauren Endres; Fiscal Year: 2013
    ..To assess levels of DNA damage, we will use Fluorescence In Situ Hybridization (FISH) staining with H2AX to detect DNA strand breaks and use COMET assays as a quantitative measurement ..
  3. GENETIC ANALYSIS OF POLARITY
    RUTH M STEWARD; Fiscal Year: 2010
    ..b>In situ hybridization shows that the transcript is uniformly distributed in early embryos suggesting that the specificity comes ..
  4. Unbiased genome-wide screen to identify genes regulating mucous cell hyperplasia
    Jeffrey A Whitsett; Fiscal Year: 2010
    ..from mouse models (in vivo) and in REC cells (in vitro), utilizing QRT-PCR, immunohistochemistry, and in situ hybridization. The nature of the genetic and chemical signaling pathways regulating goblet cell activity, will be ..
  5. Ghrelin: Behavioral, Anatomical, and Physiological Components of Obesity
    BRETT JOHN WARREN TEUBNER; Fiscal Year: 2011
    ..be tested using the retrograde, transynaptic tract tracer, pseudorabies virus (PRV) and visualized using in situ hybridization and immunohistochemistry, respectively...
  6. Estrogen Modulation of Auditory Processing
    Raphael Pinaud; Fiscal Year: 2012
    ..In addition, we aim to understand how this hormone engages sensory-regulated, plasticity-associated gene expression, and ultimately modulates higher-order behaviors such as auditory discrimination. ..
  7. Mechanisms of Angiogenesis in Retinopathy of Prematurity
    Mary Elizabeth Hartnett; Fiscal Year: 2009
    ..cleaved caspase-3) of co-labeled cells; laser capture microdissection;real-time-PCR to quantitate and in situ hybridization to detect location of RNA of VEGF isoforms, VEGF receptors 1 and 2, neuropilins;ELISA and Western blot to ..
  8. NEURAL AND ENDOCRINE CONSEQUENCES OF PARITY
    ROBERT STAFFORD BRIDGES; Fiscal Year: 2012
    ..Techniques used include real time RT-PCR, Western blot analysis, in situ hybridization, radioimmunoassay, and behavioral testing...
  9. Mechanisms of Angiogenesis of Retinopathy of Prematurity
    MARY ELIZABETH RUTH HARTNETT; Fiscal Year: 2013
    ..cleaved caspase-3) of co-labeled cells;laser capture microdissection;real-time-PCR to quantitate and in situ hybridization to detect location of mRNA of VEGF isoforms, VEGF receptors 1 and 2, neuropilins;ELISA and Western blot to ..
  10. RADIATION-INDUCED THYROID CANCER
    Heinz Ulrich G Weier; Fiscal Year: 2011
    ..mRNAs) from candidate genes and expressed sequences in the breakpoint regions using multi-target in situ hybridization analyses...
  11. Developing miRNA diagnostic methods and identifying tumor regulatory networks
    Thomas Tuschl; Fiscal Year: 2010
    ..Second, alterations detected in miRNA expression patterns will be followed by miRNA in situ hybridization, for which we recently developed new small RNA fixation techniques and overcame the biggest obstacle ..
  12. Sex-Hormones and the TMPRSS2: ERG Fusion in Prostate Cancer Progression
    Lorelei Mucci; Fiscal Year: 2013
    ..repository of archival tumor tissue, and will characterize the TMPRSS2:ERG tumor status using fluorescent in situ hybridization. We will explore the role of the TMPRSS2:ERG fusion on PCa progression...
  13. Herpesvirus-induced telomerase dysregulation and tumor formation
    Keith William Jarosinski; Fiscal Year: 2011
    ..their ability for integration and induction chromosome aberrations will be analyzed by using fluorescent in situ hybridization using MDV probes and chicken chromosome paints...
  14. Biofilm Infections in Postsurgical, Trauma, and Critically Ill Patients
    Carol L Wells; Fiscal Year: 2013
    ..methods, confocal microscopy and high resolution electron microscopy, cell sorting, fluorescence in situ hybridization, and biochemical analyses...
  15. Mechanisms of Astrocyte Development
    Teri L Belecky Adams; Fiscal Year: 2010
    ..properties of optic nerve astrocytes in vitro and in vivo using immunohistochemistry, Western blots, in situ hybridization, quantitative polymerase chain reaction and image analysis...
  16. MOLECULAR BIOLOGY OF HEMORRHAGIC SHOCK
    Timothy R Billiar; Fiscal Year: 2013
    ..including immunohistochemistry, confocal microscopy, electron microscopy, quantitative morphology, and in situ hybridization. Since each of the projects tests an individual hypothesis that seeks to identify the molecular events in ..
  17. Localization and characterization of the Clostridium difficile biofilm
    Adam Driks; Fiscal Year: 2013
    ..In Aim 2, we will examine possible mechanisms by which C. difficile biofilms may contribute to CDI. ..
  18. MUCOSAL DEFENSE MECHANISMS IN SUBSTANCE ABUSE
    David R Brown; Fiscal Year: 2010
    ..cells, neurons and leukocytes of Peyer's patch and absorptive mucosae by immunocytochemical and in situ hybridization methods...
  19. Interacting Impact of Adrenal and Ovarian Aging on the CNS
    Henryk F Urbanski; Fiscal Year: 2011
    ..Immunohistochemistry, in situ hybridization histochemistry, RIA and biochemical enzymology will be used to corroborate the results...
  20. Immune Regulation of Viral Recrudescence
    Cornelia Bergmann; Fiscal Year: 2012
    ..b>In situ hybridization combined with confocal microscopy in Aim 1 will determine the spatial relationship between chemokines, ..
  21. Signal Transduction Mechanism of Osteoclast Differentiation
    Nandini Ghosh-Choudhury; Fiscal Year: 2013
    ..transcription factors leading to osteoclast differentiation using immunoblotting, immunohistochemistry, in situ hybridization, ChIP analysis and qRTPCR techniques...
  22. Biomarker Reference Laboratory, EDRN
    William E Grizzle; Fiscal Year: 2010
    ..cytomorphometric analysis, immunohistochemistry (bright field fluorescent, confocal microscopy), in situ hybridization, single and multiplex real time quantitative PCR, other PCR techniques, immunomagnetic separations, tissue ..
  23. RRSS #08: Evaluating Prevalence HPV Infection Among Head and Neck Cancer Patients
    Dennis M Deapen; Fiscal Year: 2009
    ..HPV detection rates is attributable to tumor site, HPV detection method (polymerase chain-reaction (PCR), in situ hybridization (ISH), or Southern hybridization), specimen source and collection methods (swabs, brushings, mouthwash, ..
  24. Developmental Programs of Neural Circuits in the Mouse Medial Hypothalamus
    Holly A Ingraham; Fiscal Year: 2013
    ..2, FezF1 and A2bp1, affects VMH development and function. We will use standard immunohistochemistry, in situ hybridization, and microarray profiling analyses to determine how gene expression and cellular patterns change in ..
  25. Metabolic Pathways and Defects in Fructose Metabolism
    DEAN TOLAN; Fiscal Year: 2009
    ..predictions will be done by quantitative reverse-transcriptase polymerase chain reaction (Q-PCR), RNA in situ hybridization (RISH), and identification of metabolic intermediates during the oxidation of radioactive fructose...
  26. Steroid Regulation of Serotonin in Males
    CYNTHIA LOUISE BETHEA; Fiscal Year: 2013
    ..the regulation of serotonin-related genes TPH2, SERT, 5HT1A, MAO-A and MAO-B will be determined with in situ hybridization. Serotonin fiber density will be measured in the locus coeruleus with immunocytochemistry and stereology ..
  27. Developmental Regulation of Brain and Behavior
    JULI S WADE; Fiscal Year: 2011
    ..expression of these genes or the proteins they encode within developing song control nuclei using in situ hybridization and immunonistochemistry...
  28. The role of satellite rasiRNA in the Segregation Distorter system of Drosophila
    AMANDA MARIE LARRACUENTE; Fiscal Year: 2013
    ..localization or expression by looking for a disruption of Rsp rasiRNAs in SD/SD+ testes using Fluorescence In Situ Hybridization (Aim 2)...
  29. Astrocyte Development and Reactive Gliosis
    Teri L Belecky-Adams; Fiscal Year: 2012
    ..properties of optic nerve astrocytes in vitro and in vivo using immunohistochemistry, Western blots, in situ hybridization, quantitative polymerase chain reaction and image analysis...
  30. microRNAs as novel biomarkers for management of breast cancer
    LORENZO SEMPERE; Fiscal Year: 2012
    ..implemented a sensitive fluorescence-based method that will enable us to co-detect miRNA expression by in situ hybridization (ISH) and protein expression by immunohistochemistry (IHC) in the same tissue section for cell type co-..
  31. Maternal Hyperinsulinemia and Fetal Programming
    Mulchand S Patel; Fiscal Year: 2011
    ..For investigation of alterations in the melanocortin system, in situ hybridization and immunohistochemical analyses will be carried out in brain sections...
  32. Cloning &Functional Studies of Deafness Mutations
    Kenneth R Johnson; Fiscal Year: 2013
    ..anomalies, and temporal and spatial distributions of gene transcripts and proteins will be determined by in situ hybridization and immunohistochemistry...
  33. Rapid Analysis of Single T Cell Immunity Signatures in Tuberculosis
    Sanjay Tyagi; Fiscal Year: 2013
    ..Induced gene expression will be detected by mRNA enumeration using single molecule fluorescence in situ hybridization (smFISH)...
  34. BRAINSTEM MATURATION IN THE SUDDEN INFANT DEATH SYNDROME
    Hannah C Kinney; Fiscal Year: 2010
    ..and the synthetic enzyme, tryptophan hydroxylase, using tissue autoradiography, immunocytochemistry, and in situ hybridization in brain tissues from human embryos, fetuses, and infants...
  35. The Role of Syntrophic Bacteria in Methanogenic Metabolism in the Human Gut
    Catherine Lozupone; Fiscal Year: 2013
    ..smithii using Fluorescence In Situ Hybridization (FISH) and 2) metagenomic sequencing of cell populations that were concentrated using flow cytometry...
  36. Development of quantitative multiplex RNA in situ hybridization
    Thomas Tuschl; Fiscal Year: 2013
    ..Instead of analyzing proteins, mRNA transcripts can be detected by in situ hybridization (ISH), which in principle offers more flexibility in (1) probe synthesis (either as RNA transcript or ..
  37. PAX2 gene targets, binding sites and function
    Patrick D Brophy; Fiscal Year: 2010
    ..screen will be assayed for kidney specific expression during development by whole mount and conventional in situ hybridization. Direct binding of Pax2 to genomic loci will be assayed and the ability of Pax2 to transactivate ..
  38. Tb-FISH (Fluorescent in Situ Hybridization) Assay for Direct Detection of M. tube
    JYOTSNA S SHAH; Fiscal Year: 2010
    ..Based on the feasibility study performed in Phase I, we would like get a FDA approval for the TB-FISH assay. Therefore, in Phase II we shall perform all the studies required by FDA for a PMA filing. ..
  39. The role of the nucleoporin Nup98 in gene regulation
    Martin W Hetzer; Fiscal Year: 2013
    ..addition, a potential effect of Nup98 on mRNA processing and export will be determined by RNA fluorescence in situ hybridization and analysis of recruitment of mRNA processing and nuclear export factors...
  40. Role of Gsalpha in Regulating Osteoblast Differentiation
    JOY Y WU; Fiscal Year: 2012
    ..Aim I seeks to determine how Gsa regulates osteoblast differentiation;this will be assessed in vivo using in situ hybridization, and in vitro by culturing bone marrow stromal cells from wild-type and BGsaKO mice under osteogenic ..
  41. Role of Oleylethanolamine in the Regulation of the HPT Axis
    Csaba Fekete; Fiscal Year: 2010
    ..hormone (TRH) mRNA levels in the hypothalamic paraventricular nucleus (PVN) using semiquantitative in situ hybridization. As existing data indicate that the anorexic effects of OEA are mediated through the brainstem, we will ..
  42. Epigenetic regulation in neuropathic pain
    Zhonghui Guan; Fiscal Year: 2013
    ..My department has guaranteed lab space and 80% of my professional time for my research, neither of which is contingent upon my receipt of this career award. ..
  43. BDNF-Estrogen Interactions with Perimenopausal Mood and Cognition
    Elizabeth M Waters; Fiscal Year: 2012
    ..behavioral measures including open field, object placement and object recognition and quantitative in situ hybridization and light and electron microscopic immunocytochemical methods for the localization of BDNF and TrkB will ..
  44. Genetic and Optogenetic Models to Dissect the Role of the Serotonin 1B Receptor
    Katherine M Nautiyal; Fiscal Year: 2013
    ..Receptor binding and in situ hybridization reveals that these tetO1B mice have normal basal 5-HT1B R expression in the brain compared to 129 WT mice...
  45. Expression control in Drosophila splicing assembly factor RNP-4F
    JACK VAUGHN; Fiscal Year: 2010
    ..correlating the abundance peak of at least one isoform with that for rnp-4f long stem-loop mRNA;(b) using in situ hybridization to demonstrate correlation between the identified dADAR isoform and rnp-4f long stem-loop mRNA;(c) using ..
  46. Gene Networks in Neural Crest-derived Innervation of the Lower Urinary Tract
    E MICHELLE MICHELLE SOUTHARD SMITH; Fiscal Year: 2010
    ..Gene expression patterns derived by in situ hybridization will complement the microarray work and identify key trophic factors and guidance molecules during the ..
  47. microRNA and Down Syndrome
    Karl H Obrietan; Fiscal Year: 2010
    ..Importantly, miRNA expression profiling, miRNA RT-PCR, and miRNA in situ hybridization experiments demonstrated that all five Hsa21-derived miRNAs are over-expressed in brain and heart ..
  48. Prostacyclin Synthase and Receptors in Pulmonary Arterial Hypertension
    Mark W Geraci; Fiscal Year: 2011
    ..PPAR? down-regulation in micro-dissected PAH lesions using methylation specific PCR (MSP) and fluorescence in situ hybridization (FISH)...
  49. Histone Deacetylation Regulates Growth Plate Development
    Lei Wei; Fiscal Year: 2013
    ..MAPK regulates expression or activity of capsases 2 and 3;2c) Runx2 expression is dependent on p38 MAPK by in situ hybridization in p38 MAPK defective and constitutively activated MKK6 mouse growth plates...
  50. Novel Treatment Strategies for Targeting Posttraumatic Epilepsy
    Helen M Bramlett; Fiscal Year: 2013
    ..Quantitative immunocytochemical, western blotting and in situ hybridization approaches for regional and cellular protein and mRNA assessment will be performed to determine these ..
  51. Oocyte-derived R-spondin2 as a Follicle Stimulating Hormone
    AARON JW HSUEH; Fiscal Year: 2013
    ..Based on bioinformatic and in situ hybridization analyses, we demonstrated the exclusive expression of R-spondin2 in oocytes of primary and more advanced ..
  52. Mechanisms of FGFR2 Signaling in Craniofacial Development
    Veraragavan P Eswarakumar; Fiscal Year: 2013
    ..Sutures will be examined by histology, in situ hybridization and by microCT. In Aim 1...
  53. miR132 regulation of neuronal plasticity, learning, and memory
    KATELIN L F HANSEN; Fiscal Year: 2013
    ..To this end, Aim 1 will utilize in situ hybridization and immunolabeling techniques to examine basal miR132 expression patterns within the hippocampus, as well ..
  54. A search for genes that regulate allogeneic stem cell competition
    Irving L Weissman; Fiscal Year: 2013
    ..The expression patterns of selected genes will be validated via qRT-PCR and in situ hybridization, and their function evaluated by knockdown and in vivo engraftment studies...
  55. Germ cell specifications and differentiation in planarians.
    Phillip A Newmark; Fiscal Year: 2013
    ..For both aims, the functional genomic tools for studying S. mediterranea (high- throughput in situ hybridization;RNA interference by feeding double-stranded RNA) will be used to validate target genes and determine their ..
  56. Mechanisms by which VZV IE proteins interact with cell antiviral proteins to prev
    MARIA ACENA NAGEL; Fiscal Year: 2013
    ..at autopsy for interactions of PML-NBs with VZV 61p and VZV 63p using techniques in Aim 1 and fluorescent in situ hybridization to detect VZV DNA in ganglionic neurons;and (Aim 3) analyze human ganglion neurons obtained at autopsy ..
  57. Basis for Lymphomagenesis in Akt2 Transgenic Mice
    Joseph R Testa; Fiscal Year: 2013
    ..Fluorescence in situ hybridization, PCR, and magnetic resonance microimaging analyses will be employed to establish when the inv(6) arises ..
  58. The Role of DNMT3B in the DNA Methylation of Cancer Cells
    LUCY ANN GODLEY; Fiscal Year: 2012
    ..on mouse development by: (A) determining the pattern of DNMT3B7 transgene expression within embryos by in situ hybridization;and (B) examining the effects of DNMT3B7 transgene expression on DNA methylation, histone modifications, ..
  59. Mechanisms of Perchlorate-Induced Disruption of Sexual Differentiation
    FRANK ARTHUR VON HIPPEL; Fiscal Year: 2013
    ..the functional roles of NIS and NIS-paralogs in disruption of gonadal development by perchlorate using in situ hybridization to localize mRNA (Aim 2a), loss-of-function experiments to knock down expression of NIS and NIS-paralogs ..
  60. Base J and transcription termination in Leishmania
    PETER JOHN MYLER; Fiscal Year: 2013
    ..will use in vivo and i vitro transcription assays, genome-wide chromatin mapping, as well as fluorescence in situ hybridization and chromosome conformation capture experiments to determine whether J terminates transcription either by ..
  61. Unconventional Synaptic Modulation at the Vertebrate Neuromuscular Junction
    Clark A Lindgren; Fiscal Year: 2010
    ..by measuring levels of COX mRNA using quantitative PCR and determining its location using fluorescence in situ hybridization. These studies, along with an electrophysiological investigation of NMJs at which the PSCs have been ..
  62. Diabetes Erectile dysfunction and apoptosis of cavernous tissues
    Rajvir Dahiya; Fiscal Year: 2010
    ..We will use the following techniques: RT-PCR, real-time RT-PCR, in situ hybridization, immunohistochemistry, electro-stimulation of cavernous nerve for pressure measurements, gene delivery, ..
  63. Advanced Immunocytochemistry, In Situ Hybridization and Live Cell Imaging Course
    TERRI I GRODZICKER; Fiscal Year: 2013
    ..The Cold Spring Harbor Laboratory proposes to continue a course entitled Advanced Immunocytochemistry, In Situ Hybridization and Live Cell Imaging, to be held in the Fall of 2010-2014...
  64. East African Cichlids: A Natural Model for Dental Regeneration
    RYAN FREDRIC BLOOMQUIST; Fiscal Year: 2013
    ..These findings will advance our understanding of dental stem cells so that we may one day design therapies based on endogenous mechanisms of repair. ..
  65. Nutrient Restriction: Placental and Fetal Brain and Renal Outcomes and Mechanism
    Peter W Nathanielsz; Fiscal Year: 2012
    ..cell signaling and gene function, with global (gene arrays, proteomics) and candidate gene/protein (PCR, in situ hybridization, Western blot and immunohistochemistry) and in vitro (matrigel) and 3) in vivo approaches...
  66. The Role of CLOCK in Ethanol-Related Behaviors
    Angela Renee Ozburn; Fiscal Year: 2012
    ..consumption regulates expression levels of CLOCK in reward- and stress-related regions of the brain using in situ hybridization. Second, we will determine how a dominant negative mutation of CLOCK (CLOCK 19 mice) affects measures of ..
  67. Geobiological approaches to understanding pulmonary infections in situ
    Dianne K Newman; Fiscal Year: 2013
    ..Fluorescence in situ hybridization (FISH) using 16S rRNA-directed probes will enable the spatial organization between different bacterial ..
  68. Circadian Clock Function in the Mammalian Retina
    Christophe P Ribelayga; Fiscal Year: 2013
    ..Using an HPLC technique to measure dopamine levels and in situ hybridization and immunocytochemistry to analyze clock component expression, we will determine 1) whether a clock in the ..
  69. The Role of the Nicotinic Cholinergic Pathway in Retinopathy of Prematurity
    John P Cooke; Fiscal Year: 2013
    ..We will study EC from normal retinal vessels using laser capture microdissection and real time RT-PCR, in situ hybridization and immunohistochemistry...
  70. Cellular and molecular mechanisms of aging and regeneration in colonial chordate
    Irving L Weissman; Fiscal Year: 2013
    ..We will identify the specific cells that show the differential expression by in situ hybridization. This comprehensive screen will be followed by functional experiments that evaluate gene function by ..
  71. Mechanisms of Altered Gastrointestinal Dysfunction
    QiQi Zhou; Fiscal Year: 2013
    ..recognition properties of locked nucleic acid (LNA) modified oligonucleotide probes with fluorescence in situ hybridization (FISH) using tyramide signal amplification (TSA) technology...
  72. polr1c and polr1d mutant zebrafish as new models for Treacher Collins syndrome
    KRISTIN EMILY NOACK WATT; Fiscal Year: 2013
    ..We hypothesize Polr1c and Polr1d function dynamically during embryogenesis which we will demonstrate via in situ hybridization for polr1c and polr1d...
  73. Molecular Pathology of HIV/SIV Enteropathy
    Mahesh Mohan; Fiscal Year: 2013
    ..1c.) Determine cellular localization of differentially expressed miRNAs (in situ hybridization/immunofluorescence) in the colon and verify if differences in expression exist between cell types in close ..
  74. THE ORGANIZATION OF ANIMAL CELL NUCLEI
    Joseph G Gall; Fiscal Year: 2013
    ..We will use immunofluorescent staining and fluorescent in situ hybridization to examine details of RNA transcription and processing at the molecular level...
  75. Hybridization Chain Reaction: In Situ Amplification for Biological Imaging
    Niles A Pierce; Fiscal Year: 2013
    ..b>In situ hybridization methods are an essential tool for elucidating developmental and pathological processes, enabling imaging ..
  76. Smad4 promotes intestinal homeostasis through suppression of Wnt signaling
    Tanner J Freeman; Fiscal Year: 2013
    ..Lgr5, Lrig1, Ascl2, Olfm4, DcamKL1, Mshi1) will be determined by qPCR and localized by IF and/or in situ hybridization. We will confirm loss of Smad4 expression by immunoblotting, IF and qPCR...
  77. Roles for Serotonin in Autonomic Innervation of the Lower Urinary Tract
    KAREN ELAINE RITTER; Fiscal Year: 2013
    ..expression during the development and maturation of LUT autonomic innervation will be determined via in situ hybridization and immunohistochemical localization...
  78. Molecular mechanisms in controlling development of touch-sensing neurons
    Wenqin Luo; Fiscal Year: 2013
    ..We will thoroughly characterize the expression of Er81 in RA mechanoreceptors using in situ hybridization and immunostaining at different developmental stages...
  79. Transcriptional control of collective cell migration
    Lionel Christiaen; Fiscal Year: 2013
    ..TVC-specific whole genome microarray analysis followed by systematic expression and functional analyses by in situ hybridization and targeted over-expression, respectively...
  80. Investigating Cell Specific Roles of the Complement Cascade in Glaucoma
    Gareth R Howell; Fiscal Year: 2013
    ..C1qa([unreadable]Gal) reporter as well as C3, C3R and the inhibitor CRRY using immunofluorescence and RNA in situ hybridization. This is the first time complement proteins have been assessed in two contrasting animal models of ..
  81. Molecular Genetics of Innate Immunity in C. elegans
    Dennis H Kim; Fiscal Year: 2013
    ..in host neuronal gene expression that we have defined quantitatively using single-molecule fluorescent in situ hybridization methods. We have three aims...
  82. Epigenetic regulation of decidual inflammation
    Adrian Erlebacher; Fiscal Year: 2013
    ..Aim III develops a novel histone mark immunostaining/DNA fluorescence in situ hybridization (DNA-FISH) protocol for detecting the decidual gene silencing program at the single cell level so that ..
  83. Patterned Gene Expression in Drosophila Development
    Susan E Celniker; Fiscal Year: 2013
    ....
  84. Plasmodium Genus and P. falciparum - P. vivax FISH Assays
    Jyotsna Shah; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Plasmodium Genus Fluorescent In Situ Hybridization (P-Genus FISH) assay targeted to ribosomal RNA (rRNA) is a method that detects all the species of malaria on an air-dried blood smear...
  85. Non-canonical gene regulation through a Drosophila p53 enhancer
    PAULA SCHLOSSINGER KURTZ; Fiscal Year: 2013
    ..Chromosome conformation capture (3C) as well as fluorescence in situ hybridization (FISH) indicated that this p53RE regulates its target genes through physical interactions by chromatin ..
  86. Midface and Upper Airway in Craniosynostosis
    JOAN THERESE RICHTSMEIER; Fiscal Year: 2013
    ..Finally, using RNA-seq, in situ hybridization, immunoblots, and immunohistochemistry on tissues micro-dissected from specific regions and developmental ..
  87. Detection of Chromosomal Translocations by Single Molecule RNA Imaging
    Mona Batish; Fiscal Year: 2013
    ..We will develop a novel method for the detection of these gene fusion products, which uses fluorescence in situ hybridization probes that bind specifically to different regions of fused mRNAs...
  88. Identification of mechanisms underlying reduction of cocaine seeking by exercise
    YVONNE ENIYE OGBONMWAN; Fiscal Year: 2013
    ..In Specific Aim 1, I will use in situ hybridization, RT-PCR, western blot, and behavioral pharmacology to determine whether (1) chronic voluntary exercise ..
  89. Shear stress, endothelial miRNAs, and AV calcification
    Ajit P Yoganathan; Fiscal Year: 2013
    ..in cultured AVECs and freshly isolated, endothelial-enriched RNA from human and porcine AVs using qPCR and in situ hybridization. The role of these miRNAs in shear-sensitive endothelial function, including inflammation, migration, ..
  90. Gene Discovery in Human Anopthalmia/Micropthalmia
    Anne M Slavotinek; Fiscal Year: 2013
    ....
  91. POSTMORTEM NEUROCHEMICAL STUDIES IN SUICIDE
    Victoria Arango; Fiscal Year: 2013
    ..In Specific Aim 1, we will use in situ hybridization and immunoautoradiography to measure the amount of TPH2 mRNA and protein in the DRN...
  92. Prenatal Exposures & Procarcinogenic Mutations
    Manuela Orjuela; Fiscal Year: 2007
    ..range of chromosome aberrations in cord blood in a small subset of the CCCEH population using fluorescence in situ hybridization (FISH) with whole chromosome painting probes for chromosomes 1-6...
  93. Functional dissection of the Tac2-Nk3R pathway to prevent fear consolidation
    Kerry J Ressler; Fiscal Year: 2013
    ..Specifically, using RNA microarray, qPCR and in situ hybridization approaches, we found that the Tac2 gene is dynamically regulated after cued-fear conditioning in the CeA...
  94. The effects of organophosphates on anxiety-related behavior and neurodevelopment
    Holly Richendrfer; Fiscal Year: 2013
    ..The second aim will be performed using confocal microscopy coupled with in situ hybridization to create 3-D images of larval brain regions using a transgenic line of Zebrafish that express GFP in ..
  95. Generating molecular markers that selectively label urothelial sub-populations
    CATHY LEE MENDELSOHN; Fiscal Year: 2013
    ..Once validated candidates are in hand, we will map their distribution in the LUT using in situ hybridization and immunohistochemistry...
  96. CORE GRANT FOR VISION RESEARCH
    Eric A Newman; Fiscal Year: 2013
    ..and sectioning of tissue, staining of sections, immunohistochemistry, in situ cell death detection, in situ hybridization, Q-PCR analysis of RNA, nuclease protection assays, RNA extraction, and protein assays...
  97. ErbB Family in Pituitary Tumors
    Odelia Cooper; Fiscal Year: 2013
    ..to macroadenomas to increasingly invasive tumors using immunohistochemistry techniques, fluorescent in situ hybridization, and assays for serum EGFR and erbB2...
  98. Dysregulation of RGS2 Protein and Airway Hyperresponsiveness in Asthma
    Yaping Tu; Fiscal Year: 2013
    ..Effects of HDM on miR-1271 and RGS2 expression in mouse airways will be examined by in situ hybridization and immunohistochemistry. We will also determine if IL-13 blockade prevents HDM-effects in vivo...
  99. Heterogeneity of Ventral Tegmental Area Neurons and Opioid Reward
    Elyssa Margolis; Fiscal Year: 2013
    ..recorded neurons will be directly identified as dopamine, glutamate, or GABA using immunocytochemistry or in situ hybridization. Since electrical stimulation of the mPFC is rewarding (Corbett et al...
  100. NEUROPEPTIDES AND THEIR PHYSIOLOGICAL CONTROL
    Alan G Watts; Fiscal Year: 2013
    ..Analytical methods include in situ hybridization, electrophysiology, fluorescence immunocytochemistry, confocal and conventional microscopy, qRT-PCR, and ..
  101. Mapping network connectivity within zebrafish segmentation clock and wavefront
    Scott A Holley; Fiscal Year: 2013
    ..derived from the network model will be tested experimentally via compound gene knockdown, qPCR and in situ hybridization. Network-based explanations for several incongruous phenotypes will be attained...