molecular cloning

Summary

Summary: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Top Publications

  1. ncbi Identification of novel genes coding for small expressed RNAs
    M Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
    Science 294:853-8. 2001
  2. ncbi An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
    N C Lau
    Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 294:858-62. 2001
  3. ncbi Identification of tissue-specific microRNAs from mouse
    Mariana Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Curr Biol 12:735-9. 2002
  4. ncbi A new class of membrane-bound chemokine with a CX3C motif
    J F Bazan
    Department of Molecular Biology, DNAX Research Institute, Palo Alto, California 94304, USA
    Nature 385:640-4. 1997
  5. ncbi An extensive class of small RNAs in Caenorhabditis elegans
    R C Lee
    Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA
    Science 294:862-4. 2001
  6. ncbi HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor
    Y Feng
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases NIAID, NIH, Bethesda, MD 20892, USA
    Science 272:872-7. 1996
  7. ncbi Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction
    Y Y Zhu
    CLONTECH Laboratories, Palo Alto, CA, USA
    Biotechniques 30:892-7. 2001
  8. pmc DNA cloning using in vitro site-specific recombination
    J L Hartley
    Life Technologies, Inc, Rockville, Maryland 20850, USA
    Genome Res 10:1788-95. 2000
  9. pmc A gateway cloning vector set for high-throughput functional analysis of genes in planta
    Mark D Curtis
    Institute of Plant Biology and Zurich Basel Plant Science Centre, University of Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Plant Physiol 133:462-9. 2003
  10. ncbi An improved cyan fluorescent protein variant useful for FRET
    Mark A Rizzo
    Nat Biotechnol 22:445-9. 2004

Detail Information

Publications314 found, 100 shown here

  1. ncbi Identification of novel genes coding for small expressed RNAs
    M Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
    Science 294:853-8. 2001
    ..This suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated...
  2. ncbi An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
    N C Lau
    Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 294:858-62. 2001
    ..The abundance of these tiny RNAs, their expression patterns, and their evolutionary conservation imply that, as a class, miRNAs have broad regulatory functions in animals...
  3. ncbi Identification of tissue-specific microRNAs from mouse
    Mariana Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Curr Biol 12:735-9. 2002
    ..Finally, a miRNA was identified that appears to be the fruitfly and mammalian ortholog of C. elegans lin-4 stRNA...
  4. ncbi A new class of membrane-bound chemokine with a CX3C motif
    J F Bazan
    Department of Molecular Biology, DNAX Research Institute, Palo Alto, California 94304, USA
    Nature 385:640-4. 1997
    ....
  5. ncbi An extensive class of small RNAs in Caenorhabditis elegans
    R C Lee
    Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA
    Science 294:862-4. 2001
    ..elegans larval development, and three of them have apparent homologs in mammals and/or insects. Small noncoding RNAs of the miRNA class appear to be numerous and diverse...
  6. ncbi HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor
    Y Feng
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases NIAID, NIH, Bethesda, MD 20892, USA
    Science 272:872-7. 1996
    ..Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line-tropic isolates, in comparison to its activity with macrophagetropic HIV-1 isolates...
  7. ncbi Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction
    Y Y Zhu
    CLONTECH Laboratories, Palo Alto, CA, USA
    Biotechniques 30:892-7. 2001
    ..cDNA libraries generated using this method will be useful for accelerating the collection of mRNA 5' end sequence information, which is currently very limited in GenBank...
  8. pmc DNA cloning using in vitro site-specific recombination
    J L Hartley
    Life Technologies, Inc, Rockville, Maryland 20850, USA
    Genome Res 10:1788-95. 2000
    ..In this paper, we outline the concepts of this approach and provide several examples that highlight some of its potential...
  9. pmc A gateway cloning vector set for high-throughput functional analysis of genes in planta
    Mark D Curtis
    Institute of Plant Biology and Zurich Basel Plant Science Centre, University of Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Plant Physiol 133:462-9. 2003
    ..All vectors were derived from pCambia T-DNA cloning vectors, with the exception of a chemically inducible vector, for Agrobacterium sp.-mediated transformation of a wide range of plant species...
  10. ncbi An improved cyan fluorescent protein variant useful for FRET
    Mark A Rizzo
    Nat Biotechnol 22:445-9. 2004
    ..Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation...
  11. ncbi A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning
    S Miraglia
    AmCell Corp, Sunnyvale, CA 94089, USA
    Blood 90:5013-21. 1997
    ..This antigen is a glycosylated protein with a molecular weight of 120 kD. Here, we report the molecular cloning of a cDNA encoding this antigen and show that it does not share homology with any previously described ..
  12. ncbi Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
    Mamie Z Li
    Howard Hughes Medical Institute, Department of Genetics, Harvard Partners Center for Genetics and Genomics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Methods 4:251-6. 2007
    ..This flexibility allows much greater versatility in the generation of recombinant DNA for the purposes of synthetic biology...
  13. pmc Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
    L M Guzman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4121-30. 1995
    ..We have exploited the tight regulation of the PBAD promoter to study the phenotypes of null mutations of essential genes and explored the use of pBAD vectors as an expression system...
  14. ncbi Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2
    J Gyuris
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114
    Cell 75:791-803. 1993
    ..These experiments identify Cdi1 as a novel type of protein phosphatase that forms complexes with cyclin-dependent kinases...
  15. ncbi Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation
    T K Vu
    Department of Medicine, University of California, San Francisco 94143 0524
    Cell 64:1057-68. 1991
    ..These data reveal a novel signaling mechanism in which thrombin cleaves its receptor's amino-terminal extension to create a new receptor amino terminus that functions as a tethered ligand and activates the receptor...
  16. ncbi Molecular cloning and cellular localization of a BiP homologue in Trypanosoma brucei. Divergent ER retention signals in a lower eukaryote
    J D Bangs
    Department of Microbiology and Immunology, Stanford School of Medicine, CA 94305 5402
    J Cell Sci 105:1101-13. 1993
    ..1989) Biochemistry 28, 6440-6446), indicates that endoplasmic reticulum retrieval signals in trypanosomes may be as divergent and heterogeneous as any seen in the other eukaryotes yet studied...
  17. ncbi Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein
    Ann M Sheehy
    Department of Microbiology, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nature 418:646-50. 2002
    ..Because the Vif:CEM15 regulatory circuit is critical for HIV-1 replication, perturbing the circuit may be a promising target for future HIV/AIDS therapies...
  18. ncbi Many paths to many clones: a comparative look at high-throughput cloning methods
    Gerald Marsischky
    Institute of Proteomics, Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Boston, Massachusetts 02115, USA
    Genome Res 14:2020-8. 2004
    ..Alternative approaches to creating clones involving mixing of cloning methods, including gap-repair cloning, are also explored...
  19. pmc Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers
    George Adrian Calin
    Department of Microbiology and Immunology, Division of Clinical Pharmacology, Biostatistics Section, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 101:2999-3004. 2004
    ..These data provide a catalog of miR genes that may have roles in cancer and argue that the full complement of miRs in a genome may be extensively involved in cancers...
  20. ncbi Advanced genetic strategies for recombinant protein expression in Escherichia coli
    Hans Peter Sørensen
    Laboratory of BioDesign, Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10 C, DK 8000 Aarhus C, Denmark
    J Biotechnol 115:113-28. 2005
    ..The macromolecular background for a variety of obstacles and genetic state-of-the-art solutions are presented...
  21. ncbi EEA1 links PI(3)K function to Rab5 regulation of endosome fusion
    A Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo
    Nature 394:494-8. 1998
    ....
  22. ncbi hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes
    Philippe Lamesch
    Center for Cancer Systems Biology and Department of Cancer Biology, Dana Farber Cancer Institute, and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Genomics 89:307-15. 2007
    ..dfci.harvard.edu). This expansion of the original ORFeome resource greatly increases the potential experimental search space for large-scale proteomics studies, which will lead to the generation of more comprehensive datasets...
  23. pmc Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs
    Xuezhong Cai
    Box 3025, Duke University Medical Center, Durham, NC 27710, USA
    RNA 10:1957-66. 2004
    ..Together, these data show that human pri-miRNAs are not only structurally similar to mRNAs but can, in fact, function both as pri-miRNAs and mRNAs...
  24. pmc Molecular cloning and expression of glycogen synthase kinase-3/factor A
    J R Woodgett
    Ludwig Institute for Cancer Research, London, UK
    EMBO J 9:2431-8. 1990
    ..Partial purification of GSK-3 activity from bovine brain results in the isolation of active alpha and beta proteins. The physiological importance of these two proteins in cellular signal transduction is discussed...
  25. pmc Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases
    J S Robinson
    Division of Biology, California Institute of Technology, Pasadena 91125
    Mol Cell Biol 8:4936-48. 1988
    ..Together, these data indicate that more than 50 gene products are involved, directly or indirectly, in the process of vacuolar protein sorting...
  26. ncbi Molecular cloning of a novel human CC chemokine liver and activation-regulated chemokine (LARC) expressed in liver. Chemotactic activity for lymphocytes and gene localization on chromosome 2
    K Hieshima
    Department of Biochemistry, Kumamoto University Medical School, Honjo, Kumamoto 860, Japan
    J Biol Chem 272:5846-53. 1997
    ..4 nM and 2100 sites/cell. Collectively, LARC is a novel CC chemokine, which may represent a new group of CC chemokines localized on chromosome 2...
  27. pmc Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers
    K Chang
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4255, USA
    Proc Natl Acad Sci U S A 93:136-40. 1996
    ..The 69-kDa precursor is processed to the 40-kDa form. The protein has been named mesothelin because it is made by mesothelial cells. Mesothelin may play a role in cellular adhesion...
  28. ncbi Molecular cloning of a cDNA encoding a soluble protein in the coral exoskeleton
    Isao Fukuda
    Division of Marine Biosciences, Ocean Research Institute, The University of Tokyo, 1 15 1 Minamidai, Nakano, 168 8639, Tokyo, Japan
    Biochem Biophys Res Commun 304:11-7. 2003
    ..Galaxin exhibits a novel amino acid sequence which is characterized by a tandem repeat structure. Galaxin transcripts were detected in the adult coral, but not in planktonic larvae...
  29. ncbi Molecular cloning and characterization of a novel dopamine receptor (D3) as a target for neuroleptics
    P Sokoloff
    Unité de Neurobiologie et Pharmacologie U 109 de l INSERM, Paris, France
    Nature 347:146-51. 1990
    ..It seems to mediate some of the effects of antipsychotic drugs and drugs used against Parkinson's disease, that were previously thought to interact only with D2 receptors...
  30. ncbi Molecular cloning and characterization of rat LC3A and LC3B--two novel markers of autophagosome
    Jiaxue Wu
    State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China
    Biochem Biophys Res Commun 339:437-42. 2006
    ..Present data suggested that LC3A and LC3B could also be used as two novel autophagosomal markers...
  31. ncbi Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene
    A Poltorak
    Howard Hughes Medical Institute and the Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75235 9050, USA
    Science 282:2085-8. 1998
    ..Destructive mutations of Tlr4 predispose to the development of Gram-negative sepsis, leaving most aspects of immune function intact...
  32. pmc One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, MD 20850, USA
    Proc Natl Acad Sci U S A 105:20404-9. 2008
    ..The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments...
  33. ncbi Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor
    R Eckner
    Dana Farber Cancer Institute, Boston, Massachusetts 02115
    Genes Dev 8:869-84. 1994
    ..These results imply that p300 may function as a transcriptional adaptor protein for certain complex transcriptional regulatory elements...
  34. ncbi Staufen, a gene required to localize maternal RNAs in the Drosophila egg
    D St Johnston
    Max Planck Institut für Entwicklungsbiologie Abteilung III Genetik, Tubingen, Federal Republic of Germany
    Cell 66:51-63. 1991
    ..By the time the egg is laid, staufen protein is also concentrated at the anterior pole, in the same region as bicoid RNA...
  35. pmc Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome
    M Messerle
    Max von Pettenkofer Institut für Hygiene und Mikrobiologie, Ludwig Maximilians Universitat Munchen, Feodor Lynen Strasse 25, D 81377 Munich, Germany
    Proc Natl Acad Sci U S A 94:14759-63. 1997
    ..The described approach should be generally applicable to the mutagenesis of genomes of other large DNA viruses...
  36. ncbi The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins
    M Rothe
    Department of Molecular Biology Tularik, Incorporated, South San Francisco, California 94080, USA
    Cell 83:1243-52. 1995
    ..We report here the biochemical purification and subsequent molecular cloning of two novel TNFR2-associated proteins, designated c-IAP1 and c-IAP2, that are closely related mammalian ..
  37. ncbi Molecular cloning of a lipolysis-stimulated remnant receptor expressed in the liver
    F T Yen
    INSERM Unit 391, 35043 Rennes, France
    J Biol Chem 274:13390-8. 1999
    ..We speculate that LSR is a rate-limiting step for the clearance of dietary triglycerides and plays a role in determining their partitioning between the liver and peripheral tissues...
  38. ncbi Molecular cloning and expression of human hepatocyte growth factor
    T Nakamura
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Nature 342:440-3. 1989
    ..The nucleotide sequence of the human HGF cDNA reveals that both alpha- and beta-chains are contained in a single open reading frame coding for a pre-pro precursor protein of 728 amino acids...
  39. ncbi A genetic screen for mutations affecting embryogenesis in zebrafish
    W Driever
    Cardiovascular Research Center, Massachusetts General Hospital, Charlestown 02129, USA
    Development 123:37-46. 1996
    ..This screen demonstrates that mutations affecting a variety of developmental processes can be efficiently recovered from zebrafish...
  40. ncbi Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is up-regulated in renal cells after injury
    T Ichimura
    Renal Unit, Medical Services, Massachusetts General Hospital East, Boston, Massachusetts 02129, USA
    J Biol Chem 273:4135-42. 1998
    ..KIM-1 may play an important role in the restoration of the morphological integrity and function to postischemic kidney...
  41. ncbi TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus
    P Grüter
    Department of Molecular Biology, University of Geneva, Switzerland
    Mol Cell 1:649-59. 1998
    ..Thus, TAP, like its yeast homolog Mex67p, is a bona fide mRNA nuclear export mediator. TAP is the second cellular RNA binding protein shown to be directly involved in the export of its target RNA...
  42. ncbi Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform
    H Liu
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, D C 20007, USA
    J Biol Chem 275:19513-20. 2000
    ..Triton X-100 inhibited SPHK2 and stimulated SPHK1, whereas phosphatidylserine stimulated both type 1 and type 2 SPHK. Thus, SPHK2 is another member of a growing class of sphingolipid kinases that may have novel functions...
  43. ncbi Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes
    Yi Han Lin
    Laboratory of Biosignals and Bioengineering, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Microbiol 47:849-60. 2003
    ..Thus, AHL-acylases have fundamental implications and hold biotechnological promise in quenching quorum sensing...
  44. pmc Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho
    T Matsui
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan
    EMBO J 15:2208-16. 1996
    ..Thus it is likely that Rho-kinase is a putative target serine/threonine kinase for Rho and serves as a mediator of the Rho-dependent signaling pathway...
  45. pmc Molecular cloning of a potential proteinase activated receptor
    S Nystedt
    Division of Molecular Neurobiology, Wallenberg Laboratory, Lund University, Sweden
    Proc Natl Acad Sci U S A 91:9208-12. 1994
    ..Because this, to our knowledge, is the second example, besides the thrombin receptor, of a proteolytically activated seven-transmembrane G-protein-coupled receptor, we have provisionally named it proteinase activated receptor 2...
  46. pmc A gain-of-function mutation in an Arabidopsis Toll Interleukin1 receptor-nucleotide binding site-leucine-rich repeat type R gene triggers defense responses and results in enhanced disease resistance
    Yumiko Shirano
    Boyce Thompson Institute for Plant Research, Ithaca, New York 14853, USA
    Plant Cell 14:3149-62. 2002
    ....
  47. ncbi Molecular cloning of human cDNA for cathepsin K: novel cysteine proteinase predominantly expressed in bone
    T Inaoka
    International Research Laboratories, Ciba Geigy Japan Ltd, Takarazuka
    Biochem Biophys Res Commun 206:89-96. 1995
    ..These results suggest that cathepsin K is closely involved in human osteoclastic bone resorption...
  48. ncbi Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina
    Gui Min Zhang
    College of Life Science, Hubei University, Wuhan 430062, People s Republic of China
    Appl Microbiol Biotechnol 74:339-46. 2007
    ..It is interesting to note that Cu2+ ion, which strongly inhibits most other xylanases studied, reduces enzyme activity by only 40%. Furthermore, enzyme activity is unaffected by EDTA even at a concentration of 5 mM...
  49. ncbi A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma
    P van der Bruggen
    Ludwig Institute for Cancer Research, Brussels, Belgium
    Science 254:1643-7. 1991
    ..Thus, precisely targeted immunotherapy directed against antigen MZ2-E could be provided to individuals identified by HLA typing and analysis of the RNA of a small tumor sample...
  50. ncbi The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases
    J W Harper
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030
    Cell 75:805-16. 1993
    ..Cotransfection experiments indicate that CIP1 and SV40 T antigen function in a mutually antagonistic manner to control cell cycle progression...
  51. ncbi A component of calcium-activated potassium channels encoded by the Drosophila slo locus
    N S Atkinson
    Laboratory of Genetics, University of Wisconsin, Madison 53706
    Science 253:551-5. 1991
    ..Thus, these results indicate that slo encodes a structural component of Ca(2+)-activated K+ channels...
  52. ncbi Molecular cloning and functional analysis of the mouse homologue of the KILLER/DR5 tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptor
    G S Wu
    Howard Hughes Medical Institute, Department of Medicine, and Cancer Center, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    Cancer Res 59:2770-5. 1999
    ..This is the first report describing a mouse TRAIL receptor gene and also demonstrating that the p53-dependent regulation of KILLER/DR5-mediated apoptosis is conserved between human and mouse...
  53. ncbi A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function
    H Horiuchi
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1149-59. 1997
    ..This demonstrates that a soluble exchange factor coupled to a Rab effector translocates from cytosol to the membrane, where the complex stabilizes the GTPase in the active state...
  54. pmc The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as a key determinant for proanthocyanidin accumulation in developing seed
    N Nesi
    Laboratoire de Biologie des Semences, Unite Mixte de Recherche, Institut National de la Recherche Agronomique, Centre de Versailles, Route de Saint Cyr, 78026 Versailles Cedex, France
    Plant Cell 13:2099-114. 2001
    ..Here the molecular cloning of the TT2 gene was achieved by T-DNA tagging...
  55. pmc USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, Institute of Plant Biology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, Denmark
    Nucleic Acids Res 35:e55. 2007
    ..We expect this method to replace overlapping PCR and the use of type IIS restriction enzymes in many of their applications...
  56. pmc The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase
    T Ishizaki
    Department of Pharmacology, Kyoto University Faculty of Medicine, Japan
    EMBO J 15:1885-93. 1996
    ..When co-expressed with RhoA in COS cells, p160 was co-precipitated with the expressed Rho and its kinase activity was activated, indicating that p160 can associate physically and functionally with Rho both in vitro and in vivo...
  57. ncbi The Rho GTPase and a putative RhoGEF mediate a signaling pathway for the cell shape changes in Drosophila gastrulation
    K Barrett
    Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown 02129, USA
    Cell 91:905-15. 1997
    ..Together, these results establish a Rho-mediated signaling pathway that is essential for the major morphogenetic events in Drosophila gastrulation...
  58. ncbi Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis
    C Heilmann
    Universitat Tubingen, Germany
    Mol Microbiol 20:1083-91. 1996
    ..Inactivation of icaA, icaB, or icaC in pCN27 led to the complete loss of the intercellular adhesion phenotype in S. carnosus, suggesting that all three genes are involved in intercellular adhesion, PIA expression, and translocation...
  59. ncbi Mitochondrial DNA mutations, oxidative stress, and apoptosis in mammalian aging
    G C Kujoth
    Departments of Genetics and Medical Genetics, University of Wisconsin, Madison, WI 53706, USA
    Science 309:481-4. 2005
    ..The levels of apoptotic markers were also found to increase during aging in normal mice. Thus, accumulation of mtDNA mutations that promote apoptosis may be a central mechanism driving mammalian aging...
  60. pmc Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins
    Simon Conn
    Department of Medical Biotechnology, Flinders University, Adelaide, Australia
    J Exp Bot 59:3621-34. 2008
    ..Further investigation of these candidate ligandins may identify a mechanism for manipulating anthocyanin accumulation in planta and in vitro suspension cells...
  61. pmc Molecular cloning and functional characterization of human vesicular glutamate transporter 3
    Shigeo Takamori
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    EMBO Rep 3:798-803. 2002
    ....
  62. pmc Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products
    M N Seaman
    Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla 92093 0668, USA
    J Cell Biol 137:79-92. 1997
    ....
  63. ncbi Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
    Yuen Ho
    MDS Proteomics, 251 Attwell Drive, Toronto, Canada M9W 7H4, and Staermosegaardsvej 6, DK 5230 Odense M, Denmark
    Nature 415:180-3. 2002
    ..Given the high degree of connectivity observed in this study, even partial HMS-PCI coverage of complex proteomes, including that of humans, should allow comprehensive identification of cellular networks...
  64. ncbi An aluminum-activated citrate transporter in barley
    Jun Furukawa
    Research Institute for Bioresources, Okayama University, Chuo, Kurashiki, Okayama, 710 0046, Japan
    Plant Cell Physiol 48:1081-91. 2007
    ..Taken together, our results demonstrate that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barley...
  65. ncbi Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase
    A P Spicer
    Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 272:8957-61. 1997
    ..These results suggest that there are three putative mammalian hyaluronan synthases encoded by three separate but related genes which comprise a mammalian hyaluronan synthase (HAS) gene family...
  66. ncbi TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells
    B R Wong
    The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 272:25190-4. 1997
    ..These results suggest a role for this TNF-related ligand in the regulation of the T cell-dependent immune response...
  67. ncbi A novel multigene family may encode odorant receptors: a molecular basis for odor recognition
    L Buck
    Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Cell 65:175-87. 1991
    ..The members of this novel gene family are likely to encode a diverse family of odorant receptors...
  68. pmc Genes encoding candidate pheromone receptors in a moth (Heliothis virescens)
    J Krieger
    Institute of Physiology 230, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany
    Proc Natl Acad Sci U S A 101:11845-50. 2004
    ..These findings suggest that receptors like Heliothis receptor 14-16 (HR14-HR16) may render antennal cells responsive to pheromones...
  69. ncbi Molecular cloning and localization of a PMCA P-type calcium ATPase from the coral Stylophora pistillata
    Didier Zoccola
    Centre Scientifique de Monaco, Avenue Saint Martin, MC 98000, Monaco
    Biochim Biophys Acta 1663:117-26. 2004
    ..Moreover, this high level of gene conservation further validates the use of cnidarian model systems for studying processes shared by Eumetazoans...
  70. pmc Molecular cloning, sequence, and expression of a human GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase cDNA that can form the H blood group antigen
    R D Larsen
    Howard Hughes Medical Institute, Ann Arbor, MI
    Proc Natl Acad Sci U S A 87:6674-8. 1990
    ..We describe here the molecular cloning, sequence, and expression of a human cDNA corresponding to these human genomic sequences...
  71. pmc Cytosolic triacylglycerol biosynthetic pathway in oilseeds. Molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase
    Saikat Saha
    Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
    Plant Physiol 141:1533-43. 2006
    ..These data suggest that the cytosol is one of the sites for TAG biosynthesis in oilseeds. The identified pathway may present opportunities of bioengineering oil-yielding plants for increased oil production...
  72. ncbi Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 "two-component" osmosensor
    F Posas
    Dana Farber Cancer Institute and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 86:865-75. 1996
    ..We propose that the multistep phosphorelay mechanism is a universal signal transduction apparatus utilized both in prokaryotes and eukaryotes...
  73. ncbi Molecular cloning, characterization, and differential expression of a lanosterol synthase gene from Ganoderma lucidum
    Chang Hua Shang
    College of Life Sciences, Nanjing Agricultural University, Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, Jiangsu, People s Republic of China
    Biosci Biotechnol Biochem 74:974-8. 2010
    ..lucidum during development. Up-regulation of expression of the Gl-LS gene by methyl jasmonate (MeJA) in the mycelia was also demonstrated by real-time RT-PCR. Up-regulation of the Gl-LS promoter activity by MeJA was also investigated...
  74. ncbi Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
    B Karsten Tischer
    Cornell University, Ithaca, NY, USA
    Biotechniques 40:191-7. 2006
    ....
  75. ncbi The late elongated hypocotyl mutation of Arabidopsis disrupts circadian rhythms and the photoperiodic control of flowering
    R Schaffer
    John Innes Centre, Norwich, United Kingdom
    Cell 93:1219-29. 1998
    ..Thus, constant expression of LHY disrupts several distinct circadian rhythms in Arabidopsis, and LHY may be closely associated with the central oscillator of the circadian clock...
  76. ncbi A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA
    E C Lee
    Mouse Cancer Genetics Program, National Cancer Institute Frederick, Frederick, Maryland 21702, USA
    Genomics 73:56-65. 2001
    ....
  77. pmc Emergence of resistant human immunodeficiency virus type 1 in patients receiving fusion inhibitor (T-20) monotherapy
    Xiping Wei
    Howard Hughes Medical Institute, Department of Medicine, University of Alabama at Birmingham, 35294, USA
    Antimicrob Agents Chemother 46:1896-905. 2002
    ..These findings provide the first evidence for the rapid emergence of clinical resistance to a novel class of HIV-1 entry inhibitors and may be relevant to future treatment strategies involving these agents...
  78. ncbi A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis
    T Yokomizo
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Japan
    Nature 387:620-4. 1997
    ..Our findings, together with previous reports, show that LTB4 is a unique lipid mediator that interacts with both cell-surface and nuclear receptors...
  79. ncbi Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides
    A Knappik
    MorphoSys AG, Lena Christ Str 48, Martinsried Munich, 82152, Germany
    J Mol Biol 296:57-86. 2000
    ..The small number of 49 master genes will allow future improvements to be incorporated quickly, and the separation of the frameworks may help in analyzing why nature has evolved these distinct subfamilies of antibody germline genes...
  80. ncbi Molecular cloning and characterization of the intermediate-conductance Ca(2+)-activated K(+) channel in vascular smooth muscle: relationship between K(Ca) channel diversity and smooth muscle cell function
    C B Neylon
    Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA
    Circ Res 85:e33-43. 1999
    ..The differential expression of these K(Ca) channels in functionally distinct smooth muscle cell types suggests that K(Ca) channels play a role in defining the physiological properties of vascular smooth muscle...
  81. ncbi Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs
    Y Okazaki
    1 Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute 1 7 22 Suehiro cho, Tsurumi ku, Yokohama, Kanagawa, 230 0045, Japan
    Nature 420:563-73. 2002
    ..The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics...
  82. ncbi Functional conservation of an insect odorant receptor gene across 250 million years of evolution
    Walton D Jones
    Laboratory of Neurogenetics and Behavior, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Curr Biol 15:R119-21. 2005
    ..The medical and economic impact of these animals can be ascribed in part to the sensitivity and selectivity of their olfactory systems, essential for location of their preferred plant and animal hosts...
  83. ncbi Interaction of tyrosine-based sorting signals with clathrin-associated proteins
    H Ohno
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 269:1872-5. 1995
    ..The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery...
  84. ncbi A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro
    T Hirano
    Department of Pharmacology, University of California, San Francisco 94143 0450
    Cell 79:449-58. 1994
    ..These motifs are highly conserved in a protein family, members of which have been identified recently in both prokaryotes and eukaryotes...
  85. ncbi Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome
    A W Tam
    Molecular Virology Department, Genelabs Incorporated, Redwood City, California 94063
    Virology 185:120-31. 1991
    ..These findings on the genetic organization and expression strategy of HEV suggest that it is the prototype human pathogen for a new class of RNA virus or perhaps a separate genus within the Caliciviridae family...
  86. ncbi Inhibition of death receptor signals by cellular FLIP
    M Irmler
    Institute of Biochemistry, Lausanne Branch, University of Lausanne, Switzerland
    Nature 388:190-5. 1997
    ..High levels of FLIP(L) protein are also detectable in melanoma cell lines and malignant melanoma tumours. Thus FLIP may be implicated in tissue homeostasis as an important regulator of apoptosis...
  87. pmc Molecular cloning of cDNA encoding an unrecognized component of amyloid in Alzheimer disease
    K Ueda
    Department of Neurosciences, School of Medicine, University of California, San Diego, La Jolla 92093 0624
    Proc Natl Acad Sci U S A 90:11282-6. 1993
    ..The availability of the cDNA encoding full-length NACP should help to elucidate the mechanisms of amyloidosis in AD...
  88. ncbi A large-conductance mechanosensitive channel in E. coli encoded by mscL alone
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin Madison 53706
    Nature 368:265-8. 1994
    ..The mscL nucleotide sequence predicts a unique protein of only 136 amino acids, with a highly hydrophobic core and very different from porins or other known proteins...
  89. pmc Identification and characterization of a family of mammalian methyl-CpG binding proteins
    B Hendrich
    Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
    Mol Cell Biol 18:6538-47. 1998
    ..The data demonstrate that MBD2 and MBD4 bind specifically to methyl-CpG in vitro and in vivo and are therefore likely to be mediators of the biological consequences of the methylation signal...
  90. ncbi Molecular cloning of ICAM-3, a third ligand for LFA-1, constitutively expressed on resting leukocytes
    J Fawcett
    ICRF Laboratories, University of Oxford, UK
    Nature 360:481-4. 1992
    ..ICAM-3 is closely related to ICAM-1, consists of five immunoglobulin domains, and binds LFA-1 through its two N-terminal domains...
  91. ncbi Genomic deletions created upon LINE-1 retrotransposition
    Nicolas Gilbert
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Cell 110:315-25. 2002
    ..Thus, our data demonstrate multiple pathways for L1 integration in cultured cells, and show that L1 is not simply an insertional mutagen, but that its retrotransposition can result in significant deletions of genomic sequence...
  92. ncbi The Avr1b locus of Phytophthora sojae encodes an elicitor and a regulator required for avirulence on soybean plants carrying resistance gene Rps1b
    Weixing Shan
    Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA
    Mol Plant Microbe Interact 17:394-403. 2004
    ..Avr1b-2 was genetically mapped to the same BAC contig as Avr1b-1, using a cross between P7064 (race 7) and P6497. The Avr1k gene, required for avirulence on soybean cultivars containing Rps1k, was mapped to the same interval as Avr1b-1...
  93. ncbi Functional interaction of beta-catenin with the transcription factor LEF-1
    J Behrens
    Max Delbruck Centre for Molecular Medicine, Berlin, Germany
    Nature 382:638-42. 1996
    ..Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus...
  94. ncbi A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase
    Xian Jun Song
    National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, The Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
    Nat Genet 39:623-30. 2007
    ..The functional characterization of GW2 provides insight into the mechanism of seed development and is a potential tool for improving grain yield in crops...
  95. ncbi Molecular cloning of matrin 3. A 125-kilodalton protein of the nuclear matrix contains an extensive acidic domain
    P Belgrader
    Department of Biological Sciences, State University of New York, Buffalo 14260
    J Biol Chem 266:9893-9. 1991
    ..The corresponding human deduced partial amino acid sequence is 96% identical to the rat sequence, indicating that matrin 3 is a highly conserved protein...
  96. ncbi The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys
    Matthew Stremlau
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 427:848-53. 2004
    ..Our studies identify TRIM5alpha as a species-specific mediator of innate cellular resistance to HIV-1 and reveal host cell components that modulate the uncoating of a retroviral capsid...
  97. pmc Purification, molecular cloning, and expression of the mammalian sigma1-binding site
    M Hanner
    Instit für Biochemische Pharmakologie, Universitat Innsbruck, Austria
    Proc Natl Acad Sci U S A 93:8072-7. 1996
    ..Northern blots showed high densities of the sigma1-binding site mRNA in sterol-producing tissues. This is also in agreement with the known ability of sigma1-binding sites to interact with steroids, such as progesterone...
  98. ncbi Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily
    C L Mendelsohn
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032
    Cell 56:855-65. 1989
    ....
  99. ncbi Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system
    A Krebber
    Biochemisches Institut der Universitat Zurich, Switzerland
    J Immunol Methods 201:35-55. 1997
    ..To maximize soluble expression of functional scFvs subsequent to cloning, a compatible vector series to simplify modification, detection, multimerization and rapid purification of recombinant antibody fragments was constructed...
  100. ncbi A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells
    R A Black
    Immunex Corporation, Seattle, Washington 98101, USA
    Nature 385:729-33. 1997
    ..Our results should facilitate the development of therapeutically useful inhibitors of TNF-alpha release, and they indicate that an important function of adamalysins may be to shed cell-surface proteins...
  101. pmc Bassoon, a novel zinc-finger CAG/glutamine-repeat protein selectively localized at the active zone of presynaptic nerve terminals
    S tom Dieck
    Leibniz Institute for Neurobiology, D 39118 Magdeburg, Germany
    J Cell Biol 142:499-509. 1998
    ..These data indicate that Bassoon is a strong candidate to be involved in cytomatrix organization at the site of neurotransmitter release...

Research Grants63

  1. The function and regulation of IL-33 in the airway epithelium in asthma
    ERIN D GORDON; Fiscal Year: 2013
    ..They will be ideal in advising me on cell biology, primary airway epithelial cell transfection, molecular cloning, biostatistics, and advanced human genetics...
  2. NEUROVIROLOGY-MOLECULAR BIOLOGY
    Donald Gilden; Fiscal Year: 2013
    ..acid hybridization, molecular genetics, production and characterization of cDNA libraries, DNA sequencing, molecular cloning, virology, production of monoclonal and polyclonal antibodies, cell sorting, quantization of humoral and ..
  3. T Cell-Mediated Mechanisms of Autoimmunity in Murine and Human Biliary Atresia
    CARA LYNN MACK; Fiscal Year: 2011
    ..Specific Aim II is to identify the bile duct epithelial peptide that is the target of T cell activation by molecular cloning from a bile duct epithelial cDNA expression library...
  4. Voltage-Gating in Bacterial Ion Channels
    Ana M Correa; Fiscal Year: 2010
    ..With the advent of high resolution electrical recording techniques combined with the molecular cloning and engineering of ion channel proteins, it has been possible to identify parts of VGC that serve as voltage-..
  5. Norovirus P Particle, A Multifunctional Platform For Vaccine Development
    Ming Tan; Fiscal Year: 2012
    ..Antigens can be inserted onto one to three surface loops of the P domain by molecular cloning. The resulting recombinant protein will spontaneously form chimeric P particle that contain at least 24 ..
  6. Dual Function of VPO1 in Pathogen Recognition and Killing
    Guangjie Cheng; Fiscal Year: 2013
    ..The major methods for addressing the aims include molecular cloning and expressing, protein purification, fluorescence polarization technology and surface plasmon resonance ..
  7. Molecular Genetics of Otolith Formation in the Zebrafish
    Richard Kollmar; Fiscal Year: 2009
    ..The combination of protein purification and sequencing by mass-spectrometry, molecular cloning of cDNAs, in situ hybridization and quantitative RT-PCR, morpholino injections and mutant rescue will lead ..
  8. MOLECULAR CLONING OF EPITHELIAL K CHANNELS
    Henry Sackin; Fiscal Year: 2012
    ..This could ultimately be used for targeted drug design to correct a variety of congenital ion channelopathies affecting the kidney, heart, and pancreas. ..
  9. CELLULAR AND MOLECULAR STUDIES OF RENAL TRANSPORT
    Michael J Caplan; Fiscal Year: 2012
    ..We shall use a wide range of methods including molecular cloning and mutagenesis, functional cDNA expression, generation and use of transgenic mice, immune-cytochemistry, ..
  10. Inpp5f regulates CNS axon regeneration through phosphoinositide metabolism
    Yixiao Zou; Fiscal Year: 2013
    ..The proposed experiments use standard techniques in mouse surgery, immunohistochemistry, biochemistry, molecular cloning, and mouse behavioral assays...
  11. Negative regulation by proteasomal degradation of receptor tyrosine kinase ErbB3
    Ignat Printsev; Fiscal Year: 2013
    ..molecular biology including mammalian tissue culture, immunoprecipitation, western blotting, microscopy, and molecular cloning. The project will be supplemented by regular attendance of scientific presentations on topics in cancer ..
  12. NUCLEAR CONTROL OF RESPIRATORY CHAIN EXPRESSION
    RICHARD SCARPULLA; Fiscal Year: 2012
    ..Our previous studies led to the discovery, purification and molecular cloning of nuclear respiratory factors (NRFs)...
  13. Trafficking and Proteolysis of Notch and Other Gamma-Secretase Substrates
    Mark E Fortini; Fiscal Year: 2012
    ..Further analysis and molecular cloning of several of these new genes is now underway...
  14. MOLECULAR CLONING OF THE FRAGILE X SITE
    Stephen T Warren; Fiscal Year: 2011
    ..Deep fundamental knowledge of a disease is the best course toward eventual therapeutics. ..
  15. Cloning, characterization and in vivo testing of mucosally transmitted SIV
    George M Shaw; Fiscal Year: 2013
    ..This innovation, in turn, makes possible for the first time the molecular cloning and biological analysis of actual transmitted/founder SIV viruses...
  16. KV CHANNEL FUNCTIONING IN MACROMOLECULAR COMPLEXES
    Jeanne M Nerbonne; Fiscal Year: 2010
    ..b>Molecular cloning has provided insights into the basis of neuronal Kv channel diversity with the identification of large ..
  17. AXONAL PATHWAY SELECTION IN THE ZEBRAFISH EMBRYO
    Michael Granato; Fiscal Year: 2013
    ..newly identified genetic players in this process, turn out and rush hour (through cellular experiments and molecular cloning)...
  18. A Combinatorial Approach to Wound Healing for Protein, Gene and Cell Therapeutics
    Andrew Baird; Fiscal Year: 2009
    ..4) identify its receptor by molecular cloning to establish an assay for drug discovery...
  19. Novel SIVsmm strains for analysis of mucosal transmission and vaccine protection
    George M Shaw; Fiscal Year: 2013
    ..Collectively, the projects will test this hypothesis: Identification, molecular cloning, and in vivo analysis of naturally-occurring mucosally-transmitted strains of SIVsmm corresponding to ..
  20. DEFINING INJURIOUS & REPARATIVE MACROPHAGES IN KIDNEY INJURY AND REPAIR
    Jeremy S Duffield; Fiscal Year: 2010
    ..acquisition of skills in proteomics, laser capture microdissection, retroviral gene expression/silencing and molecular cloning. There are accomplished colleagues in the Bonventre laboratory, and nearby at Harvard Medical School who ..
  21. Characterization and Use of Fluorescent Endocannabinoid Transporter Substrates
    Michael Kavanaugh; Fiscal Year: 2009
    ..and kinetics of transport, 2) determine its spatiotemporal distribution in brain, and 3) to identify it by molecular cloning. Characterization and identification of the transporter will provide new strategies for developing potential ..
  22. MOLECULAR BASIS OF GLYCOSPHINGOLIPID BINDING SPECIFICITY
    Dinshaw J Patel; Fiscal Year: 2010
    ..The studies will take advantage of our recent successes in the molecular cloning and expression of GLTP and related point mutants...
  23. CHEMOKINES AND IMMUNE DISEASES
    Lili Feng; Fiscal Year: 2000
    ..Recent advances in molecular cloning of cellular chemoattractants called chemokines have opened a new avenue to determine the means by which the ..
  24. MOLECULAR CLONING OF HLA CLASS II TUMOR ANTIGENS
    Dorothee Herlyn; Fiscal Year: 2000
    ..The antigens also may be of diagnostic value. ..
  25. MOLECULAR CLONING OF HLA CLASS II TUMOR ANTIGENS
    Dorothee Herlyn; Fiscal Year: 2003
    ..The antigens also may be of diagnostic value. ..
  26. MOLECULAR CLONING OF A NOVEL BASEMENT MEMBRANE COMPONENT
    Lawrence Chan; Fiscal Year: 1999
    ..In addition, the candidate has 80% projective time devoted to research. Together, this environment provides sufficient support for the candidate to succeed in his research effort. ..
  27. VITAMIN D HORMONE MECHANISM OF ACTION IN CULTURED CELLS
    MARK HAUSSLER; Fiscal Year: 1999
    ..In the last grant period we accomplished the molecular cloning, overexpression and immunocytochemical localization of the human vitamin D receptor (hVDR), and elucidated ..
  28. New York Nutrition Obesity Research Center
    Xavier Pi-Sunyer; Fiscal Year: 2013
    ..The latter derive from the combination of molecular cloning of rodent obesity genes, sequencing of the mouse and human genomes, introduction of transgenic manipulations,..
  29. STRUCTURE/FUNCTION OF MYOSIN IN SKELETAL MUSCLE
    Gordon Lutz; Fiscal Year: 2001
    ..In this proposal we will use a similar approach (including molecular cloning) to identify and determine the expression patter of MLCs in the various fiber types...
  30. ACTININ ASSOCIATED LIM PROTEIN & FSH MUSCULAR DYSTROPHY
    David Bredt; Fiscal Year: 2002
    ..Recent molecular cloning studies have identified actinin-associated LIM protein (ALP), which is a novel component of the muscle ..
  31. Mechanisms of Neurodegeneration
    Roger J Davis; Fiscal Year: 2010
    ..Many of the components of the JNK protein kinase cascade have been identified by molecular cloning and have been characterized in biochemical studies...
  32. MOLECULAR CLONING AND ANALYSIS OF HUMAN GENOMIC REGIONS
    Donald Moir; Fiscal Year: 1990
    ..Identification of the gene for familial polyposis will be based on screening for detectable alterations in the cDNA or the gene in tissues from affected individuals...
  33. EDITING OF APOLIPOPROTEIN-B MRNA
    DONNA DRISCOLL; Fiscal Year: 2004
    ..a protein that functionally complements apobec-1 and obtained peptide sequence information which was used in molecular cloning experiments. The Apobec-1 Complementation Factor (ACF) cDNA encodes a novel 64...
  34. OPHTHALMOPATHY OF GRAVES DISEASE
    Juan Jaume; Fiscal Year: 2001
    ..His preliminary work in the molecular cloning of autoantibodies in Graves' ophthalmopathy has been a first step in a new approach toward understanding the ..
  35. COMPLEMENTARY DNA PROBES TO A STEROID RECEPTOR GENE
    J Pike; Fiscal Year: 1992
    ..Steroid receptors may represent the best characterized subset of this family primarily because the recent molecular cloning of their respective genes has yielded tremendous structural and functional insight...
  36. Sepsis syndrome and adenosine A2A receptor activation
    WILLIAM SCHELD; Fiscal Year: 2007
    ..Adenosine is a pluripotent molecule released in times of stress such as trauma, ischemia, and infection. Molecular cloning, pharmacological and functional analysis has identified four G protein coupled adenosine receptor (AR) ..
  37. PATHOGENESIS OF FELINE LEUKEMIA VIRUS INDUCED AIDS
    Edward Hoover; Fiscal Year: 1993
    ..By restriction site mapping molecular cloning, DNA sequence analysis, and in vitro and in vivo analysis of constructed viral chimeras, we have identified ..
  38. THYROID PEROXIDASE AS AN AUTOANTIGEN
    Basil Rapoport; Fiscal Year: 2005
    ..2. MOLECULAR CLONING OF NEW TPO AUTOANTIBODIES Human monoclonal autoantibodies are key reagents for defining B and T cell ..
  39. MOLECULAR BIOLOGY OF BRAIN PROTEIN PHOSPHORYLATION
    James Sikela; Fiscal Year: 1993
    ..2) A novel molecular cloning strategy, homology probing will be utilized to identify genes encoding previously uncharacterized protein-..
  40. HETEROGENEITY OF SYNAPTIC NMDA RECEPTORS
    Stefano Vicini; Fiscal Year: 2002
    ..Recent developments in molecular cloning of NMDA receptors have revealed a substantial heterogeneity of molecular forms of these receptors...
  41. Molecular Toxicology of Carboxylesterases
    Bingfang Yan; Fiscal Year: 2006
    ..The focus of the current grant period has been on molecular cloning, enzyme kinetics and xenobiotic regulation...
  42. RECOGNITION OF HLA-A2 MOLECULE BY HUMAN CYTOTOXIC T CELL
    Craig Hall; Fiscal Year: 1992
    ..The goals of the proposed project are to determine by molecular cloning the structures of T cell receptors from a series of alloreactive human cytotoxic T cell clones specific for ..
  43. NATURE OF HIV-RELATED GENETIC SEQUENCES IN HUMAN DNA
    ANTHONY FARAS; Fiscal Year: 1991
    Employing sensitive hybridization and molecular cloning techniques that allow identification and isolation of nucleotide sequences exhibiting as little as 60-70% homology to the probes employed, we have identified novel families of ..
  44. Extracellular Modulation of Metabotropic GluRs
    Julie Saugstad; Fiscal Year: 2005
    ..b>Molecular cloning of mGluRs revealed eight distinct receptor subtypes: Group I mGluRs (mGluRs 1 and 5) couple to the Gq ..
  45. CONSTITUTIVELY ACTIVE PTH/PTHRP RECEPTORS IN VIVO
    HARALD JUEPPNER; Fiscal Year: 2003
    DESCRIPTION (Verbatim from the Applicant): After the molecular cloning of the PTH/PTHrP receptor (PTH1R) and its gene, we identified activating mutations in Jansen's metaphyseal chondrodysplasia (JMC), a genetic disorder characterized ..
  46. MOLECULAR CLONING OF THE HUMAN SWEET TASTE RECEPTOR
    MYLES AKABAS; Fiscal Year: 1992
    ..The transduction of sweet taste appears to involve a different mechanism. Purification or molecular cloning of the taste receptors would enhance our understanding of the primary events in taste transduction, however, ..
  47. DNA POLYMERASES IN NORMAL AND CANCER CELLS
    LUCY CHANG; Fiscal Year: 1991
    ..The specific aims are as follow: 1. Molecular cloning of DNA polymerase-beta: cDNA clones of mammalian DNA polymerase-beta will be isolated in order to examine ..
  48. Molecular Cloning of the Wilms tumor Gene from 7p15-21
    Khalid Sossey Alaoui; Fiscal Year: 2004
    ..Understanding the nature of the genetic events which allow these cells to escape their normal growth regulation may also provide an opportunity for therapeutic intervention. ..
  49. MOLECULAR AND EVOLUTIONARY GENETICS OF SEGREGATION
    CHUNG WU; Fiscal Year: 1992
    ..We are now ready to study the molecular basis of SD. Molecular cloning will also lead to a better understanding of the population genetics of this system...
  50. FKBP12 IN TGF-BETA TYPE I RECEPTOR MEDIATED SIGNALING
    Tongwen Wang; Fiscal Year: 1999
    ..b>Molecular cloning and characterization of both the type I and type II receptors of TGF-beta family members revealed that ..
  51. MOLECULAR CLONING OF THE DR7-ASSOCIATED IA GENES
    Robert Karr; Fiscal Year: 1992
    ..The findings of these studies will contribute to the understanding of the diversity of human Ia molecules and the function of immune response genes, which may play important roles in the determination of human health and disease...
  52. XEN-DORPHINS--A NEW OPIOID SYSTEM
    Srinivasa Nagalla; Fiscal Year: 2001
    ..as analgesia and complex behavioral effects like tolerance and dependence has been greatly facilitated by molecular cloning of opioid receptors and ligands...
  53. MOLECULAR CLONING OF BRAIN MRNA IN ETHANOL DEPENDENCE
    STEVEN SIGNS; Fiscal Year: 1991
    ..The molecular cloning techniques of "differential colony hybridization" and "subtracted library hybridization" will be used to ..
  54. TRAFFICKING OF ADH WATER CHANNELS IN TIGHT EPITHELIA
    H Harris; Fiscal Year: 2000
    ..b>Molecular cloning of the ADH elicited water channel called Aquaporin (AQP) 2 has demonstrated that ADH regulates Pf via ..
  55. ZNF217 MEDIATED CELLULAR IMMORTALIZATION IN BREAST CANCE
    Colin Collins; Fiscal Year: 2001
    ..A positional cloning strategy was employed to identify the hypothesized oncogene that culminated in the molecular cloning of ZNF217...
  56. MOLECULAR CLONING OF A T(15;19) IN LUNG CANCER CELL LINE
    Thao Dang; Fiscal Year: 2005
    ..The support given by this K08 award will allow Dr. Dang to build on her existing knowledge and promote her transition to an independent investigator in a highly competitive environment. ..
  57. Role of Kir6.1 subunits of K+ channels in heart
    WILLIAM COETZEE; Fiscal Year: 2004
    ..The molecular cloning of subunits of KATP channel revealed that KATP channels consist of pore-forming Kir6 subunits in association ..
  58. Viral Etiology of Kaposi's sarcoma-like Tumors
    TIMOTHY ROSE; Fiscal Year: 2005
    ..There, Dr. Rose set up molecular cloning and bioinformatics and focused his research on the identification of new growth factors...
  59. REGULATION OF COLONIC H-K-ATPASE IN THE KIDNEY
    Manoocher Soleimani; Fiscal Year: 2001
    ..Insight into regulation of cHKA will significantly enhance our knowledge on a variety of pathologic conditions associated with electrolyte and acid-base abnormalities. ..
  60. Polyamine metabolism in heart and kidney ischemic injury
    Manoocher Soleimani; Fiscal Year: 2005
    ..abstract_text> ..
  61. Apical CI/OH-/HCO3-exchanger in kidney and GI tract
    Manoocher Soleimani; Fiscal Year: 2007
    ..They should further facilitate future investigations into the role of this transporter in pathophysiologic states affecting electrolyte and acid-base homeostasis. ..
  62. CHARACTERIZATION OF NA-DEPENDENT HCO3-TRANSPORTERS
    Manoocher Soleimani; Fiscal Year: 2001
    ..Insight into regulation and gene structure of Na-coupled HCO3 transporters should enhance our knowledge on cell regulation and acid-base homeostasis. ..