genetic complementation test

Summary

Summary: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.

Top Publications

  1. pmc Preservation of duplicate genes by complementary, degenerative mutations
    A Force
    Department of Biology, University of Oregon, Eugene, Oregon 97403, USA
    Genetics 151:1531-45. 1999
  2. ncbi A nonribosomal peptide synthetase gene (mgoA) of Pseudomonas syringae pv. syringae is involved in mangotoxin biosynthesis and is required for full virulence
    Eva Arrebola
    Departamento de Microbiologia, Facultad de Ciencias, Universidad de Malaga, Malaga, Spain
    Mol Plant Microbe Interact 20:500-9. 2007
  3. ncbi Evidence for autolysin-mediated primary attachment of Staphylococcus epidermidis to a polystyrene surface
    C Heilmann
    Institut fur Medizinische Mikrobiologie, Munster, Germany
    Mol Microbiol 24:1013-24. 1997
  4. ncbi COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility
    D X Xie
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Science 280:1091-4. 1998
  5. pmc Development and application of an insertional system for gene delivery and expression in Campylobacter jejuni
    A V Karlyshev
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, Keppel St, London WC1E 7HT, United Kingdom
    Appl Environ Microbiol 71:4004-13. 2005
  6. ncbi Signal transduction mutants of Arabidopsis uncouple nuclear CAB and RBCS gene expression from chloroplast development
    R E Susek
    Plant Biology Laboratory, Salk Institute for Biological Studies, San Diego, California 92186 5800
    Cell 74:787-99. 1993
  7. ncbi Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway
    I Garcia-Higuera
    Department of Pediatric Oncology, Dana Farber Cancer Institute, and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 7:249-62. 2001
  8. pmc Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals
    Kathryn E Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 101:12288-93. 2004
  9. pmc Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammals
    Dorothee Grimm
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 101:3142-7. 2004
  10. ncbi Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions
    Shoko Kojima
    Institute of the Society for Techno Innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki, 305 0854 Japan
    Plant Cell Physiol 43:1096-105. 2002

Detail Information

Publications317 found, 100 shown here

  1. pmc Preservation of duplicate genes by complementary, degenerative mutations
    A Force
    Department of Biology, University of Oregon, Eugene, Oregon 97403, USA
    Genetics 151:1531-45. 1999
    ..Cooke et al. 1997 (p. 362)..
  2. ncbi A nonribosomal peptide synthetase gene (mgoA) of Pseudomonas syringae pv. syringae is involved in mangotoxin biosynthesis and is required for full virulence
    Eva Arrebola
    Departamento de Microbiologia, Facultad de Ciencias, Universidad de Malaga, Malaga, Spain
    Mol Plant Microbe Interact 20:500-9. 2007
    ..syringae pv. syringae. The involvement of an NRPS in the production of an antimetabolite toxin from P. syringae inhibiting ornithine acetyltransferase activity is proposed...
  3. ncbi Evidence for autolysin-mediated primary attachment of Staphylococcus epidermidis to a polystyrene surface
    C Heilmann
    Institut fur Medizinische Mikrobiologie, Munster, Germany
    Mol Microbiol 24:1013-24. 1997
    ..Our findings provide evidence for a new function of an autolysin (AtlE) in mediating the attachment of bacterial cells to a polymer surface, representing the prerequisite for biofilm formation...
  4. ncbi COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility
    D X Xie
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Science 280:1091-4. 1998
    ..It has similarity to the F-box proteins Arabidopsis TIR1, human Skp2, and yeast Grr1, which appear to function by targeting repressor proteins for removal by ubiquitination...
  5. pmc Development and application of an insertional system for gene delivery and expression in Campylobacter jejuni
    A V Karlyshev
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, Keppel St, London WC1E 7HT, United Kingdom
    Appl Environ Microbiol 71:4004-13. 2005
    ..The principles developed in this study could be more widely applied for the manipulation of other bacteria that are refractory to genetic analysis...
  6. ncbi Signal transduction mutants of Arabidopsis uncouple nuclear CAB and RBCS gene expression from chloroplast development
    R E Susek
    Plant Biology Laboratory, Salk Institute for Biological Studies, San Diego, California 92186 5800
    Cell 74:787-99. 1993
    ..Other reports suggest this intracellular cross-talk also involves mitochondrial interactions. The GUN genes thus define steps in one specific branch of a complex interorganellar regulatory network...
  7. ncbi Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway
    I Garcia-Higuera
    Department of Pediatric Oncology, Dana Farber Cancer Institute, and Department of Pediatrics, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 7:249-62. 2001
    ..The FANCD2 protein, therefore, provides the missing link between the FA protein complex and the cellular BRCA1 repair machinery. Disruption of this pathway results in the cellular and clinical phenotype common to all FA subtypes...
  8. pmc Kinetics of regulated protein-protein interactions revealed with firefly luciferase complementation imaging in cells and living animals
    Kathryn E Luker
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 101:12288-93. 2004
    ....
  9. pmc Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammals
    Dorothee Grimm
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 101:3142-7. 2004
    ..The induction of a spirochetal virulence factor preceding the time and host in which it is required demonstrates a developmental sequence for transmission of this arthropod-borne pathogen...
  10. ncbi Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions
    Shoko Kojima
    Institute of the Society for Techno Innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki, 305 0854 Japan
    Plant Cell Physiol 43:1096-105. 2002
    ....
  11. pmc Disruption of OsYSL15 leads to iron inefficiency in rice plants
    Sichul Lee
    Department of Integrative Bioscience and Biotechnology, Pohang University of Science and Technology, Pohang 790 784, Republic of Korea
    Plant Physiol 150:786-800. 2009
    ..Overexpression of OsYSL15 increased the Fe concentration in leaves and seeds from transgenic plants. Altogether, these results demonstrate roles for OsYSL15 in Fe uptake and distribution in rice plants...
  12. pmc A novel gene inactivation system reveals altered periplasmic flagellar orientation in a Borrelia burgdorferi fliL mutant
    M A Motaleb
    Department of Microbiology and Immunology, East Carolina University, Greenville, NC 27834, USA
    J Bacteriol 193:3324-31. 2011
    ..Our results suggest that FliL is likely involved in coordinating or regulating the orientation of periplasmic flagella in B. burgdorferi...
  13. ncbi The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio
    P Haffter
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Genetik, Tubingen, Germany
    Development 123:1-36. 1996
    ..Here we give an overview of the spectrum of mutant phenotypes obtained, and discuss the limits and the potentials of a genetic saturation screen in the zebrafish...
  14. ncbi Identification of a protein required for disulfide bond formation in vivo
    J C Bardwell
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Cell 67:581-9. 1991
    ..The purified DsbA protein is capable of reducing the disulfide bonds of insulin, an activity that it shares with these disulfide oxidoreductases. Our results suggest that disulfide bond formation is facilitated by DsbA in vivo...
  15. pmc The acyl-CoA synthetase encoded by LACS2 is essential for normal cuticle development in Arabidopsis
    Judy Schnurr
    Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164, USA
    Plant Cell 16:629-42. 2004
    ..The LACS2 gene and the corresponding lacs2 mutant will help in future studies of the cutin synthesis pathway and in understanding the consequences of reduced cutin production on many aspects of plant biology...
  16. pmc Contribution of horizontal gene transfer to the evolution of Saccharomyces cerevisiae
    Charles Hall
    Department of Molecular Genetics and Microbiology, Duke University Medical Center DUMC 289 CARL Building, Box 3568, Durham, NC 27710, USA
    Eukaryot Cell 4:1102-15. 2005
    ..Among the eukaryotes, this gene is found in S. cerevisiae and Saccharomyces bayanus and appears to derive from the alpha-proteobacteria...
  17. pmc Borrelia burgdorferi sigma54 is required for mammalian infection and vector transmission but not for tick colonization
    Mark A Fisher
    Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 102:5162-7. 2005
    ..However, sigma54 mutants did not enter the salivary glands during tick feeding, indicating that sigma54-regulated genes were involved in the transmission process...
  18. ncbi Functional discovery via a compendium of expression profiles
    T R Hughes
    Rosetta Inpharmatics, Inc, Kirkland, Washington 98034, USA
    Cell 102:109-26. 2000
    ..We also show that the compendium can be used to characterize pharmacological perturbations by identifying a novel target of the commonly used drug dyclonine...
  19. pmc The beta-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes
    Hemayet Ullah
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3280, USA
    Plant Cell 15:393-409. 2003
    ..These results suggest that although auxin-regulated cell division is not coupled directly by a G protein, the Gbeta-subunit attenuates this auxin pathway upstream of the control of mRNA steady state levels...
  20. ncbi Activation tagging of the floral inducer FT
    I Kardailsky
    Plant Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 286:1962-5. 1999
    ..Instead, it is similar to the sequence of TERMINAL FLOWER 1 (TFL1), an inhibitor of flowering that also shares sequence similarity with membrane-associated mammalian proteins...
  21. ncbi Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na unloading from xylem vessels to xylem parenchyma cells
    - Sunarpi
    Bioscience and Biotechnology Center, Nagoya University, Nagoya 464 8601, Japan
    Plant J 44:928-38. 2005
    ..Thus AtHKT1 reduces the sodium content in xylem vessels and leaves, thereby playing a central role in protecting plant leaves from salinity stress...
  22. pmc Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae
    J P Radicella
    Laboratoire de Radiobiologie du DNA, Commissariat a l Energie Atomique, Departement de Radiobiologie et Radiopathologie, UMR217 Centre National de la Recherche Scientifique, 92265 Fontenay aux Roses, France
    Proc Natl Acad Sci U S A 94:8010-5. 1997
    ....
  23. pmc L,L-diaminopimelate aminotransferase, a trans-kingdom enzyme shared by Chlamydia and plants for synthesis of diaminopimelate/lysine
    Andrea J McCoy
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 4799, USA
    Proc Natl Acad Sci U S A 103:17909-14. 2006
    ....
  24. ncbi The Arabidopsis ABORTED MICROSPORES (AMS) gene encodes a MYC class transcription factor
    Anna Marie Sorensen
    Department of Molecular Plant Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany
    Plant J 33:413-23. 2003
    ..Therefore, AMS plays a crucial role in tapetal cell development and the post-meiotic transcriptional regulation of microspore development within the developing anther...
  25. ncbi Multiple functions of Pmt1p-mediated protein O-mannosylation in the fungal pathogen Candida albicans
    C Timpel
    Institut für Mikrobiologie und Biologisch Medizinisches Forschungszentrum, Heinrich Heine Universitat, D 40225 Dusseldorf, Germany
    J Biol Chem 273:20837-46. 1998
    ..The results indicate that protein O-mannosylation by Pmt proteins occurs in different fungal species, where PMT1 deficiency can lead to defects in multiple cellular functions...
  26. ncbi Split luciferase complementation assay to study protein-protein interactions in Arabidopsis protoplasts
    Yukichi Fujikawa
    Department of Biological Sciences, Louisiana State University, 226 Life Sciences Building, Baton Rouge, LA 70803 1715, USA
    Plant J 52:185-95. 2007
    ..As proof of concept, we detected the protein-protein interactions between the nuclear histones 2A and 2B, as well as between membrane proteins SYP (syntaxin of plant) 51 and SYP61, in Arabidopsis protoplasts...
  27. pmc SleC is essential for germination of Clostridium difficile spores in nutrient-rich medium supplemented with the bile salt taurocholate
    David A Burns
    Centre for Biomolecular Sciences, School of Molecular Medical Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    J Bacteriol 192:657-64. 2010
    ..Furthermore, fewer R20291 spores germinated, indicating that there are differences in both sporulation and germination between these epidemic and nonepidemic C. difficile isolates...
  28. ncbi A chemical complementation approach reveals genes and interactions of flavonoids with other pathways
    Lucille Pourcel
    Center for Applied Plant Sciences and Department of Molecular Genetics, Ohio State University, Columbus, OH 43210, USA
    Plant J 74:383-97. 2013
    ..Taken together, our data suggest that Arabidopsis can sense flavonoids as a signal for multiple fundamental cellular processes...
  29. ncbi Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast
    Tadashi Mutoh
    Graduate Program in Biophysics and Biochemistry, School of Science, University of Tokyo, Hongo, Bunkyo ku, Japan
    Genes Cells 10:1189-202. 2005
    ..Rho1 is likely to be activated by Rgf3 at the division site, and involved in contractile ring formation and/or maintenance and septation...
  30. pmc Manganese efficiency in barley: identification and characterization of the metal ion transporter HvIRT1
    Pai Pedas
    Plant and Soil Science Laboratory, Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 148:455-66. 2008
    ..We conclude that HvIRT1 is an important component controlling Mn(2+) uptake in barley roots and contributes to genotypic differences in Mn(2+) uptake kinetics...
  31. ncbi AgrD-dependent quorum sensing affects biofilm formation, invasion, virulence and global gene expression profiles in Listeria monocytogenes
    Christian U Riedel
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Mol Microbiol 71:1177-89. 2009
    ..The results presented here suggest that peptide sensing plays an important role in the biology of L. monocytogenes, with relevant phenotypes in both the saprophytic and parasitic lifecycles...
  32. pmc Borrelia burgdorferi uniquely regulates its motility genes and has an intricate flagellar hook-basal body structure
    Melanie S Sal
    Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Robert C Byrd Health Sciences Center, Morgantown, WV 26506 9177, USA
    J Bacteriol 190:1912-21. 2008
    ..In addition, we also present evidence that the hook protein in B. burgdorferi forms a high-molecular-weight complex and that formation of this complex occurs in the periplasmic space...
  33. ncbi MoSNF1 regulates sporulation and pathogenicity in the rice blast fungus Magnaporthe oryzae
    Mihwa Yi
    Department of Agricultural Biotechnology, Center for Fungal Genetic Resources and Center for Agricultural Biomaterials, Seoul National University, Seoul 151 921, Republic of Korea
    Fungal Genet Biol 45:1172-81. 2008
    ..Our results suggest that MoSNF1 is a functional homolog of yeast Snf1, but its contribution to sporulation, vegetative growth and pathogenicity is critical in M. oryzae...
  34. pmc The heme biosynthetic pathway of the obligate Wolbachia endosymbiont of Brugia malayi as a potential anti-filarial drug target
    Bo Wu
    Division of Molecular Parasitology, New England Biolabs, Ipswich, Massachusetts, United States of America
    PLoS Negl Trop Dis 3:e475. 2009
    ..Human trials have confirmed this approach, although the length of treatments, high doses required and medical counter-indications for young children and pregnant women warrant the identification of additional anti-Wolbachia drugs...
  35. ncbi Deregulation of a Ca2+/calmodulin-dependent kinase leads to spontaneous nodule development
    Leïla Tirichine
    Nature 441:1153-6. 2006
    ..Our results reveal a key regulatory position of CCaMK upstream of all components required for cell-cycle activation, and a phenotypically divergent series of mutant alleles demonstrates positive and negative regulation of the process...
  36. ncbi Virulence regulator PrfA is essential for biofilm formation in Listeria monocytogenes but not in Listeria innocua
    Qingchun Zhou
    Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Luo Yu Road 152, Wuhan, People s Republic of China
    Curr Microbiol 63:186-92. 2011
    ..Our results suggest that PrfA plays a significant role in biofilm formation in L. monocytogenes but not in L. innocua, thus may reflect differences in the molecular mechanisms of biofilm formation by these two closely related species...
  37. ncbi MbtH-like protein-mediated cross-talk between non-ribosomal peptide antibiotic and siderophore biosynthetic pathways in Streptomyces coelicolor M145
    Sylvie Lautru
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Microbiology 153:1405-12. 2007
    ..Transcriptional analyses of wild-type S. coelicolor and a double cchK/cdaX replacement mutant indicate that CchK and CdaX may not be involved in transcriptional regulation of coelichelin and CDA biosynthetic gene clusters...
  38. ncbi Molecular characterization of PoGT8D and PoGT43B, two secondary wall-associated glycosyltransferases in poplar
    Gong Ke Zhou
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 48:689-99. 2007
    ..Taken together, our results demonstrate that PoGT8D and PoGT43B are Golgi-localized, secondary wall-associated proteins, and PoGT43B is a functional ortholog of IRX9 involved in GX biosynthesis during wood formation...
  39. pmc A dominant function of CCaMK in intracellular accommodation of bacterial and fungal endosymbionts
    Teruyuki Hayashi
    National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305 8602, Japan
    Plant J 63:141-54. 2010
    ..We propose a model of symbiotic signaling in host legume plants, in which CCaMK plays a key role in the coordinated induction of infection thread formation and nodule organogenesis...
  40. ncbi NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis
    D Rentsch
    Institut fur Genbiologische Forschung, Berlin, Germany
    FEBS Lett 370:264-8. 1995
    ..In siliques NTR1-expression is restricted to the embryo, implicating a role in the nourishment of the developing seed...
  41. pmc Regulation of dev, an operon that includes genes essential for Myxococcus xanthus development and CRISPR-associated genes and repeats
    Poorna Viswanathan
    Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
    J Bacteriol 189:3738-50. 2007
    ..xanthus...
  42. pmc Massetolide A biosynthesis in Pseudomonas fluorescens
    I de Bruijn
    Laboratory of Phytopathology, Wageningen University, The Netherlands
    J Bacteriol 190:2777-89. 2008
    ..Massetolide A production is essential in swarming motility of P. fluorescens SS101 and plays an important role in biofilm formation...
  43. pmc An unusual response regulator influences sporulation at early and late stages in Streptomyces coelicolor
    Yuqing Tian
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People s Republic of China
    J Bacteriol 189:2873-85. 2007
    ..Consideration of the functions of whiI-influenced genes provides some insights into the physiology of aerial hyphae. Mutation of seven whiI-influenced genes revealed that three of them play roles in spore maturation...
  44. pmc Loss-of-function and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis
    Tomoko Hirano
    Graduate School of Biostudies, Kyoto University, Kyoto 606 8501, Japan
    Plant Physiol 155:797-807. 2011
    ....
  45. pmc RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle
    Benjamin Albert
    Laboratoire de Biologie Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique, Universite de Toulouse, F 31000 Toulouse, France
    J Cell Biol 192:277-93. 2011
    ..Together our data suggest that localized rRNA production results in spatially constrained rRNA production, which is instrumental for nucleolar assembly...
  46. pmc Requirement of the galU gene for polysaccharide production by and pathogenicity and growth In Planta of Xanthomonas citri subsp. citri
    Yinping Guo
    Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850, USA
    Appl Environ Microbiol 76:2234-42. 2010
    ..1 containing an intact galU gene. These data indicate that the galU gene contributes to X. citri subsp. citri growth in intercellular spaces and is involved in EPS and CPS synthesis and biofilm formation...
  47. ncbi An ancient light-harvesting protein is critical for the regulation of algal photosynthesis
    Graham Peers
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 3102, USA
    Nature 462:518-21. 2009
    ..Thus, these data indicate that plants and algae use different proteins to dissipate harmful excess light energy and protect the photosynthetic apparatus from damage...
  48. ncbi BnaC.Tic40, a plastid inner membrane translocon originating from Brassica oleracea, is essential for tapetal function and microspore development in Brassica napus
    Xiaoling Dun
    National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, Huazhong Agricultural University, Wuhan 430070, China
    Plant J 68:532-45. 2011
    ..These data indicate that BnaC.Tic40 participates in specific protein translocation across the inner envelope membrane in the tapetal plastid, which is required for tapetal development and function...
  49. pmc Genes involved in Cronobacter sakazakii biofilm formation
    Isabel Hartmann
    Institute for Food Safety and Hygiene, Winterthurerestrasse 272, 8057 Zurich, Switzerland
    Appl Environ Microbiol 76:2251-61. 2010
    ..Our findings suggest that flagella and hypothetical proteins ESA_00281 and ESA_00282, but not cellulose, contribute to adhesion of Cronobacter to this biotic surface...
  50. pmc NS3 helicase domains involved in infectious intracellular hepatitis C virus particle assembly
    Yinghong Ma
    Center for Hepatitis Research, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555 1073, USA
    J Virol 82:7624-39. 2008
    ..These data reveal a previously unsuspected role for the NS3 helicase in early virion morphogenesis and provide a new perspective on HCV assembly...
  51. pmc Identification of the genetic determinants of Salmonella enterica serotype Typhimurium that may regulate the expression of the type 1 fimbriae in response to solid agar and static broth culture conditions
    Yin Ching Chuang
    Department of Medical Research, Chi Mei Medical Center, 901 Chung Hwa Road, Yong Kang City, Tainan 710, Taiwan
    BMC Microbiol 8:126. 2008
    ..Typhimurium is the result of the interaction and cooperation of several genes in the fim gene cluster. Other gene products that may also participate in the regulation of type 1 fimbrial expression remain uncharacterized...
  52. ncbi Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit
    Constanze Pinske
    Institute for Microbiology, Martin Luther University Halle Wittenberg, Kurt Mothes Str 3, 06120, Halle, Saale, Germany
    Arch Microbiol 193:893-903. 2011
    ..These findings demonstrate that hydrogenase-dependent reduction of BV requires the small subunit...
  53. pmc One of the two genes encoding nucleoid-associated HU proteins in Streptomyces coelicolor is developmentally regulated and specifically involved in spore maturation
    Paola Salerno
    Department of Cell and Organism Biology, Lund University, Sölvegatan 35, 22362 Lund, Sweden
    J Bacteriol 191:6489-500. 2009
    ..Overall, the results show that HupS is specifically involved in sporulation and may affect nucleoid architecture and protection in spores of S. coelicolor...
  54. pmc Fungal adenylyl cyclase integrates CO2 sensing with cAMP signaling and virulence
    Torsten Klengel
    Department of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom
    Curr Biol 15:2021-6. 2005
    ..Novel therapeutic agents could target this pathway at several levels to control fungal infections...
  55. ncbi The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp
    Tsutomu Furukawa
    Genetic Diversity Department, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan
    Plant J 49:91-102. 2007
    ..Comparison of the Rc locus among rice accessions showed that a 14-bp deletion occurred only in the rc locus...
  56. ncbi Functional characterization of NRAMP3 and NRAMP4 from the metal hyperaccumulator Thlaspi caerulescens
    Ronald J F J Oomen
    Institut des Sciences du Vegetal, CNRS, Avenue de la Terrasse, Gif sur Yvette, France
    New Phytol 181:637-50. 2009
    ..thaliana. Our data indicate that the difference between TcNRAMP3 and TcNRAMP4 and their A. thaliana orthologues does not lie in a different protein function, but probably resides in a different expression level or expression pattern...
  57. ncbi The velvet gene, FgVe1, affects fungal development and positively regulates trichothecene biosynthesis and pathogenicity in Fusarium graminearum
    Jawad Merhej
    INRA, UR1264 MycSA, 71, Avenue Edouard Bourleaux, BP81, F 33883 Villenave d Ornon, France
    Mol Plant Pathol 13:363-74. 2012
    ..Pathogenicity studies performed on flowering wheat plants indicate that FgVe1 is a positive regulator of virulence in F. graminearum...
  58. ncbi The chloroplast protein RPH1 plays a role in the immune response of Arabidopsis to Phytophthora brassicae
    Khaoula Belhaj
    Department of Biology, University of Fribourg, Fribourg, Switzerland
    Plant J 58:287-98. 2009
    ..The disease resistance-related role of RPH1 was not limited to the Arabidopsis model system. Silencing of the potato homolog StRPH1 in a resistant potato cultivar caused susceptibility to the late blight pathogen Phytophthora infestans...
  59. pmc Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype
    Yi Jiun Pan
    Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan
    J Clin Microbiol 46:2231-40. 2008
    ..We conclude that sequences from these two cps regions are very useful in detecting K57 and the new cps genotype...
  60. ncbi TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis
    Satoshi Kitamura
    Department of Ion Beam Applied Biology, Japan Atomic Energy Research Institute, 1233 Watanuki machi, Takasaki, Gunma 370 1292, Japan
    Plant J 37:104-14. 2004
    ..As involvement of GST in the PA pathway was previously considered unlikely, the function of TT19 in the PA pathway is also discussed in the context of the putative transporter for PA precursors...
  61. pmc Regulation of Cre recombinase by ligand-induced complementation of inactive fragments
    Nicolas Jullien
    ICNE UMR 6544, CNRS Université de la Méditerranée, IFR Jean Roche, Faculte de Medecine Nord, 13916 Marseille Cedex 20, France
    Nucleic Acids Res 31:e131. 2003
    ..02 nM. Thus, ligand-induced dimerization can efficiently regulate Cre, and should be useful to achieve a tight temporal control of its activity, such as in the case of the creation of conditional knock-out animals...
  62. pmc Divergent roles in Arabidopsis thaliana development and defense of two homologous genes, aberrant growth and death2 and AGD2-LIKE DEFENSE RESPONSE PROTEIN1, encoding novel aminotransferases
    Jong Tae Song
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637
    Plant Cell 16:353-66. 2004
    ..We suggest that AGD2 synthesizes an important amino acid-derived molecule that promotes development and suppresses defenses, whereas ALD1 generates a related amino acid-derived molecule important for activating defense signaling...
  63. ncbi Leptosphaeria maculans avirulence gene AvrLm4-7 confers a dual recognition specificity by the Rlm4 and Rlm7 resistance genes of oilseed rape, and circumvents Rlm4-mediated recognition through a single amino acid change
    Francis Parlange
    Biogemma, Laboratoire de Biologie Cellulaire et Moleculaire, Domaine de Sandreau, F 31700 Mondonville, France
    Mol Microbiol 71:851-63. 2009
    ....
  64. ncbi Cloning of Trypanosoma brucei and Leishmania major genes encoding the GlcNAc-phosphatidylinositol de-N-acetylase of glycosylphosphatidylinositol biosynthesis that is essential to the African sleeping sickness parasite
    Tunhan Chang
    Division of Biological Chemistry and Molecular Microbiology, The Wellcome Trust Biocentre, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 277:50176-82. 2002
    ..These results suggest that the stabilities of other glycosylphosphatidylinositol pathway enzymes are not dependent on GlcNAc-PI de-N-acetylase levels...
  65. pmc RpoH(II) activates oxidative-stress defense systems and is controlled by RpoE in the singlet oxygen-dependent response in Rhodobacter sphaeroides
    Aaron M Nuss
    Institut fur Mikrobiologie und Molekularbiologie, Heinrich Buff Ring 26, 35392 Giessen, Germany
    J Bacteriol 191:220-30. 2009
    ..Therefore, we conclude that systems pivotal for the organism's defense against photooxidative stress are strongly dependent on GSH and are specifically recognized by RpoH(II) in R. sphaeroides...
  66. ncbi Functional diversification of closely related ARF-GEFs in protein secretion and recycling
    Sandra Richter
    ZMBP, Entwicklungsgenetik, Tubingen, Germany
    Nature 448:488-92. 2007
    ....
  67. ncbi Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings
    Haruhiko Inoue
    Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Tokyo 113 8657, Japan
    J Biol Chem 284:3470-9. 2009
    ..These results demonstrate that rice OsYSL15 plays a crucial role in iron homeostasis during the early stages of growth...
  68. pmc Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe-2S] clusters
    Sibali Bandyopadhyay
    Department of Chemistry, Centre for Metalloenzyme Studies, University of Georgia, Athens, GA, USA
    EMBO J 27:1122-33. 2008
    ..Alternatively, they may function in the storage and/or delivery of preformed Fe-S clusters or in the regulation of the chloroplastic Fe-S cluster assembly machinery...
  69. pmc A Serratia marcescens OxyR homolog mediates surface attachment and biofilm formation
    Robert M Q Shanks
    Charles T Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, UPMC Eye Center, Pittsburgh, PA 15213, USA
    J Bacteriol 189:7262-72. 2007
    ..Together, these data support a model in which OxyR contributes to early stages of S. marcescens biofilm formation by influencing fimbrial gene expression...
  70. ncbi iPS cells produce viable mice through tetraploid complementation
    Xiao yang Zhao
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Nature 461:86-90. 2009
    ..We demonstrate the practicality of using iPS cells as useful tools for the characterization of cellular reprogramming and developmental potency, and confirm that iPS cells can attain true pluripotency that is similar to that of ES cells...
  71. pmc Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis
    R Watanabe
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochem J 339:185-92. 1999
    ..The disruption of the gene caused lethality in S. cerevisiae. These results indicate that GlcNAc-PI de-N-acetylase is conserved between mammals and yeasts and that the de-N-acetylation step is also indispensable in yeasts...
  72. pmc Regulation of morphogenesis and biocontrol properties in Trichoderma virens by a VELVET protein, Vel1
    Prasun K Mukherjee
    Department of Plant Pathology and Microbiology, Texas A and M University, College Station, TX 77843, USA
    Appl Environ Microbiol 76:2345-52. 2010
    ..These findings provide an opportunity for genetic enhancement of biocontrol and industrial strains of Trichoderma, since Vel1 is very highly conserved across three Trichoderma species...
  73. pmc Vibrio cholerae RND family efflux systems are required for antimicrobial resistance, optimal virulence factor production, and colonization of the infant mouse small intestine
    Xiaowen R Bina
    Department of Molecular Sciences, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, TN 38163, USA
    Infect Immun 76:3595-605. 2008
    ..Our findings show that the V. cholerae RND efflux systems are required for antimicrobial resistance, optimal virulence factor production, and colonization of the infant mouse...
  74. pmc Reintroduction of two deleted virulence loci restores full virulence to the live vaccine strain of Francisella tularensis
    Emelie Salomonsson
    FOI Swedish Defence Research Agency, Division of CBRN Defence and Security, Umea, Sweden
    Infect Immun 77:3424-31. 2009
    ..The identification of the two attenuating deletion events could facilitate the licensing of LVS for use in humans...
  75. ncbi Construction of a lipopolysaccharide reporter cell line and its use in identifying mutants defective in endotoxin, but not TNF-alpha, signal transduction
    R L Delude
    Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    J Immunol 161:3001-9. 1998
    ..Identification of the genes affected in these mutant reporter cells should identify heretofore-elusive components of the LPS signaling cascade...
  76. pmc Systematic genome instability screens in yeast and their potential relevance to cancer
    Karen W Y Yuen
    Michael Smith Laboratories, Department of Medical Genetics, 301 2185 East Mall, University of British Columbia, Vancouver, BC, Canada V6T 1Z4
    Proc Natl Acad Sci U S A 104:3925-30. 2007
    ....
  77. ncbi A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export
    J D York
    Department of Pharmacology, Duke University Medical Center, DUMC 3813, Durham, NC 27710, USA
    Science 285:96-100. 1999
    ..The common downstream effects of mutations in each component were deficiencies in IP6 synthesis and messenger RNA export, indicating a role for IP6 in GLE1 function and messenger RNA export...
  78. ncbi The poplar GT8E and GT8F glycosyltransferases are functional orthologs of Arabidopsis PARVUS involved in glucuronoxylan biosynthesis
    Chanhui Lee
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Plant Cell Physiol 50:1982-7. 2009
    ..These findings suggest that PoGT8E and PoGT8F are involved in glucuronoxylan biosynthesis during wood formation in poplar...
  79. pmc An allelic exchange system for compliant genetic manipulation of the select agents Burkholderia pseudomallei and Burkholderia mallei
    Mohamad A Hamad
    Department of Microbiology, University of Colorado School of Medicine, Mail Stop 8333, PO Box 6511, Aurora, CO 80045, USA
    Gene 430:123-31. 2009
    ..pseudomallei, and one mutant in B. mallei. This vector significantly expands the number of available tools that are select-agent compliant for the genetic manipulation of B. pseudomallei and B. mallei...
  80. pmc Hydrogen production in Chlamydomonas: photosystem II-dependent and -independent pathways differ in their requirement for starch metabolism
    Vincent Chochois
    Commissariat a l Energie Atomique, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, Commissariat à l Energie Atomique Cadarache, F 13108 Saint Paul lez Durance, France
    Plant Physiol 151:631-40. 2009
    ....
  81. pmc Suppressors of a Saccharomyces cerevisiae pkc1 mutation identify alleles of the phosphatase gene PTC1 and of a novel gene encoding a putative basic leucine zipper protein
    K N Huang
    Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Genetics 141:1275-85. 1995
    ..Because PTC1 was initially isolated as a component of the Hog1p MAPK pathway, it appears that these two MAPK cascades share a common regulatory feature...
  82. pmc Specific roles of the iroBCDEN genes in virulence of an avian pathogenic Escherichia coli O78 strain and in production of salmochelins
    Mélissa Caza
    INRS Institut Armand Frappier, 531 Boul des Prairies, Laval, Quebec, Canada H7V 1B7
    Infect Immun 76:3539-49. 2008
    ..Overall, efficient glucosylation (IroB), transport (IroC and IroN), and processing (IroD and IroE) of salmochelins are required for APEC virulence, although IroE appears to serve an ancillary role...
  83. ncbi Two duplicate CYP704B1-homologous genes BnMs1 and BnMs2 are required for pollen exine formation and tapetal development in Brassica napus
    Bin Yi
    National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, China
    Plant J 63:925-38. 2010
    ..These data suggest that BnCYP704B1 participates in a vital tapetum-specific metabolic pathway that is not only involved in exine formation but is also required for basic tapetal cell development and function...
  84. pmc Role of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis
    Paola Caro-Hernández
    Departamento de Microbiologia y Genetica, Edificio Departamental, Universidad de Salamanca, Plaza Doctores de la Reina s n, 37007 Salamanca, Spain
    Infect Immun 75:4050-61. 2007
    ..ovis inside host cells. This aspect, together with the role of Omp25d and Omp22 in the virulence both of B. ovis in rams and of other Brucella species, should be thoroughly evaluated in future studies...
  85. ncbi SDHAF1, encoding a LYR complex-II specific assembly factor, is mutated in SDH-defective infantile leukoencephalopathy
    Daniele Ghezzi
    Unit of Molecular Neurogenetics Pierfranco and Luisa Mariani Center for Study of Children s Mitochondrial Disorders, Foundation IRCCS Neurological Institute C Besta, Milan, Italy
    Nat Genet 41:654-6. 2009
    ..SDHAF1 is the first bona fide SDH assembly factor reported in any organism...
  86. pmc The pea GIGAS gene is a FLOWERING LOCUS T homolog necessary for graft-transmissible specification of flowering but not for responsiveness to photoperiod
    Valerie Hecht
    School of Plant Science, University of Tasmania, Hobart, Tasmania 7001, Australia
    Plant Cell 23:147-61. 2011
    ..These results suggest that induction of flowering by photoperiod in pea results from interactions among several members of a diversified FT family...
  87. ncbi The effector protein Avr2 of the xylem-colonizing fungus Fusarium oxysporum activates the tomato resistance protein I-2 intracellularly
    Petra M Houterman
    Plant Pathology, Swammerdam Institute for Life Sciences, University of Amsterdam, PO Box 94062, 1090 GB Amsterdam, The Netherlands
    Plant J 58:970-8. 2009
    ..Although Avr2 is secreted into the xylem sap when Fol colonizes tomato, the Avr2 protein can be recognized intracellularly by I-2, implying uptake by host cells...
  88. pmc Reconstitution and subunit geometry of human condensin complexes
    Itay Onn
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    EMBO J 26:1024-34. 2007
    ..Cleavage pattern of SMC2 by limited proteolysis is changed upon its binding to ATP or DNA. Our results shed new light on the architecture and dynamics of this highly elaborate machinery designed for chromosome assembly...
  89. pmc Characterization and analysis of the PikD regulatory factor in the pikromycin biosynthetic pathway of Streptomyces venezuelae
    D J Wilson
    Department of Microbiology and Biological Process Technology Institute, University of Minnesota, 420 Delaware Street S.E, Minneapolis, MN 55455, USA
    J Bacteriol 183:3468-75. 2001
    ..venezuelae wild-type and pikD mutant strains. This analysis demonstrated that PikD-mediated transcriptional regulation occurs at promoters controlling expression of pikRII, pikAI, and desI but not those controlling pikRI or pikC...
  90. ncbi AtHMA1 contributes to the detoxification of excess Zn(II) in Arabidopsis
    Yu Young Kim
    POSTECH UZH Global Research Laboratory, Division of Molecular Life Sciences, Pohang University of Science and Technology, Pohang, Korea
    Plant J 58:737-53. 2009
    ..Taken together, these results suggest that under conditions of excess Zn(II), AtHMA1 contributes to Zn(II) detoxification by reducing the Zn content of Arabidopsis thaliana plastids...
  91. pmc Individual Mycobacterium tuberculosis resuscitation-promoting factor homologues are dispensable for growth in vitro and in vivo
    JoAnn M Tufariello
    Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Infect Immun 72:515-26. 2004
    ..tuberculosis rpf genes, while overlapping to various degrees, are not uniform. We also provide evidence that mycobacterial rpf genes are expressed in vivo in the lungs of mice acutely infected with virulent M. tuberculosis...
  92. pmc Mutations of an alpha1,6 mannosyltransferase inhibit endoplasmic reticulum-associated degradation of defective brassinosteroid receptors in Arabidopsis
    Zhi Hong
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, An Arbor, Michigan 48109 1048, USA
    Plant Cell 21:3792-802. 2009
    ..Our results strongly suggest that the complete assembly of the lipid-linked glycans is essential for successful quality control of defective glycoproteins in Arabidopsis...
  93. pmc Cytochrome P450 family member CYP704B2 catalyzes the {omega}-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice
    Hui Li
    School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China
    Plant Cell 22:173-90. 2010
    ....
  94. pmc Arabidopsis synaptotagmin 1 is required for the maintenance of plasma membrane integrity and cell viability
    Arnaldo L Schapire
    Departamento de Biologia Molecular y Bioquimica, Universidad de Malaga, 29071 Malaga, Spain
    Plant Cell 20:3374-88. 2008
    ..Our analyses support that membrane trafficking mediated by SYT1 is important for plasma membrane integrity and plant fitness...
  95. ncbi MgCRZ1, a transcription factor of Magnaporthe grisea, controls growth, development and is involved in full virulence
    Haifeng Zhang
    Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Ministry of Agriculture, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Nanjing, China
    FEMS Microbiol Lett 293:160-9. 2009
    ..We conclude that MgCRZ1 is essential for growth, development, and full virulence of M. grisea...
  96. ncbi Presence of a functional flagellar cluster Flag-2 and low-temperature expression of flagellar genes in Yersinia enterocolitica W22703
    Geraldine Bresolin
    Zentralinstitut für Ernährungs und Lebensmittelforschung ZIEL, Abteilung Mikrobiologie, Technische Universitat Munchen, Weihenstephaner Berg 3, 85354 Freising, Germany
    Microbiology 154:196-206. 2008
    ..In a motility assay, a non-motile and a hyper-motile phenotype resulted from knockout mutations of the Flag-1 genes fliS1 and fliT, respectively. Complemented strains validated these results, confirming the regulatory role of FliT...
  97. ncbi PPE and PE_PGRS proteins of Mycobacterium marinum are transported via the type VII secretion system ESX-5
    Abdallah M Abdallah
    Department of Medical Microbiology and Infection Control, VU University Medical Centre, Amsterdam, The Netherlands
    Mol Microbiol 73:329-40. 2009
    ..Together, these data show that ESX-5 is probably a major secretion pathway for mycobacteria and that this system is responsible for the secretion of recently evolved PE_PGRS and PPE proteins...
  98. pmc JMJ14, a JmjC domain protein, is required for RNA silencing and cell-to-cell movement of an RNA silencing signal in Arabidopsis
    Iain R Searle
    Plant Sciences Department, Cambridge University, Cambridge, United Kingdom
    Genes Dev 24:986-91. 2010
    ..Our results illustrate a link between RNA silencing and demethylation of histone H3 trimethylysine. We propose that JMJ14 acts downstream from the Argonaute effector complex to demethylate histone H3K4 at the target of RNA silencing...
  99. pmc Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential
    Scott A Saracco
    Department of Genetics, University of Wisconsin, Madison, WI 53706 1574, USA
    Plant Physiol 145:119-34. 2007
    ..Taken together, it appears that SIZ1-mediated conjugation of SUMO1 and SUMO2 to other intracellular proteins is essential in Arabidopsis, possibly through stress-induced modification of a potentially diverse pool of nuclear proteins...
  100. pmc Dolichol biosynthesis and its effects on the unfolded protein response and abiotic stress resistance in Arabidopsis
    Hairong Zhang
    State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
    Plant Cell 20:1879-98. 2008
    ..Our data suggest that LEW1 catalyzes dolichol biosynthesis and that dolichol is important for plant responses to endoplasmic reticulum stress, drought, and dark-induced senescence in Arabidopsis...
  101. ncbi The response of Escherichia coli to exposure to the biocide polyhexamethylene biguanide
    Michael J Allen
    School of Biosciences, Cardiff University, Museum Avenue, PO Box 911, Cardiff CF10 3US, UK
    Microbiology 152:989-1000. 2006
    ....

Research Grants61

  1. Doctoral Training/Biochemistry/Transsulfuration Enzymes
    Andre Hudson; Fiscal Year: 2005
    ..kinetic experiments, and by examining the function of the enzyme under in vivo conditions using a genetic complementation test. The physiological role of the gene product will also be explored by studying its expression, its ..
  2. Genetics of Alcohol Sensitivity in Drosophila
    Trudy Mackay; Fiscal Year: 2006
    ....
  3. OmpR and acid regulation of Escherichia coli fim genes
    WILLIAM SCHWAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  4. Proline uptake in staphylococcus aureus pathogenesis
    WILLIAM SCHWAN; Fiscal Year: 2002
    ..The results of this study will help us understand the role of proline transport in S. aureus infections. ..
  5. Archaeal gene recruitment by the Chlamydiales
    David Graham; Fiscal Year: 2008
    ..Results from these experiments will support future microbiological studies of the role of this system in chlamydial infection and survival in the host cell. [unreadable] [unreadable] [unreadable]..
  6. Herpes Simplex Virus Capsid Assembly and DNA Packaging
    FREDERICK L HOMA; Fiscal Year: 2010
    ..These studies aim to elucidate the mechanism underlying HSV DNA cleavage and packaging and may suggest novel targets for the development of antivirals. ..
  7. MECHANISMS OF GONOCOCCAL PILIN ANTIGENIC/PHASE VARIATION
    H Seifert; Fiscal Year: 2002
    ..Through these investigations, this project will continue to define the molecular mechanism used to allow these high frequency recombination reactions that are of critical importance to the pathogenesis of gonorrhea. ..
  8. Gonococcal Pilus Structure and Function
    H Seifert; Fiscal Year: 2008
    ..These studies will provide new findings and insights into the structure and functions of the gonococcal pilus. ..
  9. DEVELOPMENT OF A VACCINE FOR MORAXELLA CATARRHALIS
    Timothy Murphy; Fiscal Year: 2009
    ..catarrhalis surface, the identification of surface antigens expressed specifically during human infection and critical information about the human immune response to surface antigens of M. catarrhalis. ..
  10. Screening for Novel Modulators of Notch/RBPjk Interactions
    MA XENIA ILAGAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  11. Development of Novel Antiviral Agents VS West Nile Virus
    Michael Diamond; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  12. Functions of NS3 in HCV RNA Replication
    Minkyung Yi; Fiscal Year: 2006
    ..abstract_text> ..
  13. Juvenile Hormone Receptor in Flies and Mosquitoes
    Thomas Wilson; Fiscal Year: 2007
    ..In addition, a better understanding of Met as a bHLH-PAS gene will further our knowledge of this family of transcriptional regulators, including homologous genes found in humans. [unreadable] [unreadable]..
  14. The role of the CCAAT-binding factor in Candida albicans
    DAVID MCNABB; Fiscal Year: 2007
    ..albicans virulence; and 3) to dissect the regulatory function of the CCAAT-binding factor. ..
  15. Congugal DNA transfer into M. tuberculosis
    KEITH DERBYSHIRE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  16. Analysis of anti-growth properties of processed Follistatin proteins
    THEODOR HAERRY; Fiscal Year: 2007
    ..The final goal of the proposed work is to engineering modified FS proteins with greater anti-growth properties that could be used to combat cancers caused by mutations in TGF-b signaling components. [unreadable] [unreadable] [unreadable]..
  17. Genetic Control of Schwann Cell Differentiation
    JOHN RUTLEDGE BERMINGHAM; Fiscal Year: 2011
    ..By understanding how the Lgi4 protein controls Schwann cell development, we will establish new avenues for the treatment of myelin disorders and nerve injury. ..
  18. MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903
    KEITH DERBYSHIRE; Fiscal Year: 2004
    ..3. To establish biochemical assays that will identify specific transposase-DNA contacts, define stable domains of the protein and facilitate the development of an in vitro transposition system. ..
  19. Summer Institute Preparation for Careers in Microbiology
    JORGE ESCALANTE; Fiscal Year: 2002
    ..The Institute is the first and only program of its kind providing students with hands-on, practical training in communications and teaching, ethics, grant preparation and review. ..
  20. Encapsidation of Adenovirus DNA
    Michael J Imperiale; Fiscal Year: 2010
    ..This knowledge will be applicable to the development of anti-viral drugs that block this process, as well as to the development of safer adenovirus vectors and recombinant vaccines. ..
  21. CONJUGATION AND RECOMBINATION IN MYCOBACTERIA
    KEITH DERBYSHIRE; Fiscal Year: 2009
    ..2. To identify and characterize trans-acting transfer functions in both donor and recipient cells. 3. To examine transfer of chromosomal and plasmid DNA among fast- and slow-growing mycobacteria. ..
  22. INTERFERON IN MODULATING DENGUE VIRUS INFECTION
    Michael Diamond; Fiscal Year: 2002
    ....
  23. CCM proteins and vascular MAPK signal transduction
    Michael Malone; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  24. RECOMBINANT ADENOVIRAL VECTORS FOR CARDIOVASCULAR DISEAS
    Michael Imperiale; Fiscal Year: 2003
    ..Together, these vector improvements will facilitate gene therapy of a range of cardiovascular diseases. ..
  25. Selenoprotein Synthesis: Redefinition of Selenocysteine-encoding UGA Codons
    Michael T Howard; Fiscal Year: 2010
    ....
  26. B12 Biosysthesis and Anaerobic Metabolism in Salmonella
    JORGE ESCALANTE; Fiscal Year: 2009
    ..Lastly, we will begin to dissect the biosynthetic pathways for the lower ligand base. One pathway is largely non-enzymatic while the other one is a piecemeal pathway. ..
  27. Genetics of coordinately evolving patterns and behavior
    John R True; Fiscal Year: 2010
    ....
  28. Control of virulence in Neisseria meningitidis
    Yih Ling Tzeng; Fiscal Year: 2009
    ..meningitidis and are important to the development of new approaches for meningococcal disease prevention and treatment. ..
  29. Cellular & Molecular Fungal Biology 2008 Gordon Research Conference
    Nancy Keller; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  30. GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLI
    Valley J Stewart; Fiscal Year: 2010
    ..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
  31. QTL mapping age-related changes in lipid storage
    Maria De Luca; Fiscal Year: 2007
    ..Moreover, knowledge of the nature and frequency of causative genetic polymorphisms will lead to a better understanding of the mechanisms that maintain genetic variation in these traits in natural populations. ..
  32. Antisense Mediated Suppression of Dystrophin Mutations
    Michael Howard; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  33. Studies of behavioral quiescence in C. elegans
    David Raizen; Fiscal Year: 2008
    ..Raizen while he learns the scientific field of sleep and chronobiology and develops C. elegans as a model system for the study of behavioral quiescence and its relationship to development. ..
  34. BK Virus as a Co-Factor in Prostate Cancer
    Michael Imperiale; Fiscal Year: 2009
    ..If a role for BKV in prostate cancer exists, there is the possibility of developing therapeutics or vaccines that are specific for the virus, thereby reducing the morbidity, mortality, and even the incidence of this cancer. ..
  35. New genetic approaches analyzing Borrelia burgdorferi
    Jon Blevins; Fiscal Year: 2007
    ..This proposal thus seeks to contribute major new advances to borrelial genetics and to use those developments to examine potentially novel aspects of Bb virulence expression. [unreadable] [unreadable]..
  36. Expression of Cell Surface Proteins in Streptococcus mutans
    Indranil Biswas; Fiscal Year: 2007
    ..mutans will provide opportunities for the development of new approaches to the prevention and treatment of dental caries. [unreadable] [unreadable] [unreadable]..
  37. GENETIC ANALYSIS OF ENZYME PROCESSING AND LOCALIZATION
    Elizabeth Jones; Fiscal Year: 2002
    ..Methods will include targeted mutagenesis, suppressor selection and analysis, subcellular fractionation, biochemistry, and immunofluorescence. ..
  38. Recurrent otitis media and COPD: immunity and vaccines
    Timothy Murphy; Fiscal Year: 2004
    ..The proposed studies will advance the field of vaccine development to prevent otitis media and respiratory tract infections in COPD. ..
  39. Assembling a Centric Nucleosome
    Richard Baker; Fiscal Year: 2004
    ..Given the high conservation of centric histone H3 variants throughout evolution, the results will illuminate the problem of centromere formation and function in higher eukaryotes including humans. ..
  40. POSTTRANSCRIPTIONAL REGULATION OF VIRAL MRNA SYNTHESIS
    Michael Imperiale; Fiscal Year: 2001
    ..Third, the reason for expression of the 52/55 kDa protein during the early phase of the infection will be examined. Together, these studies address the how and why of regulation of expression of L1 mRNAs during infection. ..
  41. Recombinant Adenovirus Vaccines Against B. anthracis
    Michael Imperiale; Fiscal Year: 2006
    ..anthracis vaccine, and will establish a platform for similar vaccines against other infectious agents that might be used by bioterrorists. ..
  42. GENETIC ANALYSIS OF GBS ADHERENCE TO FIBRONECTIN
    GLEN TAMURA; Fiscal Year: 2002
    ..These studies will allow for identification of the structural gene(s) for fibronectin adhesin(s). ..
  43. Imaging Vaccina Viral-Host Pathogenesis
    Gary Luker; Fiscal Year: 2007
    ..Collectively, the research is expected to facilitate the development of improved vaccines and treatments for smallpox, thereby enhancing our ability to prevent the use of smallpox for bioterrorism. [unreadable] [unreadable] [unreadable]..
  44. Peptide Inhibitors of HIV-1 Vif Function
    John Donahue; Fiscal Year: 2007
    ..This research is relevant to public health because it may lead to the development of new therapeutic agents for the treatment of HIV/AIDS. [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  45. RECOMBINATION MECHANISMS IN YEAST CELL DIFFERENTIATION
    James Haber; Fiscal Year: 2006
    ..abstract_text> ..
  46. Characterization of a novel TGF-beta signaling component
    THEODOR HAERRY; Fiscal Year: 2004
    ..This project has great potential to contribute new insights into the mechanism of TGF-Beta signaling and our understanding of the development of certain cancers. It may also provide a new target to combat these diseases. ..
  47. RNA Replicons to Enhance Nasal Vaccines
    CASEY MORROW; Fiscal Year: 2004
    ..abstract_text> ..
  48. Molecular Complementation of Nuclear Receptors
    John T Koh; Fiscal Year: 2010
    ..We will further develop these "functionally orthogonal" estrogen/ER pairs as novel chemical biology tools for dissecting the seven or more estrogen dependent signaling pathways. ..
  49. GENETIC VARIATION IN MURINE LONG BONE GROWTH AND DEVELOPMENT
    James M Cheverud; Fiscal Year: 2010
    ..The outcome of these experiments will be a better understanding of the genetic, molecular, and cellular processes responsible for variation in limb growth with consequences for our understanding of pathologies of growth. ..
  50. Genetic Environmental Relationship Between Bone Obesity and Leptin in Mice
    James M Cheverud; Fiscal Year: 2010
    ..This project will identify novel genes and physiological pathways affecting osteoporosis and examine how genetic and environmentally-based obesity affects bone mass, morphology and biomechanical function. ..
  51. Light-regulated genes affecting the pathogen potential of Cryptococcus neoformans
    Alexander Idnurm; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  52. A Mouse Model for Complex Human Diseases
    James Cheverud; Fiscal Year: 2009
    ..Backcrossing with SM/J will be used to narrow the QTL interval for strain loss and to produce a new strain, carrying the unusual phenotypes of SM/J without requiring enforced heterozygosity at the agouti locus. ..
  53. GENE INTERACTION FOR BODY WEIGHT COMPONENTS IN MICE
    James Cheverud; Fiscal Year: 2002
    ..In addition to positional cloning of the QTLs, congenic strains will be formed for each QTL and will be tested, along with the LGxSM recombinant inbred strains for a variety of obesity-related traits. ..
  54. Genetics Basis of Dietary Obesity in Mice
    James Cheverud; Fiscal Year: 2009
    ..These studies will increase our knowledge of the important physiological effects of dietary fat, including the genetic variants responsible for individual variations in response to a high fat diet. ..
  55. Airway Inflammation: Bacterial Epithelial Interactions
    BRYAN HURLEY; Fiscal Year: 2004
    ..Results obtained from the following proposal may shed light on diseases involving bacterial mediated lung inflammation such as cystic fibrosis and should also provide new strategies directed towards treating lung disease. ..
  56. MOLECULAR BASIS AND ENZYMOLOGY OF MICROBIAL BIOSYNTHESIS
    F Tabita; Fiscal Year: 2003
    ..For each enzyme, recombinant systems will be used to facilitate efforts to learn how these proteins function specifically in biosynthesis and how control of their activity influences normal cellular metabolism. ..
  57. GONOCOCCI: GENETICS OF RESISTANCE TO PMN PROTEINS
    William Shafer; Fiscal Year: 2009
    ..abstract_text> ..
  58. Hemin Receptor Gene Family of Bartonella quintana
    MICHAEL MINNICK; Fiscal Year: 2006
    ..abstract_text> ..
  59. Biochemical and Genetic Studies of Latent Membrane Pro 2
    Richard Longnecker; Fiscal Year: 2007
    ..3) Investigate the importance of ubiquitination and role of Nedd4-family ubiqutin ligases in LMP2A function. 4) Investigate the properties of LCLs transformed and latently infected with the LMP2A mutant viruses. ..
  60. Replication Elements in the 5' End of the HCV Genome
    Minkyung Yi; Fiscal Year: 2004
    ..abstract_text> ..