genetic engineering

Summary

Summary: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.

Top Publications

  1. ncbi Enzymatic assembly of DNA molecules up to several hundred kilobases
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
    Nat Methods 6:343-5. 2009
  2. pmc Genetic engineering of human pluripotent cells using TALE nucleases
    Dirk Hockemeyer
    The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
    Nat Biotechnol 29:731-4. 2011
  3. pmc Simple and highly efficient BAC recombineering using galK selection
    Søren Warming
    Mouse Cancer Genetics Program, National Cancer Institute Frederick, MD 21702 1201, USA
    Nucleic Acids Res 33:e36. 2005
  4. pmc Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases
    Elena E Perez
    Abramson Family Cancer Research Institute, Department of Pathology and Laboratory Medicine, 421 Curie Blvd, Room 554, BRB II III, Philadelphia, Pennsylvania 19104 6160, USA
    Nat Biotechnol 26:808-16. 2008
  5. ncbi Creation of a bacterial cell controlled by a chemically synthesized genome
    Daniel G Gibson
    The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 329:52-6. 2010
  6. ncbi Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
    Mamie Z Li
    Howard Hughes Medical Institute, Department of Genetics, Harvard Partners Center for Genetics and Genomics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Methods 4:251-6. 2007
  7. pmc Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli
    Parayil Kumaran Ajikumar
    Department of Chemical Engineering, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA
    Science 330:70-4. 2010
  8. pmc Automated design of synthetic ribosome binding sites to control protein expression
    Howard M Salis
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA
    Nat Biotechnol 27:946-50. 2009
  9. pmc Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
    Yannick Doyon
    Sangamo BioSciences, Inc, Pt Richmond Tech Center, 501 Canal Blvd, Suite A100, Richmond, California 94804, USA
    Nat Biotechnol 26:702-8. 2008
  10. pmc Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
    Xiangdong Meng
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 6th Floor, 364 Plantation St, Worcester, Massachusetts 01605, USA
    Nat Biotechnol 26:695-701. 2008

Research Grants

Detail Information

Publications259 found, 100 shown here

  1. ncbi Enzymatic assembly of DNA molecules up to several hundred kilobases
    Daniel G Gibson
    The J Craig Venter Institute, Synthetic Biology Group, Rockville, Maryland, USA
    Nat Methods 6:343-5. 2009
    ..This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool...
  2. pmc Genetic engineering of human pluripotent cells using TALE nucleases
    Dirk Hockemeyer
    The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
    Nat Biotechnol 29:731-4. 2011
    Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases...
  3. pmc Simple and highly efficient BAC recombineering using galK selection
    Søren Warming
    Mouse Cancer Genetics Program, National Cancer Institute Frederick, MD 21702 1201, USA
    Nucleic Acids Res 33:e36. 2005
    ....
  4. pmc Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases
    Elena E Perez
    Abramson Family Cancer Research Institute, Department of Pathology and Laboratory Medicine, 421 Curie Blvd, Room 554, BRB II III, Philadelphia, Pennsylvania 19104 6160, USA
    Nat Biotechnol 26:808-16. 2008
    ..Thus adoptive transfer of ex vivo expanded CCR5 ZFN-modified autologous CD4(+) T cells in HIV patients is an attractive approach for the treatment of HIV-1 infection...
  5. ncbi Creation of a bacterial cell controlled by a chemically synthesized genome
    Daniel G Gibson
    The J Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA
    Science 329:52-6. 2010
    ..The new cells have expected phenotypic properties and are capable of continuous self-replication...
  6. ncbi Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
    Mamie Z Li
    Howard Hughes Medical Institute, Department of Genetics, Harvard Partners Center for Genetics and Genomics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Methods 4:251-6. 2007
    ..This flexibility allows much greater versatility in the generation of recombinant DNA for the purposes of synthetic biology...
  7. pmc Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli
    Parayil Kumaran Ajikumar
    Department of Chemical Engineering, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA
    Science 330:70-4. 2010
    ..More broadly, the modular pathway engineering approach helped to unlock the potential of the MEP pathway for the engineered production of terpenoid natural products...
  8. pmc Automated design of synthetic ribosome binding sites to control protein expression
    Howard M Salis
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA
    Nat Biotechnol 27:946-50. 2009
    ..The proposed forward engineering approach should accelerate the construction and systematic optimization of large genetic systems...
  9. pmc Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
    Yannick Doyon
    Sangamo BioSciences, Inc, Pt Richmond Tech Center, 501 Canal Blvd, Suite A100, Richmond, California 94804, USA
    Nat Biotechnol 26:702-8. 2008
    ..The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg...
  10. pmc Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
    Xiangdong Meng
    Program in Gene Function and Expression, University of Massachusetts Medical School, Lazare Research Building, 6th Floor, 364 Plantation St, Worcester, Massachusetts 01605, USA
    Nat Biotechnol 26:695-701. 2008
    ..The use of engineered ZFNs to introduce heritable mutations into a genome obviates the need for embryonic stem cell lines and should be applicable to most animal species for which early-stage embryos are easily accessible...
  11. ncbi Consolidated bioprocessing of cellulosic biomass: an update
    Lee R Lynd
    Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA
    Curr Opin Biotechnol 16:577-83. 2005
    ..Recent studies of the fundamental principles of microbial cellulose utilization support the feasibility of CBP...
  12. pmc An efficient recombination system for chromosome engineering in Escherichia coli
    D Yu
    Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 97:5978-83. 2000
    ..This system will be especially useful for the engineering of large bacterial plasmids such as those from bacterial artificial chromosome libraries...
  13. ncbi Refinement and standardization of synthetic biological parts and devices
    Barry Canton
    Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, 68 580, Cambridge, Massachusetts 02139, USA
    Nat Biotechnol 26:787-93. 2008
    ..The process of refinement and characterization leading to the BBa_F2620 datasheet may serve as a starting template for producing many standardized genetically encoded objects...
  14. ncbi Production of the antimalarial drug precursor artemisinic acid in engineered yeast
    Dae Kyun Ro
    California Institute of Quantitative Biomedical Research, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
    Nature 440:940-3. 2006
    ..annua, yield optimization and industrial scale-up will be required to raise artemisinic acid production to a level high enough to reduce artemisinin combination therapies to significantly below their current prices...
  15. ncbi Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
    Farren J Isaacs
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 333:348-53. 2011
    ..Our methods treat the chromosome as both an editable and an evolvable template, permitting the exploration of vast genetic landscapes...
  16. ncbi Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance
    Wangxia Wang
    The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, and the Otto Warburg Center for Agricultural Biotechnology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
    Planta 218:1-14. 2003
    ..The review examines the following aspects: regulatory controls, metabolite engineering, ion transport, antioxidants and detoxification, late embryogenesis abundant (LEA) and heat-shock proteins...
  17. pmc A highly efficient recombineering-based method for generating conditional knockout mutations
    Pentao Liu
    Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA
    Genome Res 13:476-84. 2003
    ..This method should also facilitate the generation of knock-in mutations and transgene constructs, as well as expedite the analysis of regulatory elements and functional domains in or near genes...
  18. ncbi Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
    Shota Atsumi
    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, 420 Westwood Plaza, Los Angeles, California 90095, USA
    Nature 451:86-9. 2008
    ..In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation...
  19. pmc Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification
    Morgan L Maeder
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Cell 31:294-301. 2008
    ..In summary, OPEN provides an "open-source" method for rapidly engineering highly active zinc-finger arrays, thereby enabling broader practice, development, and application of ZFN technology for biological research and gene therapy...
  20. ncbi Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
    Eric J Steen
    Joint BioEnergy Institute, Emeryville, California 94608, USA
    Nature 463:559-62. 2010
    ..Furthermore, we show engineering of the biodiesel-producing cells to express hemicellulases, a step towards producing these compounds directly from hemicellulose, a major component of plant-derived biomass...
  21. ncbi TALEN-mediated precise genome modification by homologous recombination in zebrafish
    Yao Zu
    Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, College of Life Sciences, Peking University, Beijing, China
    Nat Methods 10:329-31. 2013
    ..We also successfully targeted two additional genes. Homologous recombination in zebrafish with a dsDNA donor expands the utility of this model organism...
  22. ncbi Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery
    Angelo Lombardo
    San Raffaele Telethon Institute for Gene Therapy, Via Olgettina, 58, 20132 Milan, Italy
    Nat Biotechnol 25:1298-306. 2007
    ....
  23. pmc A phage integrase directs efficient site-specific integration in human cells
    A C Groth
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    Proc Natl Acad Sci U S A 97:5995-6000. 2000
    ..These observations form the basis for site-specific integration strategies potentially useful in a broad range of genetic engineering applications.
  24. pmc Recombineering: a homologous recombination-based method of genetic engineering
    Shyam K Sharan
    Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    Nat Protoc 4:206-23. 2009
    Recombineering is an efficient method of in vivo genetic engineering applicable to chromosomal as well as episomal replicons in Escherichia coli. This method circumvents the need for most standard in vitro cloning techniques...
  25. ncbi Synthetic biology moving into the clinic
    Warren C Ruder
    Howard Hughes Medical Institute, Department of Biomedical Engineering, and Center for Biodynamics, Boston University, Boston, MA 02115, USA
    Science 333:1248-52. 2011
    ..Here, we highlight advances in the biomedical application of synthetic biology and discuss the field's clinical potential...
  26. ncbi Challenges in engineering microbes for biofuels production
    Gregory Stephanopoulos
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 315:801-4. 2007
    ..There is justified optimism that the full potential of biofuel production from cellulosic biomass will be obtainable in the next 10 to 15 years...
  27. ncbi Emergent properties of reduced-genome Escherichia coli
    György Pósfai
    Institute of Biochemistry, Biological Research Center, H 6726 Szeged, Hungary
    Science 312:1044-6. 2006
    ..Eradication of stress-induced transposition evidently stabilized the MDS genomes and provided some of the new properties...
  28. doi Unexpected failure rates for modular assembly of engineered zinc fingers
    Cherie L Ramirez
    Nat Methods 5:374-5. 2008
  29. pmc Targeted chromosomal deletions in human cells using zinc finger nucleases
    Hyung Joo Lee
    Department of Chemistry, Seoul National University, Seoul, South Korea
    Genome Res 20:81-9. 2010
    ..Thus, ZFN-induced genomic deletions should be broadly useful as a novel method in biomedical research, biotechnology, and gene therapy...
  30. pmc Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification
    Barry L Stoddard
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, A3 025, Seattle, WA 98109, USA
    Structure 19:7-15. 2011
    ..Engineered homing endonucleases proteins are now being used to generate targeted genomic modifications for a variety of biotech and medical applications...
  31. ncbi Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
    B Karsten Tischer
    Cornell University, Ithaca, NY, USA
    Biotechniques 40:191-7. 2006
    ....
  32. pmc A synchronized quorum of genetic clocks
    Tal Danino
    Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA
    Nature 463:326-30. 2010
    ..Furthermore, it provides a specific model system for the generation of a mechanistic description of emergent coordinated behaviour at the colony level...
  33. ncbi Serine recombinases as tools for genome engineering
    William R A Brown
    School of Biology, Queens Medical Centre, Nottingham NG7 2UH, UK
    Methods 53:372-9. 2011
    ....
  34. pmc Tuning genetic control through promoter engineering
    Hal Alper
    Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56 469, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 102:12678-83. 2005
    ....
  35. pmc Versatile RNA-sensing transcriptional regulators for engineering genetic networks
    Julius B Lucks
    Department of Bioengineering, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 108:8617-22. 2011
    ..The combination of these three features within a single RNA-based regulatory mechanism has the potential to simplify the design and construction of genetic networks by directly propagating signals as RNA molecules...
  36. pmc BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals
    Ina Poser
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Nat Methods 5:409-15. 2008
    ..The same high-throughput approach will be generally applicable to other model systems...
  37. pmc Progress in metabolic engineering of Saccharomyces cerevisiae
    Elke Nevoigt
    Department of Microbiology and Genetics, Berlin University of Technology, Seestr 13, 13353 Berlin, Germany
    Microbiol Mol Biol Rev 72:379-412. 2008
    ..resulted in substantial accumulated knowledge concerning genetics, physiology, and biochemistry as well as genetic engineering and fermentation technologies. S...
  38. pmc Cancer regression in patients after transfer of genetically engineered lymphocytes
    Richard A Morgan
    Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    Science 314:126-9. 2006
    ..This study suggests the therapeutic potential of genetically engineered cells for the biologic therapy of cancer...
  39. ncbi Engineering stability in gene networks by autoregulation
    A Becskei
    EMBL, Structures and Biocomputing, Heidelberg, Germany
    Nature 405:590-3. 2000
    ..Here we have designed and constructed simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli and we show the gain of stability produced by negative feedback...
  40. ncbi Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis
    D M McCarty
    UNC Gene Therapy Center, University of North Carolina at Chapel Hill, NC, USA
    Gene Ther 8:1248-54. 2001
    ..These novel scAAV vectors represent a biochemical intermediate in rAAV transduction and should provide new insights into the biology of vector transduction...
  41. pmc Microbial cellulose utilization: fundamentals and biotechnology
    Lee R Lynd
    Chemical and Biochemical Engineering, Thayer School of Engineering and Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Microbiol Mol Biol Rev 66:506-77, table of contents. 2002
    ....
  42. pmc Eradication of B-lineage cells and regression of lymphoma in a patient treated with autologous T cells genetically engineered to recognize CD19
    James N Kochenderfer
    Surgery Branch, National Cancer Institute, Bethesda, MD 20892, USA
    Blood 116:4099-102. 2010
    ..Adoptive transfer of anti-CD19-CAR-expressing T cells is a promising new approach for treating B-cell malignancies. This study is registered at www.clinicaltrials.gov as #NCT00924326...
  43. pmc Genome-scale promoter engineering by coselection MAGE
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA
    Nat Methods 9:591-3. 2012
    ..Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks...
  44. pmc Next-generation synthetic gene networks
    Timothy K Lu
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 27:1139-50. 2009
    ..As these challenges are addressed, synthetic biologists will be able to construct useful next-generation synthetic gene networks with real-world applications in medicine, biotechnology, bioremediation and bioenergy...
  45. ncbi Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
    Vincent J J Martin
    Department of Chemical Engineering, 201 Gilman Hall, University of California, Berkeley, California 94720 1462, USA
    Nat Biotechnol 21:796-802. 2003
    ....
  46. ncbi Genome engineering
    Peter A Carr
    Massachusetts Institute of Technology, Media Lab, Center for Bits and Atoms, Massachusetts, Cambridge, USA
    Nat Biotechnol 27:1151-62. 2009
    ..These developments promise genetic engineering with unprecedented levels of design originality and offer new avenues to expand both our understanding of ..
  47. ncbi Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives
    Raj Kumar
    Radiation Biotechnology Unit, Division of Radiation Biology and Radiation Protection, Institute of Nuclear Medicine and Allied Sciences, New Delhi, 110054 India
    J Ind Microbiol Biotechnol 35:377-91. 2008
    ....
  48. pmc Reprogramming cellular behavior with RNA controllers responsive to endogenous proteins
    Stephanie J Culler
    Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, MC 210 41, California Institute of Technology, Pasadena, CA 91125, USA
    Science 330:1251-5. 2010
    ..Our work provides a genetic platform that can build programmable sensing-actuation devices enabling autonomous control over cellular behavior...
  49. ncbi Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: current state and perspectives
    Akinori Matsushika
    Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology, Kure, Hiroshima 737 0197, Japan
    Appl Microbiol Biotechnol 84:37-53. 2009
    ..cerevisiae. Recent studies using several promising approaches such as host strain selection and adaptation to obtain further improved xylose-utilizing S. cerevisiae are also addressed...
  50. pmc Genetic engineering of algae for enhanced biofuel production
    Randor Radakovits
    Department of Chemistry and Geochemistry, Colorado School of Mines, Golden, CO 80401, USA
    Eukaryot Cell 9:486-501. 2010
    ..and other energy storage compounds in photosynthetic organisms, yeast, and bacteria through genetic engineering. Many improvements have been realized, including increased lipid and carbohydrate production, improved H(2) ..
  51. ncbi A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen
    Jason Moffat
    Broad Institute of MIT and Harvard, Cambridge, MA 02139, USA
    Cell 124:1283-98. 2006
    ..This work provides a widely applicable resource for loss-of-function screens, as well as a roadmap for its application to biological discovery...
  52. ncbi Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Plant Biotechnol J 6:392-402. 2008
    ..The generated RNAi lines were stable, viable and did not show any obvious phenotype, thus providing a robust tool for the production of therapeutically relevant glycoproteins in plants with a humanized N-glycan structure...
  53. pmc High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells
    Christopher S Raymond
    Program in Developmental Biology, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS ONE 2:e162. 2007
    ..Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and PhiC31 (PhiC31o) SSRs, which result in recombination efficiencies similar to Cre...
  54. ncbi Manufacturing molecules through metabolic engineering
    Jay D Keasling
    Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA
    Science 330:1355-8. 2010
    ..In any future, metabolic engineering will soon rival and potentially eclipse synthetic organic chemistry...
  55. pmc From the Cover: Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering
    Juan Huang
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Proc Natl Acad Sci U S A 106:8284-9. 2009
    ..We have successfully applied the genomic engineering scheme on 6 different genes and have generated at their loci more than 70 unique alleles...
  56. ncbi Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome
    Heinz Neumann
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nature 464:441-4. 2010
    ..As ribo-Q1 independently decodes a series of quadruplet codons, this work provides foundational technologies for the encoded synthesis and synthetic evolution of unnatural polymers in cells...
  57. ncbi An optical neural interface: in vivo control of rodent motor cortex with integrated fiberoptic and optogenetic technology
    Alexander M Aravanis
    Department of Bioengineering, Stanford University Medical Center, Stanford, CA 94305, USA
    J Neural Eng 4:S143-56. 2007
    ....
  58. pmc Modular control of multiple pathways using engineered orthogonal T7 polymerases
    Karsten Temme
    UCB UCSF Joint Graduate Group in Bioengineering, MC2540, Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, 1700 4th Street, San Francisco, CA 94158, USA
    Nucleic Acids Res 40:8773-81. 2012
    ....
  59. ncbi An efficient xylose-fermenting recombinant Saccharomyces cerevisiae strain obtained through adaptive evolution and its global transcription profile
    Yu Shen
    The State Key Laboratory of Microbial Technology, Shandong University, Shan Da Nan Road 27, Jinan 250100, China
    Appl Microbiol Biotechnol 96:1079-91. 2012
    ..Furthermore, the deletion of PHO13 decreased the xylose growth in the respiration deficiency strain although deleting PHO13 can improve the xylose metabolism in other strains...
  60. pmc Synthetic chromosome arms function in yeast and generate phenotypic diversity by design
    Jessica S Dymond
    High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    Nature 477:471-6. 2011
    ..When complete, the fully synthetic genome will allow massive restructuring of the yeast genome, and may open the door to a new type of combinatorial genetics based entirely on variations in gene content and copy number...
  61. pmc Synthetic gene networks that count
    Ari E Friedland
    Howard Hughes Medical Institute, Department of Biomedical Engineering, Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Science 324:1199-202. 2009
    ..These modular devices permit counting of varied user-defined inputs over a range of frequencies and can be expanded to count higher numbers...
  62. ncbi Customizing cell signaling using engineered genetic logic circuits
    Baojun Wang
    Department of Mathematics, Imperial College London, London, UK
    Trends Microbiol 20:376-84. 2012
    ....
  63. pmc Engineered bacteriophage targeting gene networks as adjuvants for antibiotic therapy
    Timothy K Lu
    Harvard Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:4629-34. 2009
    ..This work establishes a synthetic biology platform for the rapid translation and integration of identified targets into effective antibiotic adjuvants...
  64. pmc Late-acting dominant lethal genetic systems and mosquito control
    Hoang Kim Phuc
    Department of Zoology, University of Oxford, South Parks Road, Oxford, UK
    BMC Biol 5:11. 2007
    ..Additionally, vector populations with strong density-dependent effects will tend to be resistant to SIT-based control as the population-reducing effect of induced sterility will tend to be offset by reduced density-dependent mortality...
  65. pmc A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR
    Hiroki Torikai
    Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Blood 119:5697-705. 2012
    ..CAR(+)TCR(neg) T cells of this type may potentially have efficacy as an off-the-shelf therapy for investigational treatment of B-lineage malignancies...
  66. ncbi From zero to hero--design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production
    Judith Becker
    Institute of Biochemical Engineering, Technische Universitat Braunschweig, Braunschweig, Germany
    Metab Eng 13:159-68. 2011
    ..The design-based strategy for metabolic engineering reported here could serve as general concept for the rational development of microorganisms as efficient cellular factories for bio-production...
  67. ncbi A highly active synthetic mammalian retrotransposon
    Jeffrey S Han
    Department of Molecular Biology and Genetics and High Throughput Biology Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nature 429:314-8. 2004
    ..These synthetic retrotransposons are also the most highly active L1 elements known so far and have potential as practical tools for manipulating mammalian genomes...
  68. ncbi Engineering microbial consortia: a new frontier in synthetic biology
    Katie Brenner
    Division of Chemistry and Chemical Engineering, California Institute of Technology 210 41, Pasadena, CA 91125, USA
    Trends Biotechnol 26:483-9. 2008
    ..Here, we review recent efforts to engineer synthetic microbial consortia, and we suggest future applications...
  69. pmc High efficiency recombineering in lactic acid bacteria
    Jan Peter van Pijkeren
    Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA
    Nucleic Acids Res 40:e76. 2012
    ..We expect that this work will serve as a blueprint for the adaptation of ssDNA recombineering to other gram-positive bacteria...
  70. ncbi Narrowing down the targets: towards successful genetic engineering of drought-tolerant crops
    Shujun Yang
    Performance Plants Inc, 700 Gardiners Road, Kingston, Ontario, K7M 3X9, Canada
    Mol Plant 3:469-90. 2010
    ..The knowledge acquired will facilitate the genetic engineering of single or multiple targets and quantitative trait loci in key crops to create commercial-grade cultivars ..
  71. pmc Tracking genome engineering outcome at individual DNA breakpoints
    Michael T Certo
    Program in Molecular and Cellular Biology, University of Washington, Seattle, Washington, USA
    Nat Methods 8:671-6. 2011
    ....
  72. ncbi Model-driven engineering of RNA devices to quantitatively program gene expression
    James M Carothers
    California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley CA 94720, USA
    Science 334:1716-9. 2011
    ..More broadly, we provide a framework for studying RNA functions and illustrate the potential for the use of biochemical and biophysical modeling to develop biological design methods...
  73. pmc Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion
    Harris H Wang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Department of Genetics, Harvard Medical School, Harvard University, Boston, MA 02115, USA
    Nucleic Acids Res 39:7336-47. 2011
    ..These results further elucidate the mechanism of oligo-mediated allelic replacement (OMAR) and enable improved methodologies for efficient, large-scale engineering of genomes...
  74. ncbi Recombinase-mediated cassette exchange (RMCE): traditional concepts and current challenges
    Soeren Turan
    Hannover Medical School MHH, Experimental Hematology, Hannover, Germany
    J Mol Biol 407:193-221. 2011
    ..Remarkable efforts are noted in the framework of various mouse mutagenesis programs, which, in their first phase, have targeted virtually all genes and now start to shift their emphasis from gene trapping to gene modification...
  75. ncbi Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels
    Sung Kuk Lee
    Joint BioEnergy Institute, Emeryville, CA 95608, USA
    Curr Opin Biotechnol 19:556-63. 2008
    ....
  76. pmc Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization
    A J Link
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 179:6228-37. 1997
    ..coli. Because these strains are isogenic with the exception of their deleted ORFs, they may be used in competition with one another to reveal phenotypes not apparent when cultured singly...
  77. pmc Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases
    Erica A Moehle
    Sangamo BioSciences, Inc, Point Richmond Technology Center, 501 Canal Boulevard, Suite A100, Richmond, CA 94804, USA
    Proc Natl Acad Sci U S A 104:3055-60. 2007
    ....
  78. pmc Protein sequestration generates a flexible ultrasensitive response in a genetic network
    Nicolas E Buchler
    The Rockefeller University, New York, NY 10065, USA
    Mol Syst Biol 5:272. 2009
    ..The generation of flexible ultrasensitive responses is an unappreciated adaptive advantage that could explain the frequent evolutionary emergence of dominant negatives...
  79. pmc Engineering biological systems with synthetic RNA molecules
    Joe C Liang
    Division of Chemistry and Chemical Engineering, 1200 E California Boulevard, MC 210 41, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Cell 43:915-26. 2011
    ..Recent advances in implementing these engineered RNA molecules as key control elements in synthetic genetic networks are highlighting the functional relevance of this class of synthetic elements in programming cellular behaviors...
  80. ncbi Engineering yeasts for xylose metabolism
    Thomas W Jeffries
    USDA, Forest Service and University of Wisconsin Madison, Forest Products Laboratory, 53726, USA
    Curr Opin Biotechnol 17:320-6. 2006
    ..Lastly, strain selection through mutagenesis, adaptive evolution or from nature can also be employed to further improve activity...
  81. pmc Engineered bidirectional communication mediates a consensus in a microbial biofilm consortium
    Katie Brenner
    Division of Chemistry and Chemical Engineering, California Institute of Technology, MC 210 41, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 104:17300-4. 2007
    ..The microbial consensus consortium operates in diverse growth modes, including in a biofilm, where it sustains its response for several days...
  82. ncbi A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles
    Giuseppe Testa
    BIOTEC, Technische Universitat Dresden, c o Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
    Genesis 38:151-8. 2004
    ..Incorporation of site-specific recombination target sites creates a multipurpose allele for both knockout and conditional applications...
  83. ncbi Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides
    Joseph R Warner
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA
    Nat Biotechnol 28:856-62. 2010
    ..This approach can be applied to a broad range of traits to identify targets for future genome-engineering endeavors...
  84. pmc Engineering Corynebacterium glutamicum for isobutanol production
    Kevin Michael Smith
    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, 420 Westwood Plaza, Los Angeles, CA 90095, USA
    Appl Microbiol Biotechnol 87:1045-55. 2010
    ..9 g/L isobutanol in a pycldh background. These results show promise in engineering C. glutamicum for higher chain alcohol production using the 2-keto acid pathways...
  85. ncbi Chloroplast-derived vaccines against human diseases: achievements, challenges and scopes
    Andreas G Lössl
    Department of Applied Plant Sciences and Plant Biotechnology DAPP, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
    Plant Biotechnol J 9:527-39. 2011
    ..Particularly, plastid genetic engineering can be potentially used as a tool for cost-effective vaccine production...
  86. pmc Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
    A Joe Shaw
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 105:13769-74. 2008
    ....
  87. pmc Engineering of a plasmid-free Escherichia coli strain for improved in vivo biosynthesis of astaxanthin
    Karin Lemuth
    Institute of Microbiology, Universitat Stuttgart, Stuttgart, Germany
    Microb Cell Fact 10:29. 2011
    ..For the heterologous biosynthesis of astaxanthin in E. coli, however, the conversion of β-carotene to astaxanthin is obviously the most critical step towards an efficient biosynthesis of astaxanthin...
  88. ncbi Overproduction of free fatty acids in E. coli: implications for biodiesel production
    Xuefeng Lu
    Department of Chemistry, Stanford University, Stanford CA 94305, USA
    Metab Eng 10:333-9. 2008
    ..coli strain, with a specific productivity of 0.024 g/h/g dry cell mass and a peak conversion efficiency of 4.8% of the carbon source into fatty acid products. At least 50% of the fatty acids produced were present in the free acid form...
  89. pmc Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate
    J A Mosberg
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 186:791-9. 2010
    ..The mechanistic insights revealed by this work may facilitate further improvements to the versatility of lambda Red recombination...
  90. ncbi Terpenoids: opportunities for biosynthesis of natural product drugs using engineered microorganisms
    Parayil Kumaran Ajikumar
    Department of Chemical Engineering, Room 56 469, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Mol Pharm 5:167-90. 2008
    ..This review focuses on the biodiversity of terpenoids, the biosynthetic pathways involved, and engineering efforts to maximize the production through these pathways...
  91. ncbi Genomic deletions created upon LINE-1 retrotransposition
    Nicolas Gilbert
    Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Cell 110:315-25. 2002
    ..Thus, our data demonstrate multiple pathways for L1 integration in cultured cells, and show that L1 is not simply an insertional mutagen, but that its retrotransposition can result in significant deletions of genomic sequence...
  92. pmc Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii
    Beth A Rasala
    Department of Cell Biology and the Skaggs Institute for Chemical Biology, La Jolla, CA, USA
    Plant Biotechnol J 8:719-33. 2010
    ..Thus, the success rate, expression levels, and bioactivity achieved demonstrate the utility of Chlamydomonas reinhardtii as a robust platform for human therapeutic protein production...
  93. ncbi Combinatorial engineering of microbes for optimizing cellular phenotype
    Christine Nicole S Santos
    Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56 469, Cambridge, MA 02139, United States
    Curr Opin Chem Biol 12:168-76. 2008
    ....
  94. ncbi Meganucleases and DNA double-strand break-induced recombination: perspectives for gene therapy
    Frédéric Pâques
    Cellectis SA, 102, route de Noisy, 93 340 Romainville Cedex France
    Curr Gene Ther 7:49-66. 2007
    ..Finally, we will discuss the main issues that will need to be addressed in order to bring this promising technology to the patient...
  95. ncbi History of oncolytic viruses: genesis to genetic engineering
    Elizabeth Kelly
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Mol Ther 15:651-9. 2007
    ..Here, examination of early oncolytic virotherapy before genetic engineering serves to highlight tremendous advances, yet also hints at ways to penetrate host immune defenses, a ..
  96. ncbi Antioxidants in photosynthesis and human nutrition
    Barbara Demmig-Adams
    Department of Environmental, Population, and Organismic Biology, University of Colorado, Boulder, CO 80309 0334, USA
    Science 298:2149-53. 2002
    ..The pathways that synthesize these compounds are becoming amenable to genetic manipulation, which may yield benefits as widespread as improved plant stress tolerance and improved human physical and mental health...
  97. ncbi Molecular basis of xeroderma pigmentosum group C DNA recognition by engineered meganucleases
    Pilar Redondo
    Macromolecular Crystallography Group, Spanish National Cancer Research Centre CNIO, c Melchor Fdez Almagro 3, 28029 Madrid, Spain
    Nature 456:107-11. 2008
    ....
  98. ncbi Fermentative butanol production by Clostridia
    Sang Yup Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Biotechnol Bioeng 101:209-28. 2008
    ..The strategies for strain improvement by metabolic engineering and further requirements to make fermentative butanol production a successful industrial process are also discussed...
  99. pmc Genetic control of mammalian T-cell proliferation with synthetic RNA regulatory systems
    Yvonne Y Chen
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 107:8531-6. 2010
    ..By providing tight gene-expression control with customizable ligand inputs, RNA-based regulatory systems can greatly improve cellular therapies and advance broad applications in health and medicine...
  100. pmc Synthetic viruses: a new opportunity to understand and prevent viral disease
    Eckard Wimmer
    Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA
    Nat Biotechnol 27:1163-72. 2009
    ..Although only a few applications of virus synthesis have been described as yet, key recent findings have been the resurrection of the 1918 influenza virus and the generation of codon- and codon pair-deoptimized polioviruses...
  101. pmc Engineering of promoter replacement cassettes for fine-tuning of gene expression in Saccharomyces cerevisiae
    Elke Nevoigt
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Appl Environ Microbiol 72:5266-73. 2006
    ..cerevisiae was replaced by five TEF1 promoter mutants of different strengths, which allowed analysis of the impact of glycerol 3-phosphate dehydrogenase activity on the glycerol yield...

Research Grants62

  1. Role of CD44 in experimental Multiple Sclerosis
    Mitzi Nagarkatti; Fiscal Year: 2013
    ..abstract_text> ..
  2. O-GlcNAc Modification in Metabolic Homeostasis
    Xiaoyong Yang; Fiscal Year: 2013
    ..Pharmacological intervention to reset O-GlcNAc levels may represent an innovative strategy for preventing and treating metabolic syndrome. ..
  3. Genetics and Molecular Biology of Striated Muscle Myosin
    Sanford I Bernstein; Fiscal Year: 2013
    ..Overall our project will take advantage of an innovative experimental system to test significant questions regarding basic myosin function and disease. ..
  4. Oxidative Stress and Vascular HO in Diabetes
    Nader G Abraham; Fiscal Year: 2010
    ..It will allow for the first time, an in-depth analysis of the function of HO-1 in vascular protection and for the development of innovative gene-targeting therapies for the treatment of type I diabetes. ..
  5. Molecular Imaging of Protease Activation in Aneurysm
    Mehran M Sadeghi; Fiscal Year: 2013
    ..Furthermore, this approach may be applied to risk assessment and management of other vascular diseases, which together with aneurysm,are leading causes of mortality amongst veterans. ..
  6. Somatic cell gene transfer/neurological &clinical applications
    En Ju Lin; Fiscal Year: 2013
    ..We will genetically modify these cells to see if we can reduce seizures in an animal model of epilepsy with a second part of the project developing a safer way to control our genetic intervention. ..
  7. Identification of Transcriptional Biomarkers of Calorie Restriction in Adipose Ti
    JAMIE LOUIS BARGER; Fiscal Year: 2010
    ....
  8. BEHAVIORAL CORE
    Lennart Mucke; Fiscal Year: 2013
    ..abstract_text> ..
  9. THE PATHOBIOLOGY OF INHERITED RETINAL DEGENERATION
    Dean Bok; Fiscal Year: 2006
    ..abstract_text> ..
  10. NORMAL/PATHOLOGIC GENE EXPRESSION IN HEMATOPOIETIC CELLS
    GORDON GINDER; Fiscal Year: 1993
    ..The long term goal of this proposed Center of Excellence is to elucidate basic molecular mechanisms of disabling hematological diseases and apply this knowledge to innovative somatic genetic therapy approaches to treat these diseases...
  11. Hyperglycemia, Aldose Reducatse & Murine Atherosclerosis
    Ira Goldberg; Fiscal Year: 2005
    ..This is significant because it will provide for a model to study this complication and suggest a therapeutic target for prevention of diabetic macrovascular disease. ..
  12. Blocking of HIV Chemokine Receptors By Intrabodies
    Bruce Torbett; Fiscal Year: 2004
    ..Lastly, our proposed studies will contribute basic and pre-clinical insights into the feasibility of using HIV vectors to deliver and maintain ectopic gene expression in hematopoietic cells. ..
  13. Shifting Conceptions of Human Identity
    MICHAEL BESS; Fiscal Year: 2005
    ..abstract_text> ..
  14. NEURAL BASIS OF INTERNAL REPRESENTATION OF PLACE
    Bruce McNaughton; Fiscal Year: 2009
    ..A well characterized and well understood rodent model of hippocampal function will also provide a platform for drug discovery and the development of genetic intervention for treatment of human memory disorder. ..
  15. Non-invasive cardio-pulmonary monitor for mice
    Martin Baruch; Fiscal Year: 2007
    ..is growing along with an increasing population of mice strains that are being used to test drug responses, genetic engineering attempts, and other factors that can affect these biological rhythms...
  16. Generation of kinase-resistant NMDA receptors by gene-targeting in the mouse
    John Leonard; Fiscal Year: 2009
    ..These mice will be made available as a research tool for neuroscientists worldwide. ..
  17. STRUCTURE OF RIBONUCLEIC ACIDS
    Stephen Harvey; Fiscal Year: 2005
    ..This research will include development of a library of RNA structural motifs derived from the Nucleic Acid Database, along with tools for incorporating these motifs into structural models. ..
  18. New Genetic Intervention for Healthy Aging
    James Nelson; Fiscal Year: 2005
    ..A PTP-1B-/- longevity mutant would also provide a new target for pharmacologic interventions to retard aging. ..
  19. GENE THERAPY FOR OVARIAN CANCER
    David Curiel; Fiscal Year: 1999
    ..Finally, in vivo studies will be carried out to develop the methods for translation of these strategies into a gene therapy approach for ovarian carcinoma. ..
  20. Limbic Dopaminergic System in Major Depression
    Gregory Ordway; Fiscal Year: 2005
    ..Uncovering the potential role of DA in the pathobiology of depression may lead to advancements in the pharmacological, and possibly genetic, intervention of major depression. ..
  21. EVALUATING USE OF GENETIC INFORMATION: A MODEL PROCESS
    Wylie Burke; Fiscal Year: 2003
    ..5. To conduct an evaluation of the model process within a large health care system. ..
  22. MUCOSAL GENE TRANSFER USING DENDRIMER POLYMERS
    James Baker; Fiscal Year: 1999
    ..The results of these studies will provide basic information about gene delivery that should allow further refinements in protocols and agents for endobronchial gene transfer. ..
  23. SUBSTRATE METABOLISM IN ISOLATED MYOCYTES IN SHOCK
    Maw Shung Liu; Fiscal Year: 2002
    ..The results obtained will lay the groundwork for future therapy by drugs or genetic intervention to preserve cardiac function during sepsis. ..
  24. Genetic Determination of Host Processes for HIV-1
    Shigemi Kinoshita; Fiscal Year: 2004
    ..Findings from this project will allow us to characterize the biology of HIV-1 in human cells in greater detail and will provide new approaches to exploit molecular genetic intervention to understand and treat AIDS. ..
  25. Tolerance to Carbohydrate Antigens in Transplantation
    David Cooper; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  26. Biosensors for metabolic engineering
    Jay Keasling; Fiscal Year: 2006
    ..abstract_text> ..
  27. Chimeric Nucleases for Gene Targeting in Drosophila
    Dana Carroll; Fiscal Year: 2003
    ..To test the generality of the method, it will be applied to a second Drosophila locus, the rosy (ry) gene. ..
  28. Thromboregulation to Prevent Thrombotic Microangiopathy
    David Cooper; Fiscal Year: 2008
    ..It is anticipated that these approaches will abrogate the development of TM and allow long-term survival of pig organs in baboons. ..
  29. Stimulating Gene Targeting with Zinc Finger Nucleases
    Dana Carroll; Fiscal Year: 2004
    ..To demonstrate the generality of this approach to gene targeting, it will be applied to another mouse locus, the Rpo2-1 gene. ..
  30. GENETIC AND BIOCHEMICAL STUDIES OF PHOB ACTIVATION
    WILLIAM MCCLEARY; Fiscal Year: 2003
    ..An increased understanding of these signal transduction proteins will assist in the rational design of drugs to combat pathogens. ..
  31. SALIVARY PROTEINS IN DENTAL INTEGUMENTS
    FRANK OPPENHEIM; Fiscal Year: 2009
    ..abstract_text> ..
  32. Studies of naturally occurring structurally modified carbohydrates
    Xi Chen; Fiscal Year: 2010
    ..abstract_text> ..
  33. A POLYMERIC DRUG DELIVERY SYSTEM FOR CANCER THERAPY
    JINDRICH H KOPECEK; Fiscal Year: 2010
    ..The concept of double-targeted macromolecular therapeutics provides a new paradigm for the design of efficient anticancer drugs for the treatment of ovarian cancer. ..
  34. Engineering AAV Vectors to Evade Antibody Neutralization
    David V Schaffer; Fiscal Year: 2010
    ..Furthermore, it will yield insights into the responses of other immune system components to AAV. ..
  35. Neural Progenitor Cells as Cancer Therapy Vectors
    Karen Aboody; Fiscal Year: 2009
    ..The described approach might then be adapted to the treatment of other solid tumors by careful choice of appropriate therapeutic transgenes. ..
  36. DNA Patent Policies at Academic Institutions
    LeRoy Walters; Fiscal Year: 2003
    ..S. and Canadian academic institutions. ..
  37. Mucosal Immunization with a Vaccinia Virus-Based Vector
    Bertram Jacobs; Fiscal Year: 2003
    ..Animals will be immunized intra-nasally either with virus alone or as part of a prime-boost regime. Systemic, nasal and vaginal immune responses to the HIV multi-epitope polypeptide will be analyzed. ..
  38. BD Biosciences FACS Canto Flow Cytometer
    LAWRENCE LAMB; Fiscal Year: 2006
    ..The laboratory will require an instrument that will serve the laboratory in the development and execution of advanced cell therapy trials. [unreadable] [unreadable]..
  39. The Role of Metabolic Signaling in Longevity Regulation
    Su Ju Lin; Fiscal Year: 2010
    ..Many cellular pathways are conserved in yeast and mammals. Therefore, insight into these molecular processes in yeast will also provide clues to the molecular basis of human aging and age-associated diseases. ..
  40. EUROPIC america 2002: a conference for picornaviruses
    Eckard Wimmer; Fiscal Year: 2002
    ..The over will discuss the difficulties (circulation of vaccine strains, recent outbreak of poliomyelitis in the Western Hemisphere) that have emerged during the current, historic effect to globally eradicate poliovirus. ..
  41. REGULATION OF B LYMPHOMA GROWTH AND APOPTOSIS
    David Scott; Fiscal Year: 2001
    ..The results of these studies will provide information on regulation of neoplastic growth as well as mechanism for inducing apoptosis in a model for deletional tolerance. ..
  42. TOOLS FOR PUBLIC CONVERSATION ABOUT BEHAVIORAL GENETICS
    Erik Parens; Fiscal Year: 2001
    ....
  43. SHIGA LIKE TOXIN AND RENAL CELL DYSFUNCTION
    Donald Kohan; Fiscal Year: 2001
    ..These studies provide essential information on how and why HGEN are damaged in HUS. ..
  44. Structure/function analysis of the E. coli NIR/NRII signal transduction system
    Alexander Ninfa; Fiscal Year: 2009
    ..Our studies with a model system should allow rapid progress. ..
  45. Cellular Injury and Adaptation
    J Cobb; Fiscal Year: 2008
    ..A well-characterized therapy that improves Th1 responsiveness and survival after CLP, systemic IL-12, will be used to perturb the system, testing the robustness of the models. ..
  46. XENOBIOTICS AND ALLERGIC INFLAMMATION
    Andrew Saxon; Fiscal Year: 2005
    ..The projects are supported by a DEP collection and fractionation core, a mouse core, and a administrative, patient and sample coordination core. ..
  47. Approach for amplification of molecular signals
    ALEXEY ZDANOVSKY; Fiscal Year: 2005
    ..Bacterial luciferase will be incorporated in the naturally existing cascades of enzymatic reactions to produce an inexpensive and simple assay for the detection of antigens of interest including those of biowarfare agents. ..
  48. BLUETONGUE VIRUS MORPHOGENESIS
    Polly Roy; Fiscal Year: 2005
    ..Together with the proposed experimental programme, our reagent and knowledge base will allow us to complete the tasks we have specified to offer new insights into virus replication and new suggestions for effective control. ..
  49. Suspension arrays detection/quantification of antibodies
    ALEXEY ZDANOVSKY; Fiscal Year: 2005
    ....
  50. Structure and Function of IRES Elements
    Eckard Wimmer; Fiscal Year: 2006
    ..We will investigate the molecular reason for this attenuation. Finally, we will determine whether we can exchange viral IRESs also IRESs of cellular mRNA. ..
  51. MICROBIAL DEGRADATION OF AGENT ORANGE
    Ananda Chakrabarty; Fiscal Year: 2004
    ..The progress achieved and the ways we intend to meet our goals are detailed in this proposal ..
  52. Role of the E3L gene in poxvirus pathogenesis
    Bertram Jacobs; Fiscal Year: 2007
    ..Thus, this work will lead to translation of basic molecular knowledge of E3L function into clinically relevant applications. ..
  53. Characterization of microRNA cell death regulators
    Bruce Hay; Fiscal Year: 2007
    ..Therefore, it is likely that successful completion of the proposed experiments will increase our understanding of how cell death is regulated in human health and disease. [unreadable] [unreadable] [unreadable]..
  54. APP-BP1, The Cell Cycle, and Alzheimer Disease
    Rachael Neve; Fiscal Year: 2007
    ..We will test whether these predictions are correct. [unreadable] [unreadable]..
  55. Novel Strategy for Animal Model Testing of HCMV Vaccines
    Mark Schleiss; Fiscal Year: 2007
    ..These studies will represent the first efficacy test of a human CMV subunit vaccine in a small animal model of congenital CMV infection. [unreadable] [unreadable]..
  56. Engineering Novel AAV Vectors for Retinal Gene Therapy
    David Schaffer; Fiscal Year: 2007
    ..The novel approaches developed in this work will have general impact for the molecular engineering of enhanced viral gene delivery vehicles for a number of retinal diseases. [unreadable] [unreadable] [unreadable]..
  57. Nonapoptotic roles for proteases in spermatogenesis
    Bruce Hay; Fiscal Year: 2008
    ..In addition this work is likely to provide insight into the mechanisms by which caspase activity, and the consequences of caspase activation, are regulated. ..
  58. Pathogenesis of EAST1 toxin in ETEC associated diarrhea disease
    Weiping Zhang; Fiscal Year: 2008
    ..The determination of virulence significance of EAST1 toxin in diarrheal disease will provide essential information to prevent or control this disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  59. Blood Systems Biology
    Daniel Hammer; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  60. Target Specificity of the Yeast Retrotransposon Ty5
    Daniel Voytas; Fiscal Year: 2005
    ....
  61. GENETIC AND CHEMICAL APPROACHES TO NOVEL LIPID II BINDING PEPTIDE ANTIBIOTICS
    Xihou Yin; Fiscal Year: 2010
    ..Understanding the molecular basis of enduracidin resistance in the producing organism will help predict how new antibiotics may be affected by existing resistance mechanisms and provide insight into ways that resistance may emerge. ..