Summary: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

Top Publications

  1. ncbi Complete replication of hepatitis C virus in cell culture
    Brett D Lindenbach
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:623-6. 2005
  2. pmc Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death
    M C Wei
    Howard Hughes Medical Institute, Departments of Pathology and Medicine, Harvard Medical School, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 292:727-30. 2001
  3. pmc A simplified system for generating recombinant adenoviruses
    T C He
    The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 95:2509-14. 1998
  4. ncbi Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha
    D R Alessi
    Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, Scotland
    Curr Biol 7:261-9. 1997
  5. ncbi Mdm2 promotes the rapid degradation of p53
    Y Haupt
    Lautenberg Center for General and Tumor Immunology, The Hebrew University Haddassah Medical School, Jerusalem, Israel
    Nature 387:296-9. 1997
  6. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002
  7. ncbi Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
    Z Ivics
    Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108 1095, USA
    Cell 91:501-10. 1997
  8. ncbi MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells
    Jennifer A Chan
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 65:6029-33. 2005
  9. pmc High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells
    Yves Durocher
    Animal Cell Technology and Downstream Processing Group, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada
    Nucleic Acids Res 30:E9. 2002
  10. ncbi Stat3 as an oncogene
    J F Bromberg
    Laboratory of Molecular Cell Biology, The Rockefeller University, New York, New York 10021 6399, USA
    Cell 98:295-303. 1999

Detail Information

Publications408 found, 100 shown here

  1. ncbi Complete replication of hepatitis C virus in cell culture
    Brett D Lindenbach
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:623-6. 2005
    ..HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals...
  2. pmc Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death
    M C Wei
    Howard Hughes Medical Institute, Departments of Pathology and Medicine, Harvard Medical School, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 292:727-30. 2001
    ..Thus, activation of a "multidomain" proapoptotic member, BAX or BAK, appears to be an essential gateway to mitochondrial dysfunction required for cell death in response to diverse stimuli...
  3. pmc A simplified system for generating recombinant adenoviruses
    T C He
    The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 95:2509-14. 1998
    ..Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes...
  4. ncbi Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha
    D R Alessi
    Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, Scotland
    Curr Biol 7:261-9. 1997
    ..The phosphorylation of both residues is required for maximal activation of PKB. The kinases that phosphorylate PKB are, however, unknown...
  5. ncbi Mdm2 promotes the rapid degradation of p53
    Y Haupt
    Lautenberg Center for General and Tumor Immunology, The Hebrew University Haddassah Medical School, Jerusalem, Israel
    Nature 387:296-9. 1997
    ..During recovery from DNA damage, maximal Mdm2 induction coincides with rapid p53 loss. We propose that the Mdm2-promoted degradation of p53 provides a new mechanism to ensure effective termination of the p53 signal...
  6. ncbi Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells
    David A Zacharias
    Department of Pharmacology, Biomedical Sciences Graduate Program, and, Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093 0647, USA
    Science 296:913-6. 2002
    ..Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane...
  7. ncbi Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
    Z Ivics
    Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108 1095, USA
    Cell 91:501-10. 1997
    ..Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis...
  8. ncbi MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells
    Jennifer A Chan
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 65:6029-33. 2005
    ..Our data suggest that aberrantly expressed miR-21 may contribute to the malignant phenotype by blocking expression of critical apoptosis-related genes...
  9. pmc High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells
    Yves Durocher
    Animal Cell Technology and Downstream Processing Group, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada
    Nucleic Acids Res 30:E9. 2002
    A scalable transfection procedure using polyethylenimine (PEI) is described for the human embryonic kidney 293 cell line grown in suspension...
  10. ncbi Stat3 as an oncogene
    J F Bromberg
    Laboratory of Molecular Cell Biology, The Rockefeller University, New York, New York 10021 6399, USA
    Cell 98:295-303. 1999
    ..Thus, the activated Stat3 molecule by itself can mediate cellular transformation and the experiments focus attention on the importance of constitutive Stat3 activation in human tumors...
  11. pmc The impact of microRNAs on protein output
    Daehyun Baek
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 455:64-71. 2008
    ..The impact of microRNAs on the proteome indicated that for most interactions microRNAs act as rheostats to make fine-scale adjustments to protein output...
  12. pmc The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2
    Sun Mi Park
    The Ben May Department for Cancer Research, The University of Chicago, Chicago, Illinois 60637, USA
    Genes Dev 22:894-907. 2008
    ..Conversely, inhibition of miR-200 reduced E-cadherin expression, increased expression of Vimentin, and induced EMT. Our data identify miR-200 as a powerful marker and determining factor of the epithelial phenotype of cancer cells...
  13. doi Widespread changes in protein synthesis induced by microRNAs
    Matthias Selbach
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13125 Berlin, Germany
    Nature 455:58-63. 2008
    ..used a new proteomic approach to measure changes in synthesis of several thousand proteins in response to miRNA transfection or endogenous miRNA knockdown. In parallel, we quantified mRNA levels using microarrays...
  14. doi microRNA-7 inhibits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma
    Benjamin Kefas
    Division of Neuro Oncology, Neurology Department, University of Virginia Health System, Charlottesville, Virginia, USA
    Cancer Res 68:3566-72. 2008
    ..Importantly, transfection with miR-7 decreased viability and invasiveness of primary glioblastoma lines...
  15. ncbi Regulation of p53 stability by Mdm2
    M H Kubbutat
    ABL Basic Research Program, NCI FCRDC, Frederick, Maryland 21702 1201, USA
    Nature 387:299-303. 1997
    ..Furthermore, mechanisms regulating the Mdm2-induced degradation of p53 may play a role in controlling the extent and duration of the p53 response...
  16. ncbi Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
    H Niwa
    Centre for Genome Research, The University of Edinburgh, King s Buildings, Edinburgh, UK
    Nat Genet 24:372-6. 2000
  17. pmc Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line
    Philip J Reeves
    Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:13419-24. 2002
  18. ncbi A system for stable expression of short interfering RNAs in mammalian cells
    Thijn R Brummelkamp
    Division of Molecular Carcinogenesis, Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, Netherlands
    Science 296:550-3. 2002
    ..Therefore, the pSUPER vector constitutes a new and powerful system to analyze gene function in a variety of mammalian cell types...
  19. pmc MiRNA-directed regulation of VEGF and other angiogenic factors under hypoxia
    Zhong Hua
    Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China
    PLoS ONE 1:e116. 2006
    ..The competitive principle, differential regulation, multi-miRNA binding sites, and false positive miRNAs might be useful strategies in the avoidance of unwanted cross-action among genes targeted by miRNAs with multiple targets...
  20. pmc Selective blockade of microRNA processing by Lin28
    Srinivas R Viswanathan
    Stem Cell Program, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
    Science 320:97-100. 2008
    ..Our results identify Lin28 as a negative regulator of miRNA biogenesis and suggest that Lin28 may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers...
  21. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
    ..Our data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling...
  22. ncbi High efficiency transfection of Plasmodium berghei facilitates novel selection procedures
    Chris J Janse
    Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
    Mol Biochem Parasitol 145:60-70. 2006
    The use of transfection in the study of the biology of malaria parasites has been limited due to poor transfection efficiencies (frequency of 10(-6) to 10(-9)) and a paucity of selection markers...
  23. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
    ..Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria...
  24. ncbi RIG-I-mediated antiviral responses to single-stranded RNA bearing 5'-phosphates
    Andreas Pichlmair
    Immunobiology Laboratory, Cancer Research UK, London Research Institute, London WC2A 3PX, UK
    Science 314:997-1001. 2006
  25. ncbi Role for a bidentate ribonuclease in the initiation step of RNA interference
    E Bernstein
    Cold Spring Harbor Laboratory, New York 11724, USA
    Nature 409:363-6. 2001
    ..The enzyme has a distinctive structure, which includes a helicase domain and dual RNase III motifs. Dicer also contains a region of homology to the RDE1/QDE2/ARGONAUTE family that has been genetically linked to RNAi...
  26. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
    ..iPSCs fulfilled all criteria of pluripotency, such as pluripotency gene expression, teratoma formation and contribution to chimeras. piggyBac transposon-based reprogramming may be used to generate therapeutically applicable iPSCs...
  27. ncbi Activation of the ATM kinase by ionizing radiation and phosphorylation of p53
    C E Canman
    The Johns Hopkins School of Medicine, Oncology Center, Baltimore, MD 21205, USA
    Science 281:1677-9. 1998
    ..These observations, along with the fact that phosphorylation of p53 on serine-15 in response to ionizing radiation is reduced in ataxia telangiectasia cells, suggest that ATM is a protein kinase that phosphorylates p53 in vivo...
  28. ncbi 5'-Triphosphate RNA is the ligand for RIG-I
    Veit Hornung
    Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany
    Science 314:994-7. 2006
  29. pmc The cyclin D1 gene is a target of the beta-catenin/LEF-1 pathway
    M Shtutman
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 96:5522-7. 1999
    ..Increased beta-catenin levels may thus promote neoplastic conversion by triggering cyclin D1 gene expression and, consequently, uncontrolled progression into the cell cycle...
  30. pmc Production of infectious hepatitis C virus in tissue culture from a cloned viral genome
    Takaji Wakita
    Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo 183 8526, Japan
    Nat Med 11:791-6. 2005
    ..Here we show that the JFH1 genome replicates efficiently and supports secretion of viral particles after transfection into a human hepatoma cell line (Huh7). Particles have a density of about 1.15-1...
  31. pmc Electroporation and RNA interference in the rodent retina in vivo and in vitro
    Takahiko Matsuda
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:16-22. 2004
    ..Plasmid DNA was also efficiently transfected into retinal explants in vitro by high-voltage pulses...
  32. doi Comprehensive MicroRNA profiling for head and neck squamous cell carcinomas
    Angela B Y Hui
    Division of Applied Molecular Oncology, Ontario Cancer Institute, University of Toronto, Toronto, Ontario, Canada
    Clin Cancer Res 16:1129-39. 2010
    ..The objective of this study is to investigate the significance of microRNAs (miRNA) in patients with locally advanced head and neck squamous cell carcinoma (HNSCC)...
  33. ncbi let-7 microRNA functions as a potential growth suppressor in human colon cancer cells
    Yukihiro Akao
    Department of Genetic Diagnosis, Gifu International Institute of Biotechnology, Kakamigahara, Japan
    Biol Pharm Bull 29:903-6. 2006
    ..At that time, the levels of RAS and c-myc proteins were lowered after the transfection, whereas the levels of both of their mRNAs remained almost unchanged...
  34. ncbi Switching from repression to activation: microRNAs can up-regulate translation
    Shobha Vasudevan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 318:1931-4. 2007
    ..Thus, activation is a common function of microRNPs on cell cycle arrest. We propose that translation regulation by microRNPs oscillates between repression and activation during the cell cycle...
  35. ncbi Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery
    Ikramy A Khalil
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Pharmacol Rev 58:32-45. 2006
    ..In addition, available knowledge concerning cellular entry and the intracellular trafficking of cationic lipid-DNA complexes (lipoplexes) and cationic polymer-DNA complexes (polyplexes) is summarized...
  36. pmc Methylation mediated silencing of MicroRNA-1 gene and its role in hepatocellular carcinogenesis
    Jharna Datta
    Department of Molecular and Cellular Biochemistry, College of Pharmacy, and Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210, USA
    Cancer Res 68:5049-58. 2008
    ..The inhibition of cell cycle progression and induction of apoptosis after re-expression of miR-1 are some of the mechanisms by which DNA hypomethylating agents suppress hepatocarcinoma cell growth...
  37. ncbi Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6
    Z Zhong
    Laboratory of Molecular Cell Biology, Rockefeller University, New York, NY 10021
    Science 264:95-8. 1994
    ..Differential activation of different STAT proteins in response to different ligands should help to explain specificity in nuclear signaling from the cell surface...
  38. pmc MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer
    Fanyin Meng
    Department of Internal Medicine, Scott and White Clinic, Texas A and M University System Health Science Center College of Medicine, Temple, Texas, USA
    Gastroenterology 133:647-58. 2007
    ..Although a role for aberrant miRNA expression in cancer has been postulated, the pathophysiologic role and relevance of aberrantly expressed miRNA to tumor biology has not been established...
  39. ncbi A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
    ..With the improved speed and efficiency of maturation and the increased resistance to environment, Venus will enable fluorescent labelings that were not possible before...
  40. ncbi A telomerase component is defective in the human disease dyskeratosis congenita
    J R Mitchell
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Nature 402:551-5. 1999
    ..The pathology of DKC is consistent with compromised telomerase function leading to a defect in telomere maintenance, which may limit the proliferative capacity of human somatic cells in epithelia and blood...
  41. pmc MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells
    Margaret S Ebert
    Center for Cancer Research, E17 529B Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Methods 4:721-6. 2007
    ..RNA polymerase II promoter (Pol II)-driven sponges contain a fluorescence reporter gene for identification and sorting of sponge-treated cells. We envision the use of stably expressed sponges in animal models of disease and development...
  42. ncbi Signalling downstream of activated mammalian Notch
    S Jarriault
    Unité de Biologie Moléculaire de l Expression Génique, URA 1149 CNRS, Institut Pasteur, Paris, France
    Nature 377:355-8. 1995
    ..Here we show that activated forms of mNotch associate with the human analogue of Su(H), KBF2/RBP-J kappa (refs 8,9) and act as transcriptional activators through the KBF2-binding sites of the HES-1 promoter...
  43. ncbi Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery
    Jez G Carlton
    Department of Infectious Diseases, King s College London School of Medicine, London, UK
    Science 316:1908-12. 2007
    ..Thus, HIV-1 budding and cytokinesis use a similar subset of cellular components to carry out topologically similar membrane fission events...
  44. ncbi Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis
    R U Jänicke
    Institute of Molecular and Cell Biology, The National University of Singapore, 30 Medical Drive, Singapore 117609, Republic of Singapore
    J Biol Chem 273:9357-60. 1998
    ..These results indicate that although caspase-3 is not essential for TNF- or staurosporine-induced apoptosis, it is required for DNA fragmentation and some of the typical morphological changes of cells undergoing apoptosis...
  45. ncbi hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase
    H Vaziri
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 107:149-59. 2001
    ..In contrast, expression of a catalytically inactive hSir2 protein potentiates p53-dependent apoptosis and radiosensitivity. We propose that hSir2 is involved in the regulation of p53 function via deacetylation...
  46. ncbi Dynamic interactions of cortactin and membrane type 1 matrix metalloproteinase at invadopodia: defining the stages of invadopodia formation and function
    Vira V Artym
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical School, Washington, District of Columbia 20057 1469, USA
    Cancer Res 66:3034-43. 2006
    ..Based on these results, we propose a stepwise model of invadopodia formation and function...
  47. pmc Amyloidogenic processing of the Alzheimer beta-amyloid precursor protein depends on lipid rafts
    Robert Ehehalt
    Max Planck Institute of Molecular Cell Biology and Genetics, D 01307 Dresden, Germany
    J Cell Biol 160:113-23. 2003
    ..Thus, access of alpha- and beta-secretase to APP, and therefore A beta generation, may be determined by dynamic interactions of APP with lipid rafts...
  48. pmc Robust hepatitis C virus infection in vitro
    Jin Zhong
    Departments of Molecular and Experimental Medicine and Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 102:9294-9. 2005
    ..This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen...
  49. ncbi Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase
    M Tewari
    Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, USA
    Cell 81:801-9. 1995
    ..Furthermore, CrmA blocked cleavage of PARP in cells undergoing apoptosis. We propose that Yama may represent an effector component of the mammalian cell death pathway and suggest that CrmA blocks apoptosis by inhibiting Yama...
  50. ncbi Activating Smoothened mutations in sporadic basal-cell carcinoma
    J Xie
    Department of Dermatology, San Francisco General Hospital, University of California, 94110, USA
    Nature 391:90-2. 1998
    ..These findings support the role of SMO as a signalling component of the SHH-receptor complex and provide direct evidence that mutated SMO can function as an oncogene in BCCs...
  51. ncbi DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells
    T B Geijtenbeek
    Department of Tumor Immunology, University Medical Center St Radboud, Nijmegen, The Netherlands
    Cell 100:587-97. 2000
    ..We propose that DC-SIGN efficiently captures HIV-1 in the periphery and facilitates its transport to secondary lymphoid organs rich in T cells, to enhance infection in trans of these target cells...
  52. pmc Loss of A-type lamin expression compromises nuclear envelope integrity leading to muscular dystrophy
    T Sullivan
    Advanced BioScience Laboratories Basic Research Program, National Cancer Institute Frederick Cancer Research and Development Center, Maryland 21702, USA
    J Cell Biol 147:913-20. 1999
    ..Mice lacking the A-type lamins exhibit tissue-specific alterations to their nuclear envelope integrity and emerin distribution. In skeletal and cardiac muscles, this is manifest as a dystrophic condition related to EDMD...
  53. ncbi Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation
    P Kavsak
    Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto M5G 1X5, Canada
    Mol Cell 6:1365-75. 2000
    ..These studies thus define Smad7 as an adaptor in an E3 ubiquitin-ligase complex that targets the TGF beta receptor for degradation...
  54. ncbi Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin
    E Carballo
    Office of Clinical Research and Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Science 281:1001-5. 1998
    ..These findings identify TTP as a component of a negative feedback loop that interferes with TNF-alpha production by destabilizing its messenger RNA. This pathway represents a potential target for anti-TNF-alpha therapies...
  55. ncbi Hereditary early-onset Parkinson's disease caused by mutations in PINK1
    Enza Maria Valente
    CSS IRCCS, Mendel Institute, Viale Regina Margherita 261, 00198 Rome, Italy
    Science 304:1158-60. 2004
    ..These data provide a direct molecular link between mitochondria and the pathogenesis of PD...
  56. pmc Disrupting the pairing between let-7 and Hmga2 enhances oncogenic transformation
    Christine Mayr
    Howard Hughes Medical Institute and Department of Biology, Massachusetts Institute of Technology, and Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 315:1576-9. 2007
    ..Thus, losing miRNA-directed repression of an oncogene provides a mechanism for tumorigenesis, and disrupting a single miRNA-target interaction can produce an observable phenotype in mammalian cells...
  57. pmc In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation
    Akihisa Matsuyama
    Chemical Genetics Laboratory, Wako, Saitama 351 0198, Japan
    EMBO J 21:6820-31. 2002
    ..We therefore suggest that acetylation and deacetylation are coupled to the microtubule turnover and that HDAC6 plays a key regulatory role in the stability of the dynamic microtubules...
  58. pmc Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands
    K Morita
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    Proc Natl Acad Sci U S A 96:511-6. 1999
    ..These findings indicated that multiple claudin family members are involved in the formation of TJ strands in various tissues...
  59. pmc The tumour-suppressive function of miR-1 and miR-133a targeting TAGLN2 in bladder cancer
    H Yoshino
    Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8 35 1 Sakuragaoka, Kagoshima 890 8520, Japan
    Br J Cancer 104:808-18. 2011
    ..In this study, we focussed on the functional significance of miR-1 and miR-133a in BC cell lines and identified a molecular network of these miRNAs...
  60. ncbi A time-dependent phase shift in the mammalian unfolded protein response
    Hiderou Yoshida
    Graduate School of Biostudies, Kyoto University, Kyoto 606 8304, Japan
    Dev Cell 4:265-71. 2003
  61. ncbi Tumor suppressor p53 is a direct transcriptional activator of the human bax gene
    T Miyashita
    La Jolla Cancer Research Foundation, California 92037
    Cell 80:293-9. 1995
    ..Taken together, the results suggest that bax is a p53 primary-response gene, presumably involved in a p53-regulated pathway for induction of apoptosis...
  62. ncbi The cloned capsaicin receptor integrates multiple pain-producing stimuli
    M Tominaga
    Department of Cellular and Molecular Pharmacology, W M Keck Foundation Center for Integrative Neuroscience, University of California, San Francisco 94143, USA
    Neuron 21:531-43. 1998
    ..A role for VR1 in injury-induced hypersensitivity at the level of the sensory neuron is presented...
  63. doi Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers
    Igor I Slowing
    Department of Chemistry and US Department of Energy Ames Laboratory, Iowa State University, Ames, Iowa 50011 3111, USA
    Adv Drug Deliv Rev 60:1278-88. 2008
    ..We envision that these MSN-based systems have a great potential for a variety of drug delivery applications, such as the site-specific delivery and intracellular controlled release of drugs, genes, and other therapeutic agents...
  64. pmc Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin
    Hideki Yamaguchi
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 168:441-52. 2005
    ..These results indicate that EGF receptor signaling regulates invadopodium formation through the N-WASP-Arp2/3 pathway and cofilin is necessary for the stabilization and maturation of invadopodia...
  65. ncbi Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40
    Emilie Vander Haar
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 6 155 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA
    Nat Cell Biol 9:316-23. 2007
    ..These findings identify PRAS40 as an important regulator of insulin sensitivity of the Akt-mTOR pathway and a potential target for the treatment of cancers, insulin resistance and hamartoma syndromes...
  66. pmc miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer
    T Chiyomaru
    Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8 35 1 Sakuragaoka, Kagoshima, Japan
    Br J Cancer 102:883-91. 2010
    ..We have recently identified down-regulated microRNAs including miR-145 and miR-133a in bladder cancer (BC). The aim of this study is to determine the genes targeted by miR-145, which is the most down-regulated microRNA in BC...
  67. pmc Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs
    Aly A Khan
    Department of Computer Science, Columbia University, New York, New York, USA
    Nat Biotechnol 27:549-55. 2009
    b>Transfection of small RNAs (such as small interfering RNAs (siRNAs) and microRNAs (miRNAs)) into cells typically lowers expression of many genes. Unexpectedly, increased expression of genes also occurs...
  68. ncbi An essential role for NF-kappaB in preventing TNF-alpha-induced cell death
    A A Beg
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 274:782-4. 1996
    ..These results have implications for the treatment of inflammatory and proliferative diseases...
  69. ncbi Oscillations in NF-kappaB signaling control the dynamics of gene expression
    D E Nelson
    Centre for Cell Imaging, School of Biological Sciences, Bioscience Research Building, Crown Street, Liverpool, L69 7ZB, UK
    Science 306:704-8. 2004
    ..The functional consequences of NF-kappaB signaling may thus depend on number, period, and amplitude of oscillations...
  70. ncbi A class of membrane proteins shaping the tubular endoplasmic reticulum
    Gia K Voeltz
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 124:573-86. 2006
    ..The simultaneous absence of the reticulons and Yop1p in S. cerevisiae results in disrupted tubular ER. We propose that these "morphogenic" proteins partition into and stabilize highly curved ER membrane tubules...
  71. ncbi Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA
    S Bauer
    Fred Hutchinson Cancer Research Center, Clinical Research Division, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Science 285:727-9. 1999
    ..These results define an activating immunoreceptor-MHC ligand interaction that may promote antitumor NK and T cell responses...
  72. pmc A human dynamin-related protein controls the distribution of mitochondria
    E Smirnova
    Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California 90095 1737, USA
    J Cell Biol 143:351-8. 1998
    ..The function of this new dynamin-related protein in organelle morphology represents a novel role for a member of the dynamin family of proteins...
  73. ncbi Identification of Smad7, a TGFbeta-inducible antagonist of TGF-beta signalling
    A Nakao
    Ludwig Institute for Cancer Research, Uppsala, Sweden
    Nature 389:631-5. 1997
    ..Here we report the identification of Smad7, which is related to Smad6. Transfection of Smad7 blocks responses mediated by TGF-beta in mammalian cells, and injection of Smad7 RNA into Xenopus ..
  74. pmc HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription
    Nanhai He
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 38:428-38. 2010
    ..The ability of Tat to enable two different classes of elongation factors to cooperate and coordinate their actions on the same polymerase enzyme explains why Tat is such a powerful activator of HIV-1 transcription...
  75. ncbi BMP-7 counteracts TGF-beta1-induced epithelial-to-mesenchymal transition and reverses chronic renal injury
    Michael Zeisberg
    Center for Matrix Biology, Gastroenterology and Renal Divisions, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nat Med 9:964-8. 2003
    ..Collectively, these results provide evidence of cross talk between BMP-7 and TGF-beta1 in the regulation of EMT in health and disease...
  76. ncbi Transfection of human hepatocyte growth factor gene ameliorates secondary lymphedema via promotion of lymphangiogenesis
    Yukihiro Saito
    Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Circulation 114:1177-84. 2006
    ..Treatment for this disabling condition remains limited and largely ineffective. The goal of the present study was to investigate the therapeutic efficacy of hepatocyte growth factor (HGF) in animal models of lymphedema...
  77. pmc Splicing of a novel androgen receptor exon generates a constitutively active androgen receptor that mediates prostate cancer therapy resistance
    Scott M Dehm
    Department of Urology and Biochemistry, Mayo Clinical College of Medicine, Rochester, Minnesota, USA
    Cancer Res 68:5469-77. 2008
    ..Together, our data describe a simple and effective mechanism by which PCa cells can synthesize a constitutively active AR and thus circumvent androgen ablation...
  78. ncbi Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
    N Yang
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Nature 393:809-12. 1998
    ..Furthermore, insulin and an active form of Rac increase the activity of LIMK-1. Taken together, our results indicate that LIMK-1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphorylating cofilin...
  79. ncbi Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method
    Christopher J Tonkin
    Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Parkville, VIC 3010, Australia
    Mol Biochem Parasitol 137:13-21. 2004
    ..falciparum. In this paper, we present a novel series of P. falciparum transfection vectors based around the Gateway recombinatorial cloning system...
  80. ncbi Constitutive Stat3 activity up-regulates VEGF expression and tumor angiogenesis
    Guilian Niu
    Immunology Program, H Lee Moffitt Cancer Center and Research Institute, Department of Oncology, University of South Florida College of Medicine, Tampa, Florida, FL 33612, USA
    Oncogene 21:2000-8. 2002
    ..These studies provide evidence that the VEGF gene is regulated directly by Stat3 protein, and indicate that Stat3 represents a common molecular target for blocking angiogenesis induced by multiple signaling pathways in human cancers...
  81. pmc Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
    S Srinivas
    Department of Genetics and Development, Columbia University, New York, USA
    BMC Dev Biol 1:4. 2001
  82. pmc MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms
    Yan Zeng
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 100:9779-84. 2003
    ..These data suggest that miRNAs and siRNAs can use similar mechanisms to repress mRNA expression and that the choice of mechanism may be largely or entirely determined by the degree of complementary of the RNA target...
  83. pmc Mechanism of activation of protein kinase B by insulin and IGF-1
    D R Alessi
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, UK
    EMBO J 15:6541-51. 1996
    ..endogenous protein kinase B alpha (also known as RAC/Akt kinase) activity 12-fold in L6 myotubes, while after transfection into 293 cells PKBalpha was activated 20- and 50-fold in response to insulin and IGF-1 respectively...
  84. pmc MicroRNA-451 regulates LKB1/AMPK signaling and allows adaptation to metabolic stress in glioma cells
    Jakub Godlewski
    Dardinger Laboratory for Neuro oncology and Neurosciences, Department of Neurological Surgery, The Ohio State University Medical Center and James Comprehensive Cancer Center, Columbus, OH 43210, USA
    Mol Cell 37:620-32. 2010
    ..Thus, miR-451 is a regulator of the LKB1/AMPK pathway, and this may represent a fundamental mechanism that contributes to cellular adaptation in response to altered energy availability...
  85. pmc Reprogramming of murine and human somatic cells using a single polycistronic vector
    Bryce W Carey
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 106:157-62. 2009
    ..In addition we have generated human induced pluripotent stem (hiPS) cell lines from human keratinocytes, demonstrating that a single polycistronic virus can reprogram human somatic cells...
  86. ncbi MicroRNA-directed cleavage of HOXB8 mRNA
    Soraya Yekta
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 304:594-6. 2004
  87. ncbi Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency
    H Q Mao
    Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, 726 Ross Building, 720 Rutland Avenue, Baltimore, MD 21205, USA
    J Control Release 70:399-421. 2001
    ..The transfection efficiency of chitosan-DNA nanoparticles was cell-type dependent...
  88. pmc Human induced pluripotent stem cells free of vector and transgene sequences
    Junying Yu
    Morgridge Institute for Research, Madison, WI 53707 7365, USA
    Science 324:797-801. 2009
    ..These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells...
  89. ncbi Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133
    G A Gonzalez
    Clayton Foundation Laboratory for Peptide Biology, Salk Institute, La Jolla, California 92037
    Cell 59:675-80. 1989
  90. ncbi Transcriptional repression of the anti-apoptotic survivin gene by wild type p53
    William H Hoffman
    Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 277:3247-57. 2002
    ..The identification of survivin as a p53 repressed gene should aid in the elucidation of the contribution of transcriptional repression to p53-dependent apoptosis...
  91. ncbi MiR-203 controls proliferation, migration and invasive potential of prostate cancer cell lines
    Giuditta Viticchiè
    Department of experimental Medicine and Biochemical Sciences and Biochemistry Laboratory IDI IRCCS, University of Rome Tor Vergata, Rome, Italy
    Cell Cycle 10:1121-31. 2011
    ..Therefore, miR-203 could be a potentially new prognostic marker and therapeutic target in metastatic prostate cancer...
  92. ncbi A transcriptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyltransferase Tip60
    X Cao
    The Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9111 USA
    Science 293:115-20. 2001
    ..This complex potently stimulates transcription via heterologous Gal4- or LexA-DNA binding domains, suggesting that release of the cytoplasmic tail of APP by gamma-cleavage may function in gene expression...
  93. ncbi Inhibition of cell migration, spreading, and focal adhesions by tumor suppressor PTEN
    M Tamura
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892 4370, USA
    Science 280:1614-7. 1998
    ..Overexpression of FAK partially antagonized the effects of PTEN. Thus, PTEN phosphatase may function as a tumor suppressor by negatively regulating cell interactions with the extracellular matrix...
  94. ncbi Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity
    R Yan
    Cell and Molecular Biology, Pharmacia and Upjohn, Inc, Kalamazoo, MI 49007, USA
    Nature 402:533-7. 1999
    ..Thus, Asp2 is a new protein target for drugs that are designed to block the production of amyloid beta-peptide peptide and the consequent formation of amyloid plaque in Alzheimer's disease...
  95. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
    ..Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression...
  96. ncbi Phosphorylation of Bim-EL by Erk1/2 on serine 69 promotes its degradation via the proteasome pathway and regulates its proapoptotic function
    Frederic Luciano
    INSERM Unit 526, LNC Label, Faculte de Medecine, 28 Avenue de Valombrose, 06107 Nice Cedex 02, France
    Oncogene 22:6785-93. 2003
    ..Altogether, our findings demonstrate that phosphorylation of Bim-EL by Erk1/2 on serine 69 selectively leads to its proteasomal degradation and therefore represents a new and important mechanism of Bim regulation...
  97. pmc Regulation of synaptic structure and function by FMRP-associated microRNAs miR-125b and miR-132
    Dieter Edbauer
    The Picower Institute for Learning and Memory, Departments of Brain and Cognitive Sciences and Biology, Massachusetts Institute of Technology, Cambridge, MA 02319, USA
    Neuron 65:373-84. 2010
    ..Because NMDA receptor subunit composition profoundly affects synaptic plasticity, these observations have implications for the pathophysiology of fragile X syndrome, in which plasticity is altered...
  98. ncbi DISC1 (Disrupted-In-Schizophrenia 1) is a centrosome-associated protein that interacts with MAP1A, MIPT3, ATF4/5 and NUDEL: regulation and loss of interaction with mutation
    Jill A Morris
    Department of Neuroscience, Merck Research Laboratories, West Point, PA 19486, USA
    Hum Mol Genet 12:1591-608. 2003
  99. ncbi LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development
    Y Gong
    Cell 107:513-23. 2001
    ..These data indicate that Wnt-mediated signaling via LRP5 affects bone accrual during growth and is important for the establishment of peak bone mass...
  100. ncbi Transcriptional activation of miR-34a contributes to p53-mediated apoptosis
    Nina Raver-Shapira
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell 26:731-43. 2007
    ..Perturbation of miR-34a expression, as occurs in some human cancers, may thus contribute to tumorigenesis by attenuating p53-dependent apoptosis...
  101. pmc Specificity of microRNA target selection in translational repression
    John G Doench
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 18:504-11. 2004
    ..Thus, predicted miRNA:mRNA interactions must be viewed in the context of other potential interactions and cellular conditions...

Research Grants72

  1. HK polymers as transfection reagents for siRNA delivery
    Frank Xie; Fiscal Year: 2005
    We are proposing this project to develop and commercialize a novel, histidine/lysine (HK) polymer based, siRNA transfection reagent...
  2. NIH Director's Pioneer Award
    Junying Yuan; Fiscal Year: 2009
    ..No Abstract provided ..
  3. Regulation of HSV-1 Gene Expression and Replication by Nuclear Hormone Receptors
    SHAOCHUNG VICTOR HSIA; Fiscal Year: 2013
    ..1) TR binding, 2) NOR/SMRT recruitment, and 3) their functions on viral replication/release by transient transfection and viral infection in neuronal and non-neuronal cells. Specific Aim 2...
  4. Anti-tumor properties of curcumin in oral cancers: role of thioredoxin reductase
    STEPHEN WILLIAM TUTTLE; Fiscal Year: 2010
    ..Conversely, TxRd1 will be overexpressed in MSK-leuk1 cells by transfection with the full length wild type TxRd1 gene, including the selenocysteine insertion element, from the 3'UTR...
  5. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..We will use RNA interference and transient transfection methods to disrupt the spectrin cortical network...
  6. H1R Signaling and Immune Deviation in EAE
    Cory Teuscher; Fiscal Year: 2013
    ..In transfection studies, H1RR surface expression was substantially lower than H1RS, with H1RR exhibiting intracellular ..
  7. Vitamin D3, Interference with Androgen Signal and Inhibition of Prostate Cancer
    Bandana Chatterjee; Fiscal Year: 2013
    ..Methods: We will use Co-IP, chromatin IP, transfection, ShRNAs, inducible protein expression, lentivirus infection, cell proliferation, xenograft tumor growth in SCID ..
  8. Non-viral Genetic Modification of Antigen-presenting Cells in Allografts
    Wilson S Meng; Fiscal Year: 2011
    ..A polymeric system with low inflammatory potential will be used in ex vivo IL-10 gene transfection of skin transplants in mice...
  9. The Habenulo-Interpeduncular Pathway and Nicotine
    Christie D Fowler; Fiscal Year: 2013
    ..0) will be injected into select brain regions to permit transfection of cholinergic neurons/axons in BAC transgenic choline acetyltransferase (ChAT)::Cre mice...
  10. Skeletal Muscle. Ca Release Control Inside the Sarcoplasmic Reticulum.
    Eduardo Rios; Fiscal Year: 2013
    ..These goals are now feasible in living animals thanks to a DNA transfection method that works with every protein and can be used also to prevent their synthesis...
  11. Role and Regulation of INrf2
    ANIL KUMAR JAISWAL; Fiscal Year: 2013
    ..The domain deletion, transfection, immunoprecipitation, Western analysis, reporter and apoptosis assays will be used in these studies...
    Pi Wan Cheng; Fiscal Year: 2010
    ..These transcription factors will be identified by transfection with cDNAs of known transcription factors and pull-down with biotinylated promoter followed by assay with ..
  13. Hepatic Steatosis Modulation by Apo B Gene Transcription
    JANET DEHOFF SPARKS; Fiscal Year: 2010
    ..a potential mechanism of betaine action by assessment of global and promoter DNA hypomethylation and transient transfection assays using methylated promoter constructs...
  14. Molecular Mechanisms of Parvovirus Gene Expression and Replication
    Jianming Qiu; Fiscal Year: 2013
    ..microenvironment of EPCs in human bone marrow and fetal liver), and a reverse genetics approach that involves transfection of the B19V dsDNA-form genome into UT7/Epo-S1 cell line cells cultured under hypoxic conditions...
  15. Conference on Autonomic Regulation of Cardiovascular Function in Health and Disea
    Martin Frank; Fiscal Year: 2012
    ..J. Physiol. 296:R1-R8. 2009) that transfection of the subfornical organ with superoxide dismutase in mice with heart failure, not only reduces sympathetic ..
  16. Overcoming Temozolomide Resistance using MGMT-targeting Spherical Nucleic Acids
    TIMOTHY L SITA; Fiscal Year: 2013
    ..These particles possess unique characteristics that confer advantages over other gene transfection reagents, including (1) simultaneous transfection and gene regulation independent of auxiliary transfection ..
  17. Nuclear-Cytoplasmic Shuttling of Foxc2 via 14-3-3 Determines Proximal Tubule Cell
    DIANA LYNNE GOLDEN; Fiscal Year: 2012
    ..Inhibition of endogenous Foxc2 expression by siRNA transfection will be utilized to examine the effect of endogenous Foxc2 knockdown on [unreadable]-actinin localization...
    Raymond F Novak; Fiscal Year: 2010
    ..Transient transfection with miR-336 and -352 suppresses CYP2E1 mRNA levels...
  19. Stem Cell Homing for Treatment of Female Pelvic Floor Disorders
    Margot S Damaser; Fiscal Year: 2013 and will receive an infusion of MSCs in which homing cytokine receptors have been knocked down via transfection with small interfering RNA (siRNA)...
    Stephen J Lippard; Fiscal Year: 2013 modified nucleosomes in vitro and with platinated reporter plasmids in live cells following transfection. Repair shielding by high mobility group protein HMGB1 bound to Pt-DNA 1,2-intrastrand cross-links sensitizes ..
  21. Alcohol-Induced Bone Resorption: The Role of Oxidative Stress
    Martin J J Ronis; Fiscal Year: 2013
    ..NOX gain and loss of function studies will be performed by transient transfection and use of siRNA and the effects of hydrogen peroxide and free radical scavenging on the ERK/STAT3/RANKL ..
  22. In Vivo Function of TRAF6 As a Target of K63-Linked Polyubiquitination
    DEAN BALLARD; Fiscal Year: 2010
    ..ensure its correct spatial, temporal, and quantitative expression, which cannot be achieved via conventional transfection approaches...
  23. Regulation of insulins by the Rhox5 homeobox gene supports spermatogenesis
    James Maclean; Fiscal Year: 2009
    ..Transient transfection of Rhox5 expression plasmids increased the activity of Ins2 promoter-driven luciferase reporter constructs...
  24. Abnormal Bladder Epithelial Cell Gene Expression in BPS/IC
    SUSAN F KEAY; Fiscal Year: 2013
    ..BPS/IC cell phenotype will be confirmed via loss or gain of function experiments (including siRNA knockdown and transfection)...
  25. Regulation of apoptosis by PI 3-kinase/Akt signaling
    Geoffrey M Cooper; Fiscal Year: 2012
    ..RNA interference and transient transfection assays will be used to determine the roles of FOXO, NF[unreadable]B and Myc/Mad/Max transcription factors in PI ..
    Quentin J Baca; Fiscal Year: 2010
    ..At Harvard Mr. Baca did 2 rotations first in Thomas Look's lab working on making antibodies to oncogenic transfection factors involved in Human leukemias and then in Dr...
  27. The unique role of B-Raf in murine cardiac development and postnatal function
    Natasha N Chattergoon; Fiscal Year: 2013
    ..vascular smooth muscle cells (VSMC), protein analysis by western blot, immunostaining, adenoviral gene transfection, and primary bone marrow stromal cell characterization...
  28. microRNA mediation of Endothelial Progenitor Cell function in Myocardial Ichemia
    Prasanna Krishnamurthy; Fiscal Year: 2013
    ..MI) increased miR-377 expression in the myocardium, circulating EPCs and plasma, 3d post-MI;4) pre-miR-377 transfection in EPCs inhibits protein expression of STK35 (a novel kinase localized in the nucleus), a potential target gene ..
  29. Non-viral gene therapy for sickle cell anemia
    CLIFFORD JOHN STEER; Fiscal Year: 2012
    ..In the R21 exploratory phase, Specific Aim 1 is designed to optimize the Sleeping Beauty transposon (SB-Tn) and transfection efficiency in mouse bone marrow (BM) and hematopoietic stem cells (HSCs)...
    Sung Wan Kim; Fiscal Year: 2012
    ..Non-viral polymers are biocompatible and non-immunogenic so that little to no toxicity is present upon transfection or subsequent transplantation...
  31. Molecular Mechanisms of Gastrin Mediated Pathways in Gastric Cancer Cells
    Basabi Rana; Fiscal Year: 2013
    ..of technical approaches, including migration, invasion and cell proliferation assays, Western analyses, DNA transfection, luciferase and kinase assays, immunoprecipitation, SiRNA technology, subcutaneous xenografts and tail vein ..
    Clinton C MacDonald; Fiscal Year: 2013 look for germ cell-specific components, and test functions of DCstF-64 domains using a luciferase-based cell transfection assay and transgenic mice...
  33. The Role of the Nuclear Lamina in Chromatin Organization and Gene Expression in N
    Marcos Estecio; Fiscal Year: 2012 study the chromatin binding to the nuclear lamina (LAD-Seq) that is independent from engineered plasmids transfection and applicable to primary, uncultured cells...
  34. Inflammation and Repair in Cardiac Ischemia-Reperfusion
    Lewis C Becker; Fiscal Year: 2011
    ..molecular biology, gene expression profiling with cDNA arrays, gene transfer by recombinant adenoviral vectors, transfection of decoy oligonucleotides or interfering RNAs, use of bone marrow derived stromal cells, and use of mice with ..
  35. Neurotrophins and Neuropeptides in Colon and Bladder Hypersensitivity
    Liya Qiao; Fiscal Year: 2013 of drugs, behavioral studies and ex vivo/in vitro (DRG explants, isolated DRG neuron culture and transfection) systems...
  36. Novel High Affinity and Long-Acting Bovine FSH Analogs for Veterinary Superovulat
    BRUCE DALE WEINTRAUB; Fiscal Year: 2012
    ..potency/efficacy plus reduced potential for immunogenicity;initially express these novel analogs by transient transfection using a previously optimized, novel, high expression &low cost production method. 2...
  37. In vivo targeted gene silencing, a novel method
    WILLIAM ANTHONY TRUITT; Fiscal Year: 2012
    ..multitude of strategies to regulate gene expression and long-lasting effects on gene expression without viral transfection. However, PNAs are relatively impermeable to membranes, keeping in vivo uses to a minimum...
  38. The function and regulation of IL-33 in the airway epithelium in asthma
    ERIN D GORDON; Fiscal Year: 2013
    ..They will be ideal in advising me on cell biology, primary airway epithelial cell transfection, molecular cloning, biostatistics, and advanced human genetics...
  39. Epigenetic regulation of liver transporters
    Frederick J Suchy; Fiscal Year: 2013
    ..The integration, synergy and validity of identified components will be tested in liver cell transfection assays with promoters of FXR-regulated genes and by siRNA knockdown of individual coactivators and histone ..
    Miriam Falzon; Fiscal Year: 2009
    ..D regulate integrin a634 expression will be investigated using siRNAs and integrin promoter deletions/transient transfection assays...
    MARIA INES MORANO; Fiscal Year: 2013
    ..Using Originus'patented STEP (Surface Transfection and Expression Protocol) technology, these reporters were co-introduced with necessary transcription factors ..
  42. Roles of Hox genes in skin and hair follicle development
    Alexander Awgulewitsch; Fiscal Year: 2010
    ..Control elements will be defined by DNA binding, co-transfection and chromatin immunoprecipitation assays, and Hoxc13-dependent regulation in vivo using reporter gene analysis ..
  43. Improve Wound Healing with HIF-CA5 DNA Vector and Electroporation
    John W Harmon; Fiscal Year: 2010
    ..Using a research grade EP device, we have demonstrated sustained expression of HIF after CA5 transfection, resulting in up- regulation of mRNA expression for important angiogenic peptides including VEGF, PLGF, PDGF-B, ..
  44. Mechanisms of Arsenic-Induced Developmental Toxicity
    Lisa Bain; Fiscal Year: 2009
    ..Finally, methylation inducers and inhibitors, and transfection of methyltransferase genes will be used to determine whether the myogenic phenotype and myogenic protein ..
  45. Interaction of Estrogen and AMPK in the Hypothalamus
    Jessica Healy; Fiscal Year: 2012
    ..will be examined both in vitro and in vivo in the presence and absence of estradiol, utilizing cell culture, transfection, Western blotting, quantitative RT-PCR, ovariectomy and intracerebroventricular injection...
    EunDuck P Kay; Fiscal Year: 2010
    ..We will test the hypothesis in our in vitro EMT model using experimental protocols (transfection, multiplex protein array, GTP-pulldown assay, co-immunoprecipitation, two-dimensional gel electrophoresis, ..
  47. Genetic Analysis of Mosquito Metamorphosis
    James T Nishiura; Fiscal Year: 2012
    ..of other factors controlling mosquito growth and metamorphosis, we propose to develop in vivo transient transfection of mosquito larvae...
  48. Histone deacetylase 7 and its interacting proteins in endothelial cells
    Hung Ying Kao; Fiscal Year: 2013
    ..We will test our model using chromatin immunoprecipitation (ChIP), knockdown by siRNA, and transient transfection reporter assays to characterize the MCP-1 promoter...
  49. Hepatic Non-viral Gene Therapy
    Leaf Huang; Fiscal Year: 2013
    ..Galactose targeted LCP containing both a plasmid DNA and a decapeptide CR8C showed a high level of gene transfection in the liver hepatocytes of mice after tail vein injection in a non-hydrodynamic manner...
  50. Identification of the transcriptional regulators of chondrocyte hypertrophy
    ANDREW BRUCE LASSAR; Fiscal Year: 2013
    ..Consistent with this notion, the Lassar lab has established that co-transfection of ectopic Runx2 together with the BMP signal tranducer, Smad 1, can induce expression of a luciferase ..
  51. Polymorphisms of estrogen-metabolizing genes in kidney cancer
    Yuichiro Tanaka; Fiscal Year: 2013
    ..EMG wild-type plasmids by cloning and generation of polymorphic variants using site-directed mutagenesis, (b) Transfection of promoter region constructs into renal cell lines and determine their effects on gene expression by using a ..
  52. Targeted epigenetic therapy of triple-negative breast cancer
    Samuel Waxman; Fiscal Year: 2013
    ..Our data demonstrate that transfection of the minimal SID or treatment with the encoded 13 amino acid peptide (SID peptide) disrupts SID transcription ..
  53. Novel Approach to Oral Gene Therapy for Chronic Granulomatous Disease
    Peter E Newburger; Fiscal Year: 2010
    ..and cDNA expression in an in vitro system using elicited peritoneal phagocytes from Cybb knock-out mice and transfection with cDNA encoding gp91-phox for transient replacement of gene function and correction of the CGD phenotype...
  54. Proteolytic Pathways in Thrombus Resolution
    Toni M Antalis; Fiscal Year: 2013
    ..using ex vivo thrombolysis assays and cellular clot lysis assays, as well as cell culture models employing transfection and shRNA approaches. Biomechanical assays will assess vein wall injury during experimental DVT...
  55. Non-coding RNAs in the epigenetics of human centromere formation
    PETER WARBURTON; Fiscal Year: 2009
    ..Human centromeres contain highly repetitive alpha satellite DNA. Transfection of alpha satellite DNA into cells can result in de novo centromere and human artificial chromosome (HAC) ..
  56. Cellular Mechanisms of Lymphatic Muscle Contractility
    MICHAEL JOHN DAVIS; Fiscal Year: 2012
    ..These methods will be combined with short-term vessel culture and adenoviral transfection methods that allow protein overexpression or siRNA-mediated protein knockdown to change the expression of SM-B, ..
  57. Development of Ets2-Inhibitors
    DEV ARYA; Fiscal Year: 2009
    ..conjugates to target Ets2 promoter sequences that bind with high affinity and can be delivered without external transfection agents...
    Michael S Wolin; Fiscal Year: 2010
    ..arteries are studied with a combination of physiological function, molecular signaling, organ culture, transfection and metabolic modulation approaches to investigate mechanisms controlling sGC and its influence on vascular ..
  59. Emerging Functions of Mitochondrial Fission in Postischemic Endothelial Cells
    BARBARA RITA ALEVRIADOU; Fiscal Year: 2012
    ..b>Transfection with a lentivirus that expresses green fluorescent protein in mitochondria will be used to analyze their ..
  60. Toward suicidal automation of porphyric Leishmania for photodynamic vaccination
    Kwang Poo Chang; Fiscal Year: 2010
    ..We propose to improve the suicidal designs by rectifying the mutant deficiencies as follows: [1] Transfection of the porphyrinogenic mutants for phagolysosomal stage-specific expression of an additional enzyme to timely ..
  61. Targeting stress-mediated pathways in the treatment of muscle insulin resistance
    Paige C Geiger; Fiscal Year: 2013
    ..We will use both heat treatment and specific overexpression of HSPs via plasmid transfection to accomplish this aim...
  62. Mechanism of Renal Cell Injury in Diabetes
    Goutam Ghosh Choudhury; Fiscal Year: 2013
    ..aims, techniques including immunoprecipitation, immunoblotting, morphometry, immunohistochemistry, reporter transfection assays, chromatin immunoprecipitation assays, siRNA-mediated downregulation and conditional expression of ..
  63. Structure/Function of Steroid Transforming AKR's
    Trevor M Penning; Fiscal Year: 2011 catalyze the formation of 5[unreadable],3a-THS bile acid precursors using reconstituted systems in vitro, transfection studies in AKR null cells, and by phenotyping reactions in human hepatocytes...
  64. Regulation of Epithelial Sodium Channel by CFTR chloride channel
    Bakhrom Berdiev; Fiscal Year: 2009
    ..Our preliminary conventional co-IP has shown that untagged 2-ENaC interacts with untagged CFTR after transient transfection. Moreover, CFTR-ENaC association will be detected using the cell lines 16HBE14o- and LIM1864, which ..
    Beth Schachter; Fiscal Year: 1993
    ..Here, transfection studies will assess the relative potencies of the B-variant and wild type ER as transcriptional activators of ..
    Robert Glazer; Fiscal Year: 1993
    ..gene plays a pivotal role in myeloid differentiation based on studies in our laboratory demonstrating that transfection of immature myeloblast cell line K562 with the genomic c-fes gene results in the stable expression of clonal ..
  67. Multichannel Electrosonic Actuation Microarray for Cell-Based Screening
    JOHN MARK MEACHAM; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): High-throughput, parallel delivery and transfection of biologically relevant material into cells remains a difficult task...
    Pi Wan Cheng; Fiscal Year: 2000
    ..Hence, CF lungs have been targeted for gene therapy. Current gene therapy vectors suffer from low transfection efficiency or induction of host immune response, which preclude them from being used for gene therapy in vivo ..
  69. Gene Delivery of P53 in a Tumor-bearing Mouse Model
    ARCHIBALD MIXSON; Fiscal Year: 2007
    ..encoding antiangiogenic proteins, p53 gene therapy delivered by cationic liposomes is hampered by inadequate transfection in vivo...
  70. Dihydrodiol dehydrogenase mediates cisplatin resistance
    Henry Simpkins; Fiscal Year: 2007
    ..All four were upregulated by cisplatin treatment of the 2008 cells. Transfection experiments showed that forced overexpression of only DDH1 induced cisplatin/carboplatin resistance in the ..
  71. Lyn Kinase Regulation of FceRI-induced Mediator Release
    Becky Vonakis; Fiscal Year: 2003
    ..A variety of approaches, including peptide binding studies, yeast two-hybrid interaction and transfection of intact cells indicate that the unique domain of Lyn is associated with the Cterminus of the receptor's beta ..
    SANDRA MCLESKEY; Fiscal Year: 1999
    b>Transfection of MCF-7 breast carcinoma cells with fibroblast growth factor 4 (FGF-4), an angiogenic growth factor, produces a dramatic change in tumor phenotype when the cells are injected into the mammary fat pads of ovariectomized nude ..