dna fingerprinting

Summary

Summary: A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.

Top Publications

  1. pmc Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis
    Philip Supply
    INSERM U629, Institut Pasteur de Lille, 1, Rue du Prof Calmette, F 59019 Lille cedex, France
    J Clin Microbiol 44:4498-510. 2006
  2. ncbi A physical map of the 1-gigabase bread wheat chromosome 3B
    Etienne Paux
    Institut National de la Recherche Agronomique, Université Blaise Pascal INRA UBP, UMR 1095, Genetics Diversity and Ecophysiology of Cereals, Clermont Ferrand, France
    Science 322:101-4. 2008
  3. pmc Evaluation and strategy for use of MIRU-VNTRplus, a multifunctional database for online analysis of genotyping data and phylogenetic identification of Mycobacterium tuberculosis complex isolates
    Caroline Allix-Béguec
    Genoscreen, 1, rue du Professeur Calmette, Lille 59019 Cedex, France
    J Clin Microbiol 46:2692-9. 2008
  4. ncbi Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli
    M A Behr
    Department of Medicine, McGill University Health Centre, Montreal, Quebec, Canada
    Lancet 353:444-9. 1999
  5. ncbi Molecular epidemiology of tuberculosis
    Peter F Barnes
    Center for Pulmonary and Infectious Disease Control, Department of Medicine, University of Texas Health Center, Tyler, TX 75708 3154, USA
    N Engl J Med 349:1149-56. 2003
  6. pmc Genomic diversity among drug sensitive and multidrug resistant isolates of Mycobacterium tuberculosis with identical DNA fingerprints
    Stefan Niemann
    Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany
    PLoS ONE 4:e7407. 2009
  7. pmc A first generation BAC-based physical map of the rainbow trout genome
    Yniv Palti
    National Center for Cool and Cold Water Aquaculture, ARS USDA, Kearneysville, WV 25430, USA
    BMC Genomics 10:462. 2009
  8. ncbi HIV-1 and recurrence, relapse, and reinfection of tuberculosis after cure: a cohort study in South African mineworkers
    P Sonnenberg
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
    Lancet 358:1687-93. 2001
  9. ncbi Consistent treatment of length variants in the human mtDNA control region: a reappraisal
    H J Bandelt
    Department of Mathematics, University of Hamburg, Bundesstrasse 55, 20146, Hamburg, Germany
    Int J Legal Med 122:11-21. 2008
  10. ncbi A physical map of the chicken genome
    John W Wallis
    Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA
    Nature 432:761-4. 2004

Detail Information

Publications372 found, 100 shown here

  1. pmc Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis
    Philip Supply
    INSERM U629, Institut Pasteur de Lille, 1, Rue du Prof Calmette, F 59019 Lille cedex, France
    J Clin Microbiol 44:4498-510. 2006
    ..This 15-locus system is therefore proposed as the new standard for routine epidemiological discrimination of M. tuberculosis isolates and the 24-locus system as a high-resolution tool for phylogenetic studies...
  2. ncbi A physical map of the 1-gigabase bread wheat chromosome 3B
    Etienne Paux
    Institut National de la Recherche Agronomique, Université Blaise Pascal INRA UBP, UMR 1095, Genetics Diversity and Ecophysiology of Cereals, Clermont Ferrand, France
    Science 322:101-4. 2008
    ..This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach...
  3. pmc Evaluation and strategy for use of MIRU-VNTRplus, a multifunctional database for online analysis of genotyping data and phylogenetic identification of Mycobacterium tuberculosis complex isolates
    Caroline Allix-Béguec
    Genoscreen, 1, rue du Professeur Calmette, Lille 59019 Cedex, France
    J Clin Microbiol 46:2692-9. 2008
    ..The MIRU-VNTRplus database is a powerful tool for high-resolution clonal identification and has little equivalent in terms of functionalities among the bacterial genotyping databases available so far...
  4. ncbi Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli
    M A Behr
    Department of Medicine, McGill University Health Centre, Montreal, Quebec, Canada
    Lancet 353:444-9. 1999
    ..We aimed to estimate the risk of transmission from smear-negative individuals...
  5. ncbi Molecular epidemiology of tuberculosis
    Peter F Barnes
    Center for Pulmonary and Infectious Disease Control, Department of Medicine, University of Texas Health Center, Tyler, TX 75708 3154, USA
    N Engl J Med 349:1149-56. 2003
  6. pmc Genomic diversity among drug sensitive and multidrug resistant isolates of Mycobacterium tuberculosis with identical DNA fingerprints
    Stefan Niemann
    Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany
    PLoS ONE 4:e7407. 2009
    ..routine genotyping of clinical MTBC isolates for epidemiological purposes relies on highly discriminatory DNA fingerprinting methods based on mobile and repetitive genetic elements...
  7. pmc A first generation BAC-based physical map of the rainbow trout genome
    Yniv Palti
    National Center for Cool and Cold Water Aquaculture, ARS USDA, Kearneysville, WV 25430, USA
    BMC Genomics 10:462. 2009
    ..This resource will also facilitate efforts to obtain and assemble a whole-genome reference sequence for this species...
  8. ncbi HIV-1 and recurrence, relapse, and reinfection of tuberculosis after cure: a cohort study in South African mineworkers
    P Sonnenberg
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
    Lancet 358:1687-93. 2001
    ..We followed up a cohort of 326 South African mineworkers, who had successfully completed treatment for pulmonary tuberculosis in 1995, to determine the rate and mechanisms of recurrence...
  9. ncbi Consistent treatment of length variants in the human mtDNA control region: a reappraisal
    H J Bandelt
    Department of Mathematics, University of Hamburg, Bundesstrasse 55, 20146, Hamburg, Germany
    Int J Legal Med 122:11-21. 2008
    ..To remedy the situation, we propose a phylogenetic approach for multiple alignment and resulting notation...
  10. ncbi A physical map of the chicken genome
    John W Wallis
    Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA
    Nature 432:761-4. 2004
    ..This map represents approximately 91% of the chicken genome and enables identification of chicken clones aligned to positions in other sequenced genomes...
  11. ncbi Genetics and genomics of core short tandem repeat loci used in human identity testing
    John M Butler
    National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
    J Forensic Sci 51:253-65. 2006
    ..These core STR loci, which form the basis for DNA databases worldwide, will continue to play an important role in forensic science for many years to come...
  12. pmc The ins and outs of DNA fingerprinting the infectious fungi
    D R Soll
    Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
    Clin Microbiol Rev 13:332-70. 2000
    b>DNA fingerprinting methods have evolved as major tools in fungal epidemiology. However, no single method has emerged as the method of choice, and some methods perform better than others at different levels of resolution...
  13. pmc Comparison of DNA fingerprinting methods for use in investigation of type E botulism outbreaks in the Canadian Arctic
    Daniel Leclair
    Bureau of Microbial Hazards, Health Products and Food Branch, Food Directorate, Health Canada, Tunney s Pasture, PL 2204A2, Ottawa, Ontario, Canada K1A 0L2
    J Clin Microbiol 44:1635-44. 2006
    ..A modified PFGE protocol was judged to be the most useful method for typing epidemiologically related C. botulinum type E strains, based on its ability to type all strains reproducibly and with an adequate level of discrimination...
  14. ncbi Multiple-locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria
    Bjørn Arne Lindstedt
    Norwegian Institute of Public Health, Division for Infectious Diseases Control, Oslo, Norway
    Electrophoresis 26:2567-82. 2005
    b>DNA fingerprinting has attracted considerable interest as means for identifying, tracing and preventing the dissemination of infectious agents...
  15. pmc Illustration of a common framework for relating multiple typing methods by application to macrolide-resistant Streptococcus pyogenes
    J A Carriço
    Grupo de Biomatemática, Istituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande 6 2780 156 Oeiras, Portugal
    J Clin Microbiol 44:2524-32. 2006
    ..803 and 0.655, respectively). This was confirmed by the analysis of the larger data set available from http://spyogenes.mlst.net and underscores the necessity of performing PFGE or MLST to unambiguously define clones in S. pyogenes...
  16. ncbi Extended guidelines for mtDNA typing of population data in forensic science
    Walther Parson
    Institute of Legal Medicine, Innsbruck Medical University, Innsbruck, Austria
    Forensic Sci Int Genet 1:13-9. 2007
    ..The notation of sequence data should thus reflect this growing knowledge...
  17. ncbi Sampling strategy in molecular microbial ecology: influence of soil sample size on DNA fingerprinting analysis of fungal and bacterial communities
    Lionel Ranjard
    UMR de Microbiologie et Géochimie des sols, INRA CMSE, 17 rue de Sully, BP 86510, 21065 Dijon, France
    Environ Microbiol 5:1111-20. 2003
    ....
  18. ncbi Wide dissemination of OXA-23-producing carbapenem-resistant Acinetobacter baumannii clonal complex 22 in multiple cities of China
    Yiqi Fu
    Department of Respiratory Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, People s Republic of China
    J Antimicrob Chemother 65:644-50. 2010
    ..In this study, multilocus sequence typing (MLST) was used to describe the genetic backgrounds of carbapenem-resistant Acinetobacter baumannii (CRAB) and carbapenem-susceptible A. baumannii (CSAB) from multiple cities of China...
  19. pmc Determination of genotypic diversity of Mycobacterium avium subspecies from human and animal origins by mycobacterial interspersed repetitive-unit-variable-number tandem-repeat and IS1311 restriction fragment length polymorphism typing methods
    Nicolas Radomski
    Unité Zoonoses Bactériennes, LERPAZ, AFSSA, 23 Avenue du Général de Gaulle, Maisons Alfort F 94706 Cedex, France
    J Clin Microbiol 48:1026-34. 2010
    ..MIRU-VNTR typing therefore appears to be a convenient typing method capable of distinguishing the three main subspecies and strains of the complex and providing new epidemiological knowledge on MAC...
  20. ncbi Tuberculosis transmission by patients with smear-negative pulmonary tuberculosis in a large cohort in the Netherlands
    Alma Tostmann
    Department of Pulmonary Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Infect Dis 47:1135-42. 2008
    ..The objective of this study was to determine the proportion of TB transmission events caused by patients with smear-negative pulmonary TB in The Netherlands...
  21. pmc Inferring multiple refugia and phylogeographical patterns in Pinus massoniana based on nucleotide sequence variation and DNA fingerprinting
    Xue Jun Ge
    Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
    PLoS ONE 7:e43717. 2012
    ..In this study, we tested the central-marginal paradigm that predicts that the marginal populations tend to be less polymorphic than the central ones in their genetic composition, and examined a founders' effect in the island population...
  22. pmc DNA fingerprinting of the NCI-60 cell line panel
    Philip L Lorenzi
    Genomics and Bioinformatics Group, Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Mol Cancer Ther 8:713-24. 2009
    ..of cell line contamination or misidentification, quality control measures should routinely include DNA fingerprinting. We have, therefore, used standard DNA microsatellite short tandem repeats to profile the NCI-60, and the ..
  23. ncbi Genotyping of Mexican Cryptococcus neoformans and C. gattii isolates by PCR-fingerprinting
    L R Castañón Olivares
    Departamento de Microbiologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Distrito Federal, México C P 04510
    Med Mycol 47:713-21. 2009
    ....
  24. ncbi Experimental tests of the bacterial distance-decay relationship
    Thomas Bell
    Department of Zoology, University of Oxford, South Parks Road, Oxford, UK
    ISME J 4:1357-65. 2010
    ....
  25. ncbi Microsatellite and minisatellite markers based DNA fingerprinting and genetic diversity of blast and ufra resistant genotypes
    M A Latif
    Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    C R Biol 334:282-9. 2011
    ....
  26. ncbi How to get more out of molecular fingerprints: practical tools for microbial ecology
    Massimo Marzorati
    Laboratory for Microbial Ecology and Technology LabMET, Gent University, B9000 Gent, Belgium
    Environ Microbiol 10:1571-81. 2008
    ..These Rr, Dy and Fo values, each representing a score to describe a microbial community, can be plotted in a 3D graph. The latter represents a visual ecological interpretation of the initial raw fingerprinting pattern...
  27. ncbi DNA fingerprinting and new tools for fine-scale discrimination of Arabidopsis thaliana accessions
    Matthieu Simon
    Institut Jean Pierre Bourgin, UMR1318 INRA AgroParisTech, F 78000 Versailles, France
    Plant J 69:1094-101. 2012
    ..versailles.inra.fr/ijpb/crb/anatool) that provides a simple and efficient means to verify or determine the identity of A. thaliana accessions in any laboratory, without the need for any specific or expensive technology...
  28. ncbi DNA fingerprinting of Mycobacterium tuberculosis: from phage typing to whole-genome sequencing
    Anita C Schürch
    Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment RIVM, Centre for Infectious Disease Control CIb LIS, pb 22, P O Box 1, 3720 BA Bilthoven, The Netherlands
    Infect Genet Evol 12:602-9. 2012
    ..However, current DNA fingerprinting methods have important limitations...
  29. ncbi Cultivation-independent analysis of Pseudomonas species in soil and in the rhizosphere of field-grown Verticillium dahliae host plants
    Rodrigo Costa
    Federal Biological Research Centre for Agriculture and Forestry BBA, Messeweg 11 12, D 38104 Braunschweig, Germany
    Environ Microbiol 8:2136-49. 2006
    ..This report provides a better understanding of how different factors drive Pseudomonas community structure and diversity in bulk and rhizosphere soils...
  30. ncbi Analysis of rpsL and rrs mutations in Beijing and non-Beijing streptomycin-resistant Mycobacterium tuberculosis isolates from Singapore
    Y J Sun
    Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore City, Republic of Singapore
    Clin Microbiol Infect 16:287-9. 2010
    ..48-34.1). This is the first study to report the association of Beijing strains with the rpsL K43R mutation in STR-resistant M. tuberculosis isolates with de novo resistance, as determined by clustering analysis...
  31. ncbi iPBS: a universal method for DNA fingerprinting and retrotransposon isolation
    Ruslan Kalendar
    MTT BI Plant Genomics Laboratory, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, P O Box 65, Helsinki, Finland
    Theor Appl Genet 121:1419-30. 2010
    ..Furthermore, the inter-PBS amplification technique as such has proved to be a powerful DNA fingerprinting technology without the need for prior sequence knowledge.
  32. pmc Endemic and epidemic lineages of Escherichia coli that cause urinary tract infections
    Amee R Manges
    Department of Epidemiology, Biostatistics and Occupational Health, McGill University, Montreal, Quebec, Canada
    Emerg Infect Dis 14:1575-83. 2008
    ..Two clonal groups were identified in both locations. Epidemic transmission followed by endemic transmission of UTI-causing clonal groups may explain these clusters of UTI cases...
  33. ncbi Concordance and population studies along with stutter and peak height ratio analysis for the PowerPlex ® ESX 17 and ESI 17 Systems
    Carolyn R Hill
    National Institute of Standards and Technology, Chemical Science and Technology Laboratory, Gaithersburg, MD 20899 8312, USA
    Forensic Sci Int Genet 5:269-75. 2011
    ....
  34. ncbi The Mycobacterium tuberculosis Beijing genotype does not affect tuberculosis treatment failure in Vietnam
    Tran N Buu
    Pham Ngoc Thach Tuberculosis and Lung Disease Hospital, Ho Chi Minh City, Vietnam
    Clin Infect Dis 51:879-86. 2010
    ..We studied the effect of genotype on treatment failure in a rural area in Vietnam where multidrug resistance is strongly associated with the Beijing genotype...
  35. ncbi Forensic utilization of familial searches in DNA databases
    Cassandra J Gershaw
    John Jay College of Criminal Justice, North Hall, New York, NY 10019, USA
    Forensic Sci Int Genet 5:16-20. 2011
    ..This article discusses prospective positive outcomes of utilizing familial DNA searches and acknowledges potential negative outcomes, thereby presenting both sides of this very complicated, rapidly evolving situation...
  36. ncbi Low template STR typing: effect of replicate number and consensus method on genotyping reliability and DNA database search results
    Corina C G Benschop
    Netherlands Forensic Institute, Department of Human Biological Traces, P O Box 24044, 2490 AA The Hague, The Netherlands
    Forensic Sci Int Genet 5:316-28. 2011
    ..From the database searches follows the recommendation to specifically mark LT DNA profiles when entering them into the DNA database...
  37. ncbi Effective strategies for forensic analysis in the mitochondrial DNA coding region
    Michael D Coble
    Biotechnology Division, National Institute of Standards and Technology, 100Bureau Drive Mail Stop 8311, Gaithersburg, MD 20899, USA
    Int J Legal Med 120:27-32. 2006
    ..This is true even when only synonymous variation is targeted to minimize the potential for problems arising from discovery of mutations that have reportedly been related to disease...
  38. pmc MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation
    Dmitry Zubakov
    Department of Forensic Molecular Biology, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
    Int J Legal Med 124:217-26. 2010
    ..Additional work remains necessary in search for suitable miRNA markers for other forensically relevant body fluids...
  39. ncbi Risk factors for clustering of tuberculosis cases: a systematic review of population-based molecular epidemiology studies
    A Fok
    Center for Clinical Epidemiology and Evaluation, Vancouver, British Columbia, Canada
    Int J Tuberc Lung Dis 12:480-92. 2008
    ..Many molecular epidemiology studies have been conducted to identify risk factors for clustering of tuberculosis (TB) cases in the population...
  40. pmc Evaluation of a high-throughput repetitive-sequence-based PCR system for DNA fingerprinting of Mycobacterium tuberculosis and Mycobacterium avium complex strains
    Gerard A Cangelosi
    Seattle Biomedical Research Institute, Four Nickerson St, Suite 200, Seattle, WA 98107 1651, USA
    J Clin Microbiol 42:2685-93. 2004
    ..Rep-PCR also generated DNA fingerprints from M. intracellulare (n = 8) and MAC(x) (n = 2) strains. It shows promise as a fast, unified method for high-throughput genotypic fingerprinting of multiple Mycobacterium species...
  41. ncbi Haplotype diversity of 17 Y-chromosomal STRs in three native Sarawak populations (Iban, Bidayuh and Melanau) in East Malaysia
    Yuet Meng Chang
    Forensic DNA Serology Laboratory, Department of Chemistry, Petaling Jaya, Malaysia
    Forensic Sci Int Genet 3:e77-80. 2009
    ....
  42. pmc Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea
    Young Hwa Choi
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    FEMS Yeast Res 10:769-78. 2010
    ..This study affirms VNIc to be the most prevalent genotype of C. neoformans isolated from non-HIV patients with cryptococcosis...
  43. pmc Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat
    H Shaked
    Department of Plant Sciences, The Weizmann Institute of Science, Rehovot 76100, Israel
    Plant Cell 13:1749-59. 2001
    ....
  44. ncbi Utility of fast mycobacterial interspersed repetitive unit-variable number tandem repeat genotyping in clinical mycobacteriological analysis
    Caroline Allix
    Institut Pasteur de Bruxelles, Laboratoire Tuberculose et Mycobacteries, Brussels, Belgium
    Clin Infect Dis 39:783-9. 2004
    ..We describe its application to 3 typical clinical situations encountered in our laboratory (Institut Pasteur de Bruxelles, Laboratoire Tuberculose et Mycobacteries; Brussels, Belgium)...
  45. pmc Escherichia coli serotype O15:K52:H1 as a uropathogenic clone
    G Prats
    Departament de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma, 08025 Barcelona, Spain
    J Clin Microbiol 38:201-9. 2000
    ..These findings indicate that E. coli O15:K52:H1 constitutes a broadly distributed and clinically significant uropathogenic clone with fluid antimicrobial resistance capabilities...
  46. ncbi Genetic relationship in the 'Bacillus cereus group' by rep-PCR fingerprinting and sequencing of a Bacillus anthracis-specific rep-PCR fragment
    A Cherif
    Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita degli Studi, Via Celoria 2, 20133, Milano, Italy
    J Appl Microbiol 94:1108-19. 2003
    ..To evaluate the genetic relationship in the Bacillus cereus group by rep-PCR fingerprinting...
  47. ncbi Use of multiple markers in population-based molecular epidemiologic studies of tuberculosis
    J T Rhee
    Department of Health Research and Policy, Stanford University School of Medicine, California, USA
    Int J Tuberc Lung Dis 4:1111-9. 2000
    ..Fingerprinting with a secondary marker in strains with fewer than six IS6110-hybridizing bands enhances the tracking of strains, but its impact on population-level inferences has not been well studied...
  48. ncbi Molecular epidemiology of clinical and environmental isolates of the Cryptococcus neoformans species complex reveals a high genetic diversity and the presence of the molecular type VGII mating type a in Colombia
    Patricia Escandon
    Grupo de Microbiologia, Instituto Nacional de Salud, Bogota, Colombia
    FEMS Yeast Res 6:625-35. 2006
    ..The data presented form part of the Colombian contribution to the ongoing global survey of the C. neoformans species complex...
  49. ncbi Genome sequence of Vibrio splendidus: an abundant planctonic marine species with a large genotypic diversity
    Frédérique Le Roux
    Institut Pasteur, Unité Plasticité du Génome Bactérien, CNRS URA 2171, F 75015, Paris, France
    Environ Microbiol 11:1959-70. 2009
    ..Those results suggest that marine Vibrio can be a source of antibiotic resistance genes...
  50. ncbi A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex
    Kerry O'Donnell
    Microbial Genomics Research Unit, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA
    Fungal Genet Biol 46:936-48. 2009
    ..We also evaluated enniatin, fumonisin and moniliformin mycotoxin production in vitro within a phylogenetic framework...
  51. ncbi Effects of plant genotype and growth stage on the structure of bacterial communities associated with potato (Solanum tuberosum L.)
    Leo van Overbeek
    Plant Research International, Wageningen, The Netherlands
    FEMS Microbiol Ecol 64:283-96. 2008
    ..Its prevalence was affected by plant growth stage and experiment rather than by plant genotype. It was concluded that plant growth stage overwhelmed any effect of plant genotype on the bacterial communities associated with potato...
  52. ncbi Taxonomy. Will DNA bar codes breathe life into classification?
    Eliot Marshall
    Science 307:1037. 2005
  53. ncbi Population genetic analyses of the NGM STR loci
    Bruce Budowle
    Institute of Investigative Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Int J Legal Med 125:101-9. 2011
    ..108. Therefore, in contrast to the practices in calculating the rarity of a DNA profile, for kinship analyses independence between the loci, vWA and D12S391 cannot be assumed...
  54. pmc Molecular genetic identification of skeletal remains from the Second World War Konfin I mass grave in Slovenia
    Irena Zupanic Pajnic
    Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia
    Int J Legal Med 124:307-17. 2010
    ..The statistical analyses showed a high confidence of correct identification for all 28 victims in the Konfin I mass grave (posterior probability ranged from 99.9% to more than 99.999999%)...
  55. ncbi Validation of a 21-locus autosomal SNP multiplex for forensic identification purposes
    L A Dixon
    Research and Development, The Forensic Science Service, Solihull Parkway, Birmingham B37 7YN, UK
    Forensic Sci Int 154:62-77. 2005
    ..Reproducibility studies showed concordance between SNP profiles for different sample types, such as blood, saliva, semen and hairs, for the same individual, both within and between different DNA extracts...
  56. ncbi Evolution of the extensively drug-resistant F15/LAM4/KZN strain of Mycobacterium tuberculosis in KwaZulu-Natal, South Africa
    Manormoney Pillay
    Department of Medical Microbiology, Nelson R Mandela School of Medicine, University of KwaZulu Natal, South Africa
    Clin Infect Dis 45:1409-14. 2007
    ..The majority of the patients involved were infected with the same strain of Mycobacterium tuberculosis (F15/LAM4/KZN). We report this strain's development from multidrug resistance to XDR...
  57. pmc Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species
    Ming Cheng Luo
    Department of Plant Sciences, University of California, Davis, CA 95616, USA
    BMC Genomics 11:122. 2010
    ....
  58. pmc Contigs built with fingerprints, markers, and FPC V4.7
    C Soderlund
    Clemson University Genomic Institute, Clemson, South Carolina 29634 5808, USA
    Genome Res 10:1772-87. 2000
    ..This paper describes the new features in FPC, the scenario for building the maps of chromosomes 9, 10 and 13, and the results from the simulation...
  59. pmc Major Mycobacterium tuberculosis lineages associate with patient country of origin
    Michael B Reed
    Department of Medicine, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada
    J Clin Microbiol 47:1119-28. 2009
    ..We believe that this independent validation of the LSP method should encourage researchers to adopt this system in investigations aimed at elucidating the role of strain variation in TB...
  60. ncbi Almost forgotten or latest practice? AFLP applications, analyses and advances
    Heidi M Meudt
    Allan Wilson Centre for Molecular Ecology and Evolution, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand
    Trends Plant Sci 12:106-17. 2007
    Amplified fragment length polymorphism (AFLP) DNA fingerprinting is a firmly established molecular marker technique, with broad applications in population genetics, shallow phylogenetics, linkage mapping, parentage analyses, and single-..
  61. ncbi Interpreting low template DNA profiles
    David J Balding
    Department of Epidemiology and Public Health, Imperial College, St Mary s Campus, Norfolk Place, London W21PG, UK
    Forensic Sci Int Genet 4:1-10. 2009
    ..We illustrate that ignoring the possibility of drop-in is usually unfair to defendants, and argue that under circumstances in which the prosecution relies on drop-out, it may be unsatisfactory to ignore any possibility of drop-in...
  62. pmc Molecular epidemiology of multidrug-resistant Acinetobacter baumannii in a single institution over a 10-year period
    Naomi Runnegar
    Pathology Queensland Central Laboratory, Royal Brisbane and Women s Hospital, Brisbane, Queensland, Australia
    J Clin Microbiol 48:4051-6. 2010
    ..Antibiotic susceptibility within ST92 is variable, suggesting a role for mechanisms other than antibiotic resistance in its success...
  63. pmc Characterization of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus spp. isolated from US West Coast public marine beaches
    Olusegun O Soge
    Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA, USA
    J Antimicrob Chemother 64:1148-55. 2009
    ..MRCoNS) from marine water and intertidal beach sand from public beaches in Washington State, USA...
  64. ncbi Prevalence of genes encoding for members of the staphylococcal leukotoxin family among clinical isolates of Staphylococcus aureus
    Christof von Eiff
    Institute of Medical Microbiology, University of Munster, Hospital and Clinics, 48149 Munster, Germany
    Diagn Microbiol Infect Dis 49:157-62. 2004
    ..The co-possession of different virulence factors and their probable synergy should receive more attention in order to better understand their role in pathogenicity...
  65. ncbi Chemotherapy treatment in pediatric patients with acute myeloid leukemia receiving antimicrobial prophylaxis leads to a relative increase of colonization with potentially pathogenic bacteria in the gut
    Michel J van Vliet
    Department of Pediatric Oncology and Hematology, Beatrix Children s Hospital, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    Clin Infect Dis 49:262-70. 2009
    ....
  66. ncbi Characteristics of drug-resistant tuberculosis in Abkhazia (Georgia), a high-prevalence area in Eastern Europe
    Manuela Pardini
    Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanita, Rome, Italy
    Tuberculosis (Edinb) 89:317-24. 2009
    ....
  67. pmc Effects of plant genotype and growth stage on the betaproteobacterial communities associated with different potato cultivars in two fields
    Ozgul Inceoglu
    Department of Microbial Ecology, Centre for Ecological and Evolutionary Studies, University of Groningen, Kerklaan 30, Haren, Netherlands
    Appl Environ Microbiol 76:3675-84. 2010
    ..Interestingly, both cultivar type and plant growth stage affected the community under some circumstances...
  68. pmc Definition of the Beijing/W lineage of Mycobacterium tuberculosis on the basis of genetic markers
    Kristin Kremer
    Mycobacteria Reference Unit, Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, P O Box 1, 3720 BA Bilthoven, The Netherlands
    J Clin Microbiol 42:4040-9. 2004
    ..tuberculosis strains belonging to the Beijing/W lineage, as described in this work, will facilitate further studies on the spread and characterization of this widespread genotype family of M. tuberculosis strains...
  69. ncbi The evolution of DNA databases--recommendations for new European STR loci
    Peter Gill
    Forensic Science Service, Birmingham, UK
    Forensic Sci Int 156:242-4. 2006
    ..This will increase the number of European standard Interpol loci from 7 to 10...
  70. pmc Genetic diversity of Mycobacterium tuberculosis isolates from a tertiary care tuberculosis hospital in South Korea
    Isdore Chola Shamputa
    Tuberculosis Research Section, Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Microbiol 48:387-94. 2010
    ..tuberculosis Beijing strains. Within the study limits, our results also suggest that the problem of drug-resistant TB in the Republic of Korea may be largely due to acquired resistance as opposed to transmission...
  71. ncbi Antibiotic resistance of enterococci and related bacteria in an urban wastewater treatment plant
    Miguel Ferreira da Silva
    Escola Superior de Biotecnologia, Universidade Catolica Portuguesa, Porto, Portugal
    FEMS Microbiol Ecol 55:322-9. 2006
    ..A positive selection of ciprofloxacin-resistant enterococci was indicated by a significant increase in resistance prevalence (alpha<0.02) in treated wastewater compared with the raw wastewater...
  72. pmc Genotyping of genetically monomorphic bacteria: DNA sequencing in Mycobacterium tuberculosis highlights the limitations of current methodologies
    Iñaki Comas
    Division of Mycobacterial Research, Medical Research Council, National Institute for Medical Research, London, UK
    PLoS ONE 4:e7815. 2009
    ..Our findings have implications for strain typing in other genetically monomorphic bacteria...
  73. ncbi MtDNA SNP multiplexes for efficient inference of matrilineal genetic ancestry within Oceania
    Kaye N Ballantyne
    Department of Forensic Molecular Biology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Forensic Sci Int Genet 6:425-36. 2012
    ....
  74. ncbi Validation of the barcoding gene COI for use in forensic genetic species identification
    Nick Dawnay
    School of Biological Sciences, University of Wales, Bangor LL57 2UW, United Kingdom
    Forensic Sci Int 173:1-6. 2007
    ..A simulated case study compared the performance of COI and cytochrome b mtDNA genes. Findings are discussed in relation to the utility of the COI gene in forensic species identification...
  75. ncbi Widespread distribution of urinary tract infections caused by a multidrug-resistant Escherichia coli clonal group
    A R Manges
    Division of Epidemiology and Public Health Biology, School of Public Health, University of California at Berkeley, 94720, USA
    N Engl J Med 345:1007-13. 2001
    ..in a university community in California were evaluated for antibiotic susceptibility, O:H serotype, DNA fingerprinting, pulsed-field gel electrophoretic pattern, and virulence factors...
  76. pmc An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR ribotype
    Warren N Fawley
    Department of Microbiology, The General Infirmary, Old Medical School, Leeds LS1 3EX, United Kingdom
    J Clin Microbiol 49:4333-7. 2011
    ..These findings emphasize the value of enhanced fingerprinting to confirm or refute suspected CDI case clusters...
  77. pmc The emergence and importation of diverse genotypes of methicillin-resistant Staphylococcus aureus (MRSA) harboring the Panton-Valentine leukocidin gene (pvl) reveal that pvl is a poor marker for community-acquired MRSA strains in Ireland
    Angela S Rossney
    National MRSA Reference Laboratory, St James s Hospital, James s St, Dublin 8, Ireland
    J Clin Microbiol 45:2554-63. 2007
    ..3, and the remaining 11 isolates could not be subtyped. This study demonstrates that pvl is not a reliable marker for CA-MRSA in Ireland and reveals the emergence and importation of diverse genotypes of pvl-positive MRSA in Ireland...
  78. ncbi Metabolically active microbial communities of yellow and grey colonizations on the walls of Altamira Cave, Spain
    M C Portillo
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Avda Reina Mercedes, Sevilla, Spain
    J Appl Microbiol 104:681-91. 2008
    ..To determine the major components of total and metabolically active microbial communities of yellow and grey colonizations threatening the conservation of palaeolithic paintings in Altamira Cave (Spain)...
  79. pmc Overlapping population structures of nasal isolates of Staphylococcus aureus from healthy Dutch and American individuals
    Damian C Melles
    Erasmus MC, University Medical Center Rotterdam, room L 313, Department of Medical Microbiology and Infectious Diseases, s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands
    J Clin Microbiol 46:235-41. 2008
    ..In conclusion, the overall population structures of American and Dutch nasal carriage isolates of S. aureus are surprisingly similar, despite subtle geographic differences in the prevalence of certain S. aureus genotypes...
  80. ncbi A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers
    John M Butler
    Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA
    Forensic Sci Int 129:10-24. 2002
    ..Haplotype comparisons between the 20plex and a commercially available kit found excellent agreement across the 76 samples in the Y chromosome consortium panel...
  81. pmc TRiFLe, a program for in silico terminal restriction fragment length polymorphism analysis with user-defined sequence sets
    Pilar Junier
    Environmental Microbiology Laboratory, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Appl Environ Microbiol 74:6452-6. 2008
    ..TRiFLE was used for analyzing T-RFLP data generated for the amoA and pmoA genes. The peaks identified in the T-RFLP patterns show an overlap of ammonia- and methane-oxidizing bacteria in the metalimnion of a subtropical lake...
  82. pmc Development and validation of a comparative genomic fingerprinting method for high-resolution genotyping of Campylobacter jejuni
    Eduardo N Taboada
    Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, Canada
    J Clin Microbiol 50:788-97. 2012
    ..CGF40 is a high-resolution comparative genomics-based method for C. jejuni subtyping with high discriminatory power that is also rapid, low cost, and easily deployable for routine epidemiologic surveillance and outbreak investigations...
  83. ncbi Microbial fingerprinting detects unique bacterial communities in the faecal microbiota of rats with experimentally-induced colitis
    Ashis K Samanta
    National Institute of Animal Nutrition and Physiology, Bangalore, Karnataka, 560030, India
    J Microbiol 50:218-25. 2012
    ..Restoring microbial homeostasis by targeting colitis-associated OTU through specific microbiological interventions could form the basis of novel therapeutic strategies for IBD...
  84. ncbi Morphologic, genetic, and biochemical characterization of Helicobacter magdeburgensis, a novel species isolated from the intestine of laboratory mice
    Francisco Rivas Traverso
    Department of Medical Microbiology, Otto von Guericke University Magdeburg, D 39120 Magdeburg, Germany
    Helicobacter 15:403-15. 2010
    ..The aim of this study was to isolate and characterize a putative novel EHS that has previously been detected by PCR screening of specific-pathogen-free mice...
  85. ncbi Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic sperm injection: a novel, paired randomized controlled trial using DNA fingerprinting
    Eric J Forman
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Fertil Steril 98:644-9. 2012
    ..To assess the impact of oocyte vitrification on aneuploidy and reproductive potential by comparing vitrified and control oocytes from a single patient within a single cycle and a single fresh transfer...
  86. pmc Comparison of molecular typing methods useful for detecting clusters of Campylobacter jejuni and C. coli isolates through routine surveillance
    Clifford G Clark
    National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
    J Clin Microbiol 50:798-809. 2012
    ..Similarly, comparing several different typing methods reveals a great deal about differences in how the methods group individuals within the population...
  87. pmc Clusters of multidrug-resistant Mycobacterium tuberculosis cases, Europe
    Isabelle Devaux
    European Centre for Disease Prevention and Control, Stockholm, Sweden
    Emerg Infect Dis 15:1052-60. 2009
    ..Both clustering and the Beijing genotype were associated with strains originating in eastern European countries. Molecular cluster detection contributes to identification of transmission profile, risk factors, and control measures...
  88. pmc DNA fingerprinting techniques for the analysis of genetic and epigenetic alterations in colorectal cancer
    Johanna K Samuelsson
    Sanford Burnham Medical Research Institute SBMRI, 10901N Torrey Pines Rd, La Jolla, CA 92037, United States
    Mutat Res 693:61-76. 2010
    ..discuss approaches designed to analyze genetic and epigenetic alterations in colorectal cancer, especially DNA fingerprinting approaches to detect changes in DNA copy number and methylation...
  89. pmc A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes
    Rajat Aggarwal
    Department of Entomology, Purdue University, West Lafayette, IN 47907, USA
    BMC Genomics 10:293. 2009
    ..A physical map is needed to improve the ability to perform both positional cloning and comparative genomic analyses with the fully sequenced genomes of other dipteran species...
  90. ncbi Use of sibling pairs to determine the familial searching efficiency of forensic databases
    Thomas M Reid
    DNA Diagnostics Center, Fairfield, OH 45014, USA
    Forensic Sci Int Genet 2:340-2. 2008
    ....
  91. ncbi Antimicrobial resistance and genetic profiling of Escherichia coli from a commercial beef packing plant
    Mueen Aslam
    Agriculture and Agri Food Canada, Lacombe Research Centre, 6000 C and E Trail, Lacombe, Alberta, Canada
    J Food Prot 69:1508-13. 2006
    ..This stresses the need for improving hygienic conditions during all stages of commercial beef processing and meatpacking to avoid the risks of transfer of antimicrobial-resistant bacteria to humans...
  92. ncbi DNA-fingerprinting (AFLP and RFLP) for genotypic identification in species of the Pleurotus eryngii complex
    S Urbanelli
    Department of Genetics and Molecular Biology, University La Sapienza of Rome, Via dei Sardi 70, 00185, Rome, Italy
    Appl Microbiol Biotechnol 74:592-600. 2007
    ..AFLP also showed enough sensitivity to detect polymorphisms among the strains, proving to be an efficient DNA fingerprinting tool in studies of strain assignment...
  93. ncbi Developmental validation of reduced-size STR Miniplex primer sets
    Kerry L Opel
    International Forensic Research Institute, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA
    J Forensic Sci 52:1263-71. 2007
    ..Our results demonstrate that Miniplex STR amplification procedures are a robust and sensitive tool for the analysis of degraded DNA...
  94. ncbi Molecular phylogenetics and epidemiology of Candida albicans
    Frank C Odds
    Aberdeen Fungal Group, Institute of Medical Sciences, Aberdeen AB25 2ZD, UK
    Future Microbiol 5:67-79. 2010
    ..albicans strains, and resistance to flucytosine and terbinafine. There are also clade-related associations with lengths of tandem repeats in some cell-surface proteins, but not with virulence or type of infection...
  95. ncbi Microsatellite DNA fingerprinting, differentiation, and genetic relationships of clones, cultivars, and varieties of six poplar species from three sections of the genus Populus
    Muhammad H Rahman
    Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2H1, Canada
    Genome 45:1083-94. 2002
    ....
  96. pmc Genotypic and phenotypic relationships between clinical and environmental isolates of Stenotrophomonas maltophilia
    G Berg
    Department of Microbiology, University of Rostock, D 18055 Rostock, Germany
    J Clin Microbiol 37:3594-600. 1999
    ..Computer-assisted cluster analysis of the phenotypic and genotypic features did not reveal any clustering patterns for either clinical or environmental isolates...
  97. ncbi PCR-based DNA fingerprinting of Giardia duodenalis isolates using the intergenic rDNA spacer
    R M Hopkins
    WHO Collaborating Centre for the Molecular Epidemiology of Parasite Infections, School of Veterinary and Biomedical Studies, Murdoch University, Australia
    Parasitology 118:531-9. 1999
    ..IGS-PCR analysis was successfully applied to 11 Giardia cyst samples highlighting the potential for this approach to genotype Giardia isolates without the need for in vitro culture...
  98. ncbi Methodological problems in the molecular epidemiology of tuberculosis
    Megan Murray
    Department of Epidemiology, Harvard School of Public Health, Boston, MA 02115, USA
    Am J Epidemiol 155:565-71. 2002
    In systematic studies of the molecular epidemiology of tuberculosis, DNA fingerprinting is used to estimate the fraction of incident cases attributable to recent transmission of Mycobacterium tuberculosis rather than reactivation disease ..
  99. pmc Success rates of nuclear short tandem repeat typing from different skeletal elements
    Ana Milos
    International Commission on Missing Persons, Sarajevo, Bosnia and Herzegovina
    Croat Med J 48:486-93. 2007
    ....
  100. ncbi Forensim: an open-source initiative for the evaluation of statistical methods in forensic genetics
    Hinda Haned
    Universite de Lyon, Universite Lyon 1, CNRS, UMR 5558, Laboratoire de Biometrie et Biologie Evolutive, 69622 Villeurbanne, France
    Forensic Sci Int Genet 5:265-8. 2011
    ..Forensim is written in the R language and is freely available from http://forensim.r-forge.r-project.org. This paper presents an overview of the software's functionalities...
  101. ncbi The development of reduced size STR amplicons as tools for analysis of degraded DNA
    John M Butler
    Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8311, USA
    J Forensic Sci 48:1054-64. 2003
    ..These miniplex sets should prove valuable in the analysis of samples where allele dropout and reduced sensitivity of larger STR alleles occurs...

Research Grants62

  1. Oral Wash for Nucleic Acid Diagnosis and Prognosis of TB in HIV-Infected Ugandans
    JOHN LUCIAN DAVIS; Fiscal Year: 2012
    ..Public Health Impact: We are studying if fast new tests that do DNA fingerprinting on salt water mouthwashes are cheap and accurate for diagnosing tuberculosis in the lungs of patients with ..
  2. Hospital-Acquired Mupirocin-Resistant MRSA in Post-Partum Women and Newborns
    SHARON BETH BRODIE WRIGHT; Fiscal Year: 2013
    ..More recently CA-MRSA, as defined by pulsed-field gel electrophoresis, a form of "DNA fingerprinting", has become a growing cause of healthcare-acquired infection...
  3. Optically Patterned DNA Prism
    Kevin D Dorfman; Fiscal Year: 2012
    ..These devices will accelerate a number of key genomics applications, such as DNA fingerprinting of infectious organisms and genome assembly.
  4. Rational Engineering of Nanopost Arrays for DNA Electrophoresis
    Kevin D Dorfman; Fiscal Year: 2012
    ..generation sequencers may ultimately prove to be the most suitable method for re-sequencing projects, DNA fingerprinting and physical mapping will remain important for the assembly of unknown genomes and low- cost screening ..
  5. DISSEMINATED TUBERCULOSIS IN HIV INFECTION
    C von Reyn; Fiscal Year: 2004
    ..All isolates will have susceptibility tests and IS6110 DNA fingerprinting performed...
  6. Mulago Inpatient Noninvasive Diagnosis of Pneumocystis Pneumonia (MIND PCP) Study
    John Davis; Fiscal Year: 2007
    ..This study will test if DNA- fingerprinting of special mouthwashes can accurately diagnose HIV-related pneumonia, and save lives. [unreadable] [unreadable] [unreadable]..
  7. F.nucleatum in Amniotic Fluid Infection
    Yiping Han; Fiscal Year: 2004
    ..nucleatum will be isolated and identified to the subspecies level and differentiated by DNA fingerprinting. The results from this pilot study will enable subsequent investigation into the mechanism of infection, ..
  8. Development Modulation of Mouse Uterine Tumorigenesis
    Shuk Mei Ho; Fiscal Year: 2006
    ..In Aim 1,we will use a highly innovative technology (methylation-sensitive DNA fingerprinting or MS-AP-PCR) to identify genes that are hyper- or hypomethylated in neonatal and adult uteri from mice ..
  9. DNA Fingerprinting and Civil Liberties
    Benjamin Moulton; Fiscal Year: 2005
    ..abstract_text> ..
  10. MUTAGENICITY AND FITNESS IMPACTS OF ENVIRONMENTAL TOXINS
    SABINE LOEW; Fiscal Year: 2000
    ..This project is the first in a series to evaluate the use of DNA fingerprinting as a diagnostic molecular tool to detect significant exposure of wild mammals to environmental mutagens...
  11. HOST DEFENSE FACTORS IN ORAL ECOLOGY
    JOEL RUDNEY; Fiscal Year: 1991
    ..Restriction endonuclease chromosomal DNA fingerprinting and other molecular techniques will be used to characterize and compare streptococcal floras between ..
  12. IDENT OF H PYLORI VIRUL FACTORS IN ETHNIC POPULATIONS
    David Graham; Fiscal Year: 2000
    ..We also plan to use the DNA fingerprinting method, REP-PCR, to assess genetic similarity among Hp isolates within and between different ethnic groups...
  13. PAPILLARY SEROUS CARCINOMA OF THE PERITONEUM
    Samuel Mok; Fiscal Year: 2000
    ..sites using molecular genetic markers including X- chromosome inactivation, LOH, specific point mutation and DNA fingerprinting. These studies may serve to distinguish PSCP from epithelial ovarian cancer...
  14. Universal Fingerprinting Chips
    Kenneth Beattie; Fiscal Year: 2006
    ..One advantage of the UFC over traditional DNA fingerprinting methods is its greater information content, which is envisioned to increase the accuracy of species and ..
  15. GENOTYPING WITH MINOR-GROOVE-BINDER-CONTAINING PROBES.
    Michael Reed; Fiscal Year: 2003
    APPLICANT'S DESCRIPTION: DNA fingerprinting, or profiling, is widely used for paternity cases and criminal investigations (forensics). Current methodologies are cumbersome, expensive and often fail to produce interpretable results...
  16. MOLECULAR EPIDEMIOLOGY OF C ALBICANS IN AIDS PATIENTS
    Kaaren Vargas; Fiscal Year: 1999
    ..Using a newly developed automatic DNA fingerprinting system for assessing strain relatedness at the genetic level and several assays for assessment of virulence ..
  17. Single Cell DNA Fragment Sizing
    RICHARD KELLER; Fiscal Year: 2005
    ..Our approach eliminates the need for cell culturing common to other DNA fingerprinting methods, thereby reducing the analysis time from several days to hours...
  18. MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM AVIUM
    Vivek Kapur; Fiscal Year: 2000
    ..that may also be recovered from AIDS patients; and (iii) automated polymerase chain reaction (PCR)-based DNA fingerprinting strategies may be used for the rapid and unambiguous identification of M. avium clones...
  19. |
    MICHAEL DUIGNAN; Fiscal Year: 1991
    ..Such a detector will have a broad array of applications such as genetic testing and engineering, pharmaceutical purity testing, as well as forensic pathology and DNA fingerprinting.
  20. COMPARATIVE HYBRIDIZATION OF AP PCR ARRAYS
    SERGEI MALKHOSYAN; Fiscal Year: 2000
    DESCRIPTION: (Applicant's Description) We applied an unbiased DNA fingerprinting technique, the Arbitrarily-Primed PCR (AP-PCR) to study tumor- specific genetic changes...
  21. COMPARATIVE HYBRIDIZATION OF AP PCR ARRAYS
    SERGEI MALKHOSYAN; Fiscal Year: 2003
    DESCRIPTION: (Applicant's Description) We applied an unbiased DNA fingerprinting technique, the Arbitrarily-Primed PCR (AP-PCR) to study tumor- specific genetic changes...
  22. GENETICS AND INBREEDING IN NONHUMAN PRIMATES
    Bennett Dyke; Fiscal Year: 1993
    ..to our pedigree drawing package, and we will develop a pattern matching algorithm for analysis of DNA fingerprinting patterns...
  23. Microbial Fingerprinting Chip and Automated Analysis
    DON DALY; Fiscal Year: 2003
    b>DNA fingerprinting provides the capability to identify and genetically discriminate between closely related strains of microorganisms, which is required for forensic identification and tracking of disseminated bio-agents or pathogens (e...
  24. RAPID GENOTYPING WITH SHORT MINOR GROOVE PROBES
    Michael Singer; Fiscal Year: 1999
    b>DNA fingerprinting, or profiling, is widely used for paternity cases and criminal investigations (forensics). Current methodologies are cumbersome and often produce uninterpretable results...
  25. S AUREUS PATHOGENESIS IN HIV INFECTION AND IN DRUG USE
    FRANKLIN LOWY; Fiscal Year: 2002
    ..aureus infection. 2) Use DNA fingerprinting techniques to determine whether: strains that colonize also cause infection; carriage is clonal; ..
  26. Technology to Detect Genome wide DNA Methylation Changes
    Fumiichiro Yamamoto; Fiscal Year: 2002
    ..This PCR-based unbiased DNA fingerprinting technique permits the identification of the cleavage sites that exhibit DNA methylation alterations and ..
  27. DNA FINGERPRINTING OF PERIODONTAL BACTERIA
    Margaret Duncan; Fiscal Year: 1990
    This application describes feasibility study to extend and apply new DNA fingerprinting methods to 2 periodontal bacteria...
  28. INFECTIVITY OF PAUCIBACILLARY PULMONARY TUBERCULOSIS
    Peter Small; Fiscal Year: 2000
    ..The establishment of a TB molecular epidemiology lab (DNA fingerprinting, PCR and rapid drug susceptibility determination) in the Instituto Nacional de Diagnostico y Epidemiologicas ..
  29. ESTROGEN IMPRINTING OF PROSTATE GLAND VIA METHYLATON
    Gail Prins; Fiscal Year: 2005
    ..will perform differential methylation detection using a highly innovative technology (methylation-sensitive DNA fingerprinting or MS-AP-PCR) to identify genes that are hypermethylated in rat prostate glands developmentally exposed to ..
  30. STRUCTURE AND METABOLISM OF VASCULARIZED ALLOGRAFTS
    Victor Goldberg; Fiscal Year: 1993
    ..These analyses will establish a comprehensive perspective of the critical parameters determining the fate of a vascularized bone allograft. Specific protocols, based on this work, could be developed for useful clinical applications...
  31. PHYSIOLOGY, TERRITORIALITY AND DECISION MAKING
    GORDON ORIANS; Fiscal Year: 1993
    ..Lastly, DNA fingerprinting analysis will reveal the frequency of extra-pair fertilizations and the proportion of offspring sired by ..
  32. BIRMINGHAM SCIENCE EDUCATION PARTNERSHIP (BSEP) PHASE I
    Mary Williams; Fiscal Year: 2004
    ..to bring their classes to the GENEius program at McWane Science Center to conduct day-long experiments in DNA fingerprinting and the genetic basis for sickle cell anemia; 4) develop and build exhibits at McWane Center to help the ..
  33. SOMATIC GENETIC ALTERATIONS IN GASTROINTESTINAL CANCER
    Manuel Perucho; Fiscal Year: 2002
    ..analysis of losses and gains of chromosomal segments in gastrointestinal tumorigenesis by unbiased DNA fingerprinting. This method generates a profile of differences between tumor and normal genomes which we call "amplotype"...
  34. GORDON CONFERENCE ON STAPHYLOCOCCAL DISEASE
    George Stewart; Fiscal Year: 1991
    ..the human major histocompatibility complex, their pathophysiology, and regulation of their genes), surface properties of coagulase negative staphylococci, and molecular epidemiology (DNA probes, DNA fingerprinting of clinical isolates).
  35. MAGNETIC DISK ARRAY DNA ANALYZER
    Val Golovlev; Fiscal Year: 2001
    ..Due to fast speed analysis, it is very valuable for biomedical research. It also can be used for DNA fingerprinting for forensic applications as well as rapid microbial DNA analysis in field for biological weapon analysis...
  36. A SNP-BASED, HIGH-THROUGHPUT, LOW-COST GENOTYPING METHOD
    John Landers; Fiscal Year: 1999
    ..Additionally it offers a rapid, low- cost approach for digital DNA fingerprinting for military and criminal identification and forensic applications.
  37. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  38. SWITCHING IN THE ORAL COMMENSAL CANDIDA GLABRATA
    David Soll; Fiscal Year: 2006
    ..abstract_text> ..
  39. Molecular Epidemiological Approach to Determine S. mutans
    Yihong Li; Fiscal Year: 2005
    ..Application of the new molecular tools will have a substantial impact on improving the oral health of children. ..
  40. Switching and Candida Pathogenesis-A Molecular Analysis
    David Soll; Fiscal Year: 2005
    ..The first three aims have been formulated so that they provide several independent starting points for a reverse genetic approach for elucidating switch loci and the switching mechanism. ..
  41. Chronic Prostatitis Collaborative Research Network
    Anthony Schaeffer; Fiscal Year: 2007
    ..abstract_text> ..
  42. Prognosis in childhood ALL
    URSULA KEES; Fiscal Year: 2006
    ....
  43. Osteoprotegerin and osteopontin in aortic aneurysm
    Jonathan Golledge; Fiscal Year: 2009
    ..Understanding the role of these calcium-controlling proteins in artery weakness and how drug therapy can interfere in these effects is an important step in the development of new treatment for aortic aneurysm. ..
  44. Measuring Entomological Risk for Dengue
    THOMAS WALLACE SCOTT; Fiscal Year: 2010
    ..Our results will be relevant for dengue prevention programs based on vector control and/or vaccine strategies and will have immediate, practical applications for dengue surveillance and control worldwide. ..
  45. Quinolone resistant E coli in long term care facilities
    Ebbing Lautenbach; Fiscal Year: 2009
    ..abstract_text> ..
  46. MACROPHAGE IRON METABOLISM AND TUBERCULOSIS INFECTION
    Megan Murray; Fiscal Year: 2007
    ..tuberculosis may help identify those at high risk for disease progression and lead to therapeutic interventions aimed at reducing macrophage iron stores. [unreadable] [unreadable]..
  47. Clinical Impact of Quinolone-Resistant E.coli carriage
    Ebbing Lautenbach; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  48. Detection of Select Agents in Single-Well Assays
    David Alland; Fiscal Year: 2007
    ..3. To develop PCR assays that utilize "color-triplet coding" to uniquely label as many as 56 different species-specific molecular beacons. ..
  49. Detection of Pathogens in Avian Fecal Pollution
    SANDRA MCLELLAN; Fiscal Year: 2003
    ..abstract_text> ..
  50. MULTIPLE BLOOD FEEDING BY MOSQUITOES
    Thomas Scott; Fiscal Year: 2003
    ..We can reconstruct mosquito blood- feeding behavior with a DNA fingerprinting procedure that identifies the person(s) from which a mosquito took its meal(s)...
  51. Genetic Variability and Virulence of Human Adenovirus 7
    Adriana Kajon; Fiscal Year: 2005
    ..abstract_text> ..
  52. RCT OF STEPPING STONES BEHAVIOURAL INTERVENTION FOR HIV
    Rachel Jewkes; Fiscal Year: 2005
    ..It will also seek to understand what are the barriers to behavior change and what strategies are used by which people to circumnavigate these. ..
  53. Epigenetic Alterations in Homeotic Genes
    Fumiichiro Yamamoto; Fiscal Year: 2005
    ..Both the constitutive and inducible expression systems will be used to examine the tumor suppressor function of these homeotic genes. ..
  54. TUBERCULOSIS GENOTYPING AND EVOLUTIONARY CONSORTIUM
    David Alland; Fiscal Year: 2005
    ..3) To use the SNP-based phylogeny to investigate other analytic systems including IS6l 10 and VNTR-based DNA fingerprinting; and to provide insights into TB molecular epidemiology and evolution...
  55. Towards drugs that prevent resistance to the HIV OI, TB
    David Alland; Fiscal Year: 2006
    ..if embB306 mutations affect biosynthesis of LAM, capped LAM, arabinogalactan (AG) or other cell wall components in the presence and absence of EMB. ..
  56. Identification of Hypericum using Microscopy and DNA
    MATTHEW CIMINO; Fiscal Year: 2006
    ..Phase II aims are to expand the Phase I dataset for Hypericum and develop a working prototype assay for the detection, identification, and quantification for Hypericum species. [unreadable] [unreadable] [unreadable]..
  57. Congenital and neonatal malaria in Mali
    Abdoulaye Djimde; Fiscal Year: 2006
    ..research grant ..
  58. TGFBeta Signaling Mechanisms in the Pancreas
    Diane Simeone; Fiscal Year: 2003
    ..This grant proposal is submitted for the Small Grants Program for K08 Recipients-NIH K08 DK02637-01. ..