chromosome banding

Summary

Summary: Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.

Top Publications

  1. ncbi Chromosome territories, nuclear architecture and gene regulation in mammalian cells
    T Cremer
    Institute of Anthropology and Human Genetics, Ludwig Maximilians University, Richard Wagner Strasse 10, D 80333 Munich, Germany
    Nat Rev Genet 2:292-301. 2001
  2. ncbi Recent radiation in West African Taterillus (Rodentia, Gerbillinae): the concerted role of chromosome and climatic changes
    G Dobigny
    Museum national d histoire naturelle, Laboratoire Origine, Structure et Evolution de la Biodiversité, FRE CNRS 2695, 55, rue Buffon, Paris F75005, France
    Heredity (Edinb) 95:358-68. 2005
  3. ncbi Explosive chromosome evolution and speciation in the gerbil genus Taterillus (Rodentia, Gerbillinae): a case of two new cryptic species
    G Dobigny
    Laboratoire Mammifères et Oiseaux, Museum national d histoire naturelle, Paris, France
    Cytogenet Genome Res 96:117-24. 2002
  4. ncbi Distribution of (TTAGGG)n telomeric sequences in karyotypes of the Xenopus species complex
    I Nanda
    Department of Human Genetics, University of Wurzburg, Wurzburg, Germany
    Cytogenet Genome Res 122:396-400. 2008
  5. ncbi Comprehensive genetic characterization of CLL: a study on 506 cases analysed with chromosome banding analysis, interphase FISH, IgV(H) status and immunophenotyping
    C Haferlach
    MLL Munich Leukemia Laboratory, Munich, Germany
    Leukemia 21:2442-51. 2007
  6. ncbi A phylogenetic analysis using multidirectional chromosome painting of three species (Uroderma magnirostrum, U. bilobatum and Artibeus obscurus) of subfamily Stenodermatinae (Chiroptera-Phyllostomidae)
    J C Pieczarka
    Laboratorio de Citogenetica, ICB, UFPA, Belem, Brazil
    Chromosome Res 21:383-92. 2013
  7. ncbi Cytogenetic heteromorphisms: survey results and reporting practices of giemsa-band regions that we have pondered for years
    Arthur R Brothman
    Department of Pediatrics and Human Genetics, University of Utah School of Medicine, Salt Lake City, USA
    Arch Pathol Lab Med 130:947-9. 2006
  8. ncbi Chromosomal homologies among vampire bats revealed by chromosome painting (phyllostomidae, chiroptera)
    C G Sotero-Caio
    Laboratório de Genética e Citogenética Animal, Departamento de Genética CCB, Universidade Federal de Pernambuco, Recife, Brazil
    Cytogenet Genome Res 132:156-64. 2011
  9. ncbi Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes
    Constanze Karst
    Institute of Human Genetics and Anthropology, D 07743 Jena, Germany
    Int J Mol Med 17:209-13. 2006
  10. ncbi Chromosomal evolution in Rattini (Muridae, Rodentia)
    Daleen Badenhorst
    Evolutionary Genomics Group, Botany and Zoology Department, University of Stellenbosch, Private Bag X1, 7604 Matieland, South Africa
    Chromosome Res 19:709-27. 2011

Research Grants

  1. MOLECULAR AND SOMATIC CELL MAPPING OF DEER MOUSE GENES
    DUKE ROGERS; Fiscal Year: 1991
  2. PHYSICAL MAP OF HUMAN CHROMOSOME 18
    Uta Francke; Fiscal Year: 1993
  3. Development of Clinically Useful Single Copy FISH Probes
    PETER ROGAN; Fiscal Year: 2005
  4. MOLECULAR CYTOGENETIC ANALYSIS OF HUMAN CHROMOSOMES
    JAY GEORGE; Fiscal Year: 1992
  5. OBJECTIVE ANALYSIS OF CHROMOSOME BANDING PATTERNS
    Kenneth Castleman; Fiscal Year: 1999
  6. WAVELET ENHANCEMENT OF CHROMOSOME BANDING PATTERNS
    Qiang Wu; Fiscal Year: 2000
  7. E. COLI BASED VECTORS FOR GENE DELIVERY TO HUMAN CELLS
    PETER WARBURTON; Fiscal Year: 2002
  8. Chromosome Analysis of Single Cells
    Brynn Levy; Fiscal Year: 2007
  9. MOLECULAR GENETICS OF AUTISM
    Thomas Wassink; Fiscal Year: 2006
  10. Biacore T100
    Jeffrey Toretsky; Fiscal Year: 2006

Detail Information

Publications247 found, 100 shown here

  1. ncbi Chromosome territories, nuclear architecture and gene regulation in mammalian cells
    T Cremer
    Institute of Anthropology and Human Genetics, Ludwig Maximilians University, Richard Wagner Strasse 10, D 80333 Munich, Germany
    Nat Rev Genet 2:292-301. 2001
    ..This view is consistent with a topological model for gene regulation...
  2. ncbi Recent radiation in West African Taterillus (Rodentia, Gerbillinae): the concerted role of chromosome and climatic changes
    G Dobigny
    Museum national d histoire naturelle, Laboratoire Origine, Structure et Evolution de la Biodiversité, FRE CNRS 2695, 55, rue Buffon, Paris F75005, France
    Heredity (Edinb) 95:358-68. 2005
    ..This scenario would explain the current parapatric species distributions and their relationship to the West African hydrographic features...
  3. ncbi Explosive chromosome evolution and speciation in the gerbil genus Taterillus (Rodentia, Gerbillinae): a case of two new cryptic species
    G Dobigny
    Laboratoire Mammifères et Oiseaux, Museum national d histoire naturelle, Paris, France
    Cytogenet Genome Res 96:117-24. 2002
    ..Possible factors responsible for the rapid karyotypic evolution and speciation in this West African gerbil complex are discussed...
  4. ncbi Distribution of (TTAGGG)n telomeric sequences in karyotypes of the Xenopus species complex
    I Nanda
    Department of Human Genetics, University of Wurzburg, Wurzburg, Germany
    Cytogenet Genome Res 122:396-400. 2008
    ..clivii do not necessarily represent the relic of ancestral telomeres resulting from the fusion of chromosomes, but their occurrence is due to the fact that (TTAGGG)(n) repeat arrays may be a constituent of highly repetitive DNA...
  5. ncbi Comprehensive genetic characterization of CLL: a study on 506 cases analysed with chromosome banding analysis, interphase FISH, IgV(H) status and immunophenotyping
    C Haferlach
    MLL Munich Leukemia Laboratory, Munich, Germany
    Leukemia 21:2442-51. 2007
    In CLL data from chromosome banding analysis (CBA) have been scarce due to the low proliferative activity of CLL cells in vitro. We improved the cultivation technique using an immunostimulatory CpG-oligonucleotide DSP30 and IL-2...
  6. ncbi A phylogenetic analysis using multidirectional chromosome painting of three species (Uroderma magnirostrum, U. bilobatum and Artibeus obscurus) of subfamily Stenodermatinae (Chiroptera-Phyllostomidae)
    J C Pieczarka
    Laboratorio de Citogenetica, ICB, UFPA, Belem, Brazil
    Chromosome Res 21:383-92. 2013
    ..The genome-wide comparative maps based on chromosome painting and chromosome banding reveal the chromosome forms that characterize each taxonomic level within the Phyllostomidae and show the ..
  7. ncbi Cytogenetic heteromorphisms: survey results and reporting practices of giemsa-band regions that we have pondered for years
    Arthur R Brothman
    Department of Pediatrics and Human Genetics, University of Utah School of Medicine, Salt Lake City, USA
    Arch Pathol Lab Med 130:947-9. 2006
    ..Cytogenetic heteromorphisms (normal variants) pose diagnostic dilemmas. Common Giemsa-band heteromorphisms are not described in the literature, although Giemsa-banding is the method most frequently used in cytogenetic laboratories...
  8. ncbi Chromosomal homologies among vampire bats revealed by chromosome painting (phyllostomidae, chiroptera)
    C G Sotero-Caio
    Laboratório de Genética e Citogenética Animal, Departamento de Genética CCB, Universidade Federal de Pernambuco, Recife, Brazil
    Cytogenet Genome Res 132:156-64. 2011
    ..Karyotype comparisons allowed us to relate a number of conserved chromosomal segments among the 5 species, providing a broader database for understanding karyotype evolution in the family...
  9. ncbi Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes
    Constanze Karst
    Institute of Human Genetics and Anthropology, D 07743 Jena, Germany
    Int J Mol Med 17:209-13. 2006
    ..SKY revealed that the WMP2 cell line developed further four derivative chromosomes. After application of mcb five previously unrecognizable intrachromosomal rearrangements with 9 breakpoints were detected for the studied chromosomes...
  10. ncbi Chromosomal evolution in Rattini (Muridae, Rodentia)
    Daleen Badenhorst
    Evolutionary Genomics Group, Botany and Zoology Department, University of Stellenbosch, Private Bag X1, 7604 Matieland, South Africa
    Chromosome Res 19:709-27. 2011
    ..species representative of the genera Maxomys, Leopoldamys, Niviventer, Berylmys, Bandicota and Rattus using chromosome banding, cross-species painting (Zoo-fluorescent in situ hybridization or FISH) and BAC-FISH mapping...
  11. ncbi FISH-microdissection (FISH-MD) analysis of complex chromosome rearrangements
    J Weimer
    Oncology Laboratory, Gynecology and Obstetrics Clinic, Christian Albrechts University Kiel, Germany
    Cytogenet Cell Genet 88:114-8. 2000
    ..Rearranged chromosomes are first identified by multicolor-FISH, then the fluorophore-labeled derivative chromosomes are directly isolated by microdissection and reverse painted to identify the breakpoints...
  12. ncbi A comparative ZOO-FISH analysis in bats elucidates the phylogenetic relationships between Megachiroptera and five microchiropteran families
    M Volleth
    Institut fur Humangenetik, Universitat Erlangen Nurnberg, Erlangen, Germany
    Chromosome Res 10:477-97. 2002
    ..Therefore, for karyological reasons, monophyly of Chiroptera is strongly supported...
  13. ncbi Karyotype relationships of six bat species (Chiroptera, Vespertilionidae) from China revealed by chromosome painting and G-banding comparison
    L Ao
    Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, PR China
    Cytogenet Genome Res 115:145-53. 2006
    ..The integration of our maps with the published maps has enabled us to deduce chromosomal homologies between human and these six vesper bats and provided new insight into the karyotype evolution of the family Vespertilionidae...
  14. ncbi A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
    Jairo Campos
    Departamento de Zoologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, Brasil
    Mem Inst Oswaldo Cruz 98:387-90. 2003
    ..A simple technique should facilitate molecular cytogenetics used in the location of resistance and vector competence genes...
  15. ncbi Chromosome evolution and improved cytogenetic maps of the Y chromosome in cattle, zebu, river buffalo, sheep and goat
    G P Di Meo
    National Research Council CNR, ISPAAM, Laboratory of Animal Cytogenetics and Gene Mapping, Via Argine 1085, 80147, Naples, Italy
    Chromosome Res 13:349-55. 2005
    ....
  16. ncbi Karyotypic evolution and phylogenetic relationships in the order Chiroptera as revealed by G-banding comparison and chromosome painting
    Lei Ao
    Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, and Graduate School of the Chinese Academy of Sciences, Kunming, Yunnan, 650223, P R China
    Chromosome Res 15:257-67. 2007
    ..e. Pteropodiformes or Yinpterochiroptera) and thus improve our understanding on the karyotypic relationships and genome phylogeny of these bat species...
  17. ncbi Fragile sites in human and Macaca fascicularis chromosomes are breakpoints in chromosome evolution
    A Ruiz-Herrera
    Department de Biologia Cel lular i Fisiologia, Facultat de Medicina, Universitat Autonoma de Barcelona, Bellaterra, Spain
    Chromosome Res 10:33-44. 2002
    ..025) to the location of M. fascicularis fragile sites...
  18. ncbi PLAG1 gene alterations in salivary gland pleomorphic adenoma and carcinoma ex-pleomorphic adenoma: a combined study using chromosome banding, in situ hybridization and immunocytochemistry
    Carmo Martins
    Centro de Investigacao de Patobiologia Molecular CIPM, Instituto Portugues de Oncologia de Francisco Gentil, Lisboa, Portugal
    Mod Pathol 18:1048-55. 2005
    ....
  19. ncbi Comparative molecular cytogenetic analysis of three Leuciscus species (Pisces, Cyprinidae) using chromosome banding and FISH with rDNA
    Alicja Boron
    Department of Zoology, Faculty of Biology, University of Warmia and Mazury, M Oczapowskiego 5, Olsztyn, 10 718, Poland
    Genetica 135:199-207. 2009
    ..These NORs were CMA(3)-positive, GC-rich and C-positive heterochromatic sites in both species. Such chromosome banding features were also true for four NORs localizing on one of each SM and ST pair in L...
  20. pmc Molecular basis for expression of common and rare fragile sites
    Eitan Zlotorynski
    Department of Genetics, The Life Sciences Institute, The Hebrew University, Jerusalem, Israel 91904
    Mol Cell Biol 23:7143-51. 2003
    ....
  21. ncbi Reconstruction of the ancestral karyotype of eutherian mammals
    F Richard
    UMR 147 CNRS, Institut Curie, Section Recherche, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Chromosome Res 11:605-18. 2003
    ..Comparing chromosome banding with all ZOO-FISH data from literature or studied by us, this reconstruction can be proposed with only ..
  22. ncbi Comparative cytogenetics of bats (Chiroptera): the prevalence of Robertsonian translocations limits the power of chromosomal characters in resolving interfamily phylogenetic relationships
    Xiuguang Mao
    Kunming Institute of Zoology, Kunming, Yunnan, People s Republic of China
    Chromosome Res 16:155-70. 2008
    ..Both analyses clearly indicate the prevalence of homoplasic events that reduce the reliability of chromosomal characters for resolving interfamily relationships in bats...
  23. ncbi Chronic myeloid leukemia: a prospective comparison of interphase fluorescence in situ hybridization and chromosome banding analysis for the definition of complete cytogenetic response: a study of the GIMEMA CML WP
    Nicoletta Testoni
    Department of Hematology Oncology, L and A Seragnoli, University of Bologna and S Orsola Malpighi University Hospital, Bologna, Italy
    Blood 114:4939-43. 2009
    In chronic myeloid leukemia, different methods are available to monitor the response to therapy: chromosome banding analysis (CBA), interphase fluorescence in situ hybridization (I-FISH), and real-time quantitative polymerase chain ..
  24. ncbi Comparative chromosome painting of four Siberian Vespertilionidae species with Aselliscus stoliczkanus and human probes
    A I Kulemzina
    Molecular and Cellular Biology Department, Institute of Chemical Biology and Fundamental Medicine, SÐ RAS, Novosibirsk, Russia
    Cytogenet Genome Res 134:200-5. 2011
    ..auritus chromosome 16/17 using the HSA 16 probe explaining the different G-banding pattern in comparison to the homologous Myotis chromosome 16/17...
  25. ncbi Localization of the human CYP17 gene (cytochrome P450(17 alpha)) to 10q24.3 by fluorescence in situ hybridization and simultaneous chromosome banding
    Y S Fan
    Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada
    Genomics 14:1110-1. 1992
    ..3. This precise assignment may facilitate the understanding of the molecular basis of 17 alpha-hydroxylase/17,20-lyase deficiency and the evolution of the CYP superfamily of genes...
  26. ncbi High-resolution chromosome painting reveals the first genetic signature for the chiropteran suborder Pteropodiformes (Mammalia: Chiroptera)
    Marianne Volleth
    Department of Human Genetics, Otto von Guericke University, Leipziger Straße 44, 39120, Magdeburg, Germany
    Chromosome Res 19:507-19. 2011
    ..A subsequent survey across all major chiropteran families demonstrated that a paracentric inversion within this HSA 4 homologous segment could represent a synapomorphic character for the suborder Pteropodiformes...
  27. ncbi A girl with del(4)(q33) and occipital encephalocele: clinical description and molecular genetic characterization of a rare patient
    Roberto Quadrelli
    Instituto de Genetica Medica, Hospital Italiano, Montevideo, Uruguay
    Genet Test 11:4-10. 2007
    ..In particular, the prominent occipital encephalocele has not been observed before in a terminal 4q deletion...
  28. ncbi Chromosome evolution in domestic bovids as revealed by chromosome banding and FISH-mapping techniques
    L Iannuzzi
    National Research Council CNR, ISPAAM, Laboratory of Animal Cytogenetics and Gene Mapping, Naples, Italy
    Cytogenet Genome Res 126:49-62. 2009
    ..Evolution of the Bovinae-Caprinae autosomes and gonosomes is discussed on the basis of the most recent advances in chromosome banding, linkage studies, FISH-mapping and molecular information.
  29. ncbi A natural triploid in Trichomycterus davisi (Siluriformes, Trichomycteridae): mitotic and meiotic characterization by chromosome banding and synaptonemal complex analyses
    Luciana Andreia Borin
    Department of Cell Biology and Genetics, State University of Maringa, PR, Brazil
    Genetica 115:253-8. 2002
    ..Analysis of the synaptonemal complex (SC) by electronic microscopy shows that there is an incomplete pairing of the third set of chromosomes in the triploid individual...
  30. ncbi Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis
    Olga V Muravenko
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia
    Genetica 135:245-55. 2009
    ..Linum and Adenolinum, respectively. The obtained results suggest that species with 2n = 16, 2n = 18 and 2n = 30 originated from a 16-chromosome ancestor...
  31. ncbi Spectral karyotyping and interphase FISH reveal abnormalities not detected by conventional G-banding. Implications for treatment stratification of childhood acute lymphoblastic leukaemia: detailed analysis of 70 cases
    Ann Nordgren
    Department of Molecular Medicine, Karolinska Institutet, L8 02 Karolinska Hospital, SE 17176 Stockholm, Sweden
    Eur J Haematol 68:31-41. 2002
    ..Our results demonstrate the usefulness of SKY and interphase FISH for the identification of novel chromosome aberrations and cytogenetic abnormalities that provide prognostically important information in childhood ALL...
  32. ncbi Karyotype analysis of Lilium longiflorum and Lilium rubellum by chromosome banding and fluorescence in situ hybridisation
    K B Lim
    Plant Research International, Business Unit Genetics and Breeding, Wageningen, The Netherlands
    Genome 44:911-8. 2001
    ..Based on these techniques, we could identify most of chromosomes of the L. longiflorum and L. rubellum karyotypes...
  33. ncbi Karyotype reorganisation in the subtilis group of birch mice (Rodentia, Dipodidae, Sicista): unexpected taxonomic diversity within a limited distribution
    Y M Kovalskaya
    Severtzov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia
    Cytogenet Genome Res 132:271-88. 2011
    ..We conducted a comprehensive, comparative chromosome banding analysis of 52 birch mice (21 localities) referable to the subtilis group and report the presence of 5 ..
  34. ncbi Comparative chromosome painting defines the karyotypic relationships among the domestic dog, Chinese raccoon dog and Japanese raccoon dog
    Wenhui Nie
    Key Laboratory of Cellular and Molecular Evolution, The Chinese Academy of Sciences, Kunming, Yunnan, Peoples Republic of China
    Chromosome Res 11:735-40. 2003
    ..The large difference in chromosome number between the Chinese and Japanese raccoon dogs suggests that they should be considered as two distinct species...
  35. ncbi Comparison of karyotypes of Acipenser oxyrinchus and A. sturio by chromosome banding and fluorescent in situ hybridization
    Francesco Fontana
    Department of Biology, University of Ferrara, Via L Borsari 46, 44100 Ferrara, Italy
    Genetica 132:281-6. 2008
    ..oxyrinchus appear very similar from a cytogenetic point of view: these and molecular data repeatedly point out that A. sturio and A. oxyrinchus represent a sister clade in comparison to all other sturgeon species up to now studied...
  36. ncbi Localization by FISH of the 31 Texas nomenclature type I markers to both Q- and R-banded bovine chromosomes
    H Hayes
    Institut National de la Recherche Agronomique, INRA CRJ, Jouy en Josas, France
    Cytogenet Cell Genet 90:315-20. 2000
    ..Taking into account that cattle, goat and sheep have very similar banded karyotypes, the data presented here will help to establish a definite and complete reference chromosome nomenclature for these species...
  37. ncbi Chromosome banding in Amphibia. XXX. Karyotype aberrations in cultured fibroblast cells
    M Schmid
    Department of Human Genetics, Biocenter, University of Wurzburg, Wurzburg, Germany
    Cytogenet Genome Res 104:277-82. 2004
    ..holbrooki and G. riobambae are tandem fusions between two or more chromosomes. These tandem fusions originating in vitro in long-termed cell cultures reflect the chromosome mutations which also took place during amphibian phylogenesis...
  38. ncbi Systematics and phylogeny of West African gerbils of the genus Gerbilliscus (Muridae: Gerbillinae) inferred from comparative G- and C-banding chromosomal analyses
    V Volobouev
    Museum national d histoire naturelle, UMR 5202 Origine, Structure et Evolution de la Biodiversité, Paris, France
    Cytogenet Genome Res 116:269-81. 2007
    ....
  39. ncbi Karyotypic conservatism in the suborder Feliformia (Order Carnivora)
    P L Perelman
    Institute of Cytology and Genetics, Novosibirsk, Russia
    Cytogenet Genome Res 108:348-54. 2005
    ....
  40. ncbi The ancestral carnivore karyotype (2n = 38) lives today in ringtails
    William G Nash
    H and W Cytogenetic Services, Inc, Lovettsville, VA 20180, USA
    J Hered 99:241-53. 2008
    ..Banded chromosomes of the ACK are used to evaluate the consistency between recently determined molecular phylogenetic relationships and postulated cytogenetic dynamics in the same Carnivore species...
  41. ncbi Unusually extensive karyotype reorganization in four congeneric Gerbillus species (Muridae: Gerbillinae)
    V M Aniskin
    Severtsov Institut of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia
    Cytogenet Genome Res 112:131-40. 2006
    ..Based on the chromosomal data obtained here, the systematic and geographic implications for these North African species are also discussed...
  42. ncbi Characterization of diploid, tetraploid and hexaploid Helianthus species by chromosome banding and FISH with 45S rDNA probe
    André L L Vanzela
    Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina, PR, Brazil
    Genetica 114:105-11. 2002
    ..Karyomorphological differences include variation in number, size and chromosome morphology, suggesting that rearrangements involving small heterochromatic and rDNA segments played a major role in karyotype evolution...
  43. ncbi Chromosome banding in three species of Hypsiboas (Hylidae, Hylinae), with special reference to a new case of B-chromosome in anuran frogs and to the reduction of the diploid number of 2n = 24 to 2n = 22 in the genus
    Simone Lilian Gruber
    Departamento de Biologia, Instituto de Biociencias, Universidade Estadual Paulista UNESP, Av 24A, 1515, 13506 900 Rio Claro, SP, Brasil
    Genetica 130:281-91. 2007
    ....
  44. pmc Consensus statement: chromosomal microarray is a first-tier clinical diagnostic test for individuals with developmental disabilities or congenital anomalies
    David T Miller
    Division of Genetics and Department of Laboratory Medicine, Children s Hospital Boston and Harvard Medical School, Boston, MA, USA
    Am J Hum Genet 86:749-64. 2010
    ..G-banded karyotype analysis should be reserved for patients with obvious chromosomal syndromes (e.g., Down syndrome), a family history of chromosomal rearrangement, or a history of multiple miscarriages...
  45. pmc Evaluation of breast cancer polyclonality by combined chromosome banding and comparative genomic hybridization analysis
    M R Teixeira
    Department of Genetics, Portuguese Oncology Institute, Porto, Portugal
    Neoplasia 3:204-14. 2001
    Cytogenetically unrelated clones have been detected by chromosome banding analysis in many breast carcinomas...
  46. ncbi Diversity in the origins of sex chromosomes in anurans inferred from comparative mapping of sexual differentiation genes for three species of the Raninae and Xenopodinae
    Yoshinobu Uno
    Laboratory of Animal Cytogenetics, Biosystems Science Course, Graduate School of Life Science, Hokkaido University, Sapporo, 060 0810, Japan
    Chromosome Res 16:999-1011. 2008
    ..laevis and R. rugosa. Cyp17, Cyp19, Dmrt1, Sox9, and WT1 were localized to autosomes in X. laevis and R. rugosa, suggesting that these five genes probably are not candidates for the sex-determining genes in the two anuran species...
  47. ncbi Protocols for cytogenetic studies of human embryonic stem cells
    Lorraine Faxon Meisner
    Department of Population Health Sciences, University of Wisconsin, and Cell Line Genetics, LLC, 510 Charmany Drive, Suite 254, Madison, WI 53719, USA
    Methods 45:133-41. 2008
    ..The following discussion describes the cytogenetic methods used in our laboratory to study cultured hESCs, along with recommendations for integrating these methods into a plan for routine cell line quality control...
  48. ncbi The pattern of phylogenomic evolution of the Canidae
    W G Nash
    H and W Cytogenetic Services, Inc, Lovettsville, VA, USA
    Cytogenet Cell Genet 95:210-24. 2001
    ..The results indicate an ancestral episode of extensive centric fission leading to an ancestral canid genome organization that was subsequently reorganized by multiple chromosome fusion events in some but not all Canidae lineages...
  49. ncbi Multicolor chromosome banding (MCB) with YAC/BAC-based probes and region-specific microdissection DNA libraries
    T Liehr
    Institute of Human Genetics and Anthropology, University of Jena, Jena, Germany
    Cytogenet Genome Res 97:43-50. 2002
    Multicolor chromosome banding (MCB) allows the delineation of chromosomal regions with a resolution of a few megabasepairs, i.e., slightly below the size of most visible chromosome bands...
  50. ncbi Characterization of a wheat-Thinopyrum bessarabicum (T2JS-2BS.2BL) translocation line
    Zengjun Qi
    National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China
    Theor Appl Genet 121:589-97. 2010
    ..In addition, four markers specific for the translocated segment were identified, which can be used for marker-aided screening...
  51. ncbi Chromosome evolution in three Brazilian Leptodactylus species (Anura, Leptodactylidae), with phylogenetic considerations
    João Reinaldo Cruz Campos
    Instituto de Biociencias, Univ Estadual Paulista, UNESP, Rio Claro, SP, Brasil
    Hereditas 146:104-11. 2009
    ..The data obtained here are in accordance with the proposed phylogeny to the genus, and the chromosomal analyses in these Leptodactylus showed that the karyotype evolution was based mainly in centric fusion and pericentric inversion...
  52. pmc Molecular definition of high-resolution multicolor banding probes: first within the human DNA sequence anchored FISH banding probe set
    Anja Weise
    Institute of Human Genetics and Anthropology, D 07740 Jena, Germany
    J Histochem Cytochem 56:487-93. 2008
    ..In summary, the unique possibilities of the MCB technique to characterize chromosomal breakpoints in one FISH experiment are now complemented by the feature of being anchored within the human DNA sequence at the BAC level...
  53. ncbi Cryptic duplication of 12q24.33 --> qter in a child with Angelman syndrome-simultaneous occurrence of two unrelated cytogenetic events
    M Sathanoori
    Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, PA, USA
    Am J Med Genet A 143:985-94. 2007
    ..We compare our case with the published cases, and discuss the implications of our findings and its relevance in addressing genetic counseling issues...
  54. ncbi Lissencephaly with der(17)t(17;20)(p13.3;p12.2)mat
    Mary Ann Thomas
    F Clarke Fraser Clinical Genetics Unit, Division of Medical Genetics, Department of Pediatrics, Montreal Children s Hospital, Montreal, Quebec, Canada
    Am J Med Genet A 124:292-5. 2004
    ..This report may contribute to the delineation of a phenotype resulting from partial monosomy 17p and partial trisomy of 20p...
  55. ncbi Overrepresentation of small supernumerary marker chromosomes (sSMC) from chromosome 6 origin in cases with multiple sSMC
    Thomas Liehr
    Institut fur Humangenetik und Anthropologie, D 07740 Jena, Germany
    Am J Med Genet A 140:46-51. 2006
    ..1 approximately 12 --> q10::q10 --> p11.1 approximately 12::), respectively. Reversed array CGH using the DNA of the microdissected sSMC as probe confirmed the FISH results and enabled the rapid mapping of the breakpoints...
  56. ncbi Multitude multicolor chromosome banding (mMCB) - a comprehensive one-step multicolor FISH banding method
    A Weise
    Institute of Human Genetics and Anthropology, University of Jena, Jena, Germany
    Cytogenet Genome Res 103:34-9. 2003
    Multicolor chromosome banding (MCB) using one single chromosome-specific MCB probe set per experiment was previously reported as powerful tool in molecular cytogenetics for the characterization of all kinds of human marker chromosomes...
  57. ncbi Microdissection-derived murine mcb probes from somatic cell hybrids
    Vladimir Trifonov
    Institut für Human Genetics and Anthropology, Postfach, D 07740 Jena, Germany
    J Histochem Cytochem 53:791-2. 2005
    ..During creation of the mcb-probes, the latter enabled an unambiguous identification of the, otherwise in GTG-banding, hardly distinguishable murine chromosomes...
  58. ncbi Cytogenetic aberrations in spontaneous endometrial adenocarcinomas in the BDII rat model as revealed by chromosome banding and comparative genome hybridization
    Ahmad Hamta
    CMB Genetics, Lundberg Laboratory, Goteborg University, Box 462, SE 40530, Gothenburg, Sweden
    Cancer Genet Cytogenet 159:123-8. 2005
    ....
  59. ncbi Comparative chromosome banding analysis of three South American species of rice rats of the genus Oryzomys (Rodentia, Sigmodontinae)
    V T Volobouev
    Laboratoire de Zoologie, Mammifères et Oiseaux, Museum national d histoire naturelle, Paris, France
    Chromosome Res 8:295-304. 2000
    ..In the light of available chromosomal and molecular data, the significance of intra- and interspecies karyotypic variability within Oryzomys and its relevance to systematics and phylogeny of the genus are discussed...
  60. ncbi Evolutionary conserved chromosomal segments in the human karyotype are bounded by unstable chromosome bands
    A Ruiz-Herrera
    Departament de Biologia Cellular, Fisiologia i Immunologia, Universitat Autonoma de Barcelona, Spain
    Cytogenet Genome Res 108:161-74. 2005
    ....
  61. ncbi Evolution of genome organizations of squirrels (Sciuridae) revealed by cross-species chromosome painting
    Tangliang Li
    Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, People s Republic of China
    Chromosome Res 12:317-35. 2004
    ..The superorder Glires (orde Rodentia + order Lagomorpha) is firmly supported by two conserved syntenic associations between human chromosomes 1 and 10p homologues, and between 9 and 11 homologues...
  62. ncbi The DAPI banded karyotype of the domestic dog (Canis familiaris) generated using chromosome-specific paint probes
    M Breen
    Animal Health Trust, Kentford, Suffolk, UK
    Chromosome Res 7:401-6. 1999
    ..These data will facilitate the accurate assignment of FISH-mapped loci to all chromosomes comprising the karyotype and form the basis for an agreed standard of the dog karyotype...
  63. ncbi Zoo-FISH analysis: the American mink (Mustela vison) closely resembles the cat karyotype
    H Hameister
    Abteilung Medizinische Genetik, Universitat Ulm
    Chromosome Res 5:5-11. 1997
    ..The mink arrangement proved to be very similar to the arrangement found in the cat, thus corroborating the well-known high karyotype conservation described for Feloidea...
  64. pmc Detection of submicroscopic deletions in band 17p13 in patients with the Miller-Dieker syndrome
    C E Schwartz
    Greenwood Genetic Center, SC 29646
    Am J Hum Genet 43:597-604. 1988
    ..The most distal locus, defined by p144D6, was present in one MDS patient, possibly defining the distal limits of the MDS region in band 17p13.3. None of these loci were absent in one case of lissencephaly without MDS...
  65. ncbi Microdissection based high resolution multicolor banding for all 24 human chromosomes
    Thomas Liehr
    Institute of Human Genetics and Anthropology, Kollegiengasse 10, D 07740 Jena, Germany
    Int J Mol Med 9:335-9. 2002
    ..In conclusion, the MCB-technique is a high resolution alternative to other FISH based chromosome banding approaches and suited to clarify, which changes appeared in complex chromosomal rearrangements.
  66. ncbi Amplification of AML1 in childhood acute lymphoblastic leukemias
    P Dal Cin
    Department of Pathology, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Genes Chromosomes Cancer 30:407-9. 2001
    ..It remains to be elucidated whether this amplification results in up-regulation of the normal AML1 gene product or a potentially mutant AML1 transcript...
  67. ncbi Comparative FISH mapping in river buffalo and sheep chromosomes: assignment of forty autosomal type I loci from sixteen human chromosomes
    L Iannuzzi
    National Research Council CNR, Laboratory of Animal Cytogenetics and Gene Mapping, IABBAM, Naples Italy
    Cytogenet Cell Genet 94:43-8. 2001
    ....
  68. ncbi Comparison of chromosome banding analysis, interphase- and hypermetaphase-FISH, qualitative and quantitative PCR for diagnosis and for follow-up in chronic myeloid leukemia: a study on 350 cases
    C Schoch
    Department of Internal Medicine III, University Hospital Grosshadern, Ludwig Maximilians University, Munich, Germany
    Leukemia 16:53-9. 2002
    ..b>Chromosome banding analysis (CA) is still the gold standard but other techniques like Southern blot, fluorescence in situ ..
  69. ncbi Low rate of genomic repatterning in Xenarthra inferred from chromosome painting data
    G Dobigny
    Evolutionary Genomics Group, Department of Botany and Zoology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
    Chromosome Res 13:651-63. 2005
    ..Finally, our results provide a glimpse of a likely ancestral karyotype for the extant Xenarthra, a pivotal group for understanding eutherian genome evolution...
  70. pmc Reciprocal chromosome painting among human, aardvark, and elephant (superorder Afrotheria) reveals the likely eutherian ancestral karyotype
    F Yang
    Centre for Veterinary Science, University of Cambridge, Cambridge CB3 0ES, United Kingdom
    Proc Natl Acad Sci U S A 100:1062-6. 2003
    ....
  71. ncbi Comparison of the human with the sheep genomes by use of human chromosome-specific painting probes
    L Iannuzzi
    National Research Council, I A B B A M, Via Argine 1085, 80147 Naples, Italy
    Mamm Genome 10:719-23. 1999
    ..A total of 48 human chromosome segments were detected in sheep chromosomes. Comparisons with sheep gene mapping data available and previous Zoo-FISH data obtained in sheep, cattle, and river buffalo were performed...
  72. ncbi Cytogenetic analysis of the neotropical spider Nephilengys cruentata (Araneomorphae, Tetragnathidae): standard staining nors, C-bands and base-specific fluorochromes
    D Araujo
    Universidade Estadual Paulista, UNESP, Instituto de Biociencias, Departamento de Biologia, Av 24A, 1515, CEP 13506 900, Rio Claro, SP, Brazil
    Braz J Biol 65:193-202. 2005
    ..In N. cruentata the fluorochromes DAPI/DA, DAPI/MM and CMA3/DA revealed that the constitutive heterochromatin is rich in AT bases and the NORs possess repetitive sequences of GC bases...
  73. ncbi Conserved chromosome segments in Hylobates hoolock revealed by human and H. leucogenys paint probes
    W Nie
    Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, Peoples Republic of China
    Cytogenet Cell Genet 92:248-53. 2001
    ..hoolock more closely resembles the ancestral karyotype of Hylobates, rather than the karyotype of H. leucogenys...
  74. ncbi A chromosome painting test of the basal eutherian karyotype
    Marta Svartman
    Comparative Molecular Cytogenetics Core, Genetics Branch, National Cancer Institute Frederick, Frederick, MD 21702, USA
    Chromosome Res 12:45-53. 2004
    ..Current attempts lack legitimacy until this situation is corrected...
  75. ncbi ZOO-FISH and R-banding reveal extensive conservation of human chromosome regions in euchromatic regions of river buffalo chromosomes
    L Iannuzzi
    National Research Council CNR, I A B B A M, Naples Italy
    Cytogenet Cell Genet 82:210-4. 1998
    ..Thus, these river buffalo chromosome arms can serve as markers to resolve discrepancies in the nomenclature of cattle and related species...
  76. ncbi Highly conserved chromosomes in an Asian squirrel (Menetes berdmorei, Rodentia: Sciuridae) as demonstrated by ZOO-FISH with human probes
    F Richard
    UMR 147 CNRS, Institut Curie, Section Recherche, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Chromosome Res 11:597-603. 2003
    ..Sciurinae chromosomes appear to be much closer to those of non-rodent mammals than those of Muridae and Cricetidae species studied so far. Thus, they provide an interesting tool to link the rodent genome to those of other mammals...
  77. ncbi A duplicated gene in the breakpoint regions of the 7q11.23 Williams-Beuren syndrome deletion encodes the initiator binding protein TFII-I and BAP-135, a phosphorylation target of BTK
    L A Perez Jurado
    Unidad de Gen etica, Hospital Ni no Jes us, Madred, Spain Stanford University Medical Center
    Hum Mol Genet 7:325-34. 1998
    ..Hemizygosity for GTF2I may also contribute to the WBS phenotype...
  78. pmc Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding
    Viviana Rodriguez
    Department of Cell and Molecular Biology, Genetic Section, Laboratory of Genetics Analysis in Domestic Animals, Faculty of Veterinary, UDELAR, A Lasplaces 1550 CP 11600, Montevideo, Uruguay
    Genet Sel Evol 34:649-56. 2002
    ..The Pearson correlation test showed a positive association between chromosome length and the number of fragile sites (r=0.54). On the contrary, 21 FS were identified on negative R bands while 9 FS were located on positive R bands...
  79. ncbi Phylogenetic inferences of Atelinae (Platyrrhini) based on multi-directional chromosome painting in Brachyteles arachnoides, Ateles paniscus paniscus and Ateles b. marginatus
    E H C de Oliveira
    Institut für Anthropologie und Humangenetik, Department Biologie II, Ludwig Maximilians Universitat, Munich, Germany
    Cytogenet Genome Res 108:183-90. 2005
    ..The chromosomal data support a re-evaluation of the taxonomic status of Ateles b. hybridus...
  80. ncbi Clinical and molecular characterization of individuals with 18p deletion: a genotype-phenotype correlation
    Ulrika Wester
    Unit of Paediatrics, Department of Women and Children s Health, Uppsala University, Sweden
    Am J Med Genet A 140:1164-71. 2006
    ..1 and 18p11.21. The children with a breakpoint at 18p11.1 had all a broad face, which was observed in only one of those with a more distal breakpoint, otherwise no genotype-phenotype correlation of the features was observed...
  81. ncbi Comparative genome analysis in American marsupials: chromosome banding and in-situ hybridization
    M Svartman
    Departamento de Biologia, Instituto de Biociencias, Universidade de Sao Paulo, Sao Paulo SP, Brazil
    Chromosome Res 7:267-75. 1999
    ..In-situ hybridization with total genomic DNA revealed considerable conservation of the euchromatic portions of the three karyotypes and indicated divergence of repetitive DNA sequences in autosomal heterochromatin...
  82. ncbi A case of azoospermia in a bull carrying a Y-autosome reciprocal translocation
    L Iannuzzi
    National Research Council CNR, IABBAM, Laboratory of Animal Cytogenetics and Gene Mapping, Naples, Italy
    Cytogenet Cell Genet 95:225-7. 2001
    ..Analysis on sperm from four different samples revealed azoospermia in the carrier, indicating that the rcp(Y;9) induces sterility in the bull...
  83. ncbi The genome phylogeny of domestic cat, red panda and five mustelid species revealed by comparative chromosome painting and G-banding
    Wenhui Nie
    Key Laboratory of Cellular and Molecular Evolution, The Chinese Academy of Sciences, Kunming, Yunnan, PRC
    Chromosome Res 10:209-22. 2002
    ..Our results support a close phylogenetic relationship between the red panda and mustelids. The homology data presented in these maps will allow us to transfer the cat gene mapping data to other unmapped carnivore species...
  84. ncbi Genome-wide oligonucleotide-based array comparative genome hybridization analysis of non-isolated congenital diaphragmatic hernia
    Daryl A Scott
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Hum Mol Genet 16:424-30. 2007
    ..This method is more sensitive than G-banded chromosome analysis and may find wide application in screening patients with congenital anomalies...
  85. ncbi Molecular cytogenetic characterization of the EBV-producing cell line B95-8 (Saguinus oedipus, Platyrrhini) by chromosome sorting and painting
    S Muller
    Institute of Anthropology and Human Genetics, University of Munich LMU, Germany
    Chromosome Res 9:689-93. 2001
    ..The multicolour probe set described here will be of special value for further evolutionary studies in New World monkeys...
  86. ncbi Evolution of multiple sex chromosomes in the spider genus Malthonica (Araneae: Agelenidae) indicates unique structure of the spider sex chromosome systems
    Jiri Kral
    Laboratory of Arachnid Cytogenetics, Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Vinicna 5, CZ 128 44, Prague 2, Czech Republic
    Chromosome Res 15:863-79. 2007
    ..Structural differentiation of newly formed X chromosomes has been facilitated by heterochromatinization of sex chromosome bivalents observed in prophase I of spider females...
  87. ncbi Phenotypic and cytogenetic spectrum of 9p trisomy
    S A Temtamy
    Clinical Genetics Department, Division of Human Genetics and Genome Research, National Research Centre, Egypt
    Genet Couns 18:29-48. 2007
    ..We also suggest that dosage effects of genes located in 9pter-q22 contribute to the etiology of Dandy-Walker syndrome. We recommend MRI studies as a routine in all cases with trisomy 9p...
  88. ncbi Chromosome banding in Amphibia. XXV. Karyotype evolution and heterochromatin characterization in Australian Mixophyes (Anura, Myobatrachidae)
    M Schmid
    Department of Human Genetics, Biocenter, University of Wurzburg, Wurzburg, Germany
    Cytogenet Genome Res 97:239-53. 2002
    ....
  89. ncbi Patterns of chromosome banding in four nabid species (Heteroptera, Cimicomorpha, Nabidae) with high chromosome number karyotypes
    S Grozeva
    Institute of Zoology, Bulgarian Academy of Sciences, Sofia, Bulgaria
    Hereditas 140:99-104. 2004
    ..The present paper provides new information on the genome organization and new cytological markers useful for a better insight into karyotype evolution of nabid species...
  90. ncbi Visualization of interphase chromosomes in postmitotic cells of the human brain by multicolour banding (MCB)
    I Y Iourov
    National Research Center of Mental Health, Russian Academy of Medical Sciences, Moscow 119152, Russia
    Chromosome Res 14:223-9. 2006
    ..Thus, cytogenetics is no longer limited in its application to dividing cells, which is a great step forward for brain research...
  91. ncbi A molecular cytogenetic analysis of X chromosome repatterning in the Bovidae: transpositions, inversions, and phylogenetic inference
    T J Robinson
    Department of Zoology and Entomology, University of Pretoria, South Africa
    Cytogenet Cell Genet 80:179-84. 1998
    ....
  92. ncbi In situ hybridization and chromosome banding in mammalian species
    R Chaves
    Department of Genetics and Biotechnology, ICETA UTAD, University of Trásos Montes and Alto Douro, Vila Real, Portugal
    Cytogenet Genome Res 96:113-6. 2002
    b>Chromosome banding is often required in conjunction with fluorescent in situ hybridization of labelled probes for chromosome painting, satellite DNA and low-copy sequences to allow identification of chromosomes and simultaneous probe ..
  93. ncbi Boy with seizures (West syndrome) and distal 7q duplication syndrome due to an unbalanced 7q;9p translocation
    B Tuysuz
    Division of Genetics, Department of Pediatrics, Istanbul University Cerrahpasa Faculty of Medicine, Istanbul, Turkey
    Genet Couns 19:29-35. 2008
    ..The boy presented typical facial features of the distal 7q duplication syndrome but no genital anomalies attributable to his distal 9p deletion. We assume that the severe epilepsy is likely due to the trisomy 7q...
  94. ncbi Frequent aberrations of chromosome 8 in aggressive B-cell non-Hodgkin lymphoma
    Barbara Pienkowska-Grela
    Cytogenetic Laboratory, The Memorial M Sklodowska Curie Cancer Centre and Institute, Roentgen Str 5, 02 781 Warsaw, Poland
    Cancer Genet Cytogenet 156:114-21. 2005
    ..This study indicates that the mechanisms of C-MYC activation involved in BLL can be different from that for the BL...
  95. ncbi Subtelomeric deletions of chromosome 6p: molecular and cytogenetic characterization of three new cases with phenotypic overlap with Ritscher-Schinzel (3C) syndrome
    Cheryl Descipio
    Division of Human Genetics and Molecular Biology, The Children s Hospital of Philadelphia, and the University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Am J Med Genet A 134:3-11. 2005
    ..Three forkhead genes (FOXF1 and FOXQ1 from within the critical region, and FOXC1 proximal to this region) were evaluated as potential candidate disease genes for this disorder. No deletions or disease-causing mutations were identified...
  96. ncbi High-resolution molecular cytogenetic analysis of Wilms tumors highlights diagnostic difficulties among small round cell kidney tumors
    Ylva Stewénius
    Department of Clinical Genetics, Lund University Hospital, Lund, Sweden
    Genes Chromosomes Cancer 47:845-52. 2008
    ..The fact that no recurrent translocations were found in the WTs of this study argues against the presence of a frequent pathognomonic translocation in this disease entity...
  97. ncbi Interstitial loss and gain of sequences on chromosome 22 in meningiomas with normal karyotype
    Alexandra Prowald
    Department of Neurosurgery, Neuro Oncology, Saarland University, D 66421 Homburg, Germany
    Int J Oncol 26:385-93. 2005
    ..ii) deletion of the original sequences on chromosome 22, resulting in disomy again (only visible as translocation in metaphase FISH). iii) loss of chromosome 22...
  98. ncbi Molecular cytogenetic analysis of non-small cell lung carcinoma by spectral karyotyping and comparative genomic hybridization
    C Luk
    Departments of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Cancer Genet Cytogenet 125:87-99. 2001
    ..The pattern of overall chromosomal changes detected using combinations of molecular cytogenetic analytical methods suggests that it will be easier to detect recurrent subtype-dependent aberrations in NSCLC...
  99. ncbi A patient with isochromosome 18q, radial-thumb aplasia, thrombocytopenia, and an unbalanced 10;18 chromosome translocation
    Trilochan Sahoo
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Am J Med Genet A 133:93-8. 2005
    ..This additional rearrangement likely has minimal phenotypic consequences, but does raise the possibility that cryptic translocations of telomeric ends of the deleted arm in isochromosome cases may be more common than appreciated...
  100. ncbi Cytogenetic characterization of the TM4 mouse Sertoli cell line. I. Conventional banding techniques, FISH and SKY
    M Guttenbach
    Department of Human Genetics, , , Germany
    Cytogenet Cell Genet 94:71-8. 2001
    ..The results show the usefulness of combining SKY and conventional banding methods for the evaluation of chromosome alterations in widely used cell lines...
  101. ncbi Insights from genomic microarrays into structural chromosome rearrangements
    Jeroen Knijnenburg
    Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center LUMC, Leiden, The Netherlands
    Am J Med Genet A 132:36-40. 2005
    ..We also showed that array results might impact the recurrence risks for relatives of affected individuals. Our data indicate that chromosome rearrangements frequently involve more breaks than current cytogenetic models assume...

Research Grants42

  1. MOLECULAR AND SOMATIC CELL MAPPING OF DEER MOUSE GENES
    DUKE ROGERS; Fiscal Year: 1991
    ..pericentric inversions function as a mechanism to protect gene combinations within these regions? 2) Is chromosome banding similarity among species indicative of underlying genic similarity; and 3) Have portions of the mammalian ..
  2. PHYSICAL MAP OF HUMAN CHROMOSOME 18
    Uta Francke; Fiscal Year: 1993
    ..will be used to determine whether the type of DNA sequences and chromatin structure underlying the chromosome banding patterns can be correlated with the frequency and sites of meiotic crossover events...
  3. Development of Clinically Useful Single Copy FISH Probes
    PETER ROGAN; Fiscal Year: 2005
    ..FISH) is a diagnostic test that distinguishes chromosomal abnormalities not evident by routine chromosome banding. By tagging and hybridizing defined DNA sequences with fluorochromes, abnormalities can be detected for ..
  4. MOLECULAR CYTOGENETIC ANALYSIS OF HUMAN CHROMOSOMES
    JAY GEORGE; Fiscal Year: 1992
    ..been performed by specially trained technologists and professionals with specific expertise in a variety of chromosome banding methods and their interpretation...
  5. OBJECTIVE ANALYSIS OF CHROMOSOME BANDING PATTERNS
    Kenneth Castleman; Fiscal Year: 1999
    ..chromosome analysis instruments, this will allow objective characterization and convenient display of chromosome banding patterns, and quantitative comparison of chromosomes to normal and abnormal prototypes and to each other...
  6. WAVELET ENHANCEMENT OF CHROMOSOME BANDING PATTERNS
    Qiang Wu; Fiscal Year: 2000
    ..aims to develop and commercialize significantly improved software for digital enhancement of the detail of chromosome banding patterns in microscopic images...
  7. E. COLI BASED VECTORS FOR GENE DELIVERY TO HUMAN CELLS
    PETER WARBURTON; Fiscal Year: 2002
    ..These studies propose to develop E. coli-based HAC vectors for human gene therapy that encompass novel approaches for gene delivery, accurate gene expression, and mitotic stability. ..
  8. Chromosome Analysis of Single Cells
    Brynn Levy; Fiscal Year: 2007
    ..abstract_text> ..
  9. MOLECULAR GENETICS OF AUTISM
    Thomas Wassink; Fiscal Year: 2006
    ..The WNT2 findings will be further examined in two-independent patient samples. ..
  10. Biacore T100
    Jeffrey Toretsky; Fiscal Year: 2006
    ..These enhanced capabilities of the T100 will allow projects investigating many types of biologic processes to advance. ..
  11. Nuclear and genomic organization of gene expression
    Jeanne Lawrence; Fiscal Year: 2006
    ..Hypotheses tested have potential to elucidate the functional evolution of the karyotype and such features as cytogenetic bands and conserved gene clusters, which may associate with distinct intranuclear compartments. ..
  12. Lipid Imbalance in CF: Potential CFTR Dysfunction
    Julie Hoover Fong; Fiscal Year: 2006
    ..abstract_text> ..
  13. ANALYSIS OF HUMAN CHROMOSOME 13Q NEOCENTROMERE FORMATION
    PETER WARBURTON; Fiscal Year: 2007
    ..Investigation of human centromere structure, function and formation will greatly facilitate the construction of artificial human chromosomes for use as autonomous gene expression vectors. ..
  14. AN ANIMAL MODEL OF GLUCOCORTICOID RESISTANCE
    Jonathan Scammell; Fiscal Year: 2007
    ..In summary, these studies will provide important insight into the physiological role of FKBP51 while at the same time adding substantial value to the Squirrel Monkey Resource at the University of South Alabama. ..
  15. Analysis of transcription in lens using tiled microarrays (ChIP on chip)
    Ales Cvekl; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  16. Molecular Cytogenetic Tools for the Zebrafish
    Charles Lee; Fiscal Year: 2008
    ..This tool will be used to identify genomic DNA gains and losses which could lead to the identification of novel oncogenes and tumor-suppresor genes that lead to tumorigenesis in many zebrafish mutants. ..
  17. Development of Novel Regimens for the Prevention of Chronic Renal Failure
    CARL SHELLEY; Fiscal Year: 2008
    ..In this way we expect to provide proof-of-principal for new therapeutic regimens that reduce the ability of hypoxia to cause CRF. ..
  18. The repetitive DNA structure of the human genome
    PETER WARBURTON; Fiscal Year: 2009
    ..The studies proposed in this application will facilitate both computational and biological approaches to genomics and provide a unique analysis of a large and relatively neglected portion of our DNA sequence. ..
  19. Akt Inhibitors to Treat Ewing's Sarcoma
    Jeffrey Toretsky; Fiscal Year: 2005
    ..Our findings from this Phase I Sun will hopefully lead to a key compound that can be further developed in a Phase II STTR ultimately leading to clinical trials in patients with ESFT. ..
  20. Imprinted SnoRNA Genes in the PWS deletion Region
    Uta Francke; Fiscal Year: 2005
    ..abstract_text> ..
  21. Conference on Genotype to Phenotype: Focus on Disease
    Uta Francke; Fiscal Year: 2002
    ..techniques, resources and approaches as they arise in different disciplines. In scope and orientation, this conference is unlike any others that have been organized recently. It is timely and its impact will likely be high. ..
  22. PROBING FUNCTIONAL ORGANIZATION WITHIN THE NUCLEUS
    Jeanne Lawrence; Fiscal Year: 2001
    ..It further has potential for diagnosing and elucidating the molecular pathology of splicing defects in OI Type I and other genetic diseases. ..
  23. MOLECULAR MECHANISMS LEADING TO RETT SYNDROME
    Uta Francke; Fiscal Year: 2002
    ..Newborn screening programs could identify presymptomatic mutation carriers. One the causative genetic mechanism is known, understanding of the pathophysiology may lead to early therapeutic intervention. ..
  24. A MULTI-FACETED SEARCH FOR AUTISM DISEASE GENES
    Thomas Wassink; Fiscal Year: 2004
    ..Taken together, the training plan and research proposal provide for a breadth of training, the potential for meaningful discovery, and support for the establishment of an independent research career. ..
  25. Full karyotype single cell interphase analysis
    Santiago Munne; Fiscal Year: 2005
    ..In phase-I we propose to develop two of the three sets of probes, and to test that these probes will work with 90% efficiency after three rounds of hybridization in single polar bodies. ..
  26. CHARACTERIZATION OF A NEW GENE IN P53 MUTANT LFS FAMILY
    ZAKI SHERIF; Fiscal Year: 2004
    ..These studies of LFS should delineate the correlation between genotype and phenotype in LFS and contribute to the general understanding of the processes leading to carcinogenesis. ..
  27. A 13q32 BAC Microarray for chromosome function analysis
    PETER WARBURTON; Fiscal Year: 2004
    ..The development of these types of high-density genomic microarrays will be a major step towards understanding the genomic organization and role of non-protein coding functional DNA elements in our cells. ..
  28. Genetic Determinants of Brain Structure and Disease Risk in Schizophrenia
    Thomas H Wassink; Fiscal Year: 2010
    ....
  29. Function of Genes in Williams Syndrome Deletion Region
    Uta Francke; Fiscal Year: 2005
    ....
  30. Signaling Pathways that Determine Ewings Sarcoma Outcome
    Jeffrey Toretsky; Fiscal Year: 2008
    ..Clearly, if PTPL1 is validated as a supportive oncoprotein and the pathways modulated by PTPL1 are identified, patients with many other tumors that rely on IGF-IR signaling including breast carcinoma could benefit from our findings. ..
  31. E coli-based vectors for BAC delivery to mammalian cells
    PETER WARBURTON; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  32. Canine Brain Tumors - A New Model for Gene Discovery
    Matthew Breen; Fiscal Year: 2006
    ..Characterization of these genetic loci will help to improve the definition of molecular risk and prognosis of human and canine brain tumors, with a potentially major impact on human health. ..
  33. MID ATLANTIC CANCER GENETICS NETWORK
    Constance Griffin; Fiscal Year: 2005
    ....
  34. Array Based CGH for Genome-Wide Analysis of Wilms' Tumor
    Norma Nowak; Fiscal Year: 2004
    ..We will identify the recurrent copy number aberrations present in our WT bank, tile the implicated chromosomal regions on WT subarrays, and identify candidate genes involved in WT and kidney development. ..
  35. Biomarkers for the Progression of Head and Neck Squamous Cell Carcinoma
    Norma Nowak; Fiscal Year: 2009
    ..Clearly, the analysis will also lead to a better understanding of the genetic events leading to invasive HNSCC. ..
  36. Affymetrix 437 Arrayer
    Norma Nowak; Fiscal Year: 2002
    ..The requested instrumentation will afford the DNA Microarray and Genomics Resource with the capacity to continue to provide high quality custom genomic and expression arrays. ..
  37. Mapping and Characterization of Epilespy Genes
    NATALIA LEACH; Fiscal Year: 2006
    ..abstract_text> ..