genetic techniques

Summary

Summary: Chromosomal, biochemical, intracellular, and other methods used in the study of genetics.

Top Publications

  1. ncbi Systematic genetic analysis with ordered arrays of yeast deletion mutants
    A H Tong
    Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6
    Science 294:2364-8. 2001
  2. pmc Statistical significance for genomewide studies
    John D Storey
    Department of Biostatistics, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 100:9440-5. 2003
  3. ncbi Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    S M Elbashir
    Department of Cellular Biochemistry, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Nature 411:494-8. 2001
  4. ncbi A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
  5. ncbi The fluorescent toolbox for assessing protein location and function
    Ben N G Giepmans
    National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA
    Science 312:217-24. 2006
  6. ncbi Genomic control for association studies
    B Devlin
    Department of Psychiatry, University of Pittsburgh, Pennsylvania 15213, USA
    Biometrics 55:997-1004. 1999
  7. pmc Clonal integration of a polyomavirus in human Merkel cell carcinoma
    Huichen Feng
    Molecular Virology Program, University of Pittsburgh Cancer Institute, University of Pittsburgh, 5117 Centre Avenue, Suite 1 8, Pittsburgh, PA 15213, USA
    Science 319:1096-100. 2008
  8. ncbi RNA interference
    Gregory J Hannon
    Cold Spring Harbour Laboratory, New York 11724, USA
    Nature 418:244-51. 2002
  9. ncbi Phylogeny estimation: traditional and Bayesian approaches
    Mark Holder
    Department of Ecology and Evolutionary Biology, 75 North Eagleville Road, University of Connecticut, Storrs, Connecticut 06269 3043, USA
    Nat Rev Genet 4:275-84. 2003
  10. pmc Refinement of tools for targeted gene expression in Drosophila
    Barret D Pfeiffer
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Genetics 186:735-55. 2010

Detail Information

Publications256 found, 100 shown here

  1. ncbi Systematic genetic analysis with ordered arrays of yeast deletion mutants
    A H Tong
    Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6
    Science 294:2364-8. 2001
    ..Systematic application of this approach should produce a global map of gene function...
  2. pmc Statistical significance for genomewide studies
    John D Storey
    Department of Biostatistics, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 100:9440-5. 2003
    ..Our approach avoids a flood of false positive results, while offering a more liberal criterion than what has been used in genome scans for linkage...
  3. ncbi Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    S M Elbashir
    Department of Cellular Biochemistry, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Nature 411:494-8. 2001
    ..Therefore, 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics...
  4. ncbi A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama, 351 0198, Japan
    Nat Biotechnol 20:87-90. 2002
    ..With the improved speed and efficiency of maturation and the increased resistance to environment, Venus will enable fluorescent labelings that were not possible before...
  5. ncbi The fluorescent toolbox for assessing protein location and function
    Ben N G Giepmans
    National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA
    Science 312:217-24. 2006
    ..Small organic fluorescent dyes, nanocrystals ("quantum dots"), autofluorescent proteins, small genetic encoded tags that can be complexed with fluorochromes, and combinations of these probes are highlighted...
  6. ncbi Genomic control for association studies
    B Devlin
    Department of Psychiatry, University of Pittsburgh, Pennsylvania 15213, USA
    Biometrics 55:997-1004. 1999
    ..The performance of our genomic control method is quite good for plausible effects of liability genes, which bodes well for future genetic analyses of complex disorders...
  7. pmc Clonal integration of a polyomavirus in human Merkel cell carcinoma
    Huichen Feng
    Molecular Virology Program, University of Pittsburgh Cancer Institute, University of Pittsburgh, 5117 Centre Avenue, Suite 1 8, Pittsburgh, PA 15213, USA
    Science 319:1096-100. 2008
    ..Thus, MCV may be a contributing factor in the pathogenesis of MCC...
  8. ncbi RNA interference
    Gregory J Hannon
    Cold Spring Harbour Laboratory, New York 11724, USA
    Nature 418:244-51. 2002
    ..RNAi has been cultivated as a means to manipulate gene expression experimentally and to probe gene function on a whole-genome scale...
  9. ncbi Phylogeny estimation: traditional and Bayesian approaches
    Mark Holder
    Department of Ecology and Evolutionary Biology, 75 North Eagleville Road, University of Connecticut, Storrs, Connecticut 06269 3043, USA
    Nat Rev Genet 4:275-84. 2003
    ..The ability of the new approaches to address previously intractable questions is making phylogenetic analysis an essential tool in an increasing number of areas of genetic research...
  10. pmc Refinement of tools for targeted gene expression in Drosophila
    Barret D Pfeiffer
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Genetics 186:735-55. 2010
    ..The increased strength and reliability of these optimized reagents overcome many of the previous limitations of these methods and will facilitate genetic manipulations of greater complexity and sophistication...
  11. ncbi Target site selection by the mariner-like element, Mos1
    Gwénaelle Crénès
    Universite Francois Rabelais de Tours, GICC, Parc de Grandmont, 37200 Tours, France
    Genetica 138:509-17. 2010
    ..Analysis of the physical properties of sequences obtained in vitro and in vivo do not help to explain Mos1 integration preferences, suggesting that other characteristics must be involved in Mos1 target choice...
  12. pmc Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions
    Femke Simmer
    Hubrecht Laboratory, Centre for Biomedical Genetics, Utrecht, The Netherlands
    PLoS Biol 1:E12. 2003
    ..elegans genome. The resulting dataset will be valuable in conjunction with other functional genomics approaches, as well as in other model organisms...
  13. pmc A more efficient RNAi inducible system for tight regulation of gene expression in mammalian cells and xenograft animals
    Jing Zhang
    Immusol Inc, San Diego, CA 92121, USA
    RNA 13:1375-83. 2007
    ..We have demonstrated the high degree of robustness and versatility of this system as applied to several mammalian cells and xenograft animals...
  14. ncbi Chromatin position effects assayed by thousands of reporters integrated in parallel
    Waseem Akhtar
    Division of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cell 154:914-27. 2013
    ..The multiplexed reporter assay is highly flexible in design and can be modified to query a wide range of aspects of gene regulation...
  15. pmc Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis
    Franco J Vizeacoumar
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada
    J Cell Biol 188:69-81. 2010
    ..Although we focused on spindle disassembly in a proof-of-principle study, our integrated HCS-SGA method can be applied to virtually any pathway, making it a powerful means for identifying specific cellular functions...
  16. pmc Comparative analysis of human gut microbiota by barcoded pyrosequencing
    Anders F Andersson
    Swedish Institute for Infectious Disease Control, Solna, Sweden
    PLoS ONE 3:e2836. 2008
    ..Here we applied the technique to analyze microbial communities in throat, stomach and fecal samples. Our results demonstrate the applicability of barcoded pyrosequencing as a high-throughput method for comparative microbial ecology...
  17. ncbi Streptogramin-based gene regulation systems for mammalian cells
    M Fussenegger
    Institute of Biotechnology, Swiss Federal Institute of Technology, ETH Zurich, CH 8093 Zurich, Switzerland
    Nat Biotechnol 18:1203-8. 2000
    ..The streptogramin-based expression technology was functionally compatible with the TetOFF system, thus enabling the selective use of different antibiotics to independently control two different gene activities in the same cell...
  18. ncbi Analysis of gene function in somatic mammalian cells using small interfering RNAs
    Sayda M Elbashir
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Methods 26:199-213. 2002
    ..Because of the robustness of the siRNA knockdown technology, genomewide analysis of human gene function in cultured cells has now become possible...
  19. ncbi Sparking new frontiers: using in vivo electroporation for genetic manipulations
    M Swartz
    Division of Biological Sciences, University of Missouri-Columbia, Columbia, Missouri 65211, USA
    Dev Biol 233:13-21. 2001
    ..In this review, we discuss technical aspects of in vivo electroporation, review recent studies where this approach has been utilized successfully, and identify future directions...
  20. ncbi Palaeomicrobiology: current issues and perspectives
    Michel Drancourt
    Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
    Nat Rev Microbiol 3:23-35. 2005
    ....
  21. pmc The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster
    Edward Ryder
    Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom
    Genetics 167:797-813. 2004
    ..In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence...
  22. pmc Permutation tests for multiple loci affecting a quantitative character
    R W Doerge
    Biometrics Unit, Cornell University, Ithaca, New York 14853, USA
    Genetics 142:285-94. 1996
    ..The search space for minor QTL is unrestricted, and RET-based tests may be more powerful than the CET-based test when the structural model is approximately true...
  23. ncbi Systematic mapping of genetic interaction networks
    Scott J Dixon
    Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 1A7, Canada
    Annu Rev Genet 43:601-25. 2009
    ..Together, our emerging knowledge of the genetic wiring diagrams of eukaryotic and prokaryotic cells is providing a new understanding of the relationship between genotype and phenotype...
  24. pmc Diversifying selection in plant breeding
    Susan McCouch
    Department of Plant Breeding and Genetics at Cornell University, Ithaca, New York, USA
    PLoS Biol 2:e347. 2004
  25. ncbi The art and design of genetic screens: RNA interference
    Michael Boutros
    Division of Signalling and Functional Genomics, German Cancer Research Center and CBTM, University of Heidelberg, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Nat Rev Genet 9:554-66. 2008
    ..Like classical genetic screens in the past, the success of large-scale RNAi surveys depends on a careful development of phenotypic assays and their interpretation in a relevant biological context...
  26. pmc High-dimensional and large-scale phenotyping of yeast mutants
    Yoshikazu Ohya
    Departments of Integrated Biosciences and Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    Proc Natl Acad Sci U S A 102:19015-20. 2005
    ..The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome...
  27. ncbi Highly parallel SNP genotyping
    J B Fan
    llumina, Inc, San Diego, California 92121, USA
    Cold Spring Harb Symp Quant Biol 68:69-78. 2003
  28. pmc Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance
    David J Ecker
    Ibis Division of Isis Pharmaceuticals, 1891 Rutherford Road, Carlsbad, CA 92008, USA
    Proc Natl Acad Sci U S A 102:8012-7. 2005
    ..This analysis method will provide information fundamental to understanding the polymicrobial nature of explosive epidemics of respiratory disease...
  29. pmc Systematic mutagenesis of the Escherichia coli genome
    Yisheng Kang
    Department of Genetics, University of Madison Wisconsin, Rm 206, 445 Henry Mall, Madison, WI 53706, USA
    J Bacteriol 186:4921-30. 2004
    ....
  30. ncbi Synthetic genetic array analysis in Saccharomyces cerevisiae
    Amy Hin Yan Tong
    Methods Mol Biol 313:171-92. 2006
    ..This array-based approach automates yeast genetic analysis in general and can be easily adapted for a number of different screens, including genetic suppression, plasmid shuffling, dosage lethality, or suppression...
  31. ncbi A screen for genes expressed in Drosophila imaginal discs
    Offer Gerlitz
    Institut fur Molekularbiologie, Universitat Zurich, Switzerland
    Int J Dev Biol 46:173-6. 2002
    ..The procedure has been optimized for high-throughput. 2'000 P-element insertions have been established which exhibit expression in imaginal discs...
  32. ncbi Tiling of the Drosophila epidermis by multidendritic sensory neurons
    Wesley B Grueber
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, CA 94143 0725, USA
    Development 129:2867-78. 2002
    ..Together, our data suggest that widespread tiling of the larval body wall involves interactions between growing dendritic processes and as yet unidentified signals that allow avoidance by like dendrites...
  33. ncbi The load of short telomeres, estimated by a new method, Universal STELA, correlates with number of senescent cells
    Laila Bendix
    Danish Aging Research Center, University of Southern Denmark, Odense, Denmark
    Aging Cell 9:383-97. 2010
    ..This new method, Universal STELA, offers some advantages compared to existing methods and can be used to explore many of the unanswered questions in telomere biology including the role that telomeres play in cancer and aging...
  34. ncbi Quantitative detection of DNA methylation states in minute amounts of DNA from body fluids
    Anupam Paliwal
    Epigenetics Group, International Agency for Research on Cancer IARC, 150 cours Albert Thomas, 69008 Lyon, France
    Methods 52:242-7. 2010
    ..Thus, this method should facilitate DNA methylation studies aiming to discover epigenetic biomarkers, and should prove particularly valuable in profiling a large sample series of body fluids from molecular epidemiology studies...
  35. ncbi A novel plasmid curing method using incompatibility of plant pathogenic Ti plasmids in Agrobacterium tumefaciens
    Misugi Uraji
    Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi Hiroshima, Japan
    Genes Genet Syst 77:1-9. 2002
    ..rhizogenes, by replacing the rep gene region of the curing plasmid with that of the corresponding incompatibility...
  36. pmc The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis
    Christopher J Potter
    Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Cell 141:536-48. 2010
    ..The Q system can be expanded to other uses in Drosophila and to any organism conducive to transgenesis...
  37. ncbi Curing of four different plasmids in Yersinia pestis using plasmid incompatibility
    B Ni
    College of Veterinary Medicine, Northwest A and F University, Yangling, Shaanxi, PR China
    Lett Appl Microbiol 47:235-40. 2008
    ..Plasmids are critical for the pathogenicity of Yersinia pestis. In order to carry out a systematic investigation of their role in pathogenesis, we cured plasmids from Y. pestis...
  38. pmc DNA display I. Sequence-encoded routing of DNA populations
    David R Halpin
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
    PLoS Biol 2:E173. 2004
    ..Partitioning steps can be iterated indefinitely, with worst-case yields of 85% per step. These techniques facilitate DNA-programmed chemical synthesis, and thus enable a materials biology that could revolutionize drug discovery...
  39. ncbi A system for stable expression of short interfering RNAs in mammalian cells
    Thijn R Brummelkamp
    Division of Molecular Carcinogenesis, Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, Netherlands
    Science 296:550-3. 2002
    ..Therefore, the pSUPER vector constitutes a new and powerful system to analyze gene function in a variety of mammalian cell types...
  40. ncbi RNA silencing in fungi: mechanisms and applications
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Kobe University, 1 1, Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    FEBS Lett 579:5950-7. 2005
    ..Advantage and disadvantage of RNA silencing as a tool to explore gene function in fungi are discussed...
  41. ncbi A method for parental RNA interference in the wasp Nasonia vitripennis
    Jeremy A Lynch
    Department of Biology, New York University, 100 Washington Square East, New York, New York 10003, USA
    Nat Protoc 1:486-94. 2006
    ..pRNAi experiments in Nasonia can be completed in as little as 2 weeks...
  42. ncbi Conditional control of gene expression in the mouse
    M Lewandoski
    Section of Genetics of Vertebrate Development, Laboratory of Cancer and Developmental Biology, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702 1201, USA
    Nat Rev Genet 2:743-55. 2001
    ..Here, I review these systems and discuss certain studies that exemplify the advantages and limitations of each system...
  43. ncbi Improved solubility of TEV protease by directed evolution
    Susanne van den Berg
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE 171 77 Stockholm, Sweden
    J Biotechnol 121:291-8. 2006
    ..We identified a mutant, TEV(SH), in which three amino acid substitutions result in a five-fold increase in the yield of purified protease with retained activity...
  44. ncbi SNP identification in crop plants
    Martin W Ganal
    TraitGenetics GmbH, Am Schwabeplan 1b, D 06466 Gatersleben, Germany
    Curr Opin Plant Biol 12:211-7. 2009
    ....
  45. ncbi New approaches in GMO detection
    Maddalena Querci
    Molecular Biology and Genomics Unit, Institute for Health and Consumer Protection IHCP, European Commission Joint Research Centre JRC, via Fermi 2749, 21027 Ispra, VA, Italy
    Anal Bioanal Chem 396:1991-2002. 2010
    ..This paper reviews the latest progress made in GMO analysis, taking examples from the most recently developed strategies and tools, and addresses some of the critical aspects related to these approaches...
  46. ncbi Efficiency assessment of the gene trap approach
    A K Voss
    Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Gottingen, Germany
    Dev Dyn 212:171-80. 1998
    ..Evidently, gene trapping does not always provide all three of the features mentioned above. In this paper, we discuss the efficiency of gene trapping and ways in which some problems may be overcome...
  47. pmc Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations
    Devin Dressman
    Howard Hughes Medical Institute and Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins Medical Institutions, 1650 Orleans Street, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 100:8817-22. 2003
    ..BEAMing can be used for the identification and quantification of rare mutations as well as to study variations in gene sequences or transcripts in specific populations or tissues...
  48. pmc Ends-out, or replacement, gene targeting in Drosophila
    Wei J Gong
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Proc Natl Acad Sci U S A 100:2556-61. 2003
    ..When both components are used we find that ends-out targeting can be approximately as efficient as ends-in targeting, and is likely to be generally useful for Drosophila gene targeting...
  49. ncbi Universal linkage system: an improved method for labeling archival DNA for comparative genomic hybridization
    J C Alers
    Department of Pathology, Erasmus University Rotterdam, The Netherlands
    Genes Chromosomes Cancer 25:301-5. 1999
    ..We conclude that ULS is an excellent labeling method for performing CGH on small-fragment-sized DNA...
  50. ncbi DNA Pooling: a tool for large-scale association studies
    Pak Sham
    P080, Institute of Psychiatry, King s College, Denmark Hill, London SE5 8AF, UK
    Nat Rev Genet 3:862-71. 2002
    ..Sophisticated pooling designs are being developed that can take account of hidden population stratification, confounders and inter-loci interactions, and that allow the analysis of haplotypes...
  51. pmc A conditional transposon-based insertional mutagenesis screen for genes associated with mouse hepatocellular carcinoma
    Vincent W Keng
    Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Nat Biotechnol 27:264-74. 2009
    ....
  52. ncbi A genetic approach to visualization of multisynaptic neural pathways using plant lectin transgene
    Y Yoshihara
    Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, Saitama, Japan
    Neuron 22:33-41. 1999
    ..This strategy should greatly facilitate studies on the anatomical and functional organization of the developing and mature nervous system...
  53. pmc Identification of Drosophila MicroRNA targets
    Alexander Stark
    European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 1:E60. 2003
    ..While the screen is likely to miss some targets, our study shows that valid targets can be identified from sequence alone...
  54. ncbi Flow cytometric assessment of TCR-Vbeta expression in the evaluation of peripheral blood involvement by T-cell lymphoproliferative disorders: a comparison with conventional T-cell immunophenotyping and molecular genetic techniques
    William G Morice
    Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    Am J Clin Pathol 121:373-83. 2004
    ..TCR-Vbeta flow cytometric analysis is a powerful clinical laboratory tool that can be used to aid in the rapid diagnosis of peripheral blood involvement by T-cell malignant neoplasms...
  55. ncbi Allelic variation in human gene expression
    Hai Yan
    Sidney Kimmel Comprehensive Cancer Center and Howard Hughes Medical Institute, Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Science 297:1143. 2002
  56. pmc Direct determination of haplotypes from single DNA molecules
    Ming Xiao
    Cardiovascular Research Institute, University of California San Francisco, 513 Parnassus Ave, San Francisco, CA 94143, USA
    Nat Methods 6:199-201. 2009
    ..We demonstrate the utility of this technology by accurately determining the haplotypes consisting of up to 16 single-nucleotide polymorphisms in genomic regions up to 50 kilobases...
  57. pmc UniFrac: a new phylogenetic method for comparing microbial communities
    Catherine Lozupone
    Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA
    Appl Environ Microbiol 71:8228-35. 2005
    ....
  58. ncbi Genetic techniques for the archaea
    Joel A Farkas
    Department of Microbiology and Center for RNA Biology, Ohio State University, Columbus, Ohio 43210
    Annu Rev Genet 47:539-61. 2013
    b>Genetic techniques for the Archaea have undergone a rapid expansion in complexity, resulting in increased exploration of the role of Archaea in the environment and detailed analyses of the molecular physiology and information-processing ..
  59. pmc Unused natural variation can lift yield barriers in plant breeding
    Amit Gur
    The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel
    PLoS Biol 2:e245. 2004
    ..This demonstration of the breaking of agricultural yield barriers provides the rationale for implementing similar strategies for other agricultural organisms that are important for global food security...
  60. pmc Feasibility of genome-scale construction of promoter::reporter gene fusions for expression in Caenorhabditis elegans using a multisite gateway recombination system
    Ian A Hope
    School of Biology, University of Leeds, Leeds, LS2 9JT, UK
    Genome Res 14:2070-5. 2004
    ..The recombinational cloning involved in the Gateway system, which permits the highly efficient and precise transfer of DNA segments between plasmid vectors, makes this technology ideal for genomics research programs...
  61. ncbi The chick; a great model system becomes even greater
    Claudio D Stern
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Dev Cell 8:9-17. 2005
    ..In combination with classical techniques such as grafting and lineage tracing, the chicken is now one of the most versatile experimental systems available...
  62. ncbi dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics
    M M Neff
    Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Plant J 14:387-92. 1998
    ..In addition, a computer program has been developed that facilitates the design of these PCR primers...
  63. pmc Predicting multiallelic genes using unphased and flanking single nucleotide polymorphisms
    Shuying S Li
    Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    Genet Epidemiol 35:85-92. 2011
    ..These preliminary results suggest the feasibility of predicting other polymorphic genetic alleles, since HLA loci are almost certainly among most polymorphic genes...
  64. ncbi RNA interference: a potent tool for gene-specific therapeutics
    Thomas E Ichim
    Department of Surgery, Microbiology and Immunology, University of Western Ontario, ON, Canada
    Am J Transplant 4:1227-36. 2004
    ..In this review we will present a brief history of RNAi, methods of inducing RNAi, application of RNAi in the therapeutic setting, and the possibilities of using this highly promising approach in the context of transplantation...
  65. pmc Reverse genetics in the mosquito Anopheles gambiae: targeted disruption of the Defensin gene
    Stephanie Blandin
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO Rep 3:852-6. 2002
    ..gambiae in vivo. Our results open new perspectives for the study of mosquito gene function in vivo and provide a basis for genome-scale systematic functional screens by targeted gene silencing...
  66. ncbi Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed)
    Brooke J Bevis
    The University of Chicago, Department of Molecular Genetics and Cell Biology, 920 East 58th Street, Chicago, IL 60637, USA
    Nat Biotechnol 20:83-7. 2002
    ..To enhance the solubility of DsRed, we reduced the net charge near the N terminus of the protein. The optimized DsRed variants yield bright fluorescence even in rapidly growing organisms such as yeast...
  67. pmc DNA display III. Solid-phase organic synthesis on unprotected DNA
    David R Halpin
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
    PLoS Biol 2:E175. 2004
    ..Their employment can facilitate the generation of chemically diverse DNA-encoded molecular populations amenable to in vitro evolution and genetic manipulation...
  68. ncbi A gene trap vector system for identifying transcriptionally responsive genes
    E Medico
    Institute for Cancer Research and Treatment, University of Torino School of Medicine, 10060 Candiolo, Italy
    Nat Biotechnol 19:579-82. 2001
    ..The flexibility and efficiency of the GFNR-based trap screening procedure make it suitable for wide surveys of transcriptionally regulated genes...
  69. pmc A DNA vector-based RNAi technology to suppress gene expression in mammalian cells
    Guangchao Sui
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:5515-20. 2002
    ..These findings highlight the general utility of this DNA vector-based RNAi technology in suppressing gene expression in mammalian cells...
  70. pmc RNA affinity tags for purification of RNAs and ribonucleoprotein complexes
    Chatchawan Srisawat
    Department of Biological Chemistry, University of Michigan, 3200 MSRB III, 1150 West Medical Center Drive, Ann Arbor 48109 0606, USA
    Methods 26:156-61. 2002
    ..When properly placed within another RNA molecule, the tags can be used to effect dramatic purification of RNA or ribonucleoprotein complexes from complex mixtures of cellular RNA...
  71. pmc Targeted chromosomal deletions in human cells using zinc finger nucleases
    Hyung Joo Lee
    Department of Chemistry, Seoul National University, Seoul, South Korea
    Genome Res 20:81-9. 2010
    ..Thus, ZFN-induced genomic deletions should be broadly useful as a novel method in biomedical research, biotechnology, and gene therapy...
  72. ncbi Applying genetic techniques to study remote shark fisheries in northeastern Madagascar
    Phaedra Doukakis
    Scripps Institution of Oceanography, Center for Marine Biodiversity and Conservation, University of California, San Diego, La Jolla, CA 92093 1070, USA
    Mitochondrial DNA 22:15-20. 2011
    ..The shark fisheries of Madagascar remain largely unstudied. Remoteness makes fisheries monitoring challenging while the high value of shark fins combined with the extreme poverty in Madagascar creates intensive pressure on shark resources...
  73. ncbi Transforming petals into sepaloid organs in Arabidopsis and oilseed rape: implementation of the hairpin RNA-mediated gene silencing technology in an organ-specific manner
    Marina Byzova
    Bayer BioScience NV, Jozef Plateaustraat 22, Gent 9000, Belgium
    Planta 218:379-87. 2004
    ..napus). These novel flower phenotypes were stable and heritable in both species...
  74. pmc Labeling DNA for single-molecule experiments: methods of labeling internal specific sequences on double-stranded DNA
    Hagar Zohar
    Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA
    Nanoscale 3:3027-39. 2011
    ..Intended for non-specialists seeking accessible solutions to DNA labeling challenges, the approaches outlined emphasize simplicity, robustness, suitability for use by non-biologists, and utility in diverse single-molecule experiments...
  75. pmc DNA display II. Genetic manipulation of combinatorial chemistry libraries for small-molecule evolution
    David R Halpin
    Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
    PLoS Biol 2:E174. 2004
    ....
  76. ncbi Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays
    S V Gordon
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, Cedex 15, France
    Mol Microbiol 32:643-55. 1999
    ..These deletions affect a further seven genes, including a fourth phospholipase, plcD. In summary, the insertions and deletions described here have important implications for our understanding of the evolution of the tubercle complex...
  77. pmc Integrating physical and genetic maps: from genomes to interaction networks
    Andreas Beyer
    Department of Bioengineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nat Rev Genet 8:699-710. 2007
    ..For this purpose, recent studies have sought to classify genetic and physical interactions along several complementary dimensions, such as ordered versus unordered, alleviating versus aggravating, and first versus second degree...
  78. ncbi Meta-analysis of genetic association studies
    Marcus R Munafo
    Cancer Research UK GPRG, Department of Clinical Pharmacology, Radcliffe Infirmary, University of Oxford, Oxford OX2 6HE, UK
    Trends Genet 20:439-44. 2004
    ..If heterogeneity is adequately recognized and taken into account, meta-analysis can confirm the involvement of a genetic variant, but it is not a substitute for an adequately powered primary study...
  79. ncbi Applications of chemical-inducible expression systems in functional genomics and biotechnology
    Jianru Zuo
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing
    Methods Mol Biol 323:329-42. 2006
    ..Protocols are outlined for the inducible overexpression of transgenes, inducible transgene excision, inducible gene silencing, regulated in vivo cDNA generation, inducible activation tagging, and the generation of conditional knockouts...
  80. ncbi A sensitive RNase protection assay to detect transcripts from potentially functional human endogenous L1 retrotransposons
    D M Woodcock
    Sir Donald and Lady Trescowthick Research Laboratories, Peter MacCallum Cancer Institute, Victoria, Australia
    Biochem Biophys Res Commun 222:460-5. 1996
    ..These smaller L1 RNAs do not appear to be derived from transcripts from divergent L1 families but are either discrete shorter transcripts or specifically processed products from longer initial transcripts...
  81. ncbi Common deletion polymorphisms in the human genome
    Steven A McCarroll
    Department of Molecular Biology, Massachusetts General Hospital, 55 Fruit Street, Boston, Massachusetts 02114, USA
    Nat Genet 38:86-92. 2006
    ....
  82. pmc In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae
    Hisao Moriya
    ERATO SORST Kitano Symbiotic Systems Project, Japan Science and Technology Agency, Shibuya ku, Tokyo, Japan
    PLoS Genet 2:e111. 2006
    ..The data were used to evaluate the current computational model, and refinements to the model were suggested...
  83. pmc Transitive functional annotation by shortest-path analysis of gene expression data
    Xianghong Zhou
    Department of Biostatistics, Harvard School of Public Health, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:12783-8. 2002
    ..We assigned functions for 146 yeast genes that are considered as unknown by the Saccharomyces Genome Database and by the Yeast Proteome Database. These genes constitute around 5% of the unknown yeast ORFome...
  84. pmc Single-nucleotide polymorphism identification assays using a thermostable DNA polymerase and delayed extraction MALDI-TOF mass spectrometry
    L A Haff
    PerSeptive Biosystems, Framingham, Massachusetts 01701, USA
    Genome Res 7:378-88. 1997
    ..More than one primer can be simultaneously extended and then mass-analyzed. The mass spectrometric method thus shows promise for high-volume diagnostic or genotyping applications...
  85. pmc Genome-wide associations of gene expression variation in humans
    Barbara E Stranger
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom
    PLoS Genet 1:e78. 2005
    ..In addition, we demonstrate that the HapMap cell lines themselves may serve as a useful resource for quantitative measurements at the cellular level...
  86. pmc Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast
    Adam M Deutschbauer
    Department of Genetics, Stanford University School of Medicine, California 94305, USA
    Genetics 169:1915-25. 2005
    ..Overall, our results suggest that the primary mechanism of haploinsufficiency in yeast is due to insufficient protein production. We discuss the relevance of our findings in yeast to human haploinsufficiency disorders...
  87. ncbi A novel transgenic technique that allows specific marking of the neural crest cell lineage in mice
    Y Yamauchi
    Institute of Molecular Embryology and Genetics, Center for Animal Resources and Development, Kumamoto University School of Medicine, 4 24 1 Kuhonji, Kumamoto, 862 0976, Japan
    Dev Biol 212:191-203. 1999
    ..We believe that P0-Cre transgenic mice will facilitate many interesting experiments, including lineage analysis, purification, and genetic manipulation of the mammalian neural crest cells...
  88. ncbi Detection of regulatory variation in mouse genes
    Christopher R Cowles
    Whitehead Institute and MIT Center for Genome Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Genet 32:432-7. 2002
    ..The results indicate that larger-scale surveys in both mouse and human could identify a substantial number of genes with common regulatory variation...
  89. pmc Variable expression of Cre recombinase transgenes precludes reliable prediction of tissue-specific gene disruption by tail-biopsy genotyping
    Tim J Schulz
    University of Jena, Institute of Nutrition, Jena, Germany
    PLoS ONE 2:e1013. 2007
    ..This indicates that sustained post hoc analyses in regards to efficacy of disruption for every single study group member may be required...
  90. pmc Prospects for fungus identification using CO1 DNA barcodes, with Penicillium as a test case
    Keith A Seifert
    Biodiversity Mycology and Botany, Environmental Sciences, Agriculture and Agri Food Canada, Ottawa, Ontario, Canada K1A 0C6
    Proc Natl Acad Sci U S A 104:3901-6. 2007
    ..The development of a barcoding system for fungi that shares a common gene target with other kingdoms would be a significant advance...
  91. ncbi Adaptation of GAL4 activators for GAL4 enhancer trapping in zebrafish
    Eri Ogura
    Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
    Dev Dyn 238:641-55. 2009
    ..By the combination of these genetic elements, we have successfully developed enhancer trap lines that activate UAS-dependent reporter genes in a tissue-specific fashion that reflects trapped enhancer activities...
  92. ncbi A novel method to calculate the G+C content of genomic DNA sequences
    C T Zhang
    Department of Physics, Tianjin University, China
    J Biomol Struct Dyn 19:333-41. 2001
    ..Especially, the position and size of the large integron island on the smaller chromosome was precisely predicted. This method would be a useful tool for analyzing genomic sequences...
  93. ncbi Fluorescence in situ hybridization: past, present and future
    Jeffrey M Levsky
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Cell Sci 116:2833-8. 2003
    ..In the future, this technique is likely to have significant further impact on live-cell imaging and on medical diagnostics...
  94. pmc A method for accurate detection of genomic microdeletions using real-time quantitative PCR
    Rosanna Weksberg
    Program in Genetics and Genomic Biology, The Research Institute, The Hospital for Sick Children, Toronto, Canada
    BMC Genomics 6:180. 2005
    ....
  95. pmc Comprehensive human genome amplification using multiple displacement amplification
    Frank B Dean
    Molecular Staging, Inc, 300 George Street, Suite 701, New Haven, CT 06511, USA
    Proc Natl Acad Sci U S A 99:5261-6. 2002
    ..MDA-based WGA is a simple and reliable method that could have significant implications for genetic studies, forensics, diagnostics, and long-term sample storage...
  96. ncbi Comprehensive expression atlas of fibroblast growth factors and their receptors generated by a novel robotic in situ hybridization platform
    Murat Burak Yaylaoglu
    Max Planck Institute of Experimental Endocrinology, Hannover, Germany
    Dev Dyn 234:371-86. 2005
    ..genepaint.org), where individual Fgf and Fgfr expression patterns can be interactively viewed at cellular resolution and where sites of expressions can be retrieved using an anatomy-based search...
  97. pmc Functional complementation of human centromere protein A (CENP-A) by Cse4p from Saccharomyces cerevisiae
    Gerhard Wieland
    Department for Molecular Biology, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D 07745 Jena, Germany
    Mol Cell Biol 24:6620-30. 2004
    ..Generally, the newly developed in vivo complementation assay provides a powerful new tool for studying the function and evolutionary conservation of multiprotein complexes from yeast to humans...
  98. ncbi The GAL4 system : a versatile system for the expression of genes
    David A Elliott
    Wellcome Trust Cancer Research UK Gurdon Institute, and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK
    Methods Mol Biol 420:79-95. 2008
    ..Here we describe the GAL4 system highlighting the properties that make it a powerful tool for the analysis of gene function in Drosophila and higher organisms...
  99. ncbi RNAi: ancient mechanism with a promising future
    Stephan Geley
    Institute of Pathophysiology, University of Innsbruck, Fritz Pregl Str 3, A 6020, Austria
    Exp Gerontol 39:985-98. 2004
    ....
  100. ncbi Development of the Drosophila mushroom bodies: sequential generation of three distinct types of neurons from a neuroblast
    T Lee
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Development 126:4065-76. 1999
    ..This study illustrates the cellular organization of mushroom bodies and the development of different MB neurons at the single cell level. It allows for future studies on the molecular mechanisms of mushroom body development...
  101. ncbi A general approach to single-nucleotide polymorphism discovery
    G T Marth
    Washington University Department of Genetics and Genome Sequencing Center, St Louis, Missouri, USA
    Nat Genet 23:452-6. 1999
    ..We demonstrate this approach by accurate SNP predictions in human ESTs aligned to finished and working-draft quality genomic sequences, a data set representative of the typical challenges of sequence-based SNP discovery...

Research Grants74

  1. A unique approach to identify markers for congenital syphilis and neurosyphilis
    Nikhat Parveen; Fiscal Year: 2010
    ..pallidum cannot be grown in vitro. This limits the ability to investigate this organism thoroughly. (ii) Genetic techniques are not feasible for this spirochete. (iii) T...
  2. Analysis of gene regulation of Vibrio cholerae during infection
    Jun Zhu; Fiscal Year: 2013
    ..We will use a mix of biochemical and genetic techniques to define mechanistically how AphB orchestrates the pathogenic life cycle of V...
  3. Genetic Studies of Optic Atrophy
    Taosheng Huang; Fiscal Year: 2012
    ..Taking advantage of the powerful Drosophila genetic techniques, we created eye-specific somatic mutants...
  4. Genetic Study of PP-Fold Polypeptide-Receptor Axis on Metabolic Syndrome Risk
    PEI AN SHIH; Fiscal Year: 2013
    ..Career Development Plan. I am already well-trained in genetic techniques (small-scale gene-sequencing and genotyping), statistics, and genetic epidemiology, and already function at ..
  5. Microbial Diversity in Mechanisms of Disulfide Bond Formation and Reduction
    Dana Boyd; Fiscal Year: 2013
    ..coli and the archaeon Sulfolobus solfataricus. The array of assays and genetic techniques developed in our lab for analyzing disulfide bond formation in the cytoplasm and periplasm will greatly ..
  6. MOUSE MODELS OF INSULIN RESISTANCE
    Domenico Accili; Fiscal Year: 2013
    ..To unravel the complex biology of this protean syndrome, we have endeavored to apply genetic techniques to probe gene function and tissue interactions related to metabolism, and identify tractable targets for ..
  7. The development of probiotic yeast as an inexpensive vaccine delivery platform
    TRACEY JANE LAMB; Fiscal Year: 2013
    ..Using genetic techniques already established for Saccharomyces cerevisiae I will genetically engineer S...
  8. Genetic Analysis of Mosquito Metamorphosis
    James T Nishiura; Fiscal Year: 2012
    ..In pursuit of this goal, molecular-genetic techniques have been applied to mosquito research...
  9. Neurochemistry/physiology of proopiomelanocortin neurons
    MALCOLM JAMES LOW; Fiscal Year: 2013
    ..We will also use genetic techniques and multilabel immunohistochemistry to study the unexpectedly complex dendrites of POMC neurons that receive ..
  10. Molecular and cellular mechanism of Microcephaly
    Arnold Kriegstein; Fiscal Year: 2013
    ..With innovative approaches including high-temporal time-lapse imaging and molecular genetic techniques, the proposed research will provide new insights into the pathogenesis of MCPH and expand our knowledge of ..
  11. GAS Switch from Colonizing Bacterium to Invasive Pathogen
    Victor Nizet; Fiscal Year: 2012
    ..We are studying the ways in which the GAS bacteria shifts from an innocent member of our normal flora to an invasive pathogen, using molecular genetic techniques, assays of immune function, and mouse models of infection.
  12. Nemo-Like Kinase and the Pathogenesis of Spinal and Bulbar Muscular Atrophy
    TIFFANY WERLYNE TODD; Fiscal Year: 2013
    ..the interaction between NLK and AR will be investigated using transcription assays, molecular biology and genetic techniques in cellular and Drosophila models...
  13. Isolation of new phage enzymes to kill B. anthracis
    Vincent A Fischetti; Fiscal Year: 2013
    ..of at least two lysogen phenotypes - biofilm formation and earthworm colonization - through a variety of genetic techniques, transcription studies, and mutant constructions...
  14. Development of a protein-driven gene targeting technology
    Francesca Storici; Fiscal Year: 2010
    ....
  15. Quantitative analysis of the evolving genotype-to-phenotype map
    DANIEL JAROSZ; Fiscal Year: 2012
    ..High-throughput genetic techniques and next-generation sequencing will provide a mechanistic understanding of this phenomenon...
  16. Quantitative analysis of the evolving genotype-to-phenotype map
    DANIEL JAROSZ; Fiscal Year: 2013
    ..High-throughput genetic techniques and next-generation sequencing will provide a mechanistic understanding of this phenomenon...
  17. Glial Cell Regulation of Circadian Behavior
    F Rob Jackson; Fiscal Year: 2013
    ..Our studies will employ Drosophila so as to be able to utilize sophisticated genetic techniques to study neuron-glia interactions in the circadian system...
  18. Neuronal Cell Cycle and Survival
    Nicholas E Baker; Fiscal Year: 2013
    ..These objectives will be achieved by using transgenic fruit flies and molecular genetic techniques to target RNAs and proteins to specific subsets of photoreceptor cells in a conditional manner...
  19. Estrogen receptor activity in zebrafish mating behaviors
    DANIEL AARON GORELICK; Fiscal Year: 2011
    ..b>Genetic techniques can also be used to produce adults that completely lack gonadal tissue...
  20. Regulation of Aromatase Expression in the Corpus Luteum
    CARLOS OSCAR STOCCO; Fiscal Year: 2012
    ..Molecular biology, protein chemistry, and genetic techniques are currently being used in my laboratory or are available to me at the Proteomics and Informatics Services ..
  21. Estrogen and Memory: Genetic Variation and Neuroimaging
    Susan K Conroy; Fiscal Year: 2013
    ..This study will used advanced neuroimaging and genetic techniques to investigate the ways in which alterations in amount of or response to estrogen affect the brain, with ..
  22. NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASES IN NEONATAL HYPOXIA-ISCHEMIA
    Rafael Galindo; Fiscal Year: 2013
    ..order to test this hypothesis, this proposal will utilized various biochemical, immunological, molecular and genetic techniques to assess the role of these proteins in injured developing neurons through the following aims: 1) To ..
  23. The Role of cAMP/PKA Signaling in Neural Circuits Underlying Memory Formation
    Seth M Tomchik; Fiscal Year: 2012
    ..Dr. Davis'group has pioneered genetic techniques to control gene expression spatially and temporally, and has established techniques for in vivo imaging of ..
  24. The Role of cAMP/PKA Signaling in Neural Circuits Underlying Memory Formation
    Seth M Tomchik; Fiscal Year: 2013
    ..Dr. Davis'group has pioneered genetic techniques to control gene expression spatially and temporally, and has established techniques for in vivo imaging of ..
  25. THE ORGANIZATION OF ANIMAL CELL NUCLEI
    Joseph G Gall; Fiscal Year: 2013
    ..We will extend our studies to include the fly Drosophila, where we can apply sophisticated genetic techniques to better understand the function of sisRNA...
  26. Growth regulation by the Fat signaling pathway
    Kenneth D Irvine; Fiscal Year: 2013
    ..These studies will employ a combination of biochemical, cell biological, and genetic techniques in Drosophila...
  27. Investigating the Role of Atg1 in the Regulation of Glutamate Receptors
    Faith Liebl; Fiscal Year: 2009
    ..Using genetic techniques, we determined mutations of the autophagy-specific gene 1 (Atg1) cause a significant reduction in number of ..
  28. Src Family Kinases and Control of Epithelial Cell Paracellular Permeability
    KURT F AMSLER; Fiscal Year: 2012
    ..Experiments will employ genetic techniques to manipulate the activities of these two SFKs and will monitor two components of paracellular permeability, ..
  29. TB and Innate Immune Regulation of Lung Macrophages
    Larry S Schlesinger; Fiscal Year: 2013
    ..We will use human AMs and monocyte-derived macrophages (MDMs), biochemical and genetic techniques, and a new mouse model to accomplish our goals...
  30. Genetics of Brain and Behavioral Modifications in Response to Social Interactions
    Sergey V Nuzhdin; Fiscal Year: 2013
    ..We will combine frontier genomic and molecular-genetic techniques that allow one to determine with high resolution the transcriptome in subsets of cells...
  31. Research Training: Mental Health Behavior Genetics
    John K Hewitt; Fiscal Year: 2013
    ..The application of biometrical, statistical, and quantitative genetic techniques and the development of quantitative trait loci methods allow the mapping and identification of genes that ..
  32. Admixture Mapping of Glaucoma Genes in African Americans
    Michael A Hauser; Fiscal Year: 2012
    ..We are using a new genetic technique to find genes that contribute to glaucoma in this population. Finding such genes could prevent blindness by leading to better treatments for the disease. ..
  33. Leishmaniasis Drug Development Using an Evolutionary Design Strategy
    Roger G Linington; Fiscal Year: 2010
    ..A number of powerful chemical genetic techniques have recently emerged that provide the opportunity for rapid and accurate identification of molecular ..
  34. Renal Endothelin-1: Control of Sodium Excretion and Blood Pressure
    MARIA MARCELA HERRERA; Fiscal Year: 2013
    ..This will provide a solid basis for the trainee to develop a multi-disciplinary program of research using physiological, cellular/molecular, and genetic techniques to achieve her goal of becoming an independent investigator.
  35. Identification of genetic pathways that regulate neuronal circuits in C. elegans
    SALVATORE JAMES CHERRA; Fiscal Year: 2012
    ..conserved in humans, 2) it has a simple, fully mapped nervous system, 3) it is easy to manipulate through genetic techniques, and 4) it has well-conserved homologs to genes mutated in autism spectrum disorders and epilepsy...
  36. The Role of Yeast Mediator in Transcriptional Regulation
    LAWRENCE CHRISTOPHER MYERS; Fiscal Year: 2012
    ..Although ably studied by yeast genetic techniques over the past 10 to 15 years, very little is known about the direct mechanisms used by Mediator to ..
  37. Mechanism of Myosin Chaperone UNC-45: Structural, Functional &Genetic Approaches
    Sanford I Bernstein; Fiscal Year: 2013
    ..We will use flies with a depleted UNC-45 background in conjunction with the powerful genetic techniques of deficiency mapping and microRNA-enabled knockdown to define these partners...
  38. Biofilms and Enterococcus faecalis Biology
    Gary M Dunny; Fiscal Year: 2013
    ..Our research will employing genetic techniques to identify and characterize previously un-studied enterococcal genes that are important in biofilm ..
  39. MIM regulates Shh target gene expression in tumors
    SCOTT ATWOOD; Fiscal Year: 2011
    ..This proposal seeks to utilize a combination of biochemical, cell biological, and genetic techniques to: 1) Determine the mechanism by which MIM regulates membrane dynamics during ciliogenesis and 2) Determine ..
  40. RiboTag: A novel technique to profile cell type specific gene expression and inv
    GEORGE STANLEY MCKNIGHT; Fiscal Year: 2013
    ..abstract_text> ..
  41. Role of Habenula in Tobacco Addiction
    Ramiro Salas; Fiscal Year: 2013
    ..During the K01 award, I plan to become proficient in imaging and genetic techniques, and to collect enough data to write an R01 grant and at least 3 papers...
  42. Regulatory Mechanisms and Biological Functions of non-coding RNA
    ERIC ALCID; Fiscal Year: 2013
    ..The proposal mainly employs molecular genetic techniques to: 1) uncover the mechanism by which ncRNAs negatively regulates mRNA expression and 2) identify genome-..
  43. Research Training - Developmental Behavioral Genetics
    Michael C Stallings; Fiscal Year: 2013
    ..The application of biometrical, statistical, and quantitative genetic techniques and the development of quantitative trait loci methods allow the mapping and identification of genes that ..
  44. Host-Responses to Mycobacterium Infection in Zebrafish
    Lalita Ramakrishnan; Fiscal Year: 2013
    ..In this proposal specifically, we will use a variety of molecular and genetic techniques to probe pathways of cell death and recruitment during granuloma development, and to identify ..
  45. Dynamic regulation of hepatic SIK1 during fasting and feeding
    Rebecca L Berdeaux; Fiscal Year: 2013
    ..We propose to test this hypothesis using a variety of molecular and genetic techniques. We will investigate the dynamics of SIK1 activity in hepatocytes and liver tissue and explore the ..
  46. Genetic Influences Over Nicotine Withdrawal
    Mariella De Biasi; Fiscal Year: 2011
    ..PUBLIC HEALTH RELEVANCE: Our studies will take advantage of genetically engineered mice and novel genetic techniques, and will combine behavioral analysis, molecular biology, and pharmacology to study the influence of an ..
  47. Pharmacogenomics of Parkinson's Disease
    Haydeh Payami; Fiscal Year: 2013
    ..We have planned a novel integrated approach, using Drosophila for its powerful genetic techniques and NeuroGenetics Research Consortium (NGRC) for its large well-characterized patient population...
  48. CELL TO CELL SIGNALING IN MYCOBACTERIA
    MOSES LUTAAKOME JOLOBA; Fiscal Year: 2010
    ..The specific objectives include: (1) To use genetic techniques to study the role of the Rhomboid-like proteins in the physiology of mycobacteria...
  49. Molecular and Genetic Analysis of Murine Steatohepatitis
    Richard M Green; Fiscal Year: 2010
    ..An understanding of the pathophysiologic mechanisms responsible for the development and progression of NASH will allow for the design of rational therapies of this common hepatic disease that affects millions of Americans. ..
  50. Corticostriatal networks and NMDAR mediation of habitual and flexible action
    Jonathan L Brigman; Fiscal Year: 2013
    ..In order for alcohol research to take full advantage of the molecular and genetic techniques that mouse models permit it is essential to first establish the role of these circuits in that species...
  51. Regulation of cAMP - Dependent Protein Kinase Genes
    GEORGE STANLEY MCKNIGHT; Fiscal Year: 2013
    ..Mouse genetic techniques allow us to investigate this problem in a physiological setting and also provide us with novel tools for ..
  52. In Vivo Control and Functional Visualization of Stem Cell-Driven CNS Regeneration
    Jin Hyung Lee; Fiscal Year: 2010
    ..This will be achieved by combining genetic techniques that will introduce modulatory and/or reporting capability to neural cells based on its cell type...
  53. SPATIAL CELL BIOLOGY OF RETINAL CIRCUIT DEVELOPMENT
    Daniel Kerschensteiner; Fiscal Year: 2013
    ..contribution of mitochondria to dendritic and synaptic development and function will be tested using genetic techniques to selectively interfere with mitochondrial localization or sensitize them to laser-ablation...
  54. Function of human regulatory cells in the intestinal mucosa of humanized mice
    JEREMY ALLEN GOETTEL; Fiscal Year: 2013
    ..short-term goals for this fellowship are 1) to become proficient in immunology using biochemical and genetic techniques to investigate mechanistically immune-mediated pathologies;2) to acquire expertise in conceptually and ..
  55. Automatic 3D Quantification of Synapse Distribution in Complex Dendritic Arbor
    Jie Zhou; Fiscal Year: 2013
    ..While advanced multidimensional images, in conjunction with single-cell genetic techniques, have afforded an unprecedented opportunity to understand synapse development at a new level, there is a ..
  56. Circadian Clock Gene Polymorphisms Associated with Depression
    Carl Hirschie Johnson; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: This project will use genetic techniques to study the relationship between daily biological clocks and depression in humans...
  57. Activation Mechanism of Cu/Zn Superoxide Dimutase
    JEFFREY LEITCH; Fiscal Year: 2010
    ..We will use a combination of biochemical and yeast genetic techniques to test the role of the disulfide...
  58. Genetic analysis of innate immunity using C. elegans
    Alejandro Aballay; Fiscal Year: 2013
    ..In this proposal specifically, we will use a variety of molecular and genetic techniques to explore the general hypothesis that the nervous system regulates immune homeostasis during host response ..
  59. Genetic, Behavioral, &Neurobiological Substrates of Nicotine Withdrawal
    Thomas J Gould; Fiscal Year: 2012
    ..This proposal will use traditional behavioral genetic techniques and genetically modified mice to achieve this goal...
  60. Prefrontal Cortex BDNF and Amygdala-Dependent Emotional LearningChallenge Area:
    Kerry J Ressler; Fiscal Year: 2010
    ..Through a combination of anatomical, behavioral, molecular, and genetic techniques, these experiments have the potential to resolve an outstanding issue in the role of Brain Derived ..
  61. OASIS to identify and analyze natural product biosynthesis
    Michael D Burkart; Fiscal Year: 2013
    ..biomedical problems that have yet to be determined and remain currently unstudied due to the limitations of genetic techniques. The application of OASIS offers a new opportunity to evaluate these pathways through combining specific ..
  62. Characterization of the mechanisms underlying rubrospinal control of movement
    NALINI ANNE COLACO; Fiscal Year: 2011
    ..In this project, I will use genetic techniques to specifically and completely label the rubrospinal population, enabling the fine mapping of rubrospinal ..
  63. MOLECULAR ANALYSIS OF CHROMOSOME SEGREGATION IN YEAST
    Clarence Chan; Fiscal Year: 1999
    ..An alternative explanation would be that the sli mutations lead to an overproduction of PP1. He suggests experiments to test all three hypotheses, as well as the cloning and characterization of the wild type sli genes. ..
  64. MECHANISM BASED DRUG SELECTION AND DESIGN FOR T GONDII
    Keith Joiner; Fiscal Year: 1999
    ..funding cycle of this grant, we have used a combination of pharmacological screening, cell biological, and genetic techniques to characterize potential therapeutic targets and identify novel classes of parasiticidal drugs...
  65. GENETIC STUDY OF PROTEASES IN BACTEROIDES GINGIVALIS
    Jeffrey Felton; Fiscal Year: 1991
    ..Molecular genetic techniques will be employed to characterize the genes which encode these factors, as well as the protein products ..
  66. GENETIC ANALYSIS OF PICORNAVIRUS RNA REPLICATION IN VIVO
    JAMES FLANEGAN; Fiscal Year: 2006
    ..In this study, we are using molecular genetic techniques to study poliovirus RNA replication in vitro and in vivo...
  67. PROFILIN I--AN ESSENTIAL PROTEIN IN CELLS AND ANIMALS
    Pascal Goldschmidt Clermont; Fiscal Year: 1999
    ..To characterize profilin's essential function(s), we propose to combine powerful genetic techniques with biochemical experiment on profilin mutants generated by site directed mutagenesis...
  68. Role of Y chromosomal genes in male fertility
    Alexander I Agoulnik; Fiscal Year: 2010
    ..The most frequent cause of etiopathogenetic male infertility revealed by molecular genetic techniques is a deletion of the Y chromosome...
  69. Development of live Francisella tularensis vaccines
    Anders Sjostedt; Fiscal Year: 2009
    ..Given the relative efficacy of these vaccines, together with the availability of recently developed genetic techniques, it should be possible to develop a new generation of F...
  70. HCO3-transporters in Drosophila and Mosquitoes
    MICHAEL ROMERO; Fiscal Year: 2003
    ..To address hypothesis, we propose to two major aims: First, we will use Drosophila genetic techniques and our electrophysiology expertise to determine the physiological phenotypes of NDAE1 mutants at the ..
  71. HUNTINGTON DISEASE IN VENEZUELA AND OTHER STUDIES
    Nancy Wexler; Fiscal Year: 2001
    ..Venezuelan Huntington's disease kindred the gene for HD was localized to chromosome 4p using new molecular genetic techniques; homozygotes for the disease were identified "and the phenomenon of complete dominance" genetically ..
  72. MECHANISM OF INITIATION OF PROTEIN BIOSYNTHESIS
    JOHN HERSHEY; Fiscal Year: 2004
    ..A broad experimental approach is taken that involves biochemical, biophysical, recombinant DNA and genetic techniques to study the structure, function and regulation of specific initiation factors in human and yeast cells...
  73. Identifying Small Molecules To Probe the Role of Heat Shock Factor 1 in Cancer
    Luke Whitesell; Fiscal Year: 2009
    ..therapeutic opportunity has been highlighted by our demonstration that knockdown of HSF1 expression using genetic techniques is well tolerated in normal cells and whole animals but that malignant cells display a profound dependence ..
  74. GENETIC BASIS OF HUMAN CANCER
    Christopher Moskaluk; Fiscal Year: 2000
    ..Tumor suppressor genes are found frequently at sites of genetic less in human cancers. Two molecular genetic techniques have been shown to identify areas of genetic loss in carcinoma: allelotyping of polymorphic markers and ..