parasitic sensitivity tests

Summary

Summary: Tests that demonstrate the relative effectiveness of chemotherapeutic agents against specific parasites.

Top Publications

  1. pmc Spiroindolones, a potent compound class for the treatment of malaria
    Matthias Rottmann
    Swiss Tropical and Public Health Institute, Parasite Chemotherapy, CH 4002 Basel, Switzerland
    Science 329:1175-80. 2010
  2. pmc Chloroquine-resistant Plasmodium vivax, Brazilian Amazon
    Franklin Simoes de Santana Filho
    Emerg Infect Dis 13:1125-6. 2007
  3. pmc Decreasing pfmdr1 copy number in plasmodium falciparum malaria heightens susceptibility to mefloquine, lumefantrine, halofantrine, quinine, and artemisinin
    Amar bir Singh Sidhu
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, Bronx, NY 10461, USA
    J Infect Dis 194:528-35. 2006
  4. pmc Determinants of in vitro drug susceptibility testing of Plasmodium vivax
    B Russell
    International Health Program, Infectious Diseases Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia
    Antimicrob Agents Chemother 52:1040-5. 2008
  5. pmc Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutations
    Amar bir Singh Sidhu
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 298:210-3. 2002
  6. pmc Drug resistance in leishmaniasis
    Simon L Croft
    Drugs for Neglected Diseases Initiative, 1 place Saint Gervais, CH 1201 Geneva, Switzerland
    Clin Microbiol Rev 19:111-26. 2006
  7. pmc In vitro sensitivities of Plasmodium falciparum to different antimalarial drugs in Uganda
    Samuel L Nsobya
    Department of Medicine, Makerere University, Kampala, Uganda
    Antimicrob Agents Chemother 54:1200-6. 2010
  8. pmc Ex vivo susceptibility of Plasmodium falciparum isolates from Dakar, Senegal, to seven standard anti-malarial drugs
    Bécaye Fall
    1Laboratoire d étude de la chimiosensibilité du paludisme, Fédération deslaboratoires, Hôpital Principal de Dakar, Dakar, Senegal
    Malar J 10:310. 2011
  9. ncbi Monitoring of in vitro susceptibilities and molecular markers of resistance of Plasmodium falciparum isolates from Thai-Myanmar border to chloroquine, quinine, mefloquine and artesunate
    Wanna Chaijaroenkul
    Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Rangsit, Patumthani 12121, Thailand
    Acta Trop 113:190-4. 2010
  10. pmc Artemisinin-based combination therapies: a vital tool in efforts to eliminate malaria
    Richard T Eastman
    Department of Microbiology and Immunology, Columbia University College of Physicians and Surgeons, Hammer Health Sciences Center, Room 1502, 701 West 168th Street, New York 10032, New York, USA
    Nat Rev Microbiol 7:864-74. 2009

Research Grants

Detail Information

Publications261 found, 100 shown here

  1. pmc Spiroindolones, a potent compound class for the treatment of malaria
    Matthias Rottmann
    Swiss Tropical and Public Health Institute, Parasite Chemotherapy, CH 4002 Basel, Switzerland
    Science 329:1175-80. 2010
    ..The optimized spiroindolone NITD609 shows pharmacokinetic properties compatible with once-daily oral dosing and has single-dose efficacy in a rodent malaria model...
  2. pmc Chloroquine-resistant Plasmodium vivax, Brazilian Amazon
    Franklin Simoes de Santana Filho
    Emerg Infect Dis 13:1125-6. 2007
  3. pmc Decreasing pfmdr1 copy number in plasmodium falciparum malaria heightens susceptibility to mefloquine, lumefantrine, halofantrine, quinine, and artemisinin
    Amar bir Singh Sidhu
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, Bronx, NY 10461, USA
    J Infect Dis 194:528-35. 2006
    ..These results highlight the importance of pfmdr1 copy number in determining P. falciparum susceptibility to multiple agents currently being used to combat malaria caused by multidrug-resistant parasites...
  4. pmc Determinants of in vitro drug susceptibility testing of Plasmodium vivax
    B Russell
    International Health Program, Infectious Diseases Division, Menzies School of Health Research and Charles Darwin University, Darwin, Australia
    Antimicrob Agents Chemother 52:1040-5. 2008
    ..vivax and suggest that susceptibility to chloroquine may be associated with variable growth rates. These findings have important implications for the phenotypic and downstream genetic characterization of P. vivax...
  5. pmc Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutations
    Amar bir Singh Sidhu
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 298:210-3. 2002
    ..pfcrt mutations increased susceptibility to artemisinin and quinine and minimally affected amodiaquine activity; hence, these antimalarials warrant further investigation as agents to control chloroquine-resistant falciparum malaria...
  6. pmc Drug resistance in leishmaniasis
    Simon L Croft
    Drugs for Neglected Diseases Initiative, 1 place Saint Gervais, CH 1201 Geneva, Switzerland
    Clin Microbiol Rev 19:111-26. 2006
    ....
  7. pmc In vitro sensitivities of Plasmodium falciparum to different antimalarial drugs in Uganda
    Samuel L Nsobya
    Department of Medicine, Makerere University, Kampala, Uganda
    Antimicrob Agents Chemother 54:1200-6. 2010
    ..In summary, we demonstrated in parasites from Kampala a range of sensitivities to older drugs; correlation of sensitivities to CQ, MDAQ, and QN; and good activity against nearly all strains for DHA, LM, and PQ...
  8. pmc Ex vivo susceptibility of Plasmodium falciparum isolates from Dakar, Senegal, to seven standard anti-malarial drugs
    Bécaye Fall
    1Laboratoire d étude de la chimiosensibilité du paludisme, Fédération deslaboratoires, Hôpital Principal de Dakar, Dakar, Senegal
    Malar J 10:310. 2011
    ..To examine whether parasite susceptibility has been affected by the widespread use of ACT, the ex vivo susceptibility of local isolates was assessed at the military hospital of Dakar...
  9. ncbi Monitoring of in vitro susceptibilities and molecular markers of resistance of Plasmodium falciparum isolates from Thai-Myanmar border to chloroquine, quinine, mefloquine and artesunate
    Wanna Chaijaroenkul
    Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Rangsit, Patumthani 12121, Thailand
    Acta Trop 113:190-4. 2010
    ..Moreover, the correlation between pfmdr1 gene amplification and susceptibility of the parasite to MQ, QN and AS was observed (decreased susceptibilities to MQ, QN and AS in isolates with increased pfmdr1 copy number)...
  10. pmc Artemisinin-based combination therapies: a vital tool in efforts to eliminate malaria
    Richard T Eastman
    Department of Microbiology and Immunology, Columbia University College of Physicians and Surgeons, Hammer Health Sciences Center, Room 1502, 701 West 168th Street, New York 10032, New York, USA
    Nat Rev Microbiol 7:864-74. 2009
    ..This Review article discusses our current knowledge about the mode of action of ACTs, their pharmacological properties and the proposed mechanisms of drug resistance...
  11. ncbi Sterol methenyl transferase inhibitors alter the ultrastructure and function of the Leishmania amazonensis mitochondrion leading to potent growth inhibition
    Juliany C F Rodrigues
    Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS Bloco G, Ilha do Fundao, 21949 900 Rio de Janeiro RJ, Brazil
    Protist 158:447-56. 2007
    ..The present study confirms these findings, showing that in Leishmania amazonensis the mitochondrial complex appears to be the first organelle affected after treatment with different SMTI...
  12. pmc Reduced in vitro susceptibility to artemisinin derivatives associated with multi-resistance in a traveller returning from South-East Asia
    Bruno Pradines
    Unité de Recherche en Biologie et Epidémiologie Parasitaires Unité de Recherche pour les Maladies Infectieuses et Tropicales Emergentes UMR 6236, Institut de Medecine Tropicale du Service de Sante des Armees, Marseille, France
    Malar J 10:268. 2011
    ....
  13. pmc Effect of elatol, isolated from red seaweed Laurencia dendroidea, on Leishmania amazonensis
    Adriana Oliveira dos Santos
    Programa de Pós Graduação em Microbiologia, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445, Km 380, CEP 86051 990, Campus Universitario, Londrina, Parana, Brazil
    Mar Drugs 8:2733-43. 2010
    ..Our studies indicated that elatol is a potent antiproliferative agent against promastigote and intracellular amastigote forms, and may have important advantages for the development of new anti-leishamanial chemotherapies...
  14. doi Detection of novel point mutations in the Plasmodium falciparum ATPase6 candidate gene for resistance to artemisinins
    Michela Menegon
    Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita, Rome, Italy
    Parasitol Int 57:233-5. 2008
  15. pmc Geographic structuring of the Plasmodium falciparum sarco(endo)plasmic reticulum Ca2+ ATPase (PfSERCA) gene diversity
    Ronan Jambou
    Institut Pasteur de Dakar, BP 220, Dakar, Senegal
    PLoS ONE 5:e9424. 2010
    ..One possible contributor could be the frequency of haemoglobinopathies that are associated with calcium dysregulation in the erythrocyte...
  16. pmc Assessment of the drug susceptibility of Plasmodium falciparum clinical isolates from africa by using a Plasmodium lactate dehydrogenase immunodetection assay and an inhibitory maximum effect model for precise measurement of the 50-percent inhibitory conc
    Halima Kaddouri
    Centre National de Référence du Paludisme, Laboratoire de Parasitologie, Hôpital Bichat Claude Bernard and Université René Descartes, Paris, France
    Antimicrob Agents Chemother 50:3343-9. 2006
    ..The availability of this simple and highly sensitive pLDH immunodetection assay will provide an easier method for drug susceptibility testing of malaria parasites...
  17. pmc Simple histidine-rich protein 2 double-site sandwich enzyme-linked immunosorbent assay for use in malaria drug sensitivity testing
    Harald Noedl
    Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A 1095 Vienna, Austria
    Antimicrob Agents Chemother 49:3575-7. 2005
    ..The sensitivity is comparable and the drug sensitivity results very closely match those obtained with the commercial ELISA kits (R(2) = 0.979; P < 0.001; mean log difference at the 50% inhibitory concentration = 0.07)...
  18. ncbi Urban malaria in Dakar, Senegal: chemosusceptibility and genetic diversity of Plasmodium falciparum isolates
    Maud Henry
    Unité de Recherche en Biologie et Epidémiologie Parasitaires, Institut de Medecine Tropicale du Service de Sante des Armees, Marseille, France
    Am J Trop Med Hyg 75:146-51. 2006
    ..Areas with urban malaria should use vector control measures and efficient chemoprophylaxis for non-immune populations...
  19. ncbi Malaria drug-sensitivity testing: new assays, new perspectives
    Harald Noedl
    Dept of Specific Prophylaxis and Tropical Medicine, Institute of Pathophysiology, University of Vienna, Kinderspitalgasse 15, A 1095, Vienna, Austria
    Trends Parasitol 19:175-81. 2003
    ..This review examines the various new approaches to the in vitro assessment of malaria drug sensitivity and their limitations...
  20. pmc Comparison of a SYBR green I-based assay with a histidine-rich protein II enzyme-linked immunosorbent assay for in vitro antimalarial drug efficacy testing and application to clinical isolates
    David J Bacon
    Parasitology Program, Naval Medical Research Center Detachment, American Embassy, APO AA 34031, Lima, Peru
    Antimicrob Agents Chemother 51:1172-8. 2007
    ..falciparum with fresh or cultured parasites...
  21. pmc Assessment and continued validation of the malaria SYBR green I-based fluorescence assay for use in malaria drug screening
    Jacob D Johnson
    Principal Investigator, Department of Parasitology, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
    Antimicrob Agents Chemother 51:1926-33. 2007
    ..In conclusion, the MSF assay allows for reliable one-plate high-throughput, automated malaria in vitro susceptibility testing without the expense, time consumption, and hazard of other screening assays...
  22. ncbi Molecular analysis of pfatp6 and pfmdr1 polymorphisms and their association with in vitro sensitivity in Plasmodium falciparum isolates from the Thai-Myanmar border
    Papichaya Phompradit
    Thailand Center of Excellence on Drug Discovery and Development, Thammasat University, Rangsit Campus, Patumthani 12121, Thailand
    Acta Trop 120:130-5. 2011
    ..No association between mutation or amplification of pfatp6 gene and in vitro susceptibility of P. falciparum isolates was found...
  23. pmc Polymorphisms of the pfmdr1 but not the pfnhe-1 gene is associated with in vitro quinine sensitivity in Thai isolates of Plasmodium falciparum
    Teera Poyomtip
    Department of Parasitology, Phramongkutklao College of Medicine, 315 Ratchawithi Rd, Ratchathewi, Bangkok, Thailand
    Malar J 11:7. 2012
    ..falciparum multidrug resistance 1 (pfmdr1), and P. falciparum Na+/H+ exchanger (pfnhe-1). This study was to investigate the role of the pfmdr1 and pfnhe-1 gene on in vitro QN sensitivity in Thai isolates of P. falciparum...
  24. pmc Chloroquine resistance before and after its withdrawal in Kenya
    Leah Mwai
    Kenya Medical Research Institute Wellcome Trust Collaborative Research Programme, Kilifi, Kenya
    Malar J 8:106. 2009
    ..Changes to those that occurred in the dihydrofolate reductase gene (dhfr) that confers resistance to the replacement drug, pyrimethamine/sulphadoxine were also compared...
  25. ncbi Evolution of the pfcrt T76 and pfmdr1 Y86 markers and chloroquine susceptibility 8 years after cessation of chloroquine use in Pikine, Senegal
    Omar Ly
    Laboratoire de Bactério Virologie, Hopital Aristide Le Dantec, 30 Avenue Pasteur, Dakar, Senegal
    Parasitol Res 111:1541-6. 2012
    ..The official discontinuation of CQ use is not completely followed by its total withdrawal from private drug sellers, and the molecule still exerts pressure on local P. falciparum populations...
  26. pmc Bromopyrrole alkaloids as lead compounds against protozoan parasites
    Fernando Scala
    Dipartimento di Chimica delle Sostanze Naturali, Universita di Napoli Federico II, via D Montesano, 49, I 80131, Napoli, Italy
    Mar Drugs 8:2162-74. 2010
    ..Tests against the mammalian L6 cells revealed important clues on therapeutic index of the metabolites. This is the first detailed study on the antiprotozoal potential of marine bromopyrrole alkaloids...
  27. ncbi A novel artemisinin-quinine hybrid with potent antimalarial activity
    John J Walsh
    School of Pharmacy and Pharmaceutical Sciences, Panoz Institute, Trinity College Dublin, Dublin 2, Ireland
    Bioorg Med Chem Lett 17:3599-602. 2007
    ..The activity was superior to that of artemisinin alone, quinine alone, or a 1:1 mixture of artemisinin and quinine...
  28. pmc Polymorphism of Plasmodium falciparum Na(+)/H(+) exchanger is indicative of a low in vitro quinine susceptibility in isolates from Viet Nam
    Veronique Sinou
    UMR MD3 Relations Hôte Parasite, pharmacologie et thérapeutique, Universite de la Mediterranee, Institut de Medecine Tropicale du Service de Sante des Armees, Antenne IRBA Marseille, Marseille, France
    Malar J 10:164. 2011
    ..However, its contribution to QN resistance seems to vary geographically depending on the genetic background of the parasites. Here, the role of this gene was investigated in in vitro QN susceptibility of isolates from Viet Nam...
  29. pmc Improved assessment of plasmodium vivax response to antimalarial drugs by a colorimetric double-site plasmodium lactate dehydrogenase antigen capture enzyme-linked immunosorbent assay
    Pierre Druilhe
    Bio medical Parasitology Unit, Institut Pasteur, 25 rue du Dr Roux, Paris 75015, France
    Antimicrob Agents Chemother 51:2112-6. 2007
    ..falciparum, i.e., in the range of 100 nM for chloroquine and 15 nM for pyronaridine. However, further studies are required to precisely define the cutoff for resistance and the sensitivity to each drug...
  30. pmc Increased tolerance to artemisinin in Plasmodium falciparum is mediated by a quiescence mechanism
    Benoit Witkowski
    CNRS, Laboratoire de Chimie de Coordination, UPR8241, Toulouse, France
    Antimicrob Agents Chemother 54:1872-7. 2010
    ....
  31. ncbi Evidence for different mechanisms of chloroquine resistance in 2 Plasmodium species that cause human malaria
    T Nomura
    Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0425, USA
    J Infect Dis 183:1653-61. 2001
    ..vivax gene. This is evidence that the molecular events underlying P. vivax CQR differ from those in P. falciparum...
  32. pmc Reliability of antimalarial sensitivity tests depends on drug mechanisms of action
    Sharon Wein
    UMR 5235, Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS Universite Montpellier II, Case 107, Place Eugene Bataillon, 34095 Montpellier, Cedex 05, France
    J Clin Microbiol 48:1651-60. 2010
    ..Some of them might not detect the antimalarial potential of new classes of compounds with innovative modes of action, which subsequently could become promising new antimalarial drugs...
  33. pmc A chemical genomic analysis of decoquinate, a Plasmodium falciparum cytochrome b inhibitor
    Tae Gyu Nam
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States
    ACS Chem Biol 6:1214-22. 2011
    ..The basis for this difference was revealed by molecular docking studies, in which both of these inhibitors were shown to have distinctly different modes of binding within the ubiquinol-binding site of cytochrome b...
  34. ncbi The patterns of mutation and amplification of Plasmodium falciparum pfcrt and pfmdr1 genes in Thailand during the year 1988 to 2003
    Mathirut Mungthin
    Department of Parasitology, Phramongkutklao College of Medicine, Ratchathewi, Bangkok, Thailand
    Parasitol Res 107:539-45. 2010
    ..The prominent pattern of pfmdr1 at codons 86/184/1034/1042/1246 was NFSND, with prevalence increasing from 40% to 95% during the 10-year period...
  35. pmc Isothermal microcalorimetry to study drugs against Schistosoma mansoni
    Theresia Manneck
    Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, P O Box, CH 4002 Basel, Switzerland
    J Clin Microbiol 49:1217-25. 2011
    ..IMC is a useful tool for antischistosomal drug discovery that should be further validated. In addition, our data support the use of NTS in in vitro antischistosomal drug assays...
  36. ncbi Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
    Deivid Costa Soares
    Departamento de Imunologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941 590, Brazil
    Parasitol Int 56:135-9. 2007
    ..Importantly, this method preserved the alkaloid properties associated with inhibition of Leishmania growth in macrophages without toxicity to host cells...
  37. pmc Limited ability of Plasmodium falciparum pfcrt, pfmdr1, and pfnhe1 polymorphisms to predict quinine in vitro sensitivity or clinical effectiveness in Uganda
    Frederick N Baliraine
    University of California, San Francisco, CA 94143 0811, USA
    Antimicrob Agents Chemother 55:615-22. 2011
    ..Our data suggest that quinine sensitivity is a complex trait and that known polymorphisms in pfcrt, pfmdr1, and pfnhe1, while associated with quinine sensitivity, are not robust markers for quinine resistance...
  38. ncbi Anti-Trichomonas vaginalis activity of saponins from Quillaja, Passiflora, and Ilex species
    Tábitha Dahmer Rocha
    Laboratório de Fitoquímica e Síntese Orgânica, Departamento de Produção de Matéria Prima, Faculdade de Farmacia, Universidade Federal do Rio Grande do Sul, Av Ipiranga 2752, 90610 000, Porto Alegre, Rio Grande do Sul, Brazil
    Parasitol Res 110:2551-6. 2012
    ..vaginalis activity (MIC = 0.025%). In addition, all samples induced erythrocyte lysis and LDH release. As far as we know, this is the first report demonstrating the potential anti-T. vaginalis activity of these saponins...
  39. pmc SCYX-7158, an orally-active benzoxaborole for the treatment of stage 2 human African trypanosomiasis
    Robert T Jacobs
    SCYNEXIS, Inc, Research Triangle Park, North Carolina, USA
    PLoS Negl Trop Dis 5:e1151. 2011
    ..We have discovered and optimized a novel class of small-molecule boron-containing compounds, benzoxaboroles, to identify SCYX-7158 as an effective, safe and orally active treatment for HAT...
  40. ncbi Anti-infective potential of natural products: how to develop a stronger in vitro 'proof-of-concept'
    Paul Cos
    Laboratory for Microbiology, Parasitology and Hygiene LMPH, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium
    J Ethnopharmacol 106:290-302. 2006
    ....
  41. pmc Chloroquine resistant vivax malaria in a pregnant woman on the western border of Thailand
    Marcus J Rijken
    Shoklo Malaria Research Unit, PO Box 46 Mae Sot, Tak 63110, Thailand
    Malar J 10:113. 2011
    ..vivax. This is the first clinically and laboratory confirmed case of two high-grade CQ resistant vivax parasite strains from Thailand...
  42. pmc Cost-effectiveness of malaria diagnostic methods in sub-Saharan Africa in an era of combination therapy
    Samuel Shillcutt
    Andean Health and Development Saludesa, Quito, Ecuador
    Bull World Health Organ 86:101-10. 2008
    ..To evaluate the relative cost-effectiveness in different sub-Saharan African settings of presumptive treatment, field-standard microscopy and rapid diagnostic tests (RDTs) to diagnose malaria...
  43. pmc Antileishmanial activity of parthenolide, a sesquiterpene lactone isolated from Tanacetum parthenium
    Tatiana Shioji Tiuman
    Departamento de Analises Clinicas, Universidade Estadual de Maringa, Bloco I 90 Sala 123 CCS, Av Colombo 5790, BR 87020 900, Maringa, Parana, Brazil
    Antimicrob Agents Chemother 49:176-82. 2005
    ..These results provide new perspectives on the development of novel drugs with leishmanicidal activities obtained from natural products...
  44. pmc Monitoring of clinical efficacy and in vitro sensitivity of Plasmodium vivax to chloroquine in area along Thai Myanmar border during 2009-2010
    Poonuch Muhamad
    Pharmacology and Toxicology Unit, Graduate Program in Biomedical Sciences, Thammasat University, Thailand
    Malar J 10:44. 2011
    ..vivax in other parts of the world, emphasize the need for closely and continuously monitoring clinical efficacy in conjunction with in vitro sensitivity of P. vivax isolates...
  45. pmc Fexinidazole--a new oral nitroimidazole drug candidate entering clinical development for the treatment of sleeping sickness
    Els Torreele
    Drugs for Neglected Diseases Initiative DNDi, Geneva, Switzerland
    PLoS Negl Trop Dis 4:e923. 2010
    ..To complete the preclinical development and meet the regulatory requirements before initiating human trials, the anti-parasitic properties and the pharmacokinetic, metabolic and toxicological profile of fexinidazole have been assessed...
  46. pmc In vitro sensitivity testing of Leishmania clinical field isolates: preconditioning of promastigotes enhances infectivity for macrophage host cells
    Raquel Inocêncio da Luz
    Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Antwerp University, Antwerp, Belgium
    Antimicrob Agents Chemother 53:5197-203. 2009
    ..In conclusion, the proposed conditioning protocol further contributes toward a more standardized laboratory model for evaluation of the drug sensitivities of field isolates...
  47. pmc Artesunate misuse and Plasmodium falciparum malaria in traveler returning from Africa
    Dea Shahinas
    University of Toronto, Toronto, Ontario, Canada
    Emerg Infect Dis 16:1608-10. 2010
    ..Isolates had an elevated 50% inhibitory concentration to artemisinin, artesunate, and artemether, compared with that of other African isolates. Inappropriate use of artemisinin derivatives can reduce P. falciparum susceptibility...
  48. ncbi Acridinediones: selective and potent inhibitors of the malaria parasite mitochondrial bc1 complex
    Giancarlo A Biagini
    Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK
    Mol Pharmacol 73:1347-55. 2008
    ..falciparum bc(1) Q(o). Dihydroacridinediones represent an entirely new class of bc(1) inhibitors and the potential of these compounds as novel antimalarial drugs is discussed...
  49. pmc In vitro activities of quinine and other antimalarials and pfnhe polymorphisms in Plasmodium isolates from Kenya
    John Okombo
    Kenya Medical Research Institute KEMRI Wellcome Trust Collaborative Research Program, P O Box 230, 80108 Kilifi, Kenya
    Antimicrob Agents Chemother 54:3302-7. 2010
    ..Two DNNND repeats combined with the pfmdr1-86 mutation could be used as an indicator of reduced susceptibility to quinine...
  50. pmc Dynamics of malaria drug resistance patterns in the Amazon basin region following changes in Peruvian national treatment policy for uncomplicated malaria
    David J Bacon
    Parasitology Program, Naval Medical Research Center Detachment, Lima, Peru
    Antimicrob Agents Chemother 53:2042-51. 2009
    ..Importantly, this study demonstrates that the Peruvian triple mutant Pfdhps genotypes are very similar to those found in other parts of South America...
  51. ncbi Naphthoimidazoles promote different death phenotypes in Trypanosoma cruzi
    R F S Menna-Barreto
    Laboratorio de Biologia Celular, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Rio de Janeiro, Brazil
    Parasitology 136:499-510. 2009
    ..However, there are other pathways occurring concomitantly with variable intensities, justifying the need to detail the molecular features involved...
  52. ncbi An in vitro larval motility assay to determine anthelmintic sensitivity for human hookworm and Strongyloides species
    A C Kotze
    Commonwealth Scientific and Industrial Research Organisation Livestock Industries, St Lucia, Queensland 4067, Australia
    Am J Trop Med Hyg 71:608-16. 2004
    ....
  53. ncbi Plasmodium falciparum from Pará state (Brazil) shows satisfactory in vitro response to artemisinin derivatives and absence of the S769N mutation in the SERCA-type PfATPase6
    Isabel D Ferreira
    Centro de Malária e Outras Doenças Tropicais IHMT UEI Biologia Molecular UEI Malária, Lisbon, Portugal
    Trop Med Int Health 13:199-207. 2008
    ....
  54. pmc Plasmodium falciparum drug resistance in Madagascar: facing the spread of unusual pfdhfr and pfmdr-1 haplotypes and the decrease of dihydroartemisinin susceptibility
    Valérie Andriantsoanirina
    Unité de Recherche sur le Paludisme, Institut Pasteur de Madagascar, Antananarivo, Madagascar
    Antimicrob Agents Chemother 53:4588-97. 2009
    ..In the context of the implementation of the new national policy for the fight against malaria, continued surveillance for the detection of P. falciparum resistance in the future is required...
  55. pmc In vitro susceptibilities of Leishmania donovani promastigote and amastigote stages to antileishmanial reference drugs: practical relevance of stage-specific differences
    Marieke Vermeersch
    Laboratory for Microbiology, Parasitology, and Hygiene LMPH, Faculty of Pharmaceutical, Biomedical, and Veterinary Sciences, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
    Antimicrob Agents Chemother 53:3855-9. 2009
    ..Axenic systems can be recommended only for compounds for which no cellular mechanisms are involved, for example, amphotericin B and miltefosine...
  56. ncbi Development of a modified MTT assay for screening antimonial resistant field isolates of Indian visceral leishmaniasis
    Avijit Dutta
    Dept of Immunobiology, Indian Institute of Chemical Biology, 4 Raja S C Mullick Road, Kolkata 700 032, India
    Parasitol Int 54:119-22. 2005
    ..Considering the growing problem of antimonial unresponsiveness in the Indian subcontinent, this modified MTT assay is a useful tool for Leishmania research...
  57. ncbi Plasmodium vivax: isotopic, PicoGreen, and microscopic assays for measuring chloroquine sensitivity in fresh and cryopreserved isolates
    Varakorn Kosaisavee
    Faculty of Public Health, Mahidol University, Bangkok, Thailand
    Exp Parasitol 114:34-9. 2006
    ..Future efforts should focus on further development of high throughput assays such as the PicoGreen assay as described in this study...
  58. ncbi In vitro activity of chloroquine, quinine, mefloquine and halofantrine against Gabonese isolates of Plasmodium falciparum
    Jérôme Mezui Me Ndong
    Centre International de Recherches Medicales de Franceville, Gabon, Central Africa
    Trop Med Int Health 8:25-9. 2003
    ..To determine the in vitro activity of antimalarial drugs against isolates of Plasmodium falciparum in Gabon...
  59. pmc Discovery of potent small-molecule inhibitors of multidrug-resistant Plasmodium falciparum using a novel miniaturized high-throughput luciferase-based assay
    Edinson Lucumi
    Department of Chemical and Biomolecular Engineering and Penn Center for Molecular Discovery, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Antimicrob Agents Chemother 54:3597-604. 2010
    ..Ultimately, this study may provide new probes to understand the molecular details of the malaria life cycle and to identify new antimalarials...
  60. ncbi Activity of novel nicotinic anthelmintics in cut preparations of Caenorhabditis elegans
    Elizabeth Ruiz-Lancheros
    Institute of Parasitology, McGill University, Macdonald Campus, 21111 Lakeshore Road, Ste Anne de Bellevue, Quebec, Canada H9X 3V9
    Int J Parasitol 41:455-61. 2011
    ..The permeability properties of the C. elegans cuticle may be more restrictive than those of adult parasites, calling into question primary anthelmintic screening strategies that rely on this model organism...
  61. pmc Dynamics of pfcrt alleles CVMNK and CVIET in chloroquine-treated Sudanese patients infected with Plasmodium falciparum
    Nahla B Gadalla
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK
    Malar J 9:74. 2010
    ....
  62. pmc Plasmodium falciparum resistance to cytocidal versus cytostatic effects of chloroquine
    Michelle F Paguio
    Department of Chemistry, Georgetown University, Washington, DC 20057, United States
    Mol Biochem Parasitol 178:1-6. 2011
    ..falciparum. The results have important implications for development of new antimalarial drugs and for fully defining the genetic loci that confer clinically relevant antimalarial drug resistance phenomena...
  63. ncbi Synthesis and in vitro and in vivo evaluation of antimalarial polyamines
    Lydia P P Liew
    School of Chemical Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
    Eur J Med Chem 69:22-31. 2013
    ..3-9.5 nM, and selectivity indices (SI) of 42,300 to 4880. In vivo evaluation of three analogues against Plasmodium berghei was undertaken, with one demonstrating a modest 27.9% reduction in parasitaemia...
  64. pmc Phenotypic changes in artemisinin-resistant Plasmodium falciparum lines in vitro: evidence for decreased sensitivity to dormancy and growth inhibition
    Franka Teuscher
    Drug Resistance and Diagnostics, Australian Army Malaria Institute, Brisbane, Australia
    Antimicrob Agents Chemother 56:428-31. 2012
    ..Our results demonstrate that the AL resistance phenotype has (i) decreased sensitivity of mature-stage parasites, (ii) decreased sensitivity of the ring stage to the induction of dormancy, and (iii) a faster recovery from dormancy...
  65. pmc Optimizing the HRP-2 in vitro malaria drug susceptibility assay using a reference clone to improve comparisons of Plasmodium falciparum field isolates
    Wiriya Rutvisuttinunt
    Department of Immunology and Medicine, US Army Medical Corps, Armed Forces Research Institute of Medical Sciences USAMC AFRIMS, Bangkok, Thailand
    Malar J 11:325. 2012
    ..Although in vitro anti-malarial susceptibility tests are widely used, uncertainties remain regarding interpretation of P. falciparum field isolate values...
  66. ncbi Establishment of a panel of reference Trypanosoma evansi and Trypanosoma equiperdum strains for drug screening
    K Gillingwater
    Parasite Chemotherapy, Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, Socinstrasse 57, 4002 Basel, Switzerland
    Vet Parasitol 148:114-21. 2007
    ..This panel of characterised strains with known drug sensitivities and resistances will be of great value for the screening of new active compounds, in comparison with the four standard drugs currently available...
  67. ncbi In vitro and in vivo interaction of synthetic peroxide RBx11160 (OZ277) with piperaquine in Plasmodium models
    Christopher Snyder
    Swiss Tropical Institute, Socinstrasse 57, CH 4002 Basel, Switzerland
    Exp Parasitol 115:296-300. 2007
    ..1) was identified, suggesting that a RBx11160-piperaquine combination therapy in humans should allow each molecule to exert its full antimalarial effect...
  68. ncbi Antileishmanial activity of Eugenol-rich essential oil from Ocimum gratissimum
    Tania Ueda-Nakamura
    Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Estadual de Maringa, Brazil
    Parasitol Int 55:99-105. 2006
    ..The essential oil showed no cytototoxic effects against mammalian cells. This set of results suggests that O. gratissimum essential oil and its compounds could be used as sources for new antileishmanial drugs...
  69. pmc Ex vivo drug sensitivity profiles of Plasmodium falciparum field isolates from Cambodia and Thailand, 2005 to 2010, determined by a histidine-rich protein-2 assay
    Stuart D Tyner
    Department of Immunology and Medicine, US Army Medical Corps, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Malar J 11:198. 2012
    ....
  70. pmc Differential association of Plasmodium falciparum Na+/H+ exchanger polymorphism and quinine responses in field- and culture-adapted isolates of Plasmodium falciparum
    Stéphane Pelleau
    Institut Pasteur de Dakar, BP 220 Dakar, Senegal
    Antimicrob Agents Chemother 55:5834-41. 2011
    ..However, we do not exclude the possibility that in particular settings, Pfnhe-1 polymorphism can be used as a molecular marker for surveillance of quinine resistance...
  71. ncbi A high-throughput assay for the identification of drugs against late-stage Plasmodium falciparum gametocytes
    Christopher L Peatey
    Malaria Biology Laboratory, Queensland Institute of Medical Research, 300 Herston Rd, Herston, Brisbane, Australia
    Mol Biochem Parasitol 180:127-31. 2011
    ..Here we describe the development of a robust and simple assay that is amenable to a high throughput format for the discovery of new antigametocyte drugs...
  72. pmc Flow cytometry for the evaluation of anti-plasmodial activity of drugs on Plasmodium falciparum gametocytes
    Severine Chevalley
    Université de Toulouse 3, LPSNPR Laboratoire pharmacochimie des substances naturelles et pharmacophores redox, Toulouse, France
    Malar J 9:49. 2010
    ..Gametocytes were treated for 48 h with different drug concentrations and the gametocytaemia was then determined by flow cytometry and compared with visual estimation by microscopy...
  73. pmc In vitro activity of ferroquine (SSR 97193) against Plasmodium falciparum isolates from the Thai-Burmese border
    Marion Barends
    Shoklo Malaria Research Unit, Mae Sot, Tak, Thailand
    Malar J 6:81. 2007
    ..The aim of the present study was to investigate the activity of ferroquine against P. falciparum isolates from an area with a known high multi-drug resistance rate...
  74. pmc Use of the atmospheric generators for capnophilic bacteria Genbag-CO2 for the evaluation of in vitro Plasmodium falciparum susceptibility to standard anti-malarial drugs
    Aurélie Pascual
    Unité de Recherche en Biologie et Epidémiologie Parasitaires, Institut de Recherche Biomédicale des Armées Antenne de Marseille, Marseille, France
    Malar J 10:8. 2011
    ..The Genbag® system associated with the atmospheric generators for capnophilic bacteria Genbag CO2® was used for in vitro susceptibility test of nine standard anti-malarial drugs and compared to standard incubator conditions...
  75. ncbi Comparison of novel and existing tools for studying drug sensitivity against the hookworm Ancylostoma ceylanicum in vitro
    Lucienne Tritten
    Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, CH 4002 Basel, Switzerland
    Parasitology 139:348-57. 2012
    ..Among all assays tested the xCELLigence System performed best on adult worms as the test was accurate, simple, required a minimal number of worms and offered the possibility for conducting a medium-throughput screening...
  76. ncbi Antimalarial bromotyrosine derivatives from the Australian marine sponge Hyattella sp
    Xinzhou Yang
    Eskitis Institute, Griffith University, Brisbane, QLD 4111, Australia
    J Nat Prod 73:985-7. 2010
    ..4 and 0.87 microM, respectively, while 1 showed 98% inhibition at 40 microM against the chloroquine-resistant (Dd2) strain of P. falciparum...
  77. pmc In vitro susceptibility to quinine and microsatellite variations of the Plasmodium falciparum Na+/H+ exchanger (Pfnhe-1) gene: the absence of association in clinical isolates from the Republic of Congo
    Sebastien Briolant
    Unite de Parasitologie, Unite Mixte de Recherche 6236, Institut de Recherche Biomédicale des Armées Antenne de Marseille, Marseille, France
    Malar J 10:37. 2011
    ..In the present study, association between Pfnhe and QNR is investigated in a series of isolates from central Africa...
  78. ncbi Dose-response assay templates for in vitro assessment of resistance to benzimidazole and nicotinic acetylcholine receptor agonist drugs in human hookworms
    Andrew C Kotze
    CSIRO Livestock Industries, St Lucia, Brisbane, Australia
    Am J Trop Med Hyg 81:163-70. 2009
    ..These assays will have immediate applicability in monitoring for the emergence of drug resistance in human hookworm populations...
  79. ncbi Evaluation of Turkish seaweeds for antiprotozoal, antimycobacterial and cytotoxic activities
    Sevda Süzgeç-Selçuk
    Department of Pharmacognosy, Faculty of Pharmacy, University of Istanbul, Istanbul, Turkey
    Phytother Res 25:778-83. 2011
    ..This is the second study investigating the antiprotozoal activities of Turkish marine algae and identifies Dasya pedicellata, an understudied algal species, as a candidate for further studies...
  80. pmc Mining a cathepsin inhibitor library for new antiparasitic drug leads
    Kenny K H Ang
    The Small Molecule Discovery Center, University of California San Francisco, San Francisco, California, United States of America
    PLoS Negl Trop Dis 5:e1023. 2011
    ..The end products of this effort include the identification of sub-micromolar cell-active leads as well as the elucidation of structure-activity trends that can guide further optimization efforts...
  81. ncbi Amplification of Plasmodium falciparum multidrug resistance gene 1 in isolates from Gabon
    Anne Catrin Uhlemann
    Department of Cellular and Molecular Medicine, Infectious Diseases Group, St George s Hospital Medical School, London, UK
    J Infect Dis 192:1830-5. 2005
    ..Nevertheless, the detection of multicopy pfmdr1 in African parasites suggests a high potential for rapid selection for resistance, implying that mefloquine use in Africa should be considered only as part of combination therapy...
  82. ncbi Antiretrovirals as antimalarial agents
    Tina S Skinner-Adams
    Malaria Biology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research and School of Population Health, University of Queensland, St Lucia, Queensland, Australia
    J Infect Dis 190:1998-2000. 2004
    ..These findings are particularly important in light of both the high rate of malaria and HIV-1 coinfection in sub-Saharan Africa and the effort to employ highly active antiretroviral therapy in these regions...
  83. pmc A malaria gametocytocidal assay using oxidoreduction indicator, alamarBlue
    Takeshi Q Tanaka
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20892, United States
    Mol Biochem Parasitol 177:160-3. 2011
    ..01). Six anti-malarials were also tested and at 10 μM only primaquine and dihydroartemisinin (DHA) had gametocytocidal activity. This new assay provides an important tool to efficiently screen compounds for gametocytocidal activity...
  84. pmc Identifying apicoplast-targeting antimalarials using high-throughput compatible approaches
    Eric H Ekland
    Department of Microbiology and Immunology, Columbia University College of Physicians and Surgeons, 701 W 168th St, New York, NY 10032, USA
    FASEB J 25:3583-93. 2011
    ..These assays provide the foundation for high-throughput campaigns to identify novel chemotypes for combination therapies to treat multidrug-resistant malaria...
  85. pmc Evidence for a central role for PfCRT in conferring Plasmodium falciparum resistance to diverse antimalarial agents
    David J Johnson
    Molecular and Biochemical Parasitology Group, Liverpool School of Tropical Medicine, Liverpool L3 5QA, United Kingdom
    Mol Cell 15:867-77. 2004
    ..Evidence for the presence of this mutation in a Southeast Asian isolate supports the argument for a broad role for PfCRT in determining levels of susceptibility to structurally diverse antimalarials...
  86. ncbi Potent antimalarial 4-pyridones with improved physico-chemical properties
    José M Bueno
    Tres Cantos Medicines Development Campus, Diseases of the Developing World, GlaxoSmihKline, C Severo Ochoa, 2, 28760 Tres Cantos, Madrid, Spain
    Bioorg Med Chem Lett 21:5214-8. 2011
    ....
  87. ncbi Changes in susceptibility of Plasmodium falciparum to artesunate in vitro in Yunnan Province, China
    Henglin Yang
    Yunnan Institute of Parasitic Diseases, Simao, Yunnan Province 665000, P R China
    Trans R Soc Trop Med Hyg 97:226-8. 2003
    ..The results suggest that P. falciparum is generally susceptible to artemisinin derivatives but indicate a reduction in susceptibility during the study period in areas where the drugs have been used for a long time...
  88. ncbi Amplification of pvmdr1 associated with multidrug-resistant Plasmodium vivax
    R Suwanarusk
    International Health Division, Menzies School of Health Research, Darwin, Australia
    J Infect Dis 198:1558-64. 2008
    ..Multidrug-resistant strains of Plasmodium vivax are emerging in Southeast Asia...
  89. pmc Plasmodium falciparum Na+/H+ exchanger 1 transporter is involved in reduced susceptibility to quinine
    Maud Henry
    Unité de Recherche en Biologie et Epidémiologie Parasitaires, Institut de Medecine Tropicale du Service de Sante des Armees, UMR 6236, Allée du Médecin Colonel Jamot, Parc le Pharo, BP 60109, 13262 Marseille cedex 07, Marseille, France
    Antimicrob Agents Chemother 53:1926-30. 2009
    ..falciparum susceptibility to QN. The validity of the markers should be further supported by analyzing more isolates...
  90. pmc In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
    Autumn S Downey
    Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    J Antimicrob Chemother 64:751-4. 2009
    ..To assess the in vitro activity of the FDA-approved antihelminthic drug pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis...
  91. ncbi In vivo antimalarial activity of essential oils from Cymbopogon citratus and Ocimum gratissimum on mice infected with Plasmodium berghei
    F Tchoumbougnang
    Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
    Planta Med 71:20-3. 2005
    ..1 %, 81.7 % and 86.6 %. The corresponding values for the oil of O. gratissimum at the same concentrations were 55.0 %, 75.2 % and 77.8 %, respectively. Chloroquine (10 mg/kg of mouse, positive control) had a suppressive activity of 100 %...
  92. ncbi Clinical-parasitological response and in-vitro sensitivity of Plasmodium vivax to chloroquine and quinine on the western border of Thailand
    Oumaporn Tasanor
    Pharmacology and Toxicology Unit, Faculty of Allied Health Sciences, Thammasat University Rangsit Campus, Paholyothin Road, Pathumthani 12121, Thailand
    Trans R Soc Trop Med Hyg 100:410-8. 2006
    ....
  93. pmc Quinine-resistant malaria in traveler returning from Senegal, 2007
    Bruno Pradines
    Institut de Medecine Tropicale du Service de Sante des Armees, Marseille, France
    Emerg Infect Dis 16:546-8. 2010
    ..Clinical quinine failure was associated with a 50% inhibitory concentration of 829 nmol/L. Increased vigilance is required during treatment follow-up...
  94. ncbi Molecular detection of benzimidazole resistance in Haemonchus contortus using real-time PCR and pyrosequencing
    G von Samson-Himmelstjerna
    Institute for Parasitology, University of Veterinary Medicine Hannover, Bunteweg 17, 30559 Hannover, Germany
    Parasitology 136:349-58. 2009
    ..Thus, they provide a realistic option for routine molecular resistance testing on farms...
  95. ncbi High-level chloroquine resistance in Sudanese isolates of Plasmodium falciparum is associated with mutations in the chloroquine resistance transporter gene pfcrt and the multidrug resistance Gene pfmdr1
    H A Babiker
    Institute of Cell, Animal, and Population Biology, University of Edinburgh, Edinburgh, EH9 3JT, Scotland, United Kingdom
    J Infect Dis 183:1535-8. 2001
    ..A significant association between pfmdr1-Y86 and pfcrt-T76 was apparent among resistant isolates, which suggests a joint action of the 2 genes in high-level chloroquine resistance...
  96. ncbi In vitro activity of artemisone compared with artesunate against Plasmodium falciparum
    Michael Ramharter
    Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon
    Am J Trop Med Hyg 75:637-9. 2006
    ..14 nmol/L, EC90 = 2.55 nmol/L) than artesunate against fresh Plasmodium falciparum isolates from Gabon and a high-activity correlation indicates a shared drug target...
  97. ncbi Turkish freshwater and marine macrophyte extracts show in vitro antiprotozoal activity and inhibit FabI, a key enzyme of Plasmodium falciparum fatty acid biosynthesis
    I Orhan
    Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, TR 06330 Ankara, Turkey
    Phytomedicine 13:388-93. 2006
    ..falciparum FabI inhibitors from aquatic and marine macrophytes...
  98. pmc A comparative study of a flow-cytometry-based assessment of in vitro Plasmodium falciparum drug sensitivity
    Stephan Karl
    School of Physics, M013, The University of Western Australia, 35 Stirling Highway, Crawley WA 6009, Australia
    Malar J 8:294. 2009
    ..falciparum lactate dehydrogenase assay, the tritiated hypoxanthine incorporation assay, a previously described Sybr Green based plate reader assay and light microscopy...
  99. ncbi In vitro assays for evaluation of drug activity against Leishmania spp
    Luciana Fumarola
    Department of Internal Medicine, Immunology and Infectious Diseases, Microbiology and Immunology Section, University of Bari, Medical School, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy
    Res Microbiol 155:224-30. 2004
    ..Reliable and simple in vitro models are required for large-scale initial screenings. In this review different methods for in vitro evaluation of drug activity against Leishmania spp. are summarized...
  100. pmc Assessment of malaria in vitro drug combination screening and mixed-strain infections using the malaria Sybr green I-based fluorescence assay
    Edgie Mark A Co
    Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
    Antimicrob Agents Chemother 53:2557-63. 2009
    ..In conclusion, the MSF assay allows for reliable antimalarial drug combination screening and provides an important method to discern between homogenous and heterogeneous parasite populations...
  101. ncbi Recovery of chloroquine sensitivity and low prevalence of the Plasmodium falciparum chloroquine resistance transporter gene mutation K76T following the discontinuance of chloroquine use in Malawi
    Toshihiro Mita
    Department of International Affairs and Tropical Medicine, Tokyo Women s Medical University School of Medicine, Tokyo, Japan
    Am J Trop Med Hyg 68:413-5. 2003
    ..29). Withdrawal of the use of chloroquine appears to have resulted in the recovery of chloroquine efficacy and a reduction in the prevalence of K76T. However, other polymorphisms are also expected to contribute to resistance...

Research Grants26

  1. TS PROPHYLAXIS AND DRUG-RESISTANT MALARIA IN MALAWI
    CHRISTOPHER PLOWE; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  2. Pre-Clinical Development of Natural Product Analogues as Antimalarial Agents
    Shuren Zhu; Fiscal Year: 2007
    ..Investigational new drug (IND) application will then be filed with FDA. Radix Pharmaceuticals has strategic alliance with commercial partners for Phase III development. [unreadable] [unreadable] [unreadable]..
  3. Development of Natural Product as Antimalarial Agent
    Shuren Zhu; Fiscal Year: 2007
    ..Investigational new drug (IND) application will then be filed with FDA. [unreadable] [unreadable] [unreadable]..
  4. Randomized, controlled trial of daily trimethoprim-sulfamethoxazole or weekly chl
    CHRISTOPHER PLOWE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  5. TS PROPHYLAXIS AND DRUG-RESISTANT MALARIA IN MALAWI
    CHRISTOPHER PLOWE; Fiscal Year: 2003
    ....
  6. DRUG-RESISTANT MALARIA IN MALAWI
    CHRISTOPHER PLOWE; Fiscal Year: 2002
    ..abstract_text> ..
  7. Plasmodium Falciparum Metal Metabolism
    David Sullivan; Fiscal Year: 2009
    ..Plasmodium parasitism provides a comparison of "simple" erythrocyte cell to more complex infected cell. ..
  8. TOTAL SYNTHESIS OF AZADIRACHTIN
    K Nicolaou; Fiscal Year: 2008
    ..The proposed work is expected to impact the general areas of pharmaceutical and agricultural research, and infectious diseases in particular, through discoveries in synthetic organic chemistry and chemical biology. ..
  9. TOTAL SYNTHESIS OF NATURAL PRODUCTS
    K Nicolaou; Fiscal Year: 2002
    ..3] sigmatropic rearrangement and a radical based ring closure to form the bicyclic skeleton. ..
  10. Host Polymorphisms and Uncomplicated Malaria
    Sunil Parikh; Fiscal Year: 2008
    ..Parikh will be able to utilize modern molecular epidemiology techniques to investigate human genetic factors underlying susceptibility to malaria and he will be well equipped for a career in academic research. ..
  11. Role of iNOS and CAT-2 in defense against CP infection
    Jody Gookin; Fiscal Year: 2006
    ..parvum infection. The PI is presently in the 3rd year of a 5-year K08 from NIDDK. These studies are anticipated to generate key preliminary data for the PI's first R01 application ..
  12. SYNTHESIS OF ANTICANCER AGENTS
    K C Nicolaou; Fiscal Year: 2010
    ..abstract_text> ..
  13. TOTAL SYNTHESIS OF APOPTOLIDIN
    K Nicolaou; Fiscal Year: 2004
    ..The proposed work is expected to have significant impact in the area of cancer chemotherapy and should provide enabling technologies and tools for biology and medicine. ..
  14. Enabling Technologies for Combinatorial Chemistry
    K Nicolaou; Fiscal Year: 2005
    ..abstract_text> ..
  15. Novel Tetracycyclines as Antimalarial Drugs
    Michael Draper; Fiscal Year: 2005
    ..We anticipate that at the end of the Phase II period we will have identified several candidate molecules that are suitable for pre-clinical development. ..
  16. Sterol Biosynthesis in Trypanosomatid Parasites
    Frederick Buckner; Fiscal Year: 2004
    ..The drugs discovered in this research program will hopefully provide better future treatment for patients with these devastating diseases. ..
  17. IMMUNODOMINANT TARGETS OF CMI TO CRYPTOSPORIDIUM
    Jan Mead; Fiscal Year: 2009
    ..This will be accomplished by immunizing mice with a DNA construct of these antigens, assessing their ability to elicit immune responses, and challenging mice with C. parvum to determine the degree of protection achieved. ..
  18. THE PHYSIOLOGY OF DRUG RESISTANT MALARIA
    PAUL ROEPE; Fiscal Year: 2009
    ..Knowledge gained during this research will significantly expand our understanding of malarial parasite physiology and antimalarial drug resistance. ..
  19. Chemoprevention/Therapy Using Non-Calcemic Vitamin D
    Gary Posner; Fiscal Year: 2006
    ..Collectively, these studies will provide important structure- activity insights into the cancer chemoprotective and chemotherapeutic actions and molecular biology of vitamin D3 analogs. ..
  20. SURFACE ANTIGENS OF TREPONEMA PALLIDUM
    Wesley Van Voorhis; Fiscal Year: 2008
    ..Finally, these studies will help define the protective immune response that results after immunization with Tp92 as well as the immune response to Tp92 that occurs during infection. [unreadable] [unreadable]..
  21. Molecular epidemiology of drug resistant malaria
    MIRIAM LAUFER; Fiscal Year: 2008
    ..abstract_text> ..
  22. NO& Epithelial Repair in Cryptosporidiosis
    Jody Gookin; Fiscal Year: 2006
    ..abstract_text> ..
  23. A randomized, controlled clinical trial of chloroquine as chemoprophylaxis versus
    MIRIAM LAUFER; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  24. Inhibition of Alzheimer's Beta-Amyloid Fibril Formation
    Duy Hua; Fiscal Year: 2009
    ..However, soluble oligomeric Abeta showed high neuronal toxicity. Inhibition of the formation of these toxic soluble Abeta oligomers would provide therapeutics for AD. ..
  25. TRIOXANES FOR CHEMOTHERAPY OF MALARIA AND CANCER
    Gary Posner; Fiscal Year: 2005
    ....
  26. Topoisomerases: Target for Antitrypanosomal Therapy
    Theresa Shapiro; Fiscal Year: 2006
    ..Compounds that appear most promising will be evaluated in mice. These studies take a multi-faceted, rational, and tangible approach to the development of much-needed new anti-trypanosomal chemotherapy. ..