serial passage

Summary

Summary: Inoculation of a series of animals or in vitro tissue with an infectious bacterium or virus, as in VIRULENCE studies and the development of vaccines.

Top Publications

  1. ncbi Complete replication of hepatitis C virus in cell culture
    Brett D Lindenbach
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:623-6. 2005
  2. pmc Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines
    Conor J McMeniman
    School of Integrative Biology, The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
    Appl Environ Microbiol 74:6963-9. 2008
  3. pmc Response of hepatitis C virus to long-term passage in the presence of alpha interferon: multiple mutations and a common phenotype
    Celia Perales
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, Madrid, Spain
    J Virol 87:7593-607. 2013
  4. ncbi Molecular analysis of dengue virus attenuation after serial passage in primary dog kidney cells
    B Puri
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889 5607, USA
    J Gen Virol 78:2287-91. 1997
  5. pmc Genetic diversity and purifying selection in West Nile virus populations are maintained during host switching
    Greta V S Jerzak
    Wadsworth Center, New York State Department of Health, 5668 State Farm Rd Slingerlands, NY 12159, USA
    Virology 374:256-60. 2008
  6. ncbi Biologic properties of dengue viruses following serial passage in primary dog kidney cells: studies at the University of Hawaii
    Scott B Halstead
    Department of Tropical Medicine and Medical Microbiology, John A Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA
    Am J Trop Med Hyg 69:5-11. 2003
  7. pmc Hemagglutinin receptor binding avidity drives influenza A virus antigenic drift
    Scott E Hensley
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Science 326:734-6. 2009
  8. ncbi In vitro selection of human cytomegalovirus variants unable to transfer virus and virus products from infected cells to polymorphonuclear leukocytes and to grow in endothelial cells
    M Grazia Revello
    Servizio di Virologia, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, 27100 Pavia, Italy
    J Gen Virol 82:1429-38. 2001
  9. ncbi Modification of dengue virus strains by passage in primary dog kidney cells: preparation of candidate vaccines and immunization of monkeys
    Kenneth H Eckels
    Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 69:12-6. 2003
  10. ncbi Evidence for quasispecies distributions in the human hepatitis A virus genome
    Gloria Sanchez
    Grup Virus Entèrics, Department of Microbiology, University of Barcelona, 08028 Barcelona, Spain
    Virology 315:34-42. 2003

Detail Information

Publications213 found, 100 shown here

  1. ncbi Complete replication of hepatitis C virus in cell culture
    Brett D Lindenbach
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:623-6. 2005
    ..HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals...
  2. pmc Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines
    Conor J McMeniman
    School of Integrative Biology, The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
    Appl Environ Microbiol 74:6963-9. 2008
    ..After long-term serial passage in this cell line, we transferred the mosquito-adapted wMelPop into cell lines derived from two other mosquito ..
  3. pmc Response of hepatitis C virus to long-term passage in the presence of alpha interferon: multiple mutations and a common phenotype
    Celia Perales
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, Madrid, Spain
    J Virol 87:7593-607. 2013
    ..The results suggest that multiple, independent mutational pathways can confer IFN-α resistance to HCV and might explain why no unified picture has been obtained regarding IFN-α resistance in vivo...
  4. ncbi Molecular analysis of dengue virus attenuation after serial passage in primary dog kidney cells
    B Puri
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889 5607, USA
    J Gen Virol 78:2287-91. 1997
    ..parent strain dengue-1 virus West Pac 74 and virulent strain 45AZ5 PDK-O, which was derived from the parent by serial passage in diploid foetal rhesus lung (FRhL-2) and mutagenized with 5-azacytidine...
  5. pmc Genetic diversity and purifying selection in West Nile virus populations are maintained during host switching
    Greta V S Jerzak
    Wadsworth Center, New York State Department of Health, 5668 State Farm Rd Slingerlands, NY 12159, USA
    Virology 374:256-60. 2008
    ..Thus, the abundant genetic variation contributed to WNV populations through infection of mosquitoes and the strong purifying selection contributed by infection of birds may be maintained despite frequent host switching...
  6. ncbi Biologic properties of dengue viruses following serial passage in primary dog kidney cells: studies at the University of Hawaii
    Scott B Halstead
    Department of Tropical Medicine and Medical Microbiology, John A Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA
    Am J Trop Med Hyg 69:5-11. 2003
    b>Serial passage at low dilution of seven different wild-type dengue (DEN) viruses into primary dog kidney (PDK) cell cultures placed selective pressure that resulted in the following changes from parental phenotype: smaller plaques in LLC-..
  7. pmc Hemagglutinin receptor binding avidity drives influenza A virus antigenic drift
    Scott E Hensley
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Science 326:734-6. 2009
    ....
  8. ncbi In vitro selection of human cytomegalovirus variants unable to transfer virus and virus products from infected cells to polymorphonuclear leukocytes and to grow in endothelial cells
    M Grazia Revello
    Servizio di Virologia, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, 27100 Pavia, Italy
    J Gen Virol 82:1429-38. 2001
    ..These findings link EC and PMNL tropism and suggest that PMNL tropism is a late HCMV function...
  9. ncbi Modification of dengue virus strains by passage in primary dog kidney cells: preparation of candidate vaccines and immunization of monkeys
    Kenneth H Eckels
    Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA
    Am J Trop Med Hyg 69:12-6. 2003
    ..A DENV-3 strain lost all monkey infectivity after PDK cell passage. Twelve vaccine candidates were selected for Phase 1 human trials through this selection process...
  10. ncbi Evidence for quasispecies distributions in the human hepatitis A virus genome
    Gloria Sanchez
    Grup Virus Entèrics, Department of Microbiology, University of Barcelona, 08028 Barcelona, Spain
    Virology 315:34-42. 2003
    ..The results indicate that despite antigenic conservation, HAV replicates as a complex distribution of mutants, a feature of viral quasispecies...
  11. ncbi A mouse muscle-adapted enterovirus 71 strain with increased virulence in mice
    Wei Wang
    Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, No 5 Panjiayuan Nanli, Chaoyang Dist, Beijing 100021, PR China
    Microbes Infect 13:862-70. 2011
    ..Further analysis revealed many mutations in different regions of the genome of mouse muscle-adapted virus. Collectively, these data demonstrated the mouse muscle-adapted EV71 0805a with increased virulence in mice...
  12. ncbi Mapping of amino acid residues responsible for adhesion of cell culture-adapted foot-and-mouth disease SAT type viruses
    Francois F Maree
    Transboundary Animal Diseases Programme, Onderstepoort Veterinary Institute, Agricultural Research Council, Onderstepoort 0110, South Africa
    Virus Res 153:82-91. 2010
    ..Therefore, recombinant SAT viruses engineered to express substitutions that induce GAG-binding could be exploited in the rational design of vaccine seed stocks with improved growth properties in cell cultures...
  13. ncbi Measuring HCV infectivity produced in cell culture and in vivo
    Brett D Lindenbach
    Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT, USA
    Methods Mol Biol 510:329-36. 2009
    ..This chapter describes methods for measuring the infectivity of HCV produced in cell culture or recovered from animals experimentally infected with the cell culture-produced virus...
  14. pmc Deletions of neuraminidase and resistance to oseltamivir may be a consequence of restricted receptor specificity in recent H3N2 influenza viruses
    Shelly Gulati
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Virol J 6:22. 2009
    ..Known mechanisms of resistance to NA inhibitors include mutations in the inhibitor binding site, or mutations in the hemagglutinin that reduce avidity for sialic acid and therefore reduce the requirement for NA activity...
  15. ncbi Rapid and spontaneous loss of phthiocerol dimycocerosate (PDIM) from Mycobacterium tuberculosis grown in vitro: implications for virulence studies
    Pilar Domenech
    Research Institute of the McGill University Health Centre, 1625 Pine Ave, West Montreal, QC H3G 1A4, Canada
    Microbiology 155:3532-43. 2009
    ..It is essential that researchers undertaking in vivo virulence studies confirm the presence of PDIM within all recombinant clones and the parental strains they are derived from...
  16. ncbi Experimental evolution of parasites
    D Ebert
    Universitat Basel, Zoologisches Institut, Rheinsprung 9, 4051 Basel, Switzerland
    Science 282:1432-5. 1998
    b>Serial passage experiments are a form of experimental evolution that is frequently used in applied sciences; for example, in vaccine development...
  17. ncbi Serial passage of sheep scrapie inoculum in Suffolk sheep
    A N Hamir
    National Animal Disease Center, ARS, USDA, Ames, IA 50010, USA
    Vet Pathol 46:39-44. 2009
    ....
  18. pmc Mosquitoes put the brake on arbovirus evolution: experimental evolution reveals slower mutation accumulation in mosquito than vertebrate cells
    Nikos Vasilakis
    Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America
    PLoS Pathog 5:e1000467. 2009
    ..Moreover, these findings suggest that patterns of genetic evolution may differ between viruses replicating in mammalian and mosquito cells...
  19. pmc Arbovirus evolution in vivo is constrained by host alternation
    Lark L Coffey
    Department of Pathology and Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA
    Proc Natl Acad Sci U S A 105:6970-5. 2008
    ..These results suggest that arbovirus adaptation and evolution is limited by obligate host alternation and predict that arboviral emergence via host range changes may be less frequent than that of single host animal RNA viruses...
  20. ncbi Serial passage of foot-and-mouth disease virus in sheep reveals declining levels of viraemia over time
    Gareth J Hughes
    Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
    J Gen Virol 83:1907-14. 2002
    ..Here, the results are reported of a novel experiment designed specifically to assess the effects of serial passage of foot-and-mouth disease virus (FMDV) in experimental groups of sheep...
  21. pmc Production of baculovirus defective interfering particles during serial passage is delayed by removing transposon target sites in fp25k
    Lopamudra Giri
    Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA, USA
    J Gen Virol 93:389-99. 2012
    ..Specifically, PFGE was used to detect defective genomes, determine defective genome sizes and quantify the amount of defective genome within a heterogeneous genome population of passaged virus...
  22. pmc Persistence versus reversion of 3TC resistance in HIV-1 determine the rate of emergence of NVP resistance
    Barbara A Rath
    Center for AIDS Research, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Viruses 4:1212-34. 2012
    ..Multidrug pressure experiments lend understanding to mechanisms of HIV resistance as they bear upon new treatment strategies...
  23. ncbi Serial passage of a street rabies virus in mouse neuroblastoma cells resulted in attenuation: potential role of the additional N-glycosylation of a viral glycoprotein in the reduced pathogenicity of street rabies virus
    Kentaro Yamada
    Research Promotion Project, Oita University, 1 1 Idaigaoka, Hasama machi, Yufu, Oita 879 5593, Japan
    Virus Res 165:34-45. 2012
    ..Taken together, we propose that the number of N-glycosylation sites in the G protein is one of the determinants of the pathogenicity of street rabies viruses...
  24. ncbi Serial passage of the opportunistic pathogen Aspergillus flavus through an insect host yields decreased saprobic capacity
    Lisa R Scully
    Department of Biological Sciences, Brock University, St Catharines, ON L2S 3A1, Canada
    Can J Microbiol 51:185-9. 2005
    ..Propagation of the opportunist A. flavus through the insect host G. mellonella resulted in a diminished capacity to grow on an alternate substrate, while maintaining or increasing its ability to use the host as a nutrient supply...
  25. pmc Selective incorporation of influenza virus RNA segments into virions
    Yutaka Fujii
    Department of Virology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734 8551, Japan
    Proc Natl Acad Sci U S A 100:2002-7. 2003
    ..The neuraminidase vRNA incorporation signal and others yet to be identified should provide attractive targets for the attenuation of influenza viruses in vaccine production and the design of new antiviral drugs...
  26. pmc In vitro serial passage of Staphylococcus aureus: changes in physiology, virulence factor production, and agr nucleotide sequence
    Greg A Somerville
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases NIH, 903 South 4th Street, Hamilton, Montana 59840, USA
    J Bacteriol 184:1430-7. 2002
    ..Aconitase specific activity decreased 38% (P < 0.001) by the sixth week in culture. During serial passage, S...
  27. pmc The polymerase acidic protein gene of influenza a virus contributes to pathogenicity in a mouse model
    Min Suk Song
    College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 361 763, Republic of Korea
    J Virol 83:12325-35. 2009
    ..To our knowledge, this is the first study that the isoleucine residue at position 97 in PA plays a key role in enhanced virulence in mice and is implicated in the adaptation of avian influenza viruses to mammalian hosts...
  28. pmc New vaccine design based on defective genomes that combines features of attenuated and inactivated vaccines
    Teresa Rodríguez-Calvo
    Centro de Investigacion en Sanidad Animal, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Madrid, Spain
    PLoS ONE 5:e10414. 2010
    ....
  29. ncbi Subclinical scrapie infection in a resistant species: persistence, replication, and adaptation of infectivity during four passages
    Richard Race
    Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, 903 S Fourth Street, Hamilton, MT 59840, USA
    J Infect Dis 186:S166-70. 2002
    ..In some cases infectivity similar to the original 263K hamster scrapie strain was found after 2 or 3 serial mouse passages totaling 1200-1550 days...
  30. pmc A single-amino-acid substitution in the HA protein changes the replication and pathogenicity of the 2009 pandemic A (H1N1) influenza viruses in vitro and in vivo
    Lili Xu
    Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences CAMS and Comparative Medicine Center, Peking Union Medical Collage PUMC, Beijing 100021, China
    Virol J 7:325. 2010
    ....
  31. ncbi Cellular localization and expression of gp63 homologous metalloproteases in Leishmania (Viannia) braziliensis strains
    Patricia Cuervo
    Laboratório de Pesquisa em Leishmanioses, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil
    Acta Trop 106:143-8. 2008
    ..V.) braziliensis isolates remain unaltered during prolonged in vitro culture, suggesting that the proteolytic activity pattern is a stable phenotypic characteristic of these parasites...
  32. pmc Multiple-hit inhibition of infection by defective interfering particles
    Kristen A Stauffer Thompson
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, Madison, WI 53706 1607, USA
    J Gen Virol 90:888-99. 2009
    ..Our combined experiments and models provide a means to understand better how DIPs quantitatively impact the growth of viruses and the spread of their infections...
  33. ncbi Identification of hepatitis C virus genotype 2a replicon variants with reduced susceptibility to ribavirin
    Su Su Hmwe
    Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Antiviral Res 85:520-4. 2010
    ..However, potential mechanisms of RBV resistance during HCV replication remain poorly understood. Serial passage of cells harboring HCV genotype 2a replicon in the presence of RBV resulted in the reduced susceptibility of ..
  34. ncbi Genetic comparisons between lentogenic Newcastle disease virus isolated from waterfowl and velogenic variants
    Ryota Tsunekuni
    Avian Influenza Research Centre, Kyoto Sangyo University, Kamigamo Motoyama, Kyoto 603 8555, Japan
    Virus Genes 40:252-5. 2010
    ..These results suggest that in addition to the F cleavage site sequence, these two amino acids in HN protein are also related to the pathogenicity of NDV in chickens...
  35. pmc Characterization of mosquito-adapted West Nile virus
    Alexander T Ciota
    The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, 5668 State Farm Road, Slingerlands, NY 12159, USA
    J Gen Virol 89:1633-42. 2008
    ....
  36. pmc Two mechanisms of soluble CD4 (sCD4)-mediated inhibition of human immunodeficiency virus type 1 (HIV-1) infectivity and their relation to primary HIV-1 isolates with reduced sensitivity to sCD4
    S L Orloff
    Immunology Branch, Centers for Disease Control, Atlanta, Georgia 30333
    J Virol 67:1461-71. 1993
    ..Rather, an event that occurs after binding may account for the differences. This postbinding event or feature may be determined by regions of the envelope outside the CD4 binding site...
  37. ncbi Nonpathogenic CCR2-tropic SIVrcm after serial passage and its effect on SIVmac infection of Indian rhesus macaques
    Binhua Ling
    Division of Comparative Pathology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433, USA
    Virology 379:38-44. 2008
    ..These results suggest that SIVrcm is nonpathogenic and may be useful for examining effective immune responses in SIV infection...
  38. pmc Foot-and-mouth disease virus mutant with decreased sensitivity to ribavirin: implications for error catastrophe
    Macarena Sierra
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, E 28049 Madrid, Spain
    J Virol 81:2012-24. 2007
    ..Implications for lethal mutagenesis are discussed...
  39. ncbi Phase 1 studies of Walter Reed Army Institute of Research candidate attenuated dengue vaccines: selection of safe and immunogenic monovalent vaccines
    N Kanesa-Thasan
    Walter Reed Army Institute of Research, Washington, District of Columbia, USA
    Am J Trop Med Hyg 69:17-23. 2003
    ..the results of initial safety testing of 10 live-attenuated dengue virus (DENV) vaccine candidates modified by serial passage in primary dog kidney (PDK) cells at the Walter Reed Army Institute of Research...
  40. pmc High fidelity of yellow fever virus RNA polymerase
    Konstantin V Pugachev
    Acambis, Inc, Cambridge, Massachusetts 02139, USA
    J Virol 78:1032-8. 2004
    ....
  41. pmc Recombinant chimeric yellow fever-dengue type 2 virus is immunogenic and protective in nonhuman primates
    F Guirakhoo
    OraVax, Inc, Cambridge, Massachusetts 02139, USA
    J Virol 74:5477-85. 2000
    ..The high replication efficiency, attenuation phenotype in mice and monkeys, immunogenicity and protective efficacy, and genomic stability of ChimeriVax-D2 justify it as a novel vaccine candidate to be evaluated in humans...
  42. ncbi Characterization of a simian human immunodeficiency virus encoding the envelope gene from the CCR5-tropic HIV-1 Ba-L
    Ranajit Pal
    Advanced BioScience Laboratories, Kensington, Maryland, USA
    J Acquir Immune Defic Syndr 33:300-7. 2003
    ..After serial passage through 4 pigtails by blood and bone marrow transfer, virus from pigtail PBMCs had higher in vitro infectious ..
  43. pmc Characterization of a human H5N1 influenza A virus isolated in 2003
    Kyoko Shinya
    Department of Virology, Institute of Medical Science, University of Tokyo, Japan
    J Virol 79:9926-32. 2005
    ..This case challenges the view that adaptation to land-based poultry is a prerequisite for the replication of aquatic avian influenza A viruses in humans...
  44. pmc Few mutations in the 5' leader region mediate fitness recovery of debilitated human immunodeficiency type 1 viruses
    Eloisa Yuste
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, Universidad Autonoma, Cantoblanco, Madrid, Spain
    J Virol 79:5421-7. 2005
    ....
  45. ncbi A segmented form of foot-and-mouth disease virus interferes with standard virus: a link between interference and competitive fitness
    Juan García-Arriaza
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Virology 335:155-64. 2005
    b>Serial passage of foot-and-mouth disease virus (FMDV) in BHK-21 cells at high multiplicity of infection resulted in dominance of particles containing defective RNAs that were infectious by complementation in the absence of standard viral ..
  46. ncbi E/NS1 modifications of dengue 2 virus after serial passages in mammalian and/or mosquito cells
    Wei June Chen
    Department of Public Health and Parasitology, Chang Gung University, Tao Yuan, Taiwan
    Intervirology 46:289-95. 2003
    ..The number of dengue virus lineages has been increasing over time. The aim of this study was to identify the genetic diversity of dengue 2 virus serially transferred in mammalian and/or mosquito cells...
  47. pmc Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo
    Cecilia Tami
    Instituto de Biotecnologia, Centro de Investigación en Ciencias Veterinarias y Agronómicas, INTA, Buenos Aires, Argentina
    J Virol 77:1219-26. 2003
    ..These results suggest that the selective pressure exerted by host humoral immune response can play a role in both the selection and stability of antigenic FMDV variants and that such variants can manifest alterations in cell tropism...
  48. ncbi Efficient production of infectious hepatitis C virus with adaptive mutations in cultured hepatoma cells
    Yasuaki Bungyoku
    Division of Microbiology, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe, Hyogo 650 0017, Japan
    J Gen Virol 90:1681-91. 2009
    ..The J6/JFH1-derived mutant viruses presented here would be a good tool for producing HCV particles with enhanced infectivity and for studying the molecular mechanism of HCV entry...
  49. pmc Multiple factors interact to produce responses resembling spectrum of human disease in Campylobacter jejuni infected C57BL/6 IL-10-/- mice
    Julia A Bell
    Comparative Enteric Diseases Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA
    BMC Microbiol 9:57. 2009
    ..We took a comparative experimental approach to explore differences among different C. jejuni strains and studied the effect of diet on disease manifestation in an interleukin-10 deficient mouse model...
  50. ncbi Altered pathogenicity, immunogenicity, tissue tropism and 3'-7kb region sequence of an avian infectious bronchitis coronavirus strain after serial passage in embryos
    Shengwang Liu
    Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, PR China
    Vaccine 27:4630-40. 2009
    ..we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs...
  51. pmc Role of the mutant spectrum in adaptation and replication of West Nile virus
    Alexander T Ciota
    Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA
    J Gen Virol 88:865-74. 2007
    ..These data provide evidence of the importance of quasispecies dynamics in the adaptation of a flavivirus to new and changing environments and hosts, with little evidence of significant genetic change...
  52. ncbi Preparation and characterization of new challenge stocks of SIVmac32H J5 following rapid serial passage of virus in vivo
    D Ferguson
    Division of Retrovirology, NIBSC, South Mimms, Potters Bar, Hertfordshire, UK
    J Med Primatol 36:131-42. 2007
    ..A new challenge stock of the simian immunodeficiency virus SIVmacJ5 has been produced following passage in vivo...
  53. ncbi The anti-yellow fever virus activity of ribavirin is independent of error-prone replication
    Pieter Leyssen
    Rega Institute for Medical Research, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Mol Pharmacol 69:1461-7. 2006
    ..These data thus allow us to conclude that the in vitro anti-yellow fever virus activity of ribavirin is independent of error-prone replication...
  54. ncbi Plasmodium vinckei: selection of a strain exhibiting stable resistance to arteether
    S K Puri
    Division of Parasitology, Central Drug Research Institute, Lucknow 226001, India
    Exp Parasitol 114:129-32. 2006
    ..Cross-sensitivity studies have shown that apart form resistance to related derivatives like artemether and artesunic acid, the derived parasites also show resistance to quinine and mefloquine...
  55. ncbi Genetic stability of the attachment glycoprotein of human respiratory syncytial viruses during serial passages in cell cultures
    M de Sierra
    Seccion Virologia, Facultad de Ciencias, Universidad de la Republica, Igua 4225 11400, Montevideo, Uruguay
    Acta Virol 48:115-21. 2004
    ..All the isolates analyzed showed no changes during passaging in HEp-2 cells...
  56. pmc A single amino acid change in the C-terminal domain of the matrix protein M1 of influenza B virus confers mouse adaptation and virulence
    Jonathan A McCullers
    Department of Infectious Diseases, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105 2794, USA
    Virology 336:318-26. 2005
    b>Serial passage of an initially avirulent influenza B virus, B/Memphis/12/97, resulted in the selection of a variant which was lethal in mice. Virulence correlated with improved growth in vivo and prolonged replication...
  57. pmc Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture
    Angelina Plyusnina
    Haartman Institute, Department of Virology, University of Helsinki POB 21, FIN 00014, Helsinki, Finland
    Virol J 2:12. 2005
    ..The observed survival time seems to be sufficient for transmission of newly formed recombinant hantaviruses in nature...
  58. pmc Mutational dynamics of the SARS coronavirus in cell culture and human populations isolated in 2003
    Vinsensius B Vega
    Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672
    BMC Infect Dis 4:32. 2004
    ..The recent emergence of this new pathogen, the careful tracing of its transmission patterns, and the ability to propagate in culture allows the exploration of the mutational dynamics of the SARS-CoV in human populations...
  59. pmc Two amino acid mutations in the capsid protein of type 2 porcine circovirus (PCV2) enhanced PCV2 replication in vitro and attenuated the virus in vivo
    M Fenaux
    Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1410 Price s Fork Rd, Blacksburg, VA 24061 0342, USA
    J Virol 78:13440-6. 2004
    ..This finding has important implications for PCV2 vaccine development...
  60. pmc Cell-line-induced mutation of the rotavirus genome alters expression of an IRF3-interacting protein
    Karen Kearney
    Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    EMBO J 23:4072-81. 2004
    ..These data demonstrate that host cell type and propagation conditions affect the capacity of RRV to produce the virulence gene product NSP1, an important consideration in producing RRV-based vaccines...
  61. ncbi Production, characterization and evaluation of virulence of an adhesion defective mutant of Flavobacterium columnare produced by beta-lactam selection
    J A Bader
    Aquatic Animal Health Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Auburn, AL 36831 0952, USA
    Lett Appl Microbiol 40:123-7. 2005
    ..The aim of this study was to develop and evaluate mutants of Flavobacterium columnare, the causative agent of columnaris disease in fish...
  62. ncbi Mutations in Turnip mosaic virus genomes that have adapted to Raphanus sativus
    Zhongyang Tan
    Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840 8502, Japan
    J Gen Virol 86:501-10. 2005
    ..It seems likely that this site is important in the initial stages of adaptation to R. sativus. Other independent substitutions were mostly found in the P3, CI and VPg genes...
  63. pmc Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2
    Joseph E Blaney
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 USA
    BMC Infect Dis 4:39. 2004
    ..To prepare a vaccine candidate, a previously described 30 nucleotide deletion (Delta30) in the 3' untranslated region of DEN-4 has been engineered into the DEN-2 isolate...
  64. ncbi Genetic characterization of a mumps virus isolate during passaging in the amniotic cavity of embryonated chicken eggs
    Jelena Ivancic
    Molecular Biomedicine Unit, Department of Research and Development, Institute of Immunology Inc, Rockefellerova 10, 10 000, Zagreb, Croatia
    Virus Res 99:121-9. 2004
    ..We discuss whether the observed differences between the two variants are a consequence of natural heterogeneity or of laboratory contamination in the early passages...
  65. pmc Characterization of in vitro-generated and clinical optochin-resistant strains of Streptococcus pneumoniae isolated from Argentina
    Paulo R Cortes
    Departamento de Bioquimica Clinica, CIBICI, CONICET, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Medina Allende esq Haya de la Torre, Ciudad Universitaria, X5000HUA Córdoba, Argentina
    J Clin Microbiol 46:1930-4. 2008
    ..pneumoniae and may be carried by circulating pneumococcal strains...
  66. ncbi Mutations in the NS2B and NS3 genes affect mouse neuroinvasiveness of a Western European field strain of tick-borne encephalitis virus
    Daniel Ruzek
    Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branisovska 31, CZ 370 05 Ceske Budejovice, Czech Republic
    Virology 374:249-55. 2008
    ..The significance of these observations is discussed...
  67. pmc Wild type measles virus attenuation independent of type I IFN
    Johan Druelle
    INSERM, U758, Lyon, F 69365 France
    Virol J 5:22. 2008
    ..Measles virus attenuation has been historically performed by adaptation to cell culture. The current dogma is that attenuated virus strains induce more type I IFN and are more resistant to IFN-induced protection than wild type (wt)...
  68. pmc Generation of biologically contained Ebola viruses
    Peter Halfmann
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 105:1129-33. 2008
    ....
  69. ncbi Identical rearrangement of NSP3 genes found in three independently isolated virus clones derived from mixed infection and multiple passages of Rotaviruses
    M M Alam
    Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
    Arch Virol 153:555-9. 2008
    ..These findings suggest that rearrangement of the NSP3 gene may occur at a certain preferential site which is related to sequence similarity between 3'-untranslated region and a region near the 5'-end of ORF...
  70. ncbi Study of the genetic stability of measles virus CAM-70 vaccine strain after serial passages in chicken embryo fibroblasts primary cultures
    Maria Beatriz Borges
    Bio Manguinhos, Fundacao Oswaldo Cruz, Av Brasil, 4365 Manguinhos, Rio de Janeiro, RJ 21040 360, Brazil
    Virus Genes 36:35-44. 2008
    ..This study shows that the measles CAM-70 vaccine virus is highly adapted to its cultivation conditions and that its genetic stability contributes, in part, to the safety profile of the vaccine...
  71. ncbi Sequence changes of infectious bronchitis virus isolates in the 3' 7.3 kb of the genome after attenuating passage in embryonated eggs
    Yuan Pin Huang
    Department of Veterinary Medicine, National Taiwan University No 1, Sec 4, Roosevelt Road, Taipei 10617, Taiwan
    Avian Pathol 36:59-67. 2007
    ..This study is the first demonstration for IBV comparing sequence changes in the 3' 7.3 kb genome after attenuation. The aforementioned information on amino acid changes might be useful in future virulence studies...
  72. pmc Participation of both host and virus factors in induction of severe acute respiratory syndrome (SARS) in F344 rats infected with SARS coronavirus
    Noriyo Nagata
    Department of Pathology, National Institute of Infectious Diseases, Gakuen 4 7 1, Musashimurayama, Tokyo 208 0011, Japan
    J Virol 81:1848-57. 2007
    ..These results strongly indicate that host and virus factors such as advanced age and virus adaptation are critical for the development of SARS in rats...
  73. pmc Rational design of genetically stable, live-attenuated poliovirus vaccines of all three serotypes: relevance to poliomyelitis eradication
    Andrew J Macadam
    NIBSC, South Mimms, Herts, UK
    J Virol 80:8653-63. 2006
    ....
  74. pmc Rapid adaptation of a recombinant vesicular stomatitis virus to a targeted cell line
    Yanhua Gao
    Department of Pediatrics, University of Pittsburgh School of Medicine, PA 15213, USA
    J Virol 80:8603-12. 2006
    ..We performed in vitro serial passage in D2F2/E2 cells to evolve a virus with improved growth that could be used for preclinical therapy trials in ..
  75. ncbi Characterization of homologous defective interfering RNA during persistent infection of Vero cells with Japanese encephalitis virus
    Sung Wook Yoon
    Department of Biology and Research Institute of Basic Sciences, Kyung Hee University, Seoul 130 701, Korea
    Mol Cells 21:112-20. 2006
    ..Taken together, these ob-servations suggest that the generation of homologous DI RNA during successive JEV acute infections in Vero cells probably participates actively in persistent JEV infection...
  76. pmc Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution
    Atze T Das
    Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam CINIMA, Academic Medical Center of the University of Amsterdam, The Netherlands
    Retrovirology 5:44. 2008
    ..Since macaque studies will likely require an efficiently replicating variant, we set out to optimize SIV-rtTA through in vitro viral evolution...
  77. pmc Inhibition, escape, and attenuated growth of severe acute respiratory syndrome coronavirus treated with antisense morpholino oligomers
    Benjamin W Neuman
    The Scripps Research Institute, Division of Virology, Department of Neuropharmacology, La Jolla, CA 92037, USA
    J Virol 79:9665-76. 2005
    ..Those partially resistant viruses grew more slowly and formed smaller plaques than wild-type SARS-CoV. These results suggest PMO compounds have powerful therapeutic and investigative potential toward coronavirus infection...
  78. ncbi Emergence of mutation clusters in the HCV genome during sequential viral passages in Sip-L expressing cells
    Der Ren Hwang
    Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Taipei, Taiwan
    J Virol Methods 129:170-7. 2005
    ..The mutant HCV can be used as an infection source to study the molecular mechanism of HCV replication...
  79. ncbi A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) is genetically stable in vitro and in vivo
    J Gillespie
    Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 0342, USA
    Vaccine 26:4231-6. 2008
    ..The results indicate that the PCV1-2 chimera virus is genetically stable, and thus should be a good vaccine candidate...
  80. pmc Modifications of the 3'-UTR stem-loop of infectious bursal disease virus are allowed without influencing replication or virulence
    Hein J Boot
    Animal Sciences Group, Wageningen University and Research Centre, Lelystad, The Netherlands
    Nucleic Acids Res 32:211-22. 2004
    ..Apparently, it is the stem-loop structure and not the primary sequence that is the functional determinant in the 3'-UTRs of IBDV...
  81. pmc Determinants of increased replicative capacity of serially passaged simian immunodeficiency virus with nef deleted in rhesus monkeys
    Louis Alexander
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut, USA
    J Virol 77:6823-35. 2003
    ..These results delineate sequence changes in SIV that can compensate for the loss of the nef gene to partially restore replicative and pathogenic potential in rhesus monkeys...
  82. ncbi Phenotypic and genotypic characterization of the neurovirulence and neuroinvasiveness of a large-plaque attenuated Japanese encephalitis virus isolate
    Suh Chin Wu
    Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC
    Microbes Infect 5:475-80. 2003
    ..Elucidating the molecular basis of virulence of flaviviruses can provide valuable information for live-attenuated vaccine development...
  83. ncbi Resistance to extinction of low fitness virus subjected to plaque-to-plaque transfers: diversification by mutation clustering
    Cristina Escarmis
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, Universidad Autonoma de Madrid, 28049, Spain
    J Mol Biol 315:647-61. 2002
    ..The results document resistance of viral clones to extinction, and suggest that mutation clustering may be a mechanism of genetic diversification of low fitness virus...
  84. pmc Covariation in the capsid protein of hibiscus chlorotic ringspot virus induced by serial passaging in a host that restricts movement leads to avirulence in its systemic host
    Xiao Zhen Liang
    Department of Biological Sciences, The National University of Singapore, Singapore 117543, Republic of Singapore
    J Virol 76:12320-4. 2002
    ..Interestingly, the nature of the covariation was consistent and reproducible at each serial passage. These data indicate that the nonsynonymous substitutions of amino acids in the HCRSV CP after serial passages ..
  85. ncbi Molecular analysis of the capsid coding region of a virulent encephalomyocarditis virus isolate after serial cell passages and assessment of its virulence
    P Denis
    Veterinary and Agrochemical Research Center, Brussels, Belgium
    Arch Virol 148:903-12. 2003
    ..Results confirm the ability of EMCV to become attenuated after passages in cell culture...
  86. ncbi Reduced fitness of HIV-1 resistant to CXCR4 antagonists
    Mercedes Armand-Ugon
    Retrovirology Laboratory irsiCaixa, Hospital Universitari Germans Trias i Pujol, Universitat Autonoma de Barcelona, 08916 Badalona, Spain
    Antivir Ther 8:1-8. 2003
    ..Thus, viral resistance development to a CXCR4 antagonist such as AMD3100 is associated with reduced viral fitness...
  87. ncbi In vitro and in vivo expression of foreign genes by transmissible gastroenteritis coronavirus-derived minigenomes
    Sara Alonso
    Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia, CSIC, Campus Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
    J Gen Virol 83:567-79. 2002
    ..The large cloning capacity and the tissue specificity of the TGEV-derived minigenomes suggest that these virus vectors are very promising for vaccine development...
  88. pmc A few-polyhedra mutant and wild-type nucleopolyhedrovirus remain as a stable polymorphism during serial coinfection in Trichoplusia ni
    James C Bull
    Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom
    Appl Environ Microbiol 69:2052-7. 2003
    Few-polyhedra (FP) mutants of nucleopolyhedroviruses (NPVs) are a well-known phenomenon during serial passage of virus in cell culture...
  89. pmc Changes in rhinovirus protein 2C allow efficient replication in mouse cells
    Julie R Harris
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Virol 77:4773-80. 2003
    ..The characterization of a rhinovirus host range mutant will facilitate the investigation of cellular proteins required for efficient viral growth and the development of a murine model for rhinovirus infection...
  90. ncbi Identification of mutations associated with attenuation of virulence of a field Sendai virus isolate by egg passage
    Yutaka Fujii
    Department of Bacteriology, Hiroshima University School of Medicine, Japan
    Virus Genes 25:189-93. 2002
    ..The significance of the mutations detected in the leader as well as in the L and HN genes was discussed in the context of attenuation of SeV pathogenicity by egg passage...
  91. ncbi Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains
    S Yuan
    Department of Veterinary Pathobiology, University of Minnesota, St. Paul, MN 55108, USA
    Virus Res 74:99-110. 2001
    ..The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system...
  92. ncbi Enhanced green fluorescent protein expression may be used to monitor murine coronavirus spread in vitro and in the mouse central nervous system
    Jayasri Das Sarma
    Department of Physiology, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Neurovirol 8:381-91. 2002
    ..Thus, EGFP-expressing viruses will be useful in the studies of murine coronavirus pathogenesis in mice...
  93. ncbi Importance of amino acid 216 in nonstructural protein 2B for replication of hepatitis A virus in cell culture and in vivo
    Judith Graff
    Molecular Hepatitis Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infections Diseases, National Institutes of Health, Bethesda, Maryland 20892 8009, USA
    J Med Virol 71:7-17. 2003
    ..Attempts to complement wild-type 2B in trans with adapted 2B provided by co-infection with a second viable HAV mutant failed to enhance replication of the virus containing the wild-type 2B sequence...
  94. pmc Sequence requirements for Sindbis virus subgenomic mRNA promoter function in cultured cells
    M M Wielgosz
    Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110-1093, USA
    J Virol 75:3509-19. 2001
    ..The promoter thus consists of an essential central region from -15 to +5 and discrete flanking sites that render it fully active, depending on the host environment...
  95. pmc Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA
    Yutaka Fujii
    Department of Virology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734 8551, Japan
    J Virol 76:8540-7. 2002
    ..These findings suggest that leader mutations of SeV affect virus pathogenesis by altering virus replication in a host-dependent manner...
  96. ncbi Translation efficiency determines differences in cellular infection among dengue virus type 2 strains
    Dianna Edgil
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720 7360, USA
    Virology 317:275-90. 2003
    ..Thus, differences in infectivity among closely related DEN2 strains correlate with efficiency of translation of input viral RNA...
  97. pmc Modeling viral genome fitness evolution associated with serial bottleneck events: evidence of stationary states of fitness
    Ester Lázaro
    Centro de Astrobiologia CSIC INTA, 28850 Torrejon de Ardoz, Madrid, Spain
    J Virol 76:8675-81. 2002
    ..In particular, the size of the bottleneck is critical for determining the trend of fitness evolution...
  98. pmc Changes in both gp120 and gp41 can account for increased growth potential and expanded host range of human immunodeficiency virus type 1
    K Fujita
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892
    J Virol 66:4445-51. 1992
    ..They also may have important implications for the generation of viruses with increased growth potential and expanded host range seen in the late stages of HIV disease...
  99. pmc Selection of an infectious bursal disease virus mutant with increased immunogenicity following passage under humoral immune pressure
    M M Nagarajan
    Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown
    Can J Vet Res 65:89-96. 2001
    ..capability, but showing similar growth characteristics as the parental virus strain, QC2, was isolated after serial passage in Vero cells in presence of IBDV serotype 1 chicken polyclonal antiserum...
  100. pmc The number of a U3 repeat box acting as an enhancer in long terminal repeats of polytropic replication-competent porcine endogenous retroviruses dynamically fluctuates during serial virus passages in human cells
    G Scheef
    Paul-Ehrlich-Institut, D-63225 Langen, Germany
    J Virol 75:6933-40. 2001
    ..During serial virus passaging of molecular PERV clones on human 293 cells, proviral LTRs demonstrated adaptation of transcriptional activity by dynamic changes of the number of 39-bp repeats in the course of up to 12 passaging cycles...
  101. pmc Propagation of rat parvovirus in thymic lymphoma cell line C58(NT)d and subsequent appearance of a resistant cell clone after lytic infection
    Y Ueno
    Institute of Basic Medical Sciences and Laboratory Animal Research Center, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan
    J Virol 75:3965-70. 2001
    ..Interestingly, a resistant subclone, C58(NT)D/R, from surviving cells after lytic infection had differentiated phenotypic modifications, such as increased cell adherence, resistance to apoptosis, and suppressed tumorigenicity...

Research Grants53

  1. Optimized Adaptation of Simian-tropic R5 HIV Clade C to Pig-tailed Macaques
    RUTH MARGRIT RUPRECHT; Fiscal Year: 2013
    ..Further adapt progeny virus by serial passage through immunocompetent PMs, re-isolate virus from the last recipient, generate a large stock, and ..
  2. Infant immunoprophylaxis against a primate lentivirus
    RUTH MARGRIT RUPRECHT; Fiscal Year: 2012
    ..R5 SHIV-clade A and SHIV-clade D viruses will be adapted to rhesus monkeys by rapid serial passage. We will take advantage of the optimized proviral backbones we created for our SHIV-clade C, which was ..
  3. Development of a Novel Rift Valley Fever Virus Vaccine
    Shinji Makino; Fiscal Year: 2013
    ..MP-12 strain, which was developed by 12 times serial passage of the wild-type RVFV strain ZH548, is markedly attenuated for mice, yet retains immunogenicity...
  4. Identification of novel HIV capsid assembly inhibitors (Hit-to-Lead development)
    OSVALDO ARIEL FLORES; Fiscal Year: 2011
    ..as resistance selection of these compounds to confirm that mutations in resistant viruses selected through serial passage, map to the capsid protein sites of assembly...
  5. Streptococcus pneumoniae genetic competence and pathogenesis
    Michael E Sebert; Fiscal Year: 2011
    ..Evidence for mutation accumulation will be sought using both in vitro and in vivo serial passage models and measuring the fitness of evolved lineages...
  6. A Novel Strategy for Recombinant Adeno-Associated Virus Vector Production
    Patrick Hearing; Fiscal Year: 2013
    ..We used computational engineering to generate a Rep gene that is stably maintained over serial passage in a recombinant Ad vector...
  7. Role of precipitous virulence in invasiveness of community-acquired methicillin-r
    Victor J Torres; Fiscal Year: 2013
    ..and 3) evaluate the relative virulence and fitness of strains with upshifts both in vitro and in vivo using serial passage and competition tests...
  8. Sigma virus in Drosophila: a model system for the evolution of virulence
    Marta L Wayne; Fiscal Year: 2012
    ..animal model system to test the generality of results of virulence evolution from microbial or cell culture serial passage experiments is an essential initial step toward predicting and ultimately managing such evolutionary changes...
  9. Oral Reovirus-Based Vaccines for Prevention of HIV-1 Disease
    Terence S Dermody; Fiscal Year: 2012
    ..MPER-expressing reoviruses will be tested for replication efficiency, stability during serial passage, and interactions with MPER-specific mAbs...
  10. Identifaction of the genetic basis of primaquine resistance in malaria parasites
    ERIKA LEA FLANNERY; Fiscal Year: 2013
    ..berghei. I will then evolve parasite resistance to primaquine by serial passage of blood stages of P. berghei in mice treated with increasing concentrations of primaquine...
  11. Epigenetic regulation of temozolomide responsiveness in glioblastoma
    Jann N Sarkaria; Fiscal Year: 2012
    ..an intracranial xenograft therapy model using a panel of 17 human GBMs that were established and maintained by serial passage in the flank of nude mice...
  12. Serial Passage: AIDS, Race, and Culture Phase II
    CLAUDIA PRYOR; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): Serial Passage: AIDS, Race, and Culture...
  13. HIV-1 Env Microglial Tropism and Neurovirulence
    Ruth Ruprecht; Fiscal Year: 2009
    ..1995), a neuropathogenic simian immunodeficiency virus (SIV) strain was isolated by serial passage of SIVmac251 through infected MG;after 3 such passages, the progeny SIVmac182 reliably induced CNS disease in ..
  14. DEVELOPMENT OF CELL LINES FROM SUBLINGUAL GLANDS
    DAVID CULP; Fiscal Year: 1992
    ..cell line which displays the following characteristics: 1) Proliferate at a rapid rate and undergo serial passage without significant changes in growth characteristics; 2) Undergo induction to express the mucous cell ..
  15. EVOLUTION OF VIRULENCE OF EQUINE INFECTIOUS ANEMIA VIRUS
    Susan Payne; Fiscal Year: 2002
    ..Parallel serial passage experiments will be used to trace changes in levels and patterns of variation that occur with infection of a ..
  16. Microarray Analysis of Leptospiral Genomes
    Joseph Vinetz; Fiscal Year: 2007
    ..interrogans serovar Copenhageni strain L1- 130 genome strain attenuated by serial passage in vitro...
  17. INSULIN RESISTANCE AND CALCIUM METABOLISM IN PREGNANCY
    James Sowers; Fiscal Year: 1993
    ..insulin sensitivity and [Ca2+]i metabolism in skin fibroblasts of women who develop PIH are lost through serial passage of the cells in culture and whether these alterations persist 6 weeks following delivery...
  18. WILDLIFE RESERVOIRS OF ARBOVIRUSES
    THOMAS YUILL; Fiscal Year: 1980
    ..virus strains for the occurrence of temperature sensitive (Ts) mutants, and to determine if mosquito-mosquito serial passage, without intervening vertebrate infection, selects for an increase in the Ts population...
  19. Rift Valley Fever Virus MP-12 Vaccine Completion
    CLARENCE PETERS; Fiscal Year: 2004
    ..immunogenicity, USAMRIID created a live vaccine designated MP12, which was mutagenized and attenuated by serial passage in the presence of 5-fluorouracil with subsequent plaque cloning and amplification...
  20. Regulation of Viral Membrane Fusion
    Richard Compans; Fiscal Year: 2003
    ..It will also be determined whether serial passage of SER virus results in the appearance of fusogenic variants with a truncated cytoplasmic tail, and whether ..
  21. Determinants of host adaptation in a Macaca nemestrina-adapted HIV-2 isolate
    Gregory Brennan; Fiscal Year: 2009
    ..We hypothesize that the HIV-2/304 phenotype in macaques is due to mutations accumulated during serial passage, which increase viral efficiency and/or evade restriction factors...
  22. SMALL ANIMAL MODEL FOR HEPATITIS C VIRUS REPLICATION
    Linda Andrus; Fiscal Year: 2001
    ..Finally, serial passage of virus from infected to non-infected transplanted animals will be performed to provide definitive proof of ..
  23. RADIOPROTECTIVE EFFECT OF MAMMARY TUMOR CELL GRAFTS
    Charles Scarantino; Fiscal Year: 1980
    ..The extent of the donor contribution to the stem cell component of the tumor will be determined by serial passage of mammary tumor cells in stem cell-deficient recipients and by appropriate in vitro cell segregation methods...
  24. Safety and Efficacy of SHIV Vaccine in Macaques
    Opendra Narayan; Fiscal Year: 2006
    ..studies established that the vaccine is safe for at least 4 years after inoculation into newborns and after serial passage in young macaques...
  25. Modulation of HIV-1 Replication and T Cell Activation by FoxP3
    Lishan Su; Fiscal Year: 2007
    ..4) The PI (Su-UNC), co-Pi (Zhang-UNC) and collaborators (Baldwin-UNC and Fu-UCSD) form a team with complementary expertise to address the questions with different perspectives/resources. ..
  26. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  27. POPULATION BIOLOGY OF BACTERIAL VIRUSES AND PLASMIDS
    Bruce Levin; Fiscal Year: 2002
    ....
  28. POPULATION GENETICS & EVOLUTION OF ANTIBIOTIC RESISTANCE
    Bruce Levin; Fiscal Year: 2008
    ..abstract_text> ..
  29. Experimental Creutzfeldt Jacob Disease
    Laura Manuelidis; Fiscal Year: 2007
    ..Rapid infectivity assays in vitro can open the study of TSEs by providing a way to test any pure molecule or structure for its intrinsic infectivity by Koch's established postulates for infectious pathogens. ..
  30. Complement mediated lysis resistance genes of Leishmania
    JEFFREY BEETHAM; Fiscal Year: 2008
    ..abstract_text> ..
  31. Senescence bypass in epithelial neoplastic progression
    JAMES RHEINWALD; Fiscal Year: 2008
    ..abstract_text> ..
  32. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  33. Animal models to design & evaluate improved VZV vaccines
    WAYNE GRAY; Fiscal Year: 2007
    ..The study may also provide support for use of the VZV vaccine as a recombinant vector for immunization against other infectious agents, particularly human immunodeficiency virus (HIV). ..
  34. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  35. Novel genetic approach to dissecting homing /engraftment
    Lishan Su; Fiscal Year: 2004
    ..The novel (Degrakine) genetic approach will also be applicable to the analysis of cell migration in other biological processes such as heart and lung functions, as well as mature hemato-lymphoid cells. ..
  36. RHOA EFFECTOR IN HIV-1 REPLICATION AND PATHOGENESIS
    Lishan Su; Fiscal Year: 2004
    ..abstract_text> ..
  37. Sterol Biosynthesis in Trypanosomatid Parasites
    Frederick Buckner; Fiscal Year: 2004
    ..The drugs discovered in this research program will hopefully provide better future treatment for patients with these devastating diseases. ..
  38. RAP1A AND RAP1B IN THE DIAGNOSIS AND TMT - ORAL CANCER
    NISHA D SILVA; Fiscal Year: 2004
    ..The third segment involves the design of a protocol to investigate the use of rap1A in gene therapy to treat residual epithelial dysplasia or squamous cell carcinoma lesions. ..
  39. Genetic Analy. of Chemokine Receptors in T Cell Develop.
    Lishan Su; Fiscal Year: 2003
    ..abstract_text> ..
  40. SEQUENCE SELECTION AND PERSISTENCE OF HEPATITIS C
    Stuart Ray; Fiscal Year: 2003
    ..This application is efficient and has a high likelihood of success because it makes use of a well-characterized cohort, for which years of clinical and virologic data have already been assembled. ..
  41. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  42. Hepatitis C Recombination Incidence and Prevalence
    Stuart Ray; Fiscal Year: 2004
    ..Because the impact of HCV recombination has not yet been determined, R21 funding will provide adequate preliminary data to establish the importance and proper conduct of more extensive investigations. ..
  43. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  44. Activation & Proliferation of Hematopoietic Stem Cells
    Peter Lansdorp; Fiscal Year: 2006
    ..Such information is relevant for a basic understanding of hematopoiesis as well as applications of stem cells in and outside hematology. ..
  45. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
  46. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
  47. Filovirus/cellular receptor interactions
    Wendy Maury; Fiscal Year: 2007
    ..Development of equivalent drugs for the filoviruses might prove to be successful in limiting new outbreaks. [unreadable] [unreadable]..
  48. Rap 1-Mediated Protein Transport in Oral Cancer
    NISHA D SILVA; Fiscal Year: 2006
    ..The significance of this research is that it explores an important and novel mechanism for nucleocytoplasmic transport of pro-proliferative proteins in oral cancer, thereby identifying novel treatment targets. [unreadable] [unreadable]..
  49. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  50. HIV-1 Replication and Pathogenesis in the Human Thymus
    Lishan Su; Fiscal Year: 2007
    ..abstract_text> ..
  51. Junction Adhesion Molecule CAR and the Immune System
    Steven Carson; Fiscal Year: 2007
    ..This work will advance knowledge of cells that form tight _junctions and their interactions with cells and molecules that must cross those barriers. ..
  52. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  53. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..