bacteriological techniques

Summary

Top Publications

  1. pmc Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology
    Danica Helb
    Department of Medicine, New Jersey Medical School, University of Medicine and Dentistry, New Jersey, Newark, New Jersey 07103, USA
    J Clin Microbiol 48:229-37. 2010
  2. ncbi Sonication of removed hip and knee prostheses for diagnosis of infection
    Andrej Trampuz
    Division of Infectious Diseases, Department of Internal Medicine, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    N Engl J Med 357:654-63. 2007
  3. ncbi Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review
    Karen R Steingart
    Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital, University of California, San Francisco, CA, USA
    Lancet Infect Dis 6:664-74. 2006
  4. ncbi Liquid vs. solid culture for tuberculosis: performance and cost in a resource-constrained setting
    V N Chihota
    Aurum Institute for Health Research, Johannesburg, South Africa
    Int J Tuberc Lung Dis 14:1024-31. 2010
  5. ncbi Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    Piseth Seng
    Pôle des Maladies Infectieuses, Assistance Publique Hopitaux de Marseille, URMITE, Unite Mixte de Recherche, Centre National de la Recherche Scientifique Institut pour la Recherche et le Développement 6236, Universite de la Mediterranee, Marseille, France
    Clin Infect Dis 49:543-51. 2009
  6. ncbi Recommendations for diagnosis of shiga toxin--producing Escherichia coli infections by clinical laboratories
    L Hannah Gould
    Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, CDC, Atlanta, Georgia 30333, USA
    MMWR Recomm Rep 58:1-14. 2009
  7. pmc Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry
    Bernard La Scola
    Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille and URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    PLoS ONE 4:e8041. 2009
  8. ncbi Mass spectrometry tools for the classification and identification of bacteria
    Sascha Sauer
    Max Planck Institute for Molecular Genetics, Otto Warburg Laboratory, Ihnestrasse 63 73, D 14195 Berlin, Germany
    Nat Rev Microbiol 8:74-82. 2010
  9. pmc Diagnostic accuracy of a urine lipoarabinomannan test for tuberculosis in hospitalized patients in a High HIV prevalence setting
    Maunank Shah
    Department of Medicine, Johns Hopkins University Center for Tuberculosis Research, Baltimore, MD 21231, USA
    J Acquir Immune Defic Syndr 52:145-51. 2009
  10. pmc Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Lindsay G Stevenson
    Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892 1508, USA
    J Clin Microbiol 48:444-7. 2010

Detail Information

Publications291 found, 100 shown here

  1. pmc Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology
    Danica Helb
    Department of Medicine, New Jersey Medical School, University of Medicine and Dentistry, New Jersey, Newark, New Jersey 07103, USA
    J Clin Microbiol 48:229-37. 2010
    ..2%) susceptible cases. Specificity rose to 100% after correcting for a conventional susceptibility test error. In conclusion, this highly sensitive and simple-to-use system can detect M. tuberculosis directly from sputum in less than 2 h...
  2. ncbi Sonication of removed hip and knee prostheses for diagnosis of infection
    Andrej Trampuz
    Division of Infectious Diseases, Department of Internal Medicine, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    N Engl J Med 357:654-63. 2007
    ..We hypothesized that culturing of samples obtained from the prosthesis would improve the microbiologic diagnosis of prosthetic-joint infection...
  3. ncbi Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review
    Karen R Steingart
    Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital, University of California, San Francisco, CA, USA
    Lancet Infect Dis 6:664-74. 2006
    ..Operational studies are needed to determine whether the increased sensitivity provided by processing methods is sufficient to offset their increased cost, complexity, and potential biohazards, and to examine their feasibility...
  4. ncbi Liquid vs. solid culture for tuberculosis: performance and cost in a resource-constrained setting
    V N Chihota
    Aurum Institute for Health Research, Johannesburg, South Africa
    Int J Tuberc Lung Dis 14:1024-31. 2010
    ..National Health Laboratory Services tuberculosis (TB) laboratory, South Africa...
  5. ncbi Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    Piseth Seng
    Pôle des Maladies Infectieuses, Assistance Publique Hopitaux de Marseille, URMITE, Unite Mixte de Recherche, Centre National de la Recherche Scientifique Institut pour la Recherche et le Développement 6236, Universite de la Mediterranee, Marseille, France
    Clin Infect Dis 49:543-51. 2009
    ..Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry accurately identifies both selected bacteria and bacteria in select clinical situations. It has not been evaluated for routine use in the clinic...
  6. ncbi Recommendations for diagnosis of shiga toxin--producing Escherichia coli infections by clinical laboratories
    L Hannah Gould
    Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, CDC, Atlanta, Georgia 30333, USA
    MMWR Recomm Rep 58:1-14. 2009
    ..Improving the diagnostic accuracy of STEC infection by clinical laboratories should ensure prompt diagnosis and treatment of these infections in patients and increase detection of STEC outbreaks in the community...
  7. pmc Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry
    Bernard La Scola
    Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille and URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    PLoS ONE 4:e8041. 2009
    ..In order to speed up the identification process, in the present work we attempted bacterial identification directly from blood culture bottles detected positive by the automate...
  8. ncbi Mass spectrometry tools for the classification and identification of bacteria
    Sascha Sauer
    Max Planck Institute for Molecular Genetics, Otto Warburg Laboratory, Ihnestrasse 63 73, D 14195 Berlin, Germany
    Nat Rev Microbiol 8:74-82. 2010
    ..In this Review we discuss the application range of these mass spectrometry procedures and their potential for successful transfer into microbiology laboratories...
  9. pmc Diagnostic accuracy of a urine lipoarabinomannan test for tuberculosis in hospitalized patients in a High HIV prevalence setting
    Maunank Shah
    Department of Medicine, Johns Hopkins University Center for Tuberculosis Research, Baltimore, MD 21231, USA
    J Acquir Immune Defic Syndr 52:145-51. 2009
    ..Effective tuberculosis (TB) control in HIV-prevalent settings is hindered by absence of accurate, rapid TB diagnostic tests. We evaluated the accuracy of a urine lipoarabinomannan (LAM) test for TB diagnosis in South Africa...
  10. pmc Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Lindsay G Stevenson
    Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892 1508, USA
    J Clin Microbiol 48:444-7. 2010
    ..This method provides a rapid, accurate, definitive identification of bacteria within 1 h of detection in positive blood cultures with the caveat that the identification of S. pneumoniae would have to be confirmed by an alternative test...
  11. ncbi Novel and improved technologies for tuberculosis diagnosis: progress and challenges
    Madhukar Pai
    Department of Epidemiology, Biostatistics and Occupational Health, McGill University, 1020 Pine Avenue West, Montreal, H3A 1A2, Canada
    Clin Chest Med 30:701-16, viii. 2009
    ..This article reviews the existing evidence base on TB diagnostics, describes the progress of new technologies, and ends with a review of cost-effectiveness and modeling studies on the potential effect of new diagnostics in TB control...
  12. ncbi Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories
    P C Y Woo
    State Key Laboratory of Emerging Infectious Diseases and Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China
    Clin Microbiol Infect 14:908-34. 2008
    ....
  13. pmc Human coinfection with Bartonella henselae and two hemotropic mycoplasma variants resembling Mycoplasma ovis
    Jane E Sykes
    Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USA
    J Clin Microbiol 48:3782-5. 2010
    ..Coinfection with similar variants has been described in sheep. This represents the first report of human infection with this organism. The veterinarian was coinfected with Bartonella henselae...
  14. ncbi Comparison of four culture systems for Mycobacterium tuberculosis in the Zambian National Reference Laboratory
    M Muyoyeta
    Zambia AIDS Related Tuberculosis Project ZAMBART, University of Zambia School of Medicine, Lusaka, Zambia
    Int J Tuberc Lung Dis 13:460-5. 2009
    ..National TB Reference Laboratory, Zambia...
  15. ncbi A method for growing a biofilm under low shear at the air-liquid interface using the drip flow biofilm reactor
    Darla M Goeres
    Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA
    Nat Protoc 4:783-8. 2009
    ..The entire procedure takes 13 h of active time that is distributed over 5 d...
  16. pmc Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification
    SIYI CHEN
    Department of Food Science, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA
    Appl Environ Microbiol 77:4008-16. 2011
    ..In conclusion, this rapid, accurate, and simple method to detect and quantify viable Salmonella cells in produce may present a useful tool for the produce industry to better control potential microbial hazards in produce...
  17. pmc Evaluation of the Abbott RealTime CT/NG assay in comparison to the Roche Cobas Amplicor CT/NG assay
    Annie Cheng
    Department of Pathology, Beth Israel Deaconess Medical Center, 330 Brookline Ave, YA309, Boston, MA 02215, USA
    J Clin Microbiol 49:1294-300. 2011
    ..gonorrhoeae and small amounts of C. trachomatis organisms. In summary, we conclude that the Abbott RealTime CT/NG assay is an accurate and automated new addition to the available testing options for C. trachomatis and N. gonorrhoeae...
  18. pmc Role of universal 16S rRNA gene PCR and sequencing in diagnosis of prosthetic joint infection
    M Marin
    Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Maranon, Madrid, Spain
    J Clin Microbiol 50:583-9. 2012
    ..A positive 16SPCR result is largely suggestive of PJI, even when few samples are analyzed; however, culture is generally more sensitive...
  19. pmc Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens
    Mirjam Kaestli
    Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia
    J Clin Microbiol 50:2059-62. 2012
    ....
  20. ncbi Evaluation of rapid mecA gene detection versus standard culture in staphylococcal chronic prosthetic joint infections
    Marie Titécat
    Institute of Microbiology, University Hospital Center, Lille, France
    Diagn Microbiol Infect Dis 73:318-21. 2012
    ..In conclusion, this technique would allow the implementation of first-line antibiotic treatment adapted to the presence of MRS or not within approximately 1 h and would strongly reduce the use of broad-spectrum antibiotics...
  21. pmc Performance of the GeneXpert CT/NG assay compared to that of the Aptima AC2 assay for detection of rectal Chlamydia trachomatis and Neisseria gonorrhoeae by use of residual Aptima Samples
    S D Goldenberg
    Centre for Clinical Infection and Diagnostics Research, Guy s and St Thomas NHS Foundation Trust and King s College, London, United Kingdom
    J Clin Microbiol 50:3867-9. 2012
    ..trachomatis and N. gonorrhoeae were 86%, 99.2%, 92.5%, and 98.4% and 91.1%, 100%, 100%, and 98.6%, respectively. Despite significant dilution of samples prior to GeneXpert testing, the assay performed well with excellent specificity...
  22. pmc Haemophilus haemolyticus isolates causing clinical disease
    Raydel Anderson
    Meningitis and Vaccine Preventable Disease Branch, Division of Bacterial Diseases, National Center for Infectious and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 50:2462-5. 2012
    ..All cases had different symptoms and presentations. Our study suggests that a testing scheme that includes reliable PCR assays and standard microbiological methods should be used in order to improve H. haemolyticus identification...
  23. pmc Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid
    Fernando Pasteran
    Servicio Antimicrobianos, Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas ANLIS Dr Carlos G Malbran, Ministerio de Salud y Ambiente, Ciudad Autonoma de Buenos Aires, Argentina
    J Clin Microbiol 48:1323-32. 2010
    ..These methods will provide the fast and useful information needed for targeting of antimicrobial therapy and appropriate infection control...
  24. pmc Highly sensitive direct detection and quantification of Burkholderia pseudomallei bacteria in environmental soil samples by using real-time PCR
    Trinh Thanh Trung
    Friedrich Loeffler Institute of Medical Microbiology, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
    Appl Environ Microbiol 77:6486-94. 2011
    ..Five samples were negative by both PCR methods and culture. In conclusion, our PCR-based system provides a highly specific and sensitive tool for the quantitative environmental surveillance of B. pseudomallei...
  25. pmc Acceleration of the direct identification of Staphylococcus aureus versus coagulase-negative staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods
    A J M Loonen
    PAMM Laboratory for Medical Microbiology, Eindhoven Veldhoven, The Netherlands
    Eur J Clin Microbiol Infect Dis 30:337-42. 2011
    ..An initial S. aureus or CNS load of 1 CFU/ml blood can be detected after 5 h of incubation in BacT/ALERT 3D by combining the sensitive isolation method and the tuf LightCycler assay...
  26. pmc Rapid, simple, and sensitive detection of Anaplasma phagocytophilum by loop-mediated isothermal amplification of the msp2 gene
    Lei Pan
    Department of Rickettsiology, National Institute of Communicable Disease Control and Prevention, China CDC, Changping, Beijing 102206, People s Republic of China
    J Clin Microbiol 49:4117-20. 2011
    ..phagocytophilum. This LAMP assay is a valuable method for rapid, cost-effective, and simple detection of A. phagocytophilum in the rural areas of China...
  27. pmc Adjunctive tests for diagnosis of tuberculosis: serology, ELISPOT for site-specific lymphocytes, urinary lipoarabinomannan, string test, and fine needle aspiration
    Jacqueline M Achkar
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Infect Dis 204:S1130-41. 2011
    ....
  28. ncbi Marine methylotrophs revealed by stable-isotope probing, multiple displacement amplification and metagenomics
    Josh D Neufeld
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    Environ Microbiol 10:1526-35. 2008
    ....
  29. pmc Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method
    Raquel Moure
    Department of Microbiology, IDIBELL Bellvitge Hospital, C Feixa Llarga s n 08907 Hospitalet de Llobregat, Barcelona, Spain
    J Clin Microbiol 49:1137-9. 2011
    ..GX offers high potential for the diagnosis of tuberculosis due to its capacity for direct detection of MTC, its rapidity, and its simplicity...
  30. pmc Differential mucoid exopolysaccharide production by members of the Burkholderia cepacia complex
    James E A Zlosnik
    Room 377, Child and Family Research Institute, 950 West 28th Ave, Vancouver, BC V5Z 4H4, Canada
    J Clin Microbiol 46:1470-3. 2008
    ..cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can convert from mucoid to nonmucoid...
  31. pmc Xpert MTB/RIF for rapid diagnosis of tuberculous lymphadenitis from fine-needle-aspiration biopsy specimens
    Louis J Ligthelm
    Division of Anatomical Pathology, National Health Laboratory Service, Tygerberg Hospital, Tygerberg, Cape Town, South Africa
    J Clin Microbiol 49:3967-70. 2011
    ..7% (95% confidence interval [CI], 86.6 to 100%) and 88.9% (95% CI, 69.6 to 100%), respectively, and it correctly identified 6/6 (100%) of the cytology smear-negative/culture-positive cases and 1 of 2 (50%) rifampin-resistant cases...
  32. pmc Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Laura Ferreira
    Unidad de Investigacion, Hospital Universitario de Salamanca, Salamanca, Spain
    J Clin Microbiol 48:2110-5. 2010
    ..2%). Our results show that MALDI-TOF MS allows bacterial identification directly from infected urine in a short time, with high accuracy, and especially when Gram-negative bacteria with high bacterial counts are involved...
  33. ncbi Economic analysis of the diagnosis of smear-negative pulmonary tuberculosis in South Africa: incorporation of a new rapid test, FASTPlaqueTB, into the diagnostic algorithm
    H Albert
    Biotec Laboratories Ltd, c o National Health Laboratory Service, Cape Town, Western Cape, South Africa
    Int J Tuberc Lung Dis 8:240-7. 2004
    ....
  34. ncbi Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology
    R Amann
    Max Planck Institut für Marine Mikrobiologie, Bremen, Germany
    FEMS Microbiol Rev 24:555-65. 2000
    ..In order to speed up the transformation of microbial ecology from a mostly descriptive to a hypothesis-driven, experimental science more intense use must be made of the taxonomic precision and quantitativeness of rRNA-targeted probes...
  35. ncbi Comparison of PCR-ELISA and LightCycler real-time PCR assays for detecting Salmonella spp. in milk and meat samples
    Sylvie Perelle
    Agence Française de Sécurité Sanitaire des Aliments AFSSA, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et sur les Procédés Agroalimentaires LERQAP, 1 5 rue de Belfort, 94700 Maisons Alfort, France
    Mol Cell Probes 18:409-20. 2004
    ..The results on naturally contaminated samples demonstrated that despite certain inhibition problems, LC-PCR and PCR-ELISA assays were highly specific and sensitive, and provide a powerful tool for detection of Salmonella in food samples...
  36. ncbi Scanty AFB smears: what's in a name?
    A Van Deun
    Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
    Int J Tuberc Lung Dis 8:816-23. 2004
    ..A tuberculosis control project in Bangladesh...
  37. pmc Searching for the elusive typhoid diagnostic
    Stephen Baker
    Oxford University Clinical Research Unit, The Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam
    BMC Infect Dis 10:45. 2010
    ..Here we discuss the current position of typhoid diagnostics, highlight the need for technological improvements and suggest potential ways of advancing this area...
  38. pmc Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria
    Kathryn E R Davis
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
    Appl Environ Microbiol 71:826-34. 2005
    ....
  39. pmc Rapid classification and identification of salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Ralf Dieckmann
    Federal Institute for Risk Assessment, National Salmonella Reference Laboratory, Diedersdorfer Weg 1, D 12277 Berlin, Germany
    Appl Environ Microbiol 74:7767-78. 2008
    ..The resulting mass spectrometry-based bacterial classification was very comparable to the results of DNA sequence-based methods. A rapid protocol that allowed identification of Salmonella subspecies in minutes was established...
  40. pmc Comparison of recoveries of mycobacterium tuberculosis using the automated BACTEC MGIT 960 system, the BACTEC 460 TB system, and Löwenstein-Jensen medium
    A Somoskovi
    Department of Respiratory Medicine, Semmelweis University, H 1536 Budapest 114, Hungary
    J Clin Microbiol 38:2395-7. 2000
    ..In conclusion, the nonradiometric, fully automated 7-ml BACTEC MGIT 960 system can be considered a viable alternative to the semiautomated, radiometric BACTEC 460 TB system...
  41. pmc PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients
    Theodore Spilker
    Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, 48109 Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium
    J Clin Microbiol 42:2074-9. 2004
    ..Thus, we have designed two PCR assays: one is specific for the genus Pseudomonas, while the other is specific for P. aeruginosa. Both assays show 100% sensitivity and specificity...
  42. ncbi Detection of microorganisms in blood specimens using matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a review
    M Drancourt
    UMR CNRS, IRD, IFR, Universite de la Mediterranee, Marseille, France
    Clin Microbiol Infect 16:1620-5. 2010
    ....
  43. pmc Rapid and simple approach for identification of Mycobacterium tuberculosis complex isolates by PCR-based genomic deletion analysis
    Linda M Parsons
    Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
    J Clin Microbiol 40:2339-45. 2002
    ..bovis and M. tuberculosis, when it is important to distinguish between zoonotic sources (i.e., cattle and unpasteurized dairy products) and human sources of tuberculosis disease...
  44. ncbi Diagnosis of Mycobacterium ulcerans infection (Buruli ulcer) at a treatment centre in Ghana: a retrospective analysis of laboratory results of clinically diagnosed cases
    Ernestina Mensah-Quainoo
    Ghana Health Service, Ministry of Health, Tema, Ghana
    Trop Med Int Health 13:191-8. 2008
    ..However, being retrospective, this study did not permit sensitivity and negative predictive value analysis...
  45. pmc Quantification of bias related to the extraction of DNA directly from soils
    A Frostegard
    Laboratoire d Ecologie Microbienne du Sol, UMR CNRS 5557, Universite Claude Bernard Lyon 1, F 69622 Villeurbanne Cedex, France
    Appl Environ Microbiol 65:5409-20. 1999
    ..We also investigated different purification techniques and optimized the PCR methods in order to develop a protocol based on hybridization of the PCR products and quantification by phosphorimaging...
  46. pmc Meta-analysis of BACTEC MGIT 960 and BACTEC 460 TB, with or without solid media, for detection of mycobacteria
    M Cruciani
    HIV Outpatient Clinic, Center of Preventive Medicine, Verona, Italy
    J Clin Microbiol 42:2321-5. 2004
    ..5 and 99.6% and 85.8 and 99.9%, respectively. Combined with solid media, the sensitivity of the two systems increased to 87.7 and 89.7%, respectively...
  47. pmc Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting
    Martin Christner
    Department of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Martinistr 52, 20246 Hamburg, Germany
    J Clin Microbiol 48:1584-91. 2010
    ..With turnaround times around 100 min, the approach allowed for reliable pathogen identification at the day of blood culture positivity, providing treatment-relevant information within the critical phase of septic illness...
  48. ncbi Point-of-care Xpert® MTB/RIF for smear-negative tuberculosis suspects at a primary care clinic in South Africa
    A van Rie
    Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Int J Tuberc Lung Dis 17:368-72. 2013
    ..To assess the clinical utility and cost of point-of-care Xpert® MTB/RIF for the diagnosis of smear-negative tuberculosis (TB)...
  49. ncbi Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    L Ferreira
    Unidad de Investigacion, Hospital Universitario de Salamanca, Salamanca, Spain
    Clin Microbiol Infect 17:546-51. 2011
    ....
  50. pmc Sputum monitoring during tuberculosis treatment for predicting outcome: systematic review and meta-analysis
    David J Horne
    Division of Pulmonary and Critical Care Medicine, Seattle, WA 98104, USA
    Lancet Infect Dis 10:387-94. 2010
    ..Although we pooled a diverse group of patients, the individual studies had similar performance characteristics. Better predictive markers are needed...
  51. ncbi Optimisation of storage conditions for maintaining culturability of penicillin-susceptible and penicillin-resistant isolates of Streptococcus pneumoniae in transport medium
    C K Mason
    Oral Research Centre, School of Dentistry, Queen s University, Belfast, Northern Ireland, UK
    Br J Biomed Sci 67:1-4. 2010
    ..This work will help to establish robust sampling protocols when performing community studies to ensure culturability of comparison between community and laboratory pneumococci survival...
  52. pmc Haemophilus influenzae outer membrane protein P6 molecular characterization may not differentiate all strains of H. Influenzae from H. haemolyticus
    Arthur Chang
    Rochester General Hospital, Rochester General Research Institute, Center for Infectious Diseases and Immunology, Rochester, NY 14621, USA
    J Clin Microbiol 48:3756-7. 2010
    ..Further testing using RapID NH and multilocus sequence analysis may not help identify some isolates. Translated OMP P6 gene sequences are not conserved among all isolates presumed to be H. influenzae...
  53. pmc Novel chemically modified liquid medium that will support the growth of seven bartonella species
    Ricardo G Maggi
    Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    J Clin Microbiol 43:2651-5. 2005
    ..This liquid growth medium may provide an advantage over conventional direct blood agar plating for the diagnostic confirmation of bartonellosis...
  54. pmc Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR
    Vincent Cattoir
    Laboratoire de bactériologie virologie hygiène, Hopital Henri Mondor, Assistance Publique Hopitaux de Paris, Creteil, France
    Ann Clin Microbiol Antimicrob 9:21. 2010
    ..The present study was aimed to develop a novel quantitative PCR (qPCR) assay, using ecfX as the specific target gene, for the rapid and accurate identification of P. aeruginosa from positive blood cultures (BCs)...
  55. ncbi Laboratory diagnosis of Buruli ulcer disease
    Marcus Beissner
    Department of Infectious Diseases and Tropical Medicine, Ludwig Maximilians University Munich, Leopoldstrasse 5, 80802 Munich, Germany
    Future Microbiol 5:363-70. 2010
    ..Currently, cultures are considered the only valid confirmatory test for the detection of viable bacilli...
  56. ncbi Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens
    Catriona Logan
    Department of Microbiology, Our Lady s Children s Hospital, Crumlin, Dublin, Ireland
    Diagn Microbiol Infect Dis 68:358-65. 2010
    ..aeruginosa culture results...
  57. pmc Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets
    Guy Prod'hom
    Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland
    J Clin Microbiol 48:1481-3. 2010
    ..Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising...
  58. pmc Evaluation of tuberculosis diagnostics: establishing an evidence base around the public health impact
    Richard J Lessells
    Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, UK
    J Infect Dis 204:S1187-95. 2011
    ..The urgent need for new diagnostics should not distract from pursuing rigorous scientific evaluation focused on public health impact...
  59. ncbi Rapid discrimination of environmental Vibrio by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    Rkia Eddabra
    UPRES EA 4438, Physiopathologie et Médecine Translationnelle, Laboratoire de Bactériologie de la Faculté de Médecine, Universite de Strasbourg, Hopitaux Universitaires de Strasbourg, 3 Rue Koeberle, 67000 Strasbourg, France
    Microbiol Res 167:226-30. 2012
    ..The results of MALDI TOF analysis show a high congruence of strain grouping demonstrating the accuracy and reliability of MALDI-TOF MS...
  60. ncbi Yield of acid-fast smear and mycobacterial culture for tuberculosis diagnosis in people with human immunodeficiency virus
    Patama Monkongdee
    Thailand Ministry of Public Health U S CDC Collaboration, Nonthaburi, Thailand
    Am J Respir Crit Care Med 180:903-8. 2009
    ..The World Health Organization recently revised its recommendations for tuberculosis (TB) diagnosis in people with HIV. Most studies cited to support these policies involved HIV-uninfected patients and only evaluated sputum specimens...
  61. ncbi Selective detection of live bacteria combining propidium monoazide sample treatment with microarray technology
    Andreas Nocker
    Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA
    J Microbiol Methods 76:253-61. 2009
    ..Minimization of detection signals deriving from dead cells will render data more relevant in studies including pathogen risk assessment...
  62. pmc Evaluation of a PCR test for detection of treponema pallidum in swabs and blood
    P A Grange
    Laboratoire de Recherche en Dermatologie, EA 1833, Centre National de Référence Syphilis, Faculte de Medecine, Universite Paris Descartes, Paris, France
    J Clin Microbiol 50:546-52. 2012
    ..pallidum detection in any of the specimens tested. Swab specimens from mucosal or skin lesions seemed to be more useful than blood for the efficient detection of the T. pallidum genome and, thus, for the diagnosis of syphilis...
  63. pmc Detection of Mycobacterium tuberculosis in blood by use of the Xpert MTB/RIF assay
    Padmapriya P Banada
    Center for Infectious Diseases, New Jersey Medical School UMDNJ, Newark, New Jersey, USA
    J Clin Microbiol 51:2317-22. 2013
    ..tuberculosis. This approach may be a useful method to detect extrapulmonary tuberculosis and the risk of death in immunocompromised patients...
  64. pmc Development of a new, combined rapid method using phage and PCR for detection and identification of viable Mycobacterium paratuberculosis bacteria within 48 hours
    Emma C Stanley
    Division of Food Sciences, School of Biosciences, Sutton Bonington Campus, University of Nottingham, Sutton Bonington LE12 5RD, United Kingdom
    Appl Environ Microbiol 73:1851-7. 2007
    ..Combining the plaque PCR technique with the phage-based detection assay allowed the rapid and specific detection of viable M. avium subsp. paratuberculosis in milk samples in just 48 h...
  65. pmc Neurotoxin gene profiling of clostridium botulinum types C and D native to different countries within Europe
    Cedric Woudstra
    ANSES French Agency for Food, Environmental and Occupational Health Safety, Food Safety Laboratory, Maisons Alfort, France
    Appl Environ Microbiol 78:3120-7. 2012
    ..Further investigations should now be performed in order to check that mosaic types dominate in Europe and that acquisition of mosaic types helps in survival or adaptation to particular niche...
  66. ncbi Comparison of BD GeneOhm real-time polymerase chain reaction with chromogenic and conventional culture methods for detection of group B Streptococcus in clinical samples
    Dawn Smith
    Microbiology Department, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK
    Diagn Microbiol Infect Dis 61:369-72. 2008
    ..5% versus 57% respectively, P < 0.02). After selective enrichment, 92.8% of GBS were isolated on chromID Strepto B compared with 89.2% isolated on sheep blood agar...
  67. pmc Microscopic-observation drug-susceptibility assay for the diagnosis of TB
    David A J Moore
    Department of Infectious Diseases and Immunity and the Wellcome Centre for Clinical Tropical Medicine, Imperial College London, Faculty of Medicine Hammersmith Campus, London, United Kingdom
    N Engl J Med 355:1539-50. 2006
    ....
  68. pmc An integrated approach to rapid diagnosis of tuberculosis and multidrug resistance using liquid culture and molecular methods in Russia
    Yanina Balabanova
    National Mycobacterium Reference Laboratory, Institute of Cell and Molecular Sciences, Queen Mary College, Barts and the London School of Medicine, University of London, London, United Kingdom
    PLoS ONE 4:e7129. 2009
    ..To analyse the feasibility, cost and performance of rapid tuberculosis (TB) molecular and culture systems, in a high multidrug-resistant TB (MDR TB) middle-income region (Samara, Russia) and provide evidence for WHO policy change...
  69. pmc Evaluation of a rapid, real-time intrapartum group B streptococcus assay
    Brett C Young
    Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Am J Obstet Gynecol 205:372.e1-6. 2011
    ..We sought to evaluate an intrapartum nucleic acid amplification test (NAAT) for group B streptococcus (GBS)...
  70. ncbi Rapid identification of Legionella spp. by MALDI-TOF MS based protein mass fingerprinting
    Valeria Gaia
    Cantonal Institute of Microbiology, Bellinzona, Switzerland
    Syst Appl Microbiol 34:40-4. 2011
    ..The identification of Legionella spp. by MALDI-TOF MS is rapid, easy to perform and has the advantage of being time- and cost-saving, in comparison to sequence-based identification...
  71. ncbi Diagnosis of acute leptospirosis
    Takao Toyokawa
    Field Epidemiology Training Program Japan, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, Japan
    Expert Rev Anti Infect Ther 9:111-21. 2011
    ..will accelerate our understanding of Leptospira pathogenesis and provide insights into the development of more efficient and accurate diagnostic tests for acute-phase leptospirosis...
  72. pmc Comparison of premier CAMPY enzyme immunoassay (EIA), ProSpecT Campylobacter EIA, and ImmunoCard STAT! CAMPY tests with culture for laboratory diagnosis of Campylobacter enteric infections
    Paul A Granato
    Department of Microbiology and Immunology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    J Clin Microbiol 48:4022-7. 2010
    ..jejuni and C. coli and that conventional culture may no longer be recognized as the "gold standard" for diagnosis...
  73. pmc Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs
    Lance R Peterson
    NorthShore University Healthsystem, Department of Pathology and Laboratory Medicine, 2650 Ridge Avenue, Walgreen Bldg, SB525, Evanston, IL 60201, USA
    J Clin Microbiol 48:1661-6. 2010
    ..001) with the LightCycler MRSA advanced test. The sensitivity of direct culture was 80.4%. The LightCycler MRSA advanced test is a useful tool for sensitive and rapid detection of MRSA nasal colonization...
  74. pmc Contribution of amoebic coculture to recovery of Legionella isolates from respiratory samples: prospective analysis over a period of 32 months
    G Descours
    Universite de Lyon, Lyon, France
    J Clin Microbiol 50:1725-6. 2012
    ..1%. Amoebic coculture was particularly efficient in isolating Legionella spp. from respiratory samples contaminated with oropharyngeal flora...
  75. pmc Maximizing capture efficiency and specificity of magnetic separation for Mycobacterium avium subsp. paratuberculosis cells
    Antonio Foddai
    Institute of Agri food and Land Use, School of Biological Sciences, Queen s University Belfast, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland
    Appl Environ Microbiol 76:7550-8. 2010
    ..3 PFU/ml). This PMS-phage assay represents a novel, rapid method for the detection and enumeration of viable M. avium subsp. paratuberculosis organisms in milk, and potentially other sample matrices, with results available within 48 h...
  76. ncbi Comparison of MALDI TOF with conventional identification of clinically relevant bacteria
    Martin Risch
    Labormedizinisches zentrum Dr Risch, Liebefeld, Switzerland
    Swiss Med Wkly 140:w13095. 2010
    ..Comparison between results of the new procedure with those obtained by conventional testing is mandatory...
  77. pmc Identification of HACEK clinical isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Marc Roger Couturier
    University of Utah School of Medicine, Department of Pathology, Salt Lake City, UT 84108, USA
    J Clin Microbiol 49:1104-6. 2011
    ..Ninety-three percent of HACEK organisms were identified correctly to the genus level using the Bruker database, and 100% were identified to the genus level using a custom database that included clinical isolates...
  78. pmc Direct amplification of rRNA genes in diagnosis of bacterial infections
    K Rantakokko-Jalava
    Department of Medical Microbiology, University of Turku, Turku, Finland
    J Clin Microbiol 38:32-9. 2000
    ....
  79. pmc Clinical value of Treponema pallidum real-time PCR for diagnosis of syphilis
    R Heymans
    Public Health Laboratory, Amsterdam Public Health Service, Amsterdam, The Netherlands
    J Clin Microbiol 48:497-502. 2010
    ....
  80. ncbi A new multiplex PCR for differential identification of Shigella flexneri and Shigella sonnei and detection of Shigella virulence determinants
    M J Farfán
    Departamento de Pediatria, Hospital Dr Luis Calvo Mackenna, Facultad de Medicina, Universidad de Chile, Santiago, Chile
    Epidemiol Infect 138:525-33. 2010
    ..flexneri and S. sonnei. The technique was sensitive, reproducible, specific and simple to perform, providing a new tool with the potential to be employed for epidemiological and diagnostic purposes...
  81. pmc Laboratory practices and incidence of non-O157 shiga toxin-producing Escherichia coli infections
    Kathleen A Stigi
    Washington State Department of Health, Shoreline, WA 98155, USA
    Emerg Infect Dis 18:477-9. 2012
    ..Despite increased assay use, only half of processed stool specimens underwent Shiga toxin testing during 2010, suggesting substantial underdetection of non-O157 STEC infections...
  82. ncbi Performance of a rapid antigen-detection test and throat culture in community pediatric offices: implications for management of pharyngitis
    Robert R Tanz
    Department of Pediatrics, Children s Memorial Hospital and Northwestern University Feinberg School of Medicine, Chicago, IL 60614, USA
    Pediatrics 123:437-44. 2009
    ....
  83. pmc Rapid genus- and species-specific identification of Cronobacter spp. by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Roger Stephan
    Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstr 272, CH 8057 Zurich, Switzerland
    J Clin Microbiol 48:2846-51. 2010
    ..Our study demonstrates that MALDI-TOF MS is a reliable and powerful tool for the rapid identification of Cronobacter strains to the genus and species level...
  84. ncbi Costs and cost-effectiveness of tuberculosis cultures using solid and liquid media in a developing country
    D H Mueller
    Health Economics and Financing Programme, Department of Public Health and Policy, London School of Hygiene and Tropical Medicine, London, UK
    Int J Tuberc Lung Dis 12:1196-202. 2008
    ..The expansion of culture has been proposed to aid tuberculosis (TB) control in developing countries...
  85. pmc Three-hour molecular detection of Campylobacter, Salmonella, Yersinia, and Shigella species in feces with accuracy as high as that of culture
    Scott A Cunningham
    Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First St S W, Rochester, MN 55905, USA
    J Clin Microbiol 48:2929-33. 2010
    ..Paired with reflexive culture of stools testing positive, this should provide an improvement in care for patients with acute infectious diarrheal disease...
  86. pmc Does bleach processing increase the accuracy of sputum smear microscopy for diagnosing pulmonary tuberculosis?
    A Cattamanchi
    Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital, University of California, San Francisco, CA 94110, USA
    J Clin Microbiol 48:2433-9. 2010
    ..Further research should focus on alternative approaches to optimizing smear microscopy, such as light-emitting diode fluorescence microscopy and same-day sputum collection strategies...
  87. ncbi Diagnosis of pulmonary tuberculosis in children: new advances
    Heather J Zar
    Department of Paediatrics and Child Health, Red Cross War Memorial Children s Hospital, University of Cape Town, South Africa
    Expert Rev Anti Infect Ther 8:277-88. 2010
    ..Greater efforts to obtain a microbiologic diagnosis should be made in children, even in primary care settings. Further research to develop a more accurate, cost-effective and simple diagnostic test for childhood TB is urgently needed...
  88. pmc PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis
    Tomer Avni
    Department of Medicine E, Rabin Medical Center, Belinson Hospital, Ramat Aviv, Israel
    J Clin Microbiol 48:489-96. 2010
    ..Currently available methods for PCR with blood samples for the diagnosis of IPD lack the sensitivity and specificity necessary for clinical practice...
  89. ncbi Accuracy of identification and susceptibility results by direct inoculation of Vitek 2 cards from positive BACTEC cultures
    J J Kerremans
    Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre Rotterdam, The Netherlands
    Eur J Clin Microbiol Infect Dis 23:892-8. 2004
    ..Susceptibility results for this antibiotic, if obtained using direct inoculation, should not be reported to the clinician...
  90. pmc Molecular analysis improves pathogen identification and epidemiologic study of pediatric parapneumonic empyema
    Anne J Blaschke
    Department of Pediatrics, University of Utah, Salt, Lake City, UT, USA
    Pediatr Infect Dis J 30:289-94. 2011
    ..Epidemiologic study is complicated by the low frequency of positive cultures. We sought to describe the epidemiology of PPE in children using molecular analysis of pleural fluid...
  91. ncbi Usefulness of real-time PCR for lytA, ply, and Spn9802 on plasma samples for the diagnosis of pneumococcal pneumonia
    G Abdeldaim
    Department of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden
    Clin Microbiol Infect 16:1135-41. 2010
    ....
  92. pmc Investigation of biofilm formation in clinical isolates of Staphylococcus aureus
    James E Cassat
    Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR, USA
    Methods Mol Biol 391:127-44. 2007
    ..However, because this system is not generally available, we restrict technical comments in this chapter to our use of an implanted catheter model evaluated by direct microbio-logical analysis of explanted catheters (2)...
  93. pmc Burkholderia pseudomallei misidentified by automated system
    Christoph Weissert
    Institute for Clinical Microbiology and Immunology, St Gallen CH 9001, Switzerland
    Emerg Infect Dis 15:1799-801. 2009
    ..The organism was eventually identified by molecular methods as B. pseudomallei...
  94. ncbi Broad diversity of viable bacteria in 'sterile' (0.2 microm) filtered water
    Martin W Hahn
    Institute for Limnology, Austrian Academy of Sciences, Mondseestrasse 9, 5310 Mondsee, Austria
    Res Microbiol 155:688-91. 2004
    ..2-microm filters, and it is concluded that microbiologists may have dramatically underestimated the diversity of 0.2 microm-filterable bacteria...
  95. pmc Evaluation of BACTEC Mycobacteria Growth Indicator Tube (MGIT 960) automated system for drug susceptibility testing of Mycobacterium tuberculosis
    F Ardito
    Istituto di Microbiologia, , Rome, Italy
    J Clin Microbiol 39:4440-4. 2001
    ..05). In conclusion, the performance of BACTEC MGIT 960 was found similar to that of BACTEC 460 TB and this new system can be considered a good alternative to the radiometric method for routine susceptibility testing of M. tuberculosis...
  96. ncbi Interactions and self-organization in the soil-microbe complex
    I M Young
    Scottish Informatics, Mathematics, Biology, and Statistics SIMBIOS Centre, University of Abertay, Bell Street, Dundee, DD1 1HG Scotland, UK
    Science 304:1634-7. 2004
    ..We introduce the concept that the soil-microbe system is self-organized and suggest new priorities for research based on an integrative approach that combines biochemistry and biophysics...
  97. ncbi Comparison of rapid intrapartum screening methods for group B streptococcal vaginal colonization
    Natali Aziz
    Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA 94025, USA
    J Matern Fetal Neonatal Med 18:225-9. 2005
    ..To compare optical immunoassay (OIA) and rapid polymerase-chain reaction (PCR) with enrichment broth culture for intrapartum detection of vaginal group B streptococcal (GBS) colonization...
  98. pmc Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays
    D M Wolk
    University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 47:823-6. 2009
    ..aureus. Sensitivity was 97.1% and 98.3% for MRSA in wound and blood culture specimens, respectively. Sensitivity was 100% for S. aureus from both specimen types...
  99. ncbi Improved reliability of Pseudomonas aeruginosa PCR detection by the use of the species-specific ecfX gene target
    R Lavenir
    Research group on Bacterial Opportunistic Pathogens and Environment Université de Lyon, Lyon, F 69003, France
    J Microbiol Methods 70:20-9. 2007
    ..aeruginosa. These investigations suggest a preferential colonization of water rather than soil environments by P. aeruginosa. Detection limits of P. aeruginosa in environmental samples were improved by the ecfX PCR screening...
  100. pmc Optimization of media for detection of hydrogen peroxide production by Lactobacillus species
    L K Rabe
    Magee Women s Research Institute, The University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Clin Microbiol 41:3260-4. 2003
    ..This new medium better supports the growth of a wider range of Lactobacillus strains isolated from the vagina and enhances the color production of H(2)O(2)-producing strains...
  101. pmc Cultivation-dependent and -independent approaches for determining bacterial diversity in heavy-metal-contaminated soil
    Richard J Ellis
    NERC Centre for Population Biology, Imperial College London, Ascot, Berkshire SL5 7PY, United Kingdom
    Appl Environ Microbiol 69:3223-30. 2003
    ..Thus, it appears that plate counts may be a more appropriate method for determining the effect of heavy metals on soil bacteria than culture-independent approaches...

Research Grants42

  1. POPULATION BASED INVESTIGATIONS OF TUBERCULOSIS
    David Alland; Fiscal Year: 2004
    ..Studies of these sequence variations in clinical strains will be used to determine the associations between specific mutations and the phenotypes of infectivity and virulence. ..
  2. The Role of Peroxiredoxins in Immunity and Aging
    SVETLANA RADYUK; Fiscal Year: 2006
    ..peptides and sensitivity of Drosophila to infection will be determined using molecular, biological, and bacteriological techniques. In addition, regulation of the immune system by peroxiredoxins will be assessed as a function of age...
  3. Neurodevelopment and HIV/AIDS
    Annelies Van Rie; Fiscal Year: 2005
    ....
  4. Pediatric HIV-encephalopathy in DRC: effect of ART & role of compartmentalization
    Annelies Van Rie; Fiscal Year: 2010
    ....
  5. Sample Processing Cartridges for Rapid PCR TB Detection
    David Alland; Fiscal Year: 2008
    ..The system will then be retested using a large number of clinical sputum specimens. Finally, we will develop complete documentation on the assay including production, and development reports. [unreadable] [unreadable] [unreadable]..
  6. TUBERCULOSIS GENOTYPING AND EVOLUTIONARY CONSORTIUM
    David Alland; Fiscal Year: 2005
    ..4) To identify and validate an informative set of SNPs for worldwide evolutionary and epidemiological analyses. ..
  7. Towards drugs that prevent resistance to the HIV OI, TB
    David Alland; Fiscal Year: 2006
    ..if embB306 mutations affect biosynthesis of LAM, capped LAM, arabinogalactan (AG) or other cell wall components in the presence and absence of EMB. ..
  8. Detection of Select Agents in Single-Well Assays
    David Alland; Fiscal Year: 2007
    ..3. To develop PCR assays that utilize "color-triplet coding" to uniquely label as many as 56 different species-specific molecular beacons. ..
  9. The Basis of Anthrax-Induced Vascular Damage
    James Kirby; Fiscal Year: 2006
    ..As part of this exploration, we hope to identify and test new therapeutic strategies. [unreadable] [unreadable]..
  10. PCR assays to detect individual pneumococcal serotypes
    LORRY RUBIN; Fiscal Year: 2006
    ..Furthermore, they will be critical for determining the effects of current and future pneumococcal vaccines on serotype-specific carriage. ..
  11. CONGENITAL CMV INFECTION IN OFFSPRING OF IMMUNE MOTHERS
    Suresh Boppana; Fiscal Year: 2005
    ....
  12. Anti-larval measures for malaria control in The Gambia
    Steven Lindsay; Fiscal Year: 2007
    ..The results from this study will make a major contribution to quantifying the importance of the use of Bs and Bti in the fight against malaria. ..
  13. Innate Immunity and exacerbations of COPD
    Sanjay Sethi; Fiscal Year: 2009
    ..The overall goal of this research is to identify specific aspects of the innate defense of the lung that determine the frequency of exacerbations in COPD that will serve as potential therapeutic targets to reduce exacerbations. ..
  14. MENTORED PATIENT ORIENTED RESEARCH CAREER DEVELOPMENT AW
    Margaret Rosenfeld; Fiscal Year: 2004
    ..The results and experience gained by the proposed studies may aid in the rigorous conduct of future clinical trials of inhaled agents in infants both with and without CF. ..
  15. Analysis of Borrelia Burgdorferi Erp Proteins
    Brian Stevenson; Fiscal Year: 2010
    ..Results of our studies will provide important insight into the mechanisms by which B. burgdorferi infects humans. ..
  16. HAEMOPHILUS ANTIGEN REGULATION OF INFLAMMATION IN COPD
    Sanjay Sethi; Fiscal Year: 2003
    ..Identification of the integral bacterial and host components that contribute to immune-mediated inflammation in COPD will ultimately lead to novel therapeutic strategies to alter disease progression and improve outcome in COPD. ..
  17. Beta-defensins and pulmonary host defense
    Timothy Starner; Fiscal Year: 2006
    ..This environment will maximize the potential for the principal investigator to establish himself in a basic science career as an academic independent investigator. ..
  18. Environmental Regulation of Staphylococcus epidermidis PIA Synthesis
    GREG SOMERVILLE; Fiscal Year: 2007
    ..The work contained in this proposal is a first step toward inhibiting the formation of this complex sugar, preventing the formation of a bacterial biofilm, and providing physicians a way to treat patients. [unreadable] [unreadable]..
  19. Identifying targets for diagnosing oral candidiasis
    David Schofield; Fiscal Year: 2005
    ..Since Candida species are the most important fungal pathogen of the oral cavity, the rapid and accurate diagnosis of oral candidiasis using novel disease biomarkers will significantly improve oral health. ..
  20. Azithromycin in Control of Trachoma II
    Julius Schachter; Fiscal Year: 2005
    ..In sum, this study should provide a rational approach to use of community-wide azithromycin treatment to eliminate blinding trachoma as a public health problem. ..
  21. ITO IN DOGS WITH INHERITED VENTRICULAR ARRHYTHMIAS
    Bruce Kornreich; Fiscal Year: 2005
    ..abstract_text> ..
  22. Mobilization of Candida albicans Biofilms
    Peter Suci; Fiscal Year: 2007
    ..albicans biofilm communities, it will provide the basis for development of methods for screening for adjuvant agents that will inhibit dissemination of these fungal infections. [unreadable] [unreadable] [unreadable]..
  23. Wolbachia as an Agent for Population Replacement in Anopheles Gambiae
    Jason Rasgon; Fiscal Year: 2008
    ..We propose to evaluate transfer Wolbachia infection into Anopheles gambiae and evaluate the symbiont as a candidate transgene drive system for malaria control. [unreadable] [unreadable] [unreadable]..
  24. Appropriate Pneumococcal Vaccination in Infants in Fiji
    Fiona Russell; Fiscal Year: 2007
    ..The results of this study will form the basis of an effectiveness evaluation following the pilot introduction of pneumococcal immunization into Fiji in 3-5 years time. [unreadable] [unreadable]..
  25. Purine Analog Anti-Mycobacterial Drug Development
    William Parker; Fiscal Year: 2009
    ..tb activity;and (4) design and synthesis of purine and purine nucleoside analogs with selective activity against M. tb. ..
  26. HMG-1/Y Regulation of NOS2 Expression in Endotoxemia
    REBECCA BARON; Fiscal Year: 2007
    ..These experiments will allow us to determine the role of HMG-I/Y in regulating NOS2 expression during endotoxemia and may reveal a novel target for improving outcomes from sepsis. [unreadable] [unreadable] [unreadable]..
  27. UNDERSTANDING THE PATHOGENESIS OF STAPHYLOCOCCUS AUREUS
    Vance Fowler; Fiscal Year: 2003
    ..Accomplishing the aims of this proposal will contribute to improved clinical management of patients with S. aureus bacteremia, and will add to the understanding of S. aureus virulence factors. ..
  28. Biofilm growth and detachment of an oral pathogen
    Jeffrey Kaplan; Fiscal Year: 2007
    ..abstract_text> ..
  29. Borrelia burgdorferi LuxS-mediated quorum sensing
    Brian Stevenson; Fiscal Year: 2007
    ..burgdorferi and its hosts, as well as identifying potential bacterial targets for improved therapies to prevent and treat Lyme disease. ..
  30. DETECTING NEONATAL SEPSIS USING MOLECULAR TECHNIQUES
    JEANNE JORDAN; Fiscal Year: 2003
    ....
  31. Exposure Intervention in a Rural Pediatric Asthma Cohort
    Peter Thorne; Fiscal Year: 2005
    ..Detailed health and home hygiene questionnaires and home inspections will be conducted at baseline and one year later, as will child spirometry. ..
  32. URINE LOSS AND PROLAPSE IN NUNS AND THEIR PAROUS SISTERS
    GUNHILDE BUCHSBAUM; Fiscal Year: 2003
    ..A matched pair logistic regression will be performed to obtain an adjusted estimate of the impact of familiality and vaginal delivery in UT and POP, taking into account other risk factors. ..
  33. THE ROLE OF MYCOBACTERIA IN CROHN'S DISEASE
    David Graham; Fiscal Year: 2005
    ..The results of this study should either confirm or refute the proposed etiologic association of M. paratuberculosis and Crohn's disease as well as the identification of patients with Crohn's disease and M. paratuberculosis. ..
  34. Phospholipid-induced Motility in Bacillus cereus Group
    R Fall; Fiscal Year: 2004
    ..Such information will be thebasis for future studies or the biochemical mechanism of gliding motility in B. cereus family, and may lead to new strategies to control tissue invasion by these pathogens. ..
  35. Catheter Dwell Time and Risk of Bloodstream Infections in Hospitalized Neonates
    Aaron M Milstone; Fiscal Year: 2011
    ..This project will help to develop evidence-based strategies to prevent bloodstream infections among vulnerable infants hospitalized in the neonatal intensive care unit. ..
  36. DNA DAMAGE AND REPAIR IN SALMONELLA PATHOGENESIS
    Ferric Fang; Fiscal Year: 2007
    ..unreadable] [unreadable]..