- Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology
Department of Medicine, New Jersey Medical School, University of Medicine and Dentistry, New Jersey, Newark, New Jersey 07103, USA
J Clin Microbiol 48:229-37. 2010
..2%) susceptible cases. Specificity rose to 100% after correcting for a conventional susceptibility test error. In conclusion, this highly sensitive and simple-to-use system can detect M. tuberculosis directly from sputum in less than 2 h...
- Sonication of removed hip and knee prostheses for diagnosis of infection
Division of Infectious Diseases, Department of Internal Medicine, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
N Engl J Med 357:654-63. 2007
..We hypothesized that culturing of samples obtained from the prosthesis would improve the microbiologic diagnosis of prosthetic-joint infection...
- Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review
Karen R Steingart
Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital, University of California, San Francisco, CA, USA
Lancet Infect Dis 6:664-74. 2006
..Operational studies are needed to determine whether the increased sensitivity provided by processing methods is sufficient to offset their increased cost, complexity, and potential biohazards, and to examine their feasibility...
- Liquid vs. solid culture for tuberculosis: performance and cost in a resource-constrained setting
V N Chihota
Aurum Institute for Health Research, Johannesburg, South Africa
Int J Tuberc Lung Dis 14:1024-31. 2010
..National Health Laboratory Services tuberculosis (TB) laboratory, South Africa...
- Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Pôle des Maladies Infectieuses, Assistance Publique Hopitaux de Marseille, URMITE, Unite Mixte de Recherche, Centre National de la Recherche Scientifique Institut pour la Recherche et le Développement 6236, Universite de la Mediterranee, Marseille, France
Clin Infect Dis 49:543-51. 2009
..Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry accurately identifies both selected bacteria and bacteria in select clinical situations. It has not been evaluated for routine use in the clinic...
- Recommendations for diagnosis of shiga toxin--producing Escherichia coli infections by clinical laboratories
L Hannah Gould
Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, CDC, Atlanta, Georgia 30333, USA
MMWR Recomm Rep 58:1-14. 2009
..Improving the diagnostic accuracy of STEC infection by clinical laboratories should ensure prompt diagnosis and treatment of these infections in patients and increase detection of STEC outbreaks in the community...
- Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry
Bernard La Scola
Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille and URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
PLoS ONE 4:e8041. 2009
..In order to speed up the identification process, in the present work we attempted bacterial identification directly from blood culture bottles detected positive by the automate...
- Mass spectrometry tools for the classification and identification of bacteria
Max Planck Institute for Molecular Genetics, Otto Warburg Laboratory, Ihnestrasse 63 73, D 14195 Berlin, Germany
Nat Rev Microbiol 8:74-82. 2010
..In this Review we discuss the application range of these mass spectrometry procedures and their potential for successful transfer into microbiology laboratories...
- Diagnostic accuracy of a urine lipoarabinomannan test for tuberculosis in hospitalized patients in a High HIV prevalence setting
Department of Medicine, Johns Hopkins University Center for Tuberculosis Research, Baltimore, MD 21231, USA
J Acquir Immune Defic Syndr 52:145-51. 2009
..Effective tuberculosis (TB) control in HIV-prevalent settings is hindered by absence of accurate, rapid TB diagnostic tests. We evaluated the accuracy of a urine lipoarabinomannan (LAM) test for TB diagnosis in South Africa...
- Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry
Lindsay G Stevenson
Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892 1508, USA
J Clin Microbiol 48:444-7. 2010
..This method provides a rapid, accurate, definitive identification of bacteria within 1 h of detection in positive blood cultures with the caveat that the identification of S. pneumoniae would have to be confirmed by an alternative test...
- Novel and improved technologies for tuberculosis diagnosis: progress and challenges
Department of Epidemiology, Biostatistics and Occupational Health, McGill University, 1020 Pine Avenue West, Montreal, H3A 1A2, Canada
Clin Chest Med 30:701-16, viii. 2009
..This article reviews the existing evidence base on TB diagnostics, describes the progress of new technologies, and ends with a review of cost-effectiveness and modeling studies on the potential effect of new diagnostics in TB control...
- Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories
P C Y Woo
State Key Laboratory of Emerging Infectious Diseases and Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China
Clin Microbiol Infect 14:908-34. 2008
- Human coinfection with Bartonella henselae and two hemotropic mycoplasma variants resembling Mycoplasma ovis
Jane E Sykes
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USA
J Clin Microbiol 48:3782-5. 2010
..Coinfection with similar variants has been described in sheep. This represents the first report of human infection with this organism. The veterinarian was coinfected with Bartonella henselae...
- Comparison of four culture systems for Mycobacterium tuberculosis in the Zambian National Reference Laboratory
Zambia AIDS Related Tuberculosis Project ZAMBART, University of Zambia School of Medicine, Lusaka, Zambia
Int J Tuberc Lung Dis 13:460-5. 2009
..National TB Reference Laboratory, Zambia...
- A method for growing a biofilm under low shear at the air-liquid interface using the drip flow biofilm reactor
Darla M Goeres
Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA
Nat Protoc 4:783-8. 2009
..The entire procedure takes 13 h of active time that is distributed over 5 d...
- Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification
Department of Food Science, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA
Appl Environ Microbiol 77:4008-16. 2011
..In conclusion, this rapid, accurate, and simple method to detect and quantify viable Salmonella cells in produce may present a useful tool for the produce industry to better control potential microbial hazards in produce...
- Evaluation of the Abbott RealTime CT/NG assay in comparison to the Roche Cobas Amplicor CT/NG assay
Department of Pathology, Beth Israel Deaconess Medical Center, 330 Brookline Ave, YA309, Boston, MA 02215, USA
J Clin Microbiol 49:1294-300. 2011
..gonorrhoeae and small amounts of C. trachomatis organisms. In summary, we conclude that the Abbott RealTime CT/NG assay is an accurate and automated new addition to the available testing options for C. trachomatis and N. gonorrhoeae...
- Role of universal 16S rRNA gene PCR and sequencing in diagnosis of prosthetic joint infection
Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Maranon, Madrid, Spain
J Clin Microbiol 50:583-9. 2012
..A positive 16SPCR result is largely suggestive of PJI, even when few samples are analyzed; however, culture is generally more sensitive...
- Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens
Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia
J Clin Microbiol 50:2059-62. 2012
- Evaluation of rapid mecA gene detection versus standard culture in staphylococcal chronic prosthetic joint infections
Institute of Microbiology, University Hospital Center, Lille, France
Diagn Microbiol Infect Dis 73:318-21. 2012
..In conclusion, this technique would allow the implementation of first-line antibiotic treatment adapted to the presence of MRS or not within approximately 1 h and would strongly reduce the use of broad-spectrum antibiotics...
- Performance of the GeneXpert CT/NG assay compared to that of the Aptima AC2 assay for detection of rectal Chlamydia trachomatis and Neisseria gonorrhoeae by use of residual Aptima Samples
S D Goldenberg
Centre for Clinical Infection and Diagnostics Research, Guy s and St Thomas NHS Foundation Trust and King s College, London, United Kingdom
J Clin Microbiol 50:3867-9. 2012
..trachomatis and N. gonorrhoeae were 86%, 99.2%, 92.5%, and 98.4% and 91.1%, 100%, 100%, and 98.6%, respectively. Despite significant dilution of samples prior to GeneXpert testing, the assay performed well with excellent specificity...
- Haemophilus haemolyticus isolates causing clinical disease
Meningitis and Vaccine Preventable Disease Branch, Division of Bacterial Diseases, National Center for Infectious and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
J Clin Microbiol 50:2462-5. 2012
..All cases had different symptoms and presentations. Our study suggests that a testing scheme that includes reliable PCR assays and standard microbiological methods should be used in order to improve H. haemolyticus identification...
- Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid
Servicio Antimicrobianos, Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas ANLIS Dr Carlos G Malbran, Ministerio de Salud y Ambiente, Ciudad Autonoma de Buenos Aires, Argentina
J Clin Microbiol 48:1323-32. 2010
..These methods will provide the fast and useful information needed for targeting of antimicrobial therapy and appropriate infection control...
- Highly sensitive direct detection and quantification of Burkholderia pseudomallei bacteria in environmental soil samples by using real-time PCR
Trinh Thanh Trung
Friedrich Loeffler Institute of Medical Microbiology, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany
Appl Environ Microbiol 77:6486-94. 2011
..Five samples were negative by both PCR methods and culture. In conclusion, our PCR-based system provides a highly specific and sensitive tool for the quantitative environmental surveillance of B. pseudomallei...
- Acceleration of the direct identification of Staphylococcus aureus versus coagulase-negative staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods
A J M Loonen
PAMM Laboratory for Medical Microbiology, Eindhoven Veldhoven, The Netherlands
Eur J Clin Microbiol Infect Dis 30:337-42. 2011
..An initial S. aureus or CNS load of 1 CFU/ml blood can be detected after 5 h of incubation in BacT/ALERT 3D by combining the sensitive isolation method and the tuf LightCycler assay...
- Rapid, simple, and sensitive detection of Anaplasma phagocytophilum by loop-mediated isothermal amplification of the msp2 gene
Department of Rickettsiology, National Institute of Communicable Disease Control and Prevention, China CDC, Changping, Beijing 102206, People s Republic of China
J Clin Microbiol 49:4117-20. 2011
..phagocytophilum. This LAMP assay is a valuable method for rapid, cost-effective, and simple detection of A. phagocytophilum in the rural areas of China...
- Adjunctive tests for diagnosis of tuberculosis: serology, ELISPOT for site-specific lymphocytes, urinary lipoarabinomannan, string test, and fine needle aspiration
Jacqueline M Achkar
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
J Infect Dis 204:S1130-41. 2011
- Marine methylotrophs revealed by stable-isotope probing, multiple displacement amplification and metagenomics
Josh D Neufeld
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
Environ Microbiol 10:1526-35. 2008
- Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method
Department of Microbiology, IDIBELL Bellvitge Hospital, C Feixa Llarga s n 08907 Hospitalet de Llobregat, Barcelona, Spain
J Clin Microbiol 49:1137-9. 2011
..GX offers high potential for the diagnosis of tuberculosis due to its capacity for direct detection of MTC, its rapidity, and its simplicity...
- Differential mucoid exopolysaccharide production by members of the Burkholderia cepacia complex
James E A Zlosnik
Room 377, Child and Family Research Institute, 950 West 28th Ave, Vancouver, BC V5Z 4H4, Canada
J Clin Microbiol 46:1470-3. 2008
..cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can convert from mucoid to nonmucoid...
- Xpert MTB/RIF for rapid diagnosis of tuberculous lymphadenitis from fine-needle-aspiration biopsy specimens
Louis J Ligthelm
Division of Anatomical Pathology, National Health Laboratory Service, Tygerberg Hospital, Tygerberg, Cape Town, South Africa
J Clin Microbiol 49:3967-70. 2011
..7% (95% confidence interval [CI], 86.6 to 100%) and 88.9% (95% CI, 69.6 to 100%), respectively, and it correctly identified 6/6 (100%) of the cytology smear-negative/culture-positive cases and 1 of 2 (50%) rifampin-resistant cases...
- Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry
Unidad de Investigacion, Hospital Universitario de Salamanca, Salamanca, Spain
J Clin Microbiol 48:2110-5. 2010
..2%). Our results show that MALDI-TOF MS allows bacterial identification directly from infected urine in a short time, with high accuracy, and especially when Gram-negative bacteria with high bacterial counts are involved...
- Economic analysis of the diagnosis of smear-negative pulmonary tuberculosis in South Africa: incorporation of a new rapid test, FASTPlaqueTB, into the diagnostic algorithm
Biotec Laboratories Ltd, c o National Health Laboratory Service, Cape Town, Western Cape, South Africa
Int J Tuberc Lung Dis 8:240-7. 2004
- Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology
Max Planck Institut für Marine Mikrobiologie, Bremen, Germany
FEMS Microbiol Rev 24:555-65. 2000
..In order to speed up the transformation of microbial ecology from a mostly descriptive to a hypothesis-driven, experimental science more intense use must be made of the taxonomic precision and quantitativeness of rRNA-targeted probes...
- Comparison of PCR-ELISA and LightCycler real-time PCR assays for detecting Salmonella spp. in milk and meat samples
Agence Française de Sécurité Sanitaire des Aliments AFSSA, Laboratoire d Etudes et de Recherches sur la Qualité des Aliments et sur les Procédés Agroalimentaires LERQAP, 1 5 rue de Belfort, 94700 Maisons Alfort, France
Mol Cell Probes 18:409-20. 2004
..The results on naturally contaminated samples demonstrated that despite certain inhibition problems, LC-PCR and PCR-ELISA assays were highly specific and sensitive, and provide a powerful tool for detection of Salmonella in food samples...
- Scanty AFB smears: what's in a name?
A Van Deun
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium
Int J Tuberc Lung Dis 8:816-23. 2004
..A tuberculosis control project in Bangladesh...
- Searching for the elusive typhoid diagnostic
Oxford University Clinical Research Unit, The Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam
BMC Infect Dis 10:45. 2010
..Here we discuss the current position of typhoid diagnostics, highlight the need for technological improvements and suggest potential ways of advancing this area...
- Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria
Kathryn E R Davis
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia
Appl Environ Microbiol 71:826-34. 2005
- Rapid classification and identification of salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry
Federal Institute for Risk Assessment, National Salmonella Reference Laboratory, Diedersdorfer Weg 1, D 12277 Berlin, Germany
Appl Environ Microbiol 74:7767-78. 2008
..The resulting mass spectrometry-based bacterial classification was very comparable to the results of DNA sequence-based methods. A rapid protocol that allowed identification of Salmonella subspecies in minutes was established...
- Comparison of recoveries of mycobacterium tuberculosis using the automated BACTEC MGIT 960 system, the BACTEC 460 TB system, and Löwenstein-Jensen medium
Department of Respiratory Medicine, Semmelweis University, H 1536 Budapest 114, Hungary
J Clin Microbiol 38:2395-7. 2000
..In conclusion, the nonradiometric, fully automated 7-ml BACTEC MGIT 960 system can be considered a viable alternative to the semiautomated, radiometric BACTEC 460 TB system...
- PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients
Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, 48109 Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium
J Clin Microbiol 42:2074-9. 2004
..Thus, we have designed two PCR assays: one is specific for the genus Pseudomonas, while the other is specific for P. aeruginosa. Both assays show 100% sensitivity and specificity...
- Detection of microorganisms in blood specimens using matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a review
UMR CNRS, IRD, IFR, Universite de la Mediterranee, Marseille, France
Clin Microbiol Infect 16:1620-5. 2010
- Rapid and simple approach for identification of Mycobacterium tuberculosis complex isolates by PCR-based genomic deletion analysis
Linda M Parsons
Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA
J Clin Microbiol 40:2339-45. 2002
..bovis and M. tuberculosis, when it is important to distinguish between zoonotic sources (i.e., cattle and unpasteurized dairy products) and human sources of tuberculosis disease...
- Diagnosis of Mycobacterium ulcerans infection (Buruli ulcer) at a treatment centre in Ghana: a retrospective analysis of laboratory results of clinically diagnosed cases
Ghana Health Service, Ministry of Health, Tema, Ghana
Trop Med Int Health 13:191-8. 2008
..However, being retrospective, this study did not permit sensitivity and negative predictive value analysis...
- Quantification of bias related to the extraction of DNA directly from soils
Laboratoire d Ecologie Microbienne du Sol, UMR CNRS 5557, Universite Claude Bernard Lyon 1, F 69622 Villeurbanne Cedex, France
Appl Environ Microbiol 65:5409-20. 1999
..We also investigated different purification techniques and optimized the PCR methods in order to develop a protocol based on hybridization of the PCR products and quantification by phosphorimaging...
- Meta-analysis of BACTEC MGIT 960 and BACTEC 460 TB, with or without solid media, for detection of mycobacteria
HIV Outpatient Clinic, Center of Preventive Medicine, Verona, Italy
J Clin Microbiol 42:2321-5. 2004
..5 and 99.6% and 85.8 and 99.9%, respectively. Combined with solid media, the sensitivity of the two systems increased to 87.7 and 89.7%, respectively...
- Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting
Department of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg Eppendorf, Martinistr 52, 20246 Hamburg, Germany
J Clin Microbiol 48:1584-91. 2010
..With turnaround times around 100 min, the approach allowed for reliable pathogen identification at the day of blood culture positivity, providing treatment-relevant information within the critical phase of septic illness...
- Point-of-care Xpert® MTB/RIF for smear-negative tuberculosis suspects at a primary care clinic in South Africa
A van Rie
Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Int J Tuberc Lung Dis 17:368-72. 2013
..To assess the clinical utility and cost of point-of-care Xpert® MTB/RIF for the diagnosis of smear-negative tuberculosis (TB)...
- Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Unidad de Investigacion, Hospital Universitario de Salamanca, Salamanca, Spain
Clin Microbiol Infect 17:546-51. 2011
- Sputum monitoring during tuberculosis treatment for predicting outcome: systematic review and meta-analysis
David J Horne
Division of Pulmonary and Critical Care Medicine, Seattle, WA 98104, USA
Lancet Infect Dis 10:387-94. 2010
..Although we pooled a diverse group of patients, the individual studies had similar performance characteristics. Better predictive markers are needed...
- Optimisation of storage conditions for maintaining culturability of penicillin-susceptible and penicillin-resistant isolates of Streptococcus pneumoniae in transport medium
C K Mason
Oral Research Centre, School of Dentistry, Queen s University, Belfast, Northern Ireland, UK
Br J Biomed Sci 67:1-4. 2010
..This work will help to establish robust sampling protocols when performing community studies to ensure culturability of comparison between community and laboratory pneumococci survival...
- Haemophilus influenzae outer membrane protein P6 molecular characterization may not differentiate all strains of H. Influenzae from H. haemolyticus
Rochester General Hospital, Rochester General Research Institute, Center for Infectious Diseases and Immunology, Rochester, NY 14621, USA
J Clin Microbiol 48:3756-7. 2010
..Further testing using RapID NH and multilocus sequence analysis may not help identify some isolates. Translated OMP P6 gene sequences are not conserved among all isolates presumed to be H. influenzae...
- Novel chemically modified liquid medium that will support the growth of seven bartonella species
Ricardo G Maggi
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
J Clin Microbiol 43:2651-5. 2005
..This liquid growth medium may provide an advantage over conventional direct blood agar plating for the diagnostic confirmation of bartonellosis...
- Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR
Laboratoire de bactériologie virologie hygiène, Hopital Henri Mondor, Assistance Publique Hopitaux de Paris, Creteil, France
Ann Clin Microbiol Antimicrob 9:21. 2010
..The present study was aimed to develop a novel quantitative PCR (qPCR) assay, using ecfX as the specific target gene, for the rapid and accurate identification of P. aeruginosa from positive blood cultures (BCs)...
- Laboratory diagnosis of Buruli ulcer disease
Department of Infectious Diseases and Tropical Medicine, Ludwig Maximilians University Munich, Leopoldstrasse 5, 80802 Munich, Germany
Future Microbiol 5:363-70. 2010
..Currently, cultures are considered the only valid confirmatory test for the detection of viable bacilli...
- Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens
Department of Microbiology, Our Lady s Children s Hospital, Crumlin, Dublin, Ireland
Diagn Microbiol Infect Dis 68:358-65. 2010
..aeruginosa culture results...
- Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets
Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland
J Clin Microbiol 48:1481-3. 2010
..Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising...
- Evaluation of tuberculosis diagnostics: establishing an evidence base around the public health impact
Richard J Lessells
Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, UK
J Infect Dis 204:S1187-95. 2011
..The urgent need for new diagnostics should not distract from pursuing rigorous scientific evaluation focused on public health impact...
- Rapid discrimination of environmental Vibrio by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
UPRES EA 4438, Physiopathologie et Médecine Translationnelle, Laboratoire de Bactériologie de la Faculté de Médecine, Universite de Strasbourg, Hopitaux Universitaires de Strasbourg, 3 Rue Koeberle, 67000 Strasbourg, France
Microbiol Res 167:226-30. 2012
..The results of MALDI TOF analysis show a high congruence of strain grouping demonstrating the accuracy and reliability of MALDI-TOF MS...
- Yield of acid-fast smear and mycobacterial culture for tuberculosis diagnosis in people with human immunodeficiency virus
Thailand Ministry of Public Health U S CDC Collaboration, Nonthaburi, Thailand
Am J Respir Crit Care Med 180:903-8. 2009
..The World Health Organization recently revised its recommendations for tuberculosis (TB) diagnosis in people with HIV. Most studies cited to support these policies involved HIV-uninfected patients and only evaluated sputum specimens...
- Selective detection of live bacteria combining propidium monoazide sample treatment with microarray technology
Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA
J Microbiol Methods 76:253-61. 2009
..Minimization of detection signals deriving from dead cells will render data more relevant in studies including pathogen risk assessment...
- Evaluation of a PCR test for detection of treponema pallidum in swabs and blood
P A Grange
Laboratoire de Recherche en Dermatologie, EA 1833, Centre National de Référence Syphilis, Faculte de Medecine, Universite Paris Descartes, Paris, France
J Clin Microbiol 50:546-52. 2012
..pallidum detection in any of the specimens tested. Swab specimens from mucosal or skin lesions seemed to be more useful than blood for the efficient detection of the T. pallidum genome and, thus, for the diagnosis of syphilis...
- Detection of Mycobacterium tuberculosis in blood by use of the Xpert MTB/RIF assay
Padmapriya P Banada
Center for Infectious Diseases, New Jersey Medical School UMDNJ, Newark, New Jersey, USA
J Clin Microbiol 51:2317-22. 2013
..tuberculosis. This approach may be a useful method to detect extrapulmonary tuberculosis and the risk of death in immunocompromised patients...
- Development of a new, combined rapid method using phage and PCR for detection and identification of viable Mycobacterium paratuberculosis bacteria within 48 hours
Emma C Stanley
Division of Food Sciences, School of Biosciences, Sutton Bonington Campus, University of Nottingham, Sutton Bonington LE12 5RD, United Kingdom
Appl Environ Microbiol 73:1851-7. 2007
..Combining the plaque PCR technique with the phage-based detection assay allowed the rapid and specific detection of viable M. avium subsp. paratuberculosis in milk samples in just 48 h...
- Neurotoxin gene profiling of clostridium botulinum types C and D native to different countries within Europe
ANSES French Agency for Food, Environmental and Occupational Health Safety, Food Safety Laboratory, Maisons Alfort, France
Appl Environ Microbiol 78:3120-7. 2012
..Further investigations should now be performed in order to check that mosaic types dominate in Europe and that acquisition of mosaic types helps in survival or adaptation to particular niche...
- Comparison of BD GeneOhm real-time polymerase chain reaction with chromogenic and conventional culture methods for detection of group B Streptococcus in clinical samples
Microbiology Department, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK
Diagn Microbiol Infect Dis 61:369-72. 2008
..5% versus 57% respectively, P < 0.02). After selective enrichment, 92.8% of GBS were isolated on chromID Strepto B compared with 89.2% isolated on sheep blood agar...
- Microscopic-observation drug-susceptibility assay for the diagnosis of TB
David A J Moore
Department of Infectious Diseases and Immunity and the Wellcome Centre for Clinical Tropical Medicine, Imperial College London, Faculty of Medicine Hammersmith Campus, London, United Kingdom
N Engl J Med 355:1539-50. 2006
- An integrated approach to rapid diagnosis of tuberculosis and multidrug resistance using liquid culture and molecular methods in Russia
National Mycobacterium Reference Laboratory, Institute of Cell and Molecular Sciences, Queen Mary College, Barts and the London School of Medicine, University of London, London, United Kingdom
PLoS ONE 4:e7129. 2009
..To analyse the feasibility, cost and performance of rapid tuberculosis (TB) molecular and culture systems, in a high multidrug-resistant TB (MDR TB) middle-income region (Samara, Russia) and provide evidence for WHO policy change...
- Evaluation of a rapid, real-time intrapartum group B streptococcus assay
Brett C Young
Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
Am J Obstet Gynecol 205:372.e1-6. 2011
..We sought to evaluate an intrapartum nucleic acid amplification test (NAAT) for group B streptococcus (GBS)...
- Rapid identification of Legionella spp. by MALDI-TOF MS based protein mass fingerprinting
Cantonal Institute of Microbiology, Bellinzona, Switzerland
Syst Appl Microbiol 34:40-4. 2011
..The identification of Legionella spp. by MALDI-TOF MS is rapid, easy to perform and has the advantage of being time- and cost-saving, in comparison to sequence-based identification...
- Diagnosis of acute leptospirosis
Field Epidemiology Training Program Japan, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, Japan
Expert Rev Anti Infect Ther 9:111-21. 2011
..will accelerate our understanding of Leptospira pathogenesis and provide insights into the development of more efficient and accurate diagnostic tests for acute-phase leptospirosis...
- Comparison of premier CAMPY enzyme immunoassay (EIA), ProSpecT Campylobacter EIA, and ImmunoCard STAT! CAMPY tests with culture for laboratory diagnosis of Campylobacter enteric infections
Paul A Granato
Department of Microbiology and Immunology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
J Clin Microbiol 48:4022-7. 2010
..jejuni and C. coli and that conventional culture may no longer be recognized as the "gold standard" for diagnosis...
- Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs
Lance R Peterson
NorthShore University Healthsystem, Department of Pathology and Laboratory Medicine, 2650 Ridge Avenue, Walgreen Bldg, SB525, Evanston, IL 60201, USA
J Clin Microbiol 48:1661-6. 2010
..001) with the LightCycler MRSA advanced test. The sensitivity of direct culture was 80.4%. The LightCycler MRSA advanced test is a useful tool for sensitive and rapid detection of MRSA nasal colonization...
- Contribution of amoebic coculture to recovery of Legionella isolates from respiratory samples: prospective analysis over a period of 32 months
Universite de Lyon, Lyon, France
J Clin Microbiol 50:1725-6. 2012
..1%. Amoebic coculture was particularly efficient in isolating Legionella spp. from respiratory samples contaminated with oropharyngeal flora...
- Maximizing capture efficiency and specificity of magnetic separation for Mycobacterium avium subsp. paratuberculosis cells
Institute of Agri food and Land Use, School of Biological Sciences, Queen s University Belfast, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland
Appl Environ Microbiol 76:7550-8. 2010
..3 PFU/ml). This PMS-phage assay represents a novel, rapid method for the detection and enumeration of viable M. avium subsp. paratuberculosis organisms in milk, and potentially other sample matrices, with results available within 48 h...
- Comparison of MALDI TOF with conventional identification of clinically relevant bacteria
Labormedizinisches zentrum Dr Risch, Liebefeld, Switzerland
Swiss Med Wkly 140:w13095. 2010
..Comparison between results of the new procedure with those obtained by conventional testing is mandatory...
- Identification of HACEK clinical isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry
Marc Roger Couturier
University of Utah School of Medicine, Department of Pathology, Salt Lake City, UT 84108, USA
J Clin Microbiol 49:1104-6. 2011
..Ninety-three percent of HACEK organisms were identified correctly to the genus level using the Bruker database, and 100% were identified to the genus level using a custom database that included clinical isolates...
- Direct amplification of rRNA genes in diagnosis of bacterial infections
Department of Medical Microbiology, University of Turku, Turku, Finland
J Clin Microbiol 38:32-9. 2000
- Clinical value of Treponema pallidum real-time PCR for diagnosis of syphilis
Public Health Laboratory, Amsterdam Public Health Service, Amsterdam, The Netherlands
J Clin Microbiol 48:497-502. 2010
- A new multiplex PCR for differential identification of Shigella flexneri and Shigella sonnei and detection of Shigella virulence determinants
M J Farfán
Departamento de Pediatria, Hospital Dr Luis Calvo Mackenna, Facultad de Medicina, Universidad de Chile, Santiago, Chile
Epidemiol Infect 138:525-33. 2010
..flexneri and S. sonnei. The technique was sensitive, reproducible, specific and simple to perform, providing a new tool with the potential to be employed for epidemiological and diagnostic purposes...
- Laboratory practices and incidence of non-O157 shiga toxin-producing Escherichia coli infections
Kathleen A Stigi
Washington State Department of Health, Shoreline, WA 98155, USA
Emerg Infect Dis 18:477-9. 2012
..Despite increased assay use, only half of processed stool specimens underwent Shiga toxin testing during 2010, suggesting substantial underdetection of non-O157 STEC infections...
- Performance of a rapid antigen-detection test and throat culture in community pediatric offices: implications for management of pharyngitis
Robert R Tanz
Department of Pediatrics, Children s Memorial Hospital and Northwestern University Feinberg School of Medicine, Chicago, IL 60614, USA
Pediatrics 123:437-44. 2009
- Rapid genus- and species-specific identification of Cronobacter spp. by matrix-assisted laser desorption ionization-time of flight mass spectrometry
Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstr 272, CH 8057 Zurich, Switzerland
J Clin Microbiol 48:2846-51. 2010
..Our study demonstrates that MALDI-TOF MS is a reliable and powerful tool for the rapid identification of Cronobacter strains to the genus and species level...
- Costs and cost-effectiveness of tuberculosis cultures using solid and liquid media in a developing country
D H Mueller
Health Economics and Financing Programme, Department of Public Health and Policy, London School of Hygiene and Tropical Medicine, London, UK
Int J Tuberc Lung Dis 12:1196-202. 2008
..The expansion of culture has been proposed to aid tuberculosis (TB) control in developing countries...
- Three-hour molecular detection of Campylobacter, Salmonella, Yersinia, and Shigella species in feces with accuracy as high as that of culture
Scott A Cunningham
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First St S W, Rochester, MN 55905, USA
J Clin Microbiol 48:2929-33. 2010
..Paired with reflexive culture of stools testing positive, this should provide an improvement in care for patients with acute infectious diarrheal disease...
- Does bleach processing increase the accuracy of sputum smear microscopy for diagnosing pulmonary tuberculosis?
Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital, University of California, San Francisco, CA 94110, USA
J Clin Microbiol 48:2433-9. 2010
..Further research should focus on alternative approaches to optimizing smear microscopy, such as light-emitting diode fluorescence microscopy and same-day sputum collection strategies...
- Diagnosis of pulmonary tuberculosis in children: new advances
Heather J Zar
Department of Paediatrics and Child Health, Red Cross War Memorial Children s Hospital, University of Cape Town, South Africa
Expert Rev Anti Infect Ther 8:277-88. 2010
..Greater efforts to obtain a microbiologic diagnosis should be made in children, even in primary care settings. Further research to develop a more accurate, cost-effective and simple diagnostic test for childhood TB is urgently needed...
- PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis
Department of Medicine E, Rabin Medical Center, Belinson Hospital, Ramat Aviv, Israel
J Clin Microbiol 48:489-96. 2010
..Currently available methods for PCR with blood samples for the diagnosis of IPD lack the sensitivity and specificity necessary for clinical practice...
- Accuracy of identification and susceptibility results by direct inoculation of Vitek 2 cards from positive BACTEC cultures
J J Kerremans
Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre Rotterdam, The Netherlands
Eur J Clin Microbiol Infect Dis 23:892-8. 2004
..Susceptibility results for this antibiotic, if obtained using direct inoculation, should not be reported to the clinician...
- Molecular analysis improves pathogen identification and epidemiologic study of pediatric parapneumonic empyema
Anne J Blaschke
Department of Pediatrics, University of Utah, Salt, Lake City, UT, USA
Pediatr Infect Dis J 30:289-94. 2011
..Epidemiologic study is complicated by the low frequency of positive cultures. We sought to describe the epidemiology of PPE in children using molecular analysis of pleural fluid...
- Usefulness of real-time PCR for lytA, ply, and Spn9802 on plasma samples for the diagnosis of pneumococcal pneumonia
Department of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden
Clin Microbiol Infect 16:1135-41. 2010
- Investigation of biofilm formation in clinical isolates of Staphylococcus aureus
James E Cassat
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR, USA
Methods Mol Biol 391:127-44. 2007
..However, because this system is not generally available, we restrict technical comments in this chapter to our use of an implanted catheter model evaluated by direct microbio-logical analysis of explanted catheters (2)...
- Burkholderia pseudomallei misidentified by automated system
Institute for Clinical Microbiology and Immunology, St Gallen CH 9001, Switzerland
Emerg Infect Dis 15:1799-801. 2009
..The organism was eventually identified by molecular methods as B. pseudomallei...
- Broad diversity of viable bacteria in 'sterile' (0.2 microm) filtered water
Martin W Hahn
Institute for Limnology, Austrian Academy of Sciences, Mondseestrasse 9, 5310 Mondsee, Austria
Res Microbiol 155:688-91. 2004
..2-microm filters, and it is concluded that microbiologists may have dramatically underestimated the diversity of 0.2 microm-filterable bacteria...
- Evaluation of BACTEC Mycobacteria Growth Indicator Tube (MGIT 960) automated system for drug susceptibility testing of Mycobacterium tuberculosis
Istituto di Microbiologia, , Rome, Italy
J Clin Microbiol 39:4440-4. 2001
..05). In conclusion, the performance of BACTEC MGIT 960 was found similar to that of BACTEC 460 TB and this new system can be considered a good alternative to the radiometric method for routine susceptibility testing of M. tuberculosis...
- Interactions and self-organization in the soil-microbe complex
I M Young
Scottish Informatics, Mathematics, Biology, and Statistics SIMBIOS Centre, University of Abertay, Bell Street, Dundee, DD1 1HG Scotland, UK
Science 304:1634-7. 2004
..We introduce the concept that the soil-microbe system is self-organized and suggest new priorities for research based on an integrative approach that combines biochemistry and biophysics...
- Comparison of rapid intrapartum screening methods for group B streptococcal vaginal colonization
Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA 94025, USA
J Matern Fetal Neonatal Med 18:225-9. 2005
..To compare optical immunoassay (OIA) and rapid polymerase-chain reaction (PCR) with enrichment broth culture for intrapartum detection of vaginal group B streptococcal (GBS) colonization...
- Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays
D M Wolk
University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
J Clin Microbiol 47:823-6. 2009
..aureus. Sensitivity was 97.1% and 98.3% for MRSA in wound and blood culture specimens, respectively. Sensitivity was 100% for S. aureus from both specimen types...
- Improved reliability of Pseudomonas aeruginosa PCR detection by the use of the species-specific ecfX gene target
Research group on Bacterial Opportunistic Pathogens and Environment Université de Lyon, Lyon, F 69003, France
J Microbiol Methods 70:20-9. 2007
..aeruginosa. These investigations suggest a preferential colonization of water rather than soil environments by P. aeruginosa. Detection limits of P. aeruginosa in environmental samples were improved by the ecfX PCR screening...
- Optimization of media for detection of hydrogen peroxide production by Lactobacillus species
L K Rabe
Magee Women s Research Institute, The University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
J Clin Microbiol 41:3260-4. 2003
..This new medium better supports the growth of a wider range of Lactobacillus strains isolated from the vagina and enhances the color production of H(2)O(2)-producing strains...
- Cultivation-dependent and -independent approaches for determining bacterial diversity in heavy-metal-contaminated soil
Richard J Ellis
NERC Centre for Population Biology, Imperial College London, Ascot, Berkshire SL5 7PY, United Kingdom
Appl Environ Microbiol 69:3223-30. 2003
..Thus, it appears that plate counts may be a more appropriate method for determining the effect of heavy metals on soil bacteria than culture-independent approaches...