patch clamp techniques

Summary

Summary: An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.

Top Publications

  1. ncbi Synaptic amplifier of inflammatory pain in the spinal dorsal horn
    Hiroshi Ikeda
    Department of Neurophysiology, Center for Brain Research, Medical University of Vienna, Vienna, Austria
    Science 312:1659-62. 2006
  2. ncbi Somatosensory integration controlled by dynamic thalamocortical feed-forward inhibition
    Laetitia Gabernet
    Brain Research Institute, University of Zurich, 8057 Zurich, Switzerland
    Neuron 48:315-27. 2005
  3. pmc A severe defect in CRAC Ca2+ channel activation and altered K+ channel gating in T cells from immunodeficient patients
    Stefan Feske
    CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 202:651-62. 2005
  4. ncbi Synaptic basis for intense thalamocortical activation of feedforward inhibitory cells in neocortex
    Scott J Cruikshank
    Department of Neuroscience, Division of Biology and Medicine, Box G LN, Brown University, Providence, Rhode Island 02912, USA
    Nat Neurosci 10:462-8. 2007
  5. ncbi Insect olfactory receptors are heteromeric ligand-gated ion channels
    Koji Sato
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    Nature 452:1002-6. 2008
  6. ncbi Mechanism of calcium gating in small-conductance calcium-activated potassium channels
    X M Xia
    Vollum Institute, Oregon Health Sciences University, Portland 97201, USA
    Nature 395:503-7. 1998
  7. ncbi Enforcement of temporal fidelity in pyramidal cells by somatic feed-forward inhibition
    F Pouille
    Brain Research Institute, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
    Science 293:1159-63. 2001
  8. ncbi A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel
    M C Sanguinetti
    Eccles Program in Human Molecular Biology and Genetics, University of Utah Health Sciences Center, Salt Lake City 84112, USA
    Cell 81:299-307. 1995
  9. ncbi A TRP channel that senses cold stimuli and menthol
    Andrea M Peier
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Cell 108:705-15. 2002
  10. ncbi Synfire chains and cortical songs: temporal modules of cortical activity
    Yuji Ikegaya
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Science 304:559-64. 2004

Research Grants

Detail Information

Publications324 found, 100 shown here

  1. ncbi Synaptic amplifier of inflammatory pain in the spinal dorsal horn
    Hiroshi Ikeda
    Department of Neurophysiology, Center for Brain Research, Medical University of Vienna, Vienna, Austria
    Science 312:1659-62. 2006
    ..This model integrates known signal transduction pathways of hyperalgesia without contradiction...
  2. ncbi Somatosensory integration controlled by dynamic thalamocortical feed-forward inhibition
    Laetitia Gabernet
    Brain Research Institute, University of Zurich, 8057 Zurich, Switzerland
    Neuron 48:315-27. 2005
    ..Hence, feed-forward inhibitory circuits, classically known to sharpen spatial contrast of tactile inputs, also increase the temporal resolution in the somatosensory cortex...
  3. pmc A severe defect in CRAC Ca2+ channel activation and altered K+ channel gating in T cells from immunodeficient patients
    Stefan Feske
    CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 202:651-62. 2005
    ..They also offer evidence for a functional link between CRAC and Kv1.3 channels, and establish a model system for molecular genetic studies of the CRAC channel...
  4. ncbi Synaptic basis for intense thalamocortical activation of feedforward inhibitory cells in neocortex
    Scott J Cruikshank
    Department of Neuroscience, Division of Biology and Medicine, Box G LN, Brown University, Providence, Rhode Island 02912, USA
    Nat Neurosci 10:462-8. 2007
    ..These results demonstrate the importance of selective synaptic targeting and precise timing in the initial stages of neocortical processing...
  5. ncbi Insect olfactory receptors are heteromeric ligand-gated ion channels
    Koji Sato
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    Nature 452:1002-6. 2008
    ..The ligand-gated ion channel formed by an insect OR complex seems to be the basis for a unique strategy that insects have acquired to respond to the olfactory environment...
  6. ncbi Mechanism of calcium gating in small-conductance calcium-activated potassium channels
    X M Xia
    Vollum Institute, Oregon Health Sciences University, Portland 97201, USA
    Nature 395:503-7. 1998
    ..Our data support a model in which calcium gating of SK channels is mediated by binding of calcium to calmodulin and subsequent conformational alterations in the channel protein...
  7. ncbi Enforcement of temporal fidelity in pyramidal cells by somatic feed-forward inhibition
    F Pouille
    Brain Research Institute, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
    Science 293:1159-63. 2001
    ..Thus, the subcellular partitioning of feed-forward inhibition enforces precise coincidence detection in the soma, while allowing dendrites to sum incoming activity over broader time windows...
  8. ncbi A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel
    M C Sanguinetti
    Eccles Program in Human Molecular Biology and Genetics, University of Utah Health Sciences Center, Salt Lake City 84112, USA
    Cell 81:299-307. 1995
    ..Since block of IKr is a known mechanism for drug-induced cardiac arrhythmias, the finding that HERG encodes IKr channels provides a mechanistic link between certain forms of inherited and acquired LQT...
  9. ncbi A TRP channel that senses cold stimuli and menthol
    Andrea M Peier
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Cell 108:705-15. 2002
    ..Our identification of a cold-sensing TRP channel in a distinct subpopulation of sensory neurons implicates an expanded role for this family of ion channels in somatic sensory detection...
  10. ncbi Synfire chains and cortical songs: temporal modules of cortical activity
    Yuji Ikegaya
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Science 304:559-64. 2004
    ..Sequences of active neurons have distinct spatial structures and are repeated in the same order over tens of seconds, revealing modular temporal dynamics. Higher order sequences are replayed with compressed timing...
  11. pmc Physiology and anatomy of synaptic connections between thick tufted pyramidal neurones in the developing rat neocortex
    H Markram
    Max Planck Institut fur medizinische Forschung, Abteilung Zellphysiologie, Germany
    J Physiol 500:409-40. 1997
    ..neurones, which have their synaptic contacts preferentially located on basal and apical oblique dendrites, was due to differences in transmitter release probability of the projecting neurones and, to a lesser extent, to differenc..
  12. ncbi KCNQ2 and KCNQ3 potassium channel subunits: molecular correlates of the M-channel
    H S Wang
    Institute of Molecular Cardiology, Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, NY 11794, USA
    Science 282:1890-3. 1998
    ..The biophysical properties, sensitivity to pharmacological blockade, and expression pattern of the KCNQ2 and KCNQ3 potassium channels were determined. It is concluded that both these subunits contribute to the native M-current...
  13. ncbi Structural basis for modulation and agonist specificity of HCN pacemaker channels
    William N Zagotta
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, Box 357290, University of Washington School of Medicine, Seattle, Washington 98195 7290, USA
    Nature 425:200-5. 2003
    ..On the basis of amino acid sequence similarity to HCN channels, the cyclic nucleotide-gated, and eag- and KAT1-related families of channels are probably related to HCN channels in structure and mechanism...
  14. pmc Cytokine mechanisms of central sensitization: distinct and overlapping role of interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha in regulating synaptic and neuronal activity in the superficial spinal cord
    Yasuhiko Kawasaki
    Pain Research Center, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Neurosci 28:5189-94. 2008
    ..PICs may further induce long-term synaptic plasticity through CREB-mediated gene transcription. Blockade of PIC signaling could be an effective way to suppress central sensitization and alleviate chronic pain...
  15. ncbi HERG, a human inward rectifier in the voltage-gated potassium channel family
    M C Trudeau
    Department of Physiology, University of Wisconsin Medical School, Madison 53706, USA
    Science 269:92-5. 1995
    ..Because mutations in HERG cause a form of long-QT syndrome, these properties of HERG channel function may be critical to the maintenance of normal cardiac rhythmicity...
  16. ncbi Astrocytes potentiate transmitter release at single hippocampal synapses
    Gertrudis Perea
    Instituto Cajal, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    Science 317:1083-6. 2007
    ..This persistent plasticity was mGluR-mediated but N-methyl-d-aspartate receptor-independent. These results indicate that astrocytes are actively involved in the transfer and storage of synaptic information...
  17. ncbi Small-conductance, calcium-activated potassium channels from mammalian brain
    M Kohler
    Vollum Institute, L 474, Oregon Health Sciences University, 3181 Southwest Sam Jackson Road, Portland, OR 97201, USA J Maylie, Department of Obstetrics and Gyne
    Science 273:1709-14. 1996
    ....
  18. ncbi Distinct functional and pharmacological properties of tonic and quantal inhibitory postsynaptic currents mediated by gamma-aminobutyric acid(A) receptors in hippocampal neurons
    D Bai
    Department of Physiology, University of Toronto, Toronto, Ontario, Canada
    Mol Pharmacol 59:814-24. 2001
    ..In summary, the receptors underlying the tonic current are functionally and pharmacologically distinct from quantally activated synaptic receptors and these receptors represent a novel target for neurodepressive drugs...
  19. ncbi Glutamate exocytosis from astrocytes controls synaptic strength
    Pascal Jourdain
    Department of Cell Biology and Morphology, University of Lausanne, Rue du Bugnon 9, 1005 Lausanne, Switzerland
    Nat Neurosci 10:331-9. 2007
    ..Thus, we provide the first combined functional and ultrastructural evidence for a physiological control of synaptic activity via exocytosis of glutamate from astrocytes...
  20. pmc Tracking voltage-dependent conformational changes in skeletal muscle sodium channel during activation
    Baron Chanda
    Department of Physiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    J Gen Physiol 120:629-45. 2002
    ..These experiments allow us to map structural features onto the kinetic landscape of a sodium channel during activation...
  21. ncbi Cloning and functional characterization of novel large conductance calcium-activated potassium channel beta subunits, hKCNMB3 and hKCNMB4
    R Brenner
    Howard Hughes Medical Institute, Molecular and Cellular Physiology, Stanford School of Medicine, Stanford, California 94305, USA
    J Biol Chem 275:6453-61. 2000
    ..These results suggest that beta subunits in diverse tissue types fine-tune slo channel properties to the needs of a particular cell...
  22. ncbi Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol
    T Hofmann
    Institut fur Pharmakologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Germany
    Nature 397:259-63. 1999
    ..Thus, hTRPC3 and hTRPC6 represent the first members of a new functional family of second-messenger-operated cation channels, which are activated by diacylglycerol...
  23. pmc Properties of hyperpolarization-activated pacemaker current defined by coassembly of HCN1 and HCN2 subunits and basal modulation by cyclic nucleotide
    S Chen
    Department of Pharmacology, ColumbiaUniversity, New York, New York 10032, USA
    J Gen Physiol 117:491-504. 2001
    ..Finally, differences in Ih channel properties recorded in cell-free patches versus intact oocytes are shown to be due, in part, to modulation of Ih by basal levels of cAMP in intact cells...
  24. ncbi Activity deprivation reduces miniature IPSC amplitude by decreasing the number of postsynaptic GABA(A) receptors clustered at neocortical synapses
    Valerie Kilman
    Department of Biology and Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02454, USA
    J Neurosci 22:1328-37. 2002
    ..Because excitatory and inhibitory synaptic currents are regulated in opposite directions by activity blockade, these data suggest that the balance between excitation and inhibition is dynamically regulated by ongoing activity...
  25. ncbi Molecular and functional heterogeneity of hyperpolarization-activated pacemaker channels in the mouse CNS
    B Santoro
    Center for Neurobiology and Behavior, Departments of Pharmacology and Anesthesiology, and Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA
    J Neurosci 20:5264-75. 2000
    ..Moreover, in some neurons, specific functional roles can be proposed for I(h) channels with defined subunit composition...
  26. ncbi Acid-sensing ion channel 1 is localized in brain regions with high synaptic density and contributes to fear conditioning
    John A Wemmie
    Department of Psychiatry, Neuroscience Graduate Program, Howard Hughes Medical Institute, University of Iowa, Iowa City, Iowa 52242, USA
    J Neurosci 23:5496-502. 2003
    ..These studies suggest that ASIC1 is distributed to regions supporting high levels of synaptic plasticity and contributes to the neural mechanisms of fear conditioning...
  27. pmc Correlations between neuronal morphology and electrophysiological features in the rodent superficial dorsal horn
    T J Grudt
    Department of Cell and Molecular Physiology, University of North Carolina CH, Chapel Hill, NC 27599 7545, USA
    J Physiol 540:189-207. 2002
    ....
  28. ncbi Role of gap junctions in synchronized neuronal oscillations in the inferior olive
    Elena Leznik
    Department of Physiology and Neuroscience, New York University Medical School, NY 10016, USA
    J Neurophysiol 94:2447-56. 2005
    ..The findings support the view that oscillatory properties of single IO neurons endow the system with important reset dynamics, while gap junctions are mainly required for synchronized neuronal ensemble activity...
  29. ncbi Released fraction and total size of a pool of immediately available transmitter quanta at a calyx synapse
    R Schneggenburger
    Max Planck Institut fur biophysikalische Chemie, Abteilung Membranbiophysik, Gottingen, Germany
    Neuron 23:399-409. 1999
    ..The results reveal a surprisingly large pool of functionally available vesicles, of which a fraction of about 0.2 is released by a single presynaptic action potential under physiological conditions...
  30. ncbi Remote control of neuronal activity with a light-gated glutamate receptor
    Stephanie Szobota
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Neuron 54:535-45. 2007
    ..Our studies show that LiGluR provides robust control over neuronal activity, enabling the dissection and manipulation of neural circuitry in vivo...
  31. pmc The S4-S5 linker couples voltage sensing and activation of pacemaker channels
    J Chen
    Department of Medicine, Division of Cardiology, Eccles Program in Human Molecular Biology and Genetics, University of Utah, Eccles Institute of Human Genetics, 15 N 2030 E, Room 4220, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 98:11277-82. 2001
    ..Our findings also suggest that opening of HCN and related channels corresponds to activation of a gate located near the inner pore, rather than recovery of channels from a C-type inactivated state...
  32. pmc STIM1, an essential and conserved component of store-operated Ca2+ channel function
    Jack Roos
    Torrey Pines Therapeutics, Inc, La Jolla, CA 92037, USA
    J Cell Biol 169:435-45. 2005
    ..We propose that STIM1, a ubiquitously expressed protein that is conserved from Drosophila to mammalian cells, plays an essential role in SOC influx and may be a common component of SOC and CRAC channels...
  33. pmc Pharmacological characterization of a novel cell line expressing human alpha(4)beta(3)delta GABA(A) receptors
    N Brown
    Merck Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Terlings Park, Eastwick Road, Harlow, Essex CM20 2QR, UK
    Br J Pharmacol 136:965-74. 2002
    ..With its restricted distribution within the brain and unique pharmacology this receptor may play an important role in the action of neurosteroids and anaesthetics. British Journal of Pharmacology (2002) 136, 965-974..
  34. ncbi A novel approach to in situ characterization of pancreatic beta-cells
    Stephan Speier
    European Neuroscience Institute Göttingen, Waldweg 33, 37073 Gottingen, Germany
    Pflugers Arch 446:553-8. 2003
    ..The pancreatic tissue slice preparation is a novel method for studying the function of the beta- and other pancreatic endocrine and exocrine cells under near-physiological conditions...
  35. ncbi Truncation of Kir6.2 produces ATP-sensitive K+ channels in the absence of the sulphonylurea receptor
    S J Tucker
    University Laboratory of Physiology, Oxford, UK
    Nature 387:179-83. 1997
    ..We show here that the primary site at which ATP acts to mediate K-ATP channel inhibition is located on Kir6.2, and that SUR1 is required for sensitivity to sulphonylureas and diazoxide and for activation by Mg-ADP...
  36. ncbi TRP-PLIK, a bifunctional protein with kinase and ion channel activities
    L W Runnels
    Howard Hughes Medical Institute, Department of Cardiology, Department of Neurobiology, Harvard Medical School, 1309 Enders Building, 320 Longwood Avenue, Children s Hospital, Boston, MA 02115, USA
    Science 291:1043-7. 2001
    ..Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function...
  37. ncbi Peripheral inflammation sensitizes P2X receptor-mediated responses in rat dorsal root ganglion neurons
    Guang Yin Xu
    Marine Biomedical Institute and Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas 77555 1069, USA
    J Neurosci 22:93-102. 2002
    ..These results suggest that P2X receptors are useful targets for inflammatory pain therapy...
  38. ncbi Spike timing of distinct types of GABAergic interneuron during hippocampal gamma oscillations in vitro
    Norbert Hajos
    Department of Cellular and Network Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest H 1450, Hungary
    J Neurosci 24:9127-37. 2004
    ....
  39. ncbi Neural substrates of awakening probed with optogenetic control of hypocretin neurons
    Antoine R Adamantidis
    Department of Psychiatry and Behavioral Sciences, Stanford University, 701B Welch Road, Palo Alto, California 94304, USA
    Nature 450:420-4. 2007
    ..This study establishes a causal relationship between frequency-dependent activity of a genetically defined neural cell type and a specific mammalian behaviour central to clinical conditions and neurobehavioural physiology...
  40. ncbi Multiple forms of synaptic plasticity triggered by selective suppression of activity in individual neurons
    Juan Burrone
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 420:414-8. 2002
    ..Our results highlight the differences between global and selective suppression of activity, as well as those between early and late manipulation of activity...
  41. pmc Restoration of single-channel currents using the segmental k-means method based on hidden Markov modeling
    Feng Qin
    Department of Physiology and Biophysical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Biophys J 86:1488-501. 2004
    ..The approach is applicable to data containing subconductance levels or multiple channels and permits state-dependent noises. Examples are given to elucidate its performance and practical applicability...
  42. ncbi Calcium signaling mechanisms in T lymphocytes
    R S Lewis
    Department of Molecular and Cellular Physiology and Program in Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Annu Rev Immunol 19:497-521. 2001
    ..The complexity of Ca(2+) signals contains a wealth of information that may help to instruct lymphocytes to choose between alternate fates in response to antigenic stimulation...
  43. ncbi Altered electrical properties in Drosophila neurons developing without synaptic transmission
    R A Baines
    Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ United Kingdom
    J Neurosci 21:1523-31. 2001
    ....
  44. pmc Dopamine D1/D5 receptor modulation of excitatory synaptic inputs to layer V prefrontal cortex neurons
    J K Seamans
    Howard Hughes Medical Institute and Molecular and Computational Neurobiology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 98:301-6. 2001
    ..By increasing the NMDA component of EPSCs, yet slightly reducing release, D1/D5 receptor activation selectively enhanced sustained synaptic inputs and equalized the sizes of EPSPs in a 20-Hz train...
  45. pmc The TTX-resistant sodium channel Nav1.8 (SNS/PN3): expression and correlation with membrane properties in rat nociceptive primary afferent neurons
    Laiche Djouhri
    Department of Physiology, University of Bristol Medical School, Bristol BS8 1TD, UK
    J Physiol 550:739-52. 2003
    ..Thus high levels of Nav1.8 protein may contribute to the longer AP durations (especially in A-fibre neurons) and larger AP overshoots that are typical of nociceptors...
  46. pmc Dynamic representation of whisker deflection by synaptic potentials in spiny stellate and pyramidal cells in the barrels and septa of layer 4 rat somatosensory cortex
    Michael Brecht
    Abteilung Zellphysiologie, Max Planck Institut fur medizinische Forschung, Heidelberg, Germany
    J Physiol 543:49-70. 2002
    ..This presumably reflects the divergence of thalamocortical projections to different barrels. Septum cell PSPs preferably represent multiple whisker deflections, but less dynamically and with less spatial acuity...
  47. ncbi Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice
    M Freichel
    Institut fur Pharmakologie und Toxikologie, Universitat des Saarlandes, D 66421 Homburg, Germany
    Nat Cell Biol 3:121-7. 2001
    ....
  48. ncbi Impaired thermosensation in mice lacking TRPV3, a heat and camphor sensor in the skin
    Aziz Moqrich
    Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 307:1468-72. 2005
    ..Camphor activated cultured primary keratinocytes but not sensory neurons, and this activity was abolished in TRPV3 null mice. Therefore, heat-activated receptors in keratinocytes are important for mammalian thermosensation...
  49. ncbi Differential roles of NR2A and NR2B-containing NMDA receptors in LTP and LTD in the CA1 region of two-week old rat hippocampus
    Thomas E Bartlett
    MRC Centre for Synaptic Plasticity, Department of Anatomy, University of Bristol, School of Medical Sciences, University Walk, Bristol, BS8 1TD, UK
    Neuropharmacology 52:60-70. 2007
    ..4 microM. The lack of effect of 0.1 microM NVP on LTD contrasts with its marked effect on LTP and raises the possibility that different NVP-sensitive NR2 subunit-containing NMDA receptors are required for LTP and LTD in this preparation...
  50. pmc TASK, a human background K+ channel to sense external pH variations near physiological pH
    F Duprat
    Institut de Pharmacologie Moleculaire et Cellulaire, CNRS, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France
    EMBO J 16:5464-71. 1997
    ..7 and only 10% at pH 6.7. This property is probably essential for its physiological function, and suggests that small pH variations may serve a communication role in the nervous system...
  51. pmc Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes
    G A Zampighi
    Department of Neurobiology, University of California, Los Angeles, School of Medicine, Los Angeles, California 90095, USA
    J Gen Physiol 113:507-24. 1999
    ..These exocytotic and endocytotic rates suggest that the entire plasma membrane of the oocyte is replaced in approximately 24 h...
  52. ncbi Modulation of triheteromeric NMDA receptors by N-terminal domain ligands
    Christopher J Hatton
    Laboratoire de Neurobiologie, CNRS UMR 8544, Ecole Normale Supérueure, Paris, France
    Neuron 46:261-74. 2005
    ..Similarly, NR1/2A/2C receptors are inhibited by zinc with high potency but low efficacy. Therefore, interactions between homologous N-terminal domains determine the unique pharmacological properties of triheteromeric NMDARs...
  53. ncbi Adenosine diphosphate as an intracellular regulator of insulin secretion
    C G Nichols
    Department of Cell Biology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Science 272:1785-7. 1996
    ..Thus, by binding to SUR NBF2 and antagonizing ATP inhibition of KATP++ channels, intracellular MgADP may regulate insulin secretion...
  54. ncbi Cellular expression and functional characterization of four hyperpolarization-activated pacemaker channels in cardiac and neuronal tissues
    S Moosmang
    , , Germany
    Eur J Biochem 268:1646-52. 2001
    ..Our results suggest that the heterogeneity of native I(h) currents is generated, at least in part, by the tissue-specific expression of HCN channel genes...
  55. ncbi Voltage-sensing mechanism is conserved among ion channels gated by opposite voltages
    Roope Mannikko
    Department of Neuroscience, The Nobel Institute for Neurophysiology, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    Nature 419:837-41. 2002
    ....
  56. ncbi Changes in the expression of tetrodotoxin-sensitive sodium channels within dorsal root ganglia neurons in inflammatory pain
    Joel A Black
    Department of Neurology and Paralyzes Veterans of America, Eastern Paralyzed Veterans Association Neuroscience Research Center, Yale University School of Medicine, New Haven CT 06510, USA
    Pain 108:237-47. 2004
    ....
  57. ncbi Contribution of sodium channel mutations to bradycardia and sinus node dysfunction in LQT3 families
    Marieke W Veldkamp
    Experimental and Molecular Cardiology Group, Department of Clinical and Experimental Cardiology, Academic Medical Center, University of Amsterdam, The Netherlands
    Circ Res 92:976-83. 2003
    ....
  58. ncbi State changes rapidly modulate cortical neuronal responsiveness
    Andrea Hasenstaub
    Department of Neurobiology, Kavli Institute of Neuroscience, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Neurosci 27:9607-22. 2007
    ..We conclude that stimulus-evoked or spontaneous alterations in cortical state can influence neuronal responsiveness in a complex manner, resulting in large changes in which, and how, sensory stimuli are represented...
  59. ncbi Relationship between membrane phosphatidylinositol-4,5-bisphosphate and receptor-mediated inhibition of native neuronal M channels
    Joanna S Winks
    1Ion Channel Pharmacology Group, IPC 388, Pfizer Global Research and Development, Sandwich Laboratories, Sandwich, Kent CT13 9NJ, United Kingdom
    J Neurosci 25:3400-13. 2005
    ....
  60. ncbi Regulation of synaptic efficacy by coincidence of postsynaptic APs and EPSPs
    H Markram
    Max Planck Institut fur medizinische Forschung, Abteilung Zellphysiologie, Jahnstrasse 29, D 69120 Heidelberg, Germany
    Science 275:213-5. 1997
    ..These observations suggest that APs propagating back into dendrites serve to modify single active synaptic connections, depending on the pattern of electrical activity in the pre- and postsynaptic neurons...
  61. pmc A yeast genetic screen reveals a critical role for the pore helix domain in TRP channel gating
    Benjamin R Myers
    Department of Physiology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94143 2140, USA
    Neuron 58:362-73. 2008
    ..Our data suggest that conformational changes in the outer pore region are critical for determining the balance between open and closed states, providing evidence for a general role for this domain in TRP channel activation...
  62. ncbi Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain
    B Santoro
    Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA
    Cell 93:717-29. 1998
    ....
  63. pmc Mutations of the S4-S5 linker alter activation properties of HERG potassium channels expressed in Xenopus oocytes
    M C Sanguinetti
    Eccles Program in Human Molecular Biology and Genetics, Division of Cardiology, Department of Medicine, University of Utah, Salt Lake City, UT 84112
    J Physiol 514:667-75. 1999
    ..5. The results indicate that the S4-S5 linker is a crucial component of the activation gate of HERG channels...
  64. ncbi Dopamine modulation of state-dependent endocannabinoid release and long-term depression in the striatum
    Anatol C Kreitzer
    Department of Psychiatry and Behavioral Sciences, Stanford University Medical School, Palo Alto, California 94305, USA
    J Neurosci 25:10537-45. 2005
    ..However, this gating of plasticity by dopamine is accomplished via an unexpected mechanism involving the regulation of mGluR-dependent endocannabinoid release...
  65. ncbi Diverse types of interneurons generate thalamus-evoked feedforward inhibition in the mouse barrel cortex
    J T Porter
    Department of Neurobiology and Anatomy and the Sensory Neuroscience Research Center, West Virginia University, Morgantown, West Virginia 26506-9128, USA
    J Neurosci 21:2699-710. 2001
    ..We conclude that feedforward inhibition is generated by diverse classes of interneurons, possibly serving different roles in the processing of incoming sensory information...
  66. ncbi The acid-activated ion channel ASIC contributes to synaptic plasticity, learning, and memory
    John A Wemmie
    Department of Psychiatry, University of Iowa, Iowa City, IA 52242, USA
    Neuron 34:463-77. 2002
    ..These results identify ASIC as a key component of acid-activated currents and implicate these currents in processes underlying synaptic plasticity, learning, and memory...
  67. pmc Electrophysiological and neuroanatomical evidence of sexual dimorphism in aortic baroreceptor and vagal afferents in rat
    Bai Yan Li
    Department of Pharmacology, Harbin Medical University, Harbin, China
    Am J Physiol Regul Integr Comp Physiol 295:R1301-10. 2008
    ..Possible explanations are presented as to how such a greater prevalence of low-threshold myelinated afferents could be a contributing factor to the altered baroreflex sensitivity and vagal tone of females compared with males...
  68. pmc Acid-sensing ion channel 3 matches the acid-gated current in cardiac ischemia-sensing neurons
    S P Sutherland
    The Vollum Institute, Oregon Health Sciences University, Portland, OR 97201 3098, USA
    Proc Natl Acad Sci U S A 98:711-6. 2001
    ....
  69. ncbi Ionic mechanisms of electrical remodeling in human atrial fibrillation
    R F Bosch
    Department of Cardiology, University of Tubingen, Germany
    Cardiovasc Res 44:121-31. 1999
    ..The cellular changes in repolarization and the underlying ionic mechanisms in human AF are only poorly understood...
  70. pmc Roles of the AMPA receptor subunit GluA1 but not GluA2 in synaptic potentiation and activation of ERK in the anterior cingulate cortex
    Hiroki Toyoda
    Department of Physiology, Faculty of Medicine, University of Toronto, University of Toronto Centre for the Study of Pain, 1 King s College Circle, Ontario, Canada
    Mol Pain 5:46. 2009
    ..Our results demonstrate that AMPA receptor subunit GluA1 is a key mechanism for the expression of ACC LTP and inflammation-induced long-term plastic changes in the ACC...
  71. ncbi The principle of temperature-dependent gating in cold- and heat-sensitive TRP channels
    Thomas Voets
    Laboratory of Physiology, Campus Gasthuisberg, KU Leuven, B 3000 Leuven, Belgium
    Nature 430:748-54. 2004
    ..Our results suggest a simple unifying principle that explains both cold and heat sensitivity in TRP channels...
  72. ncbi Activation of NR2A-containing NMDA receptors is not obligatory for NMDA receptor-dependent long-term potentiation
    Carl Weitlauf
    Center for Molecular Neuroscience, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0615, USA
    J Neurosci 25:8386-90. 2005
    ..Furthermore, our data demonstrate that care must be exercised in the interpretation of data generated with AAM077 when the compound is applied before an agonist...
  73. ncbi Identification of receptors of main sex-pheromone components of three Lepidopteran species
    Hidefumi Mitsuno
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Eur J Neurosci 28:893-902. 2008
    ....
  74. ncbi Roles of tetrodotoxin (TTX)-sensitive Na+ current, TTX-resistant Na+ current, and Ca2+ current in the action potentials of nociceptive sensory neurons
    Nathaniel T Blair
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 20114, USA
    J Neurosci 22:10277-90. 2002
    ....
  75. ncbi CaT1 and the calcium release-activated calcium channel manifest distinct pore properties
    T Voets
    Laboratory of Physiology, Catholic University of Leuven, B 3000 Leuven, Belgium
    J Biol Chem 276:47767-70. 2001
    ..Our results imply that the pores of CaT1 and CRAC are not identical and indicate that CaT1 is a Mg(2+)-gated channel not directly related to CRAC...
  76. pmc Synaptic background noise controls the input/output characteristics of single cells in an in vitro model of in vivo activity
    J M Fellous
    Computational Neurobiology Laboratory, Howard Hughes Medical Institute, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Neuroscience 122:811-29. 2003
    ..We conclude that background synaptic activity can dynamically modulate the input/output properties of individual neocortical neurons in vivo...
  77. ncbi Barrel cortex microcircuits: thalamocortical feedforward inhibition in spiny stellate cells is mediated by a small number of fast-spiking interneurons
    Qian Quan Sun
    Department of Neurology and Neurological Sciences, Stanford School of Medicine, Stanford, California 94305, USA
    J Neurosci 26:1219-30. 2006
    ..The ready activation of FS cells by TC inputs, coupled with powerful feedforward inhibition from these neurons, would profoundly influence sensory processing and constrain runaway excitation in vivo...
  78. ncbi Induction of synaptic long-term potentiation after opioid withdrawal
    Ruth Drdla
    Department of Neurophysiology, Center for Brain Research, Medical University of Vienna, Spitalgasse 4, 1090 Vienna, Austria
    Science 325:207-10. 2009
    ..These findings provide a previously unrecognized target to selectively combat pro-nociceptive effects of opioids without compromising opioid analgesia...
  79. pmc Energetic and spatial parameters for gating of the bacterial large conductance mechanosensitive channel, MscL
    S I Sukharev
    Department of Biology, University of Maryland, College Park, Maryland 20742, USA
    J Gen Physiol 113:525-40. 1999
    ..The higher conducting states appear to involve conformational changes internal to the channel that don't involve changes in area...
  80. pmc Hysteresis in the voltage dependence of HCN channels: conversion between two modes affects pacemaker properties
    Roope Mannikko
    Department of Neuroscience, The Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden
    J Gen Physiol 125:305-26. 2005
    ..Computer simulations suggest that voltage hysteresis in HCN channels decreases the risk of arrhythmia in pacemaker cells...
  81. pmc GTP up-regulated persistent Na+ current and enhanced nociceptor excitability require NaV1.9
    Johan A R Ostman
    Centre for Neuroscience, Institute of Cell and Molecular Science, Queen Mary University of London, Newark Street, London E1 2AT, UK
    J Physiol 586:1077-87. 2008
    ..9 underlies the G-protein pathway-regulated TTX-r persistent Na(+) current in small diameter sensory neurones that may drive spontaneous discharge in nociceptive nerve fibres during inflammation...
  82. pmc BK channels are linked to inositol 1,4,5-triphosphate receptors via lipid rafts: a novel mechanism for coupling [Ca(2+)](i) to ion channel activation
    Amy K Weaver
    Department of Neurobiology, Center for Glial Biology in Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 282:31558-68. 2007
    ..Taken together, these data suggest a privileged association of BK channels in lipid raft domains and provide evidence for a novel coupling of these Ca(2+)-sensitive channels to their second messenger source...
  83. ncbi Targeted transgene expression in neuronal precursors: watching young neurons in the old brain
    Sebastien Couillard-Despres
    Volkswagen Foundation Junior Group, University of Regensburg, Franz Josef Strauss Allee 11, 93053 Regensburg, Germany
    Eur J Neurosci 24:1535-45. 2006
    ..Therefore, maintenance of the fundamental properties of neuronal precursors even at advanced age suggests that stimulation of neurogenesis may constitute a valid strategy to counteract age-related neuronal loss and cognitive declines...
  84. pmc Electrophysiological analysis of cloned cyclic nucleotide-gated ion channels
    Qiang Leng
    Department of Plant Science, University of Connecticut, Storrs, CT 06269 4067, USA
    Plant Physiol 128:400-10. 2002
    ..Addition of a lipophilic analog of cAMP to the perfusion bath of oocytes injected with NtCBP4 and AtCNGC1 cRNAs induced inward rectified, noninactivating K(+) currents...
  85. ncbi Identified sources and targets of slow inhibition in the neocortex
    Gabor Tamas
    Department of Comparative Physiology, University of Szeged, Közép fasor 52, Szeged H 6726, Hungary
    Science 299:1902-5. 2003
    ..Slow inhibition evoked by a distinct interneuron in spatially restricted postsynaptic compartments could locally and selectively modulate cortical excitability...
  86. pmc Long-term potentiation in rat hippocampal neurons is accompanied by spatially widespread changes in intrinsic oscillatory dynamics and excitability
    Rishikesh Narayanan
    Center for Learning and Memory, The University of Texas at Austin, Austin, TX 78712, USA
    Neuron 56:1061-75. 2007
    ..Our results suggest that the frequency response of these neurons depends on the dendritic location of their inputs and that activity can regulate their response dynamics within an oscillating neural network...
  87. ncbi Role of NMDA receptor subtypes in governing the direction of hippocampal synaptic plasticity
    Lidong Liu
    Brain Research Centre, University of British Columbia, 2211 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada
    Science 304:1021-4. 2004
    ..These results demonstrate that distinct NMDAR subunits are critical factors that determine the polarity of synaptic plasticity...
  88. ncbi A simple technique for isolating healthy heart cells from mouse models
    Takao Shioya
    Department of Physiology, Faculty of Medicine, Saga University, Saga 849 8501, Japan
    J Physiol Sci 57:327-35. 2007
    ..The beta-adrenergic signal transduction of the cells was also normal when it was examined with the isoproterenol enhancement of the L-type Ca2+ current...
  89. ncbi Morpho-functional characterization of neuronal cells at different stages of maturation in granule cell layer of adult rat dentate gyrus
    Patrizia Ambrogini
    Istituto di Scienze Fisiologiche, Universita degli Studi di Urbino, Carlo Bo, Crocicchia, I 61029 Urbino, Italy
    Brain Res 1017:21-31. 2004
    ..Finally, cells showing electrophysiological, synaptic, and morphological features of mature granule, expressing the mature neuron marker NeuN, were identified...
  90. ncbi Intrinsic mechanisms for adaptive gain rescaling in barrel cortex
    Marta Díaz-Quesada
    Instituto de Neurociencias de Alicante, Universidad Miguel Hernández de Elche Consejo Superior de Investigaciones Científicas, Campus de San Juan, 03550 Sant Joan d Alacant, Spain
    J Neurosci 28:696-710. 2008
    ..Our results suggest that widely expressed intrinsic mechanisms participate in barrel cortex adaptation but that their recruitment is highly stimulus specific...
  91. pmc Functional ion channels in pulmonary alveolar type I cells support a role for type I cells in lung ion transport
    Meshell D Johnson
    Department of Medicine, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 103:4964-9. 2006
    ....
  92. pmc Maturation of intrinsic and synaptic properties of layer 2/3 pyramidal neurons in mouse auditory cortex
    Anne Marie M Oswald
    Center for Neural Science, New York University, 4 Washington Place, Rm 809, New York, NY 10003, USA
    J Neurophysiol 99:2998-3008. 2008
    ..We show that these properties combine to produce age-related differential synaptic responses to low- and high-frequency synaptic input that may contribute to differences in auditory processing during development...
  93. ncbi Lipid binding regulates synaptic targeting of PICK1, AMPA receptor trafficking, and synaptic plasticity
    Wenying Jin
    Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Neurosci 26:2380-90. 2006
    ..Together, our findings indicate that the lipid binding of the PICK1 BAR domain is important for its synaptic targeting, AMPA receptor trafficking, and synaptic plasticity...
  94. pmc Morphology of inhibitory and excitatory interneurons in superficial laminae of the rat dorsal horn
    David J Maxwell
    Spinal Cord Group, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    J Physiol 584:521-33. 2007
    ..Our hypothesis appears to be correct only for islet cells. Other classes of cells have mixed actions, and in the case of vertical cells, the axonal projection appears to be a more important determinant of postsynaptic action...
  95. ncbi Assaying phosphatidylinositol bisphosphate regulation of potassium channels
    Tibor Rohacs
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Methods Enzymol 345:71-92. 2002
  96. ncbi Endogenous voltage-gated potassium channels in human embryonic kidney (HEK293) cells
    S P Yu
    Department of Neurology, Center for the Study of Nervous System Injury, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci Res 52:612-7. 1998
    ..The difference in endogenous voltage-activated currents in HEK293 and CHO cells suggest that CHO cell lines are a preferred system for exogenous K+ channel expression...
  97. ncbi Essential Ca(2+)-binding motif for Ca(2+)-sensitive inactivation of L-type Ca2+ channels
    M De Leon
    Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 270:1502-6. 1995
    ..Donation of the alpha 1C EF-hand region to the alpha 1E channel conferred the Ca(2+)-inactivating phenotype. These results strongly suggest that Ca2+ binding to the alpha 1C subunit initiates Ca2+ inactivation...
  98. pmc Early intermediates in the transport cycle of the neuronal excitatory amino acid carrier EAAC1
    N Watzke
    , D-60596 Frankfurt, Germany
    J Gen Physiol 117:547-62. 2001
    ..Once the fully loaded EAAC1 complex is formed, it can undergo a much slower, electrogenic translocation reaction to expose the substrate and ion binding sites to the cytoplasm...
  99. ncbi Vesicular neurotransmitter transporter expression in developing postnatal rodent retina: GABA and glycine precede glutamate
    Juliette Johnson
    Department of Ophthalmology, University of California School of Medicine, San Francisco, California 94143 0730, USA
    J Neurosci 23:518-29. 2003
    ..Furthermore, vesicular glutamate transmission likely occurs before ribbon synapse formation in the inner retina...
  100. ncbi NMDA receptors in layer 4 spiny stellate cells of the mouse barrel cortex contain the NR2C subunit
    Alexander M Binshtok
    Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot 76100, Israel
    J Neurosci 26:708-15. 2006
    ....
  101. ncbi Functional roles of charged residues in the putative voltage sensor of the HCN2 pacemaker channel
    J Chen
    Department of Medicine, Division of Cardiology and Eccles Program in Human Molecular Biology and Genetics, University of Utah, Salt Lake City, Utah 84112 5330, USA
    J Biol Chem 275:36465-71. 2000
    ....

Research Grants63

  1. Kainate Receptors on Hippocampal Interneurons
    Matthew Frerking; Fiscal Year: 2007
    ..Using whole-cell patch clamp techniques, the activity of KARs on interneurons will be recorded and manipulated using pharmacological tools...
  2. ALCOHOL MODULATION OF CARDIAC CALCIUM CHANNELS
    Gary Aistrup; Fiscal Year: 2003
    ..The overall objective of the proposed studies is to use whole-cell patch clamp techniques to analyze ethanol modulation of cardiac calcium channels...
  3. MECHANISMS OF CARDIOTOXICITY OF ANTIPSYCHOTIC DRUGS
    David Flockhart; Fiscal Year: 2001
    ..Na, Ca, or K, channel activity in isolated ventricular cardiomyocytes and Purkinje cells, using currient patch clamp techniques. Since the investigators have preliminary data that indicate that haloperidol can slow cardiac ..
  4. Ionic currents in gastrointestinal smooth muscle
    Hamid I Akbarali; Fiscal Year: 2013
    ..phosphorylation and nitration on voltage-dependent inactivation using single channel and whole-cell patch clamp techniques. We will also investigate the interaction of src kinase with the calcium channel using fluorescence ..
  5. Characterization of Estrogen Sensitive Hypothalamic Glucose Sensing Neurons
    AMMY MARIE SANTIAGO; Fiscal Year: 2013
    ....
  6. MECHANOTRANSDUCTION IN INTESTINAL SMOOTH MUSCLE CELLS
    Gianrico Farrugia; Fiscal Year: 2013
    ..5 sets local intracellular Na+ and regulates Ca2+ through Na+/Ca2+ exchanger (NCX). We will use patch clamp techniques, ultrafast pressure delivery, high resolution patch imaging, immunohistochemistry, Western blots, single ..
  7. Ryanodine receptor in cardiac hypertrophy and heart failure
    GERHARD W MEISSNER; Fiscal Year: 2013
    ..Ca2+ handling by wild type and mutant mice will be assessed using whole cell patch clamp techniques, Ca2+ imaging, cell homogenates and membrane preparations...
  8. Control of KATP channel expression by CaMKII: role in heart failure resistance
    DENICE HODGSON-ZINGMAN; Fiscal Year: 2013
    ..tagged recombinant KATP channel subunits, confocal immunofluorescence imaging, and molecular biology and patch clamp techniques in cardiomyocytes and HEK293T cells...
  9. Estrogen and EDHF in NO Deficiency
    An Huang; Fiscal Year: 2012
    ..The knowledge generated by these studies will unravel a novel therapeutic target to improve vascular endothelial function by the upregulation of CYP-dependent dilator pathways as an adaptation to NO deficiency. ..
  10. The Effects of CFTR Dysfunction on Bone Formation
    Marie E Egan; Fiscal Year: 2012
    ..By employing in situ hybridization, RT-PCR, and immunohistochemistry as well as standard patch clamp techniques, the functional role of CFTR in osteoblasts and osteoclasts will be examined...
  11. Glutamate Mediates Platelet Activation through an Ionotropic Glutamate Receptor
    Craig N Morrell; Fiscal Year: 2012
    ..Using flow cytometry, platelet aggregation, and whole cell patch clamp techniques we will further dissect platelet glutamate signaling...
  12. Cholinergic receptors on the nematode pharynx: an unexploited drug target
    Alan P Robertson; Fiscal Year: 2012
    ..The longer term goal of this research is provide ion- channel drug targets from a range of important parasite species in a platform (Xenopus oocytes) suitable for drug discovery screening. ..
  13. CONTROL OF NEONATAL CIRCULATION
    Charles W Leffler; Fiscal Year: 2013
    ..b>Patch clamp techniques will be used to examine BKCa channel activity...
  14. Diet-Induced Plasticity of Proopiomelanocortin Neurons
    AARON JEFFREY MERCER; Fiscal Year: 2012
    ..To directly examine POMC morphology and physiology at the cellular level, we will use single cell patch clamp techniques to measure basal neural activity and simultaneously label cells with neurobiotin...
  15. Regulation of Vascular BK Channel in Diabetes
    Hon Chi Lee; Fiscal Year: 2013
    ..Whole-cell and single channel patch clamp techniques, antioxidant enzyme in vitro gene transfer, and specific transgenic mice will be employed to determine the ..
  16. Mitochondrial ion channels in hypoxic neurons
    Elizabeth Ann Jonas; Fiscal Year: 2013
    ..We have developed patch clamp techniques to record mitochondrial ion channel activity within living neurons and to study the ion channel activity ..
  17. Regulation of voltage-gated calcium channels during chronic BZ treatment in rats
    DAMIEN EUGENE EARL; Fiscal Year: 2013
    ..Direct inhibition of recombinant L-VGCCs by BZs will be measured using whole-cell patch clamp techniques. Chronic misuse of prescription benzodiazepines (BZs) can result in physical dependence and remains a ..
  18. CELLULAR AND MOLECULAR STUDIES OF RENAL TRANSPORT
    Michael J Caplan; Fiscal Year: 2012
    ..Our strategy to pursue these themes successfully will include close collaboration on interrelated research projects;sharing of expertise, concepts and techniques by Program investigators;and joint use of core facilities. ..
  19. Na/Bicarbonate Cotransporters in Brain
    MARK OLIVER BEVENSEE; Fiscal Year: 2010
    ..The information \,;;1\ enhance our understa nding of acid -base handling by brain cells, partieul arly in acid-base disturbances associ:Jted \,;;th ischemia, anoxia/ hypoxia, stroh, and reperftlsion inj ury. ..
  20. Exploring Vasomotor Mechanisms using New PKG Inhibitors and cGMP Biosensors
    Wolfgang R Dostmann; Fiscal Year: 2012
    ..be multidisciplinary, employing state-of-the-art techniques from physiology (high speed calcium imaging, patch clamp techniques, resistance artery myography, blood pressure and blood flow measurements), cell biology (confocal ..
  21. Integration of Peripheral and Central Appetite Signals by Brainstem Neurons
    Suzanne M Appleyard; Fiscal Year: 2013
    ..stimulate visceral afferents while recording from identified NTS-CA neurons using electrophysiological patch clamp techniques. This is an extremely powerful system to address the cellular and molecular mechanisms by which NTS-CA ..
  22. Sodium Channel Gene Variation in the Treatment of Epilepsy
    Katherine D Holland; Fiscal Year: 2013
    ..the functional consequences of these sodium channel SNPs will be studied in vitro with whole cell patch clamp techniques using heterologously expressed recombinant human sodium channels...
  23. General Anesthetic Sites on Ligand-Gated Ion Channels
    Keith W Miller; Fiscal Year: 2013
    ..and (ii) define how their occupancy allosterically modulates receptor function using rapid perfusion patch clamp techniques. Project 1 will locate sites of etomidate, propofol and barbiturate photolabels on equilibrium states of ..
  24. Novel signaling between striatal neurons and postnatal neural progenitors
    Stephanie Young; Fiscal Year: 2010
    ..to look at the interaction between striatal neurons and SVZ cells using dye-filling, calcium imaging, and patch clamp techniques. Aim 1 proposes to determine the presence of striatal neuron processes in the SVZ and characterize ..
  25. Comparison between pH regulation in chemosensitive vs nonchemosensitive neurons
    VERNON A RUFFIN; Fiscal Year: 2010
    ..Aim 3. To determine if pH-chemosensitivity in MR neurons correlates with pHi physiology. I will use patch clamp techniques. (A) Does the ability of MR neurons to alter firing rate in response to MAc correlate with MAc sensitivity ..
  26. REGULATION OF SYNAPTIC AMINO ACID RECEPTORS
    Laurence O Trussell; Fiscal Year: 2010
    ..Moreover, this work may lend new clues to the treatment of tinnitus, a condition thought to be maintained by long-term plasticity in this brain region. ..
  27. CALCIUM DYNAMICS IN INTERSTITIAL CELLS OF CAJAL
    Gianrico Farrugia; Fiscal Year: 2013
    ..bioinformatic analysis of microarray data, proteomics, Ca2+ and Cl- imaging, microelectrode recordings and patch clamp techniques. Successful completion of the proposed studies has both basic significance and clinical impact...
  28. Effects of TrkB Activation on Abnormalities in Neocortical FS Interneurons
    DAVID ALLAN PRINCE; Fiscal Year: 2013
    ..After treatment for ~2 weeks, rodents are re-anesthetized, and standard in vitro slice and patch clamp techniques used to obtain recordings from single interneurons and excitatory cells in areas of injury from drug-..
  29. FASEB SRC on Ion Channel Regulation
    Geoffrey W Abbott; Fiscal Year: 2013
    ..The conference venue has appropriate facilities for disabled attendees. ..
  30. A custom microchip amplifier for patch clamp electrophysiological recording
    Reid Harrison; Fiscal Year: 2013
    ..As a result of this innovation, patch clamp techniques are being adapted to a wider variety of experimental protocols and target species, and researchers are now ..
  31. LOW LEVEL HYPERBARIC ETHANOL ANTAGONISM
    RONALD ALKANA; Fiscal Year: 2009
    ..of mutant receptor properties will be conducted in HEK 293 and/or NG 108 cells using whole cell and patch clamp techniques. Aim 1 - Use pressure and molecular manipulations to identify and test novel sites of action for ethanol ..
  32. Abnormalities in Fast - Spiking Interneurons of Chronically Epileptogenic Cortex
    DAVID ALLAN PRINCE; Fiscal Year: 2011
    ..In vitro slices from chronically injured epileptogenic cortex together with patch clamp techniques and dual recordings will be used to assess the functional disorders in inhibition that occur as a result ..
  33. Molecular Mechanisms of Ion Channels in T Lymphocytes
    Michael D Cahalan; Fiscal Year: 2012
    ..abstract_text> ..
  34. Reversal of pain by group II metabotropic glutamate receptors
    Steve Davidson; Fiscal Year: 2013
    ..Finally, we will determine whether group II mGluRs are capable of relieving ongoing neuropathic pain using an operant conditioning paradigm. ..
  35. Functions of naturally diverse inhibitory networks in neocortex
    Shane R Crandall; Fiscal Year: 2013
    ..To address these aims I will use a combination of in vitro patch clamp techniques, various optogenetic approaches, quantitative histological methods, and multiple transgenic lines of mice ..
  36. GATING AND TRANSLOCATION IN THE DIPHTHERIA TOXIN CHANNEL
    Bruce Kagan; Fiscal Year: 1992
    ..Channels will be studied using lipid bilayer, lipid vesicle, and patch clamp techniques. 1. Pharmacology of DT gating...
  37. Functional role of fibroblast-like cells in GI muscles
    Kenton M Sanders; Fiscal Year: 2013
    ..We will isolate the PDGFR-alpha+ cells and use patch clamp techniques to study the endogenous conductances expressed by these cells and characterize the effects of enteric ..
  38. Cell-type Specificity of Heritable Channelopathies: Role of the Purkinje Fiber
    Vivek Iyer; Fiscal Year: 2013
    ..training in ion channel biophysics and the mathematical sciences and further my technical expertise with patch clamp techniques and in vitro analysis of cellular electrophysiology...
  39. DOPAMINERGIC AND MUSCARINIC SIGNALING IN THE STRIATUM
    DALTON JAMES SURMEIER; Fiscal Year: 2013
    ..To overcome the inaccessibility of MSN dendrites to conventional patch clamp techniques, we have gained expertise in two- photon laser scanning microscopy (2PLSM) and 2P laser uncaging (2PLU), ..
  40. The effects of alcohol on cerebellar synaotic transmission and plasticity
    CHRISTIAN ROBERT HANSEL; Fiscal Year: 2010
    ....
  41. Neurotransmission at the vestibular calyx synapse
    KATHERINE RENNIE; Fiscal Year: 2009
    ..In Aim 1, patch clamp techniques will be used to study ionic conductances &transmitter release in a newly developed preparation of ..
  42. NEUROMODULATION BY ZINC IN THE HIPPOCAMPAL REGION
    John White; Fiscal Year: 2000
    ..Examples of brain disorders to which zinc metabolism in the hippocampal region has been linked include Alzheimer's disease, temporal lobe epilepsy, and stroke-related cell death. ..
  43. G PROTEIN-MEDIATED K+ CHANNEL ACTIVATION IN HEART
    GERDA BREITWIESER; Fiscal Year: 1993
    ..hypotheses will be tested, in experiments which will utilize both the whole cell and single channel patch clamp techniques. A key question is whether any of the characteristic kinetic properties ascribed to the potassium channel ..
  44. DEVELOPMENT AND MODULATION OF PRESYNAPTIC ION CHANNELS
    STEPHEN MERINEY; Fiscal Year: 2000
    ..Using patch clamp techniques, I propose to (1) characterize directly the types of currents present at newly formed presynaptic ..
  45. PHARMACOLOGICAL STUDIES OF CENTRAL SYNAPTIC TRANSMISSION
    SOLOMON ERULKAR; Fiscal Year: 1990
    ..By the use of patch clamp techniques, we have shown that 5-alpha-dihydrotestosterone (5 alpha-DHT) added to the bath can induce: 1) "anesthetic-..
  46. HYPERTENSION MECHANISMS AND VASCULAR ION EXCHANGE
    Allan Jones; Fiscal Year: 2000
    ..Moreover, this model which appears to be critically dependent on SBB will allow us to test concepts that have wide applicability to other cell systems which have significant interactions between plasma and SR membranes. ..
  47. CELLULAR CONTROL MECHANISMS OF CEREBROVASCULAR TONE
    David Harder; Fiscal Year: 1999
    ..arterial to examine the effect of endothelial factors on whole cell K+ and Ca2+ current measured via patch clamp techniques. Microsomal fractions of arteries loaded with radiolabeled arachidonic acid produce a HPLC fraction which ..
  48. HORMONAL INFLUENCES ON THE RENAL MICROVASCULATURE
    PAMELA CARMINES; Fiscal Year: 2002
    ..isolated from the rat preglomerular microvasculature will be used, together with fluorescent probes and patch clamp techniques, in studies designed to determine the impact of Ca2+-activated K+ channels and Ca2+- activated CI channels ..
  49. STIMULUS-SECRETION COUPLING IN MAST CELLS
    JULIO FERNANDEZ; Fiscal Year: 1991
    ..These results will help understanding the mechanisms of Antigen induced release of inflammatory mediators as well as the mechanism of exocytosis in general...
  50. Subconductance behavior in T-type calcium channels
    KATIE BITTNER; Fiscal Year: 2009
    ..Specifically subconductance behavior in these channels will be examined using single channel patch clamp techniques to provide a quantitative measure of Ca flux through these channels...
  51. SODIUM CHANNELS IN COLONIC EPITHELIAL CELLS
    LAWRENCE PALMER; Fiscal Year: 1993
    ..These studies will give insights into the basic mechanisms of colonic ion transport and its regulation, and will help in understanding diseases in which epithelial transport is deranged such as diarrhea and cystic fibrosis...
  52. EARLY RECEPTOR CURRENT OF RHODOPSIN ACTIVATION
    John Sullivan; Fiscal Year: 2000
    ..the conformation-dependent charge flow in rhodopsin during activation, can be studied using whole-cell patch clamp techniques and is the fundamental process underlying the familiar early receptor potential (ERP)...
  53. AMINO ACID RECEPTORS IN VERTEBRATE SPINAL NEURONS
    James Buchanan; Fiscal Year: 1993
    ..These studies will contribute to our basic understanding of synaptic function in the spinal cord, which ultimately will be important in the development of therapeutic measures to treat spinal cord injury and disease...
  54. ION CHANNELS AND PULMONARY VASCULAR TONE
    JASON YUAN; Fiscal Year: 1999
    ..I propose to investigate the role of ionic channels in HPV by using patch clamp techniques and fluorescent imaging microscopy in order to identify the critical pathways leading to HPV...
  55. ETHANOL GABA INTERACTIONS IN MAMMALIAN CULTURED NEURONS
    Maharaj Ticku; Fiscal Year: 1999
    ..This will be achieved by using a comprehensive approach including radioligand binding, 36Cl- influx, patch clamp techniques, measurement of GABA-A receptor subunit polypeptide levels by Western blot and immunoprecipitation studies...
  56. Molecular Mechanism Underlying Alcohol Modulation of Inward Rectifying K+ Channel
    Prafulla Aryal; Fiscal Year: 2009
    ..This study could also lead to development of pharmaceutical agents designed to prevent or treat addiction and abuse associated with consumption of alcohol. ..
  57. MEMBRANE EXCITABILITY AND SECRETION FROM NERVE ENDINGS
    Meyer Jackson; Fiscal Year: 2000
    DESCRIPTION: Patch clamp techniques will be used to investigate the regulation of secretion from nerve terminals of the posterior pituitary (neurohypophysis)...
  58. OPIOID AND G-PROTEIN REGULATION OF CALCIUM CURRENTS
    HYLAN MOISES; Fiscal Year: 2001
    ..The experiments will utilize whole-cell patch clamp techniques to record Ca++ currents in combination with monitoring of exocytosis using time-resolved measurements of ..
  59. REGULATION OF NA+ & K+ TRANSPORT ACROSS EPITHELIA
    NANCY WILLS; Fiscal Year: 1992
    ..and ion-sensitive microelectrode methods, impedance analysis, current fluctuation analysis and patch clamp techniques will be used...
  60. Prevention of Postraumatic Neocortical Epileptogenesis
    Kevin Graber; Fiscal Year: 2005
    ..b>Patch clamp techniques will be used to evaluate miniature, spontaneous and evoked inhibitory currents in pyramidal neurons in ..
  61. CARDIAC CA CHANNELS--REGULATION OF SLOW GATING BEHAVIOR
    David Yue; Fiscal Year: 1993
    ..The overall goal of this project is to use patch clamp techniques to establish a rigorous understanding, at the single channel and molecular level, of the slow transitions ..
  62. REGULATION OF SODIUM TRANSPORT IN TIGHT EPITHELIA
    Douglas Eaton; Fiscal Year: 2001
    ..Therefore, this application proposes to investigates, using patch clamp techniques supplemented by direct biochemical measurements, the signaling cascades which regulate sodium channels in ..
  63. NEUROTRANSMITTER CONTROL OF IONIC CHANNELS
    BRUCE BEAN; Fiscal Year: 2001
    ..b>Patch clamp techniques will be used to study the control of calcium channels in cardiac myocytes by beta-adrenergic stimulation, ..