paraffin embedding

Summary

Summary: The infiltrating of tissue specimens with paraffin, as a supporting substance, to prepare for sectioning with a microtome.

Top Publications

  1. pmc Gene expression in fixed tissues and outcome in hepatocellular carcinoma
    Yujin Hoshida
    Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02142, USA
    N Engl J Med 359:1995-2004. 2008
  2. ncbi Quantitative determination of estrogen receptor, progesterone receptor, and HER2 mRNA in formalin-fixed paraffin-embedded tissue--a new option for predictive biomarker assessment in breast cancer
    Berit Maria Müller
    Institute of Pathology, Charite Hospital, Campus Mitte, Berlin, Germany
    Diagn Mol Pathol 20:1-10. 2011
  3. pmc Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples
    Craig April
    Illumina, Inc, San Diego, California, United States of America
    PLoS ONE 4:e8162. 2009
  4. pmc The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?
    M Thomas P Gilbert
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona, United States of America
    PLoS ONE 2:e537. 2007
  5. ncbi Robust global micro-RNA profiling with formalin-fixed paraffin-embedded breast cancer tissues
    Angela B Y Hui
    Division of Applied Molecular Oncology, Ontario Cancer Institute, University Health Network, Toronto, ON, Canada
    Lab Invest 89:597-606. 2009
  6. pmc Determinants of RNA quality from FFPE samples
    Silke von Ahlfen
    QIAGEN GmbH, Hilden, Germany
    PLoS ONE 2:e1261. 2007
  7. pmc Gene expression profiles from formalin fixed paraffin embedded breast cancer tissue are largely comparable to fresh frozen matched tissue
    Lorenza Mittempergher
    Divisions of Experimental Therapy, Molecular Biology, Surgical Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS ONE 6:e17163. 2011
  8. ncbi Automated extraction of DNA and RNA from a single formalin-fixed paraffin-embedded tissue section for analysis of both single-nucleotide polymorphisms and mRNA expression
    Guido Hennig
    Siemens Healthcare Diagnostics Products, Molecular Research Germany, Cologne, Germany
    Clin Chem 56:1845-53. 2010
  9. ncbi Cross-validation study for epidermal growth factor receptor and KRAS mutation detection in 74 blinded non-small cell lung carcinoma samples: a total of 5550 exons sequenced by 15 molecular French laboratories (evaluation of the EGFR mutation status for th
    Michèle Beau-Faller
    Laboratoire de Biologie Moleculaire, Hopital de Hautepierre, Strasbourg, France
    J Thorac Oncol 6:1006-15. 2011
  10. pmc Histological assessment of PAXgene tissue fixation and stabilization reagents
    Marcel Kap
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC, Rotterdam, The Netherlands
    PLoS ONE 6:e27704. 2011

Detail Information

Publications256 found, 100 shown here

  1. pmc Gene expression in fixed tissues and outcome in hepatocellular carcinoma
    Yujin Hoshida
    Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02142, USA
    N Engl J Med 359:1995-2004. 2008
    ..An obstacle to the development of genome-based predictors of outcome in patients with hepatocellular carcinoma has been the lack of a means to carry out genomewide expression profiling of fixed, as opposed to frozen, tissue...
  2. ncbi Quantitative determination of estrogen receptor, progesterone receptor, and HER2 mRNA in formalin-fixed paraffin-embedded tissue--a new option for predictive biomarker assessment in breast cancer
    Berit Maria Müller
    Institute of Pathology, Charite Hospital, Campus Mitte, Berlin, Germany
    Diagn Mol Pathol 20:1-10. 2011
    ..For the assessment of ESR1, PGR, and HER2 the results are comparable to the current gold-standard...
  3. pmc Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples
    Craig April
    Illumina, Inc, San Diego, California, United States of America
    PLoS ONE 4:e8162. 2009
    ..We have developed a gene expression assay (Whole-Genome DASL), capable of generating whole-genome gene expression profiles from degraded samples such as formalin-fixed, paraffin-embedded (FFPE) specimens...
  4. pmc The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?
    M Thomas P Gilbert
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona, United States of America
    PLoS ONE 2:e537. 2007
    ..Our findings recommend that the extraction methodology adopted should be chosen carefully. Here we provide a quick reference table that can be used to determine appropriate protocols for particular aims...
  5. ncbi Robust global micro-RNA profiling with formalin-fixed paraffin-embedded breast cancer tissues
    Angela B Y Hui
    Division of Applied Molecular Oncology, Ontario Cancer Institute, University Health Network, Toronto, ON, Canada
    Lab Invest 89:597-606. 2009
    ....
  6. pmc Determinants of RNA quality from FFPE samples
    Silke von Ahlfen
    QIAGEN GmbH, Hilden, Germany
    PLoS ONE 2:e1261. 2007
    ..The results are particularly relevant for any application relying on cDNA synthesis as an initial step of the procedure, such as RT-PCR, and microarray analysis...
  7. pmc Gene expression profiles from formalin fixed paraffin embedded breast cancer tissue are largely comparable to fresh frozen matched tissue
    Lorenza Mittempergher
    Divisions of Experimental Therapy, Molecular Biology, Surgical Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS ONE 6:e17163. 2011
    ..To this end we profiled, in duplicate, 20 FFPE tissues and 20 matched FF tissues and evaluated the concordance of the DASL results from FFPE and matched FF material...
  8. ncbi Automated extraction of DNA and RNA from a single formalin-fixed paraffin-embedded tissue section for analysis of both single-nucleotide polymorphisms and mRNA expression
    Guido Hennig
    Siemens Healthcare Diagnostics Products, Molecular Research Germany, Cologne, Germany
    Clin Chem 56:1845-53. 2010
    ..We investigated a fully automated, xylene-free nucleic acid extraction method for the simultaneous analysis of RNA and DNA biomarkers related to breast cancer...
  9. ncbi Cross-validation study for epidermal growth factor receptor and KRAS mutation detection in 74 blinded non-small cell lung carcinoma samples: a total of 5550 exons sequenced by 15 molecular French laboratories (evaluation of the EGFR mutation status for th
    Michèle Beau-Faller
    Laboratoire de Biologie Moleculaire, Hopital de Hautepierre, Strasbourg, France
    J Thorac Oncol 6:1006-15. 2011
    ....
  10. pmc Histological assessment of PAXgene tissue fixation and stabilization reagents
    Marcel Kap
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC, Rotterdam, The Netherlands
    PLoS ONE 6:e27704. 2011
    ..In general results obtained with PAXgene-fixed tissue are comparable to those of formalin-fixed tissue. Compromises made in morphology can be called minor compared to the advantages in the molecular pathology possibilities...
  11. pmc High resolution melting analysis for rapid and sensitive EGFR and KRAS mutation detection in formalin fixed paraffin embedded biopsies
    Hongdo Do
    Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, Locked Bag 1, A Beckett St, Melbourne, Victoria 8006, Australia
    BMC Cancer 8:142. 2008
    ..However, up to now it has not been fully validated for clinical samples when formalin-fixed paraffin-embedded (FFPE) sections are the only material available for analysis as is often the case...
  12. pmc Application of a BRAF pyrosequencing assay for mutation detection and copy number analysis in malignant melanoma
    Cynthia Spittle
    Clinical Translational Medicine, Oncology, Wyeth Research, 500 Arcola Rd, Collegeville, PA 19426, USA
    J Mol Diagn 9:464-71. 2007
    ..Pyro-sequencing serves as an excellent platform for BRAF genotyping of tumors from patients entering clinical trial...
  13. pmc Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples
    Yaguang Xi
    Cancer Genomics Laboratory, Mitchell Cancer Institute, Mobile, Alabama 36688, USA
    RNA 13:1668-74. 2007
    ..There are no significant differences of representative miRNA expression among 40 colorectal cancer FFPE specimens. This study provides a foundation for miRNA investigation using FFPE samples in cancer and other types of diseases...
  14. pmc Validation and reproducibility of a microarray-based gene expression test for tumor identification in formalin-fixed, paraffin-embedded specimens
    Raji Pillai
    Pathwork Diagnostics, Inc, Redwood City, California 94063 4737, USA
    J Mol Diagn 13:48-56. 2011
    ..3% concordance between laboratories. The Tissue of Origin Test makes the benefits of microarray-based gene expression tests for tumor diagnosis available for use with the most common type of histology specimen (ie, FFPE)...
  15. ncbi High recovery FASP applied to the proteomic analysis of microdissected formalin fixed paraffin embedded cancer tissues retrieves known colon cancer markers
    Jacek R Wisniewski
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Proteome Res 10:3040-9. 2011
    ..These results show that FASP is suitable for the low level analysis of microdissected tissue and that it has the potential for exploration of clinical samples for biomarker and drug target discovery...
  16. ncbi Generation of high-quality protein extracts from formalin-fixed, paraffin-embedded tissues
    Maria Filippa Addis
    Porto Conte Ricerche Srl, Tramariglio, Alghero, Sassari, Italy
    Proteomics 9:3815-23. 2009
    ..An evaluation of the extent of modifications introduced on proteins by formalin fixation and crosslink reversal, and their impact on quality of MS results, is also reported...
  17. ncbi DNA extraction from archival formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval principle: heating under the influence of pH
    Shan Rong Shi
    Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Histochem Cytochem 50:1005-11. 2002
    ..These findings may lead to a more effective protocol for DNA extraction from archival paraffin-embedded tissues and may also provide enhanced understanding of changes that occur during formalin-induced modification of nucleic acids...
  18. pmc A multiplex PCR predictor for aCGH success of FFPE samples
    E H van Beers
    Division of Experimental Therapy, Netherlands Cancer Institute NKI AvL, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Br J Cancer 94:333-7. 2006
    ....
  19. ncbi RNA expression analysis from formalin fixed paraffin embedded tissues
    Susan M Farragher
    Almac Diagnostics, Craigavon, 19 Seagoe Industrial Estate, Armagh BT635QD, UK
    Histochem Cell Biol 130:435-45. 2008
    Formalin fixation and paraffin embedding (FFPE) is the most commonly used method worldwide for tissue storage. This method preserves the tissue integrity but causes extensive damage to nucleic acids stored within the tissue...
  20. ncbi An improved and cost-effective methodology for the reduction of autofluorescence in direct immunofluorescence studies on formalin-fixed paraffin-embedded tissues
    M S Viegas
    Molecular Pathology Laboratory, Portuguese Institute for Oncology of Coimbra IPOCFG EPE, Coimbra, Portugal
    Eur J Histochem 51:59-66. 2007
    ..In addition, we herein show that this methodology is highly effective in reducing autofluorescent background to levels that allow detection of specific signals by direct immunofluorescence...
  21. ncbi Comparison of gene expression profiling by reverse transcription quantitative PCR between fresh frozen and formalin-fixed, paraffin-embedded breast cancer tissues
    Iker Sánchez-Navarro
    Laboratory of Molecular Pathology and Oncology, Research Unit, Hospital Universitario La Paz, Madrid, Spain
    Biotechniques 48:389-97. 2010
    ..Our results could serve as a guide when developing clinical diagnostic tests based on RT-qPCR analyses of FFPE tissues in the coming era of treatment decision-making based on gene expression profiling...
  22. ncbi Gene expression profiling of formalin-fixed, paraffin-embedded familial breast tumours using the whole genome-DASL assay
    Nic Waddell
    The Queensland Institute of Medical Research, Brisbane, Australia
    J Pathol 221:452-61. 2010
    ....
  23. pmc Quantitative expression profiling in formalin-fixed paraffin-embedded samples by affymetrix microarrays
    Diana Abdueva
    Department of Pathology, Children s Hospital Los Angeles, Research Institute and Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    J Mol Diagn 12:409-17. 2010
    ..This study provides guidelines for application of these methods in the discovery, validation, and clinical application of microarray expression profiling with FFPE material...
  24. pmc KRAS mutation: comparison of testing methods and tissue sampling techniques in colon cancer
    Wilbur A Franklin
    Department of Pathology, University of Colorado Denver, Fitzsimons Campus, 12801 E 17 Ave, Aurora, CO 80045, USA
    J Mol Diagn 12:43-50. 2010
    ..We conclude that coring of the paraffin blocks and testing by ARMS/S is a sensitive, specific, and efficient method for KRAS testing...
  25. pmc Equivalence of protein inventories obtained from formalin-fixed paraffin-embedded and frozen tissue in multidimensional liquid chromatography-tandem mass spectrometry shotgun proteomic analysis
    Robert W Sprung
    Jim Ayers Institute for Precancer Detection and Diagnosis, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 8575, USA
    Mol Cell Proteomics 8:1988-98. 2009
    ..These data demonstrate the equivalence of proteome inventories obtained from FFPE and frozen tissue specimens and provide support for retrospective proteomic analysis of FFPE tissues for biomarker discovery...
  26. ncbi Direct cancer tissue proteomics: a method to identify candidate cancer biomarkers from formalin-fixed paraffin-embedded archival tissues
    S I Hwang
    Department of Cell Biology, Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT 06030, USA
    Oncogene 26:65-76. 2007
    ..We propose that this general strategy provides a roadmap for successful identification of critical molecular targets of multiple cancer types...
  27. ncbi The use of formalin fixed wax embedded tissue for proteomic analysis
    Lynda D Ralton
    Department of Pathology, University of Aberdeen, Aberdeen, UK
    J Clin Pathol 64:297-302. 2011
    ..The purpose of this review is to provide an overview of the issues associated with the use of formalin fixed wax embedded tissues for proteomics...
  28. ncbi Tissue heterogeneity of EGFR mutation in lung adenocarcinoma
    Akira Sakurada
    Princess Margaret Hospital and Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada
    J Thorac Oncol 3:527-9. 2008
    ..This suggests that some tumors may demonstrate intratumoral heterogeneity for the occurrence of EGFR mutation...
  29. pmc A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections
    Hiroaki Nitta
    Medical Innovation, Ventana Medical Systems, Inc, Tucson, AZ, USA
    Diagn Pathol 7:60. 2012
    ....
  30. ncbi Efficient DNA extraction from 25-year-old paraffin-embedded tissues: study of 365 samples
    Anna Gillio-Tos
    Unit of Cancer Epidemiology, CeRMS, University of Turin, Italy
    Pathology 39:345-8. 2007
    ....
  31. pmc Extreme loss of immunoreactive p-Akt and p-Erk1/2 during routine fixation of primary breast cancer
    Isabel F Pinhel
    Department of Academic Biochemistry, Institute of Cancer Research, London, UK
    Breast Cancer Res 12:R76. 2010
    ..The aim of this study was to characterize the differences in immunoreactivity of common biomarkers that may occur (a) due to tissue handling at surgery and (b) between core-cuts and resected tumours...
  32. ncbi MicroRNA miR-17-5p is overexpressed in pancreatic cancer, associated with a poor prognosis, and involved in cancer cell proliferation and invasion
    Jun Yu
    Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Cancer Biol Ther 10:748-57. 2010
    ..0001 for both). The present findings suggest that miR-17-5p plays important roles in pancreatic carcinogenesis and cancer progression, and is associated with a poor prognosis in pancreatic cancer...
  33. pmc Report on emerging technologies for translational bioinformatics: a symposium on gene expression profiling for archival tissues
    Levi Waldron
    Department of Biostatistics and Computational Biology, Dana Farber Cancer Institute, Boston, MA, USA
    BMC Cancer 12:124. 2012
    ....
  34. ncbi Detection and quantitation of HER-2 gene amplification and protein expression in breast carcinoma
    Anna M Bofin
    Department of Laboratory Medicine, Children s and Women s Health, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim
    Am J Clin Pathol 122:110-9. 2004
    ..FISH may be used primarily or to confirm immunohistochemical, immunocytochemical, and PCR results...
  35. pmc One-step preservation of phosphoproteins and tissue morphology at room temperature for diagnostic and research specimens
    Claudius Mueller
    Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia, United States of America
    PLoS ONE 6:e23780. 2011
    ....
  36. ncbi MicroRNA expression in formalin-fixed paraffin embedded tissue using real time quantitative PCR: the strengths and pitfalls
    J R Dijkstra
    Department of Pathology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    J Cell Mol Med 16:683-90. 2012
    ..The purpose of this review is to describe some of these technical parameters and improve the validity and reliability of miRNA expression studies...
  37. ncbi Fluorescent in situ hybridization on tissue microarrays: challenges and solutions
    Lindsay A Brown
    Genetic Pathology Evaluation Centre of the Prostate Centre, University of British Columbia, Vancouver, BC, Canada
    J Mol Histol 38:151-7. 2007
    ..The problems encountered with FISH on TMAs, including probe preparation, hybridization, and potential applications of FISH, will be addressed in this review...
  38. pmc Validity of frozen sections for analysis of periprosthetic loosening membranes
    Stephan W Tohtz
    Center for Musculoskeletal Surgery, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Clin Orthop Relat Res 468:762-8. 2010
    ..6%, specificity 100%, and accuracy 96.2%. Our data support a recommendation for use of intraoperative frozen sections for diagnosis of septic versus aseptic loosening in revision hip surgery...
  39. ncbi Molecular platforms utilized to detect BRAF V600E mutation in melanoma
    Jonathan L Curry
    Department of Pathology, Section of Dermatopathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Semin Cutan Med Surg 31:267-73. 2012
    ....
  40. pmc Targeted high throughput sequencing in clinical cancer settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity
    Martin Kerick
    Max Planck Institute for Molecular Genetics, Ihnestr, Berlin, Germany
    BMC Med Genomics 4:68. 2011
    ..However, these technologies need to be further improved with regard to reproducibility and applicability to clinical samples and settings...
  41. ncbi Producing reverse phase protein microarrays from formalin-fixed tissues
    Claudia Wolff
    Institut fur Pathologie, Technische Universitat Munchen, Munich, Germany
    Methods Mol Biol 785:123-40. 2011
    ..In this chapter, we describe a protein extraction protocol established in our laboratory combined with the use of reverse phase protein microarray...
  42. ncbi Proteome, phosphoproteome, and N-glycoproteome are quantitatively preserved in formalin-fixed paraffin-embedded tissue and analyzable by high-resolution mass spectrometry
    Paweł Ostasiewicz
    Department for Proteomics and Signal Transduction at Max Planck Institute for Biochemistry, 82152 Martinsried, Germany
    J Proteome Res 9:3688-700. 2010
    ..Thus, FFPE biobank material can be analyzed by quantitative proteomics at the level of proteins and post-translational modifications...
  43. pmc Primary central nervous system lymphomas: a validation study of array-based comparative genomic hybridization in formalin-fixed paraffin-embedded tumor specimens
    Esteban Braggio
    Department of Biochemistry, Mayo Clinic, Scottsdale, Arizona, USA
    Clin Cancer Res 17:4245-53. 2011
    ....
  44. pmc Random DNA fragmentation allows detection of single-copy, single-exon alterations of copy number by oligonucleotide array CGH in clinical FFPE samples
    Galen Hostetter
    Translational Genomics Research Institute, 445 N 5th Street, Phoenix, AZ 85004, USA
    Nucleic Acids Res 38:e9. 2010
    ..3. Finally we present data on derivative of log ratio, a particular sensitive detector of measurement variance, for 216 sequential hybridizations to assess protocol reliability over a wide range of FFPE samples...
  45. pmc An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips
    Abdelhadi M Shebl
    Pathology Department, Faculty of Medicine, Mansoura University, Egypt
    Diagn Pathol 6:117. 2011
    ..This study was carried out in order to produce tissue microarray blocks using mechanical pencil tips without high cost...
  46. pmc Identification and validation of gene expression models that predict clinical outcome in patients with early-stage laryngeal cancer
    E Fountzilas
    Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Ann Oncol 23:2146-53. 2012
    ..Gene expression prognostic models could suggest which of these patients would be more appropriate for testing adjuvant strategies...
  47. ncbi Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue
    Stephen M Hewitt
    Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4605, USA
    Arch Pathol Lab Med 132:1929-35. 2008
    ..High-quality nucleic acids can be obtained from formalin-fixed, paraffin-embedded tissue, but greater attention to all steps in the process of tissue handling and preparation is required...
  48. ncbi Quick-multiplex-consensus (QMC)-PCR followed by high-resolution melting: a simple and robust method for mutation detection in formalin-fixed paraffin-embedded tissue
    Wakkas Fadhil
    Division of Pathology, School of Molecular Medical Sciences, University of Nottingham, Nottingham, UK
    J Clin Pathol 63:134-40. 2010
    ..Mutation detection in tumours will become increasingly important in pathological diagnosis as 'predictive' mutations are identified. A cheap and reliable test that works on formalin-fixed paraffin-embedded (FFPE) tissue is required...
  49. ncbi DNA extraction from archival formalin-fixed, paraffin-embedded tissues: heat-induced retrieval in alkaline solution
    Shan Rong Shi
    Department of Pathology, University of Southern California Keck School of Medicine HMR 204, 2011 Zonal Avenue, Los Angeles 90033, USA
    Histochem Cell Biol 122:211-8. 2004
    ..1 M alkaline solution is a simpler and more effective heat-induced retrieval protocol for DNA extraction. Combination with some chemicals (detergents) may further significantly improve efficiency of the heat-induced retrieval technique...
  50. pmc Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells
    Jinghuan Li
    Department of Histopathology, University of Dublin, Trinity College, Dublin, Ireland
    BMC Biotechnol 7:36. 2007
    ..This study analyzed 160 miRNAs in paired snap frozen and FFPE cells to investigate if miRNAs may be successfully detected in archival specimens...
  51. ncbi MicroRNA profiling of clear cell renal cell carcinoma by whole-genome small RNA deep sequencing of paired frozen and formalin-fixed, paraffin-embedded tissue specimens
    Lihong Weng
    Department of Pathology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    J Pathol 222:41-51. 2010
    ..To our knowledge, this is the first study to demonstrate that FFPE specimens can be used reliably for miRNA deep-sequencing analysis, making future large-scale clinical cohort/trial-based studies possible...
  52. pmc Multicentre validation study of nucleic acids extraction from FFPE tissues
    Serena Bonin
    ACADEM Department University of Trieste and ICGEB, University of Trieste, Cattinara Hospital, 34149 Trieste, Italy
    Virchows Arch 457:309-17. 2010
    ..The results of the study emphasize the need for quality control of the nucleic acid extracts with standardised methods to prevent false negative results and to allow data comparison among different diagnostic laboratories...
  53. pmc An array-based analysis of microRNA expression comparing matched frozen and formalin-fixed paraffin-embedded human tissue samples
    Xiao Zhang
    Department of Pathology and Molecular Medicine, Queen s Laboratory for Molecular Pathology, Kingston, ON, Canada
    J Mol Diagn 10:513-9. 2008
    ..669 to 0.815; Spearman rank correlation coefficient, 0.847 to 0.948), supporting the use of FFPE-derived miRNAs for array-based, gene expression profiling...
  54. ncbi Development and independent validation of a prognostic assay for stage II colon cancer using formalin-fixed paraffin-embedded tissue
    Richard D Kennedy
    Centre for Cancer Research and Cell Biology, Queen s University Belfast, Northern Ireland, United Kingdom
    J Clin Oncol 29:4620-6. 2011
    ..Here we describe a DNA microarray-based prognostic assay using clinically relevant formalin-fixed paraffin-embedded (FFPE) samples...
  55. pmc Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry
    Rita Casadonte
    Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA
    Nat Protoc 6:1695-709. 2011
    ..For example, TMA analysis of 300 FFPE cores would typically require 6 h of total time through data acquisition, not including data analysis...
  56. ncbi Analytical performance of a real-time PCR-based assay for V600 mutations in the BRAF gene, used as the companion diagnostic test for the novel BRAF inhibitor vemurafenib in metastatic melanoma
    Harkanwal Halait
    Roche Molecular Diagnostics, Pleasanton, CA 94588, USA
    Diagn Mol Pathol 21:1-8. 2012
    ..A simple 1:2 dilution resulted in a valid test result of 76% in such cases. The cobas test is a reproducible assay that detects some non-V600E mutations and is more accurate than direct sequencing in detecting BRAFV600E...
  57. ncbi Protein unlocking procedures of formalin-fixed paraffin-embedded tissues: application to MALDI-TOF imaging MS investigations
    Maurizio Ronci
    Centro Studi Sull Invecchiamento, Chieti, Italy
    Proteomics 8:3702-14. 2008
    ..A multivariate statistical evaluation is presented and discussed according to molecular spatial distributions and tissue morphology...
  58. ncbi 'Tissue surrogates' as a model for archival formalin-fixed paraffin-embedded tissues
    Carol B Fowler
    Department of Biophysics, Armed Forces Institute of Pathology, Rockville, MD 20850, USA
    Lab Invest 87:836-46. 2007
    ....
  59. ncbi Methods comparison for high-resolution transcriptional analysis of archival material on Affymetrix Plus 2.0 and Exon 1.0 microarrays
    Kim Linton
    Cancer Research UK Department of Medical Oncology, the Christie NHS Foundation Trust, Manchester, UK
    Biotechniques 47:587-96. 2009
    ..While larger series are needed to confirm high correspondence between fresh-frozen and FFPE expression patterns, these data suggest that both Plus 2.0 and Exon arrays are suitable platforms for FFPE microarray expression analyses...
  60. pmc Analyses and interpretation of whole-genome gene expression from formalin-fixed paraffin-embedded tissue: an illustration with breast cancer tissues
    Muhammad G Kibriya
    Department of Health Studies, The University of Chicago, 5841 S, Maryland Avenue, MC 2007, Chicago, IL 60637, USA
    BMC Genomics 11:622. 2010
    ..We used a whole genome DASL assay (addressing 18,391 genes) on a total of 72 samples from paired breast tumor and surrounding healthy tissues from both FF and FFPE samples...
  61. pmc KRAS genotyping of paraffin-embedded colorectal cancer tissue in routine diagnostics: comparison of methods and impact of histology
    Wilko Weichert
    Institut fur Pathologie, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    J Mol Diagn 12:35-42. 2010
    ....
  62. ncbi Formalin-fixed paraffin-embedded (FFPE) proteome analysis using gel-free and gel-based proteomics
    Omid Azimzadeh
    Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Institute of Radiation Biology, 85764 Neuherberg, Germany
    J Proteome Res 9:4710-20. 2010
    ..This study will facilitate the development of future proteomic analysis of FFPE tissue and provide a tool for the validation in archival samples of biomarkers of exposure, prognosis and disease...
  63. pmc A novel method of amplification of FFPET-derived RNA enables accurate disease classification with microarrays
    P Mickey Williams
    Roche Molecular Diagnostics, Pleasanton, California, USA
    J Mol Diagn 12:680-6. 2010
    ..An established classification model built from previous data accurately classified these new samples. This new method provides a useful technology advance for microarray analysis of FFPET archival samples...
  64. pmc Evaluation of non-formalin tissue fixation for molecular profiling studies
    John W Gillespie
    Pathogenetics Unit, Laboratory of Pathology, National Cancer Institute, Rm 2C500 Bldg 10, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Am J Pathol 160:449-57. 2002
    Using a general strategy for evaluating clinical tissue specimens, we found that 70% ethanol fixation and paraffin embedding is a useful method for molecular profiling studies. Human prostate and kidney were used as test tissues...
  65. pmc Enhanced stability of microRNA expression facilitates classification of FFPE tumour samples exhibiting near total mRNA degradation
    J S Hall
    Translational Radiobiology Group, School of Cancer and enabling Sciences, Manchester Academic Health Science Centre, The University of Manchester, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK
    Br J Cancer 107:684-94. 2012
    ....
  66. ncbi RNA extraction from archival formalin-fixed paraffin-embedded tissue: a comparison of manual, semiautomated, and fully automated purification methods
    Kerstin Bohmann
    Siemens Healthcare Diagnostics Products, Molecular Research Germany, Cologne, Germany
    Clin Chem 55:1719-27. 2009
    ..A robust and automated RNA-extraction method with a high sample throughput is required...
  67. pmc Clinical relevance of DNA microarray analyses using archival formalin-fixed paraffin-embedded breast cancer specimens
    Al Muktafi Sadi
    Ontario Cancer Institute and Campbell Family Institute for Breast Cancer Research, Princess Margaret Hospital, University Health Network, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada
    BMC Cancer 11:253:1-13. 2011
    ..A number of recent technical advances have been reported to overcome these issues. Our current study evaluates whether or not the technology is ready for clinical applications...
  68. ncbi Optimization of recovery of RNA from formalin-fixed, paraffin-embedded tissue
    Joon Yong Chung
    Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4605, USA
    Diagn Mol Pathol 15:229-36. 2006
    ..Pretreatment with RNA, later followed by formalin fixation, did not result in improving the RNA quality, but did improve RNA yield. Our method improves the utility of FFPE tissue for molecular profiling studies...
  69. ncbi Profiling signalling pathways in formalin-fixed and paraffin-embedded breast cancer tissues reveals cross-talk between EGFR, HER2, HER3 and uPAR
    Daniela Berg
    Institute of Pathology, Technische Universitat Muenchen, Munich, Germany
    J Cell Physiol 227:204-12. 2012
    ..Thus, targeting HER2, EGFR, HER3 and uPAR together may offer a more efficient treatment option for patients with breast cancer...
  70. pmc Fluorescence-based codetection with protein markers reveals distinct cellular compartments for altered MicroRNA expression in solid tumors
    Lorenzo F Sempere
    Department of Medicine, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA
    Clin Cancer Res 16:4246-55. 2010
    ..This ISH method was implemented to be compatible with routine clinical immunohistochemical (IHC) assays to enable the detection of miRNAs and protein markers in the same tissue section for colocalization and functional studies...
  71. ncbi Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues
    John B A Okello
    McMaster Ancient DNA Centre, Department of Anthropology, McMaster University, Hamilton, Ontario L8S4L9, Canada
    Anal Biochem 400:110-7. 2010
    ....
  72. ncbi Frequency of KRAS, BRAF, and PIK3CA mutations in advanced colorectal cancers: Comparison of peptide nucleic acid-mediated PCR clamping and direct sequencing in formalin-fixed, paraffin-embedded tissue
    Mi Jung Kwon
    Department of Pathology, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang, Republic of Korea
    Pathol Res Pract 207:762-8. 2011
    ..The PNA-mediated PCR clamping method is highly sensitive and is efficiently applicable to the detection of KRAS, BRAF, and PIK3CA mutations in a clinical setting...
  73. pmc Analytical performance of a PCR assay for the detection of KRAS mutations (codons 12/13 and 61) in formalin-fixed paraffin-embedded tissue samples of colorectal carcinoma
    Sung Lee
    Roche Molecular Systems, Inc, 4300 Hacienda Drive, Pleasanton, CA 94588, USA
    Virchows Arch 460:141-9. 2012
    ..The PCR assay was more sensitive and specific than Sanger sequencing, and performance was highly reproducible. Test performance was not influenced by various endogenous interfering substances or common gut microbes...
  74. ncbi A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues
    Teresa Rodríguez-Rigueiro
    Translational Cancer Research Group, Proteo Red ISCIII, Instituto de Investigación Biomédica A Coruña, Coruna, Spain
    Proteomics 11:2555-9. 2011
    ..This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases...
  75. pmc miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR
    Dario de Biase
    Department of Patologia Sperimentale, University of Bologna, Bologna, Italy
    PLoS ONE 7:e35596. 2012
    ..To the best of our knowledge this is the first paper demonstrating that the results obtained in miRNAs analysis using Real-Time PCR starting from FFPE specimens of glioblastoma are comparable with those obtained in Fresh/Frozen samples...
  76. pmc A rapid and reliable test for BRCA1 and BRCA2 founder mutation analysis in paraffin tissue using pyrosequencing
    Liying Zhang
    Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    J Mol Diagn 11:176-81. 2009
    ..We show that pyrosequencing is a rapid and reliable method that serves as an excellent platform for BRCA founder mutation analysis, especially when only paraffin-embedded tissues are available...
  77. pmc Tissues from routine pathology archives are suitable for microRNA analyses by quantitative PCR
    U Siebolts
    Institute of Pathology, University Hospital of Cologne, Cologne, Germany
    J Clin Pathol 62:84-8. 2009
    ..MicroRNAs have recently taken centre stage as short non-coding RNAs that regulate mRNA expression...
  78. ncbi Citric acid antigen retrieval (CAAR) for tryptic peptide imaging directly on archived formalin-fixed paraffin-embedded tissue
    Johan O R Gustafsson
    Adelaide Proteomics Centre, School of Molecular and Biomedical Science, The University of Adelaide, SA 5005, Adelaide, Australia
    J Proteome Res 9:4315-28. 2010
    ..The CAAR method presented here complements previously described antigen-retrieval protocols and is an important step in being able to fully analyze the proteome of archived FFPE tissue...
  79. pmc Elevated pressure improves the extraction and identification of proteins recovered from formalin-fixed, paraffin-embedded tissue surrogates
    Carol B Fowler
    Department of Biophysics, Armed Forces Institute of Pathology, Rockville, Maryland, United States of America
    PLoS ONE 5:e14253. 2010
    ..We previously reported the use of elevated hydrostatic pressure as a method for efficient protein recovery from a hen egg-white lysozyme tissue surrogate, a model system developed to study formalin fixation and histochemical processing...
  80. ncbi MALDI direct analysis and imaging of frozen versus FFPE tissues: what strategy for which sample?
    Maxence Wisztorski
    Laboratoire de Neuroimmunologie et Neurochimie Evolutives, FRE CNRS 3249, MALDI Imaging Team, Universite Lille Nord de France, Universite Lille 1, Villeneuve d Ascq Cedex, France
    Methods Mol Biol 656:303-22. 2010
    ..We present methods and protocols for FFPE samples. These strategies have proven to be valuable for all tested FFPE samples and have opened archived tissues from hospital banks to MALDI-MSI...
  81. ncbi RNA expression analysis on formalin-fixed paraffin-embedded tissues in TMA format by RNA in situ hybridization
    Jürgen Veeck
    Molecular Oncology Group, Institute of Pathology, University Hospital of the RWTH Aachen, Aachen, Germany
    Methods Mol Biol 664:135-50. 2010
    ..A protocol is presented here that has been routinely and successfully used on FFPE tissues assembled on a tissue micro array (TMA)...
  82. ncbi Analysis of DNA methylation in FFPE tissues using the MethyLight technology
    Ashraf Dallol
    King Fahad Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
    Methods Mol Biol 724:191-204. 2011
    ..We describe in this chapter a detailed protocol for MethyLight and its use in the determination of CpG Island Methylator Phenotype status in FFPE colorectal cancer samples...
  83. ncbi Gene expression analysis using long-term preserved formalin-fixed and paraffin-embedded tissue of non-small cell lung cancer
    Therese A S Jacobson
    Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden
    Int J Oncol 38:1075-81. 2011
    ..However, a general interpretation must be done with caution as different FFPE procedures have varying effects on RNA quality...
  84. pmc Molecular characterisation of formalin-fixed paraffin-embedded (FFPE) breast tumour specimens using a custom 512-gene breast cancer bead array-based platform
    M Abramovitz
    VM Institute of Research, 2020 University Street, Montreal, Quebec H3A 2A5, Canada
    Br J Cancer 105:1574-81. 2011
    ..In order to extend the utility of the DASL assay for breast cancer, we have custom designed and validated a 512-gene human breast cancer panel...
  85. pmc Comparison of histopathology to gene expression profiling for the diagnosis of metastatic cancer
    Anand Kulkarni
    University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Diagn Pathol 7:110. 2012
    ..Gene expression profiling (GEP) has found increasing use, particularly in the most difficult cases. In this pilot study, a direct comparison between GEP and IHC-guided methods was performed...
  86. pmc FGFR2 gene amplification and clinicopathological features in gastric cancer
    K Matsumoto
    Department of Genome Biology, Kinki University Faculty of Medicine, Osaka 589 8511, Japan
    Br J Cancer 106:727-32. 2012
    ..Frequency of FGFR2 amplification, its clinicopathological features, and the results of high-throughput screening assays in a large cohort of gastric clinical samples remain largely unclear...
  87. pmc Limited copy number-high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies
    Hongdo Do
    Department of Pathology, University of Melbourne, Parkville, Victoria, 3010, Australia
    Mol Cancer 8:82. 2009
    ..The dilutions used (based on Ct values) are chosen such that mutations, if present, can be detected by the direct sequencing of amplicons with aberrant melting patterns...
  88. pmc mRNA and micro-RNA expression analysis in laser-capture microdissected prostate biopsies: valuable tool for risk assessment and prevention trials
    Larisa Nonn
    Department of Pathology, University of Illinois at Chicago, Chicago, IL 60612, USA
    Exp Mol Pathol 88:45-51. 2010
    ..With careful technique and use of appropriate controls, RNA profiling from archived biopsy material is quite feasible showing high correlation to frozen tissue...
  89. pmc Multiple mutation analyses in single tumor cells with improved whole genome amplification
    W Dietmaier
    Institute of Pathology, The Department of Surgery, University of Regensburg, Germany
    Am J Pathol 154:83-95. 1999
    ....
  90. pmc Using oligonucleotide aptamer probes for immunostaining of formalin-fixed and paraffin-embedded tissues
    Zihua Zeng
    Department of Pathology, The Methodist Hospital and The Methodist Hospital Research Institute, Houston, TX 77030, USA
    Mod Pathol 23:1553-8. 2010
    ..Therefore, our findings indicate that the synthetic oligonucleotide CD30 aptamer can be used as a probe for immunostaining of fixed tissue sections for disease diagnosis...
  91. pmc Evaluation of archival time on shotgun proteomics of formalin-fixed and paraffin-embedded tissues
    Brian M Balgley
    Calibrant Biosystems, Gaithersburg, Maryland 20878, USA
    J Proteome Res 8:917-25. 2009
    ....
  92. ncbi Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting
    Niroshini J Nirmalan
    Clinical and Biomedical Proteomics Group, Cancer Research UK Clinical Centre, Leeds, UK
    J Pathol 217:497-506. 2009
    ....
  93. pmc Protein extraction of formalin-fixed, paraffin-embedded tissue enables robust proteomic profiles by mass spectrometry
    Marshall S Scicchitano
    Department of Safety Assessment, 709 Swedeland Road, Mail Stop UE0364, King of Prussia, PA 19406, USA
    J Histochem Cytochem 57:849-60. 2009
    ..These findings demonstrate that formalin fixation, paraffin processing, and LCM do not negatively impact protein quality and quantity as determined by MS and that FFPE samples are amenable to global proteomic analysis...
  94. ncbi Effect of duration of fixation on quantitative reverse transcription polymerase chain reaction analyses
    Maricris Macabeo-Ong
    Oral Pathology, Department of Stomatology, University of California San Francisco, California 94143 0424, USA
    Mod Pathol 15:979-87. 2002
    ..However, results for some markers are adversely affected by prolonged formalin fixation times...
  95. ncbi Clinical proteomic applications of formalin-fixed paraffin-embedded tissues
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, National Cancer Institute at Frederick, 1050 Boyles Street, Frederick, MD 21702, USA
    Clin Lab Med 29:101-13. 2009
    ..The almost inexhaustible supply of these tissues will enable proteomic laboratories access to clinically important specimens that will undoubtedly lead to a number of important discoveries in the near future...
  96. ncbi Quantitation of p95HER2 in paraffin sections by using a p95-specific antibody and correlation with outcome in a cohort of trastuzumab-treated breast cancer patients
    Jeff Sperinde
    diaDexus, South San Francisco, CA, USA
    Clin Cancer Res 16:4226-35. 2010
    ..We sought to show that quantitative p95HER2 levels would correlate with outcome in trastuzumab-treated HER2-positive metastatic breast cancer...
  97. ncbi Chromosomal instability is a risk factor for poor prognosis of adenocarcinoma of the lung: Fluorescence in situ hybridization analysis of paraffin-embedded tissue from Korean patients
    Chang Min Choi
    Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, South Korea
    Lung Cancer 64:66-70. 2009
    ..The relationship between CIN detected by fluorescence in situ hybridization (FISH) and survival in AC patients was examined...
  98. ncbi Genome-wide DNA methylation analysis of archival formalin-fixed paraffin-embedded tissue using the Illumina Infinium HumanMethylation27 BeadChip
    Christina Thirlwell
    Medical Genomics Group, UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, UK
    Methods 52:248-54. 2010
    ..Here, we describe a protocol for the analysis of FFPE samples using the Infinium HumanMethylation27 BeadChip...
  99. ncbi Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM)
    W Baschong
    M E Muller Institute at the Biozentrum, Aarau, Switzerland
    J Histochem Cytochem 49:1565-72. 2001
    ..Sudan Black B is suggested to mask fluorescent tissue components...
  100. pmc Genome-wide massively parallel sequencing of formaldehyde fixed-paraffin embedded (FFPE) tumor tissues for copy-number- and mutation-analysis
    Michal R Schweiger
    Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
    PLoS ONE 4:e5548. 2009
    ..However, FFPE preparation is incompatible with many down-stream molecular biology techniques such as PCR based amplification methods and gene expression studies...
  101. pmc Exon-array profiling unlocks clinically and biologically relevant gene signatures from formalin-fixed paraffin-embedded tumour samples
    J S Hall
    Translational Radiobiology Group, School of Cancer and enabling Sciences, Manchester Academic Health Science Centre, The University of Manchester, Wilmslow Road, Manchester M20 4BX, UK
    Br J Cancer 104:971-81. 2011
    ..This study aimed to show that useful information can be obtained by Exon-array profiling archival FFPE tumour samples...

Research Grants44

  1. Implementation of phosphoprotein preservation technology for cancer biospecimens
    LANCE ALLEN LIOTTA; Fiscal Year: 2013
    ..Phosphoprotein antigen epitopes are not adequately preserved by formalin fixation and paraffin embedding, and freezing of tissue is very expensive and compromises diagnostic accuracy...
  2. Tissue procurement &processing for molecular analysis
    Christopher A Moskaluk; Fiscal Year: 2013
    ..In addition to paraffin embedding, histologic sections of paraffin embedded or frozen tissue are offered...
  3. Lung cancer heterogeneity and its impact on drug resistance.
    Steven J Altschuler; Fiscal Year: 2013
    ..The final aim tests the feasibility of translating image-based assays to frozen and formalin fixed, paraffin embedding (FFPE) primary patient samples...
  4. Confocal enhanced episcopic fluorescent image capture (EFIC)
    Cecilia W Lo; Fiscal Year: 2012
    ..we will assemble a 2nd generation EFIC imaging system that will obviate the need for high temperature paraffin embedding with aniline. Imaging will be carried out with the Leica widefield superzoom LSI confocal macroscope...
  5. Develop & Validate An Ultrasound Tissue Preservation De*
    Zhenqing Zhu; Fiscal Year: 2006
    ..of a commercialized intensity adjustable bench top fixer/processor for rapid formalin fixation and paraffin embedding, 2) Evaluate and validate the DTP method in comparison to conventional FFPE method based on preservation of ..
  6. microRNAs as novel Biomarkers for Pancreatic Ductal Adenocarcinoma
    Murray Korc; Fiscal Year: 2009
    ....
  7. Ultrasound-accelerated tissue formalin fixation and paraffin embedding
    Wei Sing Chu; Fiscal Year: 2008
    ..Formalin fixation and paraffin embedding (FFPE) has been a standard tissue preservation method employed in over 90% cases for clinical histology ..
  8. Fine Needle Aspiration Biopsy(FNAB) Needle Device
    Vinod Shidham; Fiscal Year: 2002
    ..Apart from the worldwide market, the potential market in United States alone should exceed $1,500,000 per year. ..
  9. Seattle Cancer and Aging Program
    Peter Rabinovitch; Fiscal Year: 2007
    ..We fully expect that the activities outlined in this proposal will lead to important discovery and lay the foundation for significant scientific effort in the future. ..
  10. Taste Quality Coding Of Salts
    STEVEN ST JOHN; Fiscal Year: 2004
    ....
  11. Training in the Neurobiology of Otorhinolaryngology
    James Saunders; Fiscal Year: 2007
    ..Research is conducted under the supervision of one of the trainers. We are requesting four predoctoral, three postdoctoral positions per year. [unreadable] [unreadable]..
  12. Prognostic Significance of DNA and Histone Methylation
    Chandrika Piyathilake; Fiscal Year: 2007
    ..Investigator talent, equipment, facilities and access to study subjects at UAB are ideal for conducting this study. ..
  13. (m) TOR signaling in EBV-associated lymphomas
    Mariusz Wasik; Fiscal Year: 2007
    ..role of TOR signaling in cell transformation of the lymphoma cells These studies may result in novel diagnostic and therapeutic approaches to the EBV-associated lymphomas in HIV/AIDS and other patients. ..
  14. Choroid plexus & antidepressants: genomics & proteomics
    SAMUEL SATHYANESAN; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  15. Preclin. model for prevention of NSCLC in former smokers
    HILDEGARD SCHULLER; Fiscal Year: 2006
    ....
  16. THE EFFECT OF LYCOPENE ON IGF-1 ACTIVITY AND SECRETION IN PRIMARY CULTURES OF PRO
    Larisa Nonn; Fiscal Year: 2008
    ..Once we have determined lycopene's effect on the IGF-1-induced actions in normal prostatic cells, this effect will be compared to those on cells derived from adenocarcinoma. [unreadable] [unreadable] [unreadable]..
  17. CaP, crystal responsible for idiopathic nephrolithasis
    Saeed Khan; Fiscal Year: 2008
    ..Type density and size must conform to limits and specifications provided in the PHS 398Instructions. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..
  18. University of the District of Columbia and the Lombardi*
    Peter Shields; Fiscal Year: 2008
    ..Each institution has expressed in writing a commitment to insure the success of the Partnership. ..
  19. HPV Clearance by Folic Acid Supplementation
    Chandrika J Piyathilake; Fiscal Year: 2010
    ..Because this study will be conducted among women positive for HPV 16, the most prevalent and powerful causative agent of CC, the public health significance of this study could be enormous and will complement HPV vaccine studies. ..
  20. Ehrlichia chaffeensis protective proteins
    David H Walker; Fiscal Year: 2010
    ..chaffeensis. ..
  21. Jak/STAT signaling in human T-cell lymphomas
    Mariusz A Wasik; Fiscal Year: 2010
    ....
  22. Disclosure of Results from Genetic Research Studies
    Lynn Dressler; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  23. Molecular Epidemiology of Secondary Lung Cancer
    Peter Shields; Fiscal Year: 2007
    ..RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page .................................................................................................................................................. 1 Description, ..
  24. The Emergence of HIV: A Molecular Archeology Approach
    Michael Worobey; Fiscal Year: 2006
    ..abstract_text> ..
  25. POLYVALENT VACCINE--PHASE III TRIAL IN STAGE IV MELANOMA
    Donald Morton; Fiscal Year: 2002
    ..Determine whether there is any correlation between such responses and the clinical course of vaccine patients in a prospective multicenter trial. ..
  26. Prognostic Significance of Altered DNA Methylation
    Chandrika Piyathilake; Fiscal Year: 2002
    ..A newly developed and tested immunohistochemical technique, which measures the degree of global methylation in intact and specific types of cells, will be used to evaluate global DNA methylation in the proposed study. ..
  27. Novel Bisphosphonates for Breast Cancer Therapy
    MONICA REINHOLZ; Fiscal Year: 2002
    ..Proposed Commercial Application: Not Available. ..
  28. Herpesviruses in Vestibular Neuritis
    JEFFREY VRABEC; Fiscal Year: 2003
    ..This information can help determine why some individuals with latent virus in the vestibular ganglion develop clinical symptoms due to reactivation and some do not. ..
  29. Flow Cytometry to Detect Human Tumor Cells in Body Fluid
    AWTAR GANJU KRISHAN; Fiscal Year: 2003
    ..3. Compare data collected from flow cytometric analysis with that obtained from cytopathology and immunocytochemistry routinely performed on these specimens for diagnostic purpose. ..
  30. Novel Bisphosphonates for Multiple Myeloma Therapy
    MONICA REINHOLZ; Fiscal Year: 2004
    ..Successful development of this new drug-design concept would provide a biologically and commercially superior approach for the treatment and prevention of skeletal-related complications of malignancy. ..
  31. MURINE MODEL FOR MONKEY B VIRUS INFECTION
    JERRY RITCHEY; Fiscal Year: 2004
    ..abstract_text> ..
  32. GENE-EXPRESSION CHANGE IN HUMAN BLOOD SAMPLES AND ANTI-DEPRESSANT RESPONSE
    SAMUEL SATHYANESAN; Fiscal Year: 2004
    ..Briefly, further studies would involve examening differences among different types of depressive disorders and the correlation between gene-expression changes and treatment-response. ..
  33. Effects of Folate Fortification on Cancer Prevention an*
    Chandrika Piyathilake; Fiscal Year: 2005
    ....
  34. NNK, Beta-Adrenergic AA Release and Lung Cancer
    HILDEGARD SCHULLER; Fiscal Year: 2005
    ..2. To determine which products of the AA cascade are formed in untreated cells and in response to NNK, which of these products stimulate DNA synthesis, and what downstream effectors are activated by these products. ..
  35. Simultaneous PET imaging of rCBF and dopamine release
    ALEXANDER CONVERSE; Fiscal Year: 2006
    ....
  36. Defining Cell Lineages in the Bladder Urothelium
    Sabina Signoretti; Fiscal Year: 2006
    ..These studies will clarify lineage relationships among bladder epithelial cells and provide important insights into the nature of urothelial stem cells. ..
  37. Tumor suppressor gene at 6q in adenoid cystic carcinoma
    Christopher Moskaluk; Fiscal Year: 2006
    ..Since AGO appears to have a smaller number of genetic changes than these other malignancies, it may serve as a simpler system to identify the 6q tumor suppressor gene. ..
  38. FLOW CYTOMETER--SHARED EQUIPMENT--CELL SORTER
    Peter Rabinovitch; Fiscal Year: 2001
    ..The present excess demand for access to flow cytometry and the need for additional capabilities, including high speed sorting, are now limitations to the progress of many NIH funded research projects at the University of Washington...
  39. Gammacell-220 Excel Refurbished Research Irradiator
    David Walker; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  40. HAIR CELL PATHOPHYSIOLOGY
    James Saunders; Fiscal Year: 2006
    ..Cochlear nerve abnormal rate-intensity functions two-weeks post exposure originate from tall hair cells adjacent to the "patch" lesion. ..
  41. TUMOR MARKERS TO PREDICT TREATMENT RESPONSE
    Lynn Dressler; Fiscal Year: 2005
    ..Two subcontracts are included in this proposal, one to support the CALGB Pathology Coordinating Office functions at OSU relevant to this study and the other in support of the molecular studies being performed by USC. ..
  42. AMYLOIDOGENIC EFFECTS OF CHOLINERGIC DENERVATION
    Thomas Beach; Fiscal Year: 2002
    ..This could lead to impaired cerebral vasoregulation and increased susceptibility to ischemia, further compromising cortical function in AD. ..
  43. Preparation of Cancer Tissues for MS Imaging of Proteins
    Pierre Chaurand; Fiscal Year: 2007
    ..We also propose to further develop and validate protocols for the molecular analysis by MS of cancer cells in fine needle aspirates. Finally, we propose to automate some key aspects of these methodologies. ..
  44. TISSUE-CRYSTAL INTERACTION CA OXALATE NEPHROLITHIASIS
    Saeed Khan; Fiscal Year: 2003
    ..We can follow various events of nephrolithiasis in the animal model and cats explore the outcome of renal epithelial cell exposure to oxalate and calcium oxalate crystals in the cell cultures. ..