staining and labeling


Summary: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.

Top Publications

  1. ncbi Quantification of histochemical staining by color deconvolution
    A C Ruifrok
    Department of Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA
    Anal Quant Cytol Histol 23:291-9. 2001
  2. ncbi HaloTag: a novel protein labeling technology for cell imaging and protein analysis
    Georgyi V Los
    Promega Corporation, 2800 Woods Hollow Rd, Madison, Wisconsin 53711, USA
    ACS Chem Biol 3:373-82. 2008
  3. ncbi A general method for the covalent labeling of fusion proteins with small molecules in vivo
    Antje Keppler
    Institute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne EPFL, CH 1015, Lausanne, Switzerland
    Nat Biotechnol 21:86-9. 2003
  4. ncbi Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast
    Lyris M F de Godoy
    Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nature 455:1251-4. 2008
  5. ncbi Quantum dot bioconjugates for imaging, labelling and sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Nat Mater 4:435-46. 2005
  6. pmc In vivo MRI cell tracking: clinical studies
    Jeff W M Bulte
    Russell H Morgan Department of Radiology, Division of MR Research, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    AJR Am J Roentgenol 193:314-25. 2009
  7. pmc Bioluminescent imaging of Trypanosoma brucei shows preferential testis dissemination which may hamper drug efficacy in sleeping sickness
    Filip Claes
    Institute of Tropical Medicine Antwerp, Department of Parasitology, Antwerp, Belgium
    PLoS Negl Trop Dis 3:e486. 2009
  8. pmc Labeling HIV-1 virions with two fluorescent proteins allows identification of virions that have productively entered the target cell
    Edward M Campbell
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Ward 8 140, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Virology 360:286-93. 2007
  9. ncbi Phosphorescent heavy-metal complexes for bioimaging
    Qiang Zhao
    Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, P R China
    Chem Soc Rev 40:2508-24. 2011
  10. ncbi Cell tagging with clinically approved iron oxides: feasibility and effect of lipofection, particle size, and surface coating on labeling efficiency
    Lars Matuszewski
    Department of Clinical Radiology, University Hospital Muenster, Albert Schweitzer Strasse 33, D 48129 Muenster, Germany
    Radiology 235:155-61. 2005

Research Grants

Detail Information

Publications338 found, 100 shown here

  1. ncbi Quantification of histochemical staining by color deconvolution
    A C Ruifrok
    Department of Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA
    Anal Quant Cytol Histol 23:291-9. 2001
  2. ncbi HaloTag: a novel protein labeling technology for cell imaging and protein analysis
    Georgyi V Los
    Promega Corporation, 2800 Woods Hollow Rd, Madison, Wisconsin 53711, USA
    ACS Chem Biol 3:373-82. 2008
  3. ncbi A general method for the covalent labeling of fusion proteins with small molecules in vivo
    Antje Keppler
    Institute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology Lausanne EPFL, CH 1015, Lausanne, Switzerland
    Nat Biotechnol 21:86-9. 2003
    ..Here we describe a general method for the covalent labeling of fusion proteins in vivo that complements existing methods for noncovalent labeling of proteins and that may open up new ways of studying proteins in living cells...
  4. ncbi Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast
    Lyris M F de Godoy
    Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nature 455:1251-4. 2008
    ..Systems-wide, precise quantification directly at the protein level opens up new perspectives in post-genomics and systems biology...
  5. ncbi Quantum dot bioconjugates for imaging, labelling and sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Nat Mater 4:435-46. 2005
    ..The potential of QDs in biology has just begun to be realized and new avenues will arise as our ability to manipulate these materials improves...
  6. pmc In vivo MRI cell tracking: clinical studies
    Jeff W M Bulte
    Russell H Morgan Department of Radiology, Division of MR Research, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    AJR Am J Roentgenol 193:314-25. 2009
    ..The purpose of this review is to describe the principles of MRI cell tracking with superparamagnetic iron oxides and the four clinical trials that have been performed...
  7. pmc Bioluminescent imaging of Trypanosoma brucei shows preferential testis dissemination which may hamper drug efficacy in sleeping sickness
    Filip Claes
    Institute of Tropical Medicine Antwerp, Department of Parasitology, Antwerp, Belgium
    PLoS Negl Trop Dis 3:e486. 2009
    ..brucei-infected mice. We showed that parasites located in the testis, as compared to those located in the abdominal cavity, were not readily cleared by the drugs...
  8. pmc Labeling HIV-1 virions with two fluorescent proteins allows identification of virions that have productively entered the target cell
    Edward M Campbell
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Ward 8 140, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Virology 360:286-93. 2007
    ..This increased ability to discriminate between non-productively endocytosed virions and those actively undergoing steps in the infectious process will facilitate efforts to examine early steps in infection microscopically...
  9. ncbi Phosphorescent heavy-metal complexes for bioimaging
    Qiang Zhao
    Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, P R China
    Chem Soc Rev 40:2508-24. 2011
    ..In addition, several possible future directions in this field are also discussed (133 references)...
  10. ncbi Cell tagging with clinically approved iron oxides: feasibility and effect of lipofection, particle size, and surface coating on labeling efficiency
    Lars Matuszewski
    Department of Clinical Radiology, University Hospital Muenster, Albert Schweitzer Strasse 33, D 48129 Muenster, Germany
    Radiology 235:155-61. 2005
    ..To evaluate the effect of lipofection, particle size, and surface coating on labeling efficiency of mammalian cells with superparamagnetic iron oxides (SPIOs)...
  11. ncbi Transfection of neuroprogenitor cells with iron nanoparticles for magnetic resonance imaging tracking: cell viability, differentiation, and intracellular localization
    Sosuke Miyoshi
    Department of Bioengineering, University of Washington, Seattle, USA
    Mol Imaging Biol 7:286-95. 2005
    ..The use of hemagglutinating virus of Japan envelopes (HVJ-Es) to effectively introduce the contrast agent to neural progenitor cells (NPCs) is limited to date despite their high NPC affinity...
  12. pmc Rapid method for enumeration of viable Legionella pneumophila and other Legionella spp. in water
    Pilar Delgado-Viscogliosi
    Département Eaux et Environnement, Institut Pasteur de Lille, F 59019 Lille cedex, France
    Appl Environ Microbiol 71:4086-96. 2005
  13. ncbi Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: a study using the mouse model
    Yan Guang Wu
    Laboratory for Animal Reproduction and Embryology, College of Animal Science and Vet Medicine, Shandong Agricultural University, Tai an, Shandong, China
    Cell Res 17:722-31. 2007
    ..Taken together, the series of criteria described here would allow for better choices in selecting oocytes for better development...
  14. ncbi Iron-oxide labeling and outcome of transplanted mesenchymal stem cells in the infarcted myocardium
    Yoram Amsalem
    Neufeld Cardiac Research Institute, Sheba Medical Center, Tel Hashomer 52621, Israel
    Circulation 116:I38-45. 2007
    ..We sought to determine whether mesenchymal stem cell (MSC) outcome is affected by SPIO labeling in a rat model of myocardial infarction...
  15. pmc Pharmacological rescue of the mutant cystic fibrosis transmembrane conductance regulator (CFTR) detected by use of a novel fluorescence platform
    John P Holleran
    Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    Mol Med 18:685-96. 2012
    ..These findings indicate that the FAP-tagged constructs faithfully report mutant CFTR correction activity and that this approach should be useful as a screening assay in diseases that impair protein trafficking to the cell surface...
  16. ncbi Differential effects of environmental chemicals and food contaminants on adipogenesis, biomarker release and PPARγ activation
    Camilla Taxvig
    Division of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark, Søborg, Denmark
    Mol Cell Endocrinol 361:106-15. 2012
    ..Eight chemicals were able to cause increased leptin release. These findings strengthen the hypothesis that chemicals can interfere with pathways related to obesity development...
  17. ncbi Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR
    M Adela Yáñez
    Labaqua, SA, Alicante, Spain
    J Microbiol Methods 85:124-30. 2011
    ..For future research it would be desirable to increase the method's capacity to exclude signals from dead cells in difficult matrices or samples containing high numbers of dead cells...
  18. ncbi Multifunctional protein labeling via enzymatic N-terminal tagging and elaboration by click chemistry
    William P Heal
    Department of Chemistry, Imperial College London, UK
    Nat Protoc 7:105-17. 2012
  19. pmc An adaptable two-color flow cytometric assay to quantitate the invasion of erythrocytes by Plasmodium falciparum parasites
    Michel Theron
    Sanger Malaria Programme, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Cytometry A 77:1067-74. 2010
    ..The assay is sensitive, operates in a 96-well format, and can be used to quantitate the impact of natural or experimental genetic variation on erythrocyte invasion efficiency...
  20. ncbi Mesenchymal stem cell labeling and in vitro MR characterization at 1.5 T of new SPIO contrast agent: Molday ION Rhodamine-B™
    Benjamin Addicott
    Diabetes Research Institute, University of Miami School of Medicine, FL 33101, USA
    Contrast Media Mol Imaging 6:7-18. 2011
    ..Properties evaluated in this study will be used as a reference for tracking labeled MSC for in vivo studies...
  21. ncbi Improvement of the green fluorescent protein reporter system in Leishmania spp. for the in vitro and in vivo screening of antileishmanial drugs
    Sergio A Pulido
    Program for Study and Control of Tropical Diseases, School of Medicine, University of Antioquia, Medellin, Colombia
    Acta Trop 122:36-45. 2012
  22. ncbi MSSimulator: Simulation of mass spectrometry data
    Chris Bielow
    Institute of Computer Science, Department of Mathematics and Computer Science, Freie Universität Berlin, Berlin, Germany
    J Proteome Res 10:2922-9. 2011
    ..Several protocols for SILAC, iTRAQ or MS(E) are available, in addition to the usual label-free approach, making MSSimulator the most comprehensive simulator for LC-MS and LC-MS/MS data...
  23. ncbi Double-labelled HIV-1 particles for study of virus-cell interaction
    Marko Lampe
    Department of Virology, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    Virology 360:92-104. 2007
    ..We present here a detailed characterization of this novel tool together with exemplary live cell imaging studies, demonstrating its suitability for real-time analyses of HIV-cell interaction...
  24. ncbi Finding fluorescent needles in the cardiac haystack: tracking human mesenchymal stem cells labeled with quantum dots for quantitative in vivo three-dimensional fluorescence analysis
    Amy B Rosen
    Institute for Molecular Cardiology, State University of New York at Stony Brook, Stony Brook, NY 11794, USA
    Stem Cells 25:2128-38. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  25. ncbi In vivo magnetic resonance imaging of injected endothelial progenitor cells after myocardial infarction in rats
    Yuyu Yao
    Department of Cardiology, Zhongda Hospital, Southeast University, 87 DingJiaQiao, Nanjing, Jiangsu, 210009, China
    Mol Imaging Biol 13:303-13. 2011
    ..The purpose of this study was to detect and follow transplanted superparamagnetic iron oxide (SPIO)-labeled endothelial progenitor cells (EPCs) by magnetic resonance imaging (MRI)...
  26. pmc Fluorescent labeling of tetracysteine-tagged proteins in intact cells
    Carsten Hoffmann
    Department of Pharmacology and Toxicology, University of Wurzburg, Germany
    Nat Protoc 5:1666-77. 2010
    ..The labeling procedure using FlAsH-EDT₂ as described takes 2-3 h, depending on the number of samples to be processed...
  27. pmc Detection and quantification of magnetically labeled cells by cellular MRI
    Wei Liu
    Philips Research North America, Briarcliff Manor, NY 10510, USA
    Eur J Radiol 70:258-64. 2009
    ..This review summarizes the practical issues regarding detection and quantification of magnetically labeled cells with various MRI contrast agents with a focus on SPIO nanoparticles...
  28. ncbi Histological analysis of GFP expression in murine bone
    Xi Jiang
    Department of Genetics and Development Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Histochem Cytochem 53:593-602. 2005
    ..The same imaging approach can serve as a vehicle for archiving, retrieving, and sharing histological images among research groups...
  29. ncbi Fountain Flow cytometry, a new technique for the rapid detection and enumeration of microorganisms in aqueous samples
    Paul E Johnson
    Department of Physics and Astronomy, University of Wyoming, Laramie, Wyoming 82070, USA
    Cytometry A 69:1212-21. 2006
    ..There is an urgent need to develop a technique for the real-time detection of pathogens in environmental samples at low concentrations, <10 microorganisms/ml, in large sample volumes, > or =100 ml...
  30. ncbi Standardization and optimization of multiparameter intracellular cytokine staining
    Laurel Nomura
    BD Biosciences, 2350 Qume Dr, San Jose, California 95131, USA
    Cytometry A 73:984-91. 2008
    ..We present data from two different ongoing multi-laboratory standardization studies, one involving 15 laboratories and one involving nine. We identify issues of variability and, where possible, offer solutions...
  31. ncbi Long-term in vivo imaging of β-amyloid plaque appearance and growth in a mouse model of cerebral β-amyloidosis
    Jasmin K Hefendehl
    Department of Cellular Neurology, Hertie Institute for Clinical Brain Research, University of Tubingen, Tubingen, Germany
    J Neurosci 31:624-9. 2011
    ..A solid knowledge of the dynamics of cerebral β-amyloidosis in mouse models provides a powerful tool to monitor preclinical Aβ targeting therapeutic strategies and eases the interpretation of diagnostic amyloid imaging in humans...
  32. ncbi Cytotoxic effects of iron oxide nanoparticles and implications for safety in cell labelling
    Stefaan J H Soenen
    Lab of BioNanoColloids, Interdisciplinary Research Centre, Katholieke Universiteit Leuven, Campus Kortrijk, B8500 Kortrijk, Belgium
    Biomaterials 32:195-205. 2011
    ..These results highlight the importance of in-depth cytotoxic evaluation of cell labelling studies as at non-toxic concentrations, some particles appear to be less suitable for the MR visualization of labelled cells...
  33. ncbi Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots
    Xingyong Wu
    Quantum Dot Corporation, 26118 Research Rd, Hayward, CA 94545, USA
    Nat Biotechnol 21:41-6. 2003
    ..The results indicate that QD-based probes can be very effective in cellular imaging and offer substantial advantages over organic dyes in multiplex target detection...
  34. ncbi A 96-well plate fluorescence assay for assessment of cellular permeability and active efflux in Salmonella enterica serovar Typhimurium and Escherichia coli
    Nick G Coldham
    Department of Food and Environmental Safety, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK
    J Antimicrob Chemother 65:1655-63. 2010
    ..Porin loss and/or active efflux have been identified as a key mechanisms of MAR. A single rapid test was developed for MAR...
  35. ncbi Infarct volume quantification in mouse focal cerebral ischemia: a comparison of triphenyltetrazolium chloride and cresyl violet staining techniques
    Kudret Tureyen
    Department of Neurological Surgery, University of Wisconsin Madison, K4 822 Clinical Science Center, 600 Highland Avenue, Madison, WI 53792, USA
    J Neurosci Methods 139:203-7. 2004
    ..05). TTC and CV staining showed a high degree of correlation in infarct area and volume indicating that both methods are suitable for producing accurate measurements of cerebral experimental infarcts...
  36. pmc High-efficiency labeling of sialylated glycoproteins on living cells
    Ying Zeng
    Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    Nat Methods 6:207-9. 2009
    ..Aniline catalysis dramatically accelerates oxime ligation, allowing use of low concentrations of aminooxy-biotin at neutral pH to label the majority of cell-surface sialylated glycoproteins while maintaining high cell viability...
  37. pmc Functional recovery of stepping in rats after a complete neonatal spinal cord transection is not due to regrowth across the lesion site
    N J K Tillakaratne
    Department of Physiological Science, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Neuroscience 166:23-33. 2010
  38. ncbi Detection of small differences in actomyosin function using actin labeled with different phalloidin conjugates
    Martina Balaz
    Department of Chemistry and Biomedical Sciences, University of Kalmar, SE 391 82 Kalmar, Sweden
    Anal Biochem 338:224-36. 2005
    ..We discuss implications of the results for nanotechnological applications, understanding actomyosin function, and reducing experimental costs and the use of laboratory animals...
  39. pmc A combined histological and MRI brain atlas of the common marmoset monkey, Callithrix jacchus
    John D Newman
    Unit on Developmental Neuroethology, Laboratory of Comparative Ethology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Poolesville, MD 20837, USA
    Brain Res Rev 62:1-18. 2009
    ..Naming of identifiable brain structures was performed utilizing current terminology. The histological sections and a simplified schematic atlas are available online at
  40. pmc Association of aldehyde dehydrogenase 2 gene polymorphism with multiple oesophageal dysplasia in head and neck cancer patients
    M Muto
    Department of Gastrointestinal Oncology and Gastroenterology, National Cancer Center Hospital East, Kashiwa, Japan
    Gut 47:256-61. 2000
    ..Acetaldehyde, the first metabolite of ethanol, is thought to play an important role in the carcinogenesis of the upper aerodigestive tract...
  41. ncbi Magnesium hydroxide temporarily enhancing osteoblast activity and decreasing the osteoclast number in peri-implant bone remodelling
    C Janning
    Laboratory for Biomechanics and Biomaterials, Department of Orthopaedic Surgery, Hannover Medical School, Anna von Borries Strasse 1 7, 30625 Hannover, Germany
    Acta Biomater 6:1861-8. 2010
    ..Further studies have to evaluate if the enhanced bone growth is mainly due to the local magnesium ion concentration or the local alkalosis accompanying the Mg(OH)(2) dissolution...
  42. ncbi von Kossa staining alone is not sufficient to confirm that mineralization in vitro represents bone formation
    L F Bonewald
    Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78284 7877, USA
    Calcif Tissue Int 72:537-47. 2003
  43. pmc Bioorthogonal chemistry: fishing for selectivity in a sea of functionality
    Ellen M Sletten
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Angew Chem Int Ed Engl 48:6974-98. 2009
    ..Herein we describe the bioorthogonal chemical reactions developed to date and how they can be used to study biomolecules...
  44. pmc Analysis of prototype foamy virus particle-host cell interaction with autofluorescent retroviral particles
    Kristin Stirnnagel
    Institut fur Virologie, Medizinische Fakultät Carl Gustav Carus, Technische Universitat Dresden, Dresden, Germany
    Retrovirology 7:45. 2010
    ..Second, the unusually broad host range of FV is thought to be due to use of a very common entry receptor present on host cell plasma membranes, because all cell lines tested in vitro so far are permissive...
  45. ncbi Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity
    D Wendt
    Departments of Surgery and of Research, University Hospital Basel, Hebelstrasse 20, ZLF, Room 405, 4031 Basel, Switzerland
    Biotechnol Bioeng 84:205-14. 2003
  46. ncbi A novel method to determine specificity and sensitivity of the TUNEL reaction in the quantitation of apoptosis
    K J Kelly
    Department of Medicine, Division of Nephrology, Indiana Center for Biological Microscopy, Indiana University, Indianapolis, Indiana 46202, USA
    Am J Physiol Cell Physiol 284:C1309-18. 2003
    ..This novel and simple method will permit the determination of indices of sensitivity and specificity for the TUNEL assay in other tissues and experimental conditions...
  47. ncbi Cell death and proliferation in acute slices and organotypic cultures of mammalian CNS
    Laura Lossi
    Dipartimento di Morfofisiologia Veterinaria, University of Turin, Turin, Italy
    Prog Neurobiol 88:221-45. 2009
    ..e. the cortical, hippocampal, cerebellar and retinal slices...
  48. pmc Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD
    Onur Kanisicak
    Department of Molecular and Cell Biology, Center for Regenerative Biology, University of Connecticut Stem Cell Institute, University of Connecticut, Storrs, CT 06269, USA
    Dev Biol 332:131-41. 2009
    ..The results suggest that satellite cells are derived from committed myogenic progenitors, irrespective of the anatomical location, embryological origin, or physiological properties of associated musculature...
  49. pmc In vitro and in vivo high-throughput assays for the testing of anti-Trypanosoma cruzi compounds
    Adriana M C Canavaci
    Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, United States of America
    PLoS Negl Trop Dis 4:e740. 2010
    ..With scant economic resources available for antiparasitic drug discovery and development, inexpensive, high-throughput and in vivo assays to screen potential new drugs and existing compound libraries are essential...
  50. ncbi Mitochondria directly influence fertilisation outcome in the pig
    Shahinaz H El Shourbagy
    The Mitochondrial and Reproductive Genetics Group, The Medical School, The University of Birmingham, Birmingham B15 2TT, UK
    Reproduction 131:233-45. 2006
  51. ncbi Labeling of human mesenchymal stromal cells with superparamagnetic iron oxide leads to a decrease in migration capacity and colony formation ability
    Richard SchAfer
    Institute of Clinical and Experimental Transfusion Medicine, University Hospital Tubingen, Germany
    Cytotherapy 11:68-78. 2009
  52. ncbi Comparison of SPIO and USPIO for in vitro labeling of human monocytes: MR detection and cell function
    Raoul D Oude Engberink
    Image Sciences Institute, University Medical Center Utrecht, Utrecht, The Netherlands
    Radiology 243:467-74. 2007
  53. pmc Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes
    Maddalena Fratelli
    Mario Negri Institute for Pharmacological Research, 20157 Milan, Italy
    Proc Natl Acad Sci U S A 99:3505-10. 2002
    ..These findings suggest that protein glutathionylation might be a common mechanism for the global regulation of protein functions...
  54. ncbi Optimized lymphocyte isolation methods for analysis of chemokine receptor expression
    Della Berhanu
    Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol Methods 279:199-207. 2003
    ..However, the precise method used will depend on which receptor is being measured. We used the optimal methods to compare the expression of chemokine receptors on naive and memory T-cell subsets using 8-color flow cytometry...
  55. ncbi A twist code determines the onset of osteoblast differentiation
    Peter Bialek
    Department of Molecular and Human Genetics, Bone Disease Program of Texas, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Dev Cell 6:423-35. 2004
    ..In vivo mutagenesis confirms the antiosteogenic function of the Twist box. Thus, relief of inhibition by Twist proteins is a mandatory event precluding osteoblast differentiation...
  56. pmc Real-time passive volume responses of astrocytes to acute osmotic and ischemic stress in cortical slices and in vivo revealed by two-photon microscopy
    W Christopher Risher
    Graduate Program in Neuroscience, Medical College of Georgia, Augusta, Georgia
    Glia 57:207-21. 2009
    ..Unlike neurons, astrocytes better recover from brief ischemic insult in cortical slices, probably because their aquaporins facilitate water efflux...
  57. pmc Cell-type-specific excitatory and inhibitory circuits involving primary afferents in the substantia gelatinosa of the rat spinal dorsal horn in vitro
    Toshiharu Yasaka
    Department of Integrative Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812 8582, Japan
    J Physiol 581:603-18. 2007
    ..These results make it possible to identify primary afferent circuits associated with particular types of SG neurone...
  58. ncbi Site-specific chemical labeling of long RNA molecules
    Kasper Jahn
    Danish National Research Foundation, Center for DNA Nanotechnology at Interdisciplinary Nanoscience Center, Aarhus University, DK 8000 Aarhus, Denmark
    Bioconjug Chem 22:95-100. 2011
    ..The method is rapid and allows site specific labeling of both in vitro and in vivo synthesized RNA with a broad range of functional groups...
  59. pmc Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy
    Guido M Gaietta
    National Center for Microscopy and Imaging Research, Center for Research on Biological Structure, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:17777-82. 2006
    ..Our studies provide previously undescribed insights into Golgi disassociation and reassembly during mitosis and offer a powerful approach to follow recombinant protein distribution in 4D imaging and correlated high-resolution analysis...
  60. ncbi Brilliant blue G selectively stains the internal limiting membrane/brilliant blue G-assisted membrane peeling
    Hiroshi Enaida
    Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Retina 26:631-6. 2006
    ..To report the use of the dye brilliant blue G (BBG) for staining of the internal limiting membrane (ILM) during macular hole (MH) and epiretinal membrane (ERM) surgery...
  61. ncbi Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging
    Zhenhua Ren
    Cell Therapy Center, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China
    Sci China Life Sci 54:981-7. 2011
    ..Our method is helpful for detection of transplanted stem cells in vivo, which is required for understanding mechanisms of cell therapy...
  62. ncbi Quantitative interaction proteomics using mass spectrometry
    Alexander Wepf
    Institute of Molecular Systems Biology, Swiss Federal Institute of Technology, Eidgenossische Technische Hochschule Zurich, Wolfgang Pauli Strasse 16, Zurich, Switzerland
    Nat Methods 6:203-5. 2009
    ..We used this method to quantitatively analyze interaction stoichiometries in the human protein phosphatase 2A network...
  63. pmc Vascular smooth muscle cells ablation with endovascular nonthermal irreversible electroporation
    Elad Maor
    Biophysics Graduate Group, Department of Mechanical Engineering, 6124 Etcheverry Hall, University of California, Berkeley, CA 94720, USA
    J Vasc Interv Radiol 21:1708-15. 2010
    ..To evaluate the effect of endovascular nonthermal irreversible electroporation (NTIRE) on blood vessels...
  64. ncbi In vivo magnetic resonance tracking of magnetically labeled cells after transplantation
    Jeff W M Bulte
    Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2195, USA
    J Cereb Blood Flow Metab 22:899-907. 2002
    ..Now that MR tracking of magnetically labeled cells appears feasible, it is anticipated that this technique may ultimately become an important tool for monitoring the efficacy of clinical (stem) cell transplantation protocols...
  65. ncbi Non-acid-fastness in Mycobacterium tuberculosis ΔkasB mutant correlates with the cell envelope electron density
    Hiroyuki Yamada
    Department of Mycobacterium Reference and Research, The Research Institute of Tuberculosis, Japan Anti Tuberculosis Association, 3 1 24, Matsuyama, Kiyose, Tokyo 204 8533, Japan
    Tuberculosis (Edinb) 92:351-7. 2012
  66. pmc Bioluminescence imaging to track bacterial dissemination of Yersinia pestis using different routes of infection in mice
    Rodrigo J Gonzalez
    Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC, USA
    BMC Microbiol 12:147. 2012
    ..Fully virulent Y. pestis was transformed with a plasmid containing the luxCDABE genes, making it able to produce light; this lux-expressing strain was used to infect mice by subcutaneous, intradermal or intranasal inoculation...
  67. pmc Age-related adaptation of bone-PDL-tooth complex: Rattus-Norvegicus as a model system
    Narita L Leong
    Division of Biomaterials and Bioengineering, University of California San Francisco, San Francisco, California, USA
    PLoS ONE 7:e35980. 2012
    ..5 to 4 months), growth (4 to 10 months), followed by a senescent phase (10 to 15 months), from which deviations due to experimentally induced perturbations can be effectively investigated...
  68. ncbi The application of cell-based label-free technology in drug discovery
    Biao Xi
    ACEA Biosciences, San Diego, CA 92121, USA
    Biotechnol J 3:484-95. 2008
    ..In this article, we have reviewed the various label-free technologies that are being used in drug discovery settings and have focused our discussion on impedance-based label-free technologies and its main applications in drug discovery...
  69. ncbi Surface trafficking of neurotransmitter receptor: comparison between single-molecule/quantum dot strategies
    Laurent Groc
    Physiologie Cellulaire de la Synapse, Unité Mixte de Recherche 5091, Centre National de la Recherche Scientifique, Universite Bordeaux 2, 33077 Bordeaux, France
    J Neurosci 27:12433-7. 2007
  70. ncbi Spatiotemporal expression of Wnt5a during the development of the hindgut and anorectum in human embryos
    Fei Fei Li
    Department of Pediatric Surgery, Shengjing Hospital, China Medical University, No 36 Sanhao Street, Heping District, Shenyang, People s Republic of China, 110004
    Int J Colorectal Dis 26:983-8. 2011
    ..The aim of the study was to determine the spatiotemporal expression of Wnt5a during hindgut and anorectum development in human embryos and to explore the possible role of Wnt5a during the morphogenesis of the human hindgut and anorectum...
  71. ncbi The use of oligoperoxide-coated magnetic nanoparticles to label stem cells
    Daniela Sponarová
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 162 06 Prague 6, Czech Republic
    J Biomed Nanotechnol 7:384-94. 2011
    ..Such particles are thus promising for in vivo MR imaging of transplanted cells. Moreover, PVBM offers the possibility of additional modification by grafting compounds that reduce non-specific protein adsorption...
  72. pmc Magnetic resonance imaging of chondrocytes labeled with superparamagnetic iron oxide nanoparticles in tissue-engineered cartilage
    Sharan Ramaswamy
    Magnetic Resonance Imaging and Spectroscopy Section, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Tissue Eng Part A 15:3899-910. 2009
    ..We believe that this method of visualizing and tracking chondrocytes may be useful in the further development of tissue engineered cartilage therapeutics...
  73. ncbi Spectral phasor analysis allows rapid and reliable unmixing of fluorescence microscopy spectral images
    Farzad Fereidouni
    Department of Molecular Biophysics, Debye Institute, Utrecht University, Utrecht, The Netherlands
    Opt Express 20:12729-41. 2012
    ..In addition the method is used to analyse autofluorescence of cells in a fresh grass blade. It is shown that the spectral phasor approach is compatible with spectral imaging data recorded with a low number of spectral channels...
  74. ncbi Hepatitis C virus expressing flag-tagged envelope protein 2 has unaltered infectivity and density, is specifically neutralized by flag antibodies and can be purified by affinity chromatography
    Jannick Prentoe
    Copenhagen Hepatitis C Program CO HEP, Department of Infectious Diseases and Clinical Research Centre, Copenhagen University Hospital, Hvidovre, Denmark
    Virology 409:148-55. 2011
    ..The full viability and unaltered physiochemical properties of flag-tagged HCV is an important improvement for utilizing these viruses for imaging, virion composition analysis and possibly vaccine development...
  75. ncbi Dual in vivo magnetic resonance evaluation of magnetically labeled mouse embryonic stem cells and cardiac function at 1.5 t
    Takayasu Arai
    Division of Cardiovascular Medicine, Stanford University, California 94305 5233, USA
    Magn Reson Med 55:203-9. 2006
    ..Thus, in vivo MRI enables simultaneous detection of transplanted mESC and their therapeutic effect on the injured myocardium...
  76. pmc Fluorescence-based fixative and vital staining of lipid droplets in Caenorhabditis elegans reveal fat stores using microscopy and flow cytometry approaches
    Maja Klapper
    Institute of Human Nutrition and Food Science, Research Group Molecular Prevention, University of Kiel, Kiel, Germany
    J Lipid Res 52:1281-93. 2011
    ..Our approaches enable sensitive, accurate, and high-throughput assessment of adiposity in large C. elegans populations at a single-worm level...
  77. ncbi Red but not dead? Membranes of stressed Saccharomyces cerevisiae are permeable to propidium iodide
    H M Davey
    Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais, Aberystwyth, UK
    Environ Microbiol 13:163-71. 2011
    ..We discuss these findings in the context of the potential for such environmental stress-induced, transient membrane permeability to have evolutionary implications via the facilitation of horizontal gene transfer...
  78. ncbi A microtiter-plate screening method for biofilm disinfection and removal
    Betsey Pitts
    Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA
    J Microbiol Methods 54:269-76. 2003
    ..Using these assays, agents can be ranked by their ability to remove or kill biofilm...
  79. pmc Dihydroisoxazole analogs for labeling and visualization of catalytically active transglutaminase 2
    Laila Dafik
    Departments of Chemistry and Chemical Engineering, Stanford University, Stanford, CA 94305, USA
    Chem Biol 18:58-66. 2011
    ..The potential use of these inhibitors to probe the role of TG2 in celiac sprue as well as other diseases is discussed...
  80. pmc Metabolic labeling of fucosylated glycans in developing zebrafish
    Karen W Dehnert
    Department of Chemistry, and Howard Hughes Medical Institute, University of California Berkeley, CA 94720, United States
    ACS Chem Biol 6:547-52. 2011
    ..We used this technique to image fucosylated glycans in the enveloping layer of zebrafish embryos during the first 5 days of development. This work provides a method to study the biosynthesis of fucosylated glycans in vivo...
  81. ncbi Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro
    Antje Keppler
    Institute of Molecular and Biological Chemistry, Ecole Polytechnique Federale de Lausanne, Lausanne CH 1015, Switzerland
    Methods 32:437-44. 2004
    ..Here, we report optimized protocols for the synthesis of O6-benzylguanine derivatives and the use of such derivatives for the labeling of different hAGT fusion proteins in vivo and in vitro...
  82. ncbi Multiplex coherent anti-stokes Raman spectroscopy images intact atheromatous lesions and concomitantly identifies distinct chemical profiles of atherosclerotic lipids
    Se Hwa Kim
    Center for Nano Bio Technology, Korea Research Institute of Standards and Science, 1 Doryong dong, Yuseong Gu, Daejeon 305 340, Republic of Korea
    Circ Res 106:1332-41. 2010
    ..However, their physiological role on the progression of atherosclerosis including plaque vulnerability has not been clearly understood, because of the lack of appropriate tools for chemical assessment...
  83. ncbi Superparamagnetic iron oxide nanoparticles may affect endothelial progenitor cell migration ability and adhesion capacity
    Jin Xiu Yang
    Department of Cardiology, The First Affiliated Hospital, Medical School of Zhejiang University, Hangzhou, China
    Cytotherapy 12:251-9. 2010
  84. ncbi Sorting of Leishmania-bearing dendritic cells reveals subtle parasite-induced modulation of host-cell gene expression
    Hervé Lecoeur
    Institut Pasteur, Unité d Immunophysiologie et Parasitisme Intracellulaire, Département de Parasitologie et Mycologie, Paris Cedex 15, France
    Microbes Infect 12:46-54. 2010
    ..In conclusion, this approach provides a powerful method for further characterisation, of amastigote-hosting DCs in the skin and the skin-draining lymph nodes...
  85. ncbi In vivo intracellular recording suggests that gray matter astrocytes in mature cerebral cortex and hippocampus are electrophysiologically homogeneous
    Tsuneko Mishima
    Hirase Research Unit, RIKEN Brain Science Institute, Wako, Japan mail
    J Neurosci 30:3093-100. 2010
    ..We attribute the variability of the membrane potential fluctuations to local potassium concentration changes due to neuronal activity...
  86. ncbi Site-specific labeling of proteins with small molecules in live cells
    Irwin Chen
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Curr Opin Biotechnol 16:35-40. 2005
  87. pmc Controls for immunocytochemistry: an update
    Richard W Burry
    Department of Neuroscience, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Histochem Cytochem 59:6-12. 2011
    ..Publications containing immunocytochemical results must give details of how these controls were performed...
  88. pmc An image-based high-content screening assay for compounds targeting intracellular Leishmania donovani amastigotes in human macrophages
    Jair L Siqueira-Neto
    Center for Neglected Diseases Drug Discovery CND3, Institut Pasteur Korea, Seongnam si, Gyeonggi Do, South Korea
    PLoS Negl Trop Dis 6:e1671. 2012
    ..These data corroborate the idea that the intracellular amastigote form of the parasite is the most appropriate to be used in primary screening assay for Leishmania...
  89. pmc New endoscopic approaches in IBD
    Helmut Neumann
    Department of Medicine I, University of Erlangen Nuremberg, 91054 Erlangen, Germany
    World J Gastroenterol 17:63-8. 2011
    ..This review offers an overview about new endoscopic techniques, including chromoendoscopy, magnification endoscopy, spectroscopy, confocal laser endomicroscopy and endocytoscopy in the face of IBD...
  90. pmc Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging
    - Jasmin
    Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    J Nanobiotechnology 9:4. 2011
    ..In this study we performed experiments designed to investigate the biological properties, including proliferation, viability and differentiation capacity of mesenchymal cells (MSCs) labeled with clinically approved SPIONs...
  91. ncbi 5-Ethynyl-2'-deoxycytidine as a new agent for DNA labeling: detection of proliferating cells
    Dezhong Qu
    Laboratory of RNA Chemical Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Anal Biochem 417:112-21. 2011
    ..Furthermore, the EdC-induced cytotoxicity is much less than that of EdU when combined with thymidine. This will be a potential application for the long-term detection of proliferating cells...
  92. ncbi Effects of iron oxide incorporation for long term cell tracking on MSC differentiation in vitro and in vivo
    Eric Farrell
    Department of Orthopaedics, Erasmus MC, University Medical Centre, Room Ee1651a, Dr Molewaterplein 50, 3015GE Rotterdam, The Netherlands
    Biochem Biophys Res Commun 369:1076-81. 2008
    ..This study demonstrates long term MSC traceability using SPIO and MRI, uninhibited multilineage MSC differentiation following SPIO labeling, though with subtle but significant phenotypical alterations...
  93. ncbi Ferumoxides-protamine sulfate is more effective than ferucarbotran for cell labeling: implications for clinically applicable cell tracking using MRI
    G M van Buul
    Department of Radiology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Contrast Media Mol Imaging 4:230-6. 2009
    ..Consequently, hBMSC and chondrocyte labeling with ferumoxides-protamine is more effective and results in more specific cell labeling than ferucarbotran...
  94. pmc Visualizing antigen-specific and infected cells in situ predicts outcomes in early viral infection
    Qingsheng Li
    Department of Microbiology, Medical School, University of Minnesota, Minneapolis, MN 55455, USA
    Science 323:1726-9. 2009
    ..This method highlights the importance of the location, timing, and magnitude of the immune response needed for a vaccine to be effective against agents of persistent infection, such as HIV-1...
  95. ncbi Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting
    G Nebe-von-Caron
    Unilever Research Colworth, Bedfordshire, MK44 1LQ, Sharnbrook, UK
    J Microbiol Methods 42:97-114. 2000
    ..The detail and precision of multiparameter flow cytometric measurements of cell function at the single cell level now raise questions regarding the validity of classical, growth dependent viability assessment methods...
  96. ncbi Synaptic inputs to two types of koniocellular pathway ganglion cells in marmoset retina
    Kumiko A Percival
    Department of Ophthalmology, Save Sight Institute, University of Sydney, Australia
    J Comp Neurol 519:2135-53. 2011
    ..This suggests functional differences between koniocellular, parvocellular, and magnocellular pathways do not arise from variation in synaptic input densities...
  97. ncbi Vital dyes and light sources for chromovitrectomy: comparative assessment of osmolarity, pH, and spectrophotometry
    Elaine de Paula Fiod Costa
    Department of Ophthalmology, Vision Institute IPEPO, Federal University of Sao Paulo, Sao Paulo, Brazil
    Invest Ophthalmol Vis Sci 50:385-91. 2009
    ..To investigate the in vitro pH, osmolarity, spectral, and photostability properties of nine vital dyes for vitreoretinal surgery...
  98. ncbi Stable long-term intracellular labelling with fluorescently tagged cationic magnetoliposomes
    Stefaan J H Soenen
    Subfaculty of Medicine, Katholieke Universiteit Leuven, Interdisciplinary Research Centre, KUL Campus Kortrijk, E Sabbelaan 53, 8500 Kortrijk, Belgium
    Chembiochem 10:257-67. 2009
  99. ncbi Acridine Orange for malaria diagnosis: its diagnostic performance, its promotion and implementation in Tanzania, and the implications for malaria control
    J Keiser
    Office of Population Research, Princeton University, Princeton, NJ 08544, USA
    Ann Trop Med Parasitol 96:643-54. 2002
    ..The results of this introduction, which are reviewed here and have several important implications, indicate that AO is a viable alternative technique for the laboratory diagnosis of malaria in highly endemic countries...
  100. ncbi Digital stain separation for histological images
    P J Tadrous
    Department of Cellular Pathology, Northwick Park Hospital, Middlesex, England, UK
    J Microsc 240:164-72. 2010
    ..The present method of digital stain separation is fully automatic with no such prerequisites...
  101. pmc SIMPLE: a sequential immunoperoxidase labeling and erasing method
    George Glass
    University of Virginia, UVA Health System, Charlottesville, VA 22908, USA
    J Histochem Cytochem 57:899-905. 2009
    ..SIMPLE is greatly facilitated by the use of a whole-slide scanner, which can capture the results of each sequential stain without any information loss...

Research Grants78

  1. Caspases, Calpains and Neuronal Cell death
    Stan Krajewski; Fiscal Year: 2006
    ..These animals and cells derived from them will then be used for determining the importance of these proteases for neuronal cell death induced by cerebral ischemia and in certain neurodegenerative diseases. ..
    Dara Kraitchman; Fiscal Year: 2004
    ..abstract_text> ..
  3. 2nd Annual Glycoconjugate Analysis Workshop
    Vernon Reinhold; Fiscal Year: 2007
    ..This second workshop will precede the Carbohydrate Gordon Conference held in Tilton, NH a few miles north of the University of New Hampshire. [unreadable] [unreadable] [unreadable]..
  4. Mulago Inpatient Noninvasive Diagnosis of Pneumocystis Pneumonia (MIND PCP) Study
    John Davis; Fiscal Year: 2007
    ..This study will test if DNA- fingerprinting of special mouthwashes can accurately diagnose HIV-related pneumonia, and save lives. [unreadable] [unreadable] [unreadable]..
  5. In Vivo Measurement of Optic Nerve Axon Loss
    JAMES LINDSEY; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  6. Acting Based Regulation of Smooth Muscle Contraction
    JOSEPH CHALOVICH; Fiscal Year: 2007
    ..Heavy emphasis will be placed on this activity and its reversal by Cacalmodulin. These studies will be conducted both in solution and in smooth muscle fiber systems. [unreadable] [unreadable]..
  7. Scanning Electron Microscope for Core EM Facility
    Roger Craig; Fiscal Year: 2007
    ..Many have a direct bearing on the understanding of human disease. Results provided by this instrument will play a key role in advancing our knowledge in these areas. [unreadable] [unreadable] [unreadable]..
  8. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2007
    ..Novelty: This proposal presents a new insight on peroxide-induced iron signaling in endothelial apoptosis and on the role of NO and antioxidants in mitigating these effects. ..
  9. Glyconjugate Analysis Workshop
    Vernon Reinhold; Fiscal Year: 2006
    ..From these discussions, it is hoped that a comprehensive strategy for carbohydrate sequencing can be formulated. [unreadable] [unreadable] [unreadable]..
  10. Genetics of Diabetic Nephropathy in Mexican-Americans a*
    Susanne Nicholas; Fiscal Year: 2006
    ..The final year of the project will focus more heavily on manuscript preparation. Dr. Nicholas will be directly involved in all aspects of the project. ..
  11. Retrograde Dystrophic Influence of IFN-gamma on Neurons
    Pamela Lein; Fiscal Year: 2006
    ..abstract_text> ..
  12. Rational Design of HIV Fusion Inhibitors Targeting gp41
    Shibo Jiang; Fiscal Year: 2010
    ..Therefore, this research is relevant to public health. ..
  13. Enzymatic Metallography for Ultrasensitive Biodetection
    Richard Powell; Fiscal Year: 2006
    ..abstract_text> ..
  14. The Contact Lens and Dry Eye Study
    Jason Nichols; Fiscal Year: 2006
    ..The long-term objective of this research is to contribute to a better understanding of the etiology and risk factors for contact lens-related dry eye so that progress can be made toward its prevention. ..
  15. Methionine Recycling Pathways in Klebsiella
    Jeffrey Lawrence; Fiscal Year: 2006
    ..In this way, characterization of these pathways sheds light on the evolution of complex metabolism, and on the selective forces that lead to gene acquisition, gene loss, and genome change. ..
  16. Bicarbonate Enhances Peroxidation of SOD/ALS Mutants
    Balaraman Kalyanaraman; Fiscal Year: 2008
  17. Membrane Organization in Cell Signaling and Adhesion
    Tione Buranda; Fiscal Year: 2008
    ..Completion of the proposed work will yield a better mechanistic grasp of the dynamics of membrane organization as a factor in signaling and cell adhesion. ..
  18. HIV-1 Fusion Coreceptors
    Christopher Broder; Fiscal Year: 2008
    ..abstract_text> ..
  19. Cellular MRI in Glioma and Radiation Necrosis
    Ali Syed Arbab; Fiscal Year: 2010
    ..Early detection of recurrent or metastatic glioma as well as early differentiation of glioma from radiation necrosis will help clinician to tackle the devastating neurological disease. ..
  20. Biomarkers in Phototherapy of Barrett's Esophagus
    Kenneth K Wang; Fiscal Year: 2010
    ..These studies will provide an improved understanding regarding the response of Barrett's esophagus to ablation therapy. ..
  21. Transplanted Stem Cells and Tumor Progression
    Melissa H Wong; Fiscal Year: 2010
    ..It is feasible that fusion promoting factors, or the fusion products themselves, may be useful targets for preventing tumor progression. ..
  22. Valence Coding and Regulation of Approach-Avoidance in Social and Asocial Species
    James L Goodson; Fiscal Year: 2010
    ..We additionally propose the development of VT RNA interference that will enable us to specifically knock down VT production, thus allowing the functional investigation of specific VT cell groups. ..
  23. SARS-CoV S Protein Receptor-binding Domain-based Vaccines
    Shibo Jiang; Fiscal Year: 2010
    ..The long-term goal of this project is to develop highly effective and safe subunit vaccines for protecting at-risk populations from SARS-CoV infection or bioterrorism attack. ..
  24. Development and Characterization of a Novel Contrast Agent
    Margaret Wheatley; Fiscal Year: 2009
    ..A diagnostic tool that could safely and non-invasively detect tumors at an early stage, before metastasis, would be a great advance in health care. ..
  25. Inferring the Roadmap of Bacterial Gene Transfer
    Jeffrey Lawrence; Fiscal Year: 2009
    ..This framework will allow us to test the hypothesis that gene transfer occurs more often between more closely related lineages. ..
  26. b-catenin's role in establishing the gut stem cell niche
    Melissa Wong; Fiscal Year: 2009
    ..These proposed studies will directly examine the role of Wnt/beta-catenin signaling on maintenance of the stem cell niche and will shed insight into the regulatory control of intestinal stem cells. ..
  27. Mathematical Models of Programmed CTL Proliferation
    Dominik Wodarz; Fiscal Year: 2009
    ..These are the role of effector molecules (such as perform and IFN-g) for CTL dynamics, and the role of CD4 T cell help. The modeling work will be complemented by experiments with VSV and LCMV infected mice. ..
  28. Anti-HIV-1 Composite Cellulose Acetate Phthalate Film
    Shibo Jiang; Fiscal Year: 2008
  29. Differentiation of Glioma from Radiation Injury Using Cellular MRI
    Syed Ali; Fiscal Year: 2008
    ..This magnetic labeling technique will also help investigators to track the injected CTL in the body as well as in the targeted areas by magnetic resonance imaging (MRI). [unreadable] [unreadable] [unreadable]..
  30. Polymeric Enzyme-Gold Probes for Ultrasensitive Protein Blotting
    Richard Powell; Fiscal Year: 2008
    ..This will enable faster, more accurate diagnosis of cancer, infectious diseases and prion diseases, improve therapy selection, and provide new tools for the study of cancer at the molecular level. [unreadable] [unreadable] [unreadable]..
  31. Structure and Function of Tau
    David Eliezer; Fiscal Year: 2008
    ..This work may also have broader implications for understanding and treating other protein aggregation diseases. ..
  32. Novel Method of Surveillance in Barrett's Esophagus
    Kenneth Wang; Fiscal Year: 2008
    ..This is an innovative method by which a physician can diagnose hidden areas of cancer within patients who are at a high risk of developing esophageal cancer. [unreadable] [unreadable] [unreadable]..
  33. Bright Blinking Probes for Fast Multicolor Superresolution Imaging in Live Cells
    Marcel P Bruchez; Fiscal Year: 2010
    ..abstract_text> ..
  34. Correlative Enzymatic and Gold Probes
    Richard Powell; Fiscal Year: 2005
    ..abstract_text> ..
  35. CCD Digital Imaging System for Core EM Facility
    Roger Craig; Fiscal Year: 2004
    ..A third TEM will continue to operate with film for researchers who prefer this medium. ..
  36. 5th Bone Fluid Flow Workshop
    MELISSA KNOTHE TATE; Fiscal Year: 2003
    ..abstract_text> ..
  37. The Osteoblastic Monolayer as a 'Gatekeeper' in Bone
    MELISSA KNOTHE TATE; Fiscal Year: 2003
    ..This study is expected to provide insight into new strategies for treatment of bone diseases such as osteoporosis and osteoarthritis. ..
  38. Reiterative Signal Amplification by Gold Deposition
    Richard Powell; Fiscal Year: 2003
    Richard Powell; Fiscal Year: 2003
    ..After design optimization, it will be ported to an automated slide staining instrument. Once performance is optimized, multi-center clinical trials will be conducted to support application for pre-market approval. ..
    Kenneth Wang; Fiscal Year: 2003
    ..The development of a screening device for Barrett's esophagus that could be operated by paramedical personnel would enable large-scale low cost screening to identify patients at risk for esophageal cancer. ..
  41. A Method To Study Membrane Protein Structure
    Guido Zampighi; Fiscal Year: 2003
  42. Scanning laser Confocal Microscope
    RICHARD BURRY; Fiscal Year: 2003
    ..Acquisition of the Zeiss 510 LSM META will significantly enhance the research programs of the major users and aid other investigators in the university community. ..
  43. TECNIA 12 BioTWIN Transmission Electron Microscope
    Guido Zampighi; Fiscal Year: 2003
    ..abstract_text> ..
  44. Live Cell Correlative Imaging Probes
    Richard Powell; Fiscal Year: 2002
    ..The new probes will be evaluated in the correlative localization of genes, RNA and expressed proteins in the mammalian cell nucleus, and to study the large-scale chromatin structure in the nucleus. ..
    Roger Craig; Fiscal Year: 2002
    ..Preliminary data are presented showing the feasibility of our goals. ..
    Marcel Bruchez; Fiscal Year: 2002
    ..The technology is also directly applicable to many different kinds of tumor analysis and could be used on tissue microarrays to vastly increase the throughput and aid the archiving of tissue section analysis. ..
  47. Pathogenesis of Osteoarthritis in Col2a1 Mutant Mice
    Robert Seegmiller; Fiscal Year: 2002
    ..The overall objective is to establish Dmm/+ mice as an animal model of OA in which various therapies can be tested to determine whether they prevent OA initiation or progression...
    Donald Mock; Fiscal Year: 2001
    ..4) Whether biotin deficiency of similar severity to that observed either early or late in human gestation causes increased rates of fetal malformation in the mouse. 5) The enzyme(s) responsible for biotin beta-oxidation. ..
    William Dowhan; Fiscal Year: 2004
    ..The proposed experiments are expected to define at the molecular level the role of PE and other lipids in the assembly, organization, and function of polytopic membrane proteins. ..
    JOSEPH CHALOVICH; Fiscal Year: 2007
    ..Most importantly, the detailed mathematical analyses may allow us to predict specific interventions to limit the effects of these mutations in afflicted individuals. ..
  51. Cyclic RGD Peptide in the Treatment of DMGS
    Susanne Nicholas; Fiscal Year: 2005
    ..The successful completion of this study can provide a novel and feasible target for treatment in DN. ..
  52. Functional Assessment of Pulmonary Toxicity with MRM
    G Johnson; Fiscal Year: 2005
    ..abstract_text> ..
  53. Methylphenidate & gene expression in the rat brain.
    JOAN BAIZER; Fiscal Year: 2004
    ..abstract_text> ..
  54. Redox control of Nrf2/ARE by thioredoxin and GSH
    Jason Hansen; Fiscal Year: 2005
    ..The roles of GSH and cytosolic and nuclear TRX during NRF2 activation and binding will provide an essential foundation for future developmental toxicology. ..
  55. Correlative Chromogenic Gene and Protein assessment
    Richard Powell; Fiscal Year: 2004
    ..HER2 will be a model for the development of a general method applicable to many cancers and to the future development of other multi-analyte assays and staining procedures. ..
  56. Contrast Enhanced Ultrasound with Therapeutic Modality
    Margaret Wheatley; Fiscal Year: 2004
    ..These studies are a prelude to show feasibility prior to in vivo studies. ..
    Marvin Meistrich; Fiscal Year: 2006
    ..Thus they provide a well-defined model system for study of human infertility. ..
    RICHARD MAGIN; Fiscal Year: 2006
  59. Metabolism of Vitamin A in the Prostate
    Lorraine Gudas; Fiscal Year: 2009
    ..The knowledge generated from these studies should allow us to devise better pharmacological strategies to reverse specific gene repression and to restore more normal retinoid signaling in malignant cells. ..
  60. Optical detection of the molecular processes underlying hippocampal LTP
    Yasunori Hayashi; Fiscal Year: 2010
    ..Itmay be possible to have monkeys perform a task and observe synaptic plasticity in the brain during learning of the task. ..
  61. Becton Dickenson FACSAria Cell Sorter
    John Nolan; Fiscal Year: 2006
    ..The FACSAria plays a key role in the development of an advanced flow cytometry development and application capability at LJBI. [unreadable] [unreadable]..
    Vernon Reinhold; Fiscal Year: 2009
    ..elegans. ..
  63. Structure and Function of Alpha-Synuclein
    David Eliezer; Fiscal Year: 2010
    Vernon Reinhold; Fiscal Year: 2003
    ..Directed and guided by experienced hands in K-12 through graduate science teaching (Hopkins, Bauer, resp.,), this component of our Program may be the most compelling and long lasting. ..
  65. MR-Guided Delivery and Monitoring of Magnetocapsules Immunoprotecting Islet Cells
    JEFF BULTE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  66. Developmental control of cell polarity in vertebrate embryos.
    John B Wallingford; Fiscal Year: 2010
    ..Together, these experiments will help us to understand how PCP signaling influences such diverse cellular processes as convergent extension and ciliogenesis. ..
  67. Flourescent Lifetime Imaging Microscopy (FLIM)
    John Condeelis; Fiscal Year: 2005
    ..As discussed in the budget justification, the system we request will solve the problems of speed, sensitivity, and quantitation for an expert user group. ..
    JAMES JESTER; Fiscal Year: 2003
  69. AOSSM Post-Joint Injury Osteoarthritis Conference
    Constance Chu; Fiscal Year: 2008
    ..This symposium will bring doctors and scientists together to develop research plans to find new methods to prevent this from happening. [unreadable] [unreadable] [unreadable] [unreadable]..
  70. Nuclease-Resistant Aptamers to Botox and Anthrax Toxins
    John Bruno; Fiscal Year: 2004
    ..Such reagents could also be used in environmental biosensors or diagnostic test kits for detection of BOTOX and anthrax toxins in body fluids or environmental samples. ..
    Fredika Robertson; Fiscal Year: 2001
    ..The hypothesis driving this research is that NO and peroxynitrite are mutagenic, and could be important contributors to oxidative DNA damage and mutation in human epidermal keratinocytes at sites of inflammation. ..
    Helen Sherk; Fiscal Year: 2006
    ..The hypothesis is that interruption of transmission of visual cortical information to cerebellum will cause a substantial increase in visually-guided stepping errors. ..
  73. Nuclease-Resistant Aptamers for Anthrax Opsonization
    John Bruno; Fiscal Year: 2004
    ..Such shielded aptamers could even be used in inhalers to enhance alveolar macrophage phagocytosis. ..
    Constance Chu; Fiscal Year: 2002
    ..These studies will also serve to improve understanding of the relative roles of NO and peroxynitrite on the pathophysiology of arthritis. ..
  75. Development of Genetic Tools for Clostridium botulinum
    Eric Johnson; Fiscal Year: 2005
    ..abstract_text> ..
  76. In Vitro Mechanisms of Ethanol Induced Neuronal Death.
    Steven Mennerick; Fiscal Year: 2005
    ..The proposed experiments should lead to a better fundamental understanding of the mechanisms by which forebrain neurons are susceptible to ethanol-induced death. ..
    BEATRICE YUE; Fiscal Year: 2009
    ..The overall goal of our proposal is to illustrate the mechanisms to provide a deeper understanding regarding the pathogenesis of glaucomas. ..