electroporation

Summary

Summary: A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of cell membranes, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA. Some applications of electroporation include introduction of plasmids or foreign DNA into living cells for transfection, fusion of cells to prepare hybridomas, and insertion of proteins into cell membranes.

Top Publications

  1. pmc Electroporation and RNA interference in the rodent retina in vivo and in vitro
    Takahiko Matsuda
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:16-22. 2004
  2. pmc In vivo electroporation enhances the immunogenicity of an HIV-1 DNA vaccine candidate in healthy volunteers
    Sandhya Vasan
    Aaron Diamond AIDS Research Center, New York, New York, United States of America
    PLoS ONE 6:e19252. 2011
  3. pmc Controlled expression of transgenes introduced by in vivo electroporation
    Takahiko Matsuda
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:1027-32. 2007
  4. ncbi Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex
    H Tabata
    Department of Molecular Neurobiology, Institute of DNA Medicine, Jikei University School of Medicine, Minato-ku, 105-8461, Tokyo, Japan
    Neuroscience 103:865-72. 2001
  5. ncbi A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO 80523, USA
    J Microbiol Methods 64:391-7. 2006
  6. pmc Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma
    Adil I Daud
    Cutaneous Oncology and Experimental Therapeutics Programs, H Lee Moffitt Cancer Center, University of South Florida, Tampa, FL, USA
    J Clin Oncol 26:5896-903. 2008
  7. pmc Safety and comparative immunogenicity of an HIV-1 DNA vaccine in combination with plasmid interleukin 12 and impact of intramuscular electroporation for delivery
    Spyros A Kalams
    Infectious Diseases Division, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Infect Dis 208:818-29. 2013
  8. ncbi Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian, Ciona intestinalis
    J C Corbo
    Department of Biology, University of California, San Diego, La Jolla 92093 0347, USA
    Development 124:589-602. 1997
  9. ncbi Efficient gene transfer into the embryonic mouse brain using in vivo electroporation
    T Saito
    Department of Development and Differentiation, Kyoto University, Kyoto, 606 8507, Japan
    Dev Biol 240:237-46. 2001
  10. ncbi Tissue ablation with irreversible electroporation
    R V Davalos
    Microsystems and Advanced Concepts Engineering, Sandia National Laboratories, Livermore, CA 94550, USA
    Ann Biomed Eng 33:223-31. 2005

Detail Information

Publications407 found, 100 shown here

  1. pmc Electroporation and RNA interference in the rodent retina in vivo and in vitro
    Takahiko Matsuda
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:16-22. 2004
    ..Here, we report a rapid and convenient electroporation method for both gain- and loss-of-function studies in vivo and in vitro in the rodent retina...
  2. pmc In vivo electroporation enhances the immunogenicity of an HIV-1 DNA vaccine candidate in healthy volunteers
    Sandhya Vasan
    Aaron Diamond AIDS Research Center, New York, New York, United States of America
    PLoS ONE 6:e19252. 2011
    ..DNA-based vaccines have been safe but weakly immunogenic in humans to date...
  3. pmc Controlled expression of transgenes introduced by in vivo electroporation
    Takahiko Matsuda
    Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:1027-32. 2007
    In vivo electroporation is a powerful technique for the introduction of genes into organisms. Temporal and spatial regulation of expression of introduced genes, or of RNAi, would further enhance the utility of this method...
  4. ncbi Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex
    H Tabata
    Department of Molecular Neurobiology, Institute of DNA Medicine, Jikei University School of Medicine, Minato-ku, 105-8461, Tokyo, Japan
    Neuroscience 103:865-72. 2001
    We report a novel gene transfer system using electroporation. We used this technique to introduce a marker gene plasmid containing enhanced green fluorescent protein into mouse brains at embryonic day 12-17 without removing the embryos ..
  5. ncbi A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO 80523, USA
    J Microbiol Methods 64:391-7. 2006
    ....
  6. pmc Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma
    Adil I Daud
    Cutaneous Oncology and Experimental Therapeutics Programs, H Lee Moffitt Cancer Center, University of South Florida, Tampa, FL, USA
    J Clin Oncol 26:5896-903. 2008
    ..In vivo electroporation, in preclinical models, significantly enhances gene transfer efficiency while retaining the safety advantages ..
  7. pmc Safety and comparative immunogenicity of an HIV-1 DNA vaccine in combination with plasmid interleukin 12 and impact of intramuscular electroporation for delivery
    Spyros A Kalams
    Infectious Diseases Division, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Infect Dis 208:818-29. 2013
    ..DNA vaccines have been very poorly immunogenic in humans but have been an effective priming modality in prime-boost regimens. Methods to increase the immunogenicity of DNA vaccines are needed...
  8. ncbi Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian, Ciona intestinalis
    J C Corbo
    Department of Biology, University of California, San Diego, La Jolla 92093 0347, USA
    Development 124:589-602. 1997
    ..Particular efforts center on the regulation of a notochord-specific Ciona Brachyury gene (Ci-Bra). An electroporation method was devised for the efficient incorporation of transgenic DNA into Ciona embryos...
  9. ncbi Efficient gene transfer into the embryonic mouse brain using in vivo electroporation
    T Saito
    Department of Development and Differentiation, Kyoto University, Kyoto, 606 8507, Japan
    Dev Biol 240:237-46. 2001
    ..developed a novel method to introduce DNA into in/exo utero embryonic mouse brains at various stages by using electroporation. More than 90% of operated embryos survived, and more than 65% of these expressed the introduced genes in ..
  10. ncbi Tissue ablation with irreversible electroporation
    R V Davalos
    Microsystems and Advanced Concepts Engineering, Sandia National Laboratories, Livermore, CA 94550, USA
    Ann Biomed Eng 33:223-31. 2005
    ..for minimally invasive treatment of cancer-the ablation of undesirable tissue through the use of irreversible electroporation. Electroporation is the permeabilization of the cell membrane due to an applied electric field...
  11. pmc Electroporation delivery of DNA vaccines: prospects for success
    Niranjan Y Sardesai
    Inovio Pharmaceuticals, 1787 Sentry Parkway, Blue Bell, PA 19422, United States
    Curr Opin Immunol 23:421-9. 2011
    ..with novel encoded molecular adjuvants; and the improvement in new in vivo delivery strategies including electroporation (EP)...
  12. ncbi Neocortex patterning by the secreted signaling molecule FGF8
    T Fukuchi-Shimogori
    Department of Neurobiology, Pharmacology and Physiology, University of Chicago, Chicago, IL 60637, USA
    Science 294:1071-4. 2001
    ..Using electroporation-mediated gene transfer in mouse embryos, we show that augmenting the endogenous anterior FGF8 signal shifts ..
  13. ncbi Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research
    J Gehl
    Department of Oncology, Herlev Hospital in University of Copenhagen, Herlev, Denmark
    Acta Physiol Scand 177:437-47. 2003
    b>Electroporation designates the use of short high-voltage pulses to overcome the barrier of the cell membrane...
  14. ncbi Induced transmembrane voltage and its correlation with electroporation-mediated molecular transport
    Tadej Kotnik
    Department of Biomedical Engineering, Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, 1000, Ljubljana, Slovenia
    J Membr Biol 236:3-13. 2010
    ..voltage, DeltaPsi (m)) and, for sufficiently strong fields, in a transient increase of membrane permeability (electroporation)...
  15. ncbi Patient-specific treatment planning of electrochemotherapy: procedure design and possible pitfalls
    Denis Pavliha
    University of Ljubljana, Faculty of Electrical Engineering, Ljubljana, Slovenia
    Bioelectrochemistry 87:265-73. 2012
    Electrochemotherapy uses electroporation for enhancing chemotherapy...
  16. ncbi Chemokine-idiotype fusion DNA vaccines are potentiated by bivalency and xenogeneic sequences
    Agnete Brunsvik Fredriksen
    Institute of Immunology, University of Oslo, Oslo, Norway
    Blood 110:1797-805. 2007
    ..When delivered in vivo by intramuscular injection of plasmids followed by electroporation, dimeric proteins efficiently primed APCs in draining lymph nodes for activation and proliferation of Id-..
  17. pmc Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane
    Andrei G Pakhomov
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA
    Biochem Biophys Res Commun 385:181-6. 2009
    Cell permeabilization by electric pulses (EPs), or electroporation, has been well established as a tool to indiscriminately increase membrane flows of water solutes down the concentration and voltage gradients...
  18. ncbi Irreversible electroporation: a new challenge in "out of operating theater" anesthesia
    Christine Ball
    Department of Anaesthesia and Perioperative Medicine, Alfred Hospital, Prahran, Victoria, Australia
    Anesth Analg 110:1305-9. 2010
    ..Pulsed electric current can be used to produce irreversible electroporation (IRE) of cell membranes with resulting cell death...
  19. ncbi Optimization of electroporation-mediated transformation: Staphylococcus carnosus as model organism
    J Löfblom
    Department of Molecular Biotechnology, School of Biotechnology, Royal Institute of Technology KTH, AlbaNova University Center, Stockholm, Sweden
    J Appl Microbiol 102:736-47. 2007
    ..The study was conducted with an aim to optimize the transformation efficiency of the Gram-positive bacterium Staphylococcus carnosus to a level that would enable the creation of cell surface displayed combinatorial protein libraries...
  20. ncbi Irreversible electroporation in medicine
    Boris Rubinsky
    Department of Bioengineering, Graduate Group in Biophysics, University of California at Berkeley, Berkeley, CA 94720, USA
    Technol Cancer Res Treat 6:255-60. 2007
    This is a brief introduction to the emerging field of irreversible electroporation in medicine...
  21. ncbi Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo
    Kazuo Kitamura
    Wolfson Institute for Biomedical Research and Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK
    Nat Methods 5:61-7. 2008
    ..We also show that the method can be used for targeted in vivo single-cell electroporation of plasmid DNA into identified cell types, leading to stable transgene expression...
  22. ncbi Irreversible electroporation: implications for prostate ablation
    Gary Onik
    Center for Bioengineering in the Service of Humanity and Society, School of Engineering and Computer Science, Hebrew University of Jerusalem, Givaat Ram Campus, Jerusalem, Israel 91904
    Technol Cancer Res Treat 6:295-300. 2007
    ..Irreversible electroporation IRE, is a new non-thermal ablation modality that uses short pulses of DC electric current to create ..
  23. ncbi Electroporated DNA vaccine clears away multifocal mammary carcinomas in her-2/neu transgenic mice
    Elena Quaglino
    Department of Clinical and Biological Sciences, University of Turin, Orbassano, Italy
    Cancer Res 64:2858-64. 2004
    ..These findings show that an appropriate vaccine effectively inhibits the progression of multifocal preneoplastic lesions...
  24. pmc Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts
    Jason W Ross
    Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
    Transgenic Res 19:611-20. 2010
    ..GFP vector in surviving cells of three fetal fibroblast cell lines, and (2) to determine the ability of these electroporation parameters to produce stably transfected fibroblast colonies following G418 selection. Cell line (P < 0...
  25. pmc Electroporation-induced electrosensitization
    Olga N Pakhomova
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, United States of America
    PLoS ONE 6:e17100. 2011
    b>Electroporation is a method of disrupting the integrity of cell membrane by electric pulses (EPs). Electrical modeling is widely employed to explain and study electroporation, but even most advanced models show limited predictive power...
  26. ncbi A critical function for beta-amyloid precursor protein in neuronal migration revealed by in utero RNA interference
    Tracy L Young-Pearse
    Center for Neurologic Diseases, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Neurosci 27:14459-69. 2007
    ..We used the method of in utero electroporation of shRNA constructs into the developing cortex to acutely knock down APP in rodents...
  27. ncbi The effect of irreversible electroporation on blood vessels
    Elad Maor
    Department of Biomedical Engineering, Department of Mechanical Engineering, Graduate Program in Biophysics, University of California at Berkeley, Berkeley, CA 94720, USA
    Technol Cancer Res Treat 6:307-12. 2007
    We present a pilot study on the long term effects of irreversible electroporation (IRE) on a large blood vessel. The study was motivated by the anticipated use of IRE for treatment of cancer tumors abutting large blood vessels...
  28. ncbi The use of Chinese hamster ovary (CHO) cells in the study of ion channels
    Nikita Gamper
    Department of Physiology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio TX 78229, USA
    J Pharmacol Toxicol Methods 51:177-85. 2005
    ....
  29. ncbi Safety and early efficacy of irreversible electroporation for hepatic tumors in proximity to vital structures
    Robert Cannon
    Division of Surgical Oncology, Department of Surgery, University of Louisville, Louisville, KY 40202, USA
    J Surg Oncol 107:544-9. 2013
    Irreversible electroporation (IRE) has shown promise for ablation of lesions in proximity to vital structures in the preclinical setting...
  30. ncbi A rapid and simple method for inactivating chromosomal genes in Yersinia
    Anne Derbise
    Unité de bactériologie moléculaire et médicale, Laboratoire des Yersinia, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    FEMS Immunol Med Microbiol 38:113-6. 2003
    ..Using this method, three chromosomal loci were successfully disrupted in Yersinia pseudotuberculosis. The use of this technique allows rapid and efficient large-scale mutagenesis of Yersinia target chromosomal genes...
  31. pmc High-efficiency transformation of Chlamydomonas reinhardtii by electroporation
    K Shimogawara
    Laboratory of Chemistry, Teikyo University School of Medicine, Hachioji, Tokyo, Japan
    Genetics 148:1821-8. 1998
    ..a high-efficiency method for transforming the unicellular, green alga Chlamydomonas reinhardtii by electroporation. Electroporation of strains CC3395 and CC425, cell wall-less mutants devoid of argininosuccinate lyase (..
  32. ncbi Cure of mammary carcinomas in Her-2 transgenic mice through sequential stimulation of innate (neoadjuvant interleukin-12) and adaptive (DNA vaccine electroporation) immunity
    Michela Spadaro
    Department of Clinical and Biological Sciences, University of Turin, Ospedale San Luigi Gonzaga, Regione Gonzole 10, I 10043 Orbassano, Italy
    Clin Cancer Res 11:1941-52. 2005
    ..In a model of Her-2 spontaneous mammary cancer, we investigated the efficacy of neoadjuvant interleukin 12 (IL-12) followed by "immune-surgery" of the residual tumor...
  33. pmc Tumor ablation with irreversible electroporation
    Bassim Al-Sakere
    CNRS UMR 8121, Institut Gustave Roussy, Villejuif, France
    PLoS ONE 2:e1135. 2007
    We report the first successful use of irreversible electroporation for the minimally invasive treatment of aggressive cutaneous tumors implanted in mice...
  34. pmc Electroporation of human microvascular endothelial cells: evidence for an anti-vascular mechanism of electrochemotherapy
    M Cemazar
    Department of Tumor Biology, Institute of Oncology, Zaloska 2, SI 1000, Ljubljana, Slovenia
    Br J Cancer 84:565-70. 2001
    ..of chemotherapeutic drugs with application of high voltage electric pulses applied to the tumour nodule (electroporation), result in a significant reduction in tumour blood flow and may therefore be mediated by an anti-vascular ..
  35. ncbi DNA vaccines increase immunogenicity of idiotypic tumor antigen by targeting novel fusion proteins to antigen-presenting cells
    Agnete B Fredriksen
    Institute of Immunology, University of Oslo, Rikshospitalet and Rikshospitalet University Hospital, Sognsvannsvn 20, 0027 Oslo, Norway
    Mol Ther 13:776-85. 2006
    ..After plasmid injection and electroporation of mouse muscle, secreted vaccine proteins (vaccibodies) delivered idiotypic tumor antigen to APC in draining ..
  36. ncbi Effect of GDNF gene transfer into axotomized retinal ganglion cells using in vivo electroporation with a contact lens-type electrode
    H Ishikawa
    Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Yoshidakonoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Gene Ther 12:289-98. 2005
    We developed an in vivo electroporation method to introduce foreign genes into retinal ganglion cells (RGCs)...
  37. ncbi In vivo electroporation in the embryonic mouse central nervous system
    Tetsuichiro Saito
    Department of Developmental Biology, Graduate School of Medicine, Chiba University, Chiba 260 8670, Japan
    Nat Protoc 1:1552-8. 2006
    This protocol describes a basic method for in vivo electroporation in the nervous system of embryonic mice...
  38. ncbi Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging
    W Aung
    Diagnostic Imaging Group, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan
    Gene Ther 16:830-9. 2009
    In vivo electroporation (EP) is an efficient method for effective gene transfer and is highly expected for application in anticancer gene therapy. Non-invasive monitoring of gene transfer/expression is critical for optimal gene therapy...
  39. ncbi Irreversible electroporation: a new ablation modality--clinical implications
    Boris Rubinsky
    Center for Biomedical Engineering in the Service of Humanity and Society, School of Engineering and Computer Science, Hebrew University of Jerusalem, Givaat Ram Campus, Jerusalem, 91906 Israel
    Technol Cancer Res Treat 6:37-48. 2007
    Irreversible electroporation (IRE) is a new tissue ablation technique in which micro to millisecond electrical pulses are delivered to undesirable tissue to produce cell necrosis through irreversible cell membrane permeabilization...
  40. pmc Overexpression of carnitine palmitoyltransferase-1 in skeletal muscle is sufficient to enhance fatty acid oxidation and improve high-fat diet-induced insulin resistance
    Clinton R Bruce
    Cellular and Molecular Metabolism Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia
    Diabetes 58:550-8. 2009
    ....
  41. ncbi Expression of exogenous genes in Trypanosoma cruzi: improving vectors and electroporation protocols
    Wanderson D DaRocha
    Departamento de Bioquimica e Imunologia, ICB, Universidade Federal de Minas Gerais, MG 31270 010, Belo Horizonte, Brazil
    Parasitol Res 92:113-20. 2004
    To improve transfection efficiency in Trypanosoma cruzi, we developed a new electroporation protocol and expression vectors which use luciferase and green and red fluorescent proteins as reporter genes...
  42. ncbi In vivo results of a new focal tissue ablation technique: irreversible electroporation
    Jon F Edd
    Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA
    IEEE Trans Biomed Eng 53:1409-15. 2006
    ..experiments that confirm the feasibility of a new minimally invasive method for tissue ablation, irreversible electroporation (IRE)...
  43. pmc High-efficiency transfection of primary human and mouse T lymphocytes using RNA electroporation
    Yangbing Zhao
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Ther 13:151-9. 2006
    ..In this report, high gene transfection efficiency with low transfection-related toxicity was achieved by electroporation using in vitro-transcribed mRNA...
  44. ncbi High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol
    Shixuan Wu
    U S Department of Agriculture, Laramie, WY, USA
    Biotechniques 36:152-4. 2004
    ..pastoris with 0.1 M lithium acetate (LiAc) and 10 mM dithiothreitol (DTT) before electroporation increased transformation efficiency approximately 150-fold...
  45. pmc Vascular disrupting action of electroporation and electrochemotherapy with bleomycin in murine sarcoma
    G Sersa
    Institute of Oncology Ljubljana, Zaloska 2, SI 1000 Ljubljana, Slovenia
    Br J Cancer 98:388-98. 2008
    ..model, histological evaluation and physiological measurements of the tumours were carried out to confirm that electroporation and electrochemotherapy of tumours have a vascular disrupting action...
  46. ncbi Imaging guided percutaneous irreversible electroporation: ultrasound and immunohistological correlation
    Edward W Lee
    Department of Radiology, Division of Interventional Radiology, University of California Los Angeles, David Geffen School of Medicine, 10833 Le Conte Avenue, BL 423, Los Angeles, CA 90095 1721, USA
    Technol Cancer Res Treat 6:287-94. 2007
    Preliminary results of percutaneous irreversible electroporation (PIE) on swine liver as a novel non-thermal ablation are presented...
  47. ncbi Combined effects of IL-12 and electroporation enhances the potency of DNA vaccination in macaques
    Lauren A Hirao
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Boulevard, 505 SCL, Philadelphia, PA 19104, United States
    Vaccine 26:3112-20. 2008
    ..IM) injection, DNA with plasmid-encoded IL-12 by IM injection, DNA by IM injection with in vivo electroporation (EP), and DNA with IL-12 by IM EP...
  48. ncbi The endothelial cytoskeleton as a target of electroporation-based therapies
    Chryso Kanthou
    Cancer Research UK Tumour Microcirculation Group, Academic Unit of Surgical Oncology, Royal Hallamshire Hospital, University of Sheffield, UK
    Mol Cancer Ther 5:3145-52. 2006
    b>Electroporation-based therapies, such as electrochemotherapy and electrogene therapy, result in the disruption of blood vessel networks in vivo and cause changes in blood flow and vascular permeability...
  49. ncbi Mechanism of intracellular delivery by acoustic cavitation
    Robyn K Schlicher
    The Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, Georgia 30332 0100, USA
    Ultrasound Med Biol 32:915-24. 2006
    ..In this way, noninvasively focused ultrasound could deliver drugs and genes to targeted tissues, thereby minimizing side effects, lowering drug dosages, and improving efficacy...
  50. ncbi Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cells
    V F Van Tendeloo
    Laboratory of Experimental Hematology, Antwerp University Hospital, University of Antwerp, Antwerp, Belgium
    Blood 98:49-56. 2001
    ..Here, a cytoplasmic expression system based on mRNA electroporation to efficiently introduce tumor antigens into DCs is described...
  51. pmc Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse
    Hehai Wang
    Division of Neonatology, Department of Pediatrics, Vanderbilt University Medical Center, 1125 MRB IV, 2215 B Garland Avenue, Nashville, TN 37232 0656, USA
    Dev Biol 318:112-25. 2008
    ..with this idea, we demonstrate that ZO-1 siRNA delivery inside the blastomeres of zona-weakened embryos using electroporation not only knocks down ZO-1 gene and protein expressions, but also inhibits morula to blastocyst transformation ..
  52. ncbi Stable integration and conditional expression of electroporated transgenes in chicken embryos
    Yuki Sato
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916 5, Takayama, Ikoma, Nara, 630 0192, Japan
    Dev Biol 305:616-24. 2007
    The in ovo electroporation in chicken embryos has widely been used as a powerful tool to study roles of genes during embryogenesis...
  53. pmc Heterosubtypic protection against pathogenic human and avian influenza viruses via in vivo electroporation of synthetic consensus DNA antigens
    Dominick J Laddy
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 3:e2517. 2008
    ..We also evaluated the use of in vivo electroporation to deliver these vaccines to overcome the immunogenicity barrier encountered in larger animal models of ..
  54. pmc Electroporation of synthetic DNA antigens offers protection in nonhuman primates challenged with highly pathogenic avian influenza virus
    Dominick J Laddy
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Virol 83:4624-30. 2009
    ..In this study, by construct optimization and in vivo electroporation of synthetic DNA-encoded antigens, we observed the induction of cross-reactive cellular and humoral immune ..
  55. ncbi Electroporation-mediated topical delivery of vitamin C for cosmetic applications
    L Zhang
    Genetronics Inc, San Diego, CA 92121, USA
    Bioelectrochem Bioenerg 48:453-61. 1999
    ..This report describes the first feasibility study of electroporation-mediated topical delivery (EMTD) of Asc for potential cosmetic applications...
  56. ncbi Nanoparticles for the delivery of genes and drugs to human hepatocytes
    Tadanori Yamada
    Institute of Scientific and Industrial Research, Osaka University, 8 1 Mihogaoka, Ibaraki, Osaka 567 0047, Japan
    Nat Biotechnol 21:885-90. 2003
    ..The yeast-derived L particle is free of viral genomes, highly specific to human liver cells and able to accommodate drugs as well as genes. These advantages should facilitate targeted delivery of genes and drugs to the human liver...
  57. ncbi High antibody and cellular responses induced to HIV-1 clade C envelope following DNA vaccines delivered by electroporation
    Jiangmei Yin
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 SCL, Philadelphia, PA 19104, USA
    Vaccine 29:6763-70. 2011
    ..In this study we tested a DNA based vaccine that encodes the SIVgag, SIVpol and HIV-1 envelope clade C...
  58. ncbi Optimal parameters for the destruction of prostate cancer using irreversible electroporation
    Jonathan Rubinsky
    Department of Mechanical Engineering, University of California Berkeley, Berkeley, California, USA
    J Urol 180:2668-74. 2008
    Irreversible electroporation is a new tissue ablation technique that consists of applying musecond pulses of direct current to create permanent defects in the cell membrane...
  59. ncbi Percutaneous irreversible electroporation (IRE) of hepatic malignant tumours: contrast-enhanced ultrasound (CEUS) findings
    P Wiggermann
    Department of Radiology, University Regensburg Medical Center, Regensburg, Germany
    Clin Hemorheol Microcirc 52:417-27. 2012
    ..aim of this study is to describe the image findings of contrast-enhanced ultrasound (CEUS) after irreversible electroporation (IRE), and to assess its usefulness in relation to the evaluation of the ablation status using a dynamic ..
  60. pmc Dendritic cells loaded with mRNA encoding full-length tumor antigens prime CD4+ and CD8+ T cells in melanoma patients
    An M T Van Nuffel
    Laboratory of Molecular and Cellular Therapy, Department of Immunology Physiology, Medical School of the Vrije Universiteit Brussel VUB, Brussels, Belgium
    Mol Ther 20:1063-74. 2012
    ..Thus, this work provides definitive proof that DCs presenting the full antigenic spectrum of tumor antigens can induce T cells specific for novel epitopes and can be administered to patients irrespective of their HLA type...
  61. ncbi In ovo electroporation of avian somites
    Martin Scaal
    Laboratoire de Genetique et de Physiologie du Developpement, Developmental Biology Institute of Marseille IBDM, CNRS UMR 654, University Aix Marseille II, Campus de Luminy, Marseille, France
    Dev Dyn 229:643-50. 2004
    In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties...
  62. ncbi Irreversible electroporation near the heart: ventricular arrhythmias can be prevented with ECG synchronization
    Ajita Deodhar
    Interventional Radiology and Imaging Guided Therapies, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    AJR Am J Roentgenol 196:W330-5. 2011
    Irreversible electroporation is a nonthermal ablative tool that uses direct electrical pulses to create irreversible membrane pores and cell death...
  63. ncbi Comparison and optimisation of transfection of human dental follicle cells, a novel source of stem cells, with different chemical methods and electro-poration
    Mehmet E Yalvac
    Department of Genetics and Bioengineering, Yeditepe University, 26 Ağustos Campus, Kayisdagi Cad, Kayisdagi, 34755, Istanbul, Turkey
    Neurochem Res 34:1272-7. 2009
    ..However, there are currently no systematic comparative studies on transfection potential of HDFC cells using different chemical and electro-poration techniques...
  64. ncbi Application of in utero electroporation and live imaging in the analyses of neuronal migration during mouse brain development
    Yoshiaki V Nishimura
    Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713 8 Kamiya, Kasugai, Aichi, 480 0392, Japan
    Med Mol Morphol 45:1-6. 2012
    ..The in utero electroporation technique is a powerful method for rapid gain- and loss-of-function studies of neuronal development, ..
  65. ncbi Electropermeabilization, a physical method for the delivery of therapeutic molecules into cells
    Marie Pierre Rols
    Institut de Pharmacologie et de Biologie Structurale du CNRS UMR5089, 205, route de Narbonne, 31077 Toulouse Cedex 4, France
    Biochim Biophys Acta 1758:423-8. 2006
    ..In the case of DNA, transfer occurs through a multi-step mechanism, a process that involves the electrophoretically driven association of the DNA molecule with the destabilised membrane and then its passage...
  66. ncbi Irreversible electroporation therapy in the management of locally advanced pancreatic adenocarcinoma
    Robert C G Martin
    Division of Surgical Oncology, Department of Surgery and James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY 40202, USA
    J Am Coll Surg 215:361-9. 2012
    ..Irreversible electroporation (IRE) is a nonthermal ablation technology that we have shown is safe near vital vascular and ductal ..
  67. ncbi Irreversible electroporation for unresectable hepatocellular carcinoma: initial experience and review of safety and outcomes
    W Cheung
    Department of Radiology, The Alfred Hospital, Melbourne, Victoria, Australia
    Technol Cancer Res Treat 12:233-41. 2013
    The aims of this study were to evaluate the safety, feasibility and tumour response of _irreversible electroporation, a non-thermal ablation technique, for the treatment of unresectable hepatocellular carcinoma...
  68. ncbi Ablation of perivascular hepatic malignant tumors with irreversible electroporation
    T Peter Kingham
    Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Am Coll Surg 215:379-87. 2012
    ..Ablation near portal pedicles and hepatic veins is challenging. Irreversible electroporation (IRE) is a new ablation technique that does not rely on heat and, in animals, appears to be safe and ..
  69. ncbi Direct therapeutic applications of calcium electroporation to effectively induce tumor necrosis
    Stine Krog Frandsen
    Center for Experimental Drug and Gene Electrotransfer, Department of Oncology, Copenhagen University Hospital Herlev, Denmark
    Cancer Res 72:1336-41. 2012
    b>Electroporation of cells with short, high-voltage pulses causes a transient permeabilization of cell membranes that permits passage of otherwise nonpermeating ions and molecules...
  70. pmc The effects of irreversible electroporation (IRE) on nerves
    Wei Li
    Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, The Fourth Military Medical University, Xi an, Shanxi, China
    PLoS ONE 6:e18831. 2011
    ..Though irreversible electroporation (IRE) might be a promising choice to treat tumors near or involved critical nerve, the pathophysiology of the ..
  71. ncbi Coexpression after electroporation of plasmid mixtures into muscle in vivo
    Z A Rana
    Department of Molecular Biosciences, University of Oslo, Blindern, Oslo, Norway
    Acta Physiol Scand 181:233-8. 2004
    ..the most frequently considered tissue for non-viral gene therapy, in particular after gene transfer by electroporation. Expression in muscle is stable, but since the cell turnover is so slow incorporation in the host genome is ..
  72. pmc Electroporation-mediated gene delivery of cleavage-resistant pro-nerve growth factor causes retinal neuro- and vascular degeneration
    Suraporn Matragoon
    Program in Clinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA 30912, USA
    Mol Vis 18:2993-3003. 2012
    ..Since systemic overexpression of proNGF exhibits embryonic lethality, we aimed to establish a model that specifically and stably overexpresses a cleavage-resistant mutant of proNGF (proNGF123) plasmid in the retina using electroporation.
  73. ncbi Efficient genetic modification of murine dendritic cells by electroporation with mRNA
    Sonja Van Meirvenne
    Laboratory of Physiology Immunology of the Medical School of the Vrije Universiteit Brussel VUB, Brussels, Belgium
    Cancer Gene Ther 9:787-97. 2002
    ..Compared to a standard lipofection protocol and passive pulsing, electroporation was, in our hands, the most efficient method...
  74. pmc Effect of plasmid DNA vaccine design and in vivo electroporation on the resulting vaccine-specific immune responses in rhesus macaques
    Amara Luckay
    Wyeth Vaccines Research, 401 N Middletown Rd, Bldg 180 216 10, Pearl River, NY 10965, USA
    J Virol 81:5257-69. 2007
    ..and four-vector pDNA vaccine designs in nonhuman primates and to determine the extent to which in vivo electroporation (EP) could improve the resulting immune responses...
  75. ncbi Electrochemotherapy with cisplatin: the systemic antitumour effectiveness of cisplatin can be potentiated locally by the application of electric pulses in the treatment of malignant melanoma skin metastases
    G Sersa
    Institute of Oncology, Ljubljana, Slovenia
    Melanoma Res 10:381-5. 2000
    ..Therefore, electrochemotherapy may be used as an adjunct to systemic ongoing cisplatin treatment, predominantly in patients in whom antitumour effectiveness needs to be potentiated locally...
  76. ncbi High-efficiency transfection and survival rates of embryonic and adult mouse neural stem cells achieved by electroporation
    Bettina Bertram
    Department of Cell Morphology and Molecular Neurobiology, Ruhr University, Im Neuenheimer Feld 307, Bochum, Germany
    J Neurosci Methods 209:420-7. 2012
    ..This improved protocol for the transfection of sensitive mouse central nervous system derived cells will provide an important step forward for studies of gene function by overexpression or knock-down of genes in cultured NSCs...
  77. ncbi Codon-optimization of the hemagglutinin gene from the novel swine origin H1N1 influenza virus has differential effects on CD4(+) T-cell responses and immune effector mechanisms following DNA electroporation in mice
    M Tenbusch
    Department of Molecular and Medical Virology, Ruhr Universitat Bochum, Universitatsstr 150, 44801 Bochum, Germany
    Vaccine 28:3273-7. 2010
    DNA electroporation is a powerful vaccine strategy that could be rapidly adapted to address emerging viruses...
  78. ncbi Electroporation of immature and mature dendritic cells: implications for dendritic cell-based vaccines
    A Michiels
    Laboratory of Molecular and Cellular Therapy, Department of Physiology Immunology, Medical School of the Vrije Universiteit Brussel VUB, Brussels, Belgium
    Gene Ther 12:772-82. 2005
    Until now, studies utilizing mRNA electroporation as a tool for the delivery of tumor antigens to human monocyte-derived dendritic cells (DC) have focused on DC electroporated in an immature state...
  79. ncbi Cell counting tool parameters optimization approach for electroporation efficiency determination of attached cells in phase contrast images
    M Usaj
    Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia
    J Microsc 241:303-14. 2011
    ..For evaluation of electroporation efficiency of attached cells, we often perform manual counting of the cells which is needed to determine the ..
  80. pmc Examining the relationship between early axon growth and transcription factor expression in the developing cerebral cortex
    Tom Lickiss
    Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    J Anat 220:201-11. 2012
    ..b>Electroporation of an EYFP-expressing plasmid at embryonic day 13...
  81. ncbi Comparison of nonviral transfection and adeno-associated viral transduction on cardiomyocytes
    Srdjan Djurovic
    Department Institute of Medical Genetics, Ullevaal University Hospital, Kirkeveien 166, N 0407 Oslo, Norway
    Mol Biotechnol 28:21-32. 2004
    ..such as: FuGENE 6, LipofectAMINE 2000, LipofectAMINE PLUS, GenePORTER, Metafectene, and LipoGen; (2) electroporation and nucleofector techniques; and (3) an AAV2 vector harboring a lacZ reporter gene...
  82. ncbi Ca2+ imaging of identifiable neurons labeled by electroporation in insect brains
    Terufumi Fujiwara
    Graduate School of Information Science and Technology, The University of Tokyo, Tokyo, Japan
    Neuroreport 20:1061-5. 2009
    Identifiable neurons in the silkmoth brain were studied physiologically after loading of Ca indicator by local electroporation. Small groups of neurons with projections in confined regions of the antennal lobe were labeled, and Ca ..
  83. ncbi Optimisation of pulse parameters in vitro for in vivo electrochemotherapy
    Alenka Macek Lebar
    University of Ljubljana, Faculty of Electrical Engineering, Slovenia
    Anticancer Res 22:1731-6. 2002
    ..Our results demonstrated that electric pulse parameters for effective in vivo application can be determined from in vitro experiments considering application specifications...
  84. pmc Numerical optimization of gene electrotransfer into muscle tissue
    Anze Zupanic
    University of Ljubljana, Faculty of Electrical Engineering, Trzaska cesta 25, SI 1000 Ljubljana, Slovenia
    Biomed Eng Online 9:66. 2010
    b>Electroporation-based gene therapy and DNA vaccination are promising medical applications that depend on transfer of pDNA into target tissues with use of electric pulses...
  85. ncbi A novel method for the production of transgenic cloned pigs: electroporation-mediated gene transfer to non-cultured cells and subsequent selection with puromycin
    Satoshi Watanabe
    Department of Developmental Biology, Division of Insect and Animal, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305 0901, Japan
    Biol Reprod 72:309-15. 2005
    ..Results indicate that puromycin can be used for the selection of recombinant cells from noncultured cells, and moreover, may confer the production of genetically engineered newborns via nuclear transfer techniques in pigs...
  86. pmc Eya1-Six1 interaction is sufficient to induce hair cell fate in the cochlea by activating Atoh1 expression in cooperation with Sox2
    Mohi Ahmed
    Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA
    Dev Cell 22:377-90. 2012
    ..Our findings demonstrate that direct and cooperative interactions between the Sox2, Six1, and Eya1 proteins coordinate Atoh1 expression to specify hair cell fate...
  87. pmc Comparison of immune responses generated by optimized DNA vaccination against SIV antigens in mice and macaques
    Viraj Kulkarni
    Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Vaccine 29:6742-54. 2011
    ..DNA only immunization using intramuscular injection in the absence or presence of in vivo electroporation did not alter the phenotype of the induced T cell responses in mice...
  88. ncbi Simultaneous visualization of multiple neuronal properties with single-cell resolution in the living rodent brain
    Rie Ako
    Department of Molecular and Systems Neurobiology, Graduate School of Medicine, The University of Tokyo, Japan
    Mol Cell Neurosci 48:246-57. 2011
    ....
  89. pmc Comparison of plasmid vaccine immunization schedules using intradermal in vivo electroporation
    David Hallengärd
    Swedish Institute for Communicable Disease Control, Nobels Väg 18, 171 82 Solna, Stockholm, Sweden
    Clin Vaccine Immunol 18:1577-81. 2011
    In vivo electroporation (EP) has proven to significantly increase plasmid transfection efficiency and to augment immune responses after immunization with plasmids...
  90. pmc Diffusion-weighted MRI for verification of electroporation-based treatments
    Faisal Mahmood
    Center for Experimental Drug and Gene Electrotransfer, Department of Oncology, Copenhagen University Hospital, Herlev Ringvej 75, 2730 Herlev, Denmark
    J Membr Biol 240:131-8. 2011
    Clinical electroporation (EP) is a rapidly advancing treatment modality that uses electric pulses to introduce drugs or genes into, e.g., cancer cells...
  91. ncbi Equivalent pulse parameters for electroporation
    Gorazd Pucihar
    Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia
    IEEE Trans Biomed Eng 58:3279-88. 2011
    b>Electroporation-based applications require the use of specific pulse parameters for a successful outcome. When recommended values of pulse parameters cannot be set, similar outcomes can be obtained by using equivalent pulse parameters...
  92. ncbi Improvement of combined modality therapy with cisplatin and radiation using electroporation of tumors
    G Sersa
    Department of Tumor Biology, Institute of Oncology, Ljubljana, Slovenia
    Int J Radiat Oncol Biol Phys 46:1037-41. 2000
    To evaluate whether a local drug delivery method, i.e., electroporation of tumors, increases the radiosensitizing effect of cisplatin.
  93. pmc The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells
    Christelle Rosazza
    Department of Structural Biology and Biophysics, CNRS, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France
    Mol Ther 19:913-21. 2011
    ..The disruption of the microfilaments reduces the DNA accumulation at the plasma membrane and the gene expression. This is the first direct experimental evidence of the participation of the actin cytoskeleton in DNA electrotransfer...
  94. pmc Immunologic activity and safety of autologous HIV RNA-electroporated dendritic cells in HIV-1 infected patients receiving antiretroviral therapy
    Jean Pierre Routy
    Immunodeficiency Service and Division of Hematology, McGill University Health Centre, McGill University, Montreal, QC, Canada
    Clin Immunol 134:140-7. 2010
    ..No evidence of autoimmunity, changes in viral load, or significant changes in absolute CD4(+) and CD8(+) T cell counts were observed. This pilot study supports the further clinical investigation of AGS-004...
  95. ncbi Overexpression of Wld(S) or Nmnat2 in mauthner cells by single-cell electroporation delays axon degeneration in live zebrafish
    Yan Feng
    Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai, China
    J Neurosci Res 88:3319-27. 2010
    ..In this study, we labeled M-cells by single-cell electroporation with plasmids expressing DsRed2 or EGFP...
  96. ncbi Gene transfer of interleukin 10 to the murine cornea using electroporation
    Rui Zhou
    Division of Pulmonary and Critical Care Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Exp Biol Med (Maywood) 232:362-9. 2007
    ..We have used electroporation to deliver plasmids expressing interleukin 10 (IL-10) to the corneas of mice and evaluated the duration of ..
  97. pmc Vascular smooth muscle cells ablation with endovascular nonthermal irreversible electroporation
    Elad Maor
    Biophysics Graduate Group, Department of Mechanical Engineering, 6124 Etcheverry Hall, University of California, Berkeley, CA 94720, USA
    J Vasc Interv Radiol 21:1708-15. 2010
    To evaluate the effect of endovascular nonthermal irreversible electroporation (NTIRE) on blood vessels.
  98. pmc Gene transfer of tumor-reactive TCR confers both high avidity and tumor reactivity to nonreactive peripheral blood mononuclear cells and tumor-infiltrating lymphocytes
    Laura A Johnson
    Surgery Branch, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    J Immunol 177:6548-59. 2006
    ..highest avidity against MART-1 expressing tumors in vitro, both endogenously in the TIL clone, and after RNA electroporation into donor T cells...
  99. ncbi Whisker-related axonal patterns and plasticity of layer 2/3 neurons in the mouse barrel cortex
    Keisuke Sehara
    Department of Molecular and Systems Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    J Neurosci 30:3082-92. 2010
    ..We selectively visualized layer 2/3 neurons using in utero electroporation and examined the axonal trajectories of layer 2/3 neurons...
  100. ncbi Introduction of foreign DNA into the water flea, Daphnia magna, by electroporation
    Yasuhiko Kato
    Okazaki Institute for Integrative Bioscience, Japan
    Ecotoxicology 19:589-92. 2010
    ..Hence, we have developed a gene introduction technique based on electroporation. There are two critical points for the successful introduction of foreign DNA into D. magna...
  101. pmc Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggs
    Kristine J Kines
    Department of Microbiology, Immunology and Tropical Medicine, George Washington University Medical Center, Washington, DC, United States of America
    PLoS Negl Trop Dis 4:e593. 2010
    ....

Research Grants75

  1. Photodynamic Therapy of Localized Infections
    Michael R Hamblin; Fiscal Year: 2013
    ..We will explore te use of iontophoresis and electroporation singly and in combination to deliver antimicrobial PS (and iodide) into ex vivo pigskin and into hairless ..
  2. The Role of Astrocytes in Cortical Interneuron Development
    KAREN MULLER SMITH; Fiscal Year: 2013
    ..the translating ribosome affinity purification (TRAP) method, and target validation experiments using in vivo electroporation of silencing RNA (siRNA) constructs...
  3. Effects of environmental thyroid hormone disrupting compounds on CNS development
    CHRISTOPHER KIRK THOMPSON; Fiscal Year: 2013
    ..structural plasticity and brain circuit connectivity using targeted manipulation of gene expression via electroporation, time-lapse 2-photon imaging, neuronal reconstruction and structural analysis, electrophysiology and ..
  4. T-cell Therapy for B-lineage Acute Lymphoblastic Leukemia
    Laurence J N Cooper; Fiscal Year: 2013
    ..The Sleeping Beauty (SB) system has been combined with electroporation to introduce the CAR as well as co-express HSV-1 thymidine kinase (TK) for imaging by positron emission ..
  5. Role in Myopia Development of Retinal Pigment Epithelium - A New Therapeutic Targ
    Yan Zhang; Fiscal Year: 2013
    ..Techniques most commonly encountered in ocular gene therapy research, including electroporation and subretinal injection, will also be employed...
  6. Role of Coxiella burnetii group I introns in growth modulation
    Michael F Minnick; Fiscal Year: 2010
    ..The first goal will build on preliminary data showing that electroporation of Cbu.L1917 RNA impairs C. burnetii growth. Dose-dependency and the possibility that Cbu.L1917 and Cbu...
  7. Translational Immunology Research and Accelerated [vaccine] Development (TRIAD)
    ANNE SEARLS DEGROOT; Fiscal Year: 2013
    ..formulation, and adjuvanting by exploring a range of delivery options [Dendritic cells, DEC205, DNA, electroporation, mucosal delivery)...
  8. Novel Therapeutic Vaccines for Chronic HBV
    ROBERT GERALD WHALEN; Fiscal Year: 2013
    ..We propose to develop a therapeutic DNA vaccine product delivered by electroporation and expressing a mixture of HBsAg variants...
  9. Improve Wound Healing with HIF-CA5 DNA Vector and Electroporation
    John W Harmon; Fiscal Year: 2010
    ..provided by applicant): Therapy to Improve Wound Healing with DNA Expression Vector for HIF 11 and Electroporation Impaired wound healing is a tremendous problem for individuals with diabetes...
  10. Advancing a Lead Candidate Hantavirus Pulmonary Syndrome DNA Vaccine
    JAY WILLIAMS HOOPER; Fiscal Year: 2013
    ..Much of our preliminary data has involved delivering the DNA vaccines using either a gene gun, or muscle electroporation. Recently, we tested delivery of the SNV DNA vaccine using the PharmaJet needle-free jet injection device...
  11. Delivery of biologically active nucleic acids to epidermal cells
    ROGER L contact KASPAR; Fiscal Year: 2010
    ..and quantitative imaging devices to compare delivery methods that include, but need not be limited to: electroporation, sonoporation, iontophoresis, liquid jets, microneedles and topical formulations...
  12. Function and regulation of Lhx genes in craniofacial development
    Juhee Jeong; Fiscal Year: 2010
    ..I will verify their activity in chick neural crest electroporation system and transgenic mice...
  13. The Role of Alternative Splicing Factor Sfrs10 in Neural Development
    Rahul N Kanadia; Fiscal Year: 2010
    ..In addition, loss (RNAi) and gain of function will be performed by either in vivo DNA electroporation of a postnatal day 0 mouse retina or by in vitro electroporation of embryonic retinal explant cultures...
  14. Genetic Analysis of Mouse Nervous System Development
    Kathryn V Anderson; Fiscal Year: 2013
    ..In utero electroporation of GFP- tagged Cre will be used to conditionally delete Sas4 and Ift88 in the brain and then follow the fate ..
  15. Excitation-contraction Coupling in Normal and Dystrophic Mammalian Muscle
    Julio L Vergara; Fiscal Year: 2013
    ..and a fully functional ECC, we will take advantage of our ability to express DGC proteins by in vivo electroporation and use transgenic animal models with other genetic conditions altering the DGC (e.g. SSPN-Tg, and Utr-TET)...
  16. IL12 Gene Therapy For Enhancing Therapeutic Efficacy of Bleomycin Against Oral Tu
    Shulin Li; Fiscal Year: 2013
    ..Intratumoral injection of bleomycin via electroporation has proven very effective in treating highly malignant SCC tumors of the head and neck in a Phase II trial, ..
  17. MEMBRANE FUSION IN RETINAL ROD OUTER SEGMENTS
    KATHLEEN BOESZEBATTAGLIA; Fiscal Year: 2012
    ..In specific Aim #1a using in vivo electroporation to introduce genetically modified rds into the developing mouse retina we will determine how truncation and ..
  18. Axon Guidance in the Olfactory System
    DIEGO JAVIER RODRIGUEZ-GIL; Fiscal Year: 2013
    ..The role of Dvl molecules will be assessed by injecting a characterized silencing RNA (siRNA) by in utero electroporation, which I recently optimized to label OSNs in the OE...
  19. Role of Cyclins in Brain Construction and Interneuron Genesis
    KRISTIN GIAMANCO; Fiscal Year: 2013
    ..We will employ in utero electroporation, ex vivo slice electroporation, and time-lapse imaging to examine the role of cD2 in proliferation and ..
  20. Calcium Signaling in a Model of Temporal Lobe Epilepsy
    Karen S Wilcox; Fiscal Year: 2013
    ..We employ in utero electroporation to target a genetically encoded Ca2+ indicating protein (Lck- GCaMP3) to the rat HC so that we can use brain ..
  21. Insm1 in Development of Spiral and Vestibular Ganglia
    Sarah M Lorenzen; Fiscal Year: 2013
    ..expression on delamination, proliferation, and neuron production by driving over expression using in utero electroporation. Aim 3: To establish if Insm1 is necessary for differentiation of SAG neurons...
  22. In utero protein transduction to interrogate inner ear sensory patch formation
    JOHN VINCENT BRIGANDE; Fiscal Year: 2013
    ..that permit gain-of-function studies in the developing mouse inner ear that rely on viral vectors and in vivo electroporation. In the present proposal, we seek to define a rapid, cost effective, and technically simplified experimental ..
  23. Functional analysis of AMD-associated HTRA1 promoter polymorphism
    Noriko Esumi; Fiscal Year: 2010
    ..Our analyses to explore this hypothesis using in vivo electroporation in mouse RPE showed that the HTRA1 promoter with risk-associated A had a significantly lower activity than ..
  24. Cytoplasmic trafficking of non-viral gene therapy vectors
    David A Dean; Fiscal Year: 2012
    ..Finally, we have developed an electroporation method for high-level, safe, non-viral gene transfer to the lung and have used this approach to treat lung ..
  25. DNA Nuclear Import Sequences for Cell-Specific Gene and Drug Delivery
    David A Dean; Fiscal Year: 2013
    ..Finally, validated hits from the previous aims will be delivered by electroporation to individual organs in vivo to test for cell- and tissue-specific gene delivery...
  26. Nanocapsule-mediated topical delivery of VLP-generating Dengue DNA vaccine
    Gretchen M Unger; Fiscal Year: 2013
    ..naked DNA delivered via electroporation and via intramuscular injection...
  27. Targeting mTOR and CD40 Pathways for Adjuvanting HIV Vaccines
    Rama Rao Amara; Fiscal Year: 2013
    ..In addition, we will test whether delivering DNA by electroporation enhances the immunogenicity of the adjuvanted and non-adjuvanted DN/VMVA vaccines. Recent studies from Dr...
  28. Targeting Airway Smooth Muscle for Asthma Gene Therapy
    David A Dean; Fiscal Year: 2013
    ..Using in vivo electroporation, we plan to test the efficacy of our smooth muscle-specific nuclear import sequence for gene transfer in ..
  29. NEUROGENETIC PROCESSES IN THE FETAL BRAIN
    Pasko Rakic; Fiscal Year: 2013
    ..In Specific Aim #1 we use the most advanced methods, including the triple KO technology, electroporation and multi-photon microscopy both in vivo and in vitro, to continue our analysis of the molecular mechanisms ..
  30. Mechanisms of MCP-1 in lung contusion with and without gastric aspiration
    Krishnan Raghavendran; Fiscal Year: 2013
    ..Aim 3 examines the effects of delivering exogenous MCP-1 to the lungs via direct instillation and by electroporation-facilitated gene delivery of DNA plasmids containing MCP-1 to MCP-1 (-/-) and wild type mice following lung ..
  31. Investigating the role of ribeye in retinal ribbon function
    DAVID PAUL ZENISEK; Fiscal Year: 2013
    ..having effects on b-wave of the ERG in zebra fish will be introduced into mouse rod bipolar cells by in vivo electroporation and then used for paired whole-cell recordings...
  32. Regulation of Rhodopsin Expression
    Donald J Zack; Fiscal Year: 2012
    ..candidate molecules for regulating the various cone opsins;2) further develop and utilize in vitro retinal electroporation technology so as to assess the biological activity of rod and cone opsin regulatory elements in a more ..
  33. Sleeping Beauty-mediated Gene Therapy of X-linked SCID
    Kendra A Hyland; Fiscal Year: 2010
    ..here we propose that X-SCID may be treatable without the use of a virus by combining the power of electroporation for introduction of DNA into cells, along with use of DGI's Sleeping Beauty transposon system to achieve ..
  34. MicroRNA and Muscle Wasting in Uremia
    XIAONAN H WANG; Fiscal Year: 2013
    ..We will use in vivo gene transfer of miR29 by lentivirus and electroporation to test our theory...
  35. MOLECULAR BASIS OF TOOTH ERUPTION
    Shaomian Yao; Fiscal Year: 2012
    ..Each gene that is upregulated more in the basal portion will then be silenced in vivo using electroporation and RNAi technology specific for the target mRNA of each gene to determine the effect of each gene on ..
  36. High Fat Feeding Promotes Malfunction of the Nodose Ganglia
    Chung Owyang; Fiscal Year: 2013
    ..the reversibility of electrophysiological abnormalities following silencing TRESK channel expression through electroporation of the NG with TRESK siRNA...
  37. Regulation of Ptf1a in the Developing Nervous System
    David M Meredith; Fiscal Year: 2010
    ..Requirement of both halves of the PTF1 sites will be tested by mutation and reporter gene assays by in ovo electroporation in chick...
  38. Regulation of axonal outgrowth in the developing spinal cord
    Samantha J Butler; Fiscal Year: 2012
    ..in these studies, combining biochemical and tissue grafting assays with mouse genetics and chick in ovo electroporation, in the following aims: Aim 1: Determine how BMP signaling regulates Limk1/cofilin to control the rate of C ..
  39. Assessment of the Immunogenicity and Protective Efficacy of a Multiagent DNA Vacc
    Connie Schmaljohn; Fiscal Year: 2010
    ..While DNA vaccines have lacked sufficient potency in humans, the development of in vivo electroporation delivery systems has the potential to address this shortcoming...
  40. IL-15 receptor-alpha and IL-15 action in aging skeletal muscle and adipose tissue
    LeBris S Quinn; Fiscal Year: 2013
    ..In vivo, the innovative and translational technique of plasmid electroporation into skeletal muscle (EPM) will be used to induce systemic expression of IL-15, sIL-15R1 variants, and a ..
  41. Reactive Oxygen Species in Coronary Collateral Growth
    William M Chilian; Fiscal Year: 2013
    ..Cells will be transfected with a plasmid (using in vivo electroporation) or transduced with an adenovirus expressing an iNOS mutant (E371A) that does not bind arginine, and ..
  42. Development and Plasticity of a Retinotectal System
    Mu ming Poo; Fiscal Year: 2012
    ..2) expression of wild-type or mutated forms of Ephrin-B1 or EphB2 in a few RGCs or tectal cells by electroporation, and (3) global tetracycline-induced transgenic expression of these proteins in either all presynaptic RGCs ..
  43. eukaryotic expression vectors resistant to transgene silencing
    James Williams; Fiscal Year: 2012
    ..Previous innovations such as electroporation (EP) increased transgene expression through more effective gene delivery...
  44. RGK proteins and aging muscle
    ROGER ALAN BANNISTER; Fiscal Year: 2012
    ..To do so, cDNA plasmids encoding RGK proteins will be delivered to mouse muscle via in vivo electroporation. The validity of this new model system will be assessed with patch-clamp electrophysiology and Ca imaging...
  45. Multichannel Electrosonic Actuation Microarray for Cell-Based Screening
    JOHN MARK MEACHAM; Fiscal Year: 2010
    ..e., concurrent sono/mechanoporation and electroporation). The device format is compatible with existing large-scale cell-handling equipment...
  46. Combining AD epitope vaccine with innate immunity
    DAVID HASTINGS CRIBBS; Fiscal Year: 2013
    ..to scale up DNA immunization;3) to compare intramuscular versus intradermal DNA immunization using electroporation system that is currently being used in human clinical trials for other vaccine applications...
  47. Method Development for a Next Generation Miniaturized Genomic Screening Platform
    Peter Kahn; Fiscal Year: 2012
    ..Our collaborator on this grant, Dr. Saez, has co-invented a novel HTS gene function platform, called, 'electroporation-ready- microwell-arrays', that will allow whole human genome screens on a single plate that is ready for ..
  48. A novel genetic and electrophysiological approach to study hippocampal neurons
    Tarik F Haydar; Fiscal Year: 2009
    ..Using electroporation to transfect the hippocampal stem cells in utero, we will introduce fluorescent protein markers as well as ..
  49. Preclinical Assessment of a Multi-Head Electroporation Device for Delivery of Bio
    Connie Schmaljohn; Fiscal Year: 2013
    ..We showed that a codon optimized DNA vaccine for LASV delivered by intradermal electroporation (ID-EP) is completely protective against LASV challenge in both guinea pig and nonhuman primate disease ..
  50. Role of genome organizer Satb1 in Shh-dependent proliferation and tumorigenesis
    FRANCES YUN CHENG; Fiscal Year: 2013
    ..expression of Satb1 using a gain and loss-of-function approach in cultured primary CGNPs and using in vivo electroporation. In our second aim, we seek to define the role of Satb1 during hedgehog-mediated oncogenic transformation...
  51. Robo-Slit Signaling in Avian Cornea Innervation
    Tyler Schwend; Fiscal Year: 2013
    ..by inhibiting Robo-Slit signaling and studying the developmental consequences using organ cultures and in ovo electroporation so that the pathway may be studied in living organs and developing chickens...
  52. Design of Degron-based Substrates Capable of E3 Ligase-mediated Ubiquitination
    ADAM THOMAS MELVIN; Fiscal Year: 2013
    ..A rigorous study will be conducted to determine the ideal method, ranging from electroporation to myristoylation, to insert the substrate into one of the aforementioned cell lines...
  53. Role of actin cytoskeleton regulators in craniofacial development and disease
    Shuyi Nie; Fiscal Year: 2013
    ..both model systems: simple gene and tissue manipulation in frog, and high-resolution imaging and late stage electroporation in chick...
  54. Endothelin and EGFR Activation in Pulmonary Hypertension
    Olga Rafikova; Fiscal Year: 2012
    ..Finally, we will determine if using gene delivery of the ETB receptor to the rat lung using electroporation will increase EGFR protein nitration, reduce VSMC proliferation, and alleviate the changes associated with ..
  55. Enhancing DNA vaccines using modified Bacterial Toxin A1 Subunits as adjuvants
    Timothy R Fouts; Fiscal Year: 2012
    ..nearly 2-fold higher than those induced by the "gold-standard" adjuvant IL-12 plasmid DNA (pDNA) or in vivo electroporation. In this Phase II application, we propose screen additional mutants and select a lead A1 subunit adjuvant to ..
  56. Investigation of Endocytosis Involved in Electrotransfection
    Fan Yuan; Fiscal Year: 2013
    ..g., pulse shape and field strength) for improving transient pore creation in cell membrane (i.e., electroporation) or plasmid DNA (pDNA) transport through the pores via electrophoresis...
  57. Dorsal midline patterning in the vertebrate CNS
    KATHLEEN JOYCE MILLEN; Fiscal Year: 2013
    ..development through analysis of extant mouse mutants and manipulation of gene expression using in utero mouse electroporation. Our preliminary data demonstrate that Lmx1a also regulates neurogenesis in the telencephalic cortical hem, ..
  58. Cytokine Regulation of Muscle Protein Synthesis During Infection
    Charles H Lang; Fiscal Year: 2013
    ..It is noteworthy that the proposed in vivo electroporation of shRNA or over-expression plasmids specifically to skeletal muscle permits loss- and gain-of-function ..
  59. Functioning of Nodose Ganglia in Diabetes
    Chung Owyang; Fiscal Year: 2013
    ..of the electrophysiological and GI abnormalities following silencing TRESK channel expression in vivo through electroporation of the NG with TRESK siRNA...
  60. Inpp5f regulates CNS axon regeneration through phosphoinositide metabolism
    Yixiao Zou; Fiscal Year: 2013
    ..In addition, the proposed experiments will demonstrate the utility of a novel technique, in vivo retinal electroporation in adult mice, in discovering and dissecting genetic regulation of CNS axon regeneration...
  61. Role of Staufen2 in asymmetric division of mouse cortical progenitor cells
    GRETCHEN K KUSEK; Fiscal Year: 2010
    ..In utero electroporation will be used to deliver Stau2 shRNA, overexpression, or control constructs into the developing embryonic ..
  62. Development of low cost and broadly protective human papillomavirus vaccines
    RICHARD BRUCE RODEN; Fiscal Year: 2010
    ..vectors or HPV L1-polymeric L2 expression vectors using vaginal challenge in mice vaccinated by in vivo electroporation. The potential of these approaches will be directly compared in an intravaginal HPV challenge model to assess ..
  63. Electroporation and transcutaneous extraction of drugs
    SATHYANARAYANA MURTHY; Fiscal Year: 2006
    ..We propose to develop a noninvasive, simple and rapid "Electroporation and Transcutaneous extraction (ETE)" to sample the drugs from the dermal ECF...
  64. ELECTRICALLY MODULATED SKIN DELIVERY OF BETA-BLOCKERS
    Ajay Banga; Fiscal Year: 2000
    ..g., current density) or in combination with electroporation (e.g...
  65. Potentiating an anthrax DNA vaccine with electroporation
    Brian Livingston; Fiscal Year: 2005
    ..The method of application of a DNA vaccine is critical for efficacy. Vaccine will be delivered by electroporation because this method provides consistent, high level, scalable immunogen expression, which correlates with ..
  66. VIRULENCE IN COXIELLA BURNETTII
    HERBERT THOMPSON; Fiscal Year: 1999
    ..In aim 1, organisms will be transformed to ampicillin resistance using a mixture of plasmids en masse via electroporation. These plasmids carry the C. burnetii autonomous replication sequence (ars) and a ColE1 origin...
  67. Identification of Factors Influencing Climbing Fiber Neuron Fate in the Dorsal Hi
    REBECCA LANDSBERG; Fiscal Year: 2009
    ..In Aim 1, an in vitro hindbrain electroporation technique will be employed to identify molecular factors responsible specifying ION fate...
  68. ASPARTATE TRANSMISSION
    J Victor Nadler; Fiscal Year: 2005
    ..A method will be developed to introduce polypeptides and proteins into the synaptosomes by an electroporation technique that does not disrupt glutamate/aspartate release significantly...
  69. Development of a Malaria DNA Vaccine with Enchanced Immunogenicity
    Richard Markham; Fiscal Year: 2009
    ..vivo the responses elicited in BALB/c mice by a candidate multi-epitope DNA vaccine administered by in vivo electroporation that targets the pre-erythrocytic stages of P. yoelii...
  70. ANTIBODIES TO DISSECT SENSORY TRANSDUCTION IN PARAMECIUM
    David Nelson; Fiscal Year: 1990
    ..Methods will be developed for adding antibodies to living cells by microinjection, electroporation or reversible permeabilization...
  71. Transposon-mediated Gene Therapy for Fanconi Anemia
    PERRY HACKETT; Fiscal Year: 2004
    ..MaxCyte will then use its electroporation technology for high efficiency loading of the transposon DNA into several different hematopoietic cell lines (..
  72. TUMOR CELL KILLING BY EXPRESSION OF A TOXIN GENE
    IAN MAXWELL; Fiscal Year: 1992
    ..stringent control over the expression of a toxin gene when introduced into mammalian cells in culture, using electroporation (specific aims (1) to (3)), or in transgenic mice as an in vivo model (aim #(4))...
  73. Feedback and Hormonal Regulation of Hepatic HMG-CoA Reductase
    GENE CHARLES NESS; Fiscal Year: 2010
    ..regions ligated to luciferase directly into livers of diabetic or insulin-treated diabetic rats by in vivo electroporation or by hydrodynamic tail vein injection...
  74. Development of Avian Extraocular Muscles and Nerves
    Drew Noden; Fiscal Year: 2007
    ..This will be done using electroporation and infection with RCAS retroviral constructs...
  75. GENETIC SYSTEMS FOR FUSOBACTERIUM NUCLEATUM
    Susan Kinder Haake; Fiscal Year: 2002
    ..nucleatum. In Specific Aim 1 a gene transfer system for F. nucleatum will be developed using electroporation with pFN1-based plasmids that encode a tetracycline selectable marker...