colony forming units assay

Summary

Summary: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.

Top Publications

  1. ncbi The long-term repopulating subset of hematopoietic stem cells is deterministic and isolatable by phenotype
    S J Morrison
    Department of Pathology, Stanford University School of Medicine, California 94305, USA
    Immunity 1:661-73. 1994
  2. ncbi Chemokine SDF-1 enhances circulating CD34(+) cell proliferation in synergy with cytokines: possible role in progenitor survival
    J J Lataillade
    Laboratoire d Immunologie Cellulaire, centre de transfusion sanguine des armées Jean Julliard, Clamart Cedex, France
    Blood 95:756-68. 2000
  3. ncbi Haploinsufficiency of CBFA2 causes familial thrombocytopenia with propensity to develop acute myelogenous leukaemia
    W J Song
    Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Nat Genet 23:166-75. 1999
  4. ncbi Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4
    A Peled
    Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Science 283:845-8. 1999
  5. pmc PU.1 regulates both cytokine-dependent proliferation and differentiation of granulocyte/macrophage progenitors
    R P DeKoter
    Department of Molecular Genetics and Cell Biology, The University of Chicago, IL 60637, USA
    EMBO J 17:4456-68. 1998
  6. ncbi Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization
    Lindolfo da Silva Meirelles
    Laboratorio de Imunogenetica, Departamento de Genetica, Universidade Federal do Rio Grande do Sul, Av Bento Goncalves, Porto Alegre RS, Brasil
    Br J Haematol 123:702-11. 2003
  7. ncbi Hematopoietic stem cell quiescence maintained by p21cip1/waf1
    T Cheng
    Experimental Hematology, AIDS Research Center, Massachusetts General Hospital Cancer Center, Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Science 287:1804-8. 2000
  8. ncbi Adult mouse hematopoietic stem cells: purification and single-cell assays
    Hideo Ema
    Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Protoc 1:2979-87. 2006
  9. ncbi Stat5 is essential for the myelo- and lymphoproliferative disease induced by TEL/JAK2
    J Schwaller
    Division of Hematology and Oncology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 6:693-704. 2000
  10. ncbi Circulating endothelial progenitor cells, vascular function, and cardiovascular risk
    Jonathan M Hill
    Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1622, USA
    N Engl J Med 348:593-600. 2003

Research Grants

Detail Information

Publications210 found, 100 shown here

  1. ncbi The long-term repopulating subset of hematopoietic stem cells is deterministic and isolatable by phenotype
    S J Morrison
    Department of Pathology, Stanford University School of Medicine, California 94305, USA
    Immunity 1:661-73. 1994
    ..The ability to predict the longevity of reconstitution based on lineage marker expression indicates that reconstitution potential is deterministic, not stochastic...
  2. ncbi Chemokine SDF-1 enhances circulating CD34(+) cell proliferation in synergy with cytokines: possible role in progenitor survival
    J J Lataillade
    Laboratoire d Immunologie Cellulaire, centre de transfusion sanguine des armées Jean Julliard, Clamart Cedex, France
    Blood 95:756-68. 2000
    ..Furthermore, both adhesion-induced CXCR-4 overexpression and SDF-1 stimulating activity may be of clinical relevance for improving cell therapy settings in stem cell transplantation...
  3. ncbi Haploinsufficiency of CBFA2 causes familial thrombocytopenia with propensity to develop acute myelogenous leukaemia
    W J Song
    Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Nat Genet 23:166-75. 1999
    ..Our findings support a model for FPD/AML in which haploinsufficiency of CBFA2 causes an autosomal dominant congenital platelet defect and predisposes to the acquisition of additional mutations that cause leukaemia...
  4. ncbi Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4
    A Peled
    Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Science 283:845-8. 1999
    ..Thus, up-regulation of CXCR4 expression may be useful for improving engraftment of repopulating stem cells in clinical transplantation...
  5. pmc PU.1 regulates both cytokine-dependent proliferation and differentiation of granulocyte/macrophage progenitors
    R P DeKoter
    Department of Molecular Genetics and Cell Biology, The University of Chicago, IL 60637, USA
    EMBO J 17:4456-68. 1998
    ..1 into mutant progenitors restores responsiveness to myeloid-specific cytokines and development of mature granulocytes and macrophages. Thus PU.1 controls myelopoiesis by regulating both proliferation and differentiation pathways...
  6. ncbi Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization
    Lindolfo da Silva Meirelles
    Laboratorio de Imunogenetica, Departamento de Genetica, Universidade Federal do Rio Grande do Sul, Av Bento Goncalves, Porto Alegre RS, Brasil
    Br J Haematol 123:702-11. 2003
    ....
  7. ncbi Hematopoietic stem cell quiescence maintained by p21cip1/waf1
    T Cheng
    Experimental Hematology, AIDS Research Center, Massachusetts General Hospital Cancer Center, Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Science 287:1804-8. 2000
    ..Under conditions of stress, restricted cell cycling is crucial to prevent premature stem cell depletion and hematopoietic death...
  8. ncbi Adult mouse hematopoietic stem cells: purification and single-cell assays
    Hideo Ema
    Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Protoc 1:2979-87. 2006
    ..Initialization of single-cell culture requires approximately 1 h. Single-cell transplantation requires approximately 6 h. Single-cell immunostaining requires approximately 2 d...
  9. ncbi Stat5 is essential for the myelo- and lymphoproliferative disease induced by TEL/JAK2
    J Schwaller
    Division of Hematology and Oncology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 6:693-704. 2000
    ..These data define a critical role for Stat5a/b and mOSM in the pathogenesis of TEL/JAK2 disease...
  10. ncbi Circulating endothelial progenitor cells, vascular function, and cardiovascular risk
    Jonathan M Hill
    Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1622, USA
    N Engl J Med 348:593-600. 2003
    ....
  11. ncbi The Barrett's antigen anterior gradient-2 silences the p53 transcriptional response to DNA damage
    Elizabeth Pohler
    Cancer Research UK Laboratories, Department of Molecular and Cellular Pathology, University of Dundee, Dundee DD1 9SY, United Kingdom
    Mol Cell Proteomics 3:534-47. 2004
    ..These data identify anterior gradient-2 as a novel survival factor whose study may shed light on cellular pathways that attenuate the tumor suppressor p53...
  12. ncbi Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders
    E Joanna Baxter
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
    Lancet 365:1054-61. 2005
    ..We investigated the role of the cytoplasmic tyrosine kinase JAK2 in patients with a myeloproliferative disorder...
  13. ncbi Number and function of endothelial progenitor cells as a marker of severity for diabetic vasculopathy
    Gian Paolo Fadini
    Department of Clinical and Experimental Medicine, Division of Metabolic Diseases, University of Padova, School of Medicine, Italy
    Arterioscler Thromb Vasc Biol 26:2140-6. 2006
    ..This study aimed to establish whether number and function of EPCs correlate with PAD severity in type 2 diabetic patients...
  14. pmc Nuclear factor-kappaB enhances ErbB2-induced mammary tumorigenesis and neoangiogenesis in vivo
    Manran Liu
    Department of Cancer Biology, Kimmel Cancer Center, Jefferson Stem Cell and Regenerative Medicine Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Am J Pathol 174:1910-20. 2009
    ..Thus, mammary epithelial cell NF-kappaB activity enhances ErbB2-mediated mammary tumorigenesis in vivo by promoting both growth and survival signaling via the promotion of tumor vasculogenesis...
  15. ncbi Global gene expression profile of human cord blood-derived CD133+ cells
    Taina Jaatinen
    Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland
    Stem Cells 24:631-41. 2006
    ..This study provides a gene expression profile for human CD133+ cells. It presents a set of genes that may be used to unravel the properties of the CD133+ cell population, assumed to be highly enriched in HSCs...
  16. ncbi Comparative analysis of methods for assessment of circulating endothelial progenitor cells
    Jacob George
    The Department of Cardiology, Tel Aviv University, Israel
    Tissue Eng 12:331-5. 2006
    ..These findings may suggest that CD34/KDR is more appropriate for the definition of circulating EPC, whereas CFU numbers are more likely to reflect their ability to proliferate...
  17. ncbi MOZ-TIF2, but not BCR-ABL, confers properties of leukemic stem cells to committed murine hematopoietic progenitors
    Brian J P Huntly
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Cancer Cell 6:587-96. 2004
    ..These data demonstrate that some, but not all, leukemia oncogenes can confer properties of leukemic stem cells to hematopoietic progenitors destined to undergo apoptotic cell death...
  18. ncbi The granulocyte colony-stimulating factor receptor is required for the mobilization of murine hematopoietic progenitors into peripheral blood by cyclophosphamide or interleukin-8 but not flt-3 ligand
    F Liu
    Department of Medicine, Washington University Medical School, St Louis, MO 63110 1093, USA
    Blood 90:2522-8. 1997
    ..These results show that the G-CSFR is required for mobilization in response to cyclophosphamide or IL-8 but not flt-3 ligand and suggest that the G-CSFR may play an important and previously unexpected role in HPC migration...
  19. ncbi Haploinsufficiency of AML1 affects the temporal and spatial generation of hematopoietic stem cells in the mouse embryo
    Z Cai
    Department of Pathology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Immunity 13:423-31. 2000
    ..Moreover, haploinsufficiency results in a dramatic change in the temporal and spatial distribution of HSCs, leading to their early appearance in the normal position in the aorta-gonad-mesonephros region and also in the yolk sac...
  20. pmc Foxm1b transcription factor is essential for development of hepatocellular carcinomas and is negatively regulated by the p19ARF tumor suppressor
    Vladimir V Kalinichenko
    University of Illinois at Chicago, College of Medicine, Department of Biochemistry and Molecular Genetics, Chicago, Illinois 60607, USA
    Genes Dev 18:830-50. 2004
    ..Our studies demonstrate that the Foxm1b transcription factor is required for proliferative expansion during tumor progression and constitutes a potential new target for therapy of human HCC tumors...
  21. ncbi Survivin regulates hematopoietic progenitor cell proliferation through p21WAF1/Cip1-dependent and -independent pathways
    Seiji Fukuda
    Department of Microbiology and Immunology, Walther Oncology Center, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Blood 103:120-7. 2004
    ....
  22. ncbi Hematopoietic progenitor/stem cells immortalized by Lhx2 generate functional hematopoietic cells in vivo
    Perpétua Pinto do Ó
    Department of Molecular Biology, Umea University, Sweden
    Blood 99:3939-46. 2002
    ..Thus, Lhx2 immortalizes multipotent hematopoietic progenitor/stem cells that can generate functional progeny following transplantation into lethally irradiated hosts and can long-term repopulate stem cell-deficient hosts...
  23. ncbi Myeloid development is selectively disrupted in PU.1 null mice
    K L Anderson
    The Burnham Institute, La Jolla, CA, USA
    Blood 91:3702-10. 1998
    ..1 gene disruption affects a number of developmentally regulated hematopoietic processes that can, at least in part, explain the changes in myeloid development and reduction in myeloid and neutrophil expansion observed in PU.1 null mice...
  24. pmc Dominant myelopoietic effector functions mediated by chemokine receptor CCR1
    H E Broxmeyer
    Department of Microbiology Immunology, The Department of Medicine, and the Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    J Exp Med 189:1987-92. 1999
    ..CCR1 is not a dominant receptor for MIP-1alpha suppression of MPC proliferation, but it does negatively impact G-CSF-induced MPC mobilization...
  25. ncbi Lhx2 expression in hematopoietic progenitor/stem cells in vivo causes a chronic myeloproliferative disorder and altered globin expression
    Karin Richter
    Umeå Center for Molecular Medicine, Umea University, 901 87 Umea, Sweden
    Haematologica 88:1336-47. 2003
    ..We have previously shown that Lhx2 expression in mouse HSC generates cytokine-dependent stem cell-like cell lines that can produce long-term repopulation in stem cell-deficient mice...
  26. ncbi Identification and characterization of an inhibitor of haemopoietic stem cell proliferation
    G J Graham
    Beatson Institute for Cancer Research, Glasgow, UK
    Nature 344:442-4. 1990
    ....
  27. ncbi Chemokine regulation of hematopoiesis and the involvement of pertussis toxin-sensitive G alpha i proteins
    H E Broxmeyer
    Departments of Microbiology and Immunology, and Medicine Hematology Oncology, The Walther Oncology Center, Indiana University School of Medicine, and the Walther Cancer Institute, Indianapolis, Indiana 46202, USA
    Ann N Y Acad Sci 938:117-27; discussion 127-8. 2001
    ..These results suggest that not all chemokine-mediated effects on MPCs are necessarily mediated through pertussis toxin-sensitive G alpha i proteins...
  28. ncbi Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow
    Stan Gronthos
    Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia
    J Cell Sci 116:1827-35. 2003
    ..Our novel selection protocol provides a means to generate purified populations of BMSSCs for use in a range of different tissue engineering and gene therapy strategies...
  29. ncbi Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure
    Christine K Kissel
    Department of Cardiology, Johann Wolfgang Goethe University Frankfurt, Frankfurt, Germany
    J Am Coll Cardiol 49:2341-9. 2007
    ....
  30. ncbi Mesenchymal stem cells derived from CD133-positive cells in mobilized peripheral blood and cord blood: proliferation, Oct4 expression, and plasticity
    Tatiana Tondreau
    Jules Bordet Institute, 121 Bd de Waterloo, 1000 Brussels, Belgium
    Stem Cells 23:1105-12. 2005
    ..The selection of CD133-positive cells enabled us to obtain a homogeneous population of MSCs from UCB and MPB. These sources may have a major clinical importance thanks to their easy accessibility...
  31. ncbi Regulation of macrophage and neutrophil cell fates by the PU.1:C/EBPalpha ratio and granulocyte colony-stimulating factor
    Richard Dahl
    Howard Hughes Medical Institute, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nat Immunol 4:1029-36. 2003
    ..1. Collectively, these results indicate that cytokines can promote cell fate decisions by altering the relative concentrations of lineage-determining transcriptional regulators...
  32. ncbi A common precursor for hematopoietic and endothelial cells
    K Choi
    Department of Pathology, University of Maryland at Baltimore, Baltimore, MD 21201, USA
    Development 125:725-32. 1998
    ..These findings provide strong evidence that the blast colony-forming cell represents the long-hypothesized hemangioblast, the common precursor of the hematopoietic and endothelial lineages...
  33. ncbi Hereditary anemias of the mouse: a review for geneticists
    E S Russell
    Adv Genet 20:357-459. 1979
  34. pmc Expression and function of c-kit in hemopoietic progenitor cells
    M Ogawa
    Department of Pathology, Kumamoto University Medical School, Japan
    J Exp Med 174:63-71. 1991
    ..These results provide direct evidence that c-kit is an essential molecule for constitutive intramarrow hemopoiesis, especially for the self-renewal of hemopoietic progenitor cells at various stages of differentiation...
  35. ncbi Bone marrow mobilization with granulocyte macrophage colony-stimulating factor improves endothelial dysfunction and exercise capacity in patients with peripheral arterial disease
    Veerappan Subramaniyam
    Division of Cardiology, Emory University School of Medicine, 1364 Clifton Rd, Atlanta, GA 30322, USA
    Am Heart J 158:53-60.e1. 2009
    ....
  36. ncbi Hyaluronic acid facilitates the recovery of hematopoiesis following 5-fluorouracil administration
    Vera Y Matrosova
    National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland, USA
    Stem Cells 22:544-55. 2004
    ..Overall, our results suggest that HA is required for regulation of the hematopoiesis-supportive function of bone marrow accessory cells and, therefore, participates in hematopoietic niche assembly...
  37. ncbi Maintaining the self-renewal and differentiation potential of human CD34+ hematopoietic cells using a single genetic element
    James C Mulloy
    Cincinnati Children s Hospital Medical Center, 3333 Burnet Ave, Mail Location 7013, Cincinnati, OH 45229, USA
    Blood 102:4369-76. 2003
    ....
  38. pmc Endothelial selectins and vascular cell adhesion molecule-1 promote hematopoietic progenitor homing to bone marrow
    P S Frenette
    The Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 95:14423-8. 1998
    ..Optimal recruitment of HPC after lethal doses of irradiation requires the combined action of both selectins and VCAM-1 expressed on endothelium of the BM...
  39. pmc Hematopoietic stem cell deficiencies in mice lacking c-Mpl, the receptor for thrombopoietin
    S Kimura
    The Walter and Eliza Hall Institute for Medical Research and The Cooperative Research Centre for Cellular Growth Factors PO Royal Melbourne Hospital, Victoria 3050, Australia
    Proc Natl Acad Sci U S A 95:1195-200. 1998
    ..These data imply that TPO, signaling through c-Mpl, plays a vital physiological role in the regulation of hematopoietic stem cell production and function...
  40. ncbi Aging, exercise, and endothelial progenitor cell clonogenic and migratory capacity in men
    Greta L Hoetzer
    Integrative Vascular Biology Laboratory, Dept of Integrative Physiology, University of Colorado, Boulder, CO 80309, USA
    J Appl Physiol 102:847-52. 2007
    ..Regular aerobic-endurance exercise, however, is an effective lifestyle intervention strategy for improving EPC clonogenic and migratory capacity in middle-aged and older healthy men...
  41. ncbi Endothelial progenitor cells and endothelial dysfunction
    J M Hill
    The Laboratory of Molecular Biology Cardiology Branch of the National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Vox Sang 87:31-7. 2004
  42. ncbi Increased apoptosis of bone marrow CD34(+) cells and impaired function of bone marrow stromal cells in patients with systemic lupus erythematosus
    H A Papadaki
    Department of Haematology, University of Crete School of Medicine, Crete, Greece
    Br J Haematol 115:167-74. 2001
    ..We concluded that defective haemopoiesis in SLE patients is probably caused, at least in part, to the presence of autoreactive lymphocytes in BM...
  43. ncbi Two distinct stem cell lineages in murine bone marrow
    Yoko Koide
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Stem Cells 25:1213-21. 2007
    ..The results may reinforce the urgent need for a more comprehensive view of the mesenchymal stem cell identity and characteristics. Disclosure of potential conflicts of interest is found at the end of this article...
  44. ncbi Cellular ITAM-containing proteins are oncoproteins in nonhematopoietic cells
    S M Grande
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Oncogene 25:2748-57. 2006
    ..Despite lacking intrinsic enzymatic activity, ITAM-containing proteins can function as potent oncoproteins by scaffolding downstream mediators...
  45. ncbi Endothelial colony forming capacity is related to C-reactive protein levels in healthy subjects
    Michele M Ciulla
    Istituto di Medicina Cardiovascolare, Centro di Fisiologia Clinica e Ipertensione, Universita di Milano, Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, IRCCS, Italy
    Curr Neurovasc Res 3:99-106. 2006
    ..The EPC clonogenic potential seems to be related to hs-CRP and VEGF levels even in healthy population supporting the concept that these mediators are involved in physiological ECs function...
  46. ncbi Murine embryonic stem cells secrete cytokines/growth modulators that enhance cell survival/anti-apoptosis and stimulate colony formation of murine hematopoietic progenitor cells
    Ying Guo
    Department of Microbiology Immunology, Walther Oncology Center, Indiana University School of Medicine, 950 West Walnut Street, R2 302, Indianapolis, Indiana 46202, USA
    Stem Cells 24:850-6. 2006
    ....
  47. ncbi Unresolved questions, changing definitions, and novel paradigms for defining endothelial progenitor cells
    David A Ingram
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 1044 W Walnut St, R4 402e, Indianapolis, IN 46202, USA
    Blood 106:1525-31. 2005
    ..The potential implications of this view in relation to current EPC definitions are discussed...
  48. ncbi SCID-repopulating cell activity of human cord blood-derived CD34- cells assured by intra-bone marrow injection
    Jianfeng Wang
    Department of Hygiene, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyoku, Kyoto, Japan
    Blood 101:2924-31. 2003
    ....
  49. pmc T-lymphoid, megakaryocyte, and granulocyte development are sensitive to decreases in CBFbeta dosage
    Laleh Talebian
    Division of Hematology Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    Blood 109:11-21. 2007
    ..Our data define a critical CBFbeta threshold for normal T-cell development, and situate an essential role for core-binding factors during the earliest stages of T-cell development...
  50. pmc Translational control of SCL-isoform expression in hematopoietic lineage choice
    Cornelis F Calkhoven
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Genes Dev 17:959-64. 2003
    ..In comparison, full-length SCL proteins are more efficient at enhancing the megakaryocyte lineage. Together, our studies unravel translational control as a novel mechanism regulating hematopoietic outcome...
  51. ncbi Obligate role of anti-apoptotic MCL-1 in the survival of hematopoietic stem cells
    Joseph T Opferman
    Howard Hughes Medical Institute, Department of Cancer Immunology and AIDS, Pathology and Medicine, Harvard Medical School, Boston, MA 02115, USA
    Science 307:1101-4. 2005
    ..Deletion of Mcl-1 in other tissues, including liver, did not impair survival. Thus, MCL-1 is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors...
  52. ncbi Cryopreservation of human embryonic stem cells without the use of a programmable freezer
    Sung Yun Ha
    Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, 110 744, Korea
    Hum Reprod 20:1779-85. 2005
    ..The aim of this study was to find an optimal cryopreservation protocol for human ES cells using slow freezing-rapid thawing without a programmable freezer...
  53. ncbi Primary murine MSC show highly efficient homing to the bone marrow but lose homing ability following culture
    W J C Rombouts
    Department of Haematology, Erasmus Medical Center, Rotterdam, The Netherlands
    Leukemia 17:160-70. 2003
    ..These data suggest that in vitro propagation of BM-derived MSC dramatically decreases their homing to BM and spleen...
  54. ncbi Reduced lymphomyeloid repopulating activity from adult bone marrow and fetal liver of mice lacking expression of STAT5
    Kevin D Bunting
    Hematopoiesis Department, American Red Cross Holland Laboratory, Rockville, MD, USA
    Blood 99:479-87. 2002
    ..These results demonstrate that a significant proportion of the growth factor signals required for multilineage reconstitution potential of HSCs is STAT5 dependent...
  55. ncbi The AML1 transcription factor functions to develop and maintain hematogenic precursor cells in the embryonic aorta-gonad-mesonephros region
    Y Mukouyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, 113, Japan
    Dev Biol 220:27-36. 2000
    ..5-dpc period. All these results support the notion that the AML1 transcription factor functions to develop and maintain hematogenic precursor cells in the embryonic P-Sp/AGM region...
  56. pmc Differential requirements for survivin in hematopoietic cell development
    Sandeep Gurbuxani
    The Ben May Institute for Cancer Research and Department of Pathology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 102:11480-5. 2005
    ..Together, these studies show that whereas survivin expression is essential for megakaryocyte and erythroid progenitors, its down-regulation is required for terminal differentiation of megakaryocytes...
  57. pmc Functional antagonism of the Polycomb-Group genes eed and Bmi1 in hemopoietic cell proliferation
    J Lessard
    Laboratory of Molecular Genetics of Hemopoietic Stem Cells, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
    Genes Dev 13:2691-703. 1999
    ..These studies thus suggest that hemopoietic cell proliferation is regulated by the relative contribution of repressive (Eed-containing) and enhancing (Bmi1-containing) PcG gene complexes...
  58. pmc Yersinia pseudotuberculosis disseminates directly from a replicating bacterial pool in the intestine
    Penelope D Barnes
    Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, MA 02111, USA
    J Exp Med 203:1591-601. 2006
    ..pseudotuberculosis to successfully establish replication in the intestinal lumen and does not result from ordered spread leading from the intestine to regional lymph nodes before dissemination...
  59. ncbi Different biological properties of circulating and bone marrow endothelial progenitor cells in acute myocardial infarction rats
    Z Xin
    The Division of Cardiology, Shanghai Sixth Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
    Thorac Cardiovasc Surg 56:441-8. 2008
    ..Many studies have shown that endothelial progenitor cell (EPC) can enhance the neovascularization of the ischemic myocardium. Peripheral blood and bone marrow are the most convenient resources for EPC...
  60. pmc Identification of a nonsense mutation in the granulocyte-colony-stimulating factor receptor in severe congenital neutropenia
    F Dong
    Dr Daniel den Hoed Cancer Center, Department of Hematology, Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 91:4480-4. 1994
    ..The mutant receptor chain may act in a dominant negative manner to block granulocytic maturation...
  61. ncbi Decreased number and impaired angiogenic function of endothelial progenitor cells in patients with chronic renal failure
    Jin Ho Choi
    Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
    Arterioscler Thromb Vasc Biol 24:1246-52. 2004
    ..We hypothesized that altered EPC biology may contribute to the pathophysiology of CRF...
  62. ncbi Validation and development of a predictive paradigm for hemotoxicology using a multifunctional bioluminescence colony-forming proliferation assay
    Ivan N Rich
    HemoGenix, Inc, Colorado Springs, 80907, USA
    Toxicol Sci 87:427-41. 2005
    ..When performed during early drug screening, the prediction value of the assay should help reduce time and cost, but above all, provide increase efficacy and safety for the patient...
  63. ncbi Hematopoietic-repopulating defects from STAT5-deficient bone marrow are not fully accounted for by loss of thrombopoietin responsiveness
    Heath L Bradley
    Hematopoiesis Department, Jerome H Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, MD 20855, USA
    Blood 103:2965-72. 2004
    ..These results indicate substantial overlap in c-Mpl-STAT5 signaling defects at the MPP/HSC level but indicate that STAT5 is activated independent of c-Mpl to promote multilineage hematopoietic differentiation...
  64. ncbi Fibroblast growth factor-1 and -2 preserve long-term repopulating ability of hematopoietic stem cells in serum-free cultures
    Joyce S G Yeoh
    Department of Cell Biology, Section Stem Cell Biology, University Medical Centre Groningen, Antonius, The Netherlands
    Stem Cells 24:1564-72. 2006
    ..Importantly, although HSC activity is typically rapidly lost after short-term culture in vitro, our current protocol allows us to sustain stem cell repopulation potential for periods up to 5 weeks...
  65. ncbi In vitro hematotoxicity testing in drug development: a review of past, present and future applications
    Ivan N Rich
    HemoGenix LLC, PO Box 1093, Irmo, SC 29063, USA
    Curr Opin Drug Discov Devel 6:100-9. 2003
    ..Chronotoxicology and chronotherapeutics constitute a special application, and can predict the best time of the day to administer anticancer drugs...
  66. ncbi Efficient mobilization of haematopoietic progenitors after a single injection of pegylated recombinant human granulocyte colony-stimulating factor in mouse strains with distinct marrow-cell pool sizes
    G de Haan
    Department of Cell Biology, University of Groningen, Groningen, The Netherlands
    Br J Haematol 110:638-46. 2000
    ..Comparison of the AKXL strain distribution pattern for marrow cellularity with loci previously mapped in these strains showed complete concordance with Aat, a serine protease inhibitor mapping to chromosome 12...
  67. ncbi Depletion of endothelial progenitor cells in the peripheral blood of patients with rheumatoid arthritis
    Johannes Grisar
    Division of Rheumatology, Department of Internal Medicine III, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Circulation 111:204-11. 2005
    ..Because the quantity of endothelial progenitor cells (EPCs) in the peripheral blood is correlated inversely with cardiovascular risk, we studied whether such abnormalities could also be observed in patients with RA...
  68. ncbi Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins
    T Papayannopoulou
    Division of Hematology, University of Washington, Seattle 98195 7710, USA
    Blood 98:2403-11. 2001
    ....
  69. ncbi Circulating endothelial progenitor cells predict coronary artery disease severity
    Geoffrey A Kunz
    Duke University Medical Center, Durham, NC 27710, USA
    Am Heart J 152:190-5. 2006
    ..Previous studies have shown an association between EPC numbers and the presence of traditional coronary artery disease (CAD) risk factors. The relationship between EPC numbers and the severity of atherosclerosis is, however, not known...
  70. pmc Differential expression of homeobox genes in functionally distinct CD34+ subpopulations of human bone marrow cells
    G Sauvageau
    Terry Fox Laboratory, British Columbia Cancer Agency, Canada
    Proc Natl Acad Sci U S A 91:12223-7. 1994
    ..These results demonstrate the expression of a broad range of Hox genes in primitive hematopoietic cells and point to the existence of a regulated program of Hox gene expression during their normal development...
  71. ncbi Reduced circulating angiogenic cells in Alzheimer disease
    S T Lee
    Department of Neurology, Seoul National University Hospital, 101, Daehangno, Jongno gu, Seoul 110 744, South Korea
    Neurology 72:1858-63. 2009
    ..To study the involvement of CACs in AD pathogenesis, we investigated the levels of CACs in patients with AD...
  72. ncbi Normal human bone marrow CD34(+)CD133(+) cells contain primitive cells able to produce different categories of colony-forming unit megakaryocytes in vitro
    Sabine Charrier
    Secteur d Etude en Hématopoi èse SEHM, Laboratoire d Hematologie, Faculte de Medecine et de Pharmacie, Clermont Ferrand Cedex, France
    Exp Hematol 30:1051-60. 2002
    ....
  73. ncbi Enhancement of long-term proliferative capacity of rabbit corneal epithelial cells by embryonic stem cell conditioned medium
    Ying Liu
    State Key Laboratory of Ophthalmology, Sun Yat Sen University, Guangzhou, P R China
    Tissue Eng Part C Methods 16:793-802. 2010
    ..Our findings indicate that this new ESC-CM culture system can generate low-immunogenic autologous cells sufficiently for use in regenerative medicine...
  74. ncbi Transgenic expression of stromal cell-derived factor-1/CXC chemokine ligand 12 enhances myeloid progenitor cell survival/antiapoptosis in vitro in response to growth factor withdrawal and enhances myelopoiesis in vivo
    Hal E Broxmeyer
    Department of Microbiology Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Immunol 170:421-9. 2003
    ..These results substantiate survival effects of SDF-1/CXCL12, now extended to progenitors engineered to endogenously produce low levels of this cytokine, and demonstrate activity in vivo for SDF-1/CXCL12 in addition to cell trafficking...
  75. ncbi Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation
    D G Phinney
    Center for Gene Therapy, MCP Hahnemann University, Philadelphia, Pennsylvania 19102, USA
    J Cell Biochem 72:570-85. 1999
    ..Our results point to significant strain differences in the properties of MSCs and indicate that standard methods cannot be applied to murine bone marrow to isolate relatively pure populations of MSCs...
  76. ncbi Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers
    James B Mitchell
    Cognate Therapeutics, Inc, Baltimore, MD, USA
    Stem Cells 24:376-85. 2006
    ....
  77. ncbi Bipotential B-macrophage progenitors are present in adult bone marrow
    E Montecino-Rodriguez
    Department of Pathology and Laboratory Medicine and the Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, CA 90095-1732, USA
    Nat Immunol 2:83-8. 2001
    ..In addition to the definition of this B-lineage intermediate, the data indicate that a developmental relationship between the B and macrophage lineages is retained during postnatal hematopoiesis...
  78. ncbi Overexpression of HOXA10 perturbs human lymphomyelopoiesis in vitro and in vivo
    C Buske
    The Terry Fox Laboratory, British Columbia Cancer Agency, and the Departments of Medicine and Medical Genetics, University of British Columbia, Vancouver, BC, Canada
    Blood 97:2286-92. 2001
    ..These results also provide a framework to initiate more detailed analyses of HOX regulatory domains and HOX cofactors in the human system in vitro and in vivo...
  79. ncbi Regulation of hemangioblast development
    G Lacaud
    Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, Box 1496, 1425 Madison Avenue, New York, New York 10029-6514, USA
    Ann N Y Acad Sci 938:96-107; discussion 108. 2001
    ..In addition, they highlight the strength of this model system in studying the function of genes in embryonic development...
  80. ncbi Isolation and characterization of canine hematopoietic progenitor cells
    G P Niemeyer
    Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Ala, USA
    Exp Hematol 29:686-93. 2001
    ..The development of methods for purification and characterization of canine hematopoietic progenitor cells should enhance the utilization of the canine model for a variety of experimental and therapeutic purposes...
  81. ncbi The relative quiescence of hematopoietic stem cells in nonhuman primates
    N Mahmud
    Hematology/Oncology Section and Transplantation Surgery Section, Biologic Resources Laboratory, University of Illinois College of Medicine, Chicago 60607-7173, USA
    Blood 97:3061-8. 2001
    ..The relative quiescence of PHSCs observed in this nonhuman primate model, in contrast to murine PHSCs, might explain the current barriers to genetic modification and ex vivo expansion of human PHSCs...
  82. ncbi tat protein stimulates production of transforming growth factor-beta 1 by marrow macrophages: a potential mechanism for human immunodeficiency virus-1-induced hematopoietic suppression
    G Zauli
    Institute of Microbiology, University of Bologna, Italy
    Blood 80:3036-43. 1992
    ....
  83. ncbi Local environmental factors determine hematopoietic differentiation of neural stem cells
    S Filip
    Department of Oncology and Radiotherapy, Charles University Hospital, 500 05 Hradec Kralove, Czech Republic
    Stem Cells Dev 13:113-20. 2004
    ..We also conclude that the bone marrow does not provide an environment supportive of hematopoietic differentiation by neural stem cells...
  84. ncbi Identification of hematopoietic stem/progenitor cells: strength and drawbacks of functional assays
    Laure Coulombel
    INSERM U 421, Faculte de Medecine, 8 rue du General Sarrail, Creteil 94010, France
    Oncogene 23:7210-22. 2004
    ..Nevertheless, years of progress in the manipulation of hematopoietic stem cells have recently resulted in the purification of a cell subset that repopulates irradiated recipients with absolute efficiency...
  85. ncbi [Effect of panaxadiol saponin and panaxtrol saponin on proliferation of human bone marrow hemopoietic progenitor cells]
    Yang Ping Niu
    College of Pharmacy, Zhejiang University of Technology, Hangzhou 310014
    Zhongguo Zhong Xi Yi Jie He Za Zhi 24:127-9, 132. 2004
    ..To observe the effect of panaxadiol saponin (PDS) and panaxtrol saponin (PTS) on proliferation of human bone marrow hemopoietic progenitor cells (HPC)...
  86. ncbi Stem cell factor and FLT3-ligand are strictly required to sustain the long-term expansion of primitive CD34+DR- dendritic cell precursors
    A Curti
    , University of Bologna, Bologna, Italy
    J Immunol 166:848-54. 2001
    ..Conversely, FLT3-L and SCF do not directly affect DC differentiation, but instead sustain the long-term expansion of CFU-DC, which can be induced to produce mature DC by GM-CSF and TNF-alpha...
  87. ncbi Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1)
    G Belaaloui
    Centre de Thérapie Cellulaire et Génique, Institut Paoli Calmettes, Centre Régional de Lutte Contre le Cancer Provence Alpes Côte d Azur, 232 Boulevard Sainte Marguerite, 13273 Marseille Cedex 9, France
    Leukemia 17:1137-45. 2003
    ..In conclusion, immature human hematopoietic progenitors express low levels of CD111 on their surface. During erythroid differentiation CD34+ cells acquire higher levels of the CD111 antigen...
  88. ncbi Hematopoietic defects in mice lacking the sialomucin CD34
    J Cheng
    Department of Molecular Oncology, Genentech, Inc, South San Francisco, CA, 94108, USA
    Blood 87:479-90. 1996
    ..However, the hematopoietic sites of adult CD34-deficient mice may still have a significant reservoir of progenitor cells that allows for normal recovery after nonmyeloablative peripheral cell depletion...
  89. ncbi Implication of a new molecule IK in CD34+ hematopoietic progenitor cell proliferation and differentiation
    L X Cao
    Division of Hematology, State University of New York, Stony Brook, USA
    Blood 89:3615-23. 1997
    ....
  90. pmc Transcription elongation factor S-II is required for definitive hematopoiesis
    Takahiro Ito
    Division of Developmental Biochemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Hongo 7 3 1, Bunkyo, Tokyo 113 0033
    Mol Cell Biol 26:3194-203. 2006
    ..Thus, S-II has critical and nonredundant roles in definitive hematopoiesis...
  91. pmc Novel role of interleukin 7 in myelopoiesis: stimulation of primitive murine hematopoietic progenitor cells
    F W Jacobsen
    Department of Immunology, Norwegian Radium Hospital, Montebello, Oslo, Norway
    J Exp Med 178:1777-82. 1993
    ..Thus, in addition to its established lymphopoietic potential, this study implicates a novel role of IL-7 in early myelopoiesis...
  92. ncbi Efficient hematopoietic differentiation of human embryonic stem cells on stromal cells derived from hematopoietic niches
    Maria H Ledran
    North East Institute for Stem Cell Research, Newcastle University, Newcastle upon Tyne, UK
    Cell Stem Cell 3:85-98. 2008
    ..Overall, our findings represent significant progress toward the goal of deriving functional hematopoietic stem cells from hESCs...
  93. ncbi Concise review: hematopoietic stem cells and tissue stem cells: current concepts and unanswered questions
    Donald Metcalf
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    Stem Cells 25:2390-5. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  94. ncbi Transduction of human hematopoietic stem cells by lentiviral vectors pseudotyped with the RD114-TR chimeric envelope glycoprotein
    F Di Nunzio
    MolMed, 20132 Milan, Italy
    Hum Gene Ther 18:811-20. 2007
    ..RD114-TR is compatible with the development of lentiviral stable packaging cell lines, and may become the envelope of choice for clinical studies aiming at safe and efficient genetic modification of human hematopoietic stem cells...
  95. ncbi Mesenchymal stromal cells from umbilical cord blood
    Karen Bieback
    Institute of Transfusion Medicine and Immunology, German Red Cross Blood Donor Service Baden Wuerttemberg Hessen gGmbH, Medical Faculty Mannheim, University of Heidelberg, Germany
    Curr Stem Cell Res Ther 2:310-23. 2007
    ....
  96. pmc Genetic control of the frequency of hematopoietic stem cells in mice: mapping of a candidate locus to chromosome 1
    C E Muller-Sieburg
    Medical Biology Institute, La Jolla, California 92037, USA
    J Exp Med 183:1141-50. 1996
    ..We have named this gene Scfr1 (stem cell frequency regulator 1). The allelic forms of this gene may be an important predictor of stem cell number and thus would be useful for evaluating cell sources in clinical stem cell transplantation...
  97. ncbi CD34(+) cord blood cells expressing cutaneous lymphocyte-associated antigen are enriched in granulocyte-macrophage progenitors and support extensive amplification of dendritic cell progenitors
    J F Arrighi
    Allergology Unit, Division of Immunology and Allergology, Geneva University Hospital, Geneva, Switzerland
    Exp Hematol 29:1029-37. 2001
    ..In addition, cultures of CD34(+)CLA(+) cells from cord blood produced CD34(+)CLA(-) cells, suggesting that these cells may derive directly from CD34(+)CLA(+) cells in vivo...
  98. ncbi Persisting multilineage transgene expression in the clonal progeny of a hematopoietic stem cell
    Z Li
    Experimental Cell Therapy, Department of Hematology and Oncology, Hannover Medical School, Hannover, Germany
    Leukemia 16:1655-63. 2002
    ....
  99. ncbi Possibility of progenitor cell mobilization during the hematological recovery following peripheral blood stem cell autograft
    Maria Stefania De Propris
    Dipartimento di Biotecnologia Cellulari ed Ematologia, Universita La Sapienza, Roma, Italia
    Acta Haematol 109:57-63. 2003
    ..However, when the clonogenic potential of a single CD34+ cell was evaluated, no major differences in the number of colonies produced per CD34+ cell were found between the two groups...
  100. ncbi Cellular copper content modulates differentiation and self-renewal in cultures of cord blood-derived CD34+ cells
    Tony Peled
    Gamida Cell Ltd, Jerusalem, Israel
    Br J Haematol 116:655-61. 2002
    ..This novel method of controlling cell proliferation and differentiation by copper and copper chelators might be utilized for ex vivo manipulation of HPC for various clinical applications...
  101. ncbi Transduced CD34+ cells from adrenoleukodystrophy patients with HIV-derived vector mediate long-term engraftment of NOD/SCID mice
    Sonia Benhamida
    INSERM U561, Hopital Saint Vincent de Paul, 75014 Paris, France
    Mol Ther 7:317-24. 2003
    ..Transplantation of transduced ALD CD34(+) cells into NOD/SCID mice resulted in long-term expression of ALD protein in monocytes/macrophages derived from engrafted stem cells...

Research Grants64

  1. Endothelial Progenitor Cells in Brain Arteriovenous Malformations
    Yongmei Chen; Fiscal Year: 2010
    ..EPC function will be determined by colony forming units assay. The primary outcome will be EPC number, function and plasma VEGF level...
  2. Reprogramming committed blood cells to a hematopoietic stem cell fate
    Derrick J Rossi; Fiscal Year: 2013
    ..In terms of clinical application, if successful, our study opens the possibility of eventually generating patient- specific induced HSCs for use in bone marrow transplantation, or for the study of blood disorders. ..
  3. CONTROL OF HEMATOPOIETIC TOXICITY WITH INTERLEUKIN-1
    VINCENT GALLICCHIO; Fiscal Year: 1990
    ..Toward this long-term goal to develop a specific agent(s) that may either enhance the recovery of blood cell production or reduce hematopoietic damage as the result of chemotherapy, therefore, may have important clinical applications...
  4. Multivariate Analysis for Molecular Toxicology Screening
    Seth Michelson; Fiscal Year: 2002
    ..proposed commercial application: ..
  5. Optimizing Assays of Human Stem Cells in Bone Marrow
    George Muschler; Fiscal Year: 2007
    ..abstract_text> ..
  6. BASIC STUDIES OF EXPERIMENTAL TUMOR MODELS
    Robert Sutherland; Fiscal Year: 1992
    ....
  7. Age-Related Changes in Epidermal Stem Cells
    Ruby Ghadially; Fiscal Year: 2001
    ..abstract_text> ..
  8. Epidermal Stem Cells: Age-Related Changes
    Ruby Ghadially; Fiscal Year: 2004
    ..abstract_text> ..
  9. CHEMOKINES/CYTOKINES AND RECEPTORS IN STEM CELL HOMING
    Hal Broxmeyer; Fiscal Year: 2005
    ..These studies should clarity the relevance of SDF-1 and CXCR4 for homing/engraftment and mobilization of primary stem/progenitor cells. ..
  10. GLOBIN SWITCHING AND ERYTHROID DIFFERENTIATION
    Thalia Papayannopoulou; Fiscal Year: 2002
    ....
  11. BIOLOGICAL PROPERTIES AND REGULATION OF STEM CELLS
    Thalia Papayannopoulou; Fiscal Year: 2009
    ..We believe that the proposed studies will enhance our understanding and provide a more comprehensive picture of the influence of <x4 integrins on prenatal and postnatal hematopoiesis. ..
  12. Stem Cell Assays
    Mark Chee; Fiscal Year: 2008
    ..In the longer term, pluripotent cells have the potential to be used in regenerative medicine, which will require reliable assays for pluripotence. [unreadable] [unreadable] [unreadable]..
  13. Ras-PI3K Pathway in Nf1-/-Hematopoiesis and Leukemia
    David Ingram; Fiscal Year: 2007
    ..abstract_text> ..
  14. Program Project Grant-Pathobiology of Paget's Disease
    G Roodman; Fiscal Year: 2007
    ..The knowledge gained from this P01 grant will offer important insights for normal bone cell biology especially for understanding the paracrine regulation of osteoblasts by OCLs. ..
  15. The Role of MLL-AF9 in Acute Myeloid Leukemia
    James C Mulloy; Fiscal Year: 2010
    ..RELEVANCE (See instructions): We have recently shown that human blood stem cells can be induced to make leukemia upon introduction ..
  16. Regulation of HSC self-renewal by morphogens
    LOUISE PURTON; Fiscal Year: 2007
    ..Our data suggest that a vitamin A compound may be very useful in improving both of these approaches. [unreadable] [unreadable] [unreadable]..
  17. Transformation of Cells by the REL Oncogene
    Thomas Gilmore; Fiscal Year: 2009
    ....
  18. RUNX-fusion Target Genes in Normal and Leukemic Hematopoiesis
    JAMES MULLOY; Fiscal Year: 2007
    ..Understanding the modulation of the self-renewal process by these fusion proteins could give insight into the normal mechanisms of self-renewal employed by the stem cell. [unreadable] [unreadable]..
  19. Conference on immune suppression in cancer
    Dmitry Gabrilovich; Fiscal Year: 2007
    ..tumor escape of immune system control; [unreadable] Review and discuss how our knowledge of new mechanisms of tumor associated immune suppression can be translated into new therapeutics [unreadable] [unreadable] [unreadable] [unreadable]..
  20. Development of SL-101, An Immunotoxin that Targets Cancer Stem Cells
    Malcolm Moore; Fiscal Year: 2006
    ..This Phase I STTR grant will cover the development of SL-101 through key phases of pre-clinical development. [unreadable] [unreadable] [unreadable]..
  21. Paget's Disease of Bone/Dysplasia:Advances/Challenges
    G Roodman; Fiscal Year: 2006
    ..to become aware of the effectiveness of the various drugs that can be used to treat the two disorders; 5.) to understand the role of surgery in managing the two disorders. ..
  22. Analysis of CD81-PGRL complex on T cells
    STEPHEN CHAPES; Fiscal Year: 2007
    ..The coordinated effect of CD81, PGRL and EP3 on T cell integrin activity measured by adhesion to or motility on ICAMs. ..
  23. P53 Based Vaccine for Small Cell Lung Cancer
    Dmitry Gabrilovich; Fiscal Year: 2007
    ..Specific Aim 2. Determine the immune modifying effect of immunization and chemotherapy on p53-specific immunity. [unreadable] [unreadable] [unreadable]..
  24. NATIONAL GENE VECTOR LABORATORY AT INDIANA UNIVERSITY
    Kenneth Cornetta; Fiscal Year: 2007
    ..abstract_text> ..
  25. Role of Placenta Growth factor in Sickle ACS
    MALIK PUNAM; Fiscal Year: 2008
    ..VEGFR-antagonists are in clinical trials and leukotriene blockers are FDA approved and in clinical use for asthma and could be candidates for an ACS prevention trial. ..
  26. Cord Blood Expansion and Transplantation in a Nonhuman Primate Model
    Hans Peter Kiem; Fiscal Year: 2009
    ....
  27. OSTEOCLASTS FROM TRANSGENIC MICE
    G Roodman; Fiscal Year: 2009
    ..Our long-term goal will be to characterize the role of ADAM8/a9b1 in the bone destruction which accompanies collagen induced arthritis, which will be started in grant year 5. ..
  28. Fox Chase Cancer Center Breast Cancer Tissue Resources
    Jose Russo; Fiscal Year: 2008
    ..abstract_text> ..
  29. Interaction of AIDS-associated microbes with radiation
    Ekaterina Dadachova; Fiscal Year: 2009
    ..Such knowledge will contribute to development of a novel therapeutic modality based on radioimmunotherapy for treatment of currently incurable or multidrug-resistant AIDS-associated opportunistic infections. ..
  30. Gene therapy for Fanconi anemia
    Hans Peter Kiem; Fiscal Year: 2009
    ..Our ultimate goal is to improve or cure the hematopoietic symptoms in FA patients by lentivirus-mediated HSC gene transfer, and develop methodologies that would be applicable to other genetic disorders and hematological diseases. ..
  31. Genomic Markers of Breast Cancer Prevention Induced by hCG in Women at High Risk
    Jose Russo; Fiscal Year: 2008
    ..This knowledge will serve as the basis for establishing novel genomic signatures as intermediate biomarkers for larger preventive clinical trials at the completion of this project. [unreadable] [unreadable] [unreadable]..
  32. Genomic analyses of autism spectrum disorders
    VALERIE HU; Fiscal Year: 2008
    ..Reliable biomarkers would greatly facilitate the early and definitive detection of these disorders, thereby permitting early intervention and therapy. [unreadable] [unreadable] [unreadable]..
  33. Tissue regenerative capacity of epidermal progenitors
    Pritinder Kaur; Fiscal Year: 2008
    ..The proposed studies may also provide a means for expanding all keratinocyte progenitors by manipulation of growth factors and extracellular matrix proteins. ..
  34. The Role of CBFb-MYH11 in Acute Myeloid Leukemia
    James C Mulloy; Fiscal Year: 2010
    ..To identify specific signals that will cooperate in the transformation to acute leukemia, defined eenetic elements and saturatine retroviral mutaeenesis will be used. ..
  35. In Utero Transplantation in Primates
    Robert Andrews; Fiscal Year: 2008
    ..If our hypotheses are correct, then in utero treatment for hematopoietic and immune disorders should be both safer and successful. [unreadable] [unreadable]..
  36. Proteasome Inhibition in Myelodysplastic Syndromes
    Jane Liesveld; Fiscal Year: 2006
    ..These studies will define a potential therapeutic role for bortezomib in MDS and will aid in understanding the hematopoietic stem cell in these disorders. ..
  37. First Int'l Symposium on Childhood and Adolescent NHL
    Mitchell Cairo; Fiscal Year: 2003
    ..abstract_text> ..
  38. Nonablative AlloSCT in Refractory Autoimmune Disease
    Mitchell Cairo; Fiscal Year: 2003
    ....
  39. TRAUMA-RELATED ERYTHROPOIETIC SUPPRESSION
    David Livingston; Fiscal Year: 2003
    ..Understanding the mechanisms behind post-trauma anemia will allow therapeutic interventions improving endogenous erythropoiesis to be targeted. ..
  40. BCG + hsp, a New Tuberculosis Vaccine
    Boris Nikonenko; Fiscal Year: 2003
    ..We will also examine the antibiotic sensitivity of this rBCGdeltahspR to 10 commonly used TB drugs to ensure that the genetic manipulations performed have not made it less sensitive to drug treatment. ..
  41. Gene Gun Technology, Opioids, and Corneal Diseases
    IAN ZAGON; Fiscal Year: 2003
    ..Ultimately, such data can be employed to design molecular strategies to remedy visual dysfunction. ..
  42. BREAST CANCER/BONE MARROW STROMAL INTERACTIONS
    Pranela Rameshwar; Fiscal Year: 2003
    ..Since BC metastasis involves two major cell subsets, mesenchymal (stroma) and epithelial (BC), aim 4 will determine if regulation of PPT-I, NK-1 and NK-2 is tissue specific by studying their promoters in these cell subsets. ..
  43. OXIDATIVE STRESS, APOPTOSIS AND DRUG RESISTANCE
    Istvan Boldogh; Fiscal Year: 2003
    ..abstract_text> ..
  44. IMMUNOLOGICAL CONSEQUENCES OF TRANSFUSION
    Loren Fast; Fiscal Year: 2002
    ..abstract_text> ..
  45. MECHANISM(S) OF CHEMICAL LEUKEMOGENESIS
    Richard Irons; Fiscal Year: 2002
    ..A. in specific sub-populations of CD34+ human BM cells. Finally, patterns of gene expression will be correlated with genotype, phenotype and functional clonogenic response in BM cells obtained from patients with MDS or evolving sAML. ..
  46. FACTORS INFLUENCING STEM CELL AND PLATELET PRODUCTION
    Malcolm Moore; Fiscal Year: 2002
    ..e., adult populations acquiring features of neonatal cells, or whether there is a tendency for "immortalized" cells to undergo neoplastic transformation. ..
  47. The Role of Chemokines in Metastatic Prostate Cancer
    Russell Taichman; Fiscal Year: 2003
    ..abstract_text> ..
  48. Redox Control of Radiation Response
    John Biaglow; Fiscal Year: 2006
    ..Ultimately, we hope to develop specific strategies for modifying thiol redox homeostasis in vivo, to make human tumors more sensitive to ionizing radiation. ..
  49. BUILDING RESEARCH CAPACITY IN THE SCIENCES
    Veronica Nwosu; Fiscal Year: 2006
    ..abstract_text> ..
  50. Genomic Basis of Reproductive History on Breast Cancer
    Jose Russo; Fiscal Year: 2006
    ..abstract_text> ..
  51. MGMT-Mediated in vivo Selection in a Large Animal Model
    Hans Peter Kiem; Fiscal Year: 2006
    ..These experiments will provide a platform for studies using gene-modified allogeneic stem cells to improve nonmyeloablative transplant strategies. ..
  52. SIGNALING PATHWAYS MEDIATING TROPHOBLAST PROLIFERATION
    Daniel Rappolee; Fiscal Year: 2005
    ..Therefore, the proposed studies should lead to improvement in methods to facilitate placentation and improve IVF and produce protocols for remediation of common defects in pregnancy such as pre-eclampsia and IUGR. ..
  53. VEGF and bone remodeling in skeletal metastases
    Evan Keller; Fiscal Year: 2005
    ..In this aim, we will test which VEGF isoform contributes to CaP cells' osteoblastic activity. We will also evaluate if VEGF induces this activity through MAPK. ..
  54. Flavopiridol as a Potential Therapy in Multiple Myeloma
    Keith Bible; Fiscal Year: 2005
    ..Results of proposed studies will improve understanding of the effects and mechanism(s) of action of flavopiridol in myeloma cells, thereby potentially contributing to the further clinical development of the drug. ..
  55. Role of AML1/ETO in Hematopoiesis and Leukemogenesis
    JAMES MULLOY; Fiscal Year: 2005
    ..The establishment of a small animal model of AML will greatly enhance our ability to develop and test treatment strategies and drugs that may be useful in the therapy of AML. ..
  56. ROLE OF GRP78 IN ALKYLATING AGENT SENSITIVITY
    Satadal Chatterjee; Fiscal Year: 2005
    ....
  57. NEUTROPHIL RECRUITMENT IN LPS-INDUCED AIRWAY DISEASE
    Jessica Moreland; Fiscal Year: 2004
    ....
  58. Bone and the Hematopoietic System
    Russell Taichman; Fiscal Year: 2004
    ..These investigations will ultimately lead to new strategies for the treatment of hematopoietic and osseous disorders. ..
  59. Vaccine hurdle to anthrax and the emerging immune system
    Pranela Rameshwar; Fiscal Year: 2006
    ..This study will also form the basis to develop in vivo model with human hematopoietic system to study anthrax infection and also to improve responses to anthrax vaccines. ..
  60. CELLULAR AND MOLECULAR BASIS OF HIV BASED THROMBOCYTOPEN
    Mariusz Ratajczak; Fiscal Year: 2002
    ..In toto, the studies proposed in this grant will increase the knowledge about pathogenesis of AIDS associated thrombocytopenia and may lead to development of new strategies for its treatment of prevention. (End of Abstract) ..
  61. RESEARCH TRAINING IN TRANSFUSION MEDICINE
    Jeffrey McCullough; Fiscal Year: 2003
    ..abstract_text> ..
  62. Origin of HSC from Embryonic Stem Cells
    Elias Zambidis; Fiscal Year: 2008
    ..The success of these goals will lay the groundwork for characterizing human stem cells not available by any other means. ..
  63. The CXCR4-SDF-1 Axis in Metastatic Rhabdomyosarcoma
    Mariusz Ratajczak; Fiscal Year: 2009
    ..This work will be of relevance for designing strategies to block the SDF-1-CXCR4 axis to treat CXCR4-positive cancers. ..