cell fractionation

Summary

Summary: Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.

Top Publications

  1. ncbi Contamination of nuclear fractions with plasma membrane lipid rafts
    Yee How Say
    Proteolysis Research Group, Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK
    Proteomics 7:1059-64. 2007
  2. pmc Characterization of a cis-Golgi matrix protein, GM130
    N Nakamura
    Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK
    J Cell Biol 131:1715-26. 1995
  3. pmc A v-SNARE implicated in intra-Golgi transport
    M Nagahama
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York 10021, USA
    J Cell Biol 133:507-16. 1996
  4. pmc Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking
    T K Sato
    Division of Cellular and Molecular Medicine and Department of Biology, Howard Hughes Medical Institute, University of California at San Diego School of Medicine, La Jolla, California 92093 0668, USA
    Mol Cell Biol 18:5308-19. 1998
  5. pmc Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes
    Rachel S Lerner
    Departments of Cell Biology and Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 9:1123-37. 2003
  6. ncbi DNA-dependent protein kinase and XRCC4-DNA ligase IV mobilization in the cell in response to DNA double strand breaks
    Jerome Drouet
    Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077 Toulouse, Cedex 4, France
    J Biol Chem 280:7060-9. 2005
  7. ncbi Glucose transport and system A activity in syncytiotrophoblast microvillous and basal plasma membranes in intrauterine growth restriction
    T Jansson
    Perinatal Center, Department of Physiology and Pharmacology, Goteborg University, Sweden
    Placenta 23:392-9. 2002
  8. pmc Regulation of Jak2 through the ubiquitin-proteasome pathway involves phosphorylation of Jak2 on Y1007 and interaction with SOCS-1
    Daniela Ungureanu
    Institute of Medical Technology, University of Tampere, FIN 33014 Tampere, Finland
    Mol Cell Biol 22:3316-26. 2002
  9. ncbi Nesprin-2 is a multi-isomeric protein that binds lamin and emerin at the nuclear envelope and forms a subcellular network in skeletal muscle
    Qiuping Zhang
    Department of Medicine, ACCI, Box 110, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QQ, UK
    J Cell Sci 118:673-87. 2005
  10. pmc Coupled ER to Golgi transport reconstituted with purified cytosolic proteins
    C Barlowe
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Cell Biol 139:1097-108. 1997

Research Grants

Detail Information

Publications211 found, 100 shown here

  1. ncbi Contamination of nuclear fractions with plasma membrane lipid rafts
    Yee How Say
    Proteolysis Research Group, Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK
    Proteomics 7:1059-64. 2007
    ....
  2. pmc Characterization of a cis-Golgi matrix protein, GM130
    N Nakamura
    Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK
    J Cell Biol 131:1715-26. 1995
    ..Together these results suggest that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure...
  3. pmc A v-SNARE implicated in intra-Golgi transport
    M Nagahama
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York 10021, USA
    J Cell Biol 133:507-16. 1996
    ..Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles...
  4. pmc Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking
    T K Sato
    Division of Cellular and Molecular Medicine and Department of Biology, Howard Hughes Medical Institute, University of California at San Diego School of Medicine, La Jolla, California 92093 0668, USA
    Mol Cell Biol 18:5308-19. 1998
    ....
  5. pmc Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes
    Rachel S Lerner
    Departments of Cell Biology and Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 9:1123-37. 2003
    ..In this report, we use multiple cell fractionation protocols, in combination with cDNA microarray, nuclease protection, and Northern blot analyses, to assess ..
  6. ncbi DNA-dependent protein kinase and XRCC4-DNA ligase IV mobilization in the cell in response to DNA double strand breaks
    Jerome Drouet
    Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077 Toulouse, Cedex 4, France
    J Biol Chem 280:7060-9. 2005
    ..Altogether these results offer insights into the interplay between key NHEJ proteins during this repair process in the cell...
  7. ncbi Glucose transport and system A activity in syncytiotrophoblast microvillous and basal plasma membranes in intrauterine growth restriction
    T Jansson
    Perinatal Center, Department of Physiology and Pharmacology, Goteborg University, Sweden
    Placenta 23:392-9. 2002
    ..The hypoglycemia present in utero in some IUGR fetuses is not caused by a decreased glucose transport capacity across the syncytiotrophoblast plasma membranes...
  8. pmc Regulation of Jak2 through the ubiquitin-proteasome pathway involves phosphorylation of Jak2 on Y1007 and interaction with SOCS-1
    Daniela Ungureanu
    Institute of Medical Technology, University of Tampere, FIN 33014 Tampere, Finland
    Mol Cell Biol 22:3316-26. 2002
    ....
  9. ncbi Nesprin-2 is a multi-isomeric protein that binds lamin and emerin at the nuclear envelope and forms a subcellular network in skeletal muscle
    Qiuping Zhang
    Department of Medicine, ACCI, Box 110, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2QQ, UK
    J Cell Sci 118:673-87. 2005
    ..Moreover, its association at the NE with lamin and emerin, the genes mutated in Emery-Dreifuss muscular dystrophy, suggests a mechanism to explain how disruption of the NE leads to muscle dysfunction...
  10. pmc Coupled ER to Golgi transport reconstituted with purified cytosolic proteins
    C Barlowe
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Cell Biol 139:1097-108. 1997
    ..Ordering experiments using the dilution resistant intermediate and reversible Sec23p complex inhibition indicate Sec18p action is required before LMA1 function...
  11. ncbi Selection of axial growth sites in yeast requires Axl2p, a novel plasma membrane glycoprotein
    T Roemer
    Department of Biology, Yale University, New Haven, Connecticut 96520, USA
    Genes Dev 10:777-93. 1996
    ..We suggest that Axl2p acts as an anchor in the plasma membrane that helps direct new growth components and/or polarity establishment components to the cortical axial budding site...
  12. ncbi Nuclear membrane proteins with potential disease links found by subtractive proteomics
    Eric C Schirmer
    Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 301:1380-2. 2003
    ..Furthermore, 23 of these mapped within chromosome regions linked to a variety of dystrophies...
  13. pmc Rapid accumulation of Akt in mitochondria following phosphatidylinositol 3-kinase activation
    Gautam N Bijur
    Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Neurochem 87:1427-35. 2003
    ..These results demonstrate that signals emanating from plasma membrane receptors or generated by stress rapidly modulate Akt and glycogen synthase kinase-3beta in mitochondria...
  14. pmc Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina
    A Senior
    Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Cell Biol 107:2029-36. 1988
    ..Therefore, these integral proteins are biochemical markers for the inner nuclear membrane and will be useful models for studying nuclear membrane biogenesis...
  15. ncbi Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes
    S Krajewski
    Oncogene and Tumor Suppression Gene Program, La Jolla Cancer Research Foundation, California 92037
    Cancer Res 53:4701-14. 1993
    ....
  16. ncbi Proteomics of organelles and large cellular structures
    John R Yates
    Department of Cell Biology, 10550 North Torrey Pines Road, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 6:702-14. 2005
    ..Here, we review the state-of-the-art proteomics techniques for the analysis of subcellular structures and discuss the biological insights that have been derived from such studies...
  17. ncbi Microtubules, tubulin, and microtubule-associated proteins of trypanosomes
    D Robinson
    Methods Enzymol 196:285-99. 1991
  18. ncbi Dynamic cell fractionation and transportation using moving dielectrophoresis
    Chin Hock Kua
    Singapore MIT Alliance, Nanyang Technological University, Singapore 639798
    Anal Chem 79:6975-87. 2007
    ..b>Cell fractionation is controlled by the applied electrical frequency, and cell transportation is controlled by the ..
  19. pmc A heterodimer of thioredoxin and I(B)2 cooperates with Sec18p (NSF) to promote yeast vacuole inheritance
    Z Xu
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    J Cell Biol 136:299-306. 1997
    ..Upon Sec17p/Sec18p action, vacuoles become labile but are rapidly stabilized by LMA1. The action of LMA1 and Sec18p is thus coupled and ordered. These data establish LMA1 as a novel factor in trafficking of yeast vacuoles...
  20. ncbi Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells
    B P Zhou
    Department of Molecular and Cellular Oncology, Breast Cancer Basic Research Program, University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 3:245-52. 2001
    ..Our results indicate that HER-2/neu induces cytoplasmic localization of p21Cip1/WAF1 through activation of Akt to promote cell growth, which may have implications for the oncogenic activity of HER-2/neu and Akt...
  21. ncbi The Hansenula polymorpha PDD1 gene product, essential for the selective degradation of peroxisomes, is a homologue of Saccharomyces cerevisiae Vps34p
    J A Kiel
    Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Kerklaan 30, NL 9751 NN Haren, The Netherlands
    Yeast 15:741-54. 1999
    ..cerevisiae vps34 mutants-H. polymorpha pdd1 mutants are temperature-sensitive for growth and are impaired in the sorting of vacuolar carboxypeptidase Y. Moreover, HpPdd1p is associated to membranes, as was also observed for ScVps34p...
  22. ncbi A mammalian PAR-3-PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity
    D Lin
    Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Nat Cell Biol 2:540-7. 2000
    ..In vitro, mPAR-3 acts as a substrate and an inhibitor of aPKC. We conclude that mPAR-3 and mPAR-6 have a scaffolding function, coordinating the activities of several signalling proteins that are implicated in mammalian cell polarity...
  23. pmc Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors
    Leonard J Foster
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Proc Natl Acad Sci U S A 100:5813-8. 2003
    ..Our results provide the first large-scale and unbiased evidence, to our knowledge, for the connection of rafts with signaling and place limits on the fraction of plasma membrane composed by rafts...
  24. pmc A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole
    S E Rieder
    Department of Biology, Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, La Jolla 92093 0668, USA
    Mol Biol Cell 8:2307-27. 1997
    ..Thus we propose that the class C Vps proteins are components of a hetero-oligomeric protein complex that mediates the delivery of multiple transport intermediates to the vacuole...
  25. pmc Nuclear envelope proteomics: novel integral membrane proteins of the inner nuclear membrane
    M Dreger
    Institute for Chemistry Biochemistry, Free University Berlin, Thielallee 63, D 14195 Berlin, Germany
    Proc Natl Acad Sci U S A 98:11943-8. 2001
    ..The utility of our approach is not restricted to the investigation of the NE. Our approach should be applicable to the analysis of other complex membrane structures of the cell as well...
  26. pmc Experimental analysis of the rice mitochondrial proteome, its biogenesis, and heterogeneity
    Shaobai Huang
    Australian Research Council Centre of Excellence in Plant Energy Biology, M316, University of Western Australia, Crawley, 6009 Western Australia, Australia
    Plant Physiol 149:719-34. 2009
    ....
  27. ncbi A novel action of histone deacetylase inhibitors in a protein aggresome disease model
    Lisa J Corcoran
    Institute of Chemistry and Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Curr Biol 14:488-92. 2004
    ..Our findings suggest a connection between HDAC activity and aggresome formation and also lay the groundwork for a direct test of the role of aggresome formation in ALS etiology...
  28. pmc Brittle-1, an adenylate translocator, facilitates transfer of extraplastidial synthesized ADP--glucose into amyloplasts of maize endosperms
    J C Shannon
    Department of Horticulture, 102 Tyson Building, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Plant Physiol 117:1235-52. 1998
    ..The importance of the BT1 translocator in starch accumulation in maize endosperms is demonstrated by the severely reduced starch content in bt1 mutant kernels...
  29. pmc High-resolution fractionation of signaling endosomes containing different receptors
    Gretchen McCaffrey
    Division of Biological Sciences, University of Montana, Missoula, Montana, USA
    Traffic 10:938-50. 2009
    ..Receptor-specific endosomes may direct the intracellular location and duration of signal transduction pathways to dictate response to signals and determine cell fate...
  30. ncbi Interactions of STAT3 with caveolin-1 and heat shock protein 90 in plasma membrane raft and cytosolic complexes. Preservation of cytokine signaling during fever
    Mehul Shah
    Department of Cell Biology and Anatomy, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 277:45662-9. 2002
    ..Taken together, the data show that IL-6/raft/STAT3 signaling is a chaperoned pathway that involves cav-1 and HSP90 as accessory proteins and suggest a mechanism for the preservation of this signaling during fever...
  31. pmc Transport of LDL-derived cholesterol from the NPC1 compartment to the ER involves the trans-Golgi network and the SNARE protein complex
    Yasuomi Urano
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 105:16513-8. 2008
    ..These results show that vesicular trafficking is involved in transporting a significant portion of LDL-CHOL from the NPC1-containing endosomal compartment to the TGN before its arrival at the ER...
  32. pmc Regulated binding of PTP1B-like phosphatase to N-cadherin: control of cadherin-mediated adhesion by dephosphorylation of beta-catenin
    J Balsamo
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    J Cell Biol 134:801-13. 1996
    ..In this manner the GalNAcPTase-proteoglycan interaction may play a major role in morphogenetic cell and tissue interactions during development...
  33. pmc Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression
    Maria Eugenia Zanetti
    Department of Botany and Plant Science, Center for Plant Cell Biology, University of California, Riverside, California 92521 0124, USA
    Plant Physiol 138:624-35. 2005
    ..This technology can be extended to evaluation of mRNA populations at the cell- or tissue-specific level by regulation of the tagged RP with distinct promoters...
  34. pmc An endosomal beta COP is involved in the pH-dependent formation of transport vesicles destined for late endosomes
    F Aniento
    Department of Biochemistry, Sciences II, Geneva, Switzerland
    J Cell Biol 133:29-41. 1996
    ....
  35. pmc Annexin II is a major component of fusogenic endosomal vesicles
    N Emans
    European Molecular Biology Laboratory, Heidelberg, Germany
    J Cell Biol 120:1357-69. 1993
    ..These data demonstrate that annexin II, together with the other four proteins we have identified, is a major component of fusogenic endosomal vesicles, suggesting that these proteins are involved in the binding and/or fusion process...
  36. pmc Transport of axl2p depends on erv14p, an ER-vesicle protein related to the Drosophila cornichon gene product
    J Powers
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Cell Biol 142:1209-22. 1998
    ..These results suggest an unforeseen conservation in mechanisms producing cell polarity shared between yeast and Drosophila...
  37. ncbi MglA and MglB are required for the intramacrophage growth of Francisella novicida
    G S Baron
    Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada
    Mol Microbiol 29:247-59. 1998
    ..novicida. mglB expression in GB2 was confirmed using antiserum against recombinant MglB. Cell fractionation studies revealed several differences in the protein profiles of mgI mutants compared with wild-type F...
  38. pmc Analysis of the compartmentation of glycolytic intermediates, nucleotides, sugars, organic acids, amino acids, and sugar alcohols in potato tubers using a nonaqueous fractionation method
    E M Farré
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Golm, Germany
    Plant Physiol 127:685-700. 2001
    ..However, due to the low plastidic pyrophosphate concentration, the reaction catalyzed by ADP-glucose pyrophosphorylase was estimated to be far removed from equilibrium...
  39. ncbi Subcellular fractionation of secretory organelles
    Chris A Kaiser
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Methods Enzymol 351:325-38. 2002
  40. ncbi Determination of subcellular concentrations of soluble carbohydrates in rose petals during opening by nonaqueous fractionation method combined with infiltration-centrifugation method
    Kunio Yamada
    National Institute of Floricultural Science, Fujimoto, Tsukuba, Ibaraki 305 8519, Japan
    Planta 230:1115-27. 2009
    ....
  41. ncbi Isolation of intact, functional mitochondria from the model plant Arabidopsis thaliana
    Lee J Sweetlove
    Department of Plant Sciences, University of Oxford, United Kingdom
    Methods Mol Biol 372:125-36. 2007
    ..We also present details of commonly used assays to assess the physical and functional integrity of the isolated organelles...
  42. ncbi Chromatin assembly in yeast cell-free extracts
    M C Schultz
    Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7
    Methods 17:161-72. 1999
    ..This simple system offers a unique opportunity to analyze chromatin metabolism by a combined biochemical and genetic approach that is not feasible for any other model organism...
  43. ncbi Changes in chloroplast DNA during development in tobacco, Medicago truncatula, pea, and maize
    Jeffrey M Shaver
    Department of Biology, University of Washington, 355325, Seattle, WA 98195 5325, USA
    Planta 224:72-82. 2006
    ....
  44. pmc The chlamydial plasmid-encoded protein pgp3 is secreted into the cytosol of Chlamydia-infected cells
    Zhongyu Li
    Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    Infect Immun 76:3415-28. 2008
    ....
  45. ncbi Identification of the universal cofactor (auxilin 2) in clathrin coat dissociation
    A Umeda
    Department of Cell Biology, Centre of Anatomy, Hannover Medical School, Germany
    Eur J Cell Biol 79:336-42. 2000
    ..The cellular distribution of membrane-associated adaptors was unaffected by overexpression of GAK. Our results point to a hsc70/auxilin-based uncoating system as a ubiquitous feature of eukaryotic cells...
  46. ncbi A method to purify bacteria-containing phagosomes from infected macrophages
    A Luhrmann
    Institut fur Zellbiologie, Universitat Bonn, 53121 Bonn, Germany
    Methods Cell Sci 22:329-41. 2000
    ..This method could also be used to study bacterial factors (proteins, RNA) produced while in phagosomes...
  47. pmc One-step affinity purification of the yeast ribosome and its associated proteins and mRNAs
    Toshifumi Inada
    Department of Molecular and Cell Biology, University of California at Berkeley, 94720, USA
    RNA 8:948-58. 2002
    ....
  48. ncbi The behavior of peroxisomes in vitro: mammalian peroxisomes are osmotically sensitive particles
    Vasily D Antonenkov
    Department of Biochemistry and Biocenter Oulu, University of Oulu, PO Box 3000, FIN 90014 Oulu, Finland
    Am J Physiol Cell Physiol 287:C1623-35. 2004
    ..g., polyethylene glycol 1500. A method was developed for the isolation of highly purified and nearly intact peroxisomes from rat liver by using polyethylene glycol 1500 as an osmoprotector...
  49. ncbi Purification of natural killer cell cytotoxic granules for assaying target cell apoptosis
    Joanne E Davis
    The Cancer Immunology Research Laboratory, The Peter MacCallum Cancer Institute, Victoria, Melbourne, Australia
    J Immunol Methods 276:59-68. 2003
    ....
  50. ncbi Subcellular fractionation and proteomics of nuclear envelopes
    Laurence Florens
    The Stowers Institute for Medical Research, Kansas City, MO, USA
    Methods Mol Biol 432:117-37. 2008
    ..To circumvent problems commonly associated with analyzing membrane proteins, the multidimensional protein identification technology (MudPIT) proteomics methodology is employed...
  51. ncbi Co-purification and direct interaction of Ras with caveolin, an integral membrane protein of caveolae microdomains. Detergent-free purification of caveolae microdomains
    K S Song
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142 1479, USA
    J Biol Chem 271:9690-7. 1996
    ....
  52. pmc Organellar proteomics reveals Golgi arginine dimethylation
    Christine C Wu
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 15:2907-19. 2004
    ..In this study, a stacked Golgi fraction was isolated by classical cell fractionation, and the protein complement (the Golgi proteome) was characterized using multidimensional protein ..
  53. ncbi Proteomics of regulated secretory organelles
    Yannick Brunner
    Biomedical Proteomics Research Group, University Medical Center, Geneva, Switzerland
    Mass Spectrom Rev 28:844-67. 2009
    ..The biological significance of regulated secretory organelles-proteomic analysis is discussed as well...
  54. ncbi Import of precursor proteins into isolated yeast mitochondria
    Nils Wiedemann
    Institut für Biochemie and Molekularbiologie, Universitat Freiburg, Germany
    Methods Mol Biol 313:373-83. 2006
    ..The process of mitochondrial protein import can be reconstituted in vitro using isolated mitochondria and in vitro synthesized precursor proteins...
  55. ncbi The nontransmembrane tyrosine phosphatase PTP-1B localizes to the endoplasmic reticulum via its 35 amino acid C-terminal sequence
    J V Frangioni
    Molecular Medicine Unit, Beth Israel Hospital, Boston, Massachusetts 02215
    Cell 68:545-60. 1992
    ..The C-terminal 35 amino acids of PTP-1B are both necessary and sufficient for targeting to the ER. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation...
  56. ncbi Accumulated metal speciation in earthworm populations with multigenerational exposure to metalliferous soils: cell fractionation and high-energy synchrotron analyses
    Jane Andre
    Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3US, UK
    Environ Sci Technol 43:6822-9. 2009
    ..These observations are highly significant in the challenging exercise of interpreting holistic biomarker data delivered by "omic" technologies...
  57. ncbi Breaking down the wall: fractionation of mycobacteria
    Mandana Rezwan
    Institut fur Medizinische Mikrobiologie, Gloriastrasse 32, Universität Zürich CH 8006 Zürich, Switzerland
    J Microbiol Methods 68:32-9. 2007
    ..The protocol of subcellular fractionation was then validated for pathogenic species by applying the method to Mycobacterium bovis BCG cells, an attenuated strain of the M. tuberculosis complex...
  58. ncbi The cytoplasmic tail of the vesicular acetylcholine transporter contains a synaptic vesicle targeting signal
    H Varoqui
    Neuroscience Center and Department of Pharmacology, Louisiana State University Medical Center, New Orleans, Louisiana 70112, USA
    J Biol Chem 273:9094-8. 1998
    ..Thus, structural information resides within the terminal cytoplasmic domain of VAChT, which specifically targets it to synaptic vesicles...
  59. pmc Evidence for coupling of membrane targeting and function of the signal recognition particle (SRP) receptor FtsY
    A A Herskovits
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    EMBO Rep 2:1040-6. 2001
    ..In contrast, separation by other means may interrupt proper synchronization of co-translational targeting and membrane assembly of NG. The co-translational interaction of FtsY with the membrane was confirmed by in vitro experiments...
  60. ncbi Identification of caveolae and caveolin in C6 glioma cells
    W I Silva
    Department of Physiology and Biophysics, University of Puerto Rico, San Juan
    Int J Dev Neurosci 17:705-14. 1999
    ..In addition, they demonstrate that cultured C6 glioma cells can be useful as a model system to study the role of CAV and caveolins in subcellular transport and signal transduction events in glial cells and the brain...
  61. ncbi The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast
    W E Gall
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235 1634, USA
    Curr Biol 10:1349-58. 2000
    ..Removal of the clathrin coat in vitro requires the uncoating ATPase Hsc70 and its DnaJ cofactor auxilin. To date, a requirement for auxilin and Hsc70 in clathrin function in vivo has not been demonstrated...
  62. ncbi The preparation of subcellular organelles from mouse liver in self-generated gradients of iodixanol
    J Graham
    Department of Biology, University of Essex, Colchester, United Kingdom
    Anal Biochem 220:367-73. 1994
    ..Because Iodixanol does not interfere significantly with marker enzyme activities, gradient fractions can be analyzed without removal of the gradient medium...
  63. ncbi Nuclear localization of phosphatidylinositol 4-kinase beta
    Petra de Graaf
    Molecular Cell Biology, Institute of Biomembranes, Universiteit Utrecht, The Netherlands
    J Cell Sci 115:1769-75. 2002
    ..These data demonstrate that PtdIns 4-kinase beta is present in the nuclei of NIH 3T3 fibroblasts, suggesting a specific function for this kinase in nuclear processes...
  64. ncbi In vitro culture of C. elegans somatic cells
    Kevin Strange
    Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, TN, USA
    Methods Mol Biol 351:265-73. 2006
    ..This chapter will provide a detailed description of the methods for isolating and culturing C. elegans embryo cells...
  65. ncbi Purification techniques of subcellular compartments for analytical and preparative purposes
    Laurence Aubry
    Laboratoire de Biochemie et Biophysique des Systèmes Intégrés, UMR 5092, Départment Réponse et Dynamique Cellulaires, CNRS CEA UJF, Grenoble, France
    Methods Mol Biol 346:171-85. 2006
    ..As analytical approaches, we propose procedures to label the plasma membrane and the endo-lysosomal compartments by biotinylation and to separate early and late compartments on a Percoll gradient...
  66. ncbi A cell fractionation approach for the quantitative analysis of subcellular drug disposition
    Muralikrishna Duvvuri
    Division of Drug Delivery and Disposition, School of Pharmacy, Kerr Hall C B 7360, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Pharm Res 21:26-32. 2004
    ..The purpose of this work was to develop and validate a method that can be used to quantify drugs associated with intracellular compartments...
  67. ncbi A novel subfractionation approach for mitochondrial proteins: a three-dimensional mitochondrial proteome map
    B J Hanson
    Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA
    Electrophoresis 22:950-9. 2001
    ..In addition, we show that this new approach provides functional information about protein complexes within the organelle that is not obtained with two-dimensional gel electrophoresis of whole mitochondria...
  68. ncbi Domain-specific disassembly and reassembly of nuclear membranes during mitosis
    B Buendia
    Departement de Biologie Cellulaire, Institut Jacques Monod, CNRS, Paris, France
    Exp Cell Res 230:133-44. 1997
    ..In these studies, protein LAP2/thymopoietin beta, another marker of the inner nuclear membrane, was segregating as lamin B receptor, suggesting that both proteins were contained in the same mitotic vesicles...
  69. ncbi Uncoupled packaging of targeting and cargo molecules during transport vesicle budding from the endoplasmic reticulum
    T Yeung
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA
    J Biol Chem 270:30567-70. 1995
    ..Using the same assay, we demonstrate that the packaging of targeting molecules into vesicles is not dependent on the lumenal chaperone, BiP (Kar2p)...
  70. ncbi Detergent resistance as a tool in membrane research
    Daniel Lingwood
    Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
    Nat Protoc 2:2159-65. 2007
    ..These protocols are completed in 1-2 d...
  71. pmc A cell-free assay allows reconstitution of Vps33p-dependent transport to the yeast vacuole/lysosome
    T Vida
    Department of Integrative Biology and Pharmacology, The University of Texas Health Science Center, Houston, Texas 77030, USA
    J Cell Biol 146:85-98. 1999
    ..This cell-free system has allowed the demonstration of reconstituted intercompartmental transport coupled to the function of a VPS gene product...
  72. ncbi Exploring the mechanisms of antigen processing by cell fractionation
    P Pierre
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520 8002, USA
    Curr Opin Immunol 10:145-53. 1998
    ..Data from recent cell fractionation studies, however, predominantly those using electrophoresis techniques, show that a population of class-II-..
  73. pmc Biochemical requirements for the targeting and fusion of ER-derived transport vesicles with purified yeast Golgi membranes
    V V Lupashin
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    J Cell Biol 132:277-89. 1996
    ..A model for the sequential involvement of these components in the targeting and fusion reaction is proposed...
  74. ncbi Direct binding of F-actin to ponticulin, an integral plasma membrane glycoprotein
    C P Chia
    Cell Biology Group, Worchester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545
    Cell Motil Cytoskeleton 18:164-79. 1991
    ..The 125I-labeled F-actin blot overlay assay also may enable us to identify F-actin-binding proteins in other cell types and should provide a convenient method for monitoring the purification of these proteins...
  75. ncbi Ultrastructural localization of bcl-2 protein
    P Monaghan
    Department of Cell Biology and Experimental Pathology, Institute of Cancer Research, Sutton, Surrey, UK
    J Histochem Cytochem 40:1819-25. 1992
    ....
  76. ncbi Association of cytochrome b translational activator proteins with the mitochondrial membrane: implications for cytochrome b expression in yeast
    U Michaelis
    Lehrstuhl Genetik, Universitat Munchen, FRG
    Mol Gen Genet 230:177-85. 1991
    ..A model is presented for translational activation of cytochrome b, which might also be applicable to translational regulation of other mitochondrial genes...
  77. ncbi Detailed analytical subcellular fractionation of non-pregnant porcine corpus luteum reveals peroxisomes of normal size and significant UDP-glucuronosyltransferase activity in the high-speed supernatant
    Malin Boström
    Department of Biochemistry and Biophysics, Arrhenius Laboratories for the Natural Sciences, Stockholm University, S 106 91 Stockholm, Sweden
    Chem Biol Interact 147:23-34. 2004
    ..In contrast, UGT sediments exclusively in the microsomal fraction upon subfractionation of the liver and ovary from rat...
  78. ncbi Targeting of biotinylated compounds to its target tissue using a low-density lipoprotein receptor-avidin fusion protein
    P Lehtolainen
    A I Virtanen Institute, University of Kuopio, Kuopio, Finland
    Gene Ther 10:2090-7. 2003
    ..These results suggest that local gene transfer of the fusion protein to target tissues may offer a novel tool for the delivery of biotinylated molecules in vitro and in vivo for therapeutic and imaging purposes...
  79. pmc Hepatitis B virus HBx protein localizes to mitochondria in primary rat hepatocytes and modulates mitochondrial membrane potential
    AMY J CLIPPINGER
    Graduate Program in Molecular and Cellular Biology and Genetics, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    J Virol 82:6798-811. 2008
    ....
  80. ncbi PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis
    Rong Rong
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Room 145, Whitehead Building, 615 Michael Street, Atlanta, Georgia 30322, USA
    Nat Neurosci 6:1153-61. 2003
    ..Our findings indicate that this complex mediates the well-known ability of agonists of mGluRI to prevent neuronal apoptosis...
  81. pmc Proapoptotic BH3-only proteins trigger membrane integration of prosurvival Bcl-w and neutralize its activity
    Julie Wilson-Annan
    Walter and Eliza Hall Institute of Medical Research, Victoria 3050, Australia
    J Cell Biol 162:877-87. 2003
    ..These results suggest that ligation of a proapoptotic BH3-only protein alters the conformation of Bcl-w, enhances membrane association, and neutralizes its survival function...
  82. ncbi Subcellular fractionation reveals proteins involved in insulin signaling
    Katie Cottingham
    J Proteome Res 5:739. 2006
  83. ncbi BAD and Bcl-2 regulation are early events linking neuronal endoplasmic reticulum stress to mitochondria-mediated apoptosis
    Wassim Elyaman
    Department of Anatomy, Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, Hong Kong
    Brain Res Mol Brain Res 109:233-8. 2002
    ..Altogether, our results indicate that neuronal ER stress is associated with an apoptotic cascade involving the mitochondria...
  84. pmc Subcellular localization of host and viral proteins associated with tobamovirus RNA replication
    Yuka Hagiwara
    Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    EMBO J 22:344-53. 2003
    ..These observations suggest that the formation of tobamoviral RNA replication complex occurs on TOM1-containing membranes and is facilitated by TOM2A...
  85. ncbi Cytosolic heat shock protein 60, hypoxia, and apoptosis
    S Gupta
    Baylor College of Medicine and the VA Medical Center, Houston, Tex, USA
    Circulation 106:2727-33. 2002
    ..We hypothesized that HSP60 would decrease with hypoxia/reoxygenation and that this would precipitate bax translocation to the mitochondria and release of cytochrome c...
  86. ncbi Detergent-resistant membrane subfractions containing proteins of plasma membrane, mitochondrial, and internal membrane origins
    Ronald L Mellgren
    Department of Physiology and Pharmacology, The University of Toledo College of Medicine, 3000 Arlington Avenue, Toledo, OH 43614 2598, USA
    J Biochem Biophys Methods 70:1029-36. 2008
    ..The subfractions described may prove useful in studying discrete protein populations associated with detergent-resistant membranes, and their potential interactions in cell signaling...
  87. ncbi Capillary electrophoretic separation of nuclei released from single cells
    Nilhan Gunasekera
    Department of Chemistry, University of Minnesota, 207 Pleasant Street S E, Minneapolis, MN 55455, USA
    Anal Chem 76:655-62. 2004
    ....
  88. pmc Phosphorylated BRCA1 is predominantly located in the nucleus and mitochondria
    Elisabeth D Coene
    N Goormaghtigh Institute of Pathology, University Hospital, B 9000 Gent, Belgium
    Mol Biol Cell 16:997-1010. 2005
    ..Furthermore, using cell fractionation, dephosphorylation, and enzyme protection experiments, we show that a 220-kDa phosphorylated isoform of ..
  89. pmc Subcellular localization of CIAPIN1
    Zhiming Hao
    State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi an 710032, Shaanxi Province, China
    J Histochem Cytochem 54:1437-44. 2006
    ..These findings suggest that CIAPIN1 may undergo a cytoplasm-nucleus-nucleolus translocation...
  90. pmc Soluble CPG15 expressed during early development rescues cortical progenitors from apoptosis
    Ulrich Putz
    The Picower Center for Learning and Memory, Departments of Brain and Cognitive Sciences and Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Neurosci 8:322-31. 2005
    ..CPG15 expressed during mammalian forebrain morphogenesis may help balance neuronal number by countering apoptosis in specific neuroblasts subpopulations, thus influencing final brain size and shape...
  91. ncbi New application of a subcellular fractionation method to kidney and testis for the determination of conjugated linoleic acid in selected cell organelles of healthy and cancerous human tissues
    Kristina Hoffmann
    Institute of Biochemistry and Food Chemistry, University of Hamburg, Grindelallee 117, Hamburg, Germany
    Anal Bioanal Chem 381:1138-44. 2005
    ..Because of these differences between tissues, no general statement on characteristic fatty acid profiles of single subcellular fractions is possible...
  92. ncbi Processing of the human protocadherin Fat1 and translocation of its cytoplasmic domain to the nucleus
    Thomas Magg
    University of Konstanz, Department of Biology, Box M648, D 78547 Konstanz, Germany
    Exp Cell Res 307:100-8. 2005
    ..In another step, the cleavage product was released to the cytosol and was also found in a low speed pellet fraction, in accordance with the nuclear localization of the cytoplasmic domain of hFat1...
  93. ncbi Specific and distinct determinants mediate membrane binding and lipid raft incorporation of HIV-1(SF2) Nef
    Simone I Giese
    Department of Virology, University of Heidelberg, 69120 Heidelberg, Germany
    Virology 355:175-91. 2006
    ....
  94. ncbi Characterization of N-glycosylation consensus sequences in the Kv3.1 channel
    Natasha L Brooks
    Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    FEBS J 273:3287-300. 2006
    ..1. We suggest that occupancy of the sites is critical for folding and maturation of the functional Kv3.1 at the cell surface...
  95. ncbi The membrane environment of endogenous cellular prion protein in primary rat cerebellar neurons
    Nicoletta Loberto
    Center of Excellence on Neurodegenerative Diseases, Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, Segrate, Italy
    J Neurochem 95:771-83. 2005
    ..Thus, prion protein plasma membrane environment in differentiated neurons resulted to be a complex entity, whose integrity requires a network of lipid-mediated non-covalent interactions...
  96. pmc Nuclear recycling of the pre-60S ribosomal subunit-associated factor Arx1 depends on Rei1 in Saccharomyces cerevisiae
    Nai Jung Hung
    Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, 1 University Station, A5000, The University of Texas at Austin, Austin, Texas 78712 0162, USA
    Mol Cell Biol 26:3718-27. 2006
    ..Furthermore, these results suggest that Rei1 is needed for release of Arx1 from nascent 60S subunits after export to the cytoplasm but not for the subsequent nuclear import of Arx1...
  97. pmc Fractionation of the epithelial apical junctional complex: reassessment of protein distributions in different substructures
    Roger Vogelmann
    Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305 5435, USA
    Mol Biol Cell 16:701-16. 2005
    ....
  98. ncbi Establishment of subcellular fractionation techniques to monitor the intracellular fate of polymer therapeutics I. Differential centrifugation fractionation B16F10 cells and use to study the intracellular fate of HPMA copolymer - doxorubicin
    F Philipp Seib
    Welsh School of Pharmacy, Centre for Polymer Therapeutics, Cardiff University, King Edward VII Avenue, Cardiff CF10 3XF, UK
    J Drug Target 14:375-90. 2006
    ..In conclusion, the B16F10 subcellular fractionation method developed here provides a useful tool to allow comparison of the intracellular trafficking of other polymer conjugates...
  99. ncbi Establishment of subcellular fractionation techniques to monitor the intracellular fate of polymer therapeutics II. Identification of endosomal and lysosomal compartments in HepG2 cells combining single-step subcellular fractionation with fluorescent imag
    Maria Manunta
    Welsh School of Pharmacy, Centre for Polymer Therapeutics, Cardiff University, King Edward VII Avenue, Cardiff, CF10 3XF, UK
    J Drug Target 15:37-50. 2007
    ....
  100. ncbi Hypertonic treatment inhibits radiation-induced nuclear translocation of the Ku proteins G22p1 (Ku70) and Xrcc5 (Ku80) in rat fibroblasts
    D Endoh
    Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069 8501, Japan Hokkaido Institute of Public Health, Sapporo 060 0819, Japan
    Radiat Res 155:320-7. 2001
    ..These results suggest that nuclear translocation of G22p1 and Xrcc5 is important for the fast repair process of PLD in rat cells...
  101. ncbi Isolation and purification of human placental plasma membranes from normal and pre-eclamptic pregnancies. a comparative study
    V Jimenez
    Programa de Fisiologia y Biofisica, Instituto de Ciencias Biomedicas ICBM, Facultad de Medicina, Universidad de Chile, Casilla 70005, Santiago 7, Chile
    Placenta 25:422-37. 2004
    ..We conclude that MVM and BM isolated from placentae of pre-eclamptic women are similar in enrichment and purity to those of healthy women, thus allowing their use in comparative electrophysiological studies...

Research Grants63

  1. Function of mRNA Editing in Trypanosomes
    Stephen L Hajduk; Fiscal Year: 2010
    ..based on coding sequence, RNA stability and the identification of predicted protein products by proteomic, cell fractionation and reverse genetics...
  2. Targeting Glucose Metabolism in Cancer
    Sucheta Telang; Fiscal Year: 2013
    ..Further, cell fractionation revealed that the PFKFB4 protein, and not PFKFB3, localized to the cytoplasm, the cellular compartment of ..
  3. P63/IGFBP3/BCL2 Control of Corneal Epithelial Homeostasis
    Danielle M Robertson; Fiscal Year: 2011
    ..constructs, and WB);(2) Establish membrane localization and subcellular trafficking of IGFBP3 (cell fractionation and WB, and biotinylated IGFPB3-streptavidin-conjugated quantum dots using time-lapse confocal microscopy);..
  4. The Role of PtdIns-5-P in Cell Function and Signaling
    LUCIA RAMEH-PLANT; Fiscal Year: 2012
    ..Using cell fractionation, we will determine the subcellular compartments where PI-5-P is present...
  5. Trafficking of the EGF receptor to the nucleus: Mechanisms and Effects
    Michael H Nathanson; Fiscal Year: 2013
    ..Intracellular movement of the receptor will be monitored by as well as by cell fractionation studies...
  6. Receptor Tyrosine Kinase Sorting and Transactivation in Endosomes
    MARK LINDSAY GRIMES; Fiscal Year: 2010
    ..ALK and TrkA are in the same or different endosomes in neuroblastoma cells using fluorescence microscopy and cell fractionation followed by immunoisolation of specific endosomes...
  7. The accessory Sec system of Gram-positive pathogens
    Paul M Sullam; Fiscal Year: 2013
    ..The subcellular localization of GspB and the Asps will be examined by cell fractionation and fluorescence microscopy...
  8. Estrogen-Induced Hippocampal Seizure Susceptibility
    Catherine S Woolley; Fiscal Year: 2013
    ..in brain slices from rats and mice, light and electron microscopic studies of synapses and vesicles, cell fractionation and immunoisolation of vesicles, and behavioral analyses of seizures to investigate how estradiol regulation ..
  9. Endothelial BK Channel Regulation by Caveolae Targeting
    Hon Chi Lee; Fiscal Year: 2010
    ..These will be assessed by cell fractionation, density gradient centrifugation, immunoprecipitation, immunofluorescence imaging, mutagenesis of hSIo and ..
  10. Nucleolin-mediated cell entry of DNA nanoparticles
    Pamela B Davis; Fiscal Year: 2010
    ..Fluorescence microscopy, cell fractionation, inhibitor studies, and siRNA analysis will be used to determine the trafficking through the cell...
  11. Interaction of RGS Protein with G beta subunit G beta 5.
    Vladlen Z Slepak; Fiscal Year: 2013
    ..The experiments will use advanced imaging methodology, cell fractionation and biochemical approaches to identify subcellular compartments where M3R and Gbeta5-RGS7 re-localize upon ..
  12. Mechanisms of estrogen action on bone marrow-derived stem stromal cells
    Charlotte Kuperwasser; Fiscal Year: 2011
    ..This will be investigated through the use of ERKO mouse models, bone marrow cell fractionation, and in vivo and in vitro functional assays to estrogen signaling...
  13. The role of CshA and CshB in selective mRNA protection in S. aureus
    AMBROSE LIN YAU CHEUNG; Fiscal Year: 2013
    ..b>Cell fractionation analysis, coupled with Northwestern blots with a labeled sarA mRNA probe, have enabled us to identify CshA, ..
  14. EBV EXPRESSION IN NASOPHARYNGEAL CARCINOMA
    NANCY JOAN RAAB-TRAUB; Fiscal Year: 2013
    ..induced by LMP1 and mutants of LMP1 that have distinct functional properties using immunofluorescence, cell fractionation, immunoprecipitation, 2-D fluorescent differential gel electrophoresis, and mass spectrometry. 3...
  15. Role of insulin-like signaling in the hookworm infective process
    John M Hawdon; Fiscal Year: 2010
    ..status and cellular localization of DAF-16 in non-activated and activated L3 will be determined by cell fractionation and Western blots, and the effect of ILS inhibitors on these parameters established...
  16. Al-2-Dependent Quorum Sensing in the Gram-Positive Bacterium Streptococcus pyogen
    Michael J Federle; Fiscal Year: 2010
    ..A biochemical approach complementary to that in Aim 1 will employ radiolabeled AI-2 and cell fractionation to identify the S. pyogenes Al-2 receptor...
  17. pH-sensing and activation of acid secretion by the V-ATPase
    Sylvie Breton; Fiscal Year: 2013
    ..V-ATPase assembly/disassembly will be examined using confocal microscopy, EM, co-IP and cell fractionation. We will "translate" our findings to A-ICs by using FACS isolated A-ICs and OMCDs perfused in vitro...
  18. Small molecule screen to enhance epithelial repair
    Barry R Stripp; Fiscal Year: 2012
    ..We have developed mouse lines and cell fractionation strategies that when coupled with a novel in vitro culture model allow us to quantify growth and ..
  19. Regulation of mRNA Partitioning to the Endoplasmic Reticulum
    Christopher V Nicchitta; Fiscal Year: 2010
    ..we will use mammalian tissue culture cell models and will emphasize established biochemical techniques of cell fractionation, cell biological analysis of reporter mRNA localization in situ and molecular biological analysis of the ..
  20. PENETRATION OF MACROMOLEULCES INTO CELLS
    H Ryser; Fiscal Year: 1999
    ..first 15-30 minutes) is followed by intracellular cleavage which, according to our preliminary data on cell fractionation, occurs in the Golgi apparatus...
  21. IMMUNOREGULATION OF GUT EPITHELIUM TO ESCAPE PARASITISM
    GILBERT CASTRO; Fiscal Year: 1991
    ..Electrophysiological and isotope tracer methodologies, cell fractionation procedures, lectin binding assays and enzymatic analyses will be used...
  22. LOCATION OF PORE SYSTEMS IN CAPILLARY WALLS
    George Palade; Fiscal Year: 2000
    ..Morphological and cell fractionation methods will be employed to study the entrance and exit of the tracer via transendothelial channels formed ..
  23. MECHANISMS OF MICROCYSTIN-LR HEPATOTOXICITY
    Val Beasley; Fiscal Year: 1993
    ..b>Cell fractionation studies using highly labelled 3H-dihydro-MCLR will be employed to assess the entry and sites of accumulation ..
  24. Role of ABCG5 and ABCG8 in Sterol Metabolism
    Helen Hobbs; Fiscal Year: 2007
    ..The subcellular location of ABCG5 and ABCG8 will be determined by microscopy and cell fractionation. The functional form of ABCG5 and ABCG8 will be determined by co-immunoprecipitation studies to identify the ..
  25. RESISTANCE--PATHOGENIC NAELERIA TO MO CYTOLYTIC FACTORS
    Francine Marciano Cabral; Fiscal Year: 1991
    ..b>Cell fractionation of amoebae will be performed using Percoll gradients...
  26. HEAT SHOCK PROTEINS IN E COLI CELL ENVELOPE
    MARY LUCKEY; Fiscal Year: 1993
    ..coli employing cell fractionation and electrophoretic analysis...
  27. ROLE OF PHOSPHATIDIC ACID IN GROWTH FACTOR SIGNALING
    Guillermo Romero; Fiscal Year: 2003
    ..induced by insulin and PDGF, describing complete time courses using both real time live cell imaging and cell fractionation approaches...
  28. PROTEINS OF THE HEPATOMA CELL PLASMA MEMBRANE
    DARRELL DOYLE; Fiscal Year: 1991
    ..of the proposed research is to identify by a variety of methods, including external labeling, immunological, cell fractionation and cytochemical the proteins and the glycoproteins that are externally oriented in the plasma membrane of ..
  29. Efficacy and Mechanism of Novel Androgen Receptor Inhibitors
    Marc I Diamond; Fiscal Year: 2010
    ..We will test for effects on AR nuclear localization via cell fractionation and western blot...
  30. Biogenesis and Function of Bacterial Amyloid fibers
    Matthew Chapman; Fiscal Year: 2005
    ....
  31. Alpha- & Gamma-Synuclein Modulation of the NET After Antidepressant Treatment of
    Alexis Jeannotte; Fiscal Year: 2007
    ..We will measure expression levels and colocalization of these proteins before and after DMI treatment, by cell fractionation, Western blots and immunohistochemistry...
  32. EFFECTS OF HYPERTHERMIA ON IMMUNE FUNCTIONS IN VITRO
    EDDIE LAMON; Fiscal Year: 1992
    ..2 - 7.8. Cell fractionation studies will be done to evaluate the differential development in heatshock proteins (HSP) in cells that ..
  33. PROTEIN KINASE C AND SMOOTH MUSCLE CONTRACTION
    HOWARD RASMUSSEN; Fiscal Year: 1993
    ..The effect of agonists such as histamine and norepinephrine on the cellular distribution of PKC using both cell fractionation and immunocytochemistry will also be carried out...
  34. SUBCELLULAR EFFECTS OF METABOLIC HORMONES ON RAT LIVER
    ROBERT CARDELL; Fiscal Year: 1991
    ..with results from the autoradiographic experiments we will isolate cellular organelles and glycogen by cell fractionation procedures, quantitate the amount of label in these cellular components after pulse labels of either 3H-..
  35. MOLECULAR BIOLOGY OF CILIATED PROTOZOA
    CHARLES ALLIS; Fiscal Year: 1993
    ..for studying function in cell biology, ciliated protozoa offer several advantages over yeast such as ease in cell fractionation and cytological analyses. Moreover, some features of most eukaryotic cells are not found in S...
  36. AN INTEGRATED STUDY OF THE OVIDUCT BASAL BODY AND CILIUM
    Richard Anderson; Fiscal Year: 1980
    ..1) To utilize both in vivo and in vitro techniques, as well as cell fractionation techniques, to investigate the biochemical properties of basal bodies and cilia isolated from adult chicken ..
  37. Regulation of Sporulation in Myxococcus xanthus
    David Zusman; Fiscal Year: 2005
    ..We plan to follow the localization of EspA and EspB during development by cell fractionation studies, fluorescence microscopy, and construction and tracking of GFP fusions...
  38. REGULATION OF HORMONE-SENSITIVE EFFECTOR SYSTEMS
    Craig C Malbon; Fiscal Year: 2010
    ..including 2-photon,/cs, and TIRF microscopy), energy transfer measurements In live cells (via BRET), and cell fractionation coupled with TAP-tagged Dvl followed by proteomics...
  39. REGULATION OF RECEPTOR MEDIATED ENDOCYTOSIS--HUMAN CELL
    Robert Fallon; Fiscal Year: 1990
    ..These studies involve binding of radioactively labelled ligand, cell fractionation and immunological isolation of internalized receptor, and colloidal gold immunocytoelectron microscopy...
  40. Regulation of heme uptake in intestinal epithelial cells
    Aliye Uc; Fiscal Year: 2007
    ..the vesicular transport mechanisms involved in intestinal heme uptake by using electron microscopy and cell fractionation techniques and colocalization with proteins that are trasported via clathrin or caveolae/lipid raft ..
  41. Cyclin Dependent Kinase 6 in Cardiac Development
    Hal Skopicki; Fiscal Year: 2006
    ..Using cell fractionation, indirect multi-labeling immunofluorescence and FACs analysis, we have described and quantified the temporal ..
  42. DEVELOPMENTAL REGULATION OF GLUTAMATE RECEPTOR FUNCTION
    Martha Constantine Paton; Fiscal Year: 2001
    ..use RNase protection assays, competitive PCR, immunoblotting, immunocytochemistry, immunoprecipitation and cell fractionation procedures...
  43. MOLECULAR PHYSIOLOGY OF THE SODIUM PUMP
    Jack Kaplan; Fiscal Year: 2006
    ..a protein of about 300 amino acids and a single membrane crossing) separately and together and using cell fractionation and metabolic labelling strategies investigate the factors which control their assembly in the endoplasmic ..
  44. REGULATION OF IMMUNITY AT THE MOLECULAR LEVEL
    Howard Dintzis; Fiscal Year: 1980
    ..b>Cell fractionation and culture methods to identify the specific cell types participating in the response and controlling its ..
  45. The Adenovirus Receptor CAR in Epithelial Malignancies
    WOLFGANG KORN; Fiscal Year: 2007
    ..of CAR in response to Ras/MAPK and TGF-beta signaling, techniques for labeling surface proteins and cell fractionation will be utilized...
  46. Rare Cell Isolation for Morphological & Molecular Study
    Jody Vykoukal; Fiscal Year: 2004
    ..To this end we propose a versatile, dielectrophoresis-based cell fractionation and isolation platform that is an outgrowth of NCI IMAT funded studies currently underway at M.D...
  47. Novel Approaches to Niemann-Pick Type C Disease
    ROBERT MAUE; Fiscal Year: 2004
    ..We will evaluate the morphological responses to other growth factors, and use cell fractionation and gradient centrifugation to determine if the localization of TrkB and other growth factor receptors to ..
  48. The Role of Pka Anchoring in Cell Differentiation
    SHERELL BYRD; Fiscal Year: 2005
    ..differentiation process, we will examine changes in AKAP expression during L6 myoblast differentiation using cell fractionation, and identify those differentially expressed AKAPs through traditional western blotting methods...
  49. PHOTORECEPTOR MEMBRANES; CYCLING AND SORTING
    Darcy Kelley; Fiscal Year: 1992
    ..microscopic cytochemistry, immunocytochemistry and autoradiography with supportive fluorescence microscopic, cell fractionation, physiological and biochemical determinations...
  50. BIOSYNTHESIS OF VON WILLEBRAND FACTOR BY ENDOTHELIUM
    Bruce Ewenstein; Fiscal Year: 1990
    ..VWD-derived cultured vascular endothelium using the techniques of molecular biology, protein biochemistry, cell fractionation, and ultrastructural analysis...
  51. NOVEL EFFECTORS OF GRB2 IN HUMAN GLIAL TUMORS
    Albert Wong; Fiscal Year: 2009
    ..stimulation by :1) studying the localization of Gab1/SHP2 interaction after oxidative stress via FRET and cell fractionation;2) the phosphorylation sites of Gab1 after H2O2 addition as revealed by MS-MALDI;and 3) how JNK is ..
  52. Antigen Presentation Pathway by CD1 Molecules
    Mitchell Kronenberg; Fiscal Year: 2006
    ..second aim, we will express mutant mCD1d molecules with restricted intracellular trafficking, and carry out cell fractionation studies to determine where processing and mCD1 antigen binding occur...
  53. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 2005
    ..Genetic and cell fractionation studies are proposed to discover the genes that govern sorting of cell surface precursors in the early and ..
  54. ULTRASTRUCTURE OF LEUKOCYTES AND PLATELETS
    Dorothea Zucker Franklin; Fiscal Year: 1992
    ..microscopy, immunohistochemistry on the light microscopic and ultrastructural level, freeze-fracture, cell fractionation and tissue culture...
  55. HIV1 ASSEMBLY MECHANISM AND INHIBITION
    Lee Ratner; Fiscal Year: 2002
    ..In each case, they will examine the effects on membrane interactions and the structure and expression of extracellular virions, as well as identify the domains in Pr55gag that are critical for regulation by Vif or Vpu. ..
  56. GROWTH CONTROL IN AGING FIBROBLASTS
    Eugenia Wang; Fiscal Year: 1992
    ..Biochemcial studies will be pursued by cell fractionation followed by 1- and 2-D gel analysis, coupled with immunoprecipitation and immunoblotting assays...
  57. THE GOLGI PHASE OF LIPOPROTEIN METABOLISM IN THE LIVER
    Kathryn Howell; Fiscal Year: 1980
    ..The secretory route they travel has been followed by morphology and cell fractionation studies...
  58. Osteoprogenitor Subpopulations within Adipose-derived Stroma
    Deepak Gupta; Fiscal Year: 2009
    ..The first Specific Aim focuses on performing cell fractionation experiments on primary cultures of cells derived from inguinal fat pads of adult mice...
  59. Regulation of protein translation by heat shock proteins
    ROBERT SEISER; Fiscal Year: 2004
    ..First, cell fractionation and protein analysis will be used to determine the distribution of Sis1 protein with respect to ribosomes ..
  60. Glomerular Capillaries-- Normal and Pathologic
    Marilyn Farquhar; Fiscal Year: 2008
    ..abstract_text> ..
  61. High Resolution Digital Camera for TEM.
    Bridget Wilson; Fiscal Year: 2006
    ..The new camera will ensure the continued productivity of NIH-funded electron microscopy-based research programs in cellular and molecular biology and neurosciences at the University of New Mexico. [unreadable] [unreadable]..
  62. B-CLL Proteins Quantified by Mass Spectrometry
    DAVID BARNIDGE; Fiscal Year: 2004
    ..As part of this proposal the candidate will take courses offered in the Mayo Graduate School on Techniques in Molecular Biology, Human Cytogenetics, and Immunology. ..
  63. HORMONE SECRETION AND PROCESSING IN PITUITARY CELLS
    Marilyn Gist Farquhar; Fiscal Year: 2010
    ..These studies can be expected to considerably expand knowledge of the role of G proteins in regulated secretion of hormones and in endocytosis and signaling of hormones by GPCR. ..