organ culture techniques


Summary: A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)

Top Publications

  1. ncbi WT-1 is required for early kidney development
    J A Kreidberg
    Whitehead Institute for Biomedical Research Cambridge, Massachusetts 02142
    Cell 74:679-91. 1993
  2. ncbi Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases
    Stephen C Noctor
    Department of Neurology, Columbia University College of Physicians and Surgeons, 630 W 168th Street, New York, New York 10032, USA
    Nat Neurosci 7:136-44. 2004
  3. pmc LRRTM2 interacts with Neurexin1 and regulates excitatory synapse formation
    Joris De Wit
    Neurobiology Section, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Neuron 64:799-806. 2009
  4. ncbi Gain modulation from background synaptic input
    Frances S Chance
    Center for Neural Science, New York University, New York, NY 10003, USA
    Neuron 35:773-82. 2002
  5. ncbi NGF signaling in sensory neurons: evidence that early endosomes carry NGF retrograde signals
    Jean Dominique Delcroix
    Department of Neurology and Neurological Sciences, Stanford University, Stanford 94305, USA
    Neuron 39:69-84. 2003
  6. ncbi SK channels regulate excitatory synaptic transmission and plasticity in the lateral amygdala
    E S Louise Faber
    The Queensland Brain Institute, University of Queensland, Brisbane, Queensland 4072, Australia
    Nat Neurosci 8:635-41. 2005
  7. ncbi Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair
    Jens Noraberg
    Anatomy and Neurobiology, Institute of Medical Biology, University of Southern Denmark, Odense, Denmark
    Curr Drug Targets CNS Neurol Disord 4:435-52. 2005
  8. doi Muscarinic receptor activation disrupts hippocampal sharp wave-ripples
    Hiroaki Norimoto
    Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113 0033, Japan
    Brain Res 1461:1-9. 2012
  9. doi Comparison of bevacizumab, ranibizumab, and pegaptanib in vitro: efficiency and possible additional pathways
    Alexa Klettner
    Department of Ophthalmology, University of Kiel, Kiel, Germany
    Invest Ophthalmol Vis Sci 49:4523-7. 2008
  10. pmc Cisplatin ototoxicity in rat cochlear organotypic cultures
    Dalian Ding
    Center for Hearing and Deafness, State University of New York at Buffalo, 137 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA
    Hear Res 282:196-203. 2011

Detail Information

Publications420 found, 100 shown here

  1. ncbi WT-1 is required for early kidney development
    J A Kreidberg
    Whitehead Institute for Biomedical Research Cambridge, Massachusetts 02142
    Cell 74:679-91. 1993
    ..In addition, the mutation caused abnormal development of the mesothelium, heart, and lungs. Our results establish a crucial role for WT-1 in early urogenital development...
  2. ncbi Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases
    Stephen C Noctor
    Department of Neurology, Columbia University College of Physicians and Surgeons, 630 W 168th Street, New York, New York 10032, USA
    Nat Neurosci 7:136-44. 2004
    ..These findings provide a comprehensive and new view of the dynamics of cortical neurogenesis and migration...
  3. pmc LRRTM2 interacts with Neurexin1 and regulates excitatory synapse formation
    Joris De Wit
    Neurobiology Section, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Neuron 64:799-806. 2009
    ..These observations indicate that an LRRTM2-Neurexin1 interaction plays a critical role in regulating excitatory synapse development...
  4. ncbi Gain modulation from background synaptic input
    Frances S Chance
    Center for Neural Science, New York University, New York, NY 10003, USA
    Neuron 35:773-82. 2002
    ..These results suggest that, within active cortical circuits, the overall level of synaptic input to a neuron acts as a gain control signal that modulates responsiveness to excitatory drive...
  5. ncbi NGF signaling in sensory neurons: evidence that early endosomes carry NGF retrograde signals
    Jean Dominique Delcroix
    Department of Neurology and Neurological Sciences, Stanford University, Stanford 94305, USA
    Neuron 39:69-84. 2003
    ..Our results are evidence that signaling endosomes, with the characteristics of early endosomes, convey NGF signals from the target of nociceptive neurons to their cell bodies...
  6. ncbi SK channels regulate excitatory synaptic transmission and plasticity in the lateral amygdala
    E S Louise Faber
    The Queensland Brain Institute, University of Queensland, Brisbane, Queensland 4072, Australia
    Nat Neurosci 8:635-41. 2005
    ..Calcium influx through NMDARs activates SK channels and shunts the resultant excitatory postsynaptic potential. These results demonstrate a new role for SK channels as postsynaptic regulators of synaptic efficacy...
  7. ncbi Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair
    Jens Noraberg
    Anatomy and Neurobiology, Institute of Medical Biology, University of Southern Denmark, Odense, Denmark
    Curr Drug Targets CNS Neurol Disord 4:435-52. 2005
  8. doi Muscarinic receptor activation disrupts hippocampal sharp wave-ripples
    Hiroaki Norimoto
    Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113 0033, Japan
    Brain Res 1461:1-9. 2012
    ..Thus, cholinergic input is likely to contribute to a neuromodulatory switch of hippocampal network states, as proposed in the "two-stage" model of learning processes...
  9. doi Comparison of bevacizumab, ranibizumab, and pegaptanib in vitro: efficiency and possible additional pathways
    Alexa Klettner
    Department of Ophthalmology, University of Kiel, Kiel, Germany
    Invest Ophthalmol Vis Sci 49:4523-7. 2008
    ..In this study, the authors compare these VEGF inhibitors directly regarding their efficiency to neutralize VEGF in a quantifiable in vitro system...
  10. pmc Cisplatin ototoxicity in rat cochlear organotypic cultures
    Dalian Ding
    Center for Hearing and Deafness, State University of New York at Buffalo, 137 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA
    Hear Res 282:196-203. 2011
  11. doi Postnatal developmental profile of neurons and glia in motor nuclei of the brainstem and spinal cord, and its comparison with organotypic slice cultures
    Alessandra Cifra
    Neurobiology Sector and IIT Unit, International School for Advanced Studies SISSA, Trieste, Italy
    Dev Neurobiol 72:1140-60. 2012
    ..The present report indicates a comparable degree of neuronal and glial maturation in brainstem and spinal motor nuclei, and that this condition is also observed in 3-week-old organotypic cultures...
  12. doi Determinants of spontaneous activity in networks of cultured hippocampus
    Eyal Cohen
    Department of Neurobiology, The Weizmann Institute, Rehovot, Israel
    Brain Res 1235:21-30. 2008
    ..We conclude that networks of neurons in culture can produce spontaneous synchronized activity and serve as a viable model system for the analysis of the rules that govern network activity in the brain...
  13. doi Thyroid-stimulating hormone, a novel, locally produced modulator of human epidermal functions, is regulated by thyrotropin-releasing hormone and thyroid hormones
    Eniko Bodo
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    Endocrinology 151:1633-42. 2010
    ..Intraepidermal TSH expression appears to be regulated by the classical endocrine controls that determine the systemic HPT axis...
  14. doi Enhanced neurogenesis in organotypic cultures of rat hippocampus after transient subfield-selective excitotoxic insult induced by domoic acid
    A Perez-Gomez
    Department of Biomedical Sciences, University of Prince Edward Island, Charlottetown, PEI, Canada
    Neuroscience 208:97-108. 2012
  15. ncbi Porcine vagina ex vivo as a model for studying permeability and pathogenesis in mucosa
    Christopher A Squier
    Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 54222, USA
    J Pharm Sci 97:9-21. 2008
  16. pmc The actions of BDNF on dendritic spine density and morphology in organotypic slice cultures depend on the presence of serum in culture media
    Christopher A Chapleau
    Department of Neurobiology, Civitan International Research Center, McKnight Brain Institute, The University of Alabama at Birmingham, Birmingham, AL 35294 2182, USA
    J Neurosci Methods 169:182-90. 2008
  17. pmc The MAPK(ERK-1,2) pathway integrates distinct and antagonistic signals from TGFalpha and FGF7 in morphogenesis of mouse mammary epithelium
    Jimmie E Fata
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Dev Biol 306:193-207. 2007
    ..Our findings indicate that expression of morphogenetic effectors, proliferation, and cell-type decisions during mammary organoid morphogenesis are intimately dependent on the duration of activation of MAPK(ERK1,2) activation...
  18. pmc Memory CD4(+) T cells are the earliest detectable human immunodeficiency virus type 1 (HIV-1)-infected cells in the female genital mucosal tissue during HIV-1 transmission in an organ culture system
    Phalguni Gupta
    Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Virol 76:9868-76. 2002
  19. doi Human hair follicles contain two forms of ATP-sensitive potassium channels, only one of which is sensitive to minoxidil
    Katie Shorter
    Department of Biomedical Sciences, University of Bradford, Bradford, BD7 1DP, UK
    FASEB J 22:1725-36. 2008
  20. ncbi TNF alpha potentiates glutamate neurotoxicity by inhibiting glutamate uptake in organotypic brain slice cultures: neuroprotection by NF kappa B inhibition
    Jian Y Zou
    Bowles Center for Alcohol Studies, CB 7178 Thurston Bowles Building, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7178, USA
    Brain Res 1034:11-24. 2005
    ..TNF alpha potentiation of glutamate neurotoxicity through the blockade of glutamate transporter activity may represent an important mechanism of neurodegeneration associated with neuroinflammation...
  21. ncbi Catenary cultures of embryonic gastrointestinal tract support organ morphogenesis, motility, neural crest cell migration, and cell differentiation
    C J Hearn
    The Murdoch Institute, Parkville, Victoria, Australia
    Dev Dyn 214:239-47. 1999
    ..Thus, cell types representative of all three germ layer contributions developed, and in addition, the gut, being mainly free, was able to elongate and bend (unlike on solid support cultures), while retaining its rostrocaudal identity...
  22. ncbi Lack of NMDA receptor subtype selectivity for hippocampal long-term potentiation
    Sven Berberich
    Department of Molecular Neurobiology, Max Planck Institute for Medical Research, D 69120 Heidelberg, Germany
    J Neurosci 25:6907-10. 2005
  23. doi Metformin decreases the adipokine vaspin in overweight women with polycystic ovary syndrome concomitant with improvement in insulin sensitivity and a decrease in insulin resistance
    Bee K Tan
    Endocrinology and Metabolism Group, Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Coventry, UK
    Diabetes 57:1501-7. 2008
    ..Ex vivo regulation of adipose tissue vaspin and the effects of metformin treatment on circulating vaspin levels in PCOS subjects were also studied...
  24. pmc HIV binding, penetration, and primary infection in human cervicovaginal tissue
    Diane Maher
    Department of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware Street Southeast, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 102:11504-9. 2005
    ..Collectively, these results highlight a continuum of complex interactions that occurs when natural sources of HIV infectivity are deposited onto mucosal surfaces in the female reproductive tract...
  25. doi ERK- and Akt-dependent neuroprotection by erythropoietin (EPO) against glyoxal-AGEs via modulation of Bcl-xL, Bax, and BAD
    Jianfeng Shen
    Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China
    Invest Ophthalmol Vis Sci 51:35-46. 2010
    ..To characterize the neuroprotective mechanisms of erythropoietin (EPO) against the stress of glyoxal-advanced glycation end products (AGEs) in retinal neuronal cells...
  26. ncbi Precision-cut organ slices to investigate target organ injury
    Alison E M Vickers
    Allergan, Inc, RD2 2A, 2525 Dupont Drive, Irvine CA 92623, USA
    Expert Opin Drug Metab Toxicol 1:687-99. 2005
  27. doi 2-Arachidonoylglycerol elicits neuroprotective effects on excitotoxically lesioned dentate gyrus granule cells via abnormal-cannabidiol-sensitive receptors on microglial cells
    Susanne Kreutz
    Dr Senckenbergische Anatomie, Institut für Anatomie II, J W Goethe Universitat, Frankfurt am Main, Germany
    Glia 57:286-94. 2009
    ..We therefore propose that the endocannabinoid 2-AG exerts its neuroprotective effects via activation of abn-CBD-sensitive receptors on microglial cells...
  28. doi Prolactin--a novel neuroendocrine regulator of human keratin expression in situ
    Yuval Ramot
    Department of Dermatology, University of Lubeck, Lubeck, Germany
    FASEB J 24:1768-79. 2010
    ..Therefore, our study identifies PRL as a major, clinically relevant, novel neuroendocrine regulator of both human keratin expression and human epithelial stem cell biology in situ...
  29. ncbi Up-regulation of alpha1A-adrenoceptors in rat mesenteric artery involves intracellular signal pathways
    Yong xiao Cao
    Division of Experimental Vascular Research, Institution of Medicine, Lund University, Sweden
    Basic Clin Pharmacol Toxicol 98:61-7. 2006
    ..SB386023 did not affect alpha2-adrenoceptor mRNA level. Our results suggest that the up-regulation of alpha1A-adrenoceptors involves transcription and intracellular signal transduction via the protein kinase C and the ERK 1/2 pathways...
  30. pmc HCN hyperpolarization-activated cation channels inhibit EPSPs by interactions with M-type K(+) channels
    Meena S George
    Department of Neuroscience, Columbia University, New York, New York, USA
    Nat Neurosci 12:577-84. 2009
    ..In this manner, I(h) can enhance spike firing in response to an EPSP when spike threshold is low and can inhibit firing when spike threshold is high...
  31. ncbi Activation of class I metabotropic glutamate receptors limits dendritic growth of Purkinje cells in organotypic slice cultures
    Alexandra Sirzen-Zelenskaya
    Anatomisches Institut, Universitat Basel, Pestalozzistrasse 20, CH 4056 Basel, Switzerland
    Eur J Neurosci 24:2978-86. 2006
    ..This developmental mechanism could protect Purkinje cells from excitotoxic death through excessive release of glutamate from an overload of parallel fiber contacts...
  32. ncbi Real-time analysis of ureteric bud branching morphogenesis in vitro
    Tomoko Watanabe
    Department of Genetics and Development, Columbia University, New York, NY 10032, USA
    Dev Biol 271:98-108. 2004
  33. doi Synaptic mGluR activation drives plasticity of calcium-permeable AMPA receptors
    Leah Kelly
    Department of Neuroscience, Physiology and Pharmacology, University College London, UK
    Nat Neurosci 12:593-601. 2009
    ..Regulation by both excitatory and inhibitory inputs provides an unexpected mechanism that determines the potential of these synapses to show dynamic changes in AMPAR Ca(2+) permeability...
  34. ncbi Palatal shelf movement during palatogenesis: a fate map of the fetal mouse palate cultured in vitro
    Ming Jen Chou
    Department of Anatomy and Developmental Biology, Kyoto University Graduate School of Medicine, 606 8501 Kyoto, Japan
    Anat Embryol (Berl) 208:19-25. 2004
  35. ncbi Bmp4 and Fgf10 play opposing roles during lung bud morphogenesis
    M Weaver
    Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt Medical Center, Nashville, TN, USA
    Development 127:2695-704. 2000
    ..By contrast, the Bmp-binding protein Noggin enhances Fgf-induced morphogenesis. Based on these and other results, we propose a model for the combinatorial roles of Fgf10 and Bmp4 in branching morphogenesis of the lung...
  36. pmc Adenosine A1 receptors and microglial cells mediate CX3CL1-induced protection of hippocampal neurons against Glu-induced death
    Clotilde Lauro
    Istituto Pasteur, Fondazione Cenci Bolognetti, Rome, Italy
    Neuropsychopharmacology 35:1550-9. 2010
  37. ncbi Retinoid-related orphan receptor alpha controls the early steps of Purkinje cell dendritic differentiation
    Fatiha Boukhtouche
    Universite Pierre et Marie Curie PARIS6, Unité Mixte de Recherche 7102 Neurobiologie des Processus Adaptatifs, Centre National de la Recherche Scientifique, UMR 7102 NPA, Paris, F 75005, France
    J Neurosci 26:1531-8. 2006
    ..However, it does not seem to participate to the last stage of dendritic growth. This study identifies RORalpha as a nuclear receptor crucial for the control of dendritic remodeling during development...
  38. ncbi NMDA receptors and the differential ischemic vulnerability of hippocampal neurons
    Christine E Gee
    Brain Research Institute, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Eur J Neurosci 23:2595-603. 2006
    ..Therapeutic modulation of tyrosine phosphorylation may thus prove beneficial in mitigating ischemia-induced neuronal death in vulnerable brain areas...
  39. doi Hypothermia-induced neurite outgrowth is mediated by tumor necrosis factor-alpha
    Katharina R L Schmitt
    Clinic for Congenital Heart Disease and Pediatric Cardiology, Deutsches Herzzentrum, Berlin, Germany
    Brain Pathol 20:771-9. 2010
    ..These data suggest that hypothermia not only exerts protective effects in the CNS but may also support neurite outgrowth as a potential mechanism of regeneration...
  40. ncbi Endogenous estrogens inhibit mouse fetal Leydig cell development via estrogen receptor alpha
    Geraldine Delbes
    Unité de Gamétogenèse et Génotoxicité, Institut National de la Sante et de la Recherche Medicale Unite 566, Commissariat a l Energie Atomique, Universite Paris 7, Denis Diderot, Fontenay aux Roses, France
    Endocrinology 146:2454-61. 2005
    ..Thus, this study shows that endogenous estrogens physiologically inhibit steroidogenesis via ER alpha by acting directly on the testis early in fetal and neonatal development...
  41. ncbi Reduced thymocyte development in sonic hedgehog knockout embryos
    Divya K Shah
    Department of Biological Sciences, Imperial College London, London, United Kingdom
    J Immunol 172:2296-306. 2004
  42. pmc The functional nature of synaptic circuitry is altered in area CA3 of the hippocampus in a mouse model of Down's syndrome
    Jesse E Hanson
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305 5345, USA
    J Physiol 579:53-67. 2007
  43. doi Gamma oscillations and spontaneous network activity in the hippocampus are highly sensitive to decreases in pO2 and concomitant changes in mitochondrial redox state
    Christine Huchzermeyer
    Institute for Neurophysiology, Charite Universitatsmedizin Berlin, 10117 Berlin, Germany
    J Neurosci 28:1153-62. 2008
    ..Our study highlights the importance of a functional understanding of mitochondria and their implications on activities of individual neurons and neuronal networks...
  44. doi Cell death and proliferation in acute slices and organotypic cultures of mammalian CNS
    Laura Lossi
    Dipartimento di Morfofisiologia Veterinaria, University of Turin, Turin, Italy
    Prog Neurobiol 88:221-45. 2009
    ..e. the cortical, hippocampal, cerebellar and retinal slices...
  45. pmc Initial events in establishing vaginal entry and infection by human immunodeficiency virus type-1
    Florian Hladik
    Program in Infectious Diseases, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Immunity 26:257-70. 2007
    ..Our findings shed light on the very earliest steps of mucosal HIV infection in vivo and may guide the design of effective strategies to block local transmission and prevent HIV-1 spread...
  46. ncbi Morphological and electrophysiological properties of GABAergic and non-GABAergic cells in the deep cerebellar nuclei
    Marylka Uusisaari
    Laboratory for Neuronal Circuit Dynamics, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Neurophysiol 97:901-11. 2007
    ..We conclude that GABAergic DCN neurons are specialized to convey phasic spike rate information, whereas tonic spike rate is more faithfully relayed by the large non-GABAergic cells...
  47. ncbi Dynamics of microglial activation: a confocal time-lapse analysis in hippocampal slices
    N Stence
    Department of Biological Sciences, 355 Biology Building, University of Iowa, Iowa City, IA 52242, USA
    Glia 33:256-66. 2001
    ..More generally, our time-lapse observations identify changes in the dynamic behavior of activating microglia and thereby help define distinct temporal and functional stages of activation for further investigation...
  48. ncbi An in vitro organ culturing system for intervertebral disc explants with vertebral endplates: a feasibility study with ovine caudal discs
    Benjamin Gantenbein
    AO Research Institute, Davos, Switzerland
    Spine (Phila Pa 1976) 31:2665-73. 2006
    ..Whole ovine caudal intervertebral discs with vertebral endplates were cultured under uniaxial diurnal loading for 7 days...
  49. ncbi Neuron and glia generating progenitors of the mammalian enteric nervous system isolated from foetal and postnatal gut cultures
    Nadege Bondurand
    Division of Molecular Neurobiology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Development 130:6387-400. 2003
  50. ncbi Time-lapse analysis of living mouse germ cell migration
    K A Molyneaux
    Division of Developmental Biology, Children's Hospital Research Foundation, Cincinnati, Ohio 45229, USA
    Dev Biol 240:488-98. 2001
    ..5 migrate into the genital ridges. Finally, at E11.5, PGCs are slowing and the direction of movement is dependent on the sex of the embryo. This allows, for the first time, a formal description of the events of PGC migration in the mouse...
  51. ncbi Organotypic cultures as tool to test long-term effects of chemicals on the nervous system
    F Pena
    Departamento de Farmacobiologia, Mexico D F, Mexico
    Curr Med Chem 17:987-1001. 2010
    ..Overall, this review will provide elements to support the use of organotypic cultures as a very reliable model to explore long-term neuropharmacological studies in vitro...
  52. pmc Human diabetic corneas preserve wound healing, basement membrane, integrin and MMP-10 differences from normal corneas in organ culture
    Andrea Kabosova
    Ophthalmology Research Laboratories, Cedars Sinai Medical Center, Burns and Allen Research Institute, Los Angeles, CA 90048, USA
    Exp Eye Res 77:211-7. 2003
    ..The established corneal organ culture provides an adequate system for elucidating mechanisms of epithelial alterations in human DR corneas...
  53. ncbi Synchrony-dependent propagation of firing rate in iteratively constructed networks in vitro
    Alex D Reyes
    Center for Neural Science, New York University, 4 Washington Place, New York, New York 10003, USA
    Nat Neurosci 6:593-9. 2003
    ..The firing rate was represented by a classical rate code in the initial layers, but switched to a synchrony-based code in the deeper layers...
  54. doi Developmental profile and properties of sulforhodamine 101--Labeled glial cells in acute brain slices of rat hippocampus
    Karl Wolfgang Kafitz
    Institut fur Neurobiologie, Heinrich Heine Universitat Dusseldorf, Universitatsstrasse 1, D 40225 Dusseldorf, Germany
    J Neurosci Methods 169:84-92. 2008
    ..Owing to its reliability and uncomplicated handling, we expect that this technique will be helpful in future investigations studying astrocytes in the developing brain...
  55. ncbi Human hair follicles display a functional equivalent of the hypothalamic-pituitary-adrenal axis and synthesize cortisol
    Natsuho Ito
    Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan
    FASEB J 19:1332-4. 2005
  56. ncbi Cellular and molecular control of dendritic growth and development of cerebellar Purkinje cells
    Josef P Kapfhammer
    Universitat Basel, Anatomisches Institut, Pestalozzistrasse 20, CH 4056 Basel, Switzerland
    Prog Histochem Cytochem 39:131-82. 2004
    ..These studies indicate that the initial outgrowth of the Purkinje cell dendritic tree can occur in the absence of signals derived from afferent fibers, but is under control of PKC signaling...
  57. ncbi Toward an optimum system for intervertebral disc organ culture: TGF-beta 3 enhances nucleus pulposus and anulus fibrosus survival and function through modulation of TGF-beta-R expression and ERK signaling
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 31:884-90. 2006
    ..Rat lumbar discs comprising nucleus pulposus, annulus fibrosus, and cartilaginous endplates were cultured for 1 week in a specialized media containing either TGF-beta1 or TGF-beta3. Role of TGF-beta isoforms on cell function was evaluated...
  58. ncbi Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling
    J Ericson
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Cell 90:169-80. 1997
    ..2. Pax6 establishes distinct ventral progenitor cell populations and controls the identity of motor neurons and ventral interneurons, mediating graded Shh signaling in the ventral spinal cord and hindbrain...
  59. pmc Organotypic culture of physiologically functional adult mammalian retinas
    Amane Koizumi
    Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 2:e221. 2007
    ..Unfortunately, the tissue culture of the adult mammalian retina is difficult, mainly because of the high energy consumption of photoreceptors...
  60. ncbi Towards the development of a simplified long-term organ culture method for human scalp skin and its appendages under serum-free conditions
    Zhongfa Lu
    Department of Dermatology, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
    Exp Dermatol 16:37-44. 2007
    ..This pragmatic assay invites multiple uses, and may become a valuable tool for both skin and hair research...
  61. ncbi Neural crest induction in Xenopus: evidence for a two-signal model
    C LaBonne
    Division of Biology, Beckman Institute 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Development 125:2403-14. 1998
    ..Slug-expressing ectoderm will generate neural crest in the presence of Wnt or FGF-like signals, however, bypassing the need for BMP inhibition in this process. A two-step model for neural crest induction is proposed...
  62. doi High replication fitness and transmission efficiency of HIV-1 subtype C from India: Implications for subtype C predominance
    Milka A Rodriguez
    Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, 426 Parran Hall, GSPH, 130 DeSoto Street, Pittsburgh, PA 15261, USA
    Virology 385:416-24. 2009
    ..These data provide a model to explain the asymmetric distribution of subtype C over other subtypes in India...
  63. ncbi Microglia stimulate the invasiveness of glioma cells by increasing the activity of metalloprotease-2
    Darko S Markovic
    Cellular Neuroscience Group, Max Delbrück Center for Molecular Medicine MDC, Berlin, Germany
    J Neuropathol Exp Neurol 64:754-62. 2005
    ..Our data indicate that glioma cells stimulate microglial cells to increase breakdown of extracellular matrix and thereby promote tumor invasiveness...
  64. ncbi Essential role for Sonic hedgehog during hair follicle morphogenesis
    C Chiang
    Laboratory of Mammalian Genes and Development, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Dev Biol 205:1-9. 1999
    ..Our findings reveal an essential role for Shh during hair follicle morphogenesis, where it is required for normal advancement beyond the hair germ stage of development...
  65. ncbi A CaMKII-NeuroD signaling pathway specifies dendritic morphogenesis
    Brice Gaudilliere
    Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Neuron 41:229-41. 2004
    ..Our study also defines the CaMKII-NeuroD signaling pathway as a novel mechanism underlying activity-regulated dendritic growth that may play important roles in the developing and mature brain...
  66. ncbi Cell-intrinsic regulation of axonal morphogenesis by the Cdh1-APC target SnoN
    Judith Stegmuller
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Neuron 50:389-400. 2006
    ..These findings define Cdh1-APC and SnoN as components of a cell-intrinsic pathway that orchestrates axonal morphogenesis in a transcription-dependent manner in the mammalian brain...
  67. ncbi Inhibition of glycogen synthase kinase 3beta in sensory neurons in culture alters filopodia dynamics and microtubule distribution in growth cones
    Rebecca Owen
    The MRC Centre for Developmental Neurobiology, King s College London, Room 4 26B, New Hunts House, Guy s Campus, London SE1 1UL, UK
    Mol Cell Neurosci 23:626-37. 2003
    ..These results suggest that a major role of MAP1B in growing axons and growth cones is to regulate microtubule and actin filament stability. Furthermore, this function is regulated by phosphorylation of MAP1B by GSK-3beta...
  68. pmc Synaptic localization of a functional NADPH oxidase in the mouse hippocampus
    Maria V Tejada-Simon
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell Neurosci 29:97-106. 2005
    ..Taken together, our data suggest that NADPH oxidase is present in mouse hippocampus and might be the source of superoxide production required for LTP and memory function...
  69. ncbi The phenotype of limbal epithelial stem cells
    Edwin C Figueira
    Inflammatory Diseases Research Unit, University of New South Wales, Australia
    Invest Ophthalmol Vis Sci 48:144-56. 2007
    ..The purpose of this study was to identify phenotypic markers of human limbal stem cells in fetal and adult corneas...
  70. pmc Atoh1 directs the formation of sensory mosaics and induces cell proliferation in the postnatal mammalian cochlea in vivo
    Michael C Kelly
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Neurosci 32:6699-710. 2012
    ..Furthermore, Atoh1 also activates proliferation within the normally postmitotic cochlear epithelium. These results provide insight into the potential and limitations of Atoh1-mediated hair cell regeneration...
  71. pmc A quantitative spatiotemporal analysis of microglia morphology during ischemic stroke and reperfusion
    Helena W Morrison
    Georgia Health Sciences University, Augusta, GA 30912, USA
    J Neuroinflammation 10:4. 2013
    ..The purpose of this study was to quantitatively characterize the spatiotemporal pattern of microglia morphology during the evolution of cerebral injury after IS and reperfusion...
  72. ncbi miR-181a is an intrinsic modulator of T cell sensitivity and selection
    Qi jing Li
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 129:147-61. 2007
    ..Importantly, higher miR-181a expression correlates with greater T cell sensitivity in immature T cells, suggesting that miR-181a acts as an intrinsic antigen sensitivity "rheostat" during T cell development...
  73. doi CD11c-expressing cells reside in the juxtavascular parenchyma and extend processes into the glia limitans of the mouse nervous system
    Carolin Prodinger
    Institute of Clinical Neuroanatomy, Johann Wolfgang Goethe University, 60590 Frankfurt Main, Germany
    Acta Neuropathol 121:445-58. 2011
    ..Dysbalance in their recruitment/development may be an initial step in the pathogenesis of chronic (autoimmune) neuroinflammatory diseases such as MS...
  74. ncbi Enhanced transcription of contractile 5-hydroxytryptamine 2A receptors via extracellular signal-regulated kinase 1/2 after organ culture of rat mesenteric artery
    Yong xiao Cao
    Division of Experimental Vascular Research, Department of Medicine, Lund University, Sweden
    Basic Clin Pharmacol Toxicol 96:282-8. 2005
    ..These data reveal a role of ERK1/2 in up-regulation of 5-HT2A receptors and suggest a possibility to inhibit the enhanced responses to 5-HT by inhibition of the ERK1/2 pathway...
  75. pmc Distinct cis-regulatory elements from the Dlx1/Dlx2 locus mark different progenitor cell populations in the ganglionic eminences and different subtypes of adult cortical interneurons
    Noel Ghanem
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
    J Neurosci 27:5012-22. 2007
    ..These data suggest that interneuron subtypes use distinct combinations of Dlx1/Dlx2 enhancers from the time they are specified through adulthood...
  76. doi Thyrotropin releasing hormone (TRH): a new player in human hair-growth control
    Erzsébet Gáspár
    Department of Dermatology, University of Lubeck, Ratzeburger Allee 160 D 23538 Lübeck, Germany
    FASEB J 24:393-403. 2010
    ..Human HFs provide an excellent discovery tool for identifying and dissecting nonclassical functions of TRH and TRH-mediated signaling in situ, which emerge as novel players in human epithelial biology...
  77. pmc The contribution of inositol 1,4,5-trisphosphate and ryanodine receptors to agonist-induced Ca(2+) signaling of airway smooth muscle cells
    Yan Bai
    Dept of Physiology, Univ of Massachusetts Medical School, Worcester, 01655, USA
    Am J Physiol Lung Cell Mol Physiol 297:L347-61. 2009
  78. ncbi Onset of diabetes in Zucker diabetic fatty (ZDF) rats leads to improved recovery of function after ischemia in the isolated perfused heart
    Peipei Wang
    Division of Cardiovascular Disease, Department of Medicine, The Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294 0005, USA
    Am J Physiol Endocrinol Metab 286:E725-36. 2004
    ..These data demonstrate that, although the development of type 2 diabetes leads to progressive contractile and metabolic abnormalities during normoxia and LFI, it was not associated with increased susceptibility to ischemic injury...
  79. pmc An in vitro model of adult mammalian nerve repair
    Alka Vyas
    Department of Orthopaedic Surgery, Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    Exp Neurol 223:112-8. 2010
    ..The flexibility and control offered by this technique should thus make it a useful tool for the study of regeneration biology...
  80. doi Thyrotropin-releasing hormone and oestrogen differentially regulate prolactin and prolactin receptor expression in female human skin and hair follicles in vitro
    E A Langan
    Dermatological Sciences, University of Manchester, Manchester Academic Health Science Centre, Manchester M6 8HD, UK
    Br J Dermatol 162:1127-31. 2010
    ..However, how the expression of PRL and PRL receptor (PRLR) is regulated in human skin is unknown...
  81. pmc Dynamic compression effects on intervertebral disc mechanics and biology
    Casey L Korecki
    School of Engineering, College of Engineering and Mathematical Sciences, University of Vermont, Burlington, VT, USA
    Spine (Phila Pa 1976) 33:1403-9. 2008
    ..A bovine intervertebral disc organ culture model was used to study the effect of dynamic compression magnitude on mechanical behavior and measurement of biosynthesis rate, cell viability, and mRNA expression...
  82. doi Human placenta expresses and secretes NKG2D ligands via exosomes that down-modulate the cognate receptor expression: evidence for immunosuppressive function
    Malin Hedlund
    Department of Clinical Immunology, Umea University, Sweden
    J Immunol 183:340-51. 2009
    ..These findings highlight a role for NKG2D ligand-bearing placental exosomes in the fetal immune escape and support the view of placenta as a unique immunosuppressive organ...
  83. ncbi NMDA receptor-dependent high-frequency network oscillations (100-300 Hz) in rat hippocampal slices
    Costas Papatheodoropoulos
    University of Patras, Department of Physiology, Medical School, 26504 Rio, Patras, Greece
    Neurosci Lett 414:197-202. 2007
    ..These data indicate that synaptic transmission provided by solely NMDARs can sustain the generation of high-frequency network oscillations, which display distinct characteristics in CA3 and CA1 subregions...
  84. pmc Vascular endothelial growth factor-A is a survival factor for retinal neurons and a critical neuroprotectant during the adaptive response to ischemic injury
    Kazuaki Nishijima
    R and D Consultants, Barnet, Herts, UK
    Am J Pathol 171:53-67. 2007
    ..These findings have implications for both neural pathologies and ocular vascular diseases, such as diabetic retinopathy and age-related macular degeneration...
  85. ncbi Mechanism of guanosine-induced neuroprotection in rat hippocampal slices submitted to oxygen-glucose deprivation
    Scheyla P B Oleskovicz
    Departamento de Bioquimica, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Trindade, 88040 900 Florianopolis, SC, Brazil
    Neurochem Int 52:411-8. 2008
    ..Therefore, this study shows guanosine is acting via K(+) channels activation, depending on extracellular Ca(2+) levels and via modulation of the PKA, PKC, MEK and/or PI-3 K pathways...
  86. pmc Maintaining network activity in submerged hippocampal slices: importance of oxygen supply
    Norbert Hajos
    Department of Cellular and Network Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony u 43, 1083 Budapest, Hungary
    Eur J Neurosci 29:319-27. 2009
    ..These improvements to the recording conditions enable detailed studies of neurons under more realistic conditions of network activity, which are essential for a better understanding of neuronal network operation...
  87. ncbi Hair cell regeneration in sensory epithelia from the inner ear of a urodele amphibian
    Ruth R Taylor
    UCL Centre for Auditory Research, University College London, London WC1X 8EE, United Kingdom
    J Comp Neurol 484:105-20. 2005
    ..A similar mechanism has been proposed for the hair cell recovery phenomenon observed in the vestibular organs of mammals...
  88. pmc Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum
    Takaki Miyata
    Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan
    Neural Dev 5:23. 2010
    ..Furthermore, it is not known what nascent Purkinje cells look like in vivo. When and how Purkinje cells start axonogenesis must also be elucidated...
  89. doi GSK3β negatively regulates oligodendrocyte differentiation and myelination in vivo
    Kasum Azim
    Institute of Biology and Biomedical Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, United Kingdom
    Glia 59:540-53. 2011
    ..This study identifies GSK3β as a profound negative regulator of OL differentiation that contributes to inefficient regeneration of OLs and myelin repair in demyelination...
  90. pmc A guide to Ussing chamber studies of mouse intestine
    Lane L Clarke
    Univ of Missouri, 324 D Dalton Cardiovascular Research Ctr, 134 Research Park Dr, Columbia, MO 65211 3300, USA
    Am J Physiol Gastrointest Liver Physiol 296:G1151-66. 2009
    ..The purpose of the review is to provide a practical guide for investigators who are new to the Ussing chamber method...
  91. ncbi Planar and vertical signals control cellular differentiation and patterning in the mammalian cochlea
    Mireille Montcouquiol
    Section on Developmental Neuroscience, National Institute on Deafness and Other Communication Disorders National Institutes of Health, Rockville, Maryland 20850, USA
    J Neurosci 23:9469-78. 2003
    ..Finally, we demonstrate that the temporal gradient of cellular differentiation is not dependent on planar signals within the developing sensory epithelium...
  92. ncbi Death is the major fate of medial edge epithelial cells and the cause of basal lamina degradation during palatogenesis
    Rodrigo Cuervo
    Departament of Developmental Genetics and Molecular Physiology, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos 62210, Mexico
    Development 131:15-24. 2004
    ..We conclude that MES degeneration occurs almost uniquely by cell death, and for the first time we show that this process can activate basal lamina degradation during a developmental process...
  93. doi Prospective clinical evaluation of McCarey-Kaufman and organ culture cornea preservation media: 14-year follow-up
    Wilhelmina J Rijneveld
    Cornea Bank Amsterdam, Neuro Institute for Neuroscience, Amsterdam, The Netherlands
    Cornea 27:996-1000. 2008
    ..To compare the outcome of corneal grafts preserved in McCarey-Kaufman (MK) medium versus organ culture after penetrating keratoplasties...
  94. pmc Ephrin-B2 elicits differential growth cone collapse and axon retraction in retinal ganglion cells from distinct retinal regions
    Timothy J Petros
    Department of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA
    Dev Neurobiol 70:781-94. 2010
    ..The detailed characterization of growth cone behavior in this study comprises an assay for the study of Eph signaling in RGCs, and provides insight into the phenomena of growth cone collapse and axon retraction in general...
  95. doi Evaluation of cell damage in organotypic hippocampal slice culture from adult mouse: a potential model system to study neuroprotection
    Tao Su
    Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and Ministry of Education of China, Institute of Neuroscience and The Second Affiliated Hospital of GuangZhou Medical University, Guangzhou, China
    Brain Res 1385:68-76. 2011
    ..It is suggested that OHSCs from adult mice might provide an alternative model system for neuronal degeneration, suitable for studying physiological factors and pharmacological compounds contributing to neuronal survival...
  96. doi Optimizing the deswelling process of organ-cultured corneas
    Armin H Wolf
    Department of Ophthalmology, Ludwig Maximilians Universitat, Muenchen, Germany
    Cornea 28:524-9. 2009
    ..We investigated the minimal deswelling time necessary and analyzed endothelial cell loss...
  97. ncbi Interleukin-6 promotes sprouting and functional recovery in lesioned organotypic hippocampal slice cultures
    David Hakkoum
    Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland
    J Neurochem 100:747-57. 2007
    ..Taken together these data indicate that IL-6 plays an important role in CNS repair mechanisms by promoting regrowth and axon regeneration...
  98. pmc Neuroprotective properties of levosimendan in an in vitro model of traumatic brain injury
    Anna B Roehl
    Department of Anaesthesiology, RWTH Aachen University Hospital, Pauwelsstrase 30, 52074 Aachen, Germany
    BMC Neurol 10:97. 2010
    ..We investigated the neuroprotective properties of levosimendan, a novel inodilator, in an in vitro model of traumatic brain injury...
  99. doi Mechanisms supporting transfer of inhibitory signals into the spike output of spontaneously firing cerebellar nuclear neurons in vitro
    Christine M Pedroarena
    Department of Cognitive Neurology, Hertie Institute for Clinical Brain Research, University of Tubingen, Otfried Müller Str 27, Tubingen, Germany
    Cerebellum 9:67-76. 2010
  100. doi Immunotoxic depletion of microglia in mouse hippocampal slice cultures enhances ischemia-like neurodegeneration
    Maria Montero
    Anatomy and Neurobiology, University of Southern Denmark, Denmark
    Brain Res 1291:140-52. 2009
  101. doi Spontaneous neuronal burst discharges as dependent and independent variables in the maturation of cerebral cortex tissue cultured in vitro: a review of activity-dependent studies in live 'model' systems for the development of intrinsically generated bioel
    Michael A Corner
    Netherlands Institute for Brain Research, Amsterdam, The Netherlands
    Brain Res Rev 59:221-44. 2008

Research Grants73

  1. Rac GTPase signaling during embryonic salivary gland branching morphogenesis
    Sharon Sequeira; Fiscal Year: 2012
  2. Bioassay for Breast Cancer Prevention
    JoEllen Welsh; Fiscal Year: 2010
  3. Epigenetic effects of developmental endocrine disruptor exposure in the ovary
    Mehmet Uzumcu; Fiscal Year: 2010
    ..A mechanistic understanding of the epigenetic effects of EDC exposure can lead to better prevention, diagnosis, and therapies, thereby improving public health and reducing health care costs. ..
  4. The Mechanism of Cryptorchidism in LH Receptor Knockout Animals
    Zhenmin Lei; Fiscal Year: 2012
    ..The current proposal is focusing on determining androgen downstream target genes and subsequent signaling network during the process of inguinoscrotal testis descent, which may provide new insight into the causes of testis maldescent. ..
  5. Novel Drug VS-105 for Treatment of Osteoporosis
    Jinshyun Ruth Wu-Wong; Fiscal Year: 2013
    ..Assuming Vidasym's VS-105 has a modest 5% penetration into the osteoporosis market, the estimated annual sales will be US$0.6+ billion. ..
  6. Functional characterization of the Bronx waltzer deafness gene
    Botond Banfi; Fiscal Year: 2013
  7. Effect of Maspin on Corneal Heme-and lymph- angiogenesis
    Sally S Twining; Fiscal Year: 2013
    ..The results will guide the generation and initial testing of maspin mutants and peptides as potential modalities for the treatment of neovascular diseases. ..
  8. Role of Biophysical Cues in the Pathogenesis of Glaucoma
    Paul Russell; Fiscal Year: 2013
    ..We believe this will allow researchers to improve the relevancy of their results with cultured cells;and additionally, we believe understanding of these cues will lead to novel therapeutic targets for glaucoma treatment. ..
    Fu Shin X Yu; Fiscal Year: 2013
    Michael S Wolin; Fiscal Year: 2010
    Ted S Acott; Fiscal Year: 2012
    ..These studies are focused at understanding the molecular nature of the aqueous humor outflow pathway, which is normally responsible for avoiding glaucoma and which becomes obstructed to produce glaucoma. ..
    Haydee E P Bazan; Fiscal Year: 2010
    ..If our hypothesis and its corollaries are correct, then PAF antagonists can become effective therapeutic tools for maintaining the transparency and integrity of the cornea. ..
    MARC RANDALL HAMMERMAN; Fiscal Year: 2013
    ..The WU Renal Division has a longstanding commitment to train individuals from minority groups and several are longstanding members of its faculty. ..
    Samir S El-Dahr; Fiscal Year: 2012
  15. Mechanism of Gene Repression in the Kidney
    Sanjeevkumar R Patel; Fiscal Year: 2013
    ..3. Identify candidate Pax2 dependent genes that are modulated by Grg4. 4. Examine the phenotype of Grg4 and associated proteins over-expression/knockdown in organ culture. ..
  16. CFTR inhibitors for therapy of polycystic kidney disease
    Alan S Verkman; Fiscal Year: 2010
    ..This new form of therapy might ultimately prolong kidney function in polycystic kidney disease. ..
  17. Embryonic Development of the Mammalian Epididymis
    Barry T Hinton; Fiscal Year: 2013
    ..A combination of genetically modified mice, modern imaging techniques, in vitro organ culture techniques, and gene modifying approaches will be used to test the hypotheses outlined in the following three ..
  18. Transcriptional Regulation of Indian Hedgehog Expression in Chondrocytes
    Xiangli Yang; Fiscal Year: 2012
    ..In this proposal, we intend to investigate this important biological question through systematic molecular and genetic studies. ..
  19. Cutaneous wound remodeling by re-differentiating keratinocytes expressing CXCR3 l
    ARTHUR HUEN; Fiscal Year: 2013
    ..Understanding the control of these signals may lead to better ways to treat chronic wounds and to more rapidly restore skin function and appearance after trauma. ..
    Paul Kaufman; Fiscal Year: 2009
    ..Such knowledge will contribute to the development of new antiglaucoma drugs and perhaps to understanding the underlying biochemical, physiological, and morphological basis of the human open angle glaucomas. ..
    Peter Igarashi; Fiscal Year: 2013
  22. Role of Inhibitory SMADs in Calcific Aortic Valve Disease
    Yin Tintut; Fiscal Year: 2013
    ..Results of the proposed work will harness the regulatory power of I-SMADs for development of preventive measures and medical treatments for CAVD. (End Abstract) ..
  23. BMP Regulation of TGF-b2 in the Trabecular Meshwork
    Robert J Wordinger; Fiscal Year: 2012
    ..This study is relevant because it will examine the role of local growth factors in the control of eye pressure and may lead to new treatments for glaucoma. ..
  24. Deguelin in therapy of triple negative breast cancer
    Rajendra G Mehta; Fiscal Year: 2013
  25. Exercise, Diabetes, &Coronary Smooth Muscle Ca2+
    Michael Sturek; Fiscal Year: 2013
    ..Drugs currently approved for humans are very likely to find new uses in treatment of coronary disease. We are uniquely positioned to benefit the prevention and regression of coronary disease, which will improve health in the U.S. ..
  26. Short-Chain Dehydrogenases in Retinol/Sterol Metabolism
    Natalia Y Kedishvili; Fiscal Year: 2013
  27. Novel role of AQP2 in cell migration and kidney tubular injury and repair
    HUA A LU; Fiscal Year: 2013
  28. Growth Factor Signaling in Mouse Palatogensis
    YiPing Chen; Fiscal Year: 2013
    ..The results obtained from this study will provide fundamental information for our understanding of cleft palate formation in humans, and provide an insight for the genetic prevention and therapy of the human cleft palate. ..
  29. Gdnf and endothelin-3 regulate colorectal enteric nervous system development
    Allan M Goldstein; Fiscal Year: 2013
    ..abstract_text> ..
  30. The Role and Mechanisms of microRNAs in Diabetic Cornea
    Mehrnoosh Saghizadeh; Fiscal Year: 2013
    ..Normal and diabetic human autopsy eyes will be purchased from the National Disease Research Interchange (NDRI), a national supplier of donor human research tissue. All tissues are obtained in our lab without donor identity. ..
  31. Mechanisms of FGFR2 Signaling in Salivary Gland Branching Morphogenesis
    Veraragavan P Eswarakumar; Fiscal Year: 2013
    ..The results of these studies may enable the design of novel methods for salivary gland regeneration using FGFR2b mediated pathways to regulate progenitor cell differentiation and morphogenesis. ..
  32. Molecular Regulation of Cancer Progression
    Alan Wells; Fiscal Year: 2013
    ..Lastly, in the longer term, select molecular fingerprints or signaling cascades could be developed as targets for future agents. ..
  33. Development Estrogenization of the Rat Prostate Gland
    Gail S Prins; Fiscal Year: 2010
    ..Results will further define the mechanisms of estrogen's actions on the prostate gland during early development and lead to a better understanding of the hormonal and developmental basis for abnormal prostate growth with aging...
    PETER DANA YURCHENCO; Fiscal Year: 2013
    ..Lm[unreadable]LN also adheres to cell surfaces. We will explore interacting sequences in LN and LE domains, dissect to role of cell adhesion, and evaluate Pierson syndrome laminin [unreadable]2LN domain mutations. ..
    Liza A Pon; Fiscal Year: 2010
    ..As a compo- nent of our Confocal and Specialized Microscopy Facility, the instrument will be accessible to Major Users and others throughout the University, promoting collaboration and exchange of ideas. ..
  36. Developmental Regulation of Collective Cell Migration
    Denise J Montell; Fiscal Year: 2013
  37. CMV-Induced Embryonic Cochlear Pathogenesis
    TINA contact JASKOLL; Fiscal Year: 2011
  38. Molecular Patterning of Mammalian Dentition
    Rulang Jiang; Fiscal Year: 2013
    ..Data from this project will significantly improve the current understanding of the molecular mechanisms of tooth development and multiple human birth defects and will facilitate development of new therapeutic strategies. ..
    James B Kaper; Fiscal Year: 2013
    ..abstract_text> ..
  40. High throughput screening to find inhibitors of pathogenic pemphigus antibodies
    John R Stanley; Fiscal Year: 2012
    ..Current therapy is to suppress the immune system, which results in many potential adverse effects. This proposal seeks therapy directly targeted only to the autoantibodies that actually cause the blisters in this disease. ..
  41. Cadherin-11 Regulation of Fibroblast-like Synoviocytes in Synovial Inflammation
    Sandeep K Agarwal; Fiscal Year: 2012
  42. Molecular mechanisms in steroid-induced glaucoma.
    John Danias; Fiscal Year: 2012
    ..abstract_text> ..
  43. Organ engineering based on perfusion decellularized matrix
    Harald C Ott; Fiscal Year: 2011
    ..Organ engineering based on patient derived cells could offer an alternative to transplantation, potentially alleviating donor organ shortage, and the need for immunosuppression. ..
  44. Mediators of Fibrosis in Scleroderma Skin and Lung
    Carol A Feghali-Bostwick; Fiscal Year: 2012
    ..Our studies will also generate new targets for the development of future therapies for the treatment of systemic sclerosis and other fibrotic diseases. ..
  45. Receptor Tyrosine Kinases in Mechanotransduction
    Zheng Gen Jin; Fiscal Year: 2009
    ..In this pathway, VEGFR2 may emerge as a central mediator for laminar flow atheroprotective effects, which will provide new insight into the prevention of atherosclerotic vascular disease. ..
  46. The Mechanical Basis of Primary Open Angle Glaucoma
    Mark Johnson; Fiscal Year: 2013
    ..If we can determine the cause of the elevated pressure, then we may be able to develop a more effective pressure-lowering treatment or cure for this debilitating disease. ..
  47. Plasmacytoid Dendritic Cells in HIV Pathogenesis
    Patricia Fitzgerald-Bocarsly; Fiscal Year: 2013
    ..Together, the studies proposed in this application are anticipated to provide valuable new information on the biology of human pDC and the mechanisms underlying their dysfunction in HIV-1 infection. ..
  48. Determining the Effect of Altered Pulse Pressure on Artery Structure and Function
    DANIKA HAYMAN; Fiscal Year: 2010
    ..Ultimately this knowledge will improve vascular disease treatment. ..
  49. Retrocyclins: Circular Defensins Active Against HIV-1
    Alexander M Cole; Fiscal Year: 2010
    ..Availability of such agents, in the form of a cream or gel, would empower vulnerable sexual partners by providing them with an invisible, effective means of protection. ..
  50. Reproductive Hormones and their impact on HIV-1 acquisition
    Bharat Ramratnam; Fiscal Year: 2013
  51. Biomechanical Mechanisms of Artery Tortuosity
    Hai Chao Han; Fiscal Year: 2013
    ..Understanding the biomechanical mechanisms how tortuosity initiates and develop will reveal its role in vascular disease and lead to new understanding of vascular disease and set a basis for development of new treatment. ..
  52. Mechanisms of Endothelial Cell Hyperpolarization
    Sean P Marrelli; Fiscal Year: 2012
    ..These studies will define this mechanism of blood flow control and thus provide novel therapeutic strategies for regulating cerebral blood flow in health and disease states. ..
  53. Novel Biomaterials as Sealants for Applications in Annulus Fibrosus Repair
    Clare Canal Guterl; Fiscal Year: 2012
    ..segment following repair, and to assess the sealant-tissue interface for cell growth and transport using organ culture techniques. The overall hypothesis is that an AF adhesive sealant can be optimized to be cytocompatible, of ..
  54. The Role of SPARC in Procollagen Processing in the Periodontal Ligament
    ..The training plan proposed here will test the above hypothesis and prepare me for a career as an academic scientist. ..
  55. The Role of GSK-3beta in Palate Development and Fusion
    Michael Longaker; Fiscal Year: 2009
    ..These facts are significant and while certain genes and environmental factors have been associated with the development of cleft palate, the cause in the majority of cases remains unknown and further study is extremely important. ..
    Ted S Acott; Fiscal Year: 2012
    ..These studies are focused at understanding what causes glaucoma and why many people do not develop this disease, even at advanced ages. ..
  57. Developmental patterning of the cochlea
    Takahiro Ohyama; Fiscal Year: 2013
    ..Our proposed research will significantly contribute to the understanding of the key mechanisms that control the cell fate decision of all sensory and non-sensory cell types in the mammalian cochlea. ..
    Karen M Lyons; Fiscal Year: 2013
  59. GDNF/Ret Regulation of Ureteric Bud Morphogenesis
    ADAM I PACKARD; Fiscal Year: 2012
  60. Spatiotemporal Modulation of PIP[3]Signaling in Prostatic Tubulogenesis
    Tamara L Lotan; Fiscal Year: 2013
    ..This pathway is of particular interest because there are numerous drugs available for modulating its activity that may be useful for the treatment of human prostate diseases once we fully understand how the pathway works. ..
    Curtis R Brandt; Fiscal Year: 2013
    ..During the previous funding period a total of 28 qualifying NEI grants were assisted. As of the submission date, 188 peer reviewed papers have involved use of the core. ..
  62. Computer-aided Development of a Liver Organ Culture System
    Korkut Uygun; Fiscal Year: 2009
    ..A major reason of liver related deaths is the limited donor pool. This project aims to improve public health by utilizing currently discarded donor livers by reconditioning them to be transplantable with success. x ..
  63. Constrictor / Mitogen Induced Remodeling of Cerebral Arterioles
    JAY HUMPHREY; Fiscal Year: 2009
    ..This study will be the first to propose and test this new hypothesis as well as a potential means to block this remodeling. ..
    Rajesh Agarwal; Fiscal Year: 1999
    ..The results of the proposed studies will identify a new agent with significant anti-tumor promoting effect, and will define in particular the Ha-ras mediated mechanism of such protective effect. ..
    Joseph Majzoub; Fiscal Year: 1993
    ..Transgenic Brattleboro rats bearing mutant rat VP genes will also be prepared to evaluate the impact of dysfunctional VP genes on the mRNA tail and secretory rhythms...
    Abraham Spector; Fiscal Year: 2001
    ..This is the first finding of tyrosine phosphorylation of the crystallins. We intend to further confirm this observation and attempt to determine the effect of such phosphorylation. ..
    SUSAN FRIED; Fiscal Year: 1992
    ..These studies will allow comparison of in vivo and in vitro LPL regulation in humans and will provide insight into the molecular mechanisms controlling site-specific fat accumulation...
    Russell Hall; Fiscal Year: 2005
    ..These studies will provide insights into the pathogenesis of DH and isolated GSE, the relationship between the MIR and the skin and factors important in controlling the mucosal immune response in man. ..
    GARY GORBY; Fiscal Year: 1999
    ..Morphometric comparisons of these mutants to E. coli expressing wild-type PIA in the FTOC model will confirm the pathogenic role of these regions. ..
  70. Vascular Tissue Invasion by Porphyromonas gingivalis
    Ling Li; Fiscal Year: 2005
    ..The completion of this study will contribute to the long-term goal of understanding this bacterially induced vascular inflammation and identification of new therapeutic targets. ..
    John Samples; Fiscal Year: 1991
    ..To date, little work has been done on the regulation of trabecular ECM. Using growth factors with a range of methods will add valuable insights into the behavior of the cells of the trabecular meshwork...
    Amr Moursi; Fiscal Year: 2001
    ..Together, these studies will provide important data which could be used to develop new therapeutic adjuncts in the treatment of craniosynostosis. ..
  73. HGF Induces Robust Human Thymopoiesis in Mice
    Jan Nolta; Fiscal Year: 2004