cell culture techniques

Summary

Summary: A technique for maintaining or growing CELLS in vitro. Cultures of dispersed cells derived directly from fresh TISSUES are called primary cell cultures. Cultures may also derive from established CELL LINE usually stored frozen.

Top Publications

  1. ncbi Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
    M Dominici
    Laboratory of Cell Biology and Advanced Cancer Therapy, Oncology Hematology Department, University of Modena and Reggio Emilia, Modena, Italy
    Cytotherapy 8:315-7. 2006
  2. ncbi Embryonic stem cell lines derived from human blastocysts
    J A Thomson
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
    Science 282:1145-7. 1998
  3. pmc Human adipose tissue is a source of multipotent stem cells
    Patricia A Zuk
    Department of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Biol Cell 13:4279-95. 2002
  4. ncbi Derivation of pluripotent epiblast stem cells from mammalian embryos
    I Gabrielle M Brons
    Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 0XY, UK
    Nature 448:191-5. 2007
  5. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
  6. ncbi New cell lines from mouse epiblast share defining features with human embryonic stem cells
    Paul J Tesar
    Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 448:196-9. 2007
  7. ncbi Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
    Masato Nakagawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nat Biotechnol 26:101-6. 2008
  8. pmc Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
    Stuart M Chambers
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10065, USA
    Nat Biotechnol 27:275-80. 2009
  9. pmc Three-dimensional culture models of normal and malignant breast epithelial cells
    Genee Y Lee
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nat Methods 4:359-65. 2007
  10. ncbi Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood
    David A Ingram
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202, USA
    Blood 104:2752-60. 2004

Detail Information

Publications261 found, 100 shown here

  1. ncbi Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
    M Dominici
    Laboratory of Cell Biology and Advanced Cancer Therapy, Oncology Hematology Department, University of Modena and Reggio Emilia, Modena, Italy
    Cytotherapy 8:315-7. 2006
    ....
  2. ncbi Embryonic stem cell lines derived from human blastocysts
    J A Thomson
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
    Science 282:1145-7. 1998
    ..These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine...
  3. pmc Human adipose tissue is a source of multipotent stem cells
    Patricia A Zuk
    Department of Surgery and Orthopedics, Regenerative Bioengineering and Repair Laboratory, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Biol Cell 13:4279-95. 2002
    ..Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression...
  4. ncbi Derivation of pluripotent epiblast stem cells from mammalian embryos
    I Gabrielle M Brons
    Department of Surgery and Cambridge Institute for Medical Research, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 0XY, UK
    Nature 448:191-5. 2007
    ..Epiblast stem cells provide a valuable experimental system for determining whether distinctions between mouse and human embryonic stem cells reflect species differences or diverse temporal origins...
  5. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
    ....
  6. ncbi New cell lines from mouse epiblast share defining features with human embryonic stem cells
    Paul J Tesar
    Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 448:196-9. 2007
    ..These results show that epiblast cells can be maintained as stable cell lines and interrogated to understand how pluripotent cells generate distinct fates during early development...
  7. ncbi Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
    Masato Nakagawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nat Biotechnol 26:101-6. 2008
    ..The protocol also enabled efficient isolation of iPS cells without drug selection. Furthermore, we generated human iPS cells from adult dermal fibroblasts without MYC...
  8. pmc Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling
    Stuart M Chambers
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10065, USA
    Nat Biotechnol 27:275-80. 2009
    ..Noggin/SB431542-based neural induction should facilitate the use of hES and hiPS cells in regenerative medicine and disease modeling and obviate the need for protocols based on stromal feeders or embryoid bodies...
  9. pmc Three-dimensional culture models of normal and malignant breast epithelial cells
    Genee Y Lee
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nat Methods 4:359-65. 2007
    ..Alternatively, the 3D 'on-top' assay, in which cells are cultured on top of a thin lrECM gel overlaid with a dilute solution of lrECM, may be used as described in Box 2 (Fig. 1 and Fig. 2)...
  10. ncbi Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood
    David A Ingram
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202, USA
    Blood 104:2752-60. 2004
    ....
  11. ncbi Taking cell-matrix adhesions to the third dimension
    E Cukierman
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Science 294:1708-12. 2001
    ..These distinctive in vivo 3D-matrix adhesions differ in structure, localization, and function from classically described in vitro adhesions, and as such they may be more biologically relevant to living organisms...
  12. ncbi Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
    Toshiro Sato
    Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
    Nature 459:262-5. 2009
    ..We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche...
  13. pmc Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture
    P M Gilbert
    Baxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 329:1078-81. 2010
    ..Our studies provide novel evidence that by recapitulating physiological tissue rigidity, propagation of adult muscle stem cells is possible, enabling future cell-based therapies for muscle-wasting diseases...
  14. ncbi Isolation of multipotent mesenchymal stem cells from umbilical cord blood
    Oscar K Lee
    Department of Orthopaedics and Traumatology, Veterans General Hospital Taipei, Taipei 11221, Taiwan
    Blood 103:1669-75. 2004
    ..In conclusion, umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical waste. It can serve as an alternative source of mesenchymal stem cells to bone marrow...
  15. ncbi Induction of pluripotent stem cells from fibroblast cultures
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara machi, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Nat Protoc 2:3081-9. 2007
    ..The cells, named induced pluripotent stem (iPS) cells, can be differentiated into three germ layers and committed to chimeric mice. Here we describe detailed methods and tips for the generation of iPS cells...
  16. pmc The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression
    Paraic A Kenny
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    Mol Oncol 1:84-96. 2007
    ..We further demonstrate that consistent differences in genes encoding signal transduction proteins emerge when even tumor cells are cultured in 3D microenvironments...
  17. ncbi The third dimension bridges the gap between cell culture and live tissue
    Francesco Pampaloni
    Francesco Pampaloni, Emmanuel G Reynaud and Ernst H K Stelzer are at the Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Rev Mol Cell Biol 8:839-45. 2007
    ..We believe that 3D cultures will have a strong impact on drug screening and will also decrease the use of laboratory animals, for example, in the context of toxicity assays...
  18. ncbi Modeling tissue morphogenesis and cancer in 3D
    Kenneth M Yamada
    Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 130:601-10. 2007
    ..These models may help to accelerate translational research in cancer biology and tissue engineering...
  19. ncbi Mesenchymal progenitor cells in human umbilical cord blood
    A Erices
    Unidad de Biologia Celular, INTA, Universidad de Chile, Santiago, Chile
    Br J Haematol 109:235-42. 2000
    ..Our results suggest that preterm, as compared with term, cord blood is richer in mesenchymal progenitors, similar to haematopoietic progenitors...
  20. ncbi Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood
    Wolfgang Wagner
    Department of Medicine V, University of Heidelberg, Heidelberg, Germany
    Exp Hematol 33:1402-16. 2005
    ..Whereas surface antigen markers have failed to precisely define this population, microarray analysis might provide a better tool for characterization of MSC...
  21. ncbi Direct conversion of human fibroblasts to multilineage blood progenitors
    Eva Szabo
    Stem Cell and Cancer Research Institute, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
    Nature 468:521-6. 2010
    ....
  22. ncbi Isolation of amniotic stem cell lines with potential for therapy
    Paolo De Coppi
    Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157 1094, USA
    Nat Biotechnol 25:100-6. 2007
    ....
  23. ncbi Cells on chips
    Jamil El-Ali
    Department of Chemical Engineering, Center for Cell Decision Processes, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 442:403-11. 2006
    ..Highly integrated microdevices show great promise for basic biomedical and pharmaceutical research, and robust and portable point-of-care devices could be used in clinical settings, in both the developed and the developing world...
  24. pmc Robust hepatitis C virus infection in vitro
    Jin Zhong
    Departments of Molecular and Experimental Medicine and Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 102:9294-9. 2005
    ..This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen...
  25. ncbi Telocytes in human term placenta: morphology and phenotype
    Laura Suciu
    Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
    Cells Tissues Organs 192:325-39. 2010
    ..In conclusion, this study confirms the presence of TC in human term placenta and provides their ultrastructural and immunophenotypic characterization...
  26. ncbi Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511
    Sergey Rodin
    Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
    Nat Biotechnol 28:611-5. 2010
    ..This xeno-free and feeder-free system may be useful for the development of cell lineages for therapeutic purposes...
  27. ncbi Development of definitive endoderm from embryonic stem cells in culture
    Atsushi Kubo
    The Carl C Icahn Center for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Development 131:1651-62. 2004
    ..These findings demonstrate that ES cells can generate endoderm in culture and, as such, establish this differentiation system as a unique murine model for studying the development and specification of this germ layer...
  28. ncbi In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability
    Aboulghassem Shahdadfar
    Institute of Immunology, Rikshospitalet University Hospital, 0027 Oslo, Norway
    Stem Cells 23:1357-66. 2005
    ..Thus, hMSCs may be expanded rapidly and with stable gene expression in AS in the absence of growth factors, whereas FBS induces a more differentiated and less stable transcriptional profile...
  29. ncbi Feeder-independent culture of human embryonic stem cells
    Tenneille E Ludwig
    Genome Center of Wisconsin, University of Wisconsin Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA
    Nat Methods 3:637-46. 2006
  30. ncbi Generation of pluripotent stem cells from adult human testis
    Sabine Conrad
    Institute of Anatomy, Department of Experimental Embryology, Tubingen, Germany
    Nature 456:344-9. 2008
    ....
  31. pmc Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge
    Maria Bokhari
    School of Biological and Biomedical Science, Durham University, South Road, Durham DH1 3LE, UK
    J Anat 211:567-76. 2007
    ..This technology has potential applications for toxicity testing and drug screening...
  32. ncbi Efficient differentiation of human embryonic stem cells to definitive endoderm
    Kevin A D'Amour
    CyThera Inc, 3550 General Atomics Ct, San Diego, California 92121, USA
    Nat Biotechnol 23:1534-41. 2005
    ..These findings may facilitate the use of hES cells for therapeutic purposes and as in vitro models of development...
  33. pmc Germline competent embryonic stem cells derived from rat blastocysts
    Ping Li
    Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Cell 135:1299-310. 2008
    ..Establishment of authentic rat ES cells will make possible sophisticated genetic manipulation to create models for the study of human diseases...
  34. ncbi Isolation of multipotent adult stem cells from the dermis of mammalian skin
    J G Toma
    Center for Neuronal Survival, Montreal Neurological Institute, McGill University, 3801 rue University, Montreal, Quebec, Canada H3A 2B4
    Nat Cell Biol 3:778-84. 2001
    ....
  35. ncbi Matrigel: basement membrane matrix with biological activity
    Hynda K Kleinman
    NIH, NIDCR, 30 433, 30 Convent Dr MSC 4370, Bethesda, MD 20892 4370, USA
    Semin Cancer Biol 15:378-86. 2005
    ..In vivo, it is used for measuring angiogenic inhibitors and stimulators, to improve graft survival, repair damaged tissues, and increase tumor growth...
  36. ncbi A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells
    Outi Hovatta
    Department of Clinical Science, Karolinska Institutet, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    Hum Reprod 18:1404-9. 2003
    ....
  37. pmc Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate
    Nathaniel Huebsch
    School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, USA
    Nat Mater 9:518-26. 2010
    ....
  38. ncbi Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells
    Panagiota A Sotiropoulou
    Cancer Immunology and Immunotherapy Center, Saint Savas Hospital, 171 Alexandras Avenue, 115 22, Athens, Greece
    Stem Cells 24:462-71. 2006
    ..In conclusion, in this report we define the optimal culture conditions for the successful isolation and expansion of human MSCs in high numbers for subsequent cellular therapeutic approaches...
  39. pmc Collective migration of an epithelial monolayer in response to a model wound
    M Poujade
    Laboratoire Physico Chimie Curie Unité Mixte de Recherche 168, Institut Curie, Centre National de Recherche Scientifique, Universite Pierre et Marie Curie, F 75248 Paris, France
    Proc Natl Acad Sci U S A 104:15988-93. 2007
    ..Experiments performed with autocrine cells constitutively expressing hepatocyte growth factor (HGF) or in the presence of exogenous HGF show a higher average velocity of the border and no leader...
  40. ncbi Clinical-grade production of human mesenchymal stromal cells: occurrence of aneuploidy without transformation
    Karin Tarte
    INSERM U917, Universite Rennes 1, Rennes, France
    Blood 115:1549-53. 2010
    ..However, MSCs with or without chromosomal alterations showed progressive growth arrest and entered senescence without evidence of transformation either in vitro or in vivo...
  41. ncbi Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods
    J van der Valk
    Fac Veterinary Medicine, Utrecht University, Yalelaan 2, 3584 CM Utrecht, The Netherlands
    Toxicol In Vitro 24:1053-63. 2010
    ..In this report, the results from the meeting are discussed and the formulation of a basic serum-free medium is suggested. Furthermore, recommendations are provided to improve information exchange on newly developed serum-free media...
  42. ncbi Stem cell properties of human dental pulp stem cells
    S Gronthos
    Craniofacial and Skeletal Diseases Branch, Building 30, Room 228, NIDCR, NIH, Bethesda, MD 20892, USA
    J Dent Res 81:531-5. 2002
    ..Taken together, these results demonstrate that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation...
  43. ncbi Cotransplantation of ex vivo expanded mesenchymal stem cells accelerates lymphocyte recovery and may reduce the risk of graft failure in haploidentical hematopoietic stem-cell transplantation
    Lynne M Ball
    Department of Pediatric Stem Cell Transplantation, Leiden University Medical Centre, Leiden, The Netherlands
    Blood 110:2764-7. 2007
    ..These data suggest that MSCs, possibly thanks to their potent immunosuppressive effect on alloreactive host T lymphocytes escaping the preparative regimen, reduce the risk of graft failure in haploidentical HSC transplant recipients...
  44. ncbi Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage
    Hirofumi Suemori
    Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 345:926-32. 2006
    ..These results provide valuable information that will assist in achieving the goal of the large-scale hES cell culture required for the application of hES cells to disease therapy...
  45. ncbi Cell culture: biology's new dimension
    Alison Abbott
    Nature 424:870-2. 2003
  46. ncbi Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells
    Jonathan S Draper
    Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Nat Biotechnol 22:53-4. 2004
    ..These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental...
  47. ncbi Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood
    Karen Bieback
    Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden Württemberg Hessen, Friedrich Ebert Strasse 107, D 68167 Mannheim
    Stem Cells 22:625-34. 2004
    ..Because MSC-like cells can be isolated at high efficacy from full-term UCB donations, we regard UCB as an additional stem cell source for experimental and potentially clinical purposes...
  48. pmc A functionally characterized test set of human induced pluripotent stem cells
    Gabriella L Boulting
    The Howard Hughes Medical Institute, Cambridge, Massachusetts, USA
    Nat Biotechnol 29:279-86. 2011
    ..This iPSC and ESC test set is a robust resource for those interested in the basic biology of stem cells and their applications...
  49. pmc A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential
    Gesine Kogler
    Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University Medical Center, Moorenstrasse 5, 40225 Dusseldorf, Germany
    J Exp Med 200:123-35. 2004
    ..No tumor formation was observed in any of these animals...
  50. ncbi Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages
    S I Nishikawa
    Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto, Japan
    Development 125:1747-57. 1998
    ..This line of evidence implicates Flk1+VE-cadherin+ cells as a diverging point of hemopoietic and endothelial cell lineages...
  51. pmc Small molecule mesengenic induction of human induced pluripotent stem cells to generate mesenchymal stem/stromal cells
    Yen Shun Chen
    The University of Queensland, UQ Centre for Clinical Research, Brisbane, Australia
    Stem Cells Transl Med 1:83-95. 2012
    ....
  52. ncbi Genomic alterations in cultured human embryonic stem cells
    Anirban Maitra
    McKusick Nathans Institute of Genetic Medicine, Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Genet 37:1099-103. 2005
    ....
  53. ncbi Recent advances in three-dimensional multicellular spheroid culture for biomedical research
    Ruei Zeng Lin
    Institute of Molecular Medicine, National Tsing Hua University, Hsin Chu, Taiwan
    Biotechnol J 3:1172-84. 2008
    ..Here we review the current understanding of multicellular spheroid formation mechanisms, their biomedical applications, and recent advances in spheroid culture, manipulation, and analysis techniques...
  54. ncbi Engineering tumors with 3D scaffolds
    Claudia Fischbach
    School of Engineering and Applied Sciences, Harvard University, 40 Oxford Street Cambridge, Massachusetts 02138, USA
    Nat Methods 4:855-60. 2007
    ..This new biomimetic model may provide a broadly applicable 3D culture system to study the effect of microenvironmental conditions on tumor malignancy in vitro and in vivo...
  55. ncbi Expansion of human adult stem cells from bone marrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality
    Ichiro Sekiya
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112 2699, USA
    Stem Cells 20:530-41. 2002
    ..The results indicate that, when preparing cultures of human MSCs, it is necessary to compromise between conditions that provide the highest overall yields and those that provide the highest content of early progenitor cells...
  56. pmc Butyrate greatly enhances derivation of human induced pluripotent stem cells by promoting epigenetic remodeling and the expression of pluripotency-associated genes
    Prashant Mali
    Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Stem Cells 28:713-20. 2010
    ..Moreover, butyrate stimulation provides an efficient method for reprogramming various human adult somatic cells, including cells from patients that are more refractory to reprogramming...
  57. ncbi Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells
    Gabsang Lee
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10021, USA
    Nat Biotechnol 25:1468-75. 2007
    ..The availability of unlimited numbers of human NCS cells offers new opportunities for studies of neural crest development and for efforts to model and treat neural crest-related disorders...
  58. ncbi Mesenchymal stem cells: clinical applications and biological characterization
    Frank P Barry
    Osiris Therapeutics Inc, 2001 Aliceanna Street, Baltimore, MD 21231, USA
    Int J Biochem Cell Biol 36:568-84. 2004
    ..These include the host immune response to implanted cells, the homing mechanisms that guide delivered cells to a site of injury and the differentiation in vivo of implanted cells under the influence of local signals...
  59. ncbi HOXB4-induced expansion of adult hematopoietic stem cells ex vivo
    Jennifer Antonchuk
    Terry Fox Laboratory, British Columbia Cancer Agency, 601 West 10th Avenue, V5Z 1L3, Vancouver, British Columbia, Canada
    Cell 109:39-45. 2002
    ..Importantly, these HSCs retained full lympho-myeloid repopulating potential and enhanced in vivo regenerative potential, demonstrating the feasibility of achieving significant ex vivo expansion of HSCs without functional impairment...
  60. ncbi Markers distinguishing mesenchymal stem cells from fibroblasts are downregulated with passaging
    Svetlana Halfon
    Teva Pharmaceutical Industries, Rehovot, Israel
    Stem Cells Dev 20:53-66. 2011
    ....
  61. ncbi Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential
    Gianluca D'Ippolito
    Geriatric Research, Education, and Clinical Center and Research Service, Veterans Affairs Medical Center and Department of Medicine, University of Miami School of Medicine, Miami, FL 33125, USA
    J Cell Sci 117:2971-81. 2004
    ..MIAMI cells proliferate extensively without evidence of senescence or loss of differentiation potential and thus may represent an ideal candidate for cellular therapies of inherited or degenerative diseases...
  62. ncbi Isolation and characterization of multipotent skin-derived precursors from human skin
    Jean G Toma
    Department of Developmental Biology, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
    Stem Cells 23:727-37. 2005
    ..Together, these data indicate that SKPs are an endogenous multipotent precursor cell present in human skin that can be isolated and expanded and differentiate into both neural and mesodermal cell types...
  63. ncbi High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array
    Yi Chung Tung
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA
    Analyst 136:473-8. 2011
    ..The multiplexed 3D hanging drop culture and testing plate provides an efficient way to obtain biological insights that are often lost in 2D platforms...
  64. pmc Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers
    Jérôme M Goffin
    Laboratory of Cell Biophysics, Ecole Polytechnique Federale de Lausanne, CH 1015, Lausanne, Switzerland
    J Cell Biol 172:259-68. 2006
    ..We propose that the different molecular composition and higher phosphorylation of FAs on supermature islets, compared with FAs on classical islets, accounts for higher stress resistance...
  65. ncbi A novel exposure system for the efficient and controlled deposition of aerosol particles onto cell cultures
    Melanie Savi
    Institute of Anatomy, University of Bern, Bern, Switzerland
    Environ Sci Technol 42:5667-74. 2008
    ..In situ analyses of the lung cells, e.g., the ciliary beat frequency, indicative of the defense capability of the cells, are complemented by off-line biochemical, physiological, and morphological cell analyses...
  66. pmc Aging of mesenchymal stem cell in vitro
    Mandana Mohyeddin Bonab
    Hematology Oncology and BMT Research Center, Shariati Hospital, Tehran University of Medical Sciences, Iran
    BMC Cell Biol 7:14. 2006
    ..The low frequency of this subpopulation of stem cells in bone marrow (BM) necessitates their in vitro expansion prior to clinical use. We evaluated the effect of long term culture on the senescence of these cells...
  67. ncbi Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells
    Miriam Bibel
    Novartis Institutes for BioMedical Research, Neuroscience Research, CH 4002 Basel, Switzerland
    Nat Protoc 2:1034-43. 2007
    ..At least 2 weeks are required from starting ES cell culture until plating progenitors and differentiating neurons establish synaptic transmission within about 10 days...
  68. ncbi Long term culture of human embryonic stem cells on recombinant vitronectin in ascorbate free media
    Andrew B J Prowse
    The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland 4072, Australia
    Biomaterials 31:8281-8. 2010
    ..The culture method outlined here provides a platform for future investigation into growth factor and extracellular matrix effects on hESC maintenance prior to bioreactor scale-up...
  69. pmc A microfluidic culture platform for CNS axonal injury, regeneration and transport
    Anne M Taylor
    Department of Biomedical Engineering, University of California, Irvine, 204 Rockwell Engineering, Irvine, California 92697, USA
    Nat Methods 2:599-605. 2005
    ..The results presented here demonstrate several experimental paradigms using the microfluidic platform, which can greatly facilitate future studies in axonal biology...
  70. ncbi Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow
    Karen Bieback
    Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden Württemberg Hessen, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Heidelberg, Germany
    Stem Cells 27:2331-41. 2009
    ..Telomerase activity was not detected in any of the cultures at any passage. In contrast to previous data from adipose tissue-derived MSCs, pHPL was found to be the most suitable FBS substitute in clinical scale BM-MSC expansion...
  71. ncbi Differentiation of human embryonic stem cells to neural lineages in adherent culture by blocking bone morphogenetic protein signaling
    Lesley Gerrard
    Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian, EH25 9PS, UK
    Stem Cells 23:1234-41. 2005
    ..Our findings will provide a platform for studying the molecular mechanism controlling neural differentiation...
  72. pmc Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells
    Ning Sun
    Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:15720-5. 2009
    ....
  73. pmc Substrate compliance versus ligand density in cell on gel responses
    Adam Engler
    School of Engineering and Applied Science, Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 86:617-28. 2004
    ..Based on such results, we hypothesize a central structural role for the cytoskeleton in driving the membrane outward during spreading whereas adhesion reinforces the spreading...
  74. ncbi Generation of pig induced pluripotent stem cells with a drug-inducible system
    Zhao Wu
    Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, People s Republic of China
    J Mol Cell Biol 1:46-54. 2009
    ..Moreover, the porcine iPS cells produced may be directly useful for the generation of precise gene-modified pigs...
  75. ncbi Efficient differentiation of hepatocytes from human embryonic stem cells exhibiting markers recapitulating liver development in vivo
    David C Hay
    Institute of Reproductive and Developmental Biology, Imperial College London, Du Cane Rd, London W12ONN, UK
    Stem Cells 26:894-902. 2008
    ..Our results have provided evidence that hESCs can be differentiated efficiently in vitro to functional hepatocytes, which may be useful as an in vitro system for toxicity screening in drug discovery...
  76. ncbi Transcriptional profiling of mucociliary differentiation in human airway epithelial cells
    Andrea J Ross
    Clinical Research Branch, Human Studies Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, University of North Carolina, Chapel Hill, North Carolina 27599 7315, USA
    Am J Respir Cell Mol Biol 37:169-85. 2007
    ..This work has provided extensive information about gene expression changes during differentiation of airway epithelial cells, and will be a useful resource for researchers interested in respiratory function, pathology, and toxicology...
  77. pmc Human cardiac stem cells
    Claudia Bearzi
    Department of Medicine, Cardiovascular Research Institute, New York Medical College, Valhalla, NY 10595, USA
    Proc Natl Acad Sci U S A 104:14068-73. 2007
    ..Thus, hCSCs can be isolated and expanded in vitro for subsequent autologous regeneration of dead myocardium in patients affected by heart failure of ischemic and nonischemic origin...
  78. ncbi Tissue engineering--current challenges and expanding opportunities
    Linda G Griffith
    Department of Chemical Engineering, Division of Bioengineering and Environmental Health, and Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 295:1009-14. 2002
    ....
  79. ncbi Parthenogenetic embryo-like structures in the human ovarian surface epithelium cell culture in postmenopausal women with no naturally present follicles and oocytes
    Irma Virant-Klun
    Department of Obstetrics and Gynecology, University Medical Centre, Ljubljana, Slovenia
    Stem Cells Dev 18:137-49. 2009
    ..In conclusion, the discovered cells expressed embryonic stem cell markers, gave rise to embryoid body-, oocyte-, and blastocyst-like structures, and might be involved in the human reproduction and formation of ovarian tumors...
  80. pmc Directed differentiation of human-induced pluripotent stem cells generates active motor neurons
    Saravanan Karumbayaram
    Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    Stem Cells 27:806-11. 2009
    ..These findings demonstrate the feasibility of using iPS-derived motor neuron progenitors and motor neurons in regenerative medicine applications and in vitro modeling of motor neuron diseases...
  81. ncbi Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection
    Melody Baddoo
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Cell Biochem 89:1235-49. 2003
    ..Therefore, FGF2 appears to function as a mitogen and self-maintenance factor for murine MSCs enriched from bone marrow by negative selection...
  82. ncbi Spheres derived from the human SK-RC-42 renal cell carcinoma cell line are enriched in cancer stem cells
    Yong Zhong
    Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China
    Cancer Lett 299:150-60. 2010
    ..The immunophenotype of the SFCs demonstrated in this study suggests that CSCs might play an important role in the evasion of tumor growth from immune surveillance...
  83. pmc Generation of induced pluripotent stem cell lines from Tibetan miniature pig
    Miguel A Esteban
    Stem Cell and Cancer Biology Group, Chinese Academy of Sciences Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou 510663, USA
    J Biol Chem 284:17634-40. 2009
    ..Because porcine physiology closely resembles human, the iPS cells reported here provide an attractive model to study certain human diseases or assess therapeutic applications of iPS in a large animal model...
  84. ncbi Ex vivo expansion of mesenchymal stromal cells
    Maria Ester Bernardo
    Dipartimento di Ematologia ed Oncologia Pediatrica, IRCCS Ospedale Pediatrico Bambino Gesù, P le Sant Onofrio, 4, 00165 Roma, Italy
    Best Pract Res Clin Haematol 24:73-81. 2011
    ..Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed...
  85. ncbi Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells
    Genta Narazaki
    Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Circulation 118:498-506. 2008
    ..However, detailed differentiation properties and the directional differentiation system of iPS cells have not been demonstrated...
  86. ncbi Puromycin-based purification of rat brain capillary endothelial cell cultures. Effect on the expression of blood-brain barrier-specific properties
    N Perriere
    CNRS UMR 7157, INSERM U705, University Paris 7, University Paris 5, Hopital Fernand Widal, Paris, France
    J Neurochem 93:279-89. 2005
    ..In conclusion, this method of RBEC purification will allow the production of in vitro models of the rat blood-brain barrier for cellular and molecular biology studies as well as pharmacological investigations...
  87. ncbi 3-D tumor model for in vitro evaluation of anticancer drugs
    Jayme L Horning
    Department of Pharmaceutical Sciences, College of Pharmacy, The University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    Mol Pharm 5:849-62. 2008
    ..A well-characterized 3-D model can particularly be useful for rapid screening of a large number of therapeutics for their efficacy during the drug discovery phase...
  88. pmc Derivation of midbrain dopamine neurons from human embryonic stem cells
    Anselme L Perrier
    Laboratory of Stem Cell and Tumor Biology, Division of Neurosurgery and Developmental Biology Program, and Cell Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:12543-8. 2004
    ..This experimental system also provides a powerful tool to probe the molecular mechanisms that control the development and function of human midbrain DA neurons...
  89. ncbi Role of oxidative damage in toxicity of particulates
    Peter Møller
    Department of Public Health, Section of Environment Health, University of Copenhagen, Copenhagen, Denmark
    Free Radic Res 44:1-46. 2010
    ..The results indicate that oxidative stress and elevated levels of oxidatively altered biomolecules are important intermediate endpoints that may be useful markers in hazard characterization of particulates...
  90. ncbi Complete meiosis from human induced pluripotent stem cells
    C Eguizabal
    Center for Regenerative Medicine in Barcelona, Barcelona, Spain
    Stem Cells 29:1186-95. 2011
    ..Our work brings us closer to the production of personalized human gametes in vitro...
  91. ncbi Osteoprogenitor response to semi-ordered and random nanotopographies
    Matthew John Dalby
    Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Biomaterials 27:2980-7. 2006
    ..The results demonstrated that the nanotopographies stimulated the osteoprogenitor cell differentiation towards an osteoblastic phenotype...
  92. ncbi Modeling morphogenesis and oncogenesis in three-dimensional breast epithelial cultures
    Christy Hebner
    Department of Surgery and Center for Bioengineering and Tissue Regeneration, University of California, San Francisco, CA, USA
    Annu Rev Pathol 3:313-39. 2008
    ..We review how 3D models are being used to investigate fundamental cellular and biophysical mechanisms associated with breast cancer progression that have not been readily amenable to traditional genetic or biochemical analysis...
  93. pmc Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds
    Shulamit Levenberg
    Department of Chemical Engineering, Divisions of Health Sciences and Technology, and Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139
    Proc Natl Acad Sci U S A 100:12741-6. 2003
    ..This approach provides a unique culture system for addressing questions in cell and developmental biology, and provides a potential mechanism for creating viable human tissue structures for therapeutic applications...
  94. ncbi Feeder layer- and serum-free culture of human embryonic stem cells
    M Amit
    Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, and the Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, 31096 Haifa, Israel
    Biol Reprod 70:837-45. 2004
    ..The feeder layer-free culture system reported here aims at facilitating research practices and providing a safer alternative for future clinical applications of hES cells...
  95. pmc Reproducible, ultra high-throughput formation of multicellular organization from single cell suspension-derived human embryonic stem cell aggregates
    Mark D Ungrin
    Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
    PLoS ONE 3:e1565. 2008
    ....
  96. pmc Human ESCs predisposition to karyotypic instability: Is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties?
    Puri Catalina
    Andalusian Stem Cell Bank University of Granada, Instituto de Investigación Biomédica, Granada, Spain
    Mol Cancer 7:76. 2008
    ..Here, we prompted to compare the chromosomal stability of three hESC lines maintained in the same laboratory using identical culture conditions and passaging methods...
  97. ncbi Naive and primed pluripotent states
    Jennifer Nichols
    Wellcome Trust Centre for Stem Cell Research, Department of Physiology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Cell Stem Cell 4:487-92. 2009
    ..We propose that two phases of pluripotency can be defined: naive and primed. This distinction extends to pluripotent stem cells derived from embryos or by molecular reprogramming ex vivo...
  98. pmc Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro
    Yunhan Hong
    Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    Proc Natl Acad Sci U S A 101:8011-6. 2004
    ..Our findings and cell line will offer a unique opportunity to study and recapitulate spermatogenesis in vitro and to develop approaches for germ-line transmission...
  99. ncbi Treatment of nasopharyngeal carcinoma with Epstein-Barr virus--specific T lymphocytes
    Karin C M Straathof
    Center for Cell and Gene Therapy, Department of Pediatrics, Baylor College of Medicine, 1102 Bates St, Suite 1120, Houston, TX 77030, USA
    Blood 105:1898-904. 2005
    ..These results demonstrate that administration of EBV-specific CTLs to patients with advanced NPC is feasible, appears to be safe, and can be associated with significant antitumor activity...
  100. ncbi Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells
    Mehdi Totonchi
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Int J Dev Biol 54:877-86. 2010
    ..Such hiPSCs could be useful particularly in the context of in vitro disease modeling, pharmaceutical screening and in cellular replacement therapies once the safety issues have been overcome...
  101. ncbi Metabolic flux analysis in mammalian cell culture
    Lake Ee Quek
    Australian Institute for Bioengineering and Nanotechnology AIBN, The University of Queensland, Brisbane QLD 4072, Australia
    Metab Eng 12:161-71. 2010
    ..Advances in both modeling and analytical techniques are bringing this challenge within sight...

Research Grants80

  1. Regulation of Apical Polarity in Breast Cancer
    SOPHIE LELIEVRE; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  2. System-level properties of the E. coli metabolic network
    ZOLTAN OLTVAI; Fiscal Year: 2006
    ..abstract_text> ..
  3. Drug Transporters and the Disposition of ADHD Therapeutic Agents
    John S Markowitz; Fiscal Year: 2010
    ....
  4. Development of tissue-specific cell lines in Drosophila
    Amanda Simcox; Fiscal Year: 2009
    ..Subsequent use of the cell lines by individual investigators to study diverse biological problems is expected to impact our understanding of human disease. ..
  5. MUTANT LINES OF ZEBRAFISH HIGHLY SENSITIVE TO NEOPLASIA
    Jan Spitsbergen; Fiscal Year: 2005
    ..These lines of zebrafish will provide an efficient and inexpensive system for development of new treatment and prevention strategies for cancer, and will help identify new genes controlling growth and differentiation. ..
  6. Nitric oxide in bladder neural-epithelial signaling
    Lori Birder; Fiscal Year: 2005
    ..Elucidation of mechanisms impacting on urothelial function may provide insights into the pathology of bladder dysfunction. ..
  7. CELL COMMUNICATION IN RETINA
    Shu Zhen Wang; Fiscal Year: 2005
    ..Once the functional glutamate receptors are characterized, double immunolabeling is used to confirm their presence on the dendrites of the ganglion cell where it receives excitation from dyad synapses. ..
  8. Inhibition of TNF in Patients with Alcoholic Hepatitis
    Vijay Shah; Fiscal Year: 2005
    ..abstract_text> ..
  9. Functional Genomics of Dictyostelium Development
    William Loomis; Fiscal Year: 2005
    ..Predictions derived from such networks will be tested when microarray analyses are extended to include further developmental mutants. ..
  10. Neuroprotective effect of HSP27 in Cerebral Ischemia
    Jun Chen; Fiscal Year: 2010
    ..The in vitro models will complement the in vivo studies by allowing for precise mechanistic studies to be performed. ..
  11. Programmed Cell Death Induced by Cerebral Ischemia
    Jun Chen; Fiscal Year: 2006
    ..abstract_text> ..
  12. Role of TRP Channels in Statin Reversal of Nephropathy in Early Stage Type 2 DM
    STEVEN SANSOM; Fiscal Year: 2009
    ..These studies will determine if and how a certain drug, simvastatin, can be used to treat patients in the early stage of type 2 diabetes mellitus and prevent or slow the progression of kidney disease. ..
  13. Molecular Mechanisms of Portal Hypertension
    Vijay Shah; Fiscal Year: 2007
    ..abstract_text> ..
  14. Regulation of TRAIL-induced apoptosis
    Francis Chan; Fiscal Year: 2009
    ..Our experiments may also provide a useful diagnostic tool to determine response of cancers to treatments involving TRAIL or agonist TRAIL antibodies. ..
  15. CCNY/MSKCC BIOMEDICAL ENGINEERING PARTNERSHIP
    JOHN TARBELL; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  16. Dermal-Epidermal Communication during Wound Healing
    Alan Wells; Fiscal Year: 2008
    ..These investigations will define key extracellular and intracellular molecules for wound resolution. These are potential targets for rationally designed interventions to promote normal wound healing and limit scarring. ..
  17. Apoptosis Execution Pathways in Dopaminergic Cell Death
    Jun Chen; Fiscal Year: 2007
    ..3. Test the hypotheses that Bax is a direct mediator for AIF release, and Bax contributes to dopaminergic cell death by activating both AIF-dependent and caspase-dependent pathways. ..
  18. Correlative Multimodal Imaging of Cardiac Electrophysiology and Metabolism
    JOHN PETER contact WIKSWO; Fiscal Year: 2010
    ..This work will be a key step towards developing improved metabolic therapies. ..
  19. Role of Programmed Necrosis in Immune Responses
    Francis Chan; Fiscal Year: 2006
    ..Moreover, controlling cell death may offer a novel approach to treating autoimmune diseases. [unreadable] [unreadable]..
  20. Small RNAs in Stem Cell Differentiation and Neural Development
    Robert H Blelloch; Fiscal Year: 2010
    ..Therefore, a systematic analysis of their function in embryonic and somatic stem cells as proposed in this grant should provide important insights into how they influence and can be used to diagnose and treat human disease. ..
  21. MECHANISMS OF CHLORPYRIFOS DEVELOPMENTAL NEUROTOXICITY
    Theodore Slotkin; Fiscal Year: 2004
    ..Molecular mechanisms of developmental neurotoxicity can be determined and linked to eventual alterations in behavioral performance. ..
  22. REG OF NITRIC OXIDE PRODUCT IN THE HEPATIC CIRCULATION
    Vijay Shah; Fiscal Year: 2002
    ..Defining the mechanisms that regulate nitric oxide production in SEC may lead to therapeutic options whereby NO activity may be pharmacologically or genetically modulated and benefit patients with cirrhosis and portal hypertension. ..
  23. INTEGRATION OF FIBROBLAST FUNCTIONING IN WOUND HEALING
    Alan Wells; Fiscal Year: 2003
    ..Understanding the delicate balance of these molecular mechanisms is important for rationally designing interventions to achieve normal wound healing. ..
  24. DRUGS AND DEVELOPMENT OF THE ADRENERGIC NERVOUS SYSTEM
    Theodore Slotkin; Fiscal Year: 2003
    ....
  25. WALL SHEAR STRESS IN THE CARDIOVASCULAR SYSTEM
    JOHN TARBELL; Fiscal Year: 2003
    ....
  26. DISSECTING ENZYMATIC NETWORKS IN SINGLE NEURONS
    Nancy Allbritton; Fiscal Year: 2003
    ..This information can be used to map the temporal interrelationships of neuronal signaling networks. As a result many fundamental questions regarding neuronal development, synaptic plasticity, brain injury, and aging can be answered. ..
  27. BLADDER CANCER BIOMARKERS GUIDES TO SURGERY AND THERAPY
    Robert Hurst; Fiscal Year: 2003
    ..The results of this study should generate biomarker profiles that are based in basic, mechanistic understanding of metastasis that can subsequently be tested in controlled clinical studies. ..
  28. MEASUREMENT OF KINASE ACTIVITIES IN SINGLE TUMOR CELLS
    Nancy Allbritton; Fiscal Year: 2001
    ..When fully developed, this integrated microchip will represent a new paradigm in single-cell measurement techniques. ..
  29. SHEAR STRESS EFFECTS ON ENDOTHELIAL TRANSPORT
    John M Tarbell; Fiscal Year: 2010
    ..abstract_text> ..
  30. SIGNAL TRANSDUCTION THROUGH CALCIUM WAVES
    Nancy Allbritton; Fiscal Year: 2003
    ....
  31. Toward Tissue Engineering of the Knee Meniscus
    Kyriacos A Athanasiou; Fiscal Year: 2010
    ..Successful completion of this proposal will establish a framework for future scaffoldless meniscus regeneration attempts using alternate cell sources for the repair and replacement of meniscus tissue. ..
  32. Urothelial cell response to bacterial infection
    Robert Hurst; Fiscal Year: 2004
    ..These data will form an essential element in either an R01 or a program project and will permit the PI to develop a new line of urologic research. ..
  33. SIGNAL TRANSDUCTION MECHANISMS IN EPIDERMIS
    Miroslav Blumenberg; Fiscal Year: 2003
    ..abstract_text> ..
  34. LASER DOPPLER VELOCIMETER SYSTEM FOR BIOMEDICAL STUDIES
    JOHN TARBELL; Fiscal Year: 2001
    ..The system will be the primary measurement system for the ongoing research in cardiovascular prosthetics including mechanical heart valve cavitation and ventricular assist devices. ..
  35. Dietary Cysteine:Conceptal Response to Oxidative Stress
    Craig Harris; Fiscal Year: 2002
    ....
  36. Rapid Analysis of Signaling in Adherent Cells
    Nancy Allbritton; Fiscal Year: 2008
    ....
  37. Micropallet Arrays for Separation of Single Cells and Colonies
    Nancy L Allbritton; Fiscal Year: 2010
    ..The applications which involve basic cell biology, tumorigenesis, and signaling mechanisms in diabetes mellitus will demonstrate that the novel separation system should be widely applicable to the biomedical sciences. ..
  38. An Automated Platform for Kinase Assays on Patient Cells
    Nancy Allbritton; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  39. Endothelial Nitric Oxide Synthase & Portal Hypertension
    Vijay Shah; Fiscal Year: 2002
    ..abstract_text> ..
  40. Intercellular signaling during terminal differentiation
    William Loomis; Fiscal Year: 2009
    ..Together these studies will add to the foundation for improvements in the diagnosis and treatment of neurological diseases. ..
  41. Mechanochemical regulation of endothelial permeability
    Konstantin Birukov; Fiscal Year: 2007
    ....
  42. Synthesis and Characterization of Patterned Microvascular Networks
    Joe Tien; Fiscal Year: 2009
    ..In the long-term, engineered networks may enhance the perfusion of artificial tissues in vitro, and thereby overcome one of the primary obstacles in tissue engineering. ..
  43. Etiology of Pediatric Atopy
    Edward Zoratti; Fiscal Year: 2008
    ..Blood specimens, DNA and mRNA from parents and subjects will be placed in a biorepository for future molecular epidemiology studies. ..
  44. Pre-association and Signaling by TNF-like Receptors
    Francis Chan; Fiscal Year: 2003
    ..The second part of this proposal attempts to elucidate the mechanism by which TNFR coordinates the recruitment of these signaling proteins. ..
  45. Failure of FasL Mediated Pathways in Tumor Angiogenesis
    Vijay Shah; Fiscal Year: 2006
    ..These insights may lead to novel and effective therapeutic strategies that target this pathway to prevent angiogenesis, and thereby prevent colorectal tumor development growth and spread. ..
  46. b-catenin's role in establishing the gut stem cell niche
    Melissa Wong; Fiscal Year: 2009
    ..These proposed studies will directly examine the role of Wnt/beta-catenin signaling on maintenance of the stem cell niche and will shed insight into the regulatory control of intestinal stem cells. ..
  47. In search of the intestinal stem cell
    Melissa Wong; Fiscal Year: 2004
    ..These studies will determine if a signaling cascade is responsible for defining "stemness" or if a yet to be defined master regulator molecule directs synergy of the multiple pathways. ..
  48. MECHANISM OF REGULATION OF A GLOMERULAR K CHANNEL
    STEVEN SANSOM; Fiscal Year: 2005
    ..Ultimately, better management of some forms of hypertension could develop from an in-depth understanding of BK regulatory mechanism at the molecular level. ..
  49. In Vitro Synthesis of a Microvascular Network
    Joe Tien; Fiscal Year: 2004
    ..This work will further determine the thickness of tissue that can be sustained metabolically by an engineered microvascular construct comprised of two interconnected plexuses. ..
  50. Profiling Ras Activated Signal Transduction Pathways
    Nancy Allbritton; Fiscal Year: 2004
    ..The successful completion of this work will provide a new and powerful tool for basic research, drug discovery and screening, cancer classification, and potentially clinical decision making. ..
  51. Transplanted Stem Cells and Tumor Progression
    Melissa H Wong; Fiscal Year: 2010
    ..It is feasible that fusion promoting factors, or the fusion products themselves, may be useful targets for preventing tumor progression. ..
  52. Cell Cycle Mechanisms of PTH/PTHrP Action in Osteoblasts
    NABANITA DATTA; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  53. Self-Assembly of Mesostructure Biomaterials
    Joe Tien; Fiscal Year: 2004
    ..abstract_text> ..
  54. Genes & Proteins of Urothelial-ECM Interaction
    Robert Hurst; Fiscal Year: 2009
    ..The "deliverables" will be sets of genes and proteins subjected to several levels of verification for their role in aiding studies of epithelial-stromal interactions in normal and malignant tissues. ..
  55. Cytokine regulation of glial reactivity
    Gareth John; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  56. Analysis of COX-2 as a therapeutic target in acquired epilepsy (epileptogenesis)
    JAMES HEWETT; Fiscal Year: 2008
    ..Although there are numerous drugs available to effectively treat established epilepsy (i.e. anti-epileptic drugs), drugs that prevent epilepsy development (i.e. anti-epileptogenic drugs) remain to be identified. [unreadable] [unreadable]..
  57. MOLECULAR NETWORKS THAT COORDINATE DIFFERENTIATION
    William Loomis; Fiscal Year: 2003
    ..Together these studies will help to establish a signal transduction pathway that can serve as a model for studies in other systems. ..
  58. Fetal & Adolescent Nicotine Effects on CNS 5HT Systems
    Theodore Slotkin; Fiscal Year: 2003
    ..abstract_text> ..
  59. Regulation of Neuronal Growth Cone Guidance
    Daniel Suter; Fiscal Year: 2009
    ..Thus, they will have an impact on our understanding of axon guidance and nerve regeneration, as well as of tumor cell metastasis, another motile process, in which Src has been implicated. ..
  60. Soft Tissue Biomechanical Behavior During Acupuncture in Low Back Pain
    Helene M Langevin; Fiscal Year: 2010
    ....
  61. Microfluidic Tissue Engineering of Small Airway Injuries
    Shuichi Takayama; Fiscal Year: 2010
    ..Also, evaluate the effect of surfactant as a countermeasure to reduce cellular injuries. ..
  62. BIOMECHANICAL EFFECT OF ACUPUNCTURE NEEDLING
    HELENE LANGEVIN; Fiscal Year: 2001
    ..This will contribute to the fundamental understanding of acupuncture therapy and may lead to improvements in treatments. ..
  63. ECM Regulation by Estrogen in ARMD
    Sharon Elliot; Fiscal Year: 2006
    ..Conversely, we expect that estrogen's antioxidant action on the cell membrane will diminish MPO-induced blebbing and that activation of estrogen receptors will modify matrix molecule dysregulation. ..
  64. Control of Somatic Stem Cell Proliferation by Oct4
    Christopher Lengner; Fiscal Year: 2008
    ..This proposal will delineate molecular mechanisms that can potentially be manipulated to enhance regenerative medicine. [unreadable] [unreadable]..
  65. Cardiac Inflammation in Resuscitated Hemorrhagic Shock
    Douglas Mann; Fiscal Year: 2008
    ..Taken together, Specific Aims 1-4 should provide definitive new information regarding the mechanisms of activation, as well as the role of the innate immune system in resuscitated hemorrhagic shock. ..
  66. Training in Biomolecular and Tissue Engineering
    Farshid Guilak; Fiscal Year: 2007
    ..We will also take advantage of several long-standing and successful initiatives at Duke University to enhance the recruitment of underrepresented minorities as part of this post-doctoral training program. ..
  67. 3D Culture of mES Cells in Four-Compartment Bioreactors
    JOERG GERLACH; Fiscal Year: 2006
    ..abstract_text> ..
  68. Magnetic quantum dots for active sensing of the nuclear envelope
    Denis Wirtz; Fiscal Year: 2008
    ..3d. We will investigate whether and how the actin and microtubule networks affect the mechanical properties of the NE. [unreadable] [unreadable] [unreadable]..
  69. 2008 Musculoskeletal Biology and Bioengineering Gordon Research Conference
    Farshid Guilak; Fiscal Year: 2008
    ..The meeting will consist of 9 separate sessions, consisting of invited speakers and selected attendees at various levels of career development. [unreadable] [unreadable] [unreadable] [unreadable]..
  70. 3D Woven Scaffolds for Tissue Engineering
    Farshid Guilak; Fiscal Year: 2003
    ..An improved level of biomechanical function will increase the level of success in the engineered repair of various tissues of the musculoskeletal system as well as other organ systems of the body. ..
  71. Myocardial Regeneration with Adult Stem Cells
    Young Sup Yoon; Fiscal Year: 2009
    ....
  72. Role of Angiogenesis and Endothelial Progenitor Cells in Diabetic Heart Failure
    Young Sup Yoon; Fiscal Year: 2010
    ..abstract_text> ..
  73. Porous Silicon Particles for Oral Drug Delivery
    Peter Swaan; Fiscal Year: 2007
    ..This may allow for the delivery of multiple therapeutic macromolecules in a more controlled and targeted manner to the GI tract. [unreadable] [unreadable]..
  74. High-Throughput Assay for the Intestinal Peptide Transporter
    Peter Swaan; Fiscal Year: 2005
    ..This will aid in our understanding of the structural features that govern peptide and drug transport via this system and will significantly support studies aiming to target this transporter. ..
  75. Epithelial Transport and Function of Riboflavin
    Peter Swaan; Fiscal Year: 2003
    ..These studies will yield new and important information regarding a complex epithelial transport mechanism and provide important new insights in its structural specificity and function. ..
  76. LIPID/LIPOPROTEIN METABOLISM AND CARDIOVASCULAR DISEASES
    KAFAIT MALIK; Fiscal Year: 2007
    ..It is also our intention to provide postdoctoral trainees some experience in grant writing. ..
  77. Role of adenosine A3 receptor in suppressing prostate cancer
    Vickram Ramkumar; Fiscal Year: 2008
    ..Therefore, these goals will form the specific aims of the current grant proposal. We believe that this study could uncover a novel method of treating prostate cancer metastasis. [unreadable] [unreadable] [unreadable]..
  78. Regenerative Healing Using ECM Based Scaffolds
    Ioannis Yannas; Fiscal Year: 2009
    ..An attempt will be made to elucidate the mechanism of contraction blocking during healing of injured nerves, and to find out an association, if any, with induced regeneration. ..