Genomes and Genes
Summary: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or EMBRYO in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Publications241 found, 100 shown here
- Effects of beta-mercaptoethanol on formation of pronuclei and developmental competence of swamp buffalo oocytesN Songsasen
Department of Biological Science, University of New Orleans, New Orleans, LA 70131, USA
Anim Reprod Sci 71:193-202. 2002..This study provides information for further investigations on optimizing a system for in vitro production of swamp buffalo embryos...
- Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor besciiJoel Farkas
Department of Genetics, University of Georgia, Athens, GA 30602, USA
J Ind Microbiol Biotechnol 40:41-9. 2013..We report new growth media and culture techniques for Caldicellulosiruptor bescii, the most thermophilic cellulolytic bacterium known...
- Notch signaling regulates the pattern of auditory hair cell differentiation in mammalsA Zine
Institute of Physiology, University of Lausanne, Switzerland
Development 127:3373-83. 2000..Our data suggest that the Notch1 signaling pathway is involved in a complex interplay between the consequences of different ligand-Notch1 combinations during cochlear morphogenesis and the phases of hair cell differentiation...
- Association between dental pulp stones and calcifying nanoparticlesJinfeng Zeng
Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology and Institute of Stomatological Research, Sun Yat Sen University, Guangzhou, China
Int J Nanomedicine 6:109-18. 2011..CNPs could be detected and isolated from a high percentage of dental pulp stones, suggesting that CNPs might play an important role in the calcification of dental pulp...
- Transplantation of cultured bone cells using combinations of scaffolds and culture techniquesToshimasa Uemura
Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, Tsukuba, Ibaraki 305 8562, Japan
Biomaterials 24:2277-86. 2003..this therapy, there remain several problems to be overcome, for example, the improvements of scaffolds and culture techniques. In this review article, two kinds of porous ceramics, a novel sintered porous hydroxyapatite and a porous ..
- A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat cultureHolger Janssen
Institut für Biowissenschaften, Universitat Rostock, Germany
Appl Microbiol Biotechnol 87:2209-26. 2010..Thus, a first systematic study of acidogenic and solventogenic chemostat cultures is presented, and similarities as well as differences to previous studies of batch cultures are discussed...
- Evaluation of a galactose-carrying gelatin sponge for hepatocytes culture and transplantationSung Ran Hong
School of Chemical Engineering, College of Engineering, Hanyang University, Seungdong ku, Seoul, 133 791, Korea
J Biomed Mater Res A 67:733-41. 2003..Hepatic specific metabolic functions, namely, the secretion of serum albumin and the synthesis of urea, were well maintained and promoted by spheroidal hepatocytes formed in the MGLA sponge...
- Cinnamic-derived acids significantly affect Fusarium graminearum growth and in vitro synthesis of type B trichothecenesNadia Ponts
Department of Cell Biology and Neuroscience, University of California Riverside, CA, USA
Phytopathology 101:929-34. 2011..These data suggest that, in planta, different compositions in phenolic acids of kernels from various cultivars may reflect different degrees of sensitivity to "mycotoxinogenesis."..
- Changes in var gene mRNA levels during erythrocytic development in two phenotypically distinct Plasmodium falciparum parasitesMadeleine Dahlbäck
Centre for Medical Parasitology at Department of International Health, Immunology and Microbiology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark
Malar J 6:78. 2007..Two parasite populations were studied; an unselected population of NF54 and a selected population, NF54VAR2CSA, to compare both the transcription of var2csa and the expression pattern of the corresponding protein...
- Engineering micropatterned surfaces for the coculture of hepatocytes and Kupffer cellsYekaterina S Zinchenko
Mechanical Engineering Department, University of North Carolina at Charlotte, 9201 University City Blvd, Charlotte, NC 28223, USA
J Biomed Mater Res A 75:242-8. 2005..To demonstrate the success of the method, surface characterization of the resultant micropatterns, as well as morphological and functional results are also presented...
- Detection and isolation of nanobacteria-like particles from urinary stones: long-withheld dataHiromi Kumon
Department of Urology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
Int J Urol 18:458-65. 2011..To report our experimental results on detection and isolation of nanobacteria-like particles (NLP) from urinary stone samples...
- Effect of in vitro and in vivo culture on embryo development from prepubertal goat IVM-IVF oocytesD Izquierdo
Department de Ciència Animal i dels Aliments, Universitat Autonoma de Barcelona, Spain
Theriogenology 57:1431-41. 2002....
- Molecular evidence of false-positive cultures for Mycobacterium tuberculosis in a Taiwanese hospital with a high incidence of TBChih Cheng Lai
Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, No 7 Chun Shan South Rd, Taipei 100, Taiwan
Chest 137:1065-70. 2010..Isolation of Mycobacterium tuberculosis (MTB) from the clinical specimens of patients with suspected TB remains the gold standard for diagnosis of TB. However, false-positive MTB cultures can occur as a result of laboratory contamination...
- Attempts at in vitro fertilization and culture of in vitro matured oocytes in sei ( Balaenoptera borealis) and Bryde's ( B. edeni) whalesM M U Bhuiyan
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080 8555 Hokkaido, Japan
Zygote 17:19-28. 2009..1%) in sei whales. In conclusion, the present study indicates that IVF in sei whales is possible to achieve cleaved embryos developing to morula stage. This is the first in vitro embryo production attempt in sei and Bryde's whales...
- Identification and characterisation of mRif1: a mouse telomere-associated protein highly expressed in germ cells and embryo-derived pluripotent stem cellsIan R Adams
Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, Cambridge, United Kingdom
Dev Dyn 229:733-44. 2004..mRif1 may be involved in the maintenance of telomere length or pluripotency in the germline and during early mouse development...
- Comparison of nucleic acid amplification tests and culture techniques in the detection of Neisseria gonorrhoeae and Chlamydia trachomatis in victims of suspected child sexual abuseNancy D Kellogg
Department of Pediatrics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
J Pediatr Adolesc Gynecol 17:331-9. 2004..2) To compare Ligase Chain Reaction (LCR), Polymerase Chain Reaction (PCR), and culture methods in the detection of chlamydia and gonorrhea infection among prepubertal and adolescent girls referred for sexual abuse evaluations...
- Effect of donor stimulation, frozen semen and heparin treatment on the efficiency of in vitro embryo production in goatsLucyna Katska-Ksiazkiewicz
Department of Animal Reproduction, National Research Institute of Animal Production, 32 083 Balice Krakow, Poland
Theriogenology 62:576-86. 2004..Overall, more than 60% of the recipients became pregnant and 20% of all transferred embryos survived delivering 13 healthy kids. Our caprine IVP system allows obtaining embryos with developmental competence comparable to bovine IVP...
- Cultivation of explants of the marine sponge Crambe crambe in closed systemsEl Hassan Belarbi
Chemical Engineering Department, University of Almeria, 04071 Almeria, Spain
Biomol Eng 20:333-7. 2003..The sponges grew better in CFTHS compared with the DFTHS. The high growth rates observed in CFTHS suggest that this technique is a promising method for culturing C. crambe in closed systems...
- Biotechnological aspects of marine spongesRonald Osinga
J Biotechnol 100:91-2. 2003
- Apoptosis in the preimplantation mouse embryo: effect of strain difference and in vitro cultureMarziel Kamjoo
School of Biological Sciences, University of Manchester, Stopford Building, Oxford Rd, Manchester, United Kingdom
Mol Reprod Dev 61:67-77. 2002..0005 M16; P = 0.002 kSOM) and than C57BL6/CBA x C57BL6/CBA in M16 (P = 0.019). Our data suggest that genetic make-up and the chemical composition of simple medium are equally important in determining the level of apoptosis...
- Heart-valve mesenchyme formation is dependent on hyaluronan-augmented activation of ErbB2-ErbB3 receptorsTodd D Camenisch
College of Pharmacy and Steele Memorial Children s Research Center, University of Arizona, Tucson, Arizona, USA
Nat Med 8:850-5. 2002..These data provide a link between extracellular matrix-hyaluronan and ErbB receptor activation during development of early heart-valve and septal mesenchyme...
- Embryonic mouse submandibular salivary gland morphogenesis and the TNF/TNF-R1 signal transduction pathwayM Melnick
Laboratory for Developmental Genetics, University of Southern California, Los Angeles, California 90089 0641, USA
Anat Rec 262:318-30. 2001..The results of these experiments indicate that the TNF/TNF-R1 signal transduction pathway plays an important role in balancing cell proliferation and apoptosis during SMG duct and presumptive acini formation...
- Automated synchronization of Plasmodium falciparum parasites by culture in a temperature-cycling incubatorJ David Haynes
Department of Immunology, Walter Reed Army Institute of Research, Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
Methods Mol Med 72:489-97. 2002
- CUC2 as an early marker for regeneration competence in Arabidopsis root explantsHans Motte
Department of Plant Production, University College Ghent, member of the Ghent University Association, Schoonmeersstraat, 52, BE 9000 Ghent, Belgium
J Plant Physiol 168:1598-601. 2011..Thus, CUC2 is a predictive marker for the acquisition of root explant competence for root and shoot organogenesis...
- Hyperglycemia-induced vasculopathy in the murine vitelline vasculature: correlation with PECAM-1/CD31 tyrosine phosphorylation stateE Pinter
Departments of Pathology and Pediatrics, Yale University, Alexion Pharmaceuticals, Inc, New Haven, Connecticut, USA
Am J Pathol 154:1367-79. 1999....
- Association between self-replicating calcifying nanoparticles and aortic stenosis: a possible link to valve calcificationMiguel A Bratos-Pérez
Servicio de Microbiologia, Facultad de Medicina, Hospital Clinico Universitario, Valladolid, Spain
Eur Heart J 29:371-6. 2008..These CNPs have been detected and isolated from calcified human tissues, including blood vessels and kidney stones, and are referred to as nanobacteria. We evaluated calcific aortic valves for the presence of CNP...
- Developmental events and shoot apical meristem gene expression patterns during shoot development in Arabidopsis thalianaAndrew J Cary
Boyce Thompson Institute and Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA
Plant J 32:867-77. 2002..In contrast, the expression pattern of the enhancer trap lines during zygotic embryogenesis was more localized to the presumptive SAM even in early stages of embryogenesis...
- Direct DNA delivery into zebrafish embryos employing tissue culture techniquesR Sussman
Marine Biological Laboratory, Marine Resources Center, Woods Hole, Massachusetts 02543, USA
Genesis 31:1-5. 2001..These results demonstrate the ease of obtaining transfected fish embryos, which facilitate the mass production of new genotypes and extend the procedure to laboratories with limited resources...
- Theileria annulata: in vitro cultivation of schizont-infected bovine lymphocytesV Shkap
Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Vet Parasitol 65:11-20. 1996..In Roller bottles, with the same type and volume of medium and cultivation for 7 days without medium change, best yield resulted from seeding with the highest inoculum size of 4 x 10(7) cells per vessel...
- Nuclear translocation of nuclear factor kappa B in early 1-cell mouse embryosA Nishikimi
Laboratory of Reproductive Physiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
Biol Reprod 60:1536-41. 1999..Taken together, the results suggest that the activation of NF-kappaB at the early 1-cell stage is required for the development of mouse embryos beyond the 2-cell stage...
- Insulin and insulin-like growth factor-I promote rabbit blastocyst development and prevent apoptosisA Herrler
Department of Anatomy and Reproductive Biology, RWTH University of Aachen, 52057 Aachen, Germany
Biol Reprod 59:1302-10. 1998..By immunohistochemistry we could show that addition of insulin (0.68-68 nM) decreased apoptosis and increased cell proliferation in a dose-dependent manner, supporting blastocyst development significantly...
- Effects of protein source and energy substrates on the in vitro development of bovine embryos in a two-step culture systemKwang Taek Lim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
J Vet Sci 4:73-8. 2003..Therefore, the avoidance of this negative effect should allow more optimal conditions to be developed for in vitro production...
- Effects of donor oocytes and culture conditions on development of cloned mice embryosShaorong Gao
Department of Gene Expression and Development, The Roslin Institute, Roslin, Scotland, United Kingdom
Mol Reprod Dev 66:126-33. 2003..M16 medium is superior for culture of nuclear transfer embryos and low oxygen concentration with CZB medium during culture shows no benefit on development of cloned embryos...
- Double vitrification of rat embryos at different developmental stages using an identical protocolVladimir Isachenko
Department of Animal Production, Center for Agricultural Researches, P O Box 727, Zaragoza 50080, Spain
Theriogenology 60:445-52. 2003..The low survival rate of blastocysts subjected to double vitrification requires further investigation...
- Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitroS E Park
College of Medicine, Pochon CHA University and Infertility Medical Center of CHA General Hospital, Seoul, South Korea
Fertil Steril 76:167-74. 2001..CONCLUSION(S): A modified P-1 medium was developed, and it supported the development of both in vivo- and in vitro-derived ICR mouse embryos...
- Effect of leukemia inhibitory factor on bovine embryos produced in vitro under chemically defined conditionsS Sirisathien
Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602 7389, USA
Theriogenology 59:1751-63. 2003..Data indicate that addition of LIF has no major beneficial effect on bovine embryos produced in these chemically defined conditions...
- Effects of growth hormone and insulin-like growth factor-I on development of in vitro derived bovine embryosF Moreira
Department of Aninial Sciences, University of Florida, Gainesville 32611, USA
Theriogenology 57:895-907. 2002..In conclusion, both GH and IGF-I stimulate embryonic development in cattle and GH effects may likely involve IGF-I-independent mechanisms...
- Prevalence of apoptosis and inner cell allocation in bovine embryos cultured under different oxygen tensions with or without cysteine additionA Van Soom
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
Theriogenology 57:1453-65. 2002..Embryos cultured under 5% O2 in the presence of cysteine, however, possessed significantly higher numbers of ICM cells. This finding corroborates the theoretical assumption that antioxidants are beneficial for ICM development...
- Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative studyA van Langendonckt
Department of Gynecology, Catholic University of Louvain, Brussels, Belgium
Fertil Steril 76:1023-31. 2001..1% and 36.1%, respectively). CONCLUSION(S): In our day 3 embryo transfer program, G1.2/G2.2 media were superior to Sydney IVF media, whereas both media yielded similar outcomes in our blastocyst transfer program...
- Effects of fetuin on zona pellucida hardening, fertilization and embryo development in cattleF C Landim-Alvarenga
Department of Animal Reproduction and Veterinary Radiology, F M V Z UNESP, Botucatu, SP, Brazil
Anim Reprod Sci 71:181-91. 2002..Apparently fetuin can be used as a substitute for FCS during bovine oocyte maturation, since it leads to similar developmental rates as FCS in intact and partially stripped oocytes...
- Beta-alanine but not taurine can function as an organic osmolyte in preimplantation mouse embryos cultured from fertilized eggsMary Anne Hammer
Ottawa Health Research Institute Hormones, Growth and Development Program, Ontario
Mol Reprod Dev 66:153-61. 2003..Thus, the beta-amino acid transporter is likely present in early preimplantation embryos to supply beta-amino acids such as taurine for purposes other than to serve as organic osmolytes...
- In vitro production of embryos in swineL R Abeydeera
PIC International, Berkeley, CA 94710, USA
Theriogenology 57:256-73. 2002..Further research efforts should be directed to improve oocyte/embryo quality and the methods to minimize polyspermy through development of novel IVM, IVF, and EC techniques...
- Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and qualityPaloma Duque
Area de Genética y Reproducción, SERIDA, Centro de Fertilización in vitro de Asturias, C Alvarez Garaya 12, 33206, Gijon, Spain
Reprod Nutr Dev 43:487-96. 2003..Both Milli-Q and Sigma supported embryo development at comparable rates, although in the presence of BSA, blastocysts obtained in the medium prepared with Milli-Q water had superior quality in terms of ICM/total cells rates...
- Influences of epidermal growth factor and insulin-like growth factor-I on bovine blastocyst development in vitroS Sirisathien
Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602 7389, USA
Anim Reprod Sci 77:21-32. 2003..In conclusion, both EGF and IGF-I could independently enhance bovine preimplantational development in chemically defined media and IGF-I but not EGF may play a mitogenic role during early bovine development...
- Amino acid metabolism of preimplantation bovine embryos cultured with bovine serum albumin or polyvinyl alcoholNicolas M Orsi
Department of Biology, The University of York, PO Box 373, York YO10 5YW, UK
Theriogenology 61:561-72. 2004..These differences could partly be due to an increase in free intracellular amino acid concentration in SOFaaBSA embryos derived from hydrolysis of endocytosed BSA, and argue against the inclusion of PVA in bovine embryo culture media...
- Effect of protein supplementation on development to the hatching and hatched blastocyst stages of cat IVF embryosN W Kurniani Karja
Department of Veterinary Sciences, Yamaguchi University, Japan
Reprod Fertil Dev 14:291-6. 2002..The results of the present study indicate that supplementation with FBS at different stages of early embryo development promotes development to the hatching and hatched blastocyst stages of cat IVF embryos...
- A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst developmentN S Macklon
Division of Reproductive Medicine, Department of Obstetrics and Gynaecology, Rotterdam, The Netherlands
Hum Reprod 17:2700-5. 2002..However, their improved efficacy over monoculture systems remains to be demonstrated in prospective studies...
- Effects of thioredoxin on the preimplantation development of bovine embryosY Z Bing
National Agricultural Research Center for Tohoku Region, Morioka, Japan
Theriogenology 59:863-73. 2003....
- Use of two replacements of serum during bovine embryo culture in vitroPaloma Duque
Consejería de Medio Rural y Pesca, SERIDA CENSYRA, Camino de los Claveles 604, Somio, 33203 Gijón, Asturias, Spain
Theriogenology 59:889-99. 2003..The concentration of supplement and the timing of its inclusion in culture markedly affected embryo development. The serum replacement CPSR-3 can supplement embryo culture with blastocyst rates and quality similar to those for serum...
- Effect of the volume of medium and number of oocytes during in vitro fertilization on embryo development in pigsMaria Antonia Gil
Department of Animal Pathology, Veterinary Science, University of Murcia, Murcia E 30071, Spain
Theriogenology 60:767-76. 2003..These results indicate that when 2000 spermatozoa/oocyte were used, a low volume of IVF medium (0.1 ml) and the number of oocytes during IVF (30-50) can improve the in vitro embryo production in pigs...
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) acts independently of the beta common subunit of the GM-CSF receptor to prevent inner cell mass apoptosis in human embryosCecilia Sjoblom
Fertilitetscentrum AB, 402 29 Göteborg, Sweden
Biol Reprod 67:1817-23. 2002..Together, these data indicate that GM-CSF regulates cell viability in human embryos and that this potentially occurs through a novel receptor mechanism that is independent of betac...
- New developments reproductive technologies in deerD K Berg
Reproductive Technologies Group, AgResearch, Ltd, Ruakura Research Center, Private Bag 3123, Hamilton, New Zealand
Theriogenology 59:189-205. 2003..The process of developing an in vitro embryo production system for farmed red deer may serve as a beneficial model for the propagation of endangered cervine species...
- Optimization of culture medium for cloned bovine embryos and its influence on pregnancy and delivery outcomeY H Choi
Department of Theriogenology, College of Veterinary Medicine, Seoul, South Korea
Theriogenology 58:1187-97. 2002..However, enhanced development in vitro might not directly contribute to improving pregnancy outcomes...
- Use of G1.2/G2.2 media for commercial bovine embryo culture: equivalent development and pregnancy rates compared to co-cultureMichelle Lane
Research and Development Department, Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, CO 80110, USA
Theriogenology 60:407-19. 2003..2/G2.2 could be used in a commercial OPU transfer program without any loss in the numbers of blastocysts produced or the numbers of pregnancies resulting following transfer from either fresh or cryopreserved blastocysts...
- The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinxChin Der Chen
Department of Obstetrics and Gynecology, National Taiwan University College of Medicine and Hospital, Taipei, Taiwan
Hum Reprod 17:128-33. 2002....
- Vascular endothelial growth factor (VEGF) promotes the early development of bovine embryo in the presence of cumulus cellsHailing Luo
Department of Animal Production, College of Animal Science and Technology, China Agricultural University, Beijing, China
J Vet Med Sci 64:967-71. 2002..05) in all groups. These results suggest that VEGF has a beneficial effect on the initial development of bovine embryo through surrounding cumulus cells...
- Promoting effect of beta-mercaptoethanol on in vitro development under oxidative stress and cystine uptake of bovine embryosM Takahashi
Department of Animal and Grassland Research, National Agricultural Center for Kyushu Okinawa Region, Kumamoto 861 1192, Japan
Biol Reprod 66:562-7. 2002..These results indicate that beta-ME has a protective effect in embryo development against oxidative stress and that the effect of beta-ME is associated with the promotion of cystine uptake of low availability in embryos...
- Dynamic in vivo imaging of postimplantation mammalian embryos using whole embryo cultureE A V Jones
Department of Chemical Engineering, California Institute of Technology, Pasadena, California
Genesis 34:228-35. 2002..To highlight some of the strengths of this approach, we present time-lapse movies of embryonic turning, somite addition, closure of the neural tube, and fluorescent imaging of blood circulation in the yolk sac and embryo...
- Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesisRi Cheng Chian
Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada H3A 1A1
Reprod Biomed Online 4:127-32. 2002..9%, 79.9% and 73.9%) and blastocyst formation (27.4%, 33.0% and 30.4%) in the three groups. These results indicate that the maturational and developmental competence of immature oocytes is not affected by the phase of folliculogenesis...
- IGF-1/IGFBP-1 increases blastocyst formation and total blastocyst cell number in mouse embryo culture and facilitates the establishment of a stem-cell lineTa Chin Lin
Department of Gynecology, Obstetrics, and Infertility, Kuo General Hospital, NO, 22, Section 2, Ming Sheng Road, Tainan, 70343, Taiwan
BMC Cell Biol 4:14. 2003....
- Effects of follicular size of cytoplast donor on the efficiency of cloning in cattleJorge A Piedrahita
Department of Veterinary Anatomy and Public Health, Texas A and M University, College Station, Texas 77843 4458, USA
Mol Reprod Dev 61:317-26. 2002..No differences in survival to term (2/13 in each group) were observed. These results indicate that cytoplasts from follicles of 1-3 and 6-12 mm in diameter are equally developmentally competent when used in a nuclear transfer procedure...
- Effects of granulosa coculture on in-vitro oocyte meiotic maturation within a putatively less competent murine modelBoon Chin Heng
Department of Obstetrics and Gynaecology, Faculty of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074
Theriogenology 62:1066-92. 2004..ECM gel had a detrimental effect on fertilization and developmental competence, even though it had no detrimental effect on meiotic maturation itself...
- Sequential assessment of individually cultured human embryos as an indicator of subsequent good quality blastocyst developmentE Neuber
Yale Center for Reproductive Medicine and Infertility, Yale University School of Medicine, New Haven, CT, USA
Hum Reprod 18:1307-12. 2003..In this study, an investigation was made to determine if developmental markers on the day of fertilization (day 1) can predict good subsequent blastocyst development...
- Mouse embryos generated from frozen-thawed oocytes can successfully survive a second cryopreservationAriel Revel
In Vitro Fertilization Unit, Department of Obstetrics and Gynecology, Hadassah University Hospital, Ein Kerem, Jerusalem, Israel
Hum Reprod 19:666-9. 2004..To determine whether mouse embryos generated from frozen-thawed oocytes can successfully survive a second cryopreservation...
- Selection of prepubertal goat oocytes using the brilliant cresyl blue testE Rodriguez-Gonzalez
Departament de Ciencia Animal i dels Aliments, Facultat de Veterinaria, Universitat Autonoma de Barcelona, Spain
Theriogenology 57:1397-409. 2002..05) in the group of BCB+ (41.3 and 12.0%, respectively) than in BCB- oocytes (21.3 and 3.6%, respectively). In conclusion, the BCB test is a useful way to select more competent prepubertal goat oocytes for in vitro embryo production...
- Exposure to androgens during in vitro maturation does not affect the developmental potential of porcine oocytesJ R Herrick
Department of Animal Sciences, The Ohio State University, Columbus 43210, USA
Theriogenology 58:1131-9. 2002..20) between oocytes exposed to androgens and oocytes not exposed to androgens. These results indicate that exposure to androgens during IVM does not affect the ability of oocytes to cleave or develop up to the blastocyst stage in vitro...
- Preimplantation and postimplantation development of rat embryos cloned with cumulus cells and fibroblastsP Iannaccone
Department of Pediatrics, Northwestern University Medical School and Children's Memorial Institute for Education and Research, Chicago, Illinois 60614, USA
Zygote 9:135-43. 2001..These results suggest that the nuclei of cultured rat cells, even following genetic modification, can be reprogrammed to support early embryonic development, which is a prerequisite to cloning the rat...
- Vitrification of rat embryos at various developmental stagesM S Han
The Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
Theriogenology 59:1851-63. 2003..These results show that rat embryos at the 2-cell to blastocyst stages can be vitrified with EFS40, and that the morula stage is the most feasible stage for embryo cryopreservation in this species...
- Expression and role of the ether-à-go-go-related (MERG1A) potassium-channel protein during preimplantation mouse developmentN J Winston
Department of Physiology and Institute for Biomedical Research, School of Biomedical Sciences, University of Sydney, New South Wales 2006, Australia
Biol Reprod 70:1070-9. 2004..These results show that MERG1A is developmentally regulated and suggest that it might play a role in early mouse embryogenic development...
- In vitro development and mitochondrial fate of macaca-rabbit cloned embryosCai Xia Yang
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Mol Reprod Dev 65:396-401. 2003....
- Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expressionD Rizos
Department of Animal Science and Production, University College Dublin, Lyons Research Farm, Newcastle, County Dublin, Ireland
Biol Reprod 68:236-43. 2003..The challenge now is to try and bridge this gap...
- Evaluation of mouse preimplantation embryos exposed to oxidative stress cultured with insulin-like growth factor I and II, epidermal growth factor, insulin, transferrin and seleniumRafał Kurzawa
Clinic for Reproduction and Gynecology, Pomeranian Academy of Medicine, Szczecin, Poland
Reprod Biol 2:143-62. 2002..IGF-I, IGF-II, EGF and ITS have a positive effect on pre-implantation embryo development in detrimental culture conditions of oxidative stress...
- The effect of extracellular matrix molecules on mouse preimplantation embryo development in vitroF Figueiredo
Monash Institute of Reproduction and Development, Monash University, Monash Medical Centre, 246 Clayton Road, Clayton, Victoria 3168, Australia
Reprod Fertil Dev 14:443-51. 2002..Heparan sulfate proteoglycan increases inner cell mass numbers and this may be due to interactions with the growth factors fibroblast growth factor 4 (FGF-4) and granulocyte-macrophage colony-stimulating factor...
- Protection against reactive oxygen species during mouse preimplantation embryo development: role of EDTA, oxygen tension, catalase, superoxide dismutase and pyruvateN M Orsi
Department of Biology, University of York, York, United Kingdom
Mol Reprod Dev 59:44-53. 2001..The results suggest that embryo culture in EDTA-free medium under 5% O2 provides the most practical and physiological conditions for in vitro murine embryo culture...
- Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo HCG primingWeon Young Son
In Vitro Fertilization Laboratory, Maria Infertility Hospital, Dongdaemun Gu, Seoul, Korea
Hum Reprod 17:134-6. 2002..This approach opens a potential for a new dimension in the management of patients with PCOS...
- Silicone oil used in microdrop culture can affect bovine embryonic development and freezabilityA Van Soom
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
Reprod Domest Anim 36:169-76. 2001..It appears that due to the changed lipid contents of the medium, embryonic membranes are rendered more susceptible to freezing due to altered membrane composition or by membrane damage caused by lipid peroxidation...
- Causes of developmental failure of in-vitro matured rhesus monkey oocytes: impairments in embryonic genome activationR Dee Schramm
Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
Hum Reprod 18:826-33. 2003....
- Nuclear-cytoplasmic "tug of war" during cloning: effects of somatic cell nuclei on culture medium preferences of preimplantation cloned mouse embryosYoung Gie Chung
The Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 North Broad Street, Philadelphia, PA 19140, USA
Biol Reprod 66:1178-84. 2002..The relevance of this to understanding the kinetics and mechanisms of nuclear reprogramming and to the eventual improvement of the overall success in cloning is discussed...
- Overnight incubation improves selection of frozen-thawed blastocysts for transfer: preliminary study using supernumerary embryosFabrice Guerif
Physiopathologie de la Reproduction, Unité Mixte de Recherche 6073, Physiologie de la Reproduction et des Comportements, INRA CNRS Université de Tours, CHU Bretonneau, 37044 Tours Cedex, France
Theriogenology 60:1457-66. 2003..Increasing the interval between thawing and transfer appears to be beneficial in order to better select for transfer frozen-thawed blastocysts...
- Efects of the pine needle abortifacient, isocupressic acid, on bovine oocyte maturation and preimplantation embryo developmentS Wang
USDA ARS, US Sheep Experiment Station, Dubois, ID 83423, USA
Anim Reprod Sci 81:237-44. 2004..In conclusion, ICA showed no adverse effects on oocyte maturation and preimplantation embryo development in vitro or subsequent viability in vivo using the ICA concentrations and in vitro culture parameters of this study...
- A prospective trial of blastocyst culture and transferTakafumi Utsunomiya
St Luke Clinic, 5 Tsumori Tomioka, Oita City 870 0947, Japan
Hum Reprod 17:1846-51. 2002..The aim of this study was to evaluate the effectiveness of the blastocyst culture method compared with the conventional day 3 transfer method using a prospective trial...
- Ultrastructural morphometry of bovine blastocysts produced in vivo or in vitroA E Crosier
Departments of Animal Science, North Carolina State University, Raleigh 27695-7621, USA
Biol Reprod 64:1375-85. 2001..However, the specific nature of these deviations varied with the type of culture conditions used for in vitro embryo production...
- In vitro culture and interferon-tau secretion by ovine blastocystsW Caroline Lo
Research Institute for Animal Production, Bogor, Indonesia, Australia
Anim Reprod Sci 70:191-202. 2002..The mean concentration of IFN-tau secreted by day-8 embryos after 1 day in culture (10.99+/-2.55 ng/ml) was not significantly different to day-7 embryos after 2 days in culture (8.8+/-1.75 ng/ml)...
- Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrificationT L Nedambale
Connecticut Center for Regenerative Biology, University of Connecticut, 1392 Storrs Rd, U 4233, Storrs, CT 06269 4223, USA
Theriogenology 62:437-49. 2004..Vitrification using VS3a may be used more effectively to cryopreserve in vitro produced embryos than the conventional slow freezing method...
- Improved cleavage of bovine ICSI ova cultured in heparin-containing mediumGuang Peng Li
Animal Reproduction and Biotechnology Laboratory, Foothills Campus, Colorado State University, Fort Collins, CO 80523, USA
Theriogenology 61:1077-84. 2004..05)...
- Apolipoprotein E receptors are required for reelin-induced proteasomal degradation of the neuronal adaptor protein Disabled-1Hans H Bock
Department of Medicine II, Albert Ludwigs Universitat, Albertstrasse 23, 79104 Freiburg, Germany
J Biol Chem 279:33471-9. 2004....
- Blastocyst culture in human IVF: the final destination or a stop along the way?A L Smith
Fertility Lab Consulting, San Antonio, Texas 78230, USA
Theriogenology 57:97-107. 2002..Culture of human embryos to the blastocyst stage can be an effective method for improving implantation rates and reducing the high order multiple pregnancy rates seen in human IVF clinics when more than three embryos are transferred...
- [Study on in vitro culture and cryopreservation by vitrification of blastocysts derived from single mouse 2-cell embryos' blastomeres]Q G Meng
College of Animal Science, China Agricultural University, Beijing 100094
Shi Yan Sheng Wu Xue Bao 34:329-31. 2001..However, the survival rates increased to 64% and 70% when the blastocysts were vitrified(one-step method) ater equilibration in EFS40 at 25 degrees C for 0.5-1 min...
- Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocystWoo Suk Hwang
College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
Science 303:1669-74. 2004..Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells...
- Bile acids in the assessment of hepatocellular functionM B Thompson
Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
Toxicol Pathol 24:62-71. 1996..Recent developments in culture techniques permit long-term maintenance of functionally stable, differentiated cells...
- Soil-transmitted helminthiases: nationwide survey in ChinaL Q Xu
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai
Bull World Health Organ 73:507-13. 1995..By stool examinations using the Kato-Katz thick-smear and larval-culture techniques, overall prevalences of 47.0%, 18.8%, and 17...
- [Is there a reliable new diagnostic procedure in mycobacterial infections including tuberculosis?]R Urbanczik
Med Diagnost Institut, Schiffweiler
Pneumologie 48:156-9. 1994..monitor drug resistance rates (pulmonary TB) and with other forms of TB or other mycobacterioses radiometric culture techniques, particularly if combined with conventional egg media cultures and with identification of grown bacilli ..
- New prospects for the diagnosis of viral infectionsR H Yolken
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland
Yale J Biol Med 62:131-9. 1989..diagnostic assays is limited by the time, expense, and expertise required for the performance of tissue culture techniques. Similarly, the application of immunoassay techniques has been inhibited by the limited degrees of ..
- Sexually transmitted virusesF Rapp
Department of Microbiology and Immunology, Pennsylvania State University, College of Medicine, Hershey
Yale J Biol Med 62:173-85. 1989..human pathogens has been learned only recently, owing to the advent of DNA technologies and advances in culture techniques. In fact, our awareness of one virally transmitted venereal disease, acquired immunodeficiency syndrome, ..
- Maintenance of cell viability in stored boneN J Treble
Royal Liverpool Hospital, University of Liverpool, England
Spine (Phila Pa 1976) 15:830-2. 1990..By the use of tissue culture techniques, the authors show that it is possible to keep bone cells alive for many weeks...
- [Comparison of amplified Mycobacterium Tuberculosis Direct Test (MTD), Amplicor Mycobacteria kit (Amplicor) and PCR method for detection Mycobacterium tuberculosis in clinical specimens]M Goto
Department of Infectious Diseases, University of Tokyo
Kansenshogaku Zasshi 69:539-45. 1995..These were shown to be at least as sensitive as the conventional culture techniques. The Tokyo Univ. method using radioisotope, takes up to 3 days, on the other hand 2 kits take 4-5 hours...
- The diagnosis of human immunodeficiency virus infection: progress in less than five yearsF J Michalski
Diagnostic Virology Laboratory, MetPath, Inc, Teterboro, NJ 07608
Yale J Biol Med 62:93-102. 1989..period of time since then, we have witnessed improvements in antibody detection methods, refinements in culture techniques, and the introduction of antigen and nucleic acid detection methods, including the polymerase chain reaction...
- A transversally isotropic elasto-damage constitutive model for the periodontal ligamentA N Natali
Centre of Mechanics of Bilogical Materials, University of Padova, Via Marzolo 9, I 35131, Italy
Comput Methods Biomech Biomed Engin 6:329-36. 2003..A finite element analysis is presented in order to define a general numerical approach. A comparison of numerical and experimental data is provided in order to show the reliability and effectiveness of the formulation assumed...
- Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak treesI Hernandez
Instituto Madrileño de Investigación Agraria y Alimentaria, Apdo 127, 28800 AlcalA de Henares, Madrid, Spain
Plant Cell Rep 21:765-70. 2003..Although the mean percentage of recovery for the whole process was low, plants could be regenerated from four of the five trees tested...
- Transfer of inflammatory cytokines across the placentaMichael V Zaretsky
Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9032, USA
Obstet Gynecol 103:546-50. 2004..The purpose of this study was to determine whether the placental transfer of interleukin (IL)-1alpha, IL-6, and tumor necrosis factor-alpha (TNF-alpha) occurs...
- Pro- or anti-oxidant manganese: a suggested mechanism for reconciliationDiem HaMai
Department of Community and Environmental Medicine, Center for Occupational and Environmental Health, University of California, Irvine, CA 92692 1820, USA
Neurochem Int 44:223-9. 2004....
- Novel Determination Of Chondrocyte Material PropertiesCLARK HUNG; Fiscal Year: 2004..Successful implementation of the aforementioned will permit new research questions regarding the role of biomechanical influences in the development and progression of OA to be addressed. ..
- Chondrocyte Mechanotransduction Using MicrofluidicsCLARK HUNG; Fiscal Year: 2009....
- Intervertebral Disc Response to Cyclic Loading in VivoCLARK HUNG; Fiscal Year: 2005..abstract_text> ..
- Quantitating Absorption of Complex Chemical MixturesJim Riviere; Fiscal Year: 2009..abstract_text> ..
- ANTI-VIRAL TREATMENT OF DEVELOPING BOVINE EMBRYOSMaurice Givens; Fiscal Year: 2002....
- Intracellular signaling in anesthetic and hypoxic preconditioning of neuronsPhilip E Bickler; Fiscal Year: 2010..The work will also identify why the aging brain may not be able to benefit from the neuroprotective actions of anesthetics or other preconditioning treatments. ..
- Quantitative Microfluidic Molecular EvolutionBRIAN PAEGEL; Fiscal Year: 2006....
- Role of the CD40/CD40L dyad in atherosclerosisMasanori Aikawa; Fiscal Year: 2006..abstract_text> ..
- Regulation of lipolysis by nitric oxide and adenosineRobert Hickner; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Assessment of Tissue Response to StressJoan Sanders; Fiscal Year: 2006..this technology could be applied to other areas of rehabilitation, for example, shoe design for patients with insensate feet, cushion design for wheelchair users, and mattress design for bedridden patients [unreadable] [unreadable]..
- Metabolism and Excitability in Rodent HippocampusAnne Williamson; Fiscal Year: 2006..abstract_text> ..
- Mechanism of cytochrome P450 uncoupling and inactivationJARED GOLDSTONE; Fiscal Year: 2005..Recently identified AhR-agonistic persistent pollutants, such as polybrominated diphenyl ethers will also be investigated. ..
- GDNF Protection in the Inner EarYehoash Raphael; Fiscal Year: 2005..The data we propose to generate will enhance our understanding of the mechanisms of action of GDNF in normal and injured inner ear epithelia, knowledge that may eventually lead to better clinical treatments ..
- Skin Adaptation to Mechanical StressJoan Sanders; Fiscal Year: 2006..By understanding the process of skin adaptation (natural skin strengthening) at both a cellular and molecular level, therapies to encourage skin adaptation before breakdown occurs can be intelligently pursued. ..
- Novel Design for Artificial Transcription FactorsDusan Stanojevic; Fiscal Year: 2006..The results of this Phase I research may have important implications in pharmaceutical drug development and may provide new tools for basic research in molecular genetics. [unreadable] [unreadable] [unreadable]..
- Age-Dependent Response of Hippocampal Neurons to StressGregory Brewer; Fiscal Year: 2006..abstract_text> ..
- C. Pneumoniae and atherosclerotic plaque destabilizationMichael E Rosenfeld; Fiscal Year: 2010..4. To determine whether exposure of young versus old mice to air pollution increases the susceptibility of the mice to C. pneumoniae infection and accelerates the development and progression of atherosclerosis. ..
- IMMUNOPATHOGENESIS OF PSORIASISBrian Nickoloff; Fiscal Year: 2009....
- 14th Biennial FASEB Summer Research Conference on RetinoidsSusan Smith; Fiscal Year: 2008..Its goal is to stimulate scientific exchange and new investigations that will provide needed insights into retinoid roles in disease prevention and treatment. [unreadable] [unreadable] [unreadable]..
- Neural Crest Genes in Craniofacial DevelopmentLAURA GAMMILL; Fiscal Year: 2008..The long-term career goal is to run a laboratory doing basic research in neural crest and craniofacial development. [unreadable] [unreadable]..
- ECI Conference on Engineering Cell Biology II - The Cell in ContextLinda Griffith; Fiscal Year: 2007..This conference will also emphasize translation of basic research into industry application in tissue engineering and drug development. [unreadable] [unreadable] [unreadable]..
- Calcium Signaling & Prefrontal Deficits in SchizophreniaDavid McCormick; Fiscal Year: 2008..abstract_text> ..
- INTERFACE MECHANICS, MATERIALS AND TISSUE RESPONSEJoan Sanders; Fiscal Year: 2007..The development of methods to predict and overcome the detrimental effects of residual limb shape change and ultimately prevent soft-tissue breakdown will help to prevent secondary disability and morbidity in the amputee population. ..
- Susceptibility mechanism in alcoholic pancreatitisHerbert Gaisano; Fiscal Year: 2007..Significance: These actions of alcohol on pancreatic acinar PM-Munc18c may be the 'sentinel' event that initiates the role of alcohol in pancreatic injury, and which could be therapeutically targeted. ..
- Novel Molecular Tools for Transsynaptic Circuit AnalysisHaig Keshishian; Fiscal Year: 2007..unreadable] [unreadable]..
- CELLULAR MECHANISMS OF VISUAL CORTICAL FUNCTIONDAVID A contact MCCORMICK; Fiscal Year: 2010..Our studies will give fundamental information relevant to understanding not only vision, but also attention and memory. ..
- Proximal Tubule Transport Defect in Hyp MiceMichel Baum; Fiscal Year: 2007..These studies will not only examine the pathogenesis of this disorder, but will directly examine if indomethacin potentially represents a novel therapy for this disease. ..
- PEDIATRIC NEPHROLOGY TRAININGMichel Baum; Fiscal Year: 2005..Fellows also attend seminars in basic science departments. The research mentor, as well as the program director, closely supervise trainees to ensure that the goals of the training program are achieved. ..
- CRANIOFACIAL MORPHOGENESIS IN PRENATAL ALCOHOL EXPOSURESusan Smith; Fiscal Year: 2005..Results also may provide possible explanations for genetic-based variations in fetal responses to prenatal ethanol exposure. ..
- Dietary Cysteine:Conceptal Response to Oxidative StressCraig Harris; Fiscal Year: 2002....
- ESTROGEN STATUS AND THE FUNCTION OF CORONARY ARTERIESVIRGINIA MILLER; Fiscal Year: 2002..The experiments of this proposal take such an approach using molecular to integrated whole animal physiology. ..
- STRUCTURE/FUNCTION OF SUBCORTICAL WHITE MATTER NEURONSAnne Williamson; Fiscal Year: 2002..Given the possible involvement of these cells in a variety of neurological disorders, it is critical to characterize these cells completely. ..
- Abnormalities in Keratoconus CorneasMARIA KENNEY; Fiscal Year: 2003..These studies will provide fundamental insights into KC pathogensis and may provide a basis for therapy. ..
- Exercise, muscle nutritive blood flow and eNOS in agingRobert Hickner; Fiscal Year: 2003..abstract_text> ..
- Estrogen: Chrodroprotective or Destructive?Sally Frenkel; Fiscal Year: 2002..The inconclusive and contradictory data from human and animal studies render the proposed studies highly significant. ..
- A POXVIRAL PROTEIN THAT INHIBITS CHEMOKINE FUNCTIONMitchell Krathwohl; Fiscal Year: 2003..4) To determine the regions of MC148R that are important for blocking chemotaxis by constructing mutant proteins at both the amino terminus and the internal heparin binding site. ..
- Volume Regulatory Pathways in Cystic Fibrosis MiceWon Kyoo Cho; Fiscal Year: 2003..abstract_text> ..
- MECHANISMS OF PROSTAGLANDIN INDUCED INTESTINAL REPAIRAnthony Blikslager; Fiscal Year: 2002..These experiments will provide insight into the signaling pathways and mechanisms by which prostaglandins trigger repair of acutely-injured intestinal epithelium. ..
- METHOD FOR DISCOVERING NOVEL TRANSCRIPTIONAL REGULATORSDusan Stanojevic; Fiscal Year: 2004..2) Developing a robust and rapid assay for testing of ATFs in human cell culture lines. 3) Demonstration of the ability of ATFs to target selected endogenous human genes in tissue culture. ..
- Gene Expression Analysis in Microcaptured Retinal CellsRuben Adler; Fiscal Year: 2005..The potential impact of the identification of these molecules is clear, since they could offer new avenues for the treatment of these devastating diseases. ..
- MHC-Defined and Identical Twin Monkeys for ResearchJoseph Kemnitz; Fiscal Year: 2005..Additionally, genetically identical macaques will have general utility in biomedical research, especially to the neuroscience community. ..
- PHENOTYPE AND FUNCTION OF SENESCENT KERATINOCYTESBrian Nickoloff; Fiscal Year: 2005....
- MECHANISMS OF PRETERM LABOR: NF-KB ACTIVATION OF COX-2DOUGLAS KNISS; Fiscal Year: 2001....
- INTERFACE MECHANICS, MATERIALS, AND TISSUE RESPONSEJoan Sanders; Fiscal Year: 2002....
- Role of BDNF in development and maintenance of taste bu*LINDA BARLOW; Fiscal Year: 2002..By using conditional, tissue specific knockouts, we will be able to separate the effects of elimination of BDNF in adulthood from its trophic effects already documented for the embryonic period. ..
- The Role of NO in the Regulation of Heart ContractilityBEATA WOLSKA; Fiscal Year: 2004..Our experiments provide new insights into the mechanisms and importance of NO (NOx) in alteration of cardiac contractility in physiological and pathological conditions. ..
- HPV DIFFERENTIATION DEPENDENT REPLICATIONCraig Meyers; Fiscal Year: 2003..abstract_text> ..
- Genetic Analysis of Papillomavirus Virion MorphogenesisCraig Meyers; Fiscal Year: 2009....
- Is S-adenosyl Methionine a Nutrition: Genetic Link in AD?Thomas Shea; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Diesel Exhaust and Atherosclerotic Plaque StabilityMichael Rosenfeld; Fiscal Year: 2007....
- Chemoprevention of Uterine Cancer Risk by AntioxidantsMinoti Sharma; Fiscal Year: 2003..The long-term goal of this proposal is to evaluate if the combination of Tam or estrogen with nontoxic antioxidants will cooperate to reduce uterine cancer risk associated with chronic exposure to Tam or estrogen. ..
- Solamere QLC100 Nipkow Spinning Disk Confocal UnitJONATHAN JAGGAR; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Cell Death in Normal and Diseased Human EpidermisBrian Nickoloff; Fiscal Year: 2006..This grant features a transition of experimental protocols beginning with conventional tissue culture techniques, and then moving to the use of living epidermal equivalents that contain stratified layers of KCs that can ..
- 3D Adipose Tissue Generation and Functional VerificationCharles Patrick; Fiscal Year: 2004..abstract_text> ..
- H. Pylory factors associated with peptic ulcer diseaseTIMOTHY COVER; Fiscal Year: 2006..pylori factors that help to determine clinical outcome, and ultimately, may lead to advances in the treatment or prevention of H. pylori-associated human diseases. [unreadable] [unreadable]..
- DEFORMATION-DEPENDENT ENZYME CLEAVAGE OF COLLAGENPeter Torzilli; Fiscal Year: 2005..The findings from this study could fundamentally change our concepts of matrix enzyme function and the design of intervention approaches to include the effect the mechanical environment has on the ECM. ..
- RNAi Mediated Gene Disruption in Caenorhabditis ElegansWayne Van Voorhies; Fiscal Year: 2006..Such worms could identify candidate genes affecting processes that act "downstream" from the metabolic rate and provide valuable insights into the causes of aging and mechanisms by which it can be attenuated. ..
- NEW BIOMATERIALS TO MINIMIZE SCAR TISSUE FORMATIONJoan Sanders; Fiscal Year: 2002..e. soft-tissue complications that require surgical repair through use of a biomaterial. The new materials will prevent scar tissue formation and reduce the occurrence of secondary complications. ..
- TENDON FIBER-MATRIX MECHANICS--THEORY AND EXPERIMENTSDawn Elliott; Fiscal Year: 2003..This information will enable us to develop and assess potential treatment modalities. ..
- Regulation of Pancreas Development by P13K/Akt SignalingErnesto Bernal Mizrachi; Fiscal Year: 2006..abstract_text> ..
- Role of SOCS in Regulation of Transplantation ToleranceStanislaw Stepkowski; Fiscal Year: 2004..Such an approach will directly evaluate whether Stat5 or Stat6 activation is regulated by SOCS3 and/or other SOCS. These experiments will provide the first evidence for the role of SOCS in induction of transplantation tolerance. ..
- Regulation of IL-4/IL-4R Signaling Pathway Mediate Tole*Stanislaw Stepkowski; Fiscal Year: 2003..This study may provide evidence that tolerogenic vaccines induce potent Jak/Stat regulatory proteins, which are instrumental in tolerance induction. ..
- Characterizing Tissue Development Under Biaxial LoadingAlvin Yeh; Fiscal Year: 2006..This novel experimental platform will form the basis for novel and innovative studies in mechanobiology. ..
- QUANTITATIVE MICROBIOLOGIC MODEL FOR PRETERM DELIVERYAndrew Onderdonk; Fiscal Year: 2004....