culture techniques

Summary

Summary: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or EMBRYO in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.

Top Publications

  1. ncbi Effects of beta-mercaptoethanol on formation of pronuclei and developmental competence of swamp buffalo oocytes
    N Songsasen
    Department of Biological Science, University of New Orleans, New Orleans, LA 70131, USA
    Anim Reprod Sci 71:193-202. 2002
  2. ncbi Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    J Ind Microbiol Biotechnol 40:41-9. 2013
  3. ncbi Notch signaling regulates the pattern of auditory hair cell differentiation in mammals
    A Zine
    Institute of Physiology, University of Lausanne, Switzerland
    Development 127:3373-83. 2000
  4. pmc Association between dental pulp stones and calcifying nanoparticles
    Jinfeng Zeng
    Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology and Institute of Stomatological Research, Sun Yat Sen University, Guangzhou, China
    Int J Nanomedicine 6:109-18. 2011
  5. ncbi Transplantation of cultured bone cells using combinations of scaffolds and culture techniques
    Toshimasa Uemura
    Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, Tsukuba, Ibaraki 305 8562, Japan
    Biomaterials 24:2277-86. 2003
  6. pmc A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat culture
    Holger Janssen
    Institut für Biowissenschaften, Universitat Rostock, Germany
    Appl Microbiol Biotechnol 87:2209-26. 2010
  7. ncbi Evaluation of a galactose-carrying gelatin sponge for hepatocytes culture and transplantation
    Sung Ran Hong
    School of Chemical Engineering, College of Engineering, Hanyang University, Seungdong ku, Seoul, 133 791, Korea
    J Biomed Mater Res A 67:733-41. 2003
  8. ncbi Cinnamic-derived acids significantly affect Fusarium graminearum growth and in vitro synthesis of type B trichothecenes
    Nadia Ponts
    Department of Cell Biology and Neuroscience, University of California Riverside, CA, USA
    Phytopathology 101:929-34. 2011
  9. pmc Changes in var gene mRNA levels during erythrocytic development in two phenotypically distinct Plasmodium falciparum parasites
    Madeleine Dahlbäck
    Centre for Medical Parasitology at Department of International Health, Immunology and Microbiology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark
    Malar J 6:78. 2007
  10. ncbi Engineering micropatterned surfaces for the coculture of hepatocytes and Kupffer cells
    Yekaterina S Zinchenko
    Mechanical Engineering Department, University of North Carolina at Charlotte, 9201 University City Blvd, Charlotte, NC 28223, USA
    J Biomed Mater Res A 75:242-8. 2005

Detail Information

Publications241 found, 100 shown here

  1. ncbi Effects of beta-mercaptoethanol on formation of pronuclei and developmental competence of swamp buffalo oocytes
    N Songsasen
    Department of Biological Science, University of New Orleans, New Orleans, LA 70131, USA
    Anim Reprod Sci 71:193-202. 2002
    ..This study provides information for further investigations on optimizing a system for in vitro production of swamp buffalo embryos...
  2. ncbi Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii
    Joel Farkas
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    J Ind Microbiol Biotechnol 40:41-9. 2013
    ..We report new growth media and culture techniques for Caldicellulosiruptor bescii, the most thermophilic cellulolytic bacterium known...
  3. ncbi Notch signaling regulates the pattern of auditory hair cell differentiation in mammals
    A Zine
    Institute of Physiology, University of Lausanne, Switzerland
    Development 127:3373-83. 2000
    ..Our data suggest that the Notch1 signaling pathway is involved in a complex interplay between the consequences of different ligand-Notch1 combinations during cochlear morphogenesis and the phases of hair cell differentiation...
  4. pmc Association between dental pulp stones and calcifying nanoparticles
    Jinfeng Zeng
    Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology and Institute of Stomatological Research, Sun Yat Sen University, Guangzhou, China
    Int J Nanomedicine 6:109-18. 2011
    ..CNPs could be detected and isolated from a high percentage of dental pulp stones, suggesting that CNPs might play an important role in the calcification of dental pulp...
  5. ncbi Transplantation of cultured bone cells using combinations of scaffolds and culture techniques
    Toshimasa Uemura
    Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 4, Tsukuba, Ibaraki 305 8562, Japan
    Biomaterials 24:2277-86. 2003
    ..this therapy, there remain several problems to be overcome, for example, the improvements of scaffolds and culture techniques. In this review article, two kinds of porous ceramics, a novel sintered porous hydroxyapatite and a porous ..
  6. pmc A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat culture
    Holger Janssen
    Institut für Biowissenschaften, Universitat Rostock, Germany
    Appl Microbiol Biotechnol 87:2209-26. 2010
    ..Thus, a first systematic study of acidogenic and solventogenic chemostat cultures is presented, and similarities as well as differences to previous studies of batch cultures are discussed...
  7. ncbi Evaluation of a galactose-carrying gelatin sponge for hepatocytes culture and transplantation
    Sung Ran Hong
    School of Chemical Engineering, College of Engineering, Hanyang University, Seungdong ku, Seoul, 133 791, Korea
    J Biomed Mater Res A 67:733-41. 2003
    ..Hepatic specific metabolic functions, namely, the secretion of serum albumin and the synthesis of urea, were well maintained and promoted by spheroidal hepatocytes formed in the MGLA sponge...
  8. ncbi Cinnamic-derived acids significantly affect Fusarium graminearum growth and in vitro synthesis of type B trichothecenes
    Nadia Ponts
    Department of Cell Biology and Neuroscience, University of California Riverside, CA, USA
    Phytopathology 101:929-34. 2011
    ..These data suggest that, in planta, different compositions in phenolic acids of kernels from various cultivars may reflect different degrees of sensitivity to "mycotoxinogenesis."..
  9. pmc Changes in var gene mRNA levels during erythrocytic development in two phenotypically distinct Plasmodium falciparum parasites
    Madeleine Dahlbäck
    Centre for Medical Parasitology at Department of International Health, Immunology and Microbiology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark
    Malar J 6:78. 2007
    ..Two parasite populations were studied; an unselected population of NF54 and a selected population, NF54VAR2CSA, to compare both the transcription of var2csa and the expression pattern of the corresponding protein...
  10. ncbi Engineering micropatterned surfaces for the coculture of hepatocytes and Kupffer cells
    Yekaterina S Zinchenko
    Mechanical Engineering Department, University of North Carolina at Charlotte, 9201 University City Blvd, Charlotte, NC 28223, USA
    J Biomed Mater Res A 75:242-8. 2005
    ..To demonstrate the success of the method, surface characterization of the resultant micropatterns, as well as morphological and functional results are also presented...
  11. ncbi Detection and isolation of nanobacteria-like particles from urinary stones: long-withheld data
    Hiromi Kumon
    Department of Urology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Int J Urol 18:458-65. 2011
    ..To report our experimental results on detection and isolation of nanobacteria-like particles (NLP) from urinary stone samples...
  12. ncbi Effect of in vitro and in vivo culture on embryo development from prepubertal goat IVM-IVF oocytes
    D Izquierdo
    Department de Ciència Animal i dels Aliments, Universitat Autonoma de Barcelona, Spain
    Theriogenology 57:1431-41. 2002
    ....
  13. ncbi Molecular evidence of false-positive cultures for Mycobacterium tuberculosis in a Taiwanese hospital with a high incidence of TB
    Chih Cheng Lai
    Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, No 7 Chun Shan South Rd, Taipei 100, Taiwan
    Chest 137:1065-70. 2010
    ..Isolation of Mycobacterium tuberculosis (MTB) from the clinical specimens of patients with suspected TB remains the gold standard for diagnosis of TB. However, false-positive MTB cultures can occur as a result of laboratory contamination...
  14. ncbi Attempts at in vitro fertilization and culture of in vitro matured oocytes in sei ( Balaenoptera borealis) and Bryde's ( B. edeni) whales
    M M U Bhuiyan
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080 8555 Hokkaido, Japan
    Zygote 17:19-28. 2009
    ..1%) in sei whales. In conclusion, the present study indicates that IVF in sei whales is possible to achieve cleaved embryos developing to morula stage. This is the first in vitro embryo production attempt in sei and Bryde's whales...
  15. ncbi Identification and characterisation of mRif1: a mouse telomere-associated protein highly expressed in germ cells and embryo-derived pluripotent stem cells
    Ian R Adams
    Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, Cambridge, United Kingdom
    Dev Dyn 229:733-44. 2004
    ..mRif1 may be involved in the maintenance of telomere length or pluripotency in the germline and during early mouse development...
  16. ncbi Comparison of nucleic acid amplification tests and culture techniques in the detection of Neisseria gonorrhoeae and Chlamydia trachomatis in victims of suspected child sexual abuse
    Nancy D Kellogg
    Department of Pediatrics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
    J Pediatr Adolesc Gynecol 17:331-9. 2004
    ..2) To compare Ligase Chain Reaction (LCR), Polymerase Chain Reaction (PCR), and culture methods in the detection of chlamydia and gonorrhea infection among prepubertal and adolescent girls referred for sexual abuse evaluations...
  17. ncbi Effect of donor stimulation, frozen semen and heparin treatment on the efficiency of in vitro embryo production in goats
    Lucyna Katska-Ksiazkiewicz
    Department of Animal Reproduction, National Research Institute of Animal Production, 32 083 Balice Krakow, Poland
    Theriogenology 62:576-86. 2004
    ..Overall, more than 60% of the recipients became pregnant and 20% of all transferred embryos survived delivering 13 healthy kids. Our caprine IVP system allows obtaining embryos with developmental competence comparable to bovine IVP...
  18. ncbi Cultivation of explants of the marine sponge Crambe crambe in closed systems
    El Hassan Belarbi
    Chemical Engineering Department, University of Almeria, 04071 Almeria, Spain
    Biomol Eng 20:333-7. 2003
    ..The sponges grew better in CFTHS compared with the DFTHS. The high growth rates observed in CFTHS suggest that this technique is a promising method for culturing C. crambe in closed systems...
  19. ncbi Biotechnological aspects of marine sponges
    Ronald Osinga
    J Biotechnol 100:91-2. 2003
  20. ncbi Apoptosis in the preimplantation mouse embryo: effect of strain difference and in vitro culture
    Marziel Kamjoo
    School of Biological Sciences, University of Manchester, Stopford Building, Oxford Rd, Manchester, United Kingdom
    Mol Reprod Dev 61:67-77. 2002
    ..0005 M16; P = 0.002 kSOM) and than C57BL6/CBA x C57BL6/CBA in M16 (P = 0.019). Our data suggest that genetic make-up and the chemical composition of simple medium are equally important in determining the level of apoptosis...
  21. ncbi Heart-valve mesenchyme formation is dependent on hyaluronan-augmented activation of ErbB2-ErbB3 receptors
    Todd D Camenisch
    College of Pharmacy and Steele Memorial Children s Research Center, University of Arizona, Tucson, Arizona, USA
    Nat Med 8:850-5. 2002
    ..These data provide a link between extracellular matrix-hyaluronan and ErbB receptor activation during development of early heart-valve and septal mesenchyme...
  22. ncbi Embryonic mouse submandibular salivary gland morphogenesis and the TNF/TNF-R1 signal transduction pathway
    M Melnick
    Laboratory for Developmental Genetics, University of Southern California, Los Angeles, California 90089 0641, USA
    Anat Rec 262:318-30. 2001
    ..The results of these experiments indicate that the TNF/TNF-R1 signal transduction pathway plays an important role in balancing cell proliferation and apoptosis during SMG duct and presumptive acini formation...
  23. ncbi Automated synchronization of Plasmodium falciparum parasites by culture in a temperature-cycling incubator
    J David Haynes
    Department of Immunology, Walter Reed Army Institute of Research, Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
    Methods Mol Med 72:489-97. 2002
  24. ncbi CUC2 as an early marker for regeneration competence in Arabidopsis root explants
    Hans Motte
    Department of Plant Production, University College Ghent, member of the Ghent University Association, Schoonmeersstraat, 52, BE 9000 Ghent, Belgium
    J Plant Physiol 168:1598-601. 2011
    ..Thus, CUC2 is a predictive marker for the acquisition of root explant competence for root and shoot organogenesis...
  25. pmc Hyperglycemia-induced vasculopathy in the murine vitelline vasculature: correlation with PECAM-1/CD31 tyrosine phosphorylation state
    E Pinter
    Departments of Pathology and Pediatrics, Yale University, Alexion Pharmaceuticals, Inc, New Haven, Connecticut, USA
    Am J Pathol 154:1367-79. 1999
    ....
  26. ncbi Association between self-replicating calcifying nanoparticles and aortic stenosis: a possible link to valve calcification
    Miguel A Bratos-Pérez
    Servicio de Microbiologia, Facultad de Medicina, Hospital Clinico Universitario, Valladolid, Spain
    Eur Heart J 29:371-6. 2008
    ..These CNPs have been detected and isolated from calcified human tissues, including blood vessels and kidney stones, and are referred to as nanobacteria. We evaluated calcific aortic valves for the presence of CNP...
  27. ncbi Developmental events and shoot apical meristem gene expression patterns during shoot development in Arabidopsis thaliana
    Andrew J Cary
    Boyce Thompson Institute and Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA
    Plant J 32:867-77. 2002
    ..In contrast, the expression pattern of the enhancer trap lines during zygotic embryogenesis was more localized to the presumptive SAM even in early stages of embryogenesis...
  28. ncbi Direct DNA delivery into zebrafish embryos employing tissue culture techniques
    R Sussman
    Marine Biological Laboratory, Marine Resources Center, Woods Hole, Massachusetts 02543, USA
    Genesis 31:1-5. 2001
    ..These results demonstrate the ease of obtaining transfected fish embryos, which facilitate the mass production of new genotypes and extend the procedure to laboratories with limited resources...
  29. ncbi Theileria annulata: in vitro cultivation of schizont-infected bovine lymphocytes
    V Shkap
    Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Vet Parasitol 65:11-20. 1996
    ..In Roller bottles, with the same type and volume of medium and cultivation for 7 days without medium change, best yield resulted from seeding with the highest inoculum size of 4 x 10(7) cells per vessel...
  30. ncbi Nuclear translocation of nuclear factor kappa B in early 1-cell mouse embryos
    A Nishikimi
    Laboratory of Reproductive Physiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Biol Reprod 60:1536-41. 1999
    ..Taken together, the results suggest that the activation of NF-kappaB at the early 1-cell stage is required for the development of mouse embryos beyond the 2-cell stage...
  31. ncbi Insulin and insulin-like growth factor-I promote rabbit blastocyst development and prevent apoptosis
    A Herrler
    Department of Anatomy and Reproductive Biology, RWTH University of Aachen, 52057 Aachen, Germany
    Biol Reprod 59:1302-10. 1998
    ..By immunohistochemistry we could show that addition of insulin (0.68-68 nM) decreased apoptosis and increased cell proliferation in a dose-dependent manner, supporting blastocyst development significantly...
  32. ncbi Effects of protein source and energy substrates on the in vitro development of bovine embryos in a two-step culture system
    Kwang Taek Lim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    J Vet Sci 4:73-8. 2003
    ..Therefore, the avoidance of this negative effect should allow more optimal conditions to be developed for in vitro production...
  33. ncbi Effects of donor oocytes and culture conditions on development of cloned mice embryos
    Shaorong Gao
    Department of Gene Expression and Development, The Roslin Institute, Roslin, Scotland, United Kingdom
    Mol Reprod Dev 66:126-33. 2003
    ..M16 medium is superior for culture of nuclear transfer embryos and low oxygen concentration with CZB medium during culture shows no benefit on development of cloned embryos...
  34. ncbi Double vitrification of rat embryos at different developmental stages using an identical protocol
    Vladimir Isachenko
    Department of Animal Production, Center for Agricultural Researches, P O Box 727, Zaragoza 50080, Spain
    Theriogenology 60:445-52. 2003
    ..The low survival rate of blastocysts subjected to double vitrification requires further investigation...
  35. ncbi Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitro
    S E Park
    College of Medicine, Pochon CHA University and Infertility Medical Center of CHA General Hospital, Seoul, South Korea
    Fertil Steril 76:167-74. 2001
    ..CONCLUSION(S): A modified P-1 medium was developed, and it supported the development of both in vivo- and in vitro-derived ICR mouse embryos...
  36. ncbi Effect of leukemia inhibitory factor on bovine embryos produced in vitro under chemically defined conditions
    S Sirisathien
    Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602 7389, USA
    Theriogenology 59:1751-63. 2003
    ..Data indicate that addition of LIF has no major beneficial effect on bovine embryos produced in these chemically defined conditions...
  37. ncbi Effects of growth hormone and insulin-like growth factor-I on development of in vitro derived bovine embryos
    F Moreira
    Department of Aninial Sciences, University of Florida, Gainesville 32611, USA
    Theriogenology 57:895-907. 2002
    ..In conclusion, both GH and IGF-I stimulate embryonic development in cattle and GH effects may likely involve IGF-I-independent mechanisms...
  38. ncbi Prevalence of apoptosis and inner cell allocation in bovine embryos cultured under different oxygen tensions with or without cysteine addition
    A Van Soom
    Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
    Theriogenology 57:1453-65. 2002
    ..Embryos cultured under 5% O2 in the presence of cysteine, however, possessed significantly higher numbers of ICM cells. This finding corroborates the theoretical assumption that antioxidants are beneficial for ICM development...
  39. ncbi Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study
    A van Langendonckt
    Department of Gynecology, Catholic University of Louvain, Brussels, Belgium
    Fertil Steril 76:1023-31. 2001
    ..1% and 36.1%, respectively). CONCLUSION(S): In our day 3 embryo transfer program, G1.2/G2.2 media were superior to Sydney IVF media, whereas both media yielded similar outcomes in our blastocyst transfer program...
  40. ncbi Effects of fetuin on zona pellucida hardening, fertilization and embryo development in cattle
    F C Landim-Alvarenga
    Department of Animal Reproduction and Veterinary Radiology, F M V Z UNESP, Botucatu, SP, Brazil
    Anim Reprod Sci 71:181-91. 2002
    ..Apparently fetuin can be used as a substitute for FCS during bovine oocyte maturation, since it leads to similar developmental rates as FCS in intact and partially stripped oocytes...
  41. ncbi Beta-alanine but not taurine can function as an organic osmolyte in preimplantation mouse embryos cultured from fertilized eggs
    Mary Anne Hammer
    Ottawa Health Research Institute Hormones, Growth and Development Program, Ontario
    Mol Reprod Dev 66:153-61. 2003
    ..Thus, the beta-amino acid transporter is likely present in early preimplantation embryos to supply beta-amino acids such as taurine for purposes other than to serve as organic osmolytes...
  42. ncbi In vitro production of embryos in swine
    L R Abeydeera
    PIC International, Berkeley, CA 94710, USA
    Theriogenology 57:256-73. 2002
    ..Further research efforts should be directed to improve oocyte/embryo quality and the methods to minimize polyspermy through development of novel IVM, IVF, and EC techniques...
  43. ncbi Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and quality
    Paloma Duque
    Area de Genética y Reproducción, SERIDA, Centro de Fertilización in vitro de Asturias, C Alvarez Garaya 12, 33206, Gijon, Spain
    Reprod Nutr Dev 43:487-96. 2003
    ..Both Milli-Q and Sigma supported embryo development at comparable rates, although in the presence of BSA, blastocysts obtained in the medium prepared with Milli-Q water had superior quality in terms of ICM/total cells rates...
  44. ncbi Influences of epidermal growth factor and insulin-like growth factor-I on bovine blastocyst development in vitro
    S Sirisathien
    Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602 7389, USA
    Anim Reprod Sci 77:21-32. 2003
    ..In conclusion, both EGF and IGF-I could independently enhance bovine preimplantational development in chemically defined media and IGF-I but not EGF may play a mitogenic role during early bovine development...
  45. ncbi Amino acid metabolism of preimplantation bovine embryos cultured with bovine serum albumin or polyvinyl alcohol
    Nicolas M Orsi
    Department of Biology, The University of York, PO Box 373, York YO10 5YW, UK
    Theriogenology 61:561-72. 2004
    ..These differences could partly be due to an increase in free intracellular amino acid concentration in SOFaaBSA embryos derived from hydrolysis of endocytosed BSA, and argue against the inclusion of PVA in bovine embryo culture media...
  46. ncbi Effect of protein supplementation on development to the hatching and hatched blastocyst stages of cat IVF embryos
    N W Kurniani Karja
    Department of Veterinary Sciences, Yamaguchi University, Japan
    Reprod Fertil Dev 14:291-6. 2002
    ..The results of the present study indicate that supplementation with FBS at different stages of early embryo development promotes development to the hatching and hatched blastocyst stages of cat IVF embryos...
  47. ncbi A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development
    N S Macklon
    Division of Reproductive Medicine, Department of Obstetrics and Gynaecology, Rotterdam, The Netherlands
    Hum Reprod 17:2700-5. 2002
    ..However, their improved efficacy over monoculture systems remains to be demonstrated in prospective studies...
  48. ncbi Effects of thioredoxin on the preimplantation development of bovine embryos
    Y Z Bing
    National Agricultural Research Center for Tohoku Region, Morioka, Japan
    Theriogenology 59:863-73. 2003
    ....
  49. ncbi Use of two replacements of serum during bovine embryo culture in vitro
    Paloma Duque
    Consejería de Medio Rural y Pesca, SERIDA CENSYRA, Camino de los Claveles 604, Somio, 33203 Gijón, Asturias, Spain
    Theriogenology 59:889-99. 2003
    ..The concentration of supplement and the timing of its inclusion in culture markedly affected embryo development. The serum replacement CPSR-3 can supplement embryo culture with blastocyst rates and quality similar to those for serum...
  50. ncbi Effect of the volume of medium and number of oocytes during in vitro fertilization on embryo development in pigs
    Maria Antonia Gil
    Department of Animal Pathology, Veterinary Science, University of Murcia, Murcia E 30071, Spain
    Theriogenology 60:767-76. 2003
    ..These results indicate that when 2000 spermatozoa/oocyte were used, a low volume of IVF medium (0.1 ml) and the number of oocytes during IVF (30-50) can improve the in vitro embryo production in pigs...
  51. ncbi Granulocyte-macrophage colony-stimulating factor (GM-CSF) acts independently of the beta common subunit of the GM-CSF receptor to prevent inner cell mass apoptosis in human embryos
    Cecilia Sjoblom
    Fertilitetscentrum AB, 402 29 Göteborg, Sweden
    Biol Reprod 67:1817-23. 2002
    ..Together, these data indicate that GM-CSF regulates cell viability in human embryos and that this potentially occurs through a novel receptor mechanism that is independent of betac...
  52. ncbi New developments reproductive technologies in deer
    D K Berg
    Reproductive Technologies Group, AgResearch, Ltd, Ruakura Research Center, Private Bag 3123, Hamilton, New Zealand
    Theriogenology 59:189-205. 2003
    ..The process of developing an in vitro embryo production system for farmed red deer may serve as a beneficial model for the propagation of endangered cervine species...
  53. ncbi Optimization of culture medium for cloned bovine embryos and its influence on pregnancy and delivery outcome
    Y H Choi
    Department of Theriogenology, College of Veterinary Medicine, Seoul, South Korea
    Theriogenology 58:1187-97. 2002
    ..However, enhanced development in vitro might not directly contribute to improving pregnancy outcomes...
  54. ncbi Use of G1.2/G2.2 media for commercial bovine embryo culture: equivalent development and pregnancy rates compared to co-culture
    Michelle Lane
    Research and Development Department, Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, CO 80110, USA
    Theriogenology 60:407-19. 2003
    ..2/G2.2 could be used in a commercial OPU transfer program without any loss in the numbers of blastocysts produced or the numbers of pregnancies resulting following transfer from either fresh or cryopreserved blastocysts...
  55. ncbi The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx
    Chin Der Chen
    Department of Obstetrics and Gynecology, National Taiwan University College of Medicine and Hospital, Taipei, Taiwan
    Hum Reprod 17:128-33. 2002
    ....
  56. ncbi Vascular endothelial growth factor (VEGF) promotes the early development of bovine embryo in the presence of cumulus cells
    Hailing Luo
    Department of Animal Production, College of Animal Science and Technology, China Agricultural University, Beijing, China
    J Vet Med Sci 64:967-71. 2002
    ..05) in all groups. These results suggest that VEGF has a beneficial effect on the initial development of bovine embryo through surrounding cumulus cells...
  57. ncbi Promoting effect of beta-mercaptoethanol on in vitro development under oxidative stress and cystine uptake of bovine embryos
    M Takahashi
    Department of Animal and Grassland Research, National Agricultural Center for Kyushu Okinawa Region, Kumamoto 861 1192, Japan
    Biol Reprod 66:562-7. 2002
    ..These results indicate that beta-ME has a protective effect in embryo development against oxidative stress and that the effect of beta-ME is associated with the promotion of cystine uptake of low availability in embryos...
  58. ncbi Dynamic in vivo imaging of postimplantation mammalian embryos using whole embryo culture
    E A V Jones
    Department of Chemical Engineering, California Institute of Technology, Pasadena, California
    Genesis 34:228-35. 2002
    ..To highlight some of the strengths of this approach, we present time-lapse movies of embryonic turning, somite addition, closure of the neural tube, and fluorescent imaging of blood circulation in the yolk sac and embryo...
  59. ncbi Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis
    Ri Cheng Chian
    Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada H3A 1A1
    Reprod Biomed Online 4:127-32. 2002
    ..9%, 79.9% and 73.9%) and blastocyst formation (27.4%, 33.0% and 30.4%) in the three groups. These results indicate that the maturational and developmental competence of immature oocytes is not affected by the phase of folliculogenesis...
  60. pmc IGF-1/IGFBP-1 increases blastocyst formation and total blastocyst cell number in mouse embryo culture and facilitates the establishment of a stem-cell line
    Ta Chin Lin
    Department of Gynecology, Obstetrics, and Infertility, Kuo General Hospital, NO, 22, Section 2, Ming Sheng Road, Tainan, 70343, Taiwan
    BMC Cell Biol 4:14. 2003
    ....
  61. ncbi Effects of follicular size of cytoplast donor on the efficiency of cloning in cattle
    Jorge A Piedrahita
    Department of Veterinary Anatomy and Public Health, Texas A and M University, College Station, Texas 77843 4458, USA
    Mol Reprod Dev 61:317-26. 2002
    ..No differences in survival to term (2/13 in each group) were observed. These results indicate that cytoplasts from follicles of 1-3 and 6-12 mm in diameter are equally developmentally competent when used in a nuclear transfer procedure...
  62. ncbi Effects of granulosa coculture on in-vitro oocyte meiotic maturation within a putatively less competent murine model
    Boon Chin Heng
    Department of Obstetrics and Gynaecology, Faculty of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074
    Theriogenology 62:1066-92. 2004
    ..ECM gel had a detrimental effect on fertilization and developmental competence, even though it had no detrimental effect on meiotic maturation itself...
  63. ncbi Sequential assessment of individually cultured human embryos as an indicator of subsequent good quality blastocyst development
    E Neuber
    Yale Center for Reproductive Medicine and Infertility, Yale University School of Medicine, New Haven, CT, USA
    Hum Reprod 18:1307-12. 2003
    ..In this study, an investigation was made to determine if developmental markers on the day of fertilization (day 1) can predict good subsequent blastocyst development...
  64. ncbi Mouse embryos generated from frozen-thawed oocytes can successfully survive a second cryopreservation
    Ariel Revel
    In Vitro Fertilization Unit, Department of Obstetrics and Gynecology, Hadassah University Hospital, Ein Kerem, Jerusalem, Israel
    Hum Reprod 19:666-9. 2004
    ..To determine whether mouse embryos generated from frozen-thawed oocytes can successfully survive a second cryopreservation...
  65. ncbi Selection of prepubertal goat oocytes using the brilliant cresyl blue test
    E Rodriguez-Gonzalez
    Departament de Ciencia Animal i dels Aliments, Facultat de Veterinaria, Universitat Autonoma de Barcelona, Spain
    Theriogenology 57:1397-409. 2002
    ..05) in the group of BCB+ (41.3 and 12.0%, respectively) than in BCB- oocytes (21.3 and 3.6%, respectively). In conclusion, the BCB test is a useful way to select more competent prepubertal goat oocytes for in vitro embryo production...
  66. ncbi Exposure to androgens during in vitro maturation does not affect the developmental potential of porcine oocytes
    J R Herrick
    Department of Animal Sciences, The Ohio State University, Columbus 43210, USA
    Theriogenology 58:1131-9. 2002
    ..20) between oocytes exposed to androgens and oocytes not exposed to androgens. These results indicate that exposure to androgens during IVM does not affect the ability of oocytes to cleave or develop up to the blastocyst stage in vitro...
  67. ncbi Preimplantation and postimplantation development of rat embryos cloned with cumulus cells and fibroblasts
    P Iannaccone
    Department of Pediatrics, Northwestern University Medical School and Children's Memorial Institute for Education and Research, Chicago, Illinois 60614, USA
    Zygote 9:135-43. 2001
    ..These results suggest that the nuclei of cultured rat cells, even following genetic modification, can be reprogrammed to support early embryonic development, which is a prerequisite to cloning the rat...
  68. ncbi Vitrification of rat embryos at various developmental stages
    M S Han
    The Graduate School of Natural Science and Technology, Okayama University, Okayama 700 8530, Japan
    Theriogenology 59:1851-63. 2003
    ..These results show that rat embryos at the 2-cell to blastocyst stages can be vitrified with EFS40, and that the morula stage is the most feasible stage for embryo cryopreservation in this species...
  69. ncbi Expression and role of the ether-à-go-go-related (MERG1A) potassium-channel protein during preimplantation mouse development
    N J Winston
    Department of Physiology and Institute for Biomedical Research, School of Biomedical Sciences, University of Sydney, New South Wales 2006, Australia
    Biol Reprod 70:1070-9. 2004
    ..These results show that MERG1A is developmentally regulated and suggest that it might play a role in early mouse embryogenic development...
  70. ncbi In vitro development and mitochondrial fate of macaca-rabbit cloned embryos
    Cai Xia Yang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
    Mol Reprod Dev 65:396-401. 2003
    ....
  71. ncbi Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression
    D Rizos
    Department of Animal Science and Production, University College Dublin, Lyons Research Farm, Newcastle, County Dublin, Ireland
    Biol Reprod 68:236-43. 2003
    ..The challenge now is to try and bridge this gap...
  72. ncbi Evaluation of mouse preimplantation embryos exposed to oxidative stress cultured with insulin-like growth factor I and II, epidermal growth factor, insulin, transferrin and selenium
    Rafał Kurzawa
    Clinic for Reproduction and Gynecology, Pomeranian Academy of Medicine, Szczecin, Poland
    Reprod Biol 2:143-62. 2002
    ..IGF-I, IGF-II, EGF and ITS have a positive effect on pre-implantation embryo development in detrimental culture conditions of oxidative stress...
  73. ncbi The effect of extracellular matrix molecules on mouse preimplantation embryo development in vitro
    F Figueiredo
    Monash Institute of Reproduction and Development, Monash University, Monash Medical Centre, 246 Clayton Road, Clayton, Victoria 3168, Australia
    Reprod Fertil Dev 14:443-51. 2002
    ..Heparan sulfate proteoglycan increases inner cell mass numbers and this may be due to interactions with the growth factors fibroblast growth factor 4 (FGF-4) and granulocyte-macrophage colony-stimulating factor...
  74. ncbi Protection against reactive oxygen species during mouse preimplantation embryo development: role of EDTA, oxygen tension, catalase, superoxide dismutase and pyruvate
    N M Orsi
    Department of Biology, University of York, York, United Kingdom
    Mol Reprod Dev 59:44-53. 2001
    ..The results suggest that embryo culture in EDTA-free medium under 5% O2 provides the most practical and physiological conditions for in vitro murine embryo culture...
  75. ncbi Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo HCG priming
    Weon Young Son
    In Vitro Fertilization Laboratory, Maria Infertility Hospital, Dongdaemun Gu, Seoul, Korea
    Hum Reprod 17:134-6. 2002
    ..This approach opens a potential for a new dimension in the management of patients with PCOS...
  76. ncbi Silicone oil used in microdrop culture can affect bovine embryonic development and freezability
    A Van Soom
    Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
    Reprod Domest Anim 36:169-76. 2001
    ..It appears that due to the changed lipid contents of the medium, embryonic membranes are rendered more susceptible to freezing due to altered membrane composition or by membrane damage caused by lipid peroxidation...
  77. ncbi Causes of developmental failure of in-vitro matured rhesus monkey oocytes: impairments in embryonic genome activation
    R Dee Schramm
    Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
    Hum Reprod 18:826-33. 2003
    ....
  78. ncbi Nuclear-cytoplasmic "tug of war" during cloning: effects of somatic cell nuclei on culture medium preferences of preimplantation cloned mouse embryos
    Young Gie Chung
    The Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 North Broad Street, Philadelphia, PA 19140, USA
    Biol Reprod 66:1178-84. 2002
    ..The relevance of this to understanding the kinetics and mechanisms of nuclear reprogramming and to the eventual improvement of the overall success in cloning is discussed...
  79. ncbi Overnight incubation improves selection of frozen-thawed blastocysts for transfer: preliminary study using supernumerary embryos
    Fabrice Guerif
    Physiopathologie de la Reproduction, Unité Mixte de Recherche 6073, Physiologie de la Reproduction et des Comportements, INRA CNRS Université de Tours, CHU Bretonneau, 37044 Tours Cedex, France
    Theriogenology 60:1457-66. 2003
    ..Increasing the interval between thawing and transfer appears to be beneficial in order to better select for transfer frozen-thawed blastocysts...
  80. ncbi Efects of the pine needle abortifacient, isocupressic acid, on bovine oocyte maturation and preimplantation embryo development
    S Wang
    USDA ARS, US Sheep Experiment Station, Dubois, ID 83423, USA
    Anim Reprod Sci 81:237-44. 2004
    ..In conclusion, ICA showed no adverse effects on oocyte maturation and preimplantation embryo development in vitro or subsequent viability in vivo using the ICA concentrations and in vitro culture parameters of this study...
  81. ncbi A prospective trial of blastocyst culture and transfer
    Takafumi Utsunomiya
    St Luke Clinic, 5 Tsumori Tomioka, Oita City 870 0947, Japan
    Hum Reprod 17:1846-51. 2002
    ..The aim of this study was to evaluate the effectiveness of the blastocyst culture method compared with the conventional day 3 transfer method using a prospective trial...
  82. ncbi Ultrastructural morphometry of bovine blastocysts produced in vivo or in vitro
    A E Crosier
    Departments of Animal Science, North Carolina State University, Raleigh 27695-7621, USA
    Biol Reprod 64:1375-85. 2001
    ..However, the specific nature of these deviations varied with the type of culture conditions used for in vitro embryo production...
  83. ncbi In vitro culture and interferon-tau secretion by ovine blastocysts
    W Caroline Lo
    Research Institute for Animal Production, Bogor, Indonesia, Australia
    Anim Reprod Sci 70:191-202. 2002
    ..The mean concentration of IFN-tau secreted by day-8 embryos after 1 day in culture (10.99+/-2.55 ng/ml) was not significantly different to day-7 embryos after 2 days in culture (8.8+/-1.75 ng/ml)...
  84. ncbi Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification
    T L Nedambale
    Connecticut Center for Regenerative Biology, University of Connecticut, 1392 Storrs Rd, U 4233, Storrs, CT 06269 4223, USA
    Theriogenology 62:437-49. 2004
    ..Vitrification using VS3a may be used more effectively to cryopreserve in vitro produced embryos than the conventional slow freezing method...
  85. ncbi Improved cleavage of bovine ICSI ova cultured in heparin-containing medium
    Guang Peng Li
    Animal Reproduction and Biotechnology Laboratory, Foothills Campus, Colorado State University, Fort Collins, CO 80523, USA
    Theriogenology 61:1077-84. 2004
    ..05)...
  86. ncbi Apolipoprotein E receptors are required for reelin-induced proteasomal degradation of the neuronal adaptor protein Disabled-1
    Hans H Bock
    Department of Medicine II, Albert Ludwigs Universitat, Albertstrasse 23, 79104 Freiburg, Germany
    J Biol Chem 279:33471-9. 2004
    ....
  87. ncbi Blastocyst culture in human IVF: the final destination or a stop along the way?
    A L Smith
    Fertility Lab Consulting, San Antonio, Texas 78230, USA
    Theriogenology 57:97-107. 2002
    ..Culture of human embryos to the blastocyst stage can be an effective method for improving implantation rates and reducing the high order multiple pregnancy rates seen in human IVF clinics when more than three embryos are transferred...
  88. ncbi [Study on in vitro culture and cryopreservation by vitrification of blastocysts derived from single mouse 2-cell embryos' blastomeres]
    Q G Meng
    College of Animal Science, China Agricultural University, Beijing 100094
    Shi Yan Sheng Wu Xue Bao 34:329-31. 2001
    ..However, the survival rates increased to 64% and 70% when the blastocysts were vitrified(one-step method) ater equilibration in EFS40 at 25 degrees C for 0.5-1 min...
  89. ncbi Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst
    Woo Suk Hwang
    College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    Science 303:1669-74. 2004
    ..Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells...
  90. ncbi Bile acids in the assessment of hepatocellular function
    M B Thompson
    Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Toxicol Pathol 24:62-71. 1996
    ..Recent developments in culture techniques permit long-term maintenance of functionally stable, differentiated cells...
  91. pmc Soil-transmitted helminthiases: nationwide survey in China
    L Q Xu
    Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai
    Bull World Health Organ 73:507-13. 1995
    ..By stool examinations using the Kato-Katz thick-smear and larval-culture techniques, overall prevalences of 47.0%, 18.8%, and 17...
  92. ncbi [Is there a reliable new diagnostic procedure in mycobacterial infections including tuberculosis?]
    R Urbanczik
    Med Diagnost Institut, Schiffweiler
    Pneumologie 48:156-9. 1994
    ..monitor drug resistance rates (pulmonary TB) and with other forms of TB or other mycobacterioses radiometric culture techniques, particularly if combined with conventional egg media cultures and with identification of grown bacilli ..
  93. pmc New prospects for the diagnosis of viral infections
    R H Yolken
    Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Yale J Biol Med 62:131-9. 1989
    ..diagnostic assays is limited by the time, expense, and expertise required for the performance of tissue culture techniques. Similarly, the application of immunoassay techniques has been inhibited by the limited degrees of ..
  94. pmc Sexually transmitted viruses
    F Rapp
    Department of Microbiology and Immunology, Pennsylvania State University, College of Medicine, Hershey
    Yale J Biol Med 62:173-85. 1989
    ..human pathogens has been learned only recently, owing to the advent of DNA technologies and advances in culture techniques. In fact, our awareness of one virally transmitted venereal disease, acquired immunodeficiency syndrome, ..
  95. ncbi Maintenance of cell viability in stored bone
    N J Treble
    Royal Liverpool Hospital, University of Liverpool, England
    Spine (Phila Pa 1976) 15:830-2. 1990
    ..By the use of tissue culture techniques, the authors show that it is possible to keep bone cells alive for many weeks...
  96. ncbi [Comparison of amplified Mycobacterium Tuberculosis Direct Test (MTD), Amplicor Mycobacteria kit (Amplicor) and PCR method for detection Mycobacterium tuberculosis in clinical specimens]
    M Goto
    Department of Infectious Diseases, University of Tokyo
    Kansenshogaku Zasshi 69:539-45. 1995
    ..These were shown to be at least as sensitive as the conventional culture techniques. The Tokyo Univ. method using radioisotope, takes up to 3 days, on the other hand 2 kits take 4-5 hours...
  97. pmc The diagnosis of human immunodeficiency virus infection: progress in less than five years
    F J Michalski
    Diagnostic Virology Laboratory, MetPath, Inc, Teterboro, NJ 07608
    Yale J Biol Med 62:93-102. 1989
    ..period of time since then, we have witnessed improvements in antibody detection methods, refinements in culture techniques, and the introduction of antigen and nucleic acid detection methods, including the polymerase chain reaction...
  98. ncbi A transversally isotropic elasto-damage constitutive model for the periodontal ligament
    A N Natali
    Centre of Mechanics of Bilogical Materials, University of Padova, Via Marzolo 9, I 35131, Italy
    Comput Methods Biomech Biomed Engin 6:329-36. 2003
    ..A finite element analysis is presented in order to define a general numerical approach. A comparison of numerical and experimental data is provided in order to show the reliability and effectiveness of the formulation assumed...
  99. ncbi Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak trees
    I Hernandez
    Instituto Madrileño de Investigación Agraria y Alimentaria, Apdo 127, 28800 AlcalA de Henares, Madrid, Spain
    Plant Cell Rep 21:765-70. 2003
    ..Although the mean percentage of recovery for the whole process was low, plants could be regenerated from four of the five trees tested...
  100. ncbi Transfer of inflammatory cytokines across the placenta
    Michael V Zaretsky
    Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9032, USA
    Obstet Gynecol 103:546-50. 2004
    ..The purpose of this study was to determine whether the placental transfer of interleukin (IL)-1alpha, IL-6, and tumor necrosis factor-alpha (TNF-alpha) occurs...
  101. ncbi Pro- or anti-oxidant manganese: a suggested mechanism for reconciliation
    Diem HaMai
    Department of Community and Environmental Medicine, Center for Occupational and Environmental Health, University of California, Irvine, CA 92692 1820, USA
    Neurochem Int 44:223-9. 2004
    ....

Research Grants67

  1. Novel Determination Of Chondrocyte Material Properties
    CLARK HUNG; Fiscal Year: 2004
    ..Successful implementation of the aforementioned will permit new research questions regarding the role of biomechanical influences in the development and progression of OA to be addressed. ..
  2. Chondrocyte Mechanotransduction Using Microfluidics
    CLARK HUNG; Fiscal Year: 2009
    ....
  3. Intervertebral Disc Response to Cyclic Loading in Vivo
    CLARK HUNG; Fiscal Year: 2005
    ..abstract_text> ..
  4. Quantitating Absorption of Complex Chemical Mixtures
    Jim Riviere; Fiscal Year: 2009
    ..abstract_text> ..
  5. ANTI-VIRAL TREATMENT OF DEVELOPING BOVINE EMBRYOS
    Maurice Givens; Fiscal Year: 2002
    ....
  6. Intracellular signaling in anesthetic and hypoxic preconditioning of neurons
    Philip E Bickler; Fiscal Year: 2010
    ..The work will also identify why the aging brain may not be able to benefit from the neuroprotective actions of anesthetics or other preconditioning treatments. ..
  7. Quantitative Microfluidic Molecular Evolution
    BRIAN PAEGEL; Fiscal Year: 2006
    ....
  8. Role of the CD40/CD40L dyad in atherosclerosis
    Masanori Aikawa; Fiscal Year: 2006
    ..abstract_text> ..
  9. Regulation of lipolysis by nitric oxide and adenosine
    Robert Hickner; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  10. Assessment of Tissue Response to Stress
    Joan Sanders; Fiscal Year: 2006
    ..this technology could be applied to other areas of rehabilitation, for example, shoe design for patients with insensate feet, cushion design for wheelchair users, and mattress design for bedridden patients [unreadable] [unreadable]..
  11. Metabolism and Excitability in Rodent Hippocampus
    Anne Williamson; Fiscal Year: 2006
    ..abstract_text> ..
  12. Mechanism of cytochrome P450 uncoupling and inactivation
    JARED GOLDSTONE; Fiscal Year: 2005
    ..Recently identified AhR-agonistic persistent pollutants, such as polybrominated diphenyl ethers will also be investigated. ..
  13. GDNF Protection in the Inner Ear
    Yehoash Raphael; Fiscal Year: 2005
    ..The data we propose to generate will enhance our understanding of the mechanisms of action of GDNF in normal and injured inner ear epithelia, knowledge that may eventually lead to better clinical treatments ..
  14. Skin Adaptation to Mechanical Stress
    Joan Sanders; Fiscal Year: 2006
    ..By understanding the process of skin adaptation (natural skin strengthening) at both a cellular and molecular level, therapies to encourage skin adaptation before breakdown occurs can be intelligently pursued. ..
  15. Novel Design for Artificial Transcription Factors
    Dusan Stanojevic; Fiscal Year: 2006
    ..The results of this Phase I research may have important implications in pharmaceutical drug development and may provide new tools for basic research in molecular genetics. [unreadable] [unreadable] [unreadable]..
  16. Age-Dependent Response of Hippocampal Neurons to Stress
    Gregory Brewer; Fiscal Year: 2006
    ..abstract_text> ..
  17. C. Pneumoniae and atherosclerotic plaque destabilization
    Michael E Rosenfeld; Fiscal Year: 2010
    ..4. To determine whether exposure of young versus old mice to air pollution increases the susceptibility of the mice to C. pneumoniae infection and accelerates the development and progression of atherosclerosis. ..
  18. IMMUNOPATHOGENESIS OF PSORIASIS
    Brian Nickoloff; Fiscal Year: 2009
    ....
  19. 14th Biennial FASEB Summer Research Conference on Retinoids
    Susan Smith; Fiscal Year: 2008
    ..Its goal is to stimulate scientific exchange and new investigations that will provide needed insights into retinoid roles in disease prevention and treatment. [unreadable] [unreadable] [unreadable]..
  20. Neural Crest Genes in Craniofacial Development
    LAURA GAMMILL; Fiscal Year: 2008
    ..The long-term career goal is to run a laboratory doing basic research in neural crest and craniofacial development. [unreadable] [unreadable]..
  21. ECI Conference on Engineering Cell Biology II - The Cell in Context
    Linda Griffith; Fiscal Year: 2007
    ..This conference will also emphasize translation of basic research into industry application in tissue engineering and drug development. [unreadable] [unreadable] [unreadable]..
  22. Calcium Signaling & Prefrontal Deficits in Schizophrenia
    David McCormick; Fiscal Year: 2008
    ..abstract_text> ..
  23. INTERFACE MECHANICS, MATERIALS AND TISSUE RESPONSE
    Joan Sanders; Fiscal Year: 2007
    ..The development of methods to predict and overcome the detrimental effects of residual limb shape change and ultimately prevent soft-tissue breakdown will help to prevent secondary disability and morbidity in the amputee population. ..
  24. Susceptibility mechanism in alcoholic pancreatitis
    Herbert Gaisano; Fiscal Year: 2007
    ..Significance: These actions of alcohol on pancreatic acinar PM-Munc18c may be the 'sentinel' event that initiates the role of alcohol in pancreatic injury, and which could be therapeutically targeted. ..
  25. Novel Molecular Tools for Transsynaptic Circuit Analysis
    Haig Keshishian; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  26. CELLULAR MECHANISMS OF VISUAL CORTICAL FUNCTION
    DAVID A contact MCCORMICK; Fiscal Year: 2010
    ..Our studies will give fundamental information relevant to understanding not only vision, but also attention and memory. ..
  27. Proximal Tubule Transport Defect in Hyp Mice
    Michel Baum; Fiscal Year: 2007
    ..These studies will not only examine the pathogenesis of this disorder, but will directly examine if indomethacin potentially represents a novel therapy for this disease. ..
  28. PEDIATRIC NEPHROLOGY TRAINING
    Michel Baum; Fiscal Year: 2005
    ..Fellows also attend seminars in basic science departments. The research mentor, as well as the program director, closely supervise trainees to ensure that the goals of the training program are achieved. ..
  29. CRANIOFACIAL MORPHOGENESIS IN PRENATAL ALCOHOL EXPOSURE
    Susan Smith; Fiscal Year: 2005
    ..Results also may provide possible explanations for genetic-based variations in fetal responses to prenatal ethanol exposure. ..
  30. Dietary Cysteine:Conceptal Response to Oxidative Stress
    Craig Harris; Fiscal Year: 2002
    ....
  31. ESTROGEN STATUS AND THE FUNCTION OF CORONARY ARTERIES
    VIRGINIA MILLER; Fiscal Year: 2002
    ..The experiments of this proposal take such an approach using molecular to integrated whole animal physiology. ..
  32. STRUCTURE/FUNCTION OF SUBCORTICAL WHITE MATTER NEURONS
    Anne Williamson; Fiscal Year: 2002
    ..Given the possible involvement of these cells in a variety of neurological disorders, it is critical to characterize these cells completely. ..
  33. Abnormalities in Keratoconus Corneas
    MARIA KENNEY; Fiscal Year: 2003
    ..These studies will provide fundamental insights into KC pathogensis and may provide a basis for therapy. ..
  34. Exercise, muscle nutritive blood flow and eNOS in aging
    Robert Hickner; Fiscal Year: 2003
    ..abstract_text> ..
  35. Estrogen: Chrodroprotective or Destructive?
    Sally Frenkel; Fiscal Year: 2002
    ..The inconclusive and contradictory data from human and animal studies render the proposed studies highly significant. ..
  36. A POXVIRAL PROTEIN THAT INHIBITS CHEMOKINE FUNCTION
    Mitchell Krathwohl; Fiscal Year: 2003
    ..4) To determine the regions of MC148R that are important for blocking chemotaxis by constructing mutant proteins at both the amino terminus and the internal heparin binding site. ..
  37. Volume Regulatory Pathways in Cystic Fibrosis Mice
    Won Kyoo Cho; Fiscal Year: 2003
    ..abstract_text> ..
  38. MECHANISMS OF PROSTAGLANDIN INDUCED INTESTINAL REPAIR
    Anthony Blikslager; Fiscal Year: 2002
    ..These experiments will provide insight into the signaling pathways and mechanisms by which prostaglandins trigger repair of acutely-injured intestinal epithelium. ..
  39. METHOD FOR DISCOVERING NOVEL TRANSCRIPTIONAL REGULATORS
    Dusan Stanojevic; Fiscal Year: 2004
    ..2) Developing a robust and rapid assay for testing of ATFs in human cell culture lines. 3) Demonstration of the ability of ATFs to target selected endogenous human genes in tissue culture. ..
  40. Gene Expression Analysis in Microcaptured Retinal Cells
    Ruben Adler; Fiscal Year: 2005
    ..The potential impact of the identification of these molecules is clear, since they could offer new avenues for the treatment of these devastating diseases. ..
  41. MHC-Defined and Identical Twin Monkeys for Research
    Joseph Kemnitz; Fiscal Year: 2005
    ..Additionally, genetically identical macaques will have general utility in biomedical research, especially to the neuroscience community. ..
  42. PHENOTYPE AND FUNCTION OF SENESCENT KERATINOCYTES
    Brian Nickoloff; Fiscal Year: 2005
    ....
  43. MECHANISMS OF PRETERM LABOR: NF-KB ACTIVATION OF COX-2
    DOUGLAS KNISS; Fiscal Year: 2001
    ....
  44. INTERFACE MECHANICS, MATERIALS, AND TISSUE RESPONSE
    Joan Sanders; Fiscal Year: 2002
    ....
  45. Role of BDNF in development and maintenance of taste bu*
    LINDA BARLOW; Fiscal Year: 2002
    ..By using conditional, tissue specific knockouts, we will be able to separate the effects of elimination of BDNF in adulthood from its trophic effects already documented for the embryonic period. ..
  46. The Role of NO in the Regulation of Heart Contractility
    BEATA WOLSKA; Fiscal Year: 2004
    ..Our experiments provide new insights into the mechanisms and importance of NO (NOx) in alteration of cardiac contractility in physiological and pathological conditions. ..
  47. HPV DIFFERENTIATION DEPENDENT REPLICATION
    Craig Meyers; Fiscal Year: 2003
    ..abstract_text> ..
  48. Genetic Analysis of Papillomavirus Virion Morphogenesis
    Craig Meyers; Fiscal Year: 2009
    ....
  49. Is S-adenosyl Methionine a Nutrition: Genetic Link in AD?
    Thomas Shea; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  50. Diesel Exhaust and Atherosclerotic Plaque Stability
    Michael Rosenfeld; Fiscal Year: 2007
    ....
  51. Chemoprevention of Uterine Cancer Risk by Antioxidants
    Minoti Sharma; Fiscal Year: 2003
    ..The long-term goal of this proposal is to evaluate if the combination of Tam or estrogen with nontoxic antioxidants will cooperate to reduce uterine cancer risk associated with chronic exposure to Tam or estrogen. ..
  52. Solamere QLC100 Nipkow Spinning Disk Confocal Unit
    JONATHAN JAGGAR; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  53. Cell Death in Normal and Diseased Human Epidermis
    Brian Nickoloff; Fiscal Year: 2006
    ..This grant features a transition of experimental protocols beginning with conventional tissue culture techniques, and then moving to the use of living epidermal equivalents that contain stratified layers of KCs that can ..
  54. 3D Adipose Tissue Generation and Functional Verification
    Charles Patrick; Fiscal Year: 2004
    ..abstract_text> ..
  55. H. Pylory factors associated with peptic ulcer disease
    TIMOTHY COVER; Fiscal Year: 2006
    ..pylori factors that help to determine clinical outcome, and ultimately, may lead to advances in the treatment or prevention of H. pylori-associated human diseases. [unreadable] [unreadable]..
  56. DEFORMATION-DEPENDENT ENZYME CLEAVAGE OF COLLAGEN
    Peter Torzilli; Fiscal Year: 2005
    ..The findings from this study could fundamentally change our concepts of matrix enzyme function and the design of intervention approaches to include the effect the mechanical environment has on the ECM. ..
  57. RNAi Mediated Gene Disruption in Caenorhabditis Elegans
    Wayne Van Voorhies; Fiscal Year: 2006
    ..Such worms could identify candidate genes affecting processes that act "downstream" from the metabolic rate and provide valuable insights into the causes of aging and mechanisms by which it can be attenuated. ..
  58. NEW BIOMATERIALS TO MINIMIZE SCAR TISSUE FORMATION
    Joan Sanders; Fiscal Year: 2002
    ..e. soft-tissue complications that require surgical repair through use of a biomaterial. The new materials will prevent scar tissue formation and reduce the occurrence of secondary complications. ..
  59. TENDON FIBER-MATRIX MECHANICS--THEORY AND EXPERIMENTS
    Dawn Elliott; Fiscal Year: 2003
    ..This information will enable us to develop and assess potential treatment modalities. ..
  60. Regulation of Pancreas Development by P13K/Akt Signaling
    Ernesto Bernal Mizrachi; Fiscal Year: 2006
    ..abstract_text> ..
  61. Role of SOCS in Regulation of Transplantation Tolerance
    Stanislaw Stepkowski; Fiscal Year: 2004
    ..Such an approach will directly evaluate whether Stat5 or Stat6 activation is regulated by SOCS3 and/or other SOCS. These experiments will provide the first evidence for the role of SOCS in induction of transplantation tolerance. ..
  62. Regulation of IL-4/IL-4R Signaling Pathway Mediate Tole*
    Stanislaw Stepkowski; Fiscal Year: 2003
    ..This study may provide evidence that tolerogenic vaccines induce potent Jak/Stat regulatory proteins, which are instrumental in tolerance induction. ..
  63. Characterizing Tissue Development Under Biaxial Loading
    Alvin Yeh; Fiscal Year: 2006
    ..This novel experimental platform will form the basis for novel and innovative studies in mechanobiology. ..
  64. QUANTITATIVE MICROBIOLOGIC MODEL FOR PRETERM DELIVERY
    Andrew Onderdonk; Fiscal Year: 2004
    ....