tissue fixation

Summary

Summary: The technique of using FIXATIVES in the preparation of cytologic, histologic, or pathologic specimens for the purpose of maintaining the existing form and structure of all the constituent elements.

Top Publications

  1. pmc Gene expression in fixed tissues and outcome in hepatocellular carcinoma
    Yujin Hoshida
    Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02142, USA
    N Engl J Med 359:1995-2004. 2008
  2. pmc Formalin fixation at low temperature better preserves nucleic acid integrity
    Gianni Bussolati
    Department of Biomedical Sciences and Human Oncology, University of Turin, Turin, Italy
    PLoS ONE 6:e21043. 2011
  3. ncbi RNA expression analysis from formalin fixed paraffin embedded tissues
    Susan M Farragher
    Almac Diagnostics, Craigavon, 19 Seagoe Industrial Estate, Armagh BT635QD, UK
    Histochem Cell Biol 130:435-45. 2008
  4. pmc Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples
    Craig April
    Illumina, Inc, San Diego, California, United States of America
    PLoS ONE 4:e8162. 2009
  5. pmc The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?
    M Thomas P Gilbert
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona, United States of America
    PLoS ONE 2:e537. 2007
  6. ncbi Generation of high-quality protein extracts from formalin-fixed, paraffin-embedded tissues
    Maria Filippa Addis
    Porto Conte Ricerche Srl, Tramariglio, Alghero, Sassari, Italy
    Proteomics 9:3815-23. 2009
  7. pmc Determinants of RNA quality from FFPE samples
    Silke von Ahlfen
    QIAGEN GmbH, Hilden, Germany
    PLoS ONE 2:e1261. 2007
  8. pmc Gene expression profiles from formalin fixed paraffin embedded breast cancer tissue are largely comparable to fresh frozen matched tissue
    Lorenza Mittempergher
    Divisions of Experimental Therapy, Molecular Biology, Surgical Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS ONE 6:e17163. 2011
  9. ncbi Automated extraction of DNA and RNA from a single formalin-fixed paraffin-embedded tissue section for analysis of both single-nucleotide polymorphisms and mRNA expression
    Guido Hennig
    Siemens Healthcare Diagnostics Products, Molecular Research Germany, Cologne, Germany
    Clin Chem 56:1845-53. 2010
  10. pmc Limited copy number-high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies
    Hongdo Do
    Department of Pathology, University of Melbourne, Parkville, Victoria, 3010, Australia
    Mol Cancer 8:82. 2009

Detail Information

Publications259 found, 100 shown here

  1. pmc Gene expression in fixed tissues and outcome in hepatocellular carcinoma
    Yujin Hoshida
    Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02142, USA
    N Engl J Med 359:1995-2004. 2008
    ..An obstacle to the development of genome-based predictors of outcome in patients with hepatocellular carcinoma has been the lack of a means to carry out genomewide expression profiling of fixed, as opposed to frozen, tissue...
  2. pmc Formalin fixation at low temperature better preserves nucleic acid integrity
    Gianni Bussolati
    Department of Biomedical Sciences and Human Oncology, University of Turin, Turin, Italy
    PLoS ONE 6:e21043. 2011
    ..that RNA fragmentation is caused by activation of RNAses during the interval between formalin penetration and tissue fixation. To prevent RNAse activation, a series of tissue samples were kept under-vacuum at 4°C until fixation and ..
  3. ncbi RNA expression analysis from formalin fixed paraffin embedded tissues
    Susan M Farragher
    Almac Diagnostics, Craigavon, 19 Seagoe Industrial Estate, Armagh BT635QD, UK
    Histochem Cell Biol 130:435-45. 2008
    ..This paper reviews the progress in this field and discusses the unique opportunities that exist for the application of these techniques in the development of personalized medicine...
  4. pmc Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples
    Craig April
    Illumina, Inc, San Diego, California, United States of America
    PLoS ONE 4:e8162. 2009
    ..We have developed a gene expression assay (Whole-Genome DASL), capable of generating whole-genome gene expression profiles from degraded samples such as formalin-fixed, paraffin-embedded (FFPE) specimens...
  5. pmc The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?
    M Thomas P Gilbert
    Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona, United States of America
    PLoS ONE 2:e537. 2007
    ..Our findings recommend that the extraction methodology adopted should be chosen carefully. Here we provide a quick reference table that can be used to determine appropriate protocols for particular aims...
  6. ncbi Generation of high-quality protein extracts from formalin-fixed, paraffin-embedded tissues
    Maria Filippa Addis
    Porto Conte Ricerche Srl, Tramariglio, Alghero, Sassari, Italy
    Proteomics 9:3815-23. 2009
    ..An evaluation of the extent of modifications introduced on proteins by formalin fixation and crosslink reversal, and their impact on quality of MS results, is also reported...
  7. pmc Determinants of RNA quality from FFPE samples
    Silke von Ahlfen
    QIAGEN GmbH, Hilden, Germany
    PLoS ONE 2:e1261. 2007
    ..The results are particularly relevant for any application relying on cDNA synthesis as an initial step of the procedure, such as RT-PCR, and microarray analysis...
  8. pmc Gene expression profiles from formalin fixed paraffin embedded breast cancer tissue are largely comparable to fresh frozen matched tissue
    Lorenza Mittempergher
    Divisions of Experimental Therapy, Molecular Biology, Surgical Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS ONE 6:e17163. 2011
    ..To this end we profiled, in duplicate, 20 FFPE tissues and 20 matched FF tissues and evaluated the concordance of the DASL results from FFPE and matched FF material...
  9. ncbi Automated extraction of DNA and RNA from a single formalin-fixed paraffin-embedded tissue section for analysis of both single-nucleotide polymorphisms and mRNA expression
    Guido Hennig
    Siemens Healthcare Diagnostics Products, Molecular Research Germany, Cologne, Germany
    Clin Chem 56:1845-53. 2010
    ..We investigated a fully automated, xylene-free nucleic acid extraction method for the simultaneous analysis of RNA and DNA biomarkers related to breast cancer...
  10. pmc Limited copy number-high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies
    Hongdo Do
    Department of Pathology, University of Melbourne, Parkville, Victoria, 3010, Australia
    Mol Cancer 8:82. 2009
    ..The dilutions used (based on Ct values) are chosen such that mutations, if present, can be detected by the direct sequencing of amplicons with aberrant melting patterns...
  11. pmc Application of a BRAF pyrosequencing assay for mutation detection and copy number analysis in malignant melanoma
    Cynthia Spittle
    Clinical Translational Medicine, Oncology, Wyeth Research, 500 Arcola Rd, Collegeville, PA 19426, USA
    J Mol Diagn 9:464-71. 2007
    ..Pyro-sequencing serves as an excellent platform for BRAF genotyping of tumors from patients entering clinical trial...
  12. pmc Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples
    Yaguang Xi
    Cancer Genomics Laboratory, Mitchell Cancer Institute, Mobile, Alabama 36688, USA
    RNA 13:1668-74. 2007
    ..There are no significant differences of representative miRNA expression among 40 colorectal cancer FFPE specimens. This study provides a foundation for miRNA investigation using FFPE samples in cancer and other types of diseases...
  13. pmc Histological assessment of PAXgene tissue fixation and stabilization reagents
    Marcel Kap
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC, Rotterdam, The Netherlands
    PLoS ONE 6:e27704. 2011
    ..It is our aim to assess the suitability of PAXgene tissue fixation for (immuno)histological methods...
  14. pmc The HOPE fixation technique--a promising alternative to common prostate cancer biobanking approaches
    Martin Braun
    Institute of Prostate Cancer Research, Institute of Pathology, University Hospital Bonn, Sigmund Freud Strasse 25, 53127 Bonn, Germany
    BMC Cancer 11:511. 2011
    ..Aim of the study was to compare HOPE-fixed, FFPE and fresh-frozen bio-specimens for their accessibility for diagnostic and research purposes...
  15. ncbi DNA extraction from archival formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval principle: heating under the influence of pH
    Shan Rong Shi
    Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Histochem Cytochem 50:1005-11. 2002
    ..These findings may lead to a more effective protocol for DNA extraction from archival paraffin-embedded tissues and may also provide enhanced understanding of changes that occur during formalin-induced modification of nucleic acids...
  16. pmc A multiplex PCR predictor for aCGH success of FFPE samples
    E H van Beers
    Division of Experimental Therapy, Netherlands Cancer Institute NKI AvL, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Br J Cancer 94:333-7. 2006
    ....
  17. ncbi Quantitative protein analysis from formalin-fixed tissues: implications for translational clinical research and nanoscale molecular diagnosis
    K F Becker
    Technische Universitat, Institut fur Pathologie, Munchen, Germany
    J Pathol 211:370-8. 2007
    ..This technique may also be used to identify, characterize, and validate known and new protein markers in a variety of human diseases...
  18. ncbi Proteomic analysis of formalin-fixed prostate cancer tissue
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
    Mol Cell Proteomics 4:1741-53. 2005
    ....
  19. ncbi Comparison of gene expression profiling by reverse transcription quantitative PCR between fresh frozen and formalin-fixed, paraffin-embedded breast cancer tissues
    Iker Sánchez-Navarro
    Laboratory of Molecular Pathology and Oncology, Research Unit, Hospital Universitario La Paz, Madrid, Spain
    Biotechniques 48:389-97. 2010
    ..Our results could serve as a guide when developing clinical diagnostic tests based on RT-qPCR analyses of FFPE tissues in the coming era of treatment decision-making based on gene expression profiling...
  20. ncbi Relative indices of water diffusion anisotropy are equivalent in live and formalin-fixed mouse brains
    Shu Wei Sun
    Department of Radiology, Washington University School of Medicine, St Louis, Missouri
    Magn Reson Med 50:743-8. 2003
    ..The combination of DTI measurements and standard histology may shed light on the microstructural determinants of diffusion anisotropy in normal and disease states...
  21. pmc Quantitative expression profiling in formalin-fixed paraffin-embedded samples by affymetrix microarrays
    Diana Abdueva
    Department of Pathology, Children s Hospital Los Angeles, Research Institute and Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    J Mol Diagn 12:409-17. 2010
    ..This study provides guidelines for application of these methods in the discovery, validation, and clinical application of microarray expression profiling with FFPE material...
  22. pmc KRAS mutation: comparison of testing methods and tissue sampling techniques in colon cancer
    Wilbur A Franklin
    Department of Pathology, University of Colorado Denver, Fitzsimons Campus, 12801 E 17 Ave, Aurora, CO 80045, USA
    J Mol Diagn 12:43-50. 2010
    ..We conclude that coring of the paraffin blocks and testing by ARMS/S is a sensitive, specific, and efficient method for KRAS testing...
  23. pmc A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections
    Hiroaki Nitta
    Medical Innovation, Ventana Medical Systems, Inc, Tucson, AZ, USA
    Diagn Pathol 7:60. 2012
    ....
  24. ncbi Standardization in immunohistology
    Anthony S Y Leong
    University of Newcastle, Newcastle, NSW, Australia
    Methods Mol Biol 724:37-68. 2011
    ..b>Tissue fixation is pivotal to antigen preservation but exposure to fixative prior to accessioning by the laboratory is not ..
  25. ncbi Effect of ischemic time, fixation time, and fixative type on HER2/neu immunohistochemical and fluorescence in situ hybridization results in breast cancer
    Neda A Moatamed
    Dept of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California Los Angeles, 10833 Le Conte Ave CHS, Los Angeles, CA 90095, USA
    Am J Clin Pathol 136:754-61. 2011
    ..Our study demonstrates that HER2/neu results remain accurate beyond ASCO/CAP-recommended preanalytic variables, with the exception of Bouin solution for FISH analysis...
  26. ncbi The use of formalin fixed wax embedded tissue for proteomic analysis
    Lynda D Ralton
    Department of Pathology, University of Aberdeen, Aberdeen, UK
    J Clin Pathol 64:297-302. 2011
    ..The purpose of this review is to provide an overview of the issues associated with the use of formalin fixed wax embedded tissues for proteomics...
  27. pmc Extreme loss of immunoreactive p-Akt and p-Erk1/2 during routine fixation of primary breast cancer
    Isabel F Pinhel
    Department of Academic Biochemistry, Institute of Cancer Research, London, UK
    Breast Cancer Res 12:R76. 2010
    Very few studies have investigated whether the time elapsed between surgical resection and tissue fixation or the difference between core-cut and excision biopsies impact on immunohistochemically measured biomarkers including ..
  28. pmc Genome-wide expression analysis of recently processed formalin-fixed paraffin embedded human prostate tissues
    Thomas A Dunn
    Department of Urology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Prostate 69:214-8. 2009
    ..However, the overall impact of the FFPE procedure alone on the reliability and accuracy of expression data, without influences from additional compromising factors introduced during long-term storage, has not been vigorously assessed...
  29. pmc KRAS analysis in colorectal carcinoma: analytical aspects of Pyrosequencing and allele-specific PCR in clinical practice
    Magnus Sundstrom
    Molecular Pathology Unit, Department of Genetics and Pathology, Uppsala University, SE 751 85 Uppsala, Sweden
    BMC Cancer 10:660. 2010
    ..Several procedures to detect KRAS mutations have been developed. However, the analytical sensitivity and specificity of these assays on routine clinical samples are not yet fully characterised...
  30. ncbi Quantitative DNA methylation analysis of laser capture microdissected formalin-fixed and paraffin-embedded tissues
    Jean Francois Gagnon
    Service de Pathologie, Centre Hospitalier Affilie Universitaire De Quebec, Quebec, Canada
    Exp Mol Pathol 88:184-9. 2010
    ..Our data demonstrate that this assay based on digestion with MSRE and qPCR procedures is a good technique to quantify MP on limited amounts of DNA and may find clinical applications...
  31. ncbi Whole genome SNP arrays using DNA derived from formalin-fixed, paraffin-embedded ovarian tumor tissue
    Ella R Thompson
    VBCRC Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
    Hum Mutat 26:384-9. 2005
    ..This advance will open up vast archival tissue resources to high-resolution genetic analysis and unlock a wealth of biological information...
  32. ncbi Evaluation of ethanol-fixed, paraffin-embedded tissues for proteomic applications
    Mamoun Ahram
    Pathogenetics Unit, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Proteomics 3:413-21. 2003
    ..In light of these results, we conclude that ethanol-fixed tissues can be successfully utilized for proteomic analyses...
  33. pmc Diffusion imaging of whole, post-mortem human brains on a clinical MRI scanner
    Karla L Miller
    FMRIB Centre, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, UK
    Neuroimage 57:167-81. 2011
    ..Despite these alterations, diffusion tractography of several major tracts is successfully demonstrated at both resolutions. We also report novel findings of cortical anisotropy and partial volume effects...
  34. pmc Evaluation and validation of total RNA extraction methods for microRNA expression analyses in formalin-fixed, paraffin-embedded tissues
    Martina Doleshal
    Asuragen, Inc, Austin, TX 78744, USA
    J Mol Diagn 10:203-11. 2008
    ....
  35. ncbi DNA extraction from archival formalin-fixed, paraffin-embedded tissues: heat-induced retrieval in alkaline solution
    Shan Rong Shi
    Department of Pathology, University of Southern California Keck School of Medicine HMR 204, 2011 Zonal Avenue, Los Angeles 90033, USA
    Histochem Cell Biol 122:211-8. 2004
    ..1 M alkaline solution is a simpler and more effective heat-induced retrieval protocol for DNA extraction. Combination with some chemicals (detergents) may further significantly improve efficiency of the heat-induced retrieval technique...
  36. ncbi MicroRNA profiling of clear cell renal cell carcinoma by whole-genome small RNA deep sequencing of paired frozen and formalin-fixed, paraffin-embedded tissue specimens
    Lihong Weng
    Department of Pathology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    J Pathol 222:41-51. 2010
    ..To our knowledge, this is the first study to demonstrate that FFPE specimens can be used reliably for miRNA deep-sequencing analysis, making future large-scale clinical cohort/trial-based studies possible...
  37. pmc Multicentre validation study of nucleic acids extraction from FFPE tissues
    Serena Bonin
    ACADEM Department University of Trieste and ICGEB, University of Trieste, Cattinara Hospital, 34149 Trieste, Italy
    Virchows Arch 457:309-17. 2010
    ..The results of the study emphasize the need for quality control of the nucleic acid extracts with standardised methods to prevent false negative results and to allow data comparison among different diagnostic laboratories...
  38. pmc An array-based analysis of microRNA expression comparing matched frozen and formalin-fixed paraffin-embedded human tissue samples
    Xiao Zhang
    Department of Pathology and Molecular Medicine, Queen s Laboratory for Molecular Pathology, Kingston, ON, Canada
    J Mol Diagn 10:513-9. 2008
    ..669 to 0.815; Spearman rank correlation coefficient, 0.847 to 0.948), supporting the use of FFPE-derived miRNAs for array-based, gene expression profiling...
  39. ncbi Analytical performance of a real-time PCR-based assay for V600 mutations in the BRAF gene, used as the companion diagnostic test for the novel BRAF inhibitor vemurafenib in metastatic melanoma
    Harkanwal Halait
    Roche Molecular Diagnostics, Pleasanton, CA 94588, USA
    Diagn Mol Pathol 21:1-8. 2012
    ..A simple 1:2 dilution resulted in a valid test result of 76% in such cases. The cobas test is a reproducible assay that detects some non-V600E mutations and is more accurate than direct sequencing in detecting BRAFV600E...
  40. ncbi Antigen retrieval techniques: current perspectives
    S R Shi
    Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Histochem Cytochem 49:931-7. 2001
    ..Furthermore, understanding the mechanism of the AR technique may shed light on facilitating the development of molecular morphology...
  41. ncbi 'Tissue surrogates' as a model for archival formalin-fixed paraffin-embedded tissues
    Carol B Fowler
    Department of Biophysics, Armed Forces Institute of Pathology, Rockville, MD 20850, USA
    Lab Invest 87:836-46. 2007
    ....
  42. ncbi Protein unlocking procedures of formalin-fixed paraffin-embedded tissues: application to MALDI-TOF imaging MS investigations
    Maurizio Ronci
    Centro Studi Sull Invecchiamento, Chieti, Italy
    Proteomics 8:3702-14. 2008
    ..A multivariate statistical evaluation is presented and discussed according to molecular spatial distributions and tissue morphology...
  43. pmc Analyses and interpretation of whole-genome gene expression from formalin-fixed paraffin-embedded tissue: an illustration with breast cancer tissues
    Muhammad G Kibriya
    Department of Health Studies, The University of Chicago, 5841 S, Maryland Avenue, MC 2007, Chicago, IL 60637, USA
    BMC Genomics 11:622. 2010
    ..We used a whole genome DASL assay (addressing 18,391 genes) on a total of 72 samples from paired breast tumor and surrounding healthy tissues from both FF and FFPE samples...
  44. pmc KRAS genotyping of paraffin-embedded colorectal cancer tissue in routine diagnostics: comparison of methods and impact of histology
    Wilko Weichert
    Institut fur Pathologie, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    J Mol Diagn 12:35-42. 2010
    ....
  45. ncbi Formalin fixation alters water diffusion coefficient magnitude but not anisotropy in infarcted brain
    Shu Wei Sun
    Department of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Magn Reson Med 53:1447-51. 2005
    ..However, the sensitivity of water diffusion in detecting tissue injury in vivo is not preserved in fixed tissues...
  46. ncbi Methods comparison for high-resolution transcriptional analysis of archival material on Affymetrix Plus 2.0 and Exon 1.0 microarrays
    Kim Linton
    Cancer Research UK Department of Medical Oncology, the Christie NHS Foundation Trust, Manchester, UK
    Biotechniques 47:587-96. 2009
    ..While larger series are needed to confirm high correspondence between fresh-frozen and FFPE expression patterns, these data suggest that both Plus 2.0 and Exon arrays are suitable platforms for FFPE microarray expression analyses...
  47. ncbi Metastatic colorectal cancer KRAS genotyping in routine practice: results and pitfalls
    Aude Lamy
    Laboratory of Tumor Genetics, University Hospital, Rouen, France
    Mod Pathol 24:1090-100. 2011
    ..The tumor heterogeneity observed in some patients indicates that it should be more appropriate to perform KRAS genotyping on metastases if sample is available...
  48. ncbi EGFR and K-ras mutation analysis in non-small cell lung cancer: comparison of paraffin embedded versus frozen specimens
    Marielle I Gallegos Ruiz
    Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands
    Cell Oncol 29:257-64. 2007
    ..Furthermore, we compare the sensitivity of the analysis using genomic DNA (gDNA) versus RNA...
  49. ncbi Mass spectrometric analysis of formalin-fixed paraffin-embedded tissue: unlocking the proteome within
    Brian L Hood
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
    Proteomics 6:4106-14. 2006
    ..Although these developments are quite new, the ability to conduct MS-based proteomic analyses of FFPE tissues opens heretofore intractable clinical samples for discovery-based biomarker research...
  50. ncbi Efficient method for the proteomic analysis of fixed and embedded tissues
    Darryl Erik Palmer-Toy
    Harvard Partners Center for Genetics and Genomics, Cambridge, Massachusetts, USA
    J Proteome Res 4:2404-11. 2005
    ..Moreover, we demonstrate that a high level of sequence coverage can be observed for proteins of interest...
  51. pmc Evaluation of non-formalin tissue fixation for molecular profiling studies
    John W Gillespie
    Pathogenetics Unit, Laboratory of Pathology, National Cancer Institute, Rm 2C500 Bldg 10, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Am J Pathol 160:449-57. 2002
    ..Complete protocols and additional discussion of relevant issues are available on an accompanying website (http://cgap-mf.nih.gov/)...
  52. pmc Effect of fixatives and tissue processing on the content and integrity of nucleic acids
    Mythily Srinivasan
    Department of Pathology, Tzagournis Medical Research Facility, The Ohio State University, 420 West 12th Avenue, Columbus, OH 43210, USA
    Am J Pathol 161:1961-71. 2002
    ..In addition, the parameters of procurement and fixation that affect the quality of the tissues at the molecular level are discussed...
  53. ncbi The challenge and importance of standardizing pre-analytical variables in surgical pathology specimens for clinical care and translational research
    D G Hicks
    Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, 601 Elmwood Avenue, Box 626, Rochester, NY 14642, USA
    Biotech Histochem 87:14-7. 2012
    ..Inaccurate results owing to compromised tissue quality can lead to false positive or false negative results with therapeutic consequences that can harm patients and affect their eventual outcome...
  54. ncbi Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues
    John B A Okello
    McMaster Ancient DNA Centre, Department of Anthropology, McMaster University, Hamilton, Ontario L8S4L9, Canada
    Anal Biochem 400:110-7. 2010
    ....
  55. ncbi The effect of tissue fixation and processing on breast cancer size
    Bobbi Pritt
    Department of Pathology, University of Vermont College of Medicine, Burlington, VT 05405, USA
    Hum Pathol 36:756-60. 2005
    ..One can arguably measure the size of an invasive breast cancer from either the fresh or fixed state without affecting accuracy, but caution should be exercised in relying solely on the microscopic measurements...
  56. ncbi Setting proteins free: progresses and achievements in proteomics of formalin-fixed, paraffin-embedded tissues
    Alessandro Tanca
    Porto Conte Ricerche, Tramariglio, Alghero, Italy
    Proteomics Clin Appl 6:7-21. 2012
    ..In conclusion, the future perspectives and open challenges in this field are discussed...
  57. ncbi Optimization of recovery of RNA from formalin-fixed, paraffin-embedded tissue
    Joon Yong Chung
    Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4605, USA
    Diagn Mol Pathol 15:229-36. 2006
    ..Pretreatment with RNA, later followed by formalin fixation, did not result in improving the RNA quality, but did improve RNA yield. Our method improves the utility of FFPE tissue for molecular profiling studies...
  58. ncbi A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues
    Teresa Rodríguez-Rigueiro
    Translational Cancer Research Group, Proteo Red ISCIII, Instituto de Investigación Biomédica A Coruña, Coruna, Spain
    Proteomics 11:2555-9. 2011
    ..This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases...
  59. ncbi Formaldehyde substitute fixatives: effects on nucleic acid preservation
    Cathy B Moelans
    Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Clin Pathol 64:960-7. 2011
    ..However, the commonly used formalin fixative is carcinogenic, and its crosslinking impairs DNA and RNA quality...
  60. ncbi Analysis of DNA methylation in FFPE tissues using the MethyLight technology
    Ashraf Dallol
    King Fahad Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
    Methods Mol Biol 724:191-204. 2011
    ..We describe in this chapter a detailed protocol for MethyLight and its use in the determination of CpG Island Methylator Phenotype status in FFPE colorectal cancer samples...
  61. ncbi Assessment of morphology, antigenicity, and nucleic acid integrity for diagnostic thyroid pathology using formalin substitute fixatives
    Sandra Lassalle
    Inserm ERI 21, Faculty of Medicine, University of Nice Sophia Antipolis, 06107 Nice, France
    Thyroid 19:1239-48. 2009
    ....
  62. ncbi Effects of preanalytical variables on the detection of proteins by immunohistochemistry in formalin-fixed, paraffin-embedded tissue
    Kelly B Engel
    Preferred Staffing Group for Office of Biorepositories and Biospecimen Research, National Cancer Institute, Washington, DC, USA
    Arch Pathol Lab Med 135:537-43. 2011
    ....
  63. ncbi Competitive allele specific TaqMan PCR for KRAS, BRAF and EGFR mutation detection in clinical formalin fixed paraffin embedded samples
    Audrey Didelot
    UMR S775, INSERM, Paris, F 75006, France
    Exp Mol Pathol 92:275-80. 2012
    ..The development of targeted therapies has created a need for robust molecular characterization of cancer and it has become a challenge to validate methods to ensure accuracy in tumor mutation testing...
  64. pmc The effects of brain tissue decomposition on diffusion tensor imaging and tractography
    Helen D'Arceuil
    Athinoula Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, MGH NMR Center, Rm 2301, Bldg 149, 13th St, Charlestown, MA 02129, USA
    Neuroimage 36:64-8. 2007
    ..postmortem, this is not possible for human tissue, therefore there is always some delay between death and tissue fixation. The elapsed time between death and tissue fixation, the postmortem interval (PMI), will most likely ..
  65. pmc Comparison of histopathology to gene expression profiling for the diagnosis of metastatic cancer
    Anand Kulkarni
    University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Diagn Pathol 7:110. 2012
    ..Gene expression profiling (GEP) has found increasing use, particularly in the most difficult cases. In this pilot study, a direct comparison between GEP and IHC-guided methods was performed...
  66. pmc Dramatic reduction of sequence artefacts from DNA isolated from formalin-fixed cancer biopsies by treatment with uracil- DNA glycosylase
    Hongdo Do
    Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia
    Oncotarget 3:546-58. 2012
    ....
  67. ncbi Comparison of formalin-free tissue fixatives: a proteomic study testing their application for routine pathology and research
    Hannelore Kothmaier
    Institute of Pathology, Research Unit for Molecular Lung and Pleura Pathology, Medical University of Graz, Austria
    Arch Pathol Lab Med 135:744-52. 2011
    ..Formalin-fixed, paraffin-embedded tissue is the routine processing method for diagnostics practiced in pathology departments worldwide...
  68. ncbi Ultrasound-accelerated formalin fixation improves the preservation of nucleic acids extraction in histological sections
    Tony Fracasso
    Institute of Legal Medicine, University of Munster, Röntgenstrasse 23, 48149 Munster, Germany
    Int J Legal Med 123:521-5. 2009
    ..Keeping in mind the increasing need for molecular diagnosis, this fixation technique can be useful to ensure stable quality of nucleic acids in archived autopsy specimens...
  69. pmc Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM
    Lasse S Kristensen
    Institute of Human Genetics, University of Aarhus, The Bartholin Building, Aarhus C, Denmark
    BMC Cancer 9:453. 2009
    ..Sensitive Melting Analysis after Real Time - Methylation Specific PCR (SMART-MSP) and Methylation Sensitive - High Resolution Melting (MS-HRM) are two methods for single locus DNA methylation detection based on HRM...
  70. ncbi The effects of tissue fixation alternatives on DNA content: a study on normal colon tissue
    Gulcan Baloglu
    Department of Biochemistry, Anatolian Health Center, Izmit, Turkey
    Appl Immunohistochem Mol Morphol 16:485-92. 2008
    ..All tissues fixed in formalin, ethanol, and Hollande were found suitable for Her-2/neu visualization after standard FISH applications, but tissues fixed in Zenker, B5, and Bouin were not found suitable...
  71. ncbi Advanced laboratory techniques for sample processing and immunolabeling using microwave radiation
    Adam M Ferris
    Department of Biological Sciences, California State University, Chico, CA 95929 0515, USA
    J Neurosci Methods 182:157-64. 2009
    ..Microwave-assisted immunohistochemistry made it possible to sequentially label tissues and cells with several primary antibodies in a very short period of time with excellent labeling characteristics...
  72. pmc Analytical performance of a PCR assay for the detection of KRAS mutations (codons 12/13 and 61) in formalin-fixed paraffin-embedded tissue samples of colorectal carcinoma
    Sung Lee
    Roche Molecular Systems, Inc, 4300 Hacienda Drive, Pleasanton, CA 94588, USA
    Virchows Arch 460:141-9. 2012
    ..The PCR assay was more sensitive and specific than Sanger sequencing, and performance was highly reproducible. Test performance was not influenced by various endogenous interfering substances or common gut microbes...
  73. ncbi Effects of temperature and aldehyde fixation on tissue water diffusion properties, studied in an erythrocyte ghost tissue model
    Peter E Thelwall
    Department of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, Florida, USA
    Magn Reson Med 56:282-9. 2006
    ..Water membrane permeability was also significantly altered by fixation. This study demonstrates that relating in vivo MR data to chemically fixed ex vivo data requires an understanding of the effects of sample preparation...
  74. ncbi Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges
    Niroshini J Nirmalan
    Clinical and Biomedical Proteomics Group, Cancer Research UK Clinical Centre, St James s University Hospital, Beckett Street, Leeds, UK
    Mol Biosyst 4:712-20. 2008
    ..Here, we critically review the current status of research in the field, highlighting challenges which need to be addressed for robust quantitative application of protocols to ensure confident high impact inferences can be made...
  75. ncbi Whole genome amplification of DNA extracted from FFPE tissues
    Mira Bosso
    Faculty of Medicine, Department of Pathology, Kuwait University, Safat, Kuwait
    Methods Mol Biol 724:161-80. 2011
    ..GenomePlex(®) is a registered trademark of Rubicon Genomics Incorporation...
  76. pmc Automated array-CGH optimized for archival formalin-fixed, paraffin-embedded tumor material
    Simon A Joosse
    Division of Experimental Therapy, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    BMC Cancer 7:43. 2007
    ..Recently, we have developed an effective DNA quality test that predicts suitability of archival samples for BAC aCGH...
  77. ncbi Genome-wide, high-resolution detection of copy number, loss of heterozygosity, and genotypes from formalin-fixed, paraffin-embedded tumor tissue using microarrays
    Sharoni Jacobs
    Affymetrix, Inc, Santa Clara, California 95051, USA
    Cancer Res 67:2544-51. 2007
    ....
  78. pmc Whole genome amplification for array comparative genomic hybridization using DNA extracted from formalin-fixed, paraffin-embedded histological sections
    Jian Huang
    International Agency for Research on Cancer, Lyon, France
    J Mol Diagn 11:109-16. 2009
    ..0001). These results suggest that genetic analyses are possible using WGA-DNA extracted from paraffin sections, but that they should be performed with a carefully optimized and controlled protocol...
  79. ncbi The HOPE technique opens up a multitude of new possibilities in pathology
    E Vollmer
    Clinical and Experimental Pathology, Research Center Borstel, Germany
    Rom J Morphol Embryol 47:15-9. 2006
    ..Therefore new possibilities are opened up especially within the rapidly growing field of molecular pathology...
  80. ncbi RNA extraction from archival formalin-fixed paraffin-embedded tissue: a comparison of manual, semiautomated, and fully automated purification methods
    Kerstin Bohmann
    Siemens Healthcare Diagnostics Products, Molecular Research Germany, Cologne, Germany
    Clin Chem 55:1719-27. 2009
    ..A robust and automated RNA-extraction method with a high sample throughput is required...
  81. pmc Development of a microarray platform for FFPET profiling: application to the classification of human tumors
    Sven Duenwald
    Translational Sciences, Department of Molecular Profiling, Merck Research Laboratories, Seattle, WA 98109, USA
    J Transl Med 7:65. 2009
    ..Methods are needed to profile these FFPET samples that are linked to clinical outcomes to generate hypotheses that could lead to classifiers for clinical applications...
  82. pmc Simultaneous recovery of DNA and RNA from formalin-fixed paraffin-embedded tissue and application in epidemiologic studies
    Wen Yi Huang
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Boulevard, EPS 8110, MSC 7240, Bethesda, MD 20892 7240, USA
    Cancer Epidemiol Biomarkers Prev 19:973-7. 2010
    ..Here, we report the method that we used to simultaneously recover DNA and RNA from FFPE tissue specimens with appreciable quantity and quality and discuss briefly the application of tumor markers in epidemiologic studies...
  83. ncbi Excavation of a buried treasure--DNA, mRNA, miRNA and protein analysis in formalin fixed, paraffin embedded tissues
    R Klopfleisch
    Department of Veterinary Pathology, Freie Universitat Berlin, Robert von Ostertag Straße 15, Berlin, Germany
    Histol Histopathol 26:797-810. 2011
    ..In addition, methods and general problems of DNA, miRNA, mRNA and protein extraction and the current knowledge on the feasibility and accuracy of quantitative gene expression analysis in FFPE tissues is summarized...
  84. pmc Improving molecular detection of fungal DNA in formalin-fixed paraffin-embedded tissues: comparison of five tissue DNA extraction methods using panfungal PCR
    C Muñoz-Cadavid
    Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 48:2147-53. 2010
    ..Factors such as sensitivity, cost, and labor will help guide the selection of the most appropriate method for the needs of each laboratory...
  85. ncbi Effect of duration of fixation on quantitative reverse transcription polymerase chain reaction analyses
    Maricris Macabeo-Ong
    Oral Pathology, Department of Stomatology, University of California San Francisco, California 94143 0424, USA
    Mod Pathol 15:979-87. 2002
    ..However, results for some markers are adversely affected by prolonged formalin fixation times...
  86. ncbi Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting
    Niroshini J Nirmalan
    Clinical and Biomedical Proteomics Group, Cancer Research UK Clinical Centre, Leeds, UK
    J Pathol 217:497-506. 2009
    ....
  87. pmc Successful protein extraction from over-fixed and long-term stored formalin-fixed tissues
    Claudia Wolff
    Institute of Pathology, Technische Universitat Munchen, Munich, Germany
    PLoS ONE 6:e16353. 2011
    ..Because tissue fixation times vary in the routine clinical setting and pathology archives contain billions of FFPE tissues blocks, ..
  88. pmc Profiling and discovery of novel miRNAs from formalin-fixed, paraffin-embedded melanoma and nodal specimens
    Zhihai Ma
    Departments of Pathology, Stanford University School of Medicine, Stanford, California, USA
    J Mol Diagn 11:420-9. 2009
    ..This methodology can therefore be used to facilitate miRNA discovery from archived human cancers...
  89. ncbi Tissue preparation: Tissue issues
    Nathan Blow
    Nature 448:959-63. 2007
  90. ncbi Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM)
    W Baschong
    M E Muller Institute at the Biozentrum, Aarau, Switzerland
    J Histochem Cytochem 49:1565-72. 2001
    ..Sudan Black B is suggested to mask fluorescent tissue components...
  91. ncbi Genome-wide DNA methylation analysis of archival formalin-fixed paraffin-embedded tissue using the Illumina Infinium HumanMethylation27 BeadChip
    Christina Thirlwell
    Medical Genomics Group, UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, UK
    Methods 52:248-54. 2010
    ..Here, we describe a protocol for the analysis of FFPE samples using the Infinium HumanMethylation27 BeadChip...
  92. ncbi MicroRNA isolation from formalin-fixed, paraffin-embedded tissues
    Aihua Liu
    University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Methods Mol Biol 724:259-67. 2011
    ..We demonstrate that miRNA expression profile from FFPE tissues closely resembles that from fresh tissues. These results underscore the suitability of FFPE tissues as appropriate resources for miRNA expression analyses...
  93. pmc Exon-array profiling unlocks clinically and biologically relevant gene signatures from formalin-fixed paraffin-embedded tumour samples
    J S Hall
    Translational Radiobiology Group, School of Cancer and enabling Sciences, Manchester Academic Health Science Centre, The University of Manchester, Wilmslow Road, Manchester M20 4BX, UK
    Br J Cancer 104:971-81. 2011
    ..This study aimed to show that useful information can be obtained by Exon-array profiling archival FFPE tumour samples...
  94. pmc Genome-wide massively parallel sequencing of formaldehyde fixed-paraffin embedded (FFPE) tumor tissues for copy-number- and mutation-analysis
    Michal R Schweiger
    Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
    PLoS ONE 4:e5548. 2009
    ..However, FFPE preparation is incompatible with many down-stream molecular biology techniques such as PCR based amplification methods and gene expression studies...
  95. pmc Modeling formalin fixation and antigen retrieval with bovine pancreatic RNase A II. Interrelationship of cross-linking, immunoreactivity, and heat treatment
    Vladimir K Rait
    Division of Biophysics, Department of Cellular Pathology and Genetics, Armed Forces Institute of Pathology, Rockville, MD 20850, USA
    Lab Invest 84:300-6. 2004
    ..We conclude that formaldehyde cross-links stabilize antigens against the denaturing effects of high temperature, but the reversal of these cross-links is necessary for the restoration of immunoreactivity...
  96. ncbi An approach to high resolution diffusion tensor imaging in fixed primate brain
    Susan Westmoreland
    Neuroradiology Section, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, USA
    Neuroimage 35:553-65. 2007
    ....
  97. ncbi A molecular mechanism of formalin fixation and antigen retrieval
    Seshi R Sompuram
    Department of Pathology and Laboratory Medicine, Boston University School of Medicine, MA, USA
    Am J Clin Pathol 121:190-9. 2004
    ....
  98. ncbi Liquid Tissue: proteomic profiling of formalin-fixed tissues
    Darue A Prieto
    SAIC Frederick, Inc, National Cancer Institute, Frederick, MD, USA
    Biotechniques . 2005
    ....
  99. ncbi Unlocking pathology archives for microRNA-profiling
    Kai P Hoefig
    Institute for Pathology, UKSH Campus Luebeck, Luebeck, Germany
    Anticancer Res 28:119-23. 2008
    ..A variety of high-throughput screening methods has been developed to identify dysregulated miRNA species in tumours. For retrospective clinical studies formalin-fixed, paraffin-embedded (FFPE) tissue is the most widely used material...
  100. ncbi Extraction and amplification of DNA from formalin-fixed, paraffin-embedded tissues
    Lin Wu
    Department of Human Genetics, Roche Molecular Systems, Inc, Alameda, California 94501, USA
    Appl Immunohistochem Mol Morphol 10:269-74. 2002
    ..We also have obtained much higher amplification efficiencies for a multiplex polymerase chain reaction...
  101. ncbi Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids
    Matthias Preusser
    Department of Medicine I, Medical University of Vienna, Vienna, Austria
    Brain Pathol 20:1010-20. 2010
    ..In conclusion, RCL2 fixation of brain tumor biopsies does not seem to significantly compromise histological tumor typing or immunohistochemistry and preserves nucleic acids (DNA and RNA) at a better quality than formalin fixation...

Research Grants62

  1. New Methods for Tissue Fixation
    LOUIS ROMANO; Fiscal Year: 2003
    ..abstract_text> ..
  2. Cortical Synapses and Psychosis in AD
    Robert A Sweet; Fiscal Year: 2012
    ..The laboratories consist of separate facilities for tissue fixation and preparation, immunocytochemistry and other histological procedures, western blotting, and computer-..
  3. Methods and Instrumentation for Hydrostatic Pressure-Enhanced Tissue Fixation
    Alexander V Lazarev; Fiscal Year: 2011
    ..an automated tissue preservation system that uses high hydrostatic pressure to standardize the process of tissue fixation with precise thermodynamic control...
  4. Mid-Region AIDS and Cancer Specimen Resource Consortium
    Leona W Ayers; Fiscal Year: 2012
    ..MR-ACSR proposes the deployment of a new tissue fixation method to improve preservation of RNA, DNA and tissue proteins for research tissues...
  5. Transgenic Reporters for Cardiac Growth and Regeneration
    Loren J Field; Fiscal Year: 2010
    ..over existing models in that data can be acquired with minimal sample processing (in essence, requiring only tissue fixation and sectioning)...
  6. Develop & Validate An Ultrasound Tissue Preservation De*
    Zhenqing Zhu; Fiscal Year: 2006
    ..Our proposed project is to design and develop an ultrasound-facilitated processor (DTP) for rapid tissue fixation and processing for histology diagnosis and any further molecular study if necessary...
  7. Molecular profiles of colorectal adenomas by array CGH
    Galen Hostetter; Fiscal Year: 2009
    ..b>Tissue fixation: 10% buffered formalin. Microarray: Agilent 60-mer oligonucleotide microarrays and image scanner...
  8. Tissue Lysates for Studies of Protein Phosphorylation
    Beatrice Knudsen; Fiscal Year: 2009
    ..grant is to identify optimal conditions for preparation of tissue samples by testing multiple combinations of tissue fixation and protein extraction buffers...
  9. High Impedance Pacing Electrode with Novel Tine Fixation
    ALAN GRESZLER; Fiscal Year: 2004
    ..the much greater mechanical strain on these leads, the fixation mechanism must be very secure until chronic tissue fixation, due to tissue in-growth, is complete...
  10. Enabling RNA Analyses from Formaldehyde-fixed Tissues
    Richard Conrad; Fiscal Year: 2006
    ..The widespread use of tissue fixation and embedding in both clinical and research settings has resulted in the accumulation of large stores of ..
  11. Synthetic calibrators for immunohistochemical assays
    STEVEN BOGEN; Fiscal Year: 2002
    ..A second set of processing calibrators will control for pre-analytic variability, which is associated with tissue fixation or antigen retrieval...
  12. MODULATION OF AIRWAY INFLAMMATION IN ASTHMA MODELS
    David Center; Fiscal Year: 2005
    ..A. Marshak-Rothstein which provides a central facility for breeding all animals, immunization and challenge for physiology and tissue fixation for evaluation of inflammation.
  13. Fine Needle Aspiration Biopsy(FNAB) Needle Device
    Vinod Shidham; Fiscal Year: 2002
    ..Apart from the worldwide market, the potential market in United States alone should exceed $1,500,000 per year. ..
  14. Ultrasound-accelerated tissue formalin fixation and paraffin embedding
    Wei Sing Chu; Fiscal Year: 2008
    ..a multi- tissue preservation processor with an optional real-time digital system to monitor and standardize tissue fixation level; (2)to validate the UTP techniques by performing more statistical assessments on histopathology, ..
  15. Soft Tissue Biomechanical Behavior During Acupuncture in Low Back Pain
    Helene M Langevin; Fiscal Year: 2010
    ....
  16. BIOMECHANICAL EFFECT OF ACUPUNCTURE NEEDLING
    HELENE LANGEVIN; Fiscal Year: 2001
    ..This will contribute to the fundamental understanding of acupuncture therapy and may lead to improvements in treatments. ..
  17. THE EFFECT OF LYCOPENE ON IGF-1 ACTIVITY AND SECRETION IN PRIMARY CULTURES OF PRO
    Larisa Nonn; Fiscal Year: 2008
    ..Once we have determined lycopene's effect on the IGF-1-induced actions in normal prostatic cells, this effect will be compared to those on cells derived from adenocarcinoma. [unreadable] [unreadable] [unreadable]..
  18. Mechanisms of Radiation-Induced Cognitive Dysfunction
    William Brown; Fiscal Year: 2008
    ..Such therapeutic intervention studies could potentially be performed in as little as 10 weeks. ..
  19. VAS-COG 2007
    Donald Royall; Fiscal Year: 2007
    ..Many vascular cognitive disorders are preventable and potentially reversible. VAS-COG will provide a forum in which information on these important conditions can be exchanged and disseminated. [unreadable] [unreadable] [unreadable]..
  20. IKSI: 1st Annual International Urolithiasis Research Symposium
    Andrew Evan; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  21. Nicotine and Cognition in Parkinsonism
    Jay Schneider; Fiscal Year: 2008
    ..Information obtained from these studies will hopefully lead to more efficient design of nicotinic therapeutics for patients with a variety of cognitive disorders. ..
  22. Mechanisms Causing Cryptorchidism and Fetal Transformation of Testicular Cells
    D N Rao Veeramachaneni; Fiscal Year: 2007
    ..Results will clarify the interaction of genetics and EDAs as cause of cryptorchidism. [unreadable] [unreadable] [unreadable]..
  23. HAIR CELL RESPONSES TO OTOTOXIC DRUGS
    PETER STEYGER; Fiscal Year: 2007
    ..This may lead to new co-therapeutic treatments with aminoglycosides and (cellular) aminoglycoside-uptake inhibitors against a wider range of bacterial infections. ..
  24. MINERALIZATION OF PRIMARY BONE
    JEFFREY GORSKI; Fiscal Year: 2008
    ..Results of this work should better define the differences between primariy and lamellar bone, as well as aid in the diagnosis and treatment of osteoporosis and atherosclerosis. ..
  25. Assessing Deployment of Microbicidal Gels With Label-Free Optical Measurement
    Adam Wax; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  26. Jak/STAT signaling in human T-cell lymphomas
    Mariusz A Wasik; Fiscal Year: 2010
    ....
  27. The Cardiac Atlas Project: Establishment of a Cardiac MRI Database.
    ALISTAIR ANDREW YOUNG; Fiscal Year: 2010
    ..By pooling data from many studies into a single resource, researchers will be able to perform standardized comparisons across many patient groups, in order to better predict and evaluate the effects of disease and treatment. ..
  28. Simvastatin treatment of experimental intracerebral hemorrhage
    DONALD M SEYFRIED; Fiscal Year: 2010
    ....
  29. Coherence Imaging for Assessing Colorectal Neoplasia
    Adam Wax; Fiscal Year: 2010
    ..abstract_text> ..
  30. Nongenomic Signaling Mechanisms of Environmental Estrogens
    Cheryl S Watson; Fiscal Year: 2011
    ..breast and pituitary cancers, infertility) must be understood so that exposures can be limited to safe levels. ..
  31. Spinal cord repair with nerve constructs
    Douglas Smith; Fiscal Year: 2009
    ..abstract_text> ..
  32. Organization and Regulation of Eukaryotic Centromeres
    BETH SULLIVAN; Fiscal Year: 2009
    ..We propose to investigate the roles of genomic structure and chromatin organization and dynamics in assembly and regulation of CENP-A chromatin, centromeric domains, and metaphase kinetochore structure. ..
  33. CHAOTIC MIXING OF AEROSOL IN RHYTHMICALLY EXPANDING LUNG
    Akira Tsuda; Fiscal Year: 2008
    ..abstract_text> ..
  34. Blood Brain Barrier Physiology and HIV Dementia
    HOWARD GENDELMAN; Fiscal Year: 2007
    ..The data acquired could provide new insights and therapeutic intervention strategies for monocyte BBB trafficking during progressive HIV-1 infection in brain tissue. ..
  35. Replacing an Animal Resource Facility Cage Washer.
    Kristen Drescher; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  36. GM1 Ganglioside Effects on Parkinson's Disease
    Jay Schneider; Fiscal Year: 2007
    ..Working hypothesis: Long-term GM1 use will stabilize symptoms or slow the progression of symptoms in PD patients and this may be accompanied by reduced loss of striatal DA terminals over time. [unreadable] [unreadable]..
  37. MURINE MODEL FOR MONKEY B VIRUS INFECTION
    JERRY RITCHEY; Fiscal Year: 2004
    ..abstract_text> ..
  38. BRAIN INJURY, AGING AND NEURODEGENERATION IN THE RAT
    Douglas Smith; Fiscal Year: 2004
    ..Success of these studies may reveal mechanistic links between brain trauma, age and neurodegenerative processes and provide a basis for the development of new therapeutic strategies. ..
  39. Functional similarities in renal and cochlear epithelia
    PETER STEYGER; Fiscal Year: 2004
    ....
  40. ANALYSIS OF STRIATAL SPECIFIC RGS9
    Michael Bannon; Fiscal Year: 2004
    ..Determine the cellular localization and dopaminergic regulation of RGS9 in the striatum. Specific Aim 2. Identify and analyze RGS signaling partners. Specific Aim 3. Analyze RGS9 targeting and function. ..
  41. Environmental Estrogens Acting via a Membrane Receptor
    CHERYL WATSON; Fiscal Year: 2004
    ..abstract_text> ..
  42. A 3D C57BL6/J MOUSE BRAIN ATLAS MAGNETIC RESONANCE MICRO
    Helene Benveniste; Fiscal Year: 2003
    ..Further, a probabilistic (averaged from many brain specimens) atlas with error estimations will be created for registration of actual gene-expression data. ..
  43. Plasticity in the dorsal cochlear nucleus
    GULAMALI EMADI; Fiscal Year: 2003
    ..abstract_text> ..
  44. A Method To Study Membrane Protein Structure
    Guido Zampighi; Fiscal Year: 2003
    ....
  45. TEM with Digital and Cryopreparation Systems
    Andrew Evan; Fiscal Year: 2003
    ..Dr. Richard Gregory will employ immunohistochemistry to examine the role of a Streptococcus mutans 65 kDa fimbrial protein binding as a mechanism for the induction of dental carries. ..
  46. AMYLOIDOGENIC EFFECTS OF CHOLINERGIC DENERVATION
    Thomas Beach; Fiscal Year: 2002
    ..This could lead to impaired cerebral vasoregulation and increased susceptibility to ischemia, further compromising cortical function in AD. ..
  47. A HIGH PRESSURE FREEZER FOR ELECTRON MICROSCOPY USES
    GINA SOSINSKY; Fiscal Year: 2002
    ..In all of these research projects, the primary goal is to understand the three-dimensional structure of organized cellular components and relate this morphology to their functionality...
  48. REGULATION OF EXTRACELLULAR SUPEROXIDE DISMUTASE BY PROT
    Russell Bowler; Fiscal Year: 2004
    ..This will not only define new aspects of the pathogenesis and regulation of vascular tone and inflammation, but can open new approaches to pharmacologically control these events. ..
  49. GENE-EXPRESSION CHANGE IN HUMAN BLOOD SAMPLES AND ANTI-DEPRESSANT RESPONSE
    SAMUEL SATHYANESAN; Fiscal Year: 2004
    ..Briefly, further studies would involve examening differences among different types of depressive disorders and the correlation between gene-expression changes and treatment-response. ..
  50. THE ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
    Michael Freemark; Fiscal Year: 2007
    ..The results of our studies should provide new insist into the roles of pituitary and placental hormones in adipose development and function. ..
  51. Effect of GnRH antagonist on primordial follicle survival in the primate ovary
    DOUGLAS DANFORTH; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  52. Auditory cortex asymmetry in normals and schizophrenics
    John Smiley; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  53. Rediscovering B cells: a Trudeau Institute workshop
    Troy Randall; Fiscal Year: 2006
    ..unreadable] This proposal requests funds to support the attendance of five of these early-career investigators. ..
  54. Harbor-UCLA Initiative for Minority Student Development
    Christina Wang; Fiscal Year: 2005
    ....
  55. Determinants of the Durability of Resin-Dentin Bonds
    David Pashley; Fiscal Year: 2006
    ..This should provide important new insight into designing more durable resin-dentin bonds that can justify the placement of resin-based posterior composites. ..
  56. HIV 1 SUSCEPTIBILITY FACTORS IN THE FEMALE GENITAL TRACT
    Bruce Patterson; Fiscal Year: 2003
    ..Hypothesis confirmation will constitute a rationale for a clinical trial of these or similar agents applied topically, in vivo, to the female genital tract. ..
  57. Cloning Kawasaki Disease-specific antigens
    Anne Rowley; Fiscal Year: 2005
    ..These plans will provide me with opportunities for enhanced career development by acquisition of new research skills, and will be fostered by the strong interdisciplinary research environment at Northwestern. ..
  58. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    LAURA NGWENYA; Fiscal Year: 2005
    ..It has been established that cognitive decline occurs with age. This proposed investigation will determine if there are changes in adult cell genesis that could be important in the neurobiological basis of age-related cognitive decline. ..
  59. ALCOHOL, HIV, AND THE BRAIN
    Adolf Pfefferbaum; Fiscal Year: 2004
    ..Specific Aim 3: To establish cross-sectional and longitudinal within-subject relationships among nueroimaging, neuropsychological, and clinical measures. ..
  60. Prostate Cancer Development & Growth
    Christina Wang; Fiscal Year: 2004
    ..The results will provide the important missing link between diet fat and CaP growth and development. ..
  61. IMAGING TRANSMISSION ELECTRON MICROSCOPE
    VIRGINIA PICKEL; Fiscal Year: 2001
    ..abstract_text> ..