Summary: A modification of the freeze-drying method in which the ice within the frozen tissue is replaced by alcohol or other solvent at a very low temperature.
Publications209 found, 100 shown here
- A rapid microbiopsy system to improve the preservation of biological samples prior to high-pressure freezingD Vanhecke
Institute of Anatomy, University of Bern, Bern, Switzerland
J Microsc 212:3-12. 2003..We show that a range of animal tissues (liver, brain, kidney and muscle) are well preserved. To prove the quality of freezing achieved with the system, we show vitrified ivy leaves high-pressure frozen in the new specimen platelet...
- Direct attachment of cell suspensions to high-pressure freezing specimen planchettesA Sawaguchi
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
J Microsc 212:13-20. 2003..The aluminium planchettes appear to have little effect on cell physiology, as demonstrated by the fact that parietal cells cultured for 24-28 h on the planchettes retain their responsiveness to stimulation with histamine...
- The use of filter membranes for high-pressure freezing of cell monolayersM K Morphew
The Boulder Laboratory for 3 D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
J Microsc 212:21-5. 2003..Our protocol results in good preservation of cellular ultrastructure, it is a useful companion to studies of cell physioloy and, with some limitation, is suitable for correlative LM and EM...
- Epoxy resin as fixative during freeze-substitutionNadezda Matsko
Electron Microscopy Center, Institute of Applied Physics, ETH Hoenggerberg, CH 8093, Zuerich, Switzerland
J Struct Biol 152:92-103. 2005..The block face of epoxy stabilized samples after ultrathin sectioning is highly suited for the analysis of the ultrastructure by AFM...
- Freeze-substitution: the addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60 degrees CC Buser
Zentrale Einrichtung Elektronenmikroskopie, Universitaet Ulm, Albert Einstein Allee 11, 89069 Ulm, Germany
J Microsc 230:268-77. 2008High-pressure freezing followed by freeze substitution and plastic embedding is becoming a more widely used method for TEM sample preparation...
- Freeze substitution in 3 hours or lessK L McDonald
Electron Microscope Laboratory, University of California, Berkeley, CA 94720, USA
J Microsc 243:227-33. 2011b>Freeze substitution is a process for low temperature dehydration and fixation of rapidly frozen cells that usually takes days to complete...
- Silver enhancement of Nanogold particles during freeze substitution for electron microscopyM Morphew
Laboratory for 3D Electron Microscopy of Cells, Department of M C D Biology, University of Colorado, Boulder, CO 80309 0347, USA
J Microsc 230:263-7. 2008Recent advances in rapid freezing and fixation by freeze substitution have allowed structural cell biologists to apply these reliable modes of sample preparation to a wide range of specimens and scientific problems...
- Freeze substitution followed by low melting point wax embedding preserves histomorphology and allows protein and mRNA localization techniquesIván Durán
Department of Cell Biology, Genetics and Physiology, University of Malaga, Faculty of Sciences, Campus de Teatinos, Malaga 29071, Spain
Microsc Res Tech 74:440-8. 2011..This method, which we have named CryoWax, involves freeze substitution of the samples in isopentane and methanol, followed by embedding in low melting point polyester wax...
- Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains waterP Walther
Zentrale Einrichtung Elektronenmikroskopie, Universitaet Ulm, Albert Einstein Allee 11, 89069 Ulm, Germany
J Microsc 208:3-10. 2002..The effect was especially pronounced in yeast cells, where membrane visibility was poor after freeze-substitution with water-free medium but good after addition of 5% water to the substitution medium...
- Imaging the endothelial glycocalyx in vitro by rapid freezing/freeze substitution transmission electron microscopyEno E Ebong
Department of Biomedical Engineering, City College of New York, NY, USA
Arterioscler Thromb Vasc Biol 31:1908-15. 2011..We measured in vitro GCX thickness using rapid freezing/freeze substitution (RF/FS) transmission electron microscopy (TEM), taking advantage of the high spatial resolution provided by ..
- Immunocytochemical demonstration of the secretory dynamics of zymogenic contents in rat gastric gland processed by high-pressure freezing/freeze substitution, with special references to phospholipase A(2) and phospholipase Cgamma1A Sawaguchi
Department of Anatomy, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889-1692, Japan
Histochem Cell Biol 116:361-9. 2001High-pressure freezing/freeze substitution followed by Lowicryl K4M embedding provided an excellent morphology and antigenicity of the gastric glands, as well as the intraluminal fluid contents...
- High pressure freezing and freeze substitution of Schizosaccharomyces pombe and Saccharomyces cerevisiae for TEMStephen Murray
TEM Service Facility, Paterson Institute for Cancer Research, University of Manchester, Manchester, United Kingdom
Methods Cell Biol 88:3-17. 2008..The use of low temperature techniques and in particular high pressure freezing (HPF) and freeze substitution (FS) overcomes many of these problems...
- A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze substitutionB F McEwen
Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, P O Box 509, Albany, NY 12201 0509, USA
Chromosoma 107:366-75. 1998..somatic (PtK1) cells prepared for optimal structural preservation using high-pressure freezing and freeze substitution. In serial thin sections, and electron tomographic reconstructions, the kinetochore appears as a 50-75 nm ..
- High-pressure freezing and freeze substitution of rat myocardium for immunogold labeling of connexin 43Christian Mühlfeld
Division of Electron Microscopy, Department of Anatomy, University of Gottingen, Gottingen, Germany
Anat Rec A Discov Mol Cell Evol Biol 288:1059-67. 2006The value of high-pressure freezing (HPF) and freeze substitution (FS) for immunoelectron microscopy (immuno-EM) of the heart was investigated in bioptic specimens taken from isolated hearts of 0-, 5-, and 14-day-old rats at baseline and ..
- Electron tomographic analysis of somatic cell plate formation in meristematic cells of Arabidopsis preserved by high-pressure freezingJose M Segui-Simarro
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309 0347, USA
Plant Cell 16:836-56. 2004..ER progressively accumulates thereafter, reaching a maximum during the late PFS stage, when most cell plate growth is completed...
- High-pressure freezing in the study of animal pathogensP Monaghan
Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
J Microsc 212:62-70. 2003....
- Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructureT Müller-Reichert
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstr 108, D 01307 Dresden, Germany
J Microsc 212:71-80. 2003..The latter method facilitates embedding of C. elegans in a thin layer of resin so individual worms can be staged, selected and precisely orientated for serial sectioning followed by immunolabelling or electron tomography...
- From tissue to cellular ultrastructure: closing the gap between micro- and nanostructural imagingS S Biel
Analytical Microscopy, Beiersdorf AG, Unnastrasse 48, D 20245 Hamburg, Germany
J Microsc 212:91-9. 2003..Then, the cell layer can be cut into a series of ultrathin sections and examined by EM for determination of the subcellular and nanostructural organization...
- Untangling desmosomal knots with electron tomographyWanzhong He
Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Science 302:109-13. 2003..This flexibility suggests a novel mechanism for generating both cis and trans interactions and for propagating these adhesive interactions along the junction...
- A morphological and immunolabeling study of freeze-substituted Bacteroides forsythusK Moriguchi
Department of Anatomy, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464 8650, Japan
Biotech Histochem 78:129-33. 2003..Our results show the usefulness of the freeze-substitution method for immunohistochemical studies of B. forsythus...
- Improved method for visualizing coated pits, microfilaments, and microtubules in cryofixed and freeze-substituted plant cellsTakashi Murata
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Japan
J Electron Microsc (Tokyo) 51:133-6. 2002..The combination of a 40 degrees C OsO4 staining step followed by staining with uranyl acetate at 4 degrees C should prove useful for more detailed plant cytoskeletal/membrane studies in the future...
- AA protein in experimental murine AA amyloid fibrils: a high resolution ultrastructural and immunohistochemical study comparing aldehyde-fixed and cryofixed tissuesSadayuk Inoue
Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
Amyloid 9:115-25. 2002..In this study, we have re-examined the AA amyloid fibrils with advanced methods of cryofixation and freeze substitution which are known to retain ultrastructural detail as close as possible to the living state...
- A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cellsA Sawaguchi
Department of Molecular and Cell Biology, University of California, 241 LSA, Berkeley, CA 94720 3200, USA
J Microsc 208:158-66. 2002..The present findings provide a clue to vesicular membrane trafficking in cultured gastric parietal cells, and assure the utility of the new procedure for high-pressure freezing of primary culture cells...
- Cryofixation of epithelial cells grown on sapphire coverslips by impact freezingS Reipert
Institute of Biochemistry and Molecular Cell Biology, University of Vienna, Vienna Biocenter, 1030 Vienna, Austria
J Microsc 209:76-80. 2003..The structural preservation of Lowicryl HM20-embedded cells has been assessed as being free of damage by large ice crystals...
- Two approaches to double post-embedding immunogold labeling of freeze-substituted tissue embedded in low temperature Lowicryl HM20 resinS Mahendrasingam
MacKay Institute of Communication and Neuroscience, School of Life Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK
Brain Res Brain Res Protoc 11:134-41. 2003..This approach indicated that GAT1 occurs in the plasma membrane of some terminals that contain glycine. Thus, these techniques can be used to localize heat-labile multiple antigens in the same tissue...
- Comparative studies of the cell structures of Mycobacterium leprae and M. tuberculosis using the electron microscopy freeze-substitution techniqueAkemi Takade
Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Fukuoka 812 8582, Japan
Microbiol Immunol 47:265-70. 2003..The cell widths measured on electron micrographs were 0.44 microm for M. tuberculosis and 0.38 microm for M. leprae. The peptidoglycan layer of M. leprae was 4-5 nm, while the corresponding layer of M. tuberculosis was 10-15 nm...
- Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samplesT H Giddings
Department of Molecular, Cellular and Developmental Biology, Campus Box 347, University of Colorado, Boulder, CO 80309, USA
J Microsc 212:53-61. 2003..The tendency of permanganate to degrade cytoskeletal elements and other proteins when employed in aqueous solutions at room temperature is apparently curtailed when it is used as a freeze-substitution reagent...
- Quantitative three-dimensional structural analysis of Exophiala dermatitidis yeast cells by freeze-substitution and serial ultrathin sectioningSondip Kumar Biswas
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8673, Japan
J Electron Microsc (Tokyo) 52:133-43. 2003..This is the first report, to our knowledge, that analysed all the components in the yeast cell quantitatively and in three dimensions, and provides fundamental information for understanding various aspects of cell biology...
- Presynaptic membrane of inhibitory crayfish axon terminals is stained by antibodies raised against mammalian GABA(A) receptor subunits alpha3 and beta(2/3)N Feinstein
The Otto Loewi Minerva Center for Cellular and Molecular Neurobiology, The Hebrew University, Jerusalem 91904, Israel
J Comp Neurol 465:250-62. 2003..We suggest that presynaptic inhibitory boutons of the crayfish possess GABA(A)-like autoreceptors composed of at least the alpha3 and beta(2/3) subunits...
- Use of energy-filtering transmission electron microscopy for routine ultrastructural analysis of high-pressure-frozen or chemically fixed plant cellsU Lütz-Meindl
Institute of Plant Physiology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria
Protoplasma 223:155-62. 2004....
- Cryo-electron microscopy reveals native polymeric cell wall structure in Bacillus subtilis 168 and the existence of a periplasmic spaceValério R F Matias
Biophysics Interdepartmental Group and Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Mol Microbiol 56:240-51. 2005....
- Ultrastructural transformation of gastric parietal cells reverting from the active to the resting state of acid secretion revealed in isolated rat gastric mucosa model processed by high-pressure freezingAkira Sawaguchi
Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki, Miyazaki 889 1692, Japan
J Electron Microsc (Tokyo) 55:97-105. 2006..The present findings provide new insights into the functional transformation in gastric parietal cells reverting to their resting conformation...
- A new microbiopsy system enables rapid preparation of tissue for high-pressure freezingDimitri Vanhecke
Anatomical Institute, University of Bern, Switzerland
Methods Mol Biol 319:463-77. 2006..Thirty seconds preparation time is needed from excision until high-pressure freezing. Brain, liver, kidney and muscle excisions of anesthetised rats are shown to be well frozen...
- Cryo-electron microscopy of cell division in Staphylococcus aureus reveals a mid-zone between nascent cross wallsValério R F Matias
Department of Molecular and Cellular Biology College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Mol Microbiol 64:195-206. 2007..The two zones next to the membranes are periplasmic spaces. Immediately next to these are the growing cross walls composed of peptidoglycan, teichoic acid and protein...
- Rapid freeze-substitution preserves membranes in high-pressure frozen tissue culture cellsP Hawes
Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
J Microsc 226:182-9. 2007..2% resulted in improved labelling. Samples substituted in this lower concentration of uranyl acetate also gave good membrane detail when imaged after section contrasting...
- Distribution of immunoglobulin-producing cells in immunized mouse spleens revealed with "in vivo cryotechnique"Sei Saitoh
Department of Anatomy and Molecular Histology, University of Yamanashi, Chuo City, Yamanashi 409 3898, Japan
J Immunol Methods 331:114-26. 2008..The development of Ig-producing cells was clarified in the specimens prepared with IVCT, which proved to be useful for analyzing the native morphology and distribution of Ig-producing cells...
- Pseudovacuoles--immobilized by high-pressure freezing--are associated with blebbing in walker carcinosarcoma cellsD Vanhecke
Department for Topographic Anatomy and Neuroanatomy, Institute of Anatomy, University of Bern, Switzerland
J Microsc 230:253-62. 2008..After high-pressure freezing and freeze substitution, pseudovacuoles appeared to be filled with 20 nm large electron-transparent patches surrounded by 12 and 15 ..
- Controlled microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomographyW J Triffo
Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mail Stop Donner, Berkeley, CA 94720, USA
J Microsc 230:278-87. 2008..We illustrate the potential for correlative light and electron microscopy as well as electron tomography...
- Rapidly cooled human sperm: no evidence of intracellular ice formationG J Morris
Asymptote Ltd, St Johns Innovation Centre, Cambridge, UK
Hum Reprod 21:2075-83. 2006..Alternatively, the cell damage may be the result of an osmotic imbalance encountered during thawing. This article examines whether intracellular ice forms during rapid cooling or if an alternative mechanism is present...
- Aclar discs: a versatile substrate for routine high-pressure freezing of mammalian cell monolayersN Jimenez
Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
J Microsc 221:216-23. 2006..We present a complete protocol, which, because of its simplicity and reproducibility, provides a method suitable for the routine analysis of mammalian cell monolayers by electron microscopy and tomography...
- Visualization of the nuclear lamina in mouse anterior pituitary cells and immunocytochemical detection of lamin A/C by quick-freeze freeze-substitution electron microscopyTakao Senda
Department of Anatomy I, Fujita Health University School of Medicine, Toyoake, Aichi 470 1192, Japan
J Histochem Cytochem 53:497-507. 2005..lamina in the quickly frozen anterior pituitary cells by electron microscopic techniques combined with freeze substitution, deep etching, and immunocytochemistry and compared it with that in the chemically fixed cells...
- Use of surface affinity enrichment and cryo-embedding to prepare in vitro reconstituted mitotic chromosomes for EM tomographyPeter Konig
Department of Biochemistry and Biophysics, The Howard Hughes Medical Institute, University of California, San Francisco, CA 94143 2240, USA
Ultramicroscopy 103:261-74. 2005..The implications of the new method for the preparation of other difficult samples and additional application possibilities are discussed...
- Safe specimen preparation for electron microscopy of pathogenic fungi by freeze-substitution after glutaraldehyde fixationMasashi Yamaguchi
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan
Nihon Ishinkin Gakkai Zasshi 46:187-92. 2005..Although we have demonstrated the utility of this method using Exophiala dermatitidis and Cryptococcus neoformans, it could also be used for observing highly infectious fungi such as Coccidioides immitis...
- Organelle interactions and possible degradation pathways visualized in high-pressure frozen algal cellsN Aichinger
Plant Physiology, Cell Biology Department, University of Salzburg, Austria
J Microsc 219:86-94. 2005..Both the interactions between vesicles and organelles and the degradation pathways occur independently from cytoskeleton function as demonstrated by use of cytochalasin D and the microtubule inhibitor amiprophos-methyl...
- Chloroplast ultrastructure in leaves of Urtica dioica L. analyzed after high-pressure freezing and freeze-substitution and compared with conventional fixation followed by room temperature dehydrationStephan Pfeiffer
Central Microscopy, Center of Biology, University of Kiel, D 24098 Kiel, Germany
Microsc Res Tech 68:368-76. 2005....
- Application of in vivo cryotechnique to the examination of cells and tissues in living animal organsN Terada
Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Tamaho, Yamanashi, Japan
Histol Histopathol 21:265-72. 2006....
- Immunohistochemical detection of phosphorylated rhodopsin in light-exposed retina of living mouse with in vivo cryotechniqueNobuo Terada
Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Tamaho, Yamanashi 409 3898, Japan
J Histochem Cytochem 54:479-86. 2006..This is a new immunohistochemical approach to visualize the time-dependent Rho phosphorylation of living mice using the in vivo cryotechnique, in which changes could be detected within seconds following exposure to light...
- Preparation of cells and tissues for immuno EMPaul Webster
Cell Biology and Biophysics Unit, EMBL, Heidelberg, Meyerhofstrasse 69117, Germany
Methods Cell Biol 88:45-58. 2008..Given the space limitation, the fine details necessary to apply these methods have been successfully omitted and will have to be obtained from the technical references we provide...
- Ultrastructural localization of bcl-2 proteinP Monaghan
Department of Cell Biology and Experimental Pathology, Institute of Cancer Research, Sutton, Surrey, UK
J Histochem Cytochem 40:1819-25. 1992....
- Cryofixation, cryosubstitution, and immunoelectron microscopy: potential role in diagnostic pathologyJ A McCann
Cell Imaging Facility, University of Vermont, Burlington, USA
Ultrastruct Pathol 20:223-30. 1996..Good ultrastructural preservation was obtained and reasonable immunolabeling with antibodies to AE1/AE3 keratin filaments was also observed...
- Morphological study of erythrocyte shapes in red pulp of mouse spleens revealed by an in vivo cryotechniqueM Xue
Department of Anatomy, Yamanashi Medical University, Tamaho, Japan
Histol Histopathol 16:123-9. 2001..It is suggested that most erythrocytes congesting in spleens keep their original configuration in spite of microenviromental alteration in splenic blood circulation...
- X-ray microanalysis of biological specimens by high voltage electron microscopyTetsuji Nagata
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto 390 8621, Japan
Prog Histochem Cytochem 39:185-319. 2004..These methodologies should be utilized in microanalysis of various compounds and elements in various cells and tissues in various organs...
- Occurrence and ultrastructure of Albugo candida on a new host, Arabis alpina in Saudi ArabiaZ A M Baka
Department of Biology, College of Sciences, King Khalid University, Abha, P O Box 9004, Saudi Arabia
Micron 39:1138-44. 2008..Both conventional chemical fixation and high pressure freezing followed by freeze substitution (HPF/FS) were used to prepare zoosporangia, intercellular hyphae, haustoria, invading host cells and host-..
- The ultrastructure of the developing replication site in foot-and-mouth disease virus-infected BHK-38 cellsPaul Monaghan
Institute for Animal Health, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
J Gen Virol 85:933-46. 2004..With conventional fixation, FMDV particles were not seen; however, following high-pressure freezing and freeze-substitution, many clusters of virus-like particles were seen...
- Reevaluation of the effect of lysoyzme on Escherichia coli employing ultrarapid freezing followed by cryoelectronmicroscopy or freeze substitutionP Wild
Institute of Veterinary Anatomy, University of Zurich, Switzerland
Microsc Res Tech 39:297-304. 1997..coli, we employed cryotechniques including ultrarapid freezing, cryomicroscopy and freeze substitution, and immunolabeling...
- Cell cycle-dependent changes in Golgi stacks, vacuoles, clathrin-coated vesicles and multivesicular bodies in meristematic cells of Arabidopsis thaliana: a quantitative and spatial analysisJose M Segui-Simarro
Department of Molecular, Cellular and Developmental Biology, University of Colorado, UCB 347, Boulder, CO 80309 0347, USA
Planta 223:223-36. 2006....
- Temporary accumulation of glycogen in the epithelial cells of the developing mouse submandibular glandSachiko Matsuura
Department of Oral Histology, Matsumoto Dental University, Shiojiri, Japan
Anat Sci Int 82:164-74. 2007..in hydrophilic glycol methacrylate resin was used for light microscopy, and high-pressure freezing/freeze substitution for electron microscopy...
- Morphological studies on the translocation of tubulovesicular system toward the intracellular canaliculus during stimulation of the gastric parietal cellT Ogata
Department of Surgery, Kochi Medical School, Nankoku, Kochi, Japan
Microsc Res Tech 48:282-92. 2000..Special emphasis is made to demonstrate connections between the tubulovesicular system and the intracellular canaliculus...
- Low temperature and anhydrous electron microscopy techniques to observe the infection process of the bacterial pathogen Xanthomonas fragariae on strawberry leavesP Allan-Wojtas
Agriculture and Agri Food Canada, Atlantic Food and Horticulture Research Centre, Kentville, Nova Scotia, Canada B4N 1J5
J Microsc 239:249-58. 2010....
- Membrane vesicles: a common feature in the extracellular matter of cold-adapted antarctic bacteriaAlina Frias
Laboratori de Microbiologia, Facultat de Farmacia, Universitat de Barcelona, Av Joan XIII s n, 08028 Barcelona, Spain
Microb Ecol 59:476-86. 2010..and Marinobacter guineae M3B(T)) by transmission electron microscopy after high-pressure freezing and freeze substitution. All analyzed extracellular matter appeared as a netlike mesh composed of a capsular polymer around cells ..
- Envelope glycosylation determined by lectins in microscopy sections of Acinetobacter venetianus induced by diesel fuelFranco Baldi
Department of Environmental Sciences, Ca Foscari University, Calle Larga S Marta, Dorsoduro 2137, 30121 Venice, Italy
Res Microbiol 154:417-24. 2003..Samples were prepared by freeze substitution, which allows glycosylation to be determined in situ in thin sections of specimens...
- Structural evidence for counter-current flow in proximal tubules versus pertitubular capillaries in the rat kidney. Evaluation of the counter-current mechanism between the proximal convoluted tubules and the peritubular capillaries in the rat nephronP Faarup
University Institute of Molecular Pathology, Copenhagen, Denmark
APMIS 108:779-84. 2000..We therefore developed a morphological technique suitable for the quantitative evaluation of a counter-current system between the proximal convoluted tubules and the peritubular capillaries in rat renal cortex...
- Electron tomography and immuno-labeling of Tetrahymena thermophila basal bodiesThomas H Giddings
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
Methods Cell Biol 96:117-41. 2010..Collectively, these methods facilitate studies of the mechanism of basal body assembly, the functions of basal body constituents and the cytological role of the basal body as a whole...
- Immunoelectron microscopy for locating calvin cycle enzymes in the thylakoids of synechocystis 6803Rachna Agarwal
Molecular Biology Division, Bhabha Atomic Research Center, Mumbai 400085, India
Mol Plant 2:32-42. 2009Unicellular cyanobacteria Synechocystis 6803 were fixed using high-pressure freezing (HPF) and freeze substitution without any chemical cross-linkers...
- Membrane associated qualitative differences in cell ultrastructure of chemically and high pressure cryofixed plant cellsBernd Zechmann
University of Graz, Institute of Plant Sciences, 8010 Graz, Austria
J Struct Biol 158:370-7. 2007Membrane contrast can sometimes be poor in biological samples after high pressure freezing (HPF) and freeze substitution (FS). The addition of water to the FS-medium has been shown to improve membrane contrast in animal tissue and yeast...
- Ultrastructural localization of amiloride-sensitive sodium channels and Na+,K(+)-ATPase in the rat's olfactory epithelial surfaceB P Menco
Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208 3520, USA
Chem Senses 23:137-49. 1998..This regulation of the salt concentration of an ambient fluid environment is a function that the olfactory epithelium shares with cells of transporting epithelia, such as those of kidney...
- Patterns of asexual reproduction in Nannochloris bacillaris and Marvania geminata (Chlorophyta, Trebouxiophyceae)Maki Yamamoto
Institute of Natural Sciences, Senshu University, 2 1 1, Tama, Kawasaki, Kanagawa 214 8580, Japan
Planta 226:917-27. 2007..We then compared the two algae by transmission electron microscopy with rapid freeze fixation and freeze substitution; in both algae, the mother cell wall was cleaved at the site of cell division, but remained adhered to the ..
- Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium, Shewanella livingstonensis Ac10Jun Kawamoto
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
J Bacteriol 191:632-40. 2009....
- Stabilization of frozen Lactobacillus delbrueckii subsp. bulgaricus in glycerol suspensions: Freezing kinetics and storage temperature effectsF Fonseca
UMR Genie et Microbiologie des Procedes Alimentaires, Institut National de la Recherche Agronomique, F 78850 Thiverval Grignon, France
Appl Environ Microbiol 72:6474-82. 2006..Samples were examined at -196 degrees C and -20 degrees C by freeze fracture and freeze substitution electron microscopy...
- 97-kDa linear IgA bullous dermatosis (LAD) antigen localizes to the lamina lucida of the epidermal basement membraneA Ishiko
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan
J Invest Dermatol 106:739-43. 1996..A post-embedding method with cryofixation and freeze substitution failed to immunolabel the 97-kDa LAD antigen...
- The ultrastructural composition of basement membranes in vivoN Miosge
University of Goettingen, Department of Histology, Germany
Histol Histopathol 16:1239-48. 2001..This preserves the basement membrane with a quality similar to freeze substitution. The application of two antibodies directed toward the C- and N-terminal ends of a molecule and coupled to ..
- Character, distribution and biological implications of ice crystallization in cryopreserved rabbit ovarian tissue revealed by cryo-scanning electron microscopyRoger G Gosden
Center for Reproductive Medicine and Infertility, Weill Medical College of Cornell University, 1305 York Avenue, New York, NY 10021, USA
Hum Reprod 25:470-8. 2010..To address this, we used cryo-scanning electron microscopy (cryo-SEM) to study ice formation in cryopreserved ovarian tissue...
- High-resolution ultrastructural study of the rat glomerular basement membrane in long-term experimental diabetesS Inoue
Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
Ultrastruct Pathol 19:175-85. 1995..By using the freeze substitution method for the preparation of the renal tissue, it was possible to observe that the glomerular basement ..
- In situ molecular identification of the Ntf4 MAPK expression sites in maturing and germinating pollenMaria Jose Coronado
Plant Development and Nuclear Organization Unit, Biological Research Centre CIB, CSIC, C Ramiro de Maeztu 9, 28040 Madrid, Spain
Biol Cell 99:209-21. 2007..However, nothing is known about its subcellular localization...
- Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitutionI L van Genderen
Department of Cell Biology, Medical School, University of Utrecht, The Netherlands
J Cell Biol 115:1009-19. 1991..The present method of lipid immunolabeling holds great potential for the localization of other antigenic lipids...
- Histological analysis of the sublingual gland in rats with streptozotocin-induced diabetesMasaki Kamata
First Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University
Okajimas Folia Anat Jpn 84:71-6. 2007..These findings suggest that the sublingual gland mucous cells become dysfunctional during the development of insulin-dependent diabetes mellitus, although to a slighter degree than the serous demilune cells...
- The spindle pole body of the pathogenic yeast Cryptococcus neoformans: variation in morphology and positional relationship with the nucleolus and the bud in interphase cellsMasashi Yamaguchi
Medical Mycology Research Center, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8673, Japan
J Electron Microsc (Tokyo) 59:165-72. 2010..The present study highlights the necessity of observing a large number of micrographs in three dimensions to describe accurately the ultrastructure of the SPB in yeast...
- Monoclonal antibody marker for olfactory sustentacular cell microvilliS K Pixley
Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati College of Medicine, Ohio 45267 0521, USA
Anat Rec 248:307-21. 1997..The olfactory epithelial sustentacular cells may support the survival and function of olfactory receptor neurons, but few reagents are available to mark and manipulate such cells...
- Three-dimensional reconstruction of living mouse liver tissues using cryotechniques with confocal laser scanning microscopyYurika Saitoh
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo City, Yamanashi 409 3898, Japan
J Electron Microsc (Tokyo) 59:513-25. 2010..preserved in paraffin-embedded sections prepared by in vivo cryotechnique (IVCT) and cryobiopsy followed by freeze substitution fixation...
- Morphometry of methacholine-induced bronchoconstriction in the ratD H Eidelman
Meakins Christie Laboratories, Royal Victoria Hospital, Montreal, Quebec, Canada
J Appl Physiol (1985) 75:1702-10. 1993..Lungs were rapidly frozen in liquid N2 30 s after aerosolization and processed with freeze substitution. Using midsagittal slices of left lung, we measured airway narrowing as the ratio of lumen area (aBM) ..
- Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamilyJames Matsunaga
Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA, USA
Mol Microbiol 49:929-45. 2003..High-pressure freeze substitution followed by immunocytochemical electron microscopy confirmed that the Lig proteins were localized to the ..
- Histochemical approach of cryobiopsy for glycogen distribution in living mouse livers under fasting and local circulation loss conditionsYurika Saitoh
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo City, Yamanashi, Japan
Histochem Cell Biol 133:229-39. 2010..Thus, cryobiopsy combined with FS enabled us to examine time-dependent changes in glycogen distribution in the liver tissues of living mice. This combination might be applicable to the clinical evaluation of human liver tissues...
- Field-emission scanning electron microscopy of the internal cellular organization of fungiW H Muller
Department of Molecular Cell Biology, EMSA, Utrecht University, The Netherlands
Scanning 22:295-303. 2000..were macerated in diluted osmium tetroxide to remove the cytoplasmic matrix and subsequently dehydrated by freeze substitution in methanol...
- Transmission electron microscopy of yeastR Wright
University of Washington, Department of Zoology, Seattle, WA 98195 1800, USA
Microsc Res Tech 51:496-510. 2000..b>Freeze substitution methods continue to provide the highest quality of fixation, but equipment needed for these protocols is ..
- Review article fine structure of the exocrine cells of rat sublingual gland revealed by rapid freezing and freeze substitution methodS Yamashina
Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Japan
Eur J Morphol 38:213-8. 2000The ultrastructure of mucous cells of rat sublingual gland processed by rapid freezing, followed by freeze substitution, was compared with that obtained by the standard chemical fixation technique...
- Freezing and cryoprotective dehydration in an Antarctic nematode (Panagrolaimus davidi) visualised using a freeze substitution techniqueD A Wharton
Department of Zoology, University of Otago, P O Box 56, Dunedin, New Zealand
Cryobiology 50:21-8. 2005..davidi, an Antarctic nematode that can survive intracellular ice formation, was visualised using a freeze substitution technique and transmission electron microscopy...
- Toward visualization of nanomachines in their native cellular environmentJason Pierson
Division of Cell Biology, The Netherlands Cancer Institute Antoni van Leeuwenhoek Hospital NKI AVL, Plesmanlaan 121 B6, 1066 CX, Amsterdam, The Netherlands
Histochem Cell Biol 132:253-62. 2009..Here, we review methodological considerations for visualizing nanomachines in a close-to-physiological, cellular context. EM is in a renaissance, and further innovations and training in this field should be fully supported...
- In situ reverse transcription: the magic of strength and anonymityAnna Ligasová
Laboratory of Cell Biology, Institute of Experimental Medicine, v v i, AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic
Nucleic Acids Res 38:e167. 2010..When the high pressure freezing technique followed by the freeze substitution is employed for the cell's preparation, the ratio is higher than 80.
- Ultrastructural changes during desiccation of the anhydrobiotic nematode Ditylenchus dipsaciD A Wharton
Department of Zoology, University of Otago, Dunedin, New Zealand
Tissue Cell 30:312-23. 1998..dipsaci were followed and quantified after preparation of material at different levels of hydration using freeze substitution techniques...
- Three-dimensional distributions of elements in biological samples by energy-filtered electron tomographyR D Leapman
Division of Bioengineering and Physical Science, ORS, National Institutes of Health, Building 13, 3N17, Bethesda, MD 20892, USA
Ultramicroscopy 100:115-25. 2004..Although a high electron dose of approximately 10(7)e/nm2 was required to record two-axis tilt series, specimens were sufficiently stable to allow image alignment and tomographic reconstruction...
- [Ultrastructure and elemental composition of frog bladder granular epithelial cells in normal state and upon stimulation of water transport]A N Gorshkov
Institute of Cytology RAS, St Petersburg
Tsitologiia 42:1113-24. 2000....
- Plastic-embedded protein crystalsRaimond B G Ravelli
EMBL Grenoble, 6 rue Jules Horowitz, BP 181, 38042 Grenoble Cedex 9, France
J Synchrotron Radiat 14:128-32. 2007Rapid vitrification followed by the replacement of the vitrified water by a solvent (freeze substitution) and then resin is a widely used procedure for preparing biological samples for electron microscopy...
- Electron microscopic examination of uncultured soil-dwelling bacteriaKazunobu Amako
Department of Bacteriology, Faculty of Medical Science, Kyushu University, Fukuoka, Japan
Microbiol Immunol 52:265-9. 2008..by culturing under low-temperature, low-nutrient conditions, similar to those found in some natural environments. These unusual morphologies are therefore hypothesized to be characteristic of bacteria in resting or dormant stages...
- Tissue viscance during induced constriction in rabbit lungs: morphological-physiological correlationsT Nagase
Meakins Christie Laboratories, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada
J Appl Physiol (1985) 73:1900-7. 1992..The lungs were then frozen in situ with liquid nitrogen (PEEP = 5 cmH2O), excised, and processed using freeze substitution techniques...
- Changes in surface features during desiccation of the anhydrobiotic plant parasitic nematode Ditylenchus dipsaciDavid A Wharton
Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand
Tissue Cell 34:81-7. 2002..b>Freeze substitution and critical-point drying produced artifacts that obscured changes produced by the desiccation of the ..
- Structural maturation of rubella virus in the Golgi complexCristina Risco
Department of Structure of Macromolecules, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
Virology 312:261-9. 2003..These associations could facilitate the transfer of viral genomes from the cytopathic vacuoles to the areas of rubella assembly in Golgi membranes...
- A unique program for cell death in xylem fibers of Populus stemCharleen L Courtois-Moreau
Umea Plant Science Centre, Department of Plant Physiology, Umea University, 90187 Umea, Sweden
Plant J 58:260-74. 2009..of the cytoplasmic contents, as detected by electron microscopy of samples fixed by high-pressure freezing/freeze substitution (HPF-FS), was gradual and resulted in complete loss of the cytoplasmic contents well before the loss of ..
- Examination of murine tear filmCuong H Tran
Department of Optometry and Vision Sciences, Cardiff University, Cardiff, Wales, United Kingdom
Invest Ophthalmol Vis Sci 44:3520-5. 2003..To define spatially any free aqueous layer in murine tear film...
- Transmission electron microscopy of the bacterial nucleoidMikhail Eltsov
Laboratoire d Analyse Ultrastructurale, Universite de Lausanne, Lausanne, Switzerland
J Struct Biol 156:246-54. 2006....
- ELCS in ice: cryo-electron microscopy of nuclear envelope-limited chromatin sheetsMikhail Eltsov
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstr 1, 69117, Heidelberg, Germany
Chromosoma 123:303-12. 2014..1) aldehyde fixation/dehydration/plastic embedding/sectioning and staining, (2) high-pressure freezing/freeze substitution into plastic/sectioning and staining, and (3) high-pressure freezing/cryo-sectioning/cryo-electron ..
- High-resolution 3D quantitative analysis of caveolar ultrastructure and caveola-cytoskeleton interactionsTobias Richter
Institute for Molecular Bioscience, Queensland Bioscience Precinct, The University of Queensland, Brisbane, Queensland 4072, Australia
Traffic 9:893-909. 2008..The complexity of connections among caveolae and to the actin cytoskeleton and microtubules suggests that individual caveolae may be interconnected through a complex filamentous network to form a single functional unit...
- High Pressure Freezer for Improved UltrastructureDavid Hall; Fiscal Year: 2003..Many of these experiments will utilize immunogold techniques following HPF, freeze substitution and embedding in low temperature resins to localize proteins or mRNA in various tissues and cell types, all ..
- A High-Pressure Freezing and Cryoprocessing System for Northwestern UniversityWILLIAM RUSSIN; Fiscal Year: 2007unreadable] DESCRIPTION (provided by applicant): Cryoimmobilization followed by freeze substitution gives vastly improved ultrastructure and preservation of antigenicity in most cell types when compared to traditional processing ..
- Mitochrondrial Regulation of ApoptosisTomomi Kuwana; Fiscal Year: 2013..We will use high-pressure freezing/freeze substitution-EM to demonstrate the existence of these pores in OMVs and mitochondria both in isolation and in cells...
- EM STUDIES ON MUSCLEKENNETH ALLEN TAYLOR; Fiscal Year: 2013..Specimen preparation emphasizes rapid freezing and freeze substitution which traps molecular motions with millisecond time resolution...
- Cryo Upgrade of UMB EM Core FacilityRu Ching Hsia; Fiscal Year: 2010..the purchase of cryo-EM sample preparation equipment, including a high pressure freezer (HPF), an automatic freeze substitution system, a cryo-ultramicrotome, a plunge freezing device and the cryo upgrade of an existing transmission ..
- Electron cryo-microscopy of phage P22Kristin N Parent; Fiscal Year: 2010..Salmonella, employing both electron tomography of samples treated by High Pressure Freezing and Freeze Substitution (HPF/FS) and fluorescence microscopy...
- Request for Leica High-pressure Freezer and Automated Freeze-substitution SystemSCOT CHARLES KUO; Fiscal Year: 2010..As our large group of EM users (>100 investigators) learn of the advantages of high-pressure freezing, we expect this instrumentation to play a critical role for many more NIH-funded projects. ..
- A MULTI-SCALE APPROACH TO CELL STRUCTURE &FUNCTIONAndreas Hoenger; Fiscal Year: 2013..abstract_text> ..
- MECHANISMS OF MICROCYSTIN-LR HEPATOTOXICITYVal Beasley; Fiscal Year: 1993....
- Leica EM Pact High Pressure Freezing InstrumentM Costello; Fiscal Year: 2004..The biological structure is well preserved for subsequent analysis using techniques such as freeze substitution, freeze-fracture-etch and cryo-ultramicrotomy...
- INTEGRATED EM-IMMUNOLABELING INSTRUMENTATIONKathryn Howell; Fiscal Year: 2001..vacuum deposition system configured for electron microscopy, a Bal- Tec Jet Freeze device and a Bal-Tec Freeze substitution unit...
- TOMOGRAPHIC ANALYSIS OF CYTOKINESIS IN ARABIDOPSISL Staehelin; Fiscal Year: 2002..abstract_text> ..
- TOMOGRAPHIC ANALYSIS OF CYTOKINESIS IN ARABIDOPSISL Staehelin; Fiscal Year: 2003..abstract_text> ..
- High Pressure Freezing and Processing UnitVincent Gattone; Fiscal Year: 2005..The automated freeze substitution processor allows these specimens to be uniformly processed...
- PHILIPS CM120 TRANSMISSION ELECTRON MICROSCOPEKent McDonald; Fiscal Year: 1999..pombe yeasts, Drosophila and C. elegans. The lower material will be prepared by high pressure freezing and freeze substitution for optimum preservation of morphology and antigenicity...
- Electron Tomography of the Outer Hair Cell Lateral WallWILLIAM TRIFFO; Fiscal Year: 2009..Specimens will be prepared using high pressure freezing and freeze substitution (HPF/FS), ensuring the highest-fidelity specimens available and eliminating artifacts present in previous ..
- Equipment for Visualization of Fragile Subcellular Detail by Electron MicroscopyMARCIA MILLER; Fiscal Year: 2009..The superiority of preservation of ultrastructural detail in samples prepared by HPF freezing coupled with freeze substitution fixation (FSF) is readily evident, and this method is becoming the standard fixation method for EM...
- CAPILLARY MORPHOLOGY IN PULMONARY EDEMAROBERT MAZZONE; Fiscal Year: 1980..I will use a freeze substitution technique together with a rapid freezing procedure to stabilize and fix lung structure under carefully ..
- MORPHOLOGY AND CYTOPHYSIOLOGY OF SYNAPTIC TRANSMISSIONJOSEPH PYSH; Fiscal Year: 1980..It is hoped that this project will provide a cytological basis for a better understanding of synaptic function. ..
- CRYOELECTRON MICROSCOPE AND CRYO-ULTRAMICROTOMERoger Craig; Fiscal Year: 1993....
- Advanced Microscopy Shared Resource FacilityVictor Friedrich; Fiscal Year: 2006....
- THE STRUCTURAL BASIS OF NEUROSECRETIONJohn Heuser; Fiscal Year: 1980..frozen at the precise moment of neurotransmission will be prepared by the methods of freeze fracture and freeze substitution for examination in the electron microscope...
- SKELETAL MUSCLE CROSSBRIDGESHugh Huxley; Fiscal Year: 1993..This knowledge will percolate in a broad way into research on health related muscle disease and dysfunction...
- MOLECULAR BASIS FOR CLEAVAGEKeigi Fujiwara; Fiscal Year: 1980..Rapid freezing is done using Heuser's apparatus and freeze-substitution will be done by cold acetone in the presence of OsO4. ..
- Equipment for Correlative LM/EM by High Pressure Freezing and TomographyKent McDonald; Fiscal Year: 2007..In turn, this better understanding of mechanism will promote more effective treatments for disease intervention. [unreadable] [unreadable] [unreadable]..
- LEICA EM UC/6 CRYO-ULTRAMICROTOME AND ACCESSORIESKent McDonald; Fiscal Year: 2005..Use by regional investigators will be encouraged in the time remaining after core investigator use. UC Berkeley will provide space, staff, and funds for maintaining the equipment. ..
- MINERALIZATION OF PRIMARY BONEJEFFREY GORSKI; Fiscal Year: 2008..Results of this work should better define the differences between primariy and lamellar bone, as well as aid in the diagnosis and treatment of osteoporosis and atherosclerosis. ..
- Molecular Structure and Function of the Human Kinetochore Outer PlateBruce F McEwen; Fiscal Year: 2010....
- Investigation of the Nodavirus lifecycle in vivoJason Lanman; Fiscal Year: 2006..To investigate at higher resolution the spatial relationship of FHV within cells, tomographic electron microscope images will be used to produce three-dimensional reconstructions of the virus infected cells. ..
- BAG-75: UNIQUE MARKER OF PRIMARY BONE FORMATIONJEFFREY GORSKI; Fiscal Year: 2003..Stimulation of appositional formation by lamellar bone would not be expected to have the same effect. These future functional studies require determination of the BAG-75 cDNA sequence. ..
- A HIGH PRESSURE FREEZER FOR ELECTRON MICROSCOPY USESGINA SOSINSKY; Fiscal Year: 2002..In all of these research projects, the primary goal is to understand the three-dimensional structure of organized cellular components and relate this morphology to their functionality...
- 3D Structural Biology of the Human IsletBRAD MARSH; Fiscal Year: 2006..abstract_text> ..