biological assay

Summary

Summary: A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.

Top Publications

  1. pmc Palytoxin and analogs: biological and ecological effects
    Vitor Ramos
    Marine and Environmental Research Center CIIMAR CIMAR, Porto University, Rua dos Bragas, 289, 4050 123 Porto, Portugal
    Mar Drugs 8:2021-37. 2010
  2. pmc Utilization, retention and bio-efficacy studies of PermaNet in selected villages in Buie and Fentalie districts of Ethiopia
    Messay Fettene
    Aklilu Lemma Institute of Pathobiology, Addis Ababa University, PO Box 1176, Addis Ababa, Ethiopia
    Malar J 8:114. 2009
  3. pmc Development of vegetable farming: a cause of the emergence of insecticide resistance in populations of Anopheles gambiae in urban areas of Benin
    Anges William M Yadouleton
    Centre de Recherche Entomologique de Cotonou, Benin
    Malar J 8:103. 2009
  4. ncbi Assaying prions in cell culture: the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA)
    Sukhvir P Mahal
    Department of Infectology, Scripps Florida, Jupiter, FL, USA
    Methods Mol Biol 459:49-68. 2008
  5. ncbi Validating parameters of a luciferase reporter gene assay to measure neutralizing antibodies to IFNbeta in multiple sclerosis patients
    Regina Lam
    Neuro Immunology Laboratory, University of British Columbia, Vancouver, Canada
    J Immunol Methods 336:113-8. 2008
  6. pmc The E-SCREEN assay as a tool to identify estrogens: an update on estrogenic environmental pollutants
    A M Soto
    Tufts University School of Medicine, Department of Anatomy and Cellular Biology, Boston, MA 02111, USA
    Environ Health Perspect 103:113-22. 1995
  7. pmc Long-term field performance of a polyester-based long-lasting insecticidal mosquito net in rural Uganda
    Albert Kilian
    Malaria Consortium, Development House, 56 64 Leonard Street EC2A 4JX, London, UK
    Malar J 7:49. 2008
  8. ncbi Prion propagation and toxicity in vivo occur in two distinct mechanistic phases
    Malin K Sandberg
    MRC Prion Unit and Department of Neurodegenerative Disease, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UK
    Nature 470:540-2. 2011
  9. pmc Comparative molecular modeling of Anopheles gambiae CYP6Z1, a mosquito P450 capable of metabolizing DDT
    Ting Lan Chiu
    Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 105:8855-60. 2008
  10. ncbi Malaria vector composition and insecticide susceptibility status in Guinea Conakry, West Africa
    S B Vezenegho
    Vector Control Reference Unit, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa
    Med Vet Entomol 23:326-34. 2009

Detail Information

Publications322 found, 100 shown here

  1. pmc Palytoxin and analogs: biological and ecological effects
    Vitor Ramos
    Marine and Environmental Research Center CIIMAR CIMAR, Porto University, Rua dos Bragas, 289, 4050 123 Porto, Portugal
    Mar Drugs 8:2021-37. 2010
    ..In this paper, we review the current knowledge on the effects of PTX and its analogs on different organisms, exposing the impact that these toxins may have in coastal ecosystems...
  2. pmc Utilization, retention and bio-efficacy studies of PermaNet in selected villages in Buie and Fentalie districts of Ethiopia
    Messay Fettene
    Aklilu Lemma Institute of Pathobiology, Addis Ababa University, PO Box 1176, Addis Ababa, Ethiopia
    Malar J 8:114. 2009
    ..Pyrethroid-treated mosquito nets are one of the major tools available for the prevention and control of malaria transmission. PermaNet is a long-lasting insecticide-treated net (LLIN) recommended by WHO for malaria control...
  3. pmc Development of vegetable farming: a cause of the emergence of insecticide resistance in populations of Anopheles gambiae in urban areas of Benin
    Anges William M Yadouleton
    Centre de Recherche Entomologique de Cotonou, Benin
    Malar J 8:103. 2009
    ..This study aims to assess the rapid expansion of urban agriculture especially, its contribution to the emergence of insecticide resistance in populations of Anopheles gambiae...
  4. ncbi Assaying prions in cell culture: the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA)
    Sukhvir P Mahal
    Department of Infectology, Scripps Florida, Jupiter, FL, USA
    Methods Mol Biol 459:49-68. 2008
    ..Assays performed in a more time-consuming end point titration format extend the sensitivity and show that infectivity titers measured in tissue culture and in the mouse are similar...
  5. ncbi Validating parameters of a luciferase reporter gene assay to measure neutralizing antibodies to IFNbeta in multiple sclerosis patients
    Regina Lam
    Neuro Immunology Laboratory, University of British Columbia, Vancouver, Canada
    J Immunol Methods 336:113-8. 2008
    ..8-29.3%, and the intra-assay coefficient of variation was between 6.3-15.2%. The luciferase assay is reliable, appropriately sensitive and requires less time than the currently available NAb methods...
  6. pmc The E-SCREEN assay as a tool to identify estrogens: an update on estrogenic environmental pollutants
    A M Soto
    Tufts University School of Medicine, Department of Anatomy and Cellular Biology, Boston, MA 02111, USA
    Environ Health Perspect 103:113-22. 1995
    ....
  7. pmc Long-term field performance of a polyester-based long-lasting insecticidal mosquito net in rural Uganda
    Albert Kilian
    Malaria Consortium, Development House, 56 64 Leonard Street EC2A 4JX, London, UK
    Malar J 7:49. 2008
    ....
  8. ncbi Prion propagation and toxicity in vivo occur in two distinct mechanistic phases
    Malin K Sandberg
    MRC Prion Unit and Department of Neurodegenerative Disease, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UK
    Nature 470:540-2. 2011
    ..Production of neurotoxic species is triggered when prion propagation saturates, leading to a switch from autocatalytic production of infectivity (phase 1) to a toxic (phase 2) pathway...
  9. pmc Comparative molecular modeling of Anopheles gambiae CYP6Z1, a mosquito P450 capable of metabolizing DDT
    Ting Lan Chiu
    Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 105:8855-60. 2008
    ..These data identify CYP6Z1 as one important target for inhibitor design aimed at inactivating insecticide-metabolizing P450s in natural populations of this malarial mosquito...
  10. ncbi Malaria vector composition and insecticide susceptibility status in Guinea Conakry, West Africa
    S B Vezenegho
    Vector Control Reference Unit, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa
    Med Vet Entomol 23:326-34. 2009
    ..The heterogeneity in species composition and resistance profiles between sites requires vector control interventions to be tailored to each site based on the data collected from ongoing monitoring and surveillance...
  11. pmc Detection of CWD prions in urine and saliva of deer by transgenic mouse bioassay
    Nicholas J Haley
    Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA
    PLoS ONE 4:e4848. 2009
    ..These findings help extend our understanding of CWD prion shedding and transmission and portend the detection of infectious prions in body fluids in other prion infections...
  12. pmc Detection of protease-resistant cervid prion protein in water from a CWD-endemic area
    T A Nichols
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, USA
    Prion 3:171-83. 2009
    ..These data demonstrate detection of very low levels of PrP(CWD) in the environment by sPMCA and suggest persistence and accumulation of prions in the environment that may promote CWD transmission...
  13. pmc Zebrafish bioassay-guided natural product discovery: isolation of angiogenesis inhibitors from East African medicinal plants
    Alexander D Crawford
    Department of Pharmaceutical Sciences, University of Leuven, Leuven, Belgium
    PLoS ONE 6:e14694. 2011
    ..These results suggest that the combination of zebrafish bioassays with analytical chromatography methods is an effective strategy for the rapid identification of bioactive natural products...
  14. pmc Prion strain discrimination in cell culture: the cell panel assay
    Sukhvir P Mahal
    Department of Infectology, Scripps Florida, 5353 Parkside Drive, Jupiter, FL 33458, USA
    Proc Natl Acad Sci U S A 104:20908-13. 2007
    ....
  15. ncbi Insecticide resistance status of Aedes aegypti in 10 localities in Colombia
    Clara B Ocampo
    Centro Internacional de Entrenamiento e Investigaciones Medicas, Carrera, Cali, Colombia
    Acta Trop 118:37-44. 2011
    ..Periodic surveillance of insecticide resistance is necessary in order to maintain effective interventions. This study helped to establish the National Network for the surveillance of the insecticide resistance in Colombia...
  16. pmc In vitro assessments of nanomaterial toxicity
    Clinton F Jones
    Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112 5820, USA
    Adv Drug Deliv Rev 61:438-56. 2009
    ..A diverse portfolio of complementary material characterization tools and bioassays are required to validate nanomaterial properties in physiology...
  17. ncbi Ciguatera incidence and fish toxicity in Okinawa, Japan
    Naomasa Oshiro
    Okinawa Prefectural Institute of Health and Environment, Ozato, Nanjo, Okinawa, Japan
    Toxicon 56:656-61. 2010
    ..None of the 35 samples of L. argentimaculatus was toxic. Nor the L. bohar samples weighing less than 4 kg were toxic. In all toxic samples, CTX1B was detected by LC/MS analysis but CTX3C and 51-hydroxyCTX3C were not...
  18. pmc Quantitative detection and biological propagation of scrapie seeding activity in vitro facilitate use of prions as model pathogens for disinfection
    Sandra Pritzkow
    Transmissible Spongiform Encephalopathies, Project Group Immune Defense and Pathogenesis, Robert Koch Institut, Berlin, Germany
    PLoS ONE 6:e20384. 2011
    ....
  19. ncbi Infectious titres of sheep scrapie and bovine spongiform encephalopathy agents cannot be accurately predicted from quantitative laboratory test results
    Lorenzo Gonzalez
    Animal Health and Veterinary Laboratories Agency AHVLA, AHVLA Lasswade, Pentlands Science Park, Penicuick, Midlothian EH26 0PZ, UK
    J Gen Virol 93:2518-27. 2012
    ..Furthermore, the near inverse correlation between infectious titres and Western blot results (high protease pre-treatment) argues for a dissociation between infectivity and PrP(res)...
  20. pmc Use of murine bioassay to resolve ovine transmissible spongiform encephalopathy cases showing a bovine spongiform encephalopathy molecular profile
    Katy E Beck
    Animal Health and Veterinary Laboratories Agency, Addlestone, Surrey, UK
    Brain Pathol 22:265-79. 2012
    ..Critically, these data confirmed the original differential IHC categorization that these UK-1 and UK-2 cases were not compatible with BSE...
  21. ncbi Microbial biosensors: a review
    Liang Su
    Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, 191 Auditorium Road, Storrs, CT 06269, USA
    Biosens Bioelectron 26:1788-99. 2011
    ..Prospective strategies for the design of future microbial biosensors will also be discussed...
  22. pmc Occurrence of entomopathogenic fungi from agricultural and natural ecosystems in Saltillo, México, and their virulence towards thrips and whiteflies
    Sergio R Sánchez-Peña
    Departamento de Parasitología Agrícola, Universidad Autonoma Agraria Antonio Narro, Saltillo, Coahuila, Mexico
    J Insect Sci 11:1. 2011
    ..anisopliae, exist in the habitats sampled; pathogenicity varied among strains, and some strains possessed significant virulence. Soils in these habitats are reservoirs of diverse strains with potential for use in biocontrol...
  23. pmc Species-independent bioassay for sensitive quantification of antiviral type I interferons
    Thomas Kuri
    Department of Virology, University of Freiburg, D 79008 Freiburg, Germany
    Virol J 7:50. 2010
    ..These assays however are either time-consuming and lack convenient readouts, or they are rather insensitive and restricted to IFN from a particular host species...
  24. ncbi Avoidance of copper contamination by field populations of Daphnia longispina
    Isabel Lopes
    Instituto do Ambiente e Vida, Departamento de Zoologia da Universidade de Coimbra, Largo Marques de Pombal, 3004 517 Coimbra, Portugal
    Environ Toxicol Chem 23:1702-8. 2004
    ..Therefore, avoidance assays should be recommended as a complementary tool in ecological risk assessments and effluent biomonitoring because such assays can provide cost-effective and ecologically relevant information...
  25. ncbi Combined approach of hematological biomarkers and plasma protein SAA for improvement of radiation dose assessment triage in biodosimetry applications
    Natalia I Ossetrova
    AFRRI, SRD, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Health Phys 98:204-8. 2010
    ....
  26. pmc The OECD program to validate the rat uterotrophic bioassay to screen compounds for in vivo estrogenic responses: phase 1
    J Kanno
    National Institute of Health Sciences, Tokyo, Japan
    Environ Health Perspect 109:785-94. 2001
    ..For the next phase of the OECD validation program, both models will be tested against a battery of weak, partial estrogen agonists...
  27. pmc An overview of the PubChem BioAssay resource
    Yanli Wang
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 38:D255-66. 2010
    ..In this work, we describe the PubChem BioAssay database, including data model, bioassay deposition and utilities that PubChem provides for searching, downloading and analyzing the biological activity information contained therein...
  28. ncbi A novel high-throughput screening system to evaluate the behavioral response of adult mosquitoes to chemicals
    John P Grieco
    Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 4799, USA
    J Am Mosq Control Assoc 21:404-11. 2005
    ..The system is compact in size, easy to decontaminate, and requires only a minute quantity of chemical compound...
  29. pmc Microfluidics meet cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays
    Amy L Paguirigan
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Bioessays 30:811-21. 2008
    ..With more validation and improved designs, microscale techniques hold immense promise as a platform to study aspects of cell biology that are not possible using current macroscale techniques...
  30. ncbi A call to standardize teratoma assays used to define human pluripotent cell lines
    Franz Josef Muller
    Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell Stem Cell 6:412-4. 2010
    ..However, reports of new human ESC and iPSC lines vary widely in both methods and analysis of teratoma data. We call for consensus standards to be established to make this assay worthy of its "golden" status...
  31. pmc Reduced bio-efficacy of permethrin EC impregnated bednets against an Anopheles gambiae strain with oxidase-based pyrethroid tolerance
    Josiane Etang
    Institute of Medical Research and Studies of Medicinal Plants IMPM, Ministry of Scientific Research and Technique, P, O, Box 6163, Yaounde, Cameroon
    Malar J 3:46. 2004
    ..The purpose of this study was to evaluate knockdown and killing effects of ITNs on a metabolic-based resistant or tolerant malaria vector strain...
  32. ncbi Activities and identification of aryl hydrocarbon receptor agonists in sediments from the Danube river
    Steffen Keiter
    Aquatic Ecology and Toxicology Section, Department of Zoology, University of Heidelberg, Im Neuenheimer Feld 230, 69120, Heidelberg, Germany
    Anal Bioanal Chem 390:2009-19. 2008
    ..Nevertheless, owing to the effects of PAHs towards fish and the connection between dioxin-like activity and carcinogenicity, the link between contamination and the fish population decline cannot be ruled out...
  33. ncbi Impact of glyphosate and benzo[a]pyrene on the tolerance of mosquito larvae to chemical insecticides. Role of detoxification genes in response to xenobiotics
    Muhammad Asam Riaz
    Laboratoire d Ecologie Alpine LECA, UMR CNRS 5553, Equipe Perturbation Environnementales et Xenobiotiques, France
    Aquat Toxicol 93:61-9. 2009
    ..Overall, this study reveals the potential of benzo[a]pyrene and glyphosate to affect the tolerance of mosquito larvae to chemical insecticides, possibly through the cross-induction of particular genes encoding detoxification enzymes...
  34. ncbi A practical and user-friendly toxicity classification system with microbiotests for natural waters and wastewaters
    Guido Persoone
    Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, J Plateaustraat 22, 9000 Ghent, Belgium
    Environ Toxicol 18:395-402. 2003
    ....
  35. pmc cis-Jasmone induces Arabidopsis genes that affect the chemical ecology of multitrophic interactions with aphids and their parasitoids
    Toby J A Bruce
    Rothamsted Research, Harpenden AL5 2JQ, United Kingdom
    Proc Natl Acad Sci U S A 105:4553-8. 2008
    ....
  36. ncbi Analytical detection and biological assay of antileukemic drug 5-fluorouracil using gold nanoparticles as probe
    Vaithilingam Selvaraj
    Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai 600 025, India
    Int J Pharm 337:275-81. 2007
    ..The experimental studies suggest that gold nanoparticles have the potential to be used as effective carriers for anticancer drugs...
  37. pmc Fast, automated measurement of nematode swimming (thrashing) without morphometry
    Steven D Buckingham
    MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK
    BMC Neurosci 10:84. 2009
    ..Our method uses principal components analysis to remove the background, followed by computation of a covariance matrix of the remaining image frames from which the interval between statistically-similar frames is estimated...
  38. ncbi Resistance of Aedes aegypti to organophosphates in several municipalities in the State of Rio de Janeiro and Espírito Santo, Brazil
    José Bento Pereira Lima
    Laboratório de Transmissores de Hematozoários, Departamento de Entomologia, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Manguinhos, Rio de Janeiro, Brazil
    Am J Trop Med Hyg 68:329-33. 2003
    ..59 to 12.41. Adults from only one municipality (Nova Iguaçu, Rio de Janeiro) remained susceptible to both fenitrothion and malathion. These results are being used to define new local vector control strategies...
  39. pmc Distribution of ace-1R and resistance to carbamates and organophosphates in Anopheles gambiae s.s. populations from Côte d'Ivoire
    Ludovic P Ahoua Alou
    Institut Pierre Richet, BP 47 Abidjan, Cote d Ivoire
    Malar J 9:167. 2010
    ..It is, therefore, essential to investigate on the susceptibility of pyrethroid resistant populations of An. gambiae s.s. to these alternative products...
  40. ncbi Strengths and weaknesses of in vitro assays for estrogenic and androgenic activity
    Ana M Soto
    Department of Anatomy and Cellular Biology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    Best Pract Res Clin Endocrinol Metab 20:15-33. 2006
    ..The development of fast and sensitive bioassays is central to the achievement of these three goals...
  41. pmc Problems and challenges in the development and validation of human cell-based assays to determine nanoparticle-induced immunomodulatory effects
    Gertie J Oostingh
    Department of Molecular Biology, University of Salzburg, 5020 Salzburg, Austria
    Part Fibre Toxicol 8:8. 2011
    ..However, before assays can be standardised, it is important that suitable methods are established and validated...
  42. ncbi Molecular and biochemical characterization of a sand fly population from Sri Lanka: evidence for insecticide resistance due to altered esterases and insensitive acetylcholinesterase
    S N Surendran
    Department of Zoology, University of Jaffna, Jaffna, Sri Lanka
    Bull Entomol Res 95:371-80. 2005
    ....
  43. pmc Immunologic biomarkers in relation to exposure markers of PCBs and dioxins in Flemish adolescents (Belgium)
    Rosette L Van Den Heuvel
    Department of Toxicology, Vito Flemish Institute of Technological Research, Mol, Belgium
    Environ Health Perspect 110:595-600. 2002
    ..The negative correlation between PHAHs and allergic responses in adolescents suggested that exposure may entail alterations in the immune status...
  44. pmc Scrapie Agent (Strain 263K) can transmit disease via the oral route after persistence in soil over years
    Bjoern Seidel
    Fraunhofer Institute for Molecular Biology und Applied Ecology IME, Schmallenberg, Germany
    PLoS ONE 2:e435. 2007
    ..PMCA opens therefore a perspective for considerably improving the detectability of prions in soil samples from the field...
  45. ncbi Continuous human metastin 45-54 infusion desensitizes G protein-coupled receptor 54-induced gonadotropin-releasing hormone release monitored indirectly in the juvenile male Rhesus monkey (Macaca mulatta): a finding with therapeutic implications
    Stephanie B Seminara
    Reproductive Endocrinology Unit, Massachusetts General Hospital, Boston, 02114, USA
    Endocrinology 147:2122-6. 2006
    ..e. precocious puberty, endometriosis, uterine fibroids, and prostate cancer)...
  46. ncbi Detection of okadaic acid and related esters in mussels during diarrhetic shellfish poisoning (DSP) episodes in Greece using the mouse bioassay, the PP2A inhibition assay and HPLC with fluorimetric detection
    Eleanna Prassopoulou
    Department of Food Technology, Technological Educational Institute of Thessaloniki, 54110 Sindos, Greece
    Toxicon 53:214-27. 2009
    ..86 microg OA/kg, which permits this method to be used for the regulatory control of these toxins in shellfish. Comparison of the results by all three methods revealed excellent consistency...
  47. pmc Development and characterization of a novel single-cycle recombinant-virus assay to determine human immunodeficiency virus type 1 coreceptor tropism
    Jeannette M Whitcomb
    Monogram Biosciences, Inc, 345 Oyster Point Boulevard, South San Francisco, CA 94080, USA
    Antimicrob Agents Chemother 51:566-75. 2007
    ....
  48. ncbi Quantitative bioanalytical methods validation and implementation: best practices for chromatographic and ligand binding assays
    C T Viswanathan
    Food and Drug Administration, Center for Drug Evaluation and Research, Rockville, Maryland 20857, USA
    Pharm Res 24:1962-73. 2007
    ..This communication represents the distillate of the workshop proceedings and provides the summary of consensus reached and also contains the validation topics where no consensus was reached...
  49. pmc Adiponectin identified as an agonist for PAQR3/RKTG using a yeast-based assay system
    Ibon Garitaonandia
    Department of Chemistry, University of Florida, Gainesville, FL 32611, USA
    J Recept Signal Transduct Res 29:67-73. 2009
    ..Thus, the interaction between PAQR3 and adiponectin may help explain the antiproliferative properties of adiponectin...
  50. ncbi Mouse bioassay for palytoxin. Specific symptoms and dose-response against dose-death time relationships
    P Riobo
    Grupo de Fitoplancton Tóxico, Instituto Investigacións Mariñas CSIC Eduardo Cabello 6, 36208 Vigo, Galicia, Spain
    Food Chem Toxicol 46:2639-47. 2008
    ..Herein is demonstrated that the logistic equation and the accumulative function of Weibull's distribution (with the modifications proposed) generate satisfactory toxicological descriptions in all the respects...
  51. ncbi Label-free assays on the BIND system
    Brian T Cunningham
    SRU Biosystems, 14A Gill Street, Woburn, Massachusetts 01801, USA
    J Biomol Screen 9:481-90. 2004
    ..Small molecule screening, cell proliferation/cytotoxicity, enzyme activity screening, protein-protein interaction, and cell membrane receptor expression are among the applications demonstrated...
  52. ncbi High-throughput screening of natural products for cancer therapy
    Alan L Harvey
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK
    Planta Med 76:1080-6. 2010
    ..The molecular assays include those directed at microtubules and related proteins and at many different protein kinases...
  53. ncbi Some heterocyclic aromatic compounds are Ah receptor agonists in the DR-CALUX assay and the EROD assay with RTL-W1 cells
    Gunnar Hinger
    Aquatic Toxicology and Ecology Section, Department of Zoology, University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany
    Environ Sci Pollut Res Int 18:1297-304. 2011
    ..However, only few publications are available investigating NSO-HET using in vitro bioassays. To support better characterization of environmental samples, selected NSO-HET were screened for dioxin-like activity in two bioassays...
  54. pmc A simple, versatile and sensitive cell-based assay for prions from various species
    Zaira E Arellano-Anaya
    UMR Institut National de la Recherche Agronomique Ecole Nationale Vétérinaire de Toulouse 1225, Interactions Hôte Agent Pathogène, Toulouse, France
    PLoS ONE 6:e20563. 2011
    ....
  55. pmc A database of reaction monitoring mass spectrometry assays for elucidating therapeutic response in cancer
    Elizabeth R Remily-Wood
    Molecular Oncology and Proteomics, H Lee Moffitt Cancer Center, Tampa, FL 33612, USA
    Proteomics Clin Appl 5:383-96. 2011
    ..moffitt.org/QUAD/, facilitates widespread implementation of quantitative mass spectrometry in cancer biology and clinical research through sharing of methods and reagents for monitoring protein expression and modification...
  56. pmc Detection of chronic wasting disease prions in salivary, urinary, and intestinal tissues of deer: potential mechanisms of prion shedding and transmission
    Nicholas J Haley
    Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
    J Virol 85:6309-18. 2011
    ..These findings offer unique insights into the transmission of CWD in particular and prion infection and trafficking overall...
  57. ncbi Protein–protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, Japan
    FEBS J 277:1982-95. 2010
    ....
  58. ncbi Changes in concentrations of hydrophilic organic contaminants and of endocrine-disrupting potential downstream of small communities located adjacent to headwaters
    B Jarosova
    Research Centre for Toxic Compounds in the Environment RECETOX, Faculty of Science, Masaryk University, Kamenice 126 3, 62500, Brno, Czech Republic
    Environ Int 45:22-31. 2012
    ..Chemical analyses along with the results of bioassays documented impacts of small towns with WWTPs on headwaters...
  59. pmc Membrane blebbing as an assessment of functional rescue of dysferlin-deficient human myotubes via nonsense suppression
    Bingjing Wang
    University of Pennsylvania School of Medicine, Department of Physiology, B400 Richards Bldg, 3700 Hamilton Walk, Philadelphia, PA 19104 6085, USA
    J Appl Physiol (1985) 109:901-5. 2010
    ..Thus ataluren is a potential therapeutic for dysferlin-deficient patients harboring nonsense mutations...
  60. ncbi Zebrafish bioassay-guided microfractionation for the rapid in vivo identification of pharmacologically active natural products
    Soura Challal
    Université de Genève Quai Ernest Ansermet, Geneve 4, Switzerland
    Chimia (Aarau) 66:229-32. 2012
    ..In summary, we present an in vivo , microgram-scale NP discovery platform combining zebrafish bioassays with microscale analytics to identify, isolate and evaluate pharmacologically active NPs...
  61. pmc Single laboratory validation of a ready-to-use phosphatase inhibition assay for detection of okadaic acid toxins
    Henry G F Smienk
    Zeu Inmunotec, Polígono PLAZA, C Bari 25 Dpdo, 50197, Zaragoza, Spain
    Toxins (Basel) 4:339-52. 2012
    ..Finally, the method was qualitatively compared to the mouse bioassay and LC-MS/MS...
  62. pmc Bio-efficacy of selected long-lasting insecticidal nets against pyrethroid resistant Anopheles arabiensis from South-Western Ethiopia
    Delenasaw Yewhalaw
    Department of Biology, College of Natural Sciences, Jimma University, Jimma, Ethiopia
    Parasit Vectors 5:159. 2012
    ..s. and Anopheles arabiensis may compromise control initiatives based on insecticide-treated nets (ITNs) or indoor residual spraying (IRS), and thus threaten the global malaria elimination strategy...
  63. ncbi A novel quantitative flow cytometry-based assay for autophagy
    Kai Er Eng
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Autophagy 6:634-41. 2010
    ..Moreover, using direct antibody staining for the endogenous LC3 protein, we have extended this assay to the detection of autophagosome formation in non-transfected cells...
  64. pmc Infection of cell lines with experimental and natural ovine scrapie agents
    Michael H Neale
    Molecular Pathogenesis and Genetics Department, Veterinary Laboratories Agency, Addlestone, United Kingdom
    J Virol 84:2444-52. 2010
    ..Increasing the sensitivity of the Rov9 cell line to SSBP/1 infection did not correlate with broadening susceptibility, as the specificity of permissiveness and resistance to other scrapie isolates was maintained...
  65. ncbi High throughput kinetic Vibrio fischeri bioluminescence inhibition assay for study of toxic effects of nanoparticles
    M Mortimer
    Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn, Estonia
    Toxicol In Vitro 22:1412-7. 2008
    ..Thus, considering an excellent correlation between both formats, 96-well microplate Flash Assay can be successfully used for high throughput evaluation of harmful properties of chemicals (including organic and inorganic NPs) to bacteria...
  66. ncbi Microtiter-based assay for evaluating the biological activity of ribosome-inactivating proteins
    M L Hale
    Toxinology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702 5011, USA
    Pharmacol Toxicol 88:255-60. 2001
    ..The assay is suitable for efficient in vitro screening of therapeutics, as well as for identifying samples containing ribosome-inactivating proteins...
  67. ncbi A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP
    Michael Halter
    Cell Systems Science Group Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Assay Drug Dev Technol 7:356-65. 2009
    ..Unlike the other assays, monitoring cellular GFP on a per-cell basis allows detection of reduced ribosome activity before significant cell death occurs, and the results are not convoluted by the numbers of cells being assayed...
  68. ncbi Toxicity and detection of ricin and abrin in beverages
    Eric A E Garber
    U S Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, Maryland 20740, USA
    J Food Prot 71:1875-83. 2008
    ..Enzyme-linked immunosorbent assays of the samples administered by i.p. injection indicated a decrease in detectable toxin at 0.5 microg/ml...
  69. ncbi Palytoxins: biological and chemical determination
    Pilar Riobó
    Instituto de Investigaciones Marinas, CSIC, Eduardo Cabello, 6, CP36208 Vigo, Spain
    Toxicon 57:368-75. 2011
    ..This paper reviews all reported biological and chemical analysis methods to date for this group of compounds...
  70. ncbi Fishing for drugs from nature: zebrafish as a technology platform for natural product discovery
    Alexander D Crawford
    Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Leuven, Belgium
    Planta Med 74:624-32. 2008
    ..Here, we review recent natural product research using zebrafish and evaluate the potential of this vertebrate model as a discovery platform for the systematic identification of bioactive natural products...
  71. ncbi The validity of two HPLC methods and a colorimetric PP2A assay related to the mouse bioassay in quantification of diarrhetic toxins in blue mussels (Mytilus edulis)
    H Ramstad
    Department of Pharmacology, Microbiology and Food Hygiene, Norwegian School of Veterinary Science, PO Box 8146, Oslo, Norway
    Toxicon 39:1387-91. 2001
    ..All three methods could replace the mouse bioassay in detecting levels of diarrhetic toxins approved internationally for safe consumption of mussels. However, HPLC-ADAM seems to be the method of choice...
  72. ncbi Fate of Ah receptor agonists during biological treatment of an industrial sludge containing explosives and pharmaceutical residues
    Lillemor K Gustavsson
    Man Technology Environment Research Centre MTM, Department of Natural Science, University of Orebro, S 70182 Orebro, Sweden
    Environ Sci Pollut Res Int 11:379-87. 2004
    ..Therefore, we studied the sludge before and after the treatments in order to detect any changes in levels of Ah receptor (AhR) agonists using two bioassays for dioxin-like compounds...
  73. ncbi A bioassay approach to determine the dioxin-like activity in sediment extracts from the Danube River: ethoxyresorufin-O-deethylase induction in gill filaments and liver of three-spined sticklebacks (Gasterosteus aculeatus L.)
    Jens C Otte
    Department of Environmental Toxicology, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden
    Environ Int 34:1176-84. 2008
    ..Implementation of the EROD assay in testing of sediment extracts gave highly reliable results which make this assay an ecotoxicologically relevant method for assessment of contamination with Ah receptor agonists in sediments...
  74. ncbi Monitoring of environmental pollutants by bioluminescent bacteria
    Stefano Girotti
    Department of Metallurgic Science, Electrochemistry and Chemical Techniques, University of Bologna, Via S Donato 15, 40127 Bologna, Italy
    Anal Chim Acta 608:2-29. 2008
    ..Comparison to other bioindicators and bioassay has been also made. Various tables have been inserted, to make easier to take a rapid glance at all possible references concerning the topic of specific interest...
  75. ncbi Identification of a small molecule that induces mitotic arrest using a simplified high-content screening assay and data analysis method
    Christopher J Wilson
    Department of Chemical Genomics, ArQule Incorporated, Woburn, MA, USA
    J Biomol Screen 11:21-8. 2006
    ..The active compound, a quinazolinone originating from a natural product-like subset of the screened compounds, is active in cells at approximately 500 nM and appears to act by inhibiting the polymerization of tubulin...
  76. ncbi Androgen receptor transactivation assay using green fluorescent protein as a reporter
    Verena Beck
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria
    Anal Biochem 373:263-71. 2008
    ..The newly developed androgen receptor transactivation assay is a useful tool for characterizing compounds with androgenic activity...
  77. ncbi High-throughput assay for small molecules that modulate zebrafish embryonic heart rate
    C Geoffrey Burns
    Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA
    Nat Chem Biol 1:263-4. 2005
    ..This is the first high-throughput micro-well assay for organ function in an intact vertebrate...
  78. pmc Cell-free formation of misfolded prion protein with authentic prion infectivity
    Petra Weber
    Center for Neuropathology and Prion Research, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 23, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 103:15818-23. 2006
    ....
  79. ncbi Occurrence of palytoxin-group toxins in seafood and future strategies to complement the present state of the art
    Katerina Aligizaki
    Department of Botany, School of Biology, Aristotle University of Thessaloniki, PO Box 109, Thessaloniki 54124, Greece
    Toxicon 57:390-9. 2011
    ..Future recommendations include systematic screening of PlTX in those species and areas where PlTX has already been recorded implementing updated methodologies...
  80. ncbi Multiple parameter radiation injury assessment using a nonhuman primate radiation model-biodosimetry applications
    William F Blakely
    Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603 USA
    Health Phys 98:153-9. 2010
    ..These results demonstrate the practical use of multiple parameter biomarkers to enhance the discrimination of exposed vs. non-exposed individuals and justify a follow-on rhesus macaque dose-response study...
  81. ncbi Toxicity identification evaluation of leachates from municipal solid waste landfills: a multispecies approach
    Marina Isidori
    Dipartimento di Scienze della Vita, Seconda Universita di Napoli, Via A Vivaldi, 43 I 81100, Caserta, Italy
    Chemosphere 52:85-94. 2003
    ..Furthermore, results showed that toxicants could be characterized as cations, basic chemicals, suspended solids and apolar compounds...
  82. ncbi Attomolar sensitivity in bioassays based on surface plasmon fluorescence spectroscopy
    Fang Yu
    Max Planck Institute for Polymer Research, Ackermannweg 10, D 55128 Mainz, Germany
    J Am Chem Soc 126:8902-3. 2004
    ..Time-resolved ultratrace detection of fluorophore (Alexa-Fluor 647)-labeled rabbit anti-mouse antibody down to 500 aM (10-18 M) was accomplished, corresponding to a binding rate of approximately 10 molecules mm-2 min-1...
  83. ncbi Isolation of a Lysinibacillus fusiformis strain with tetrodotoxin-producing ability from puffer fish Fugu obscurus and the characterization of this strain
    Jun Wang
    Department of Life Sciences, Binzhou University, 391 Huanghe Road 5, Binzhou 256600, China
    Toxicon 56:640-3. 2010
    ..On the basis of the physiological and biochemical characteristics of this strain and the results of 16S rRNA analysis, strain B-1 was identified as Lysinibacillus fusiformis...
  84. ncbi Relative potencies of individual polycyclic aromatic hydrocarbons to induce dioxinlike and estrogenic responses in three cell lines
    D L Villeneuve
    Department of Zoology, National Food Safety and Toxicology Center, Institute for Environmental Toxicology, Michigan State University, East Lansing, Michigan 48824, USA
    Environ Toxicol 17:128-37. 2002
    ..Overall, this research contributes to the growing consensus regarding the dioxinlike potency of priority PAHs and PAH derivatives and provides some additional evidence about potentially estrogenic PAHs...
  85. ncbi Susceptibility to chemical insecticides of two Brazilian populations of the visceral leishmaniasis vector Lutzomyia longipalpis (Diptera: Psychodidae)
    B Alexander
    Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerias, Brazil
    Trop Med Int Health 14:1272-7. 2009
    ..e. no exposure and repeated exposure, respectively)...
  86. ncbi Wash-resistance and field evaluation of alphacypermethrin treated long-lasting insecticidal net (Interceptor) against malaria vectors Anopheles culicifacies and Anopheles fluviatilis in a tribal area of Orissa, India
    Surya K Sharma
    National Institute of Malaria Research, Field Station, Sector 5, Rourkela 769 002, Orissa, India
    Acta Trop 116:24-30. 2010
    ..The results of the study showed that Interceptor nets are effective against the malaria vectors and may be used as a suitable intervention strategy in high-risk areas...
  87. ncbi Effect of root-derived substrates on the expression of nah-lux genes in Pseudomonas fluorescens HK44: implications for PAH biodegradation in the rhizosphere
    Roopa Kamath
    Department of Civil and Environmental Engineering, The University of Iowa, Iowa City, Iowa 52242, USA
    Environ Sci Technol 38:1740-5. 2004
    ..This could explain the faster PAH degradation commonly reported in planted than in unplanted soils...
  88. ncbi Urine testing for designer steroids by liquid chromatography with androgen bioassay detection and electrospray quadrupole time-of-flight mass spectrometry identification
    Michel W F Nielen
    RIKILT Institute of Food Safety, P O Box 230, 6700 AE Wageningen, The Netherlands
    Anal Chem 78:424-31. 2006
    ....
  89. ncbi Differential effects of prion particle size on infectivity in vivo and in vitro
    Petra Weber
    Center for Neuropathology and Prion Research, Ludwig Maximilians University of Munich, Feodor Lynen Strasse 23, 81377 Munich, Germany
    Biochem Biophys Res Commun 369:924-8. 2008
    ..e. sonicated samples) the effect of NC-adsorption on clearance dominates resulting in increased infectivity...
  90. ncbi Sediments are major sinks of steroidal estrogens in two United Kingdom rivers
    Mika Peck
    University of Sussex, Department of Chemistry, Physics and Environmental Science, Falmer, Brighton BN1 9QJ, United Kingdom
    Environ Toxicol Chem 23:945-52. 2004
    ..These results suggest that riverine sediments are a major sink and a potential source of persistent estrogenic contaminants...
  91. ncbi Wash resistance of PermaNets in comparison to hand-treated nets
    K Gunasekaran
    Vector Control Research Centre, Medical Complex, Indira Nagar, Pondicherry 605006, India
    Acta Trop 105:154-7. 2008
    ..The relationship of 80% mortality from a 3min bioassay to effectiveness against free flying mosquitoes remains to be determined...
  92. ncbi Evaluation of high-throughput genotoxicity assays used in profiling the US EPA ToxCast chemicals
    Andrew W Knight
    Gentronix Ltd, CTF Building, Manchester, M13 9NT, UK
    Regul Toxicol Pharmacol 55:188-99. 2009
    ....
  93. ncbi Persistence and wash-resistance of insecticidal efficacy of nettings treated with deltamethrin tablet formulation (K-O TAB) against malaria vectors
    Rajendra M Bhatt
    Malaria Research Centre, Field Station, Civil Hospital, Nadiad 387001, Gujarat, India
    J Am Mosq Control Assoc 21:54-8. 2005
    ..Treated polyester nets were found most effective. Compared with our earlier experiences of using liquid formulations, the tablet formulation is likely to have a better community acceptance in treating nets...
  94. ncbi Investigation by bioassay of the efficacy of sodium hydroxide treatment on the inactivation of mouse-adapted scrapie
    A Unal
    CSL Bioplasma, 189 209 Camp Road, Broadmeadows, Victoria 3047, Australia
    Biologicals 35:161-4. 2007
    ..Use of 0.5M NaOH at 30 degrees C for 60 or 75min was found to inactivate >or=4.23 and 4.15logs of scrapie. This indicates that the use of these conditions in an industrial process would substantially reduce prion infectivity...
  95. ncbi Ionworks HT: a new high-throughput electrophysiology measurement platform
    Kirk Schroeder
    Essen Instruments, Ann Arbor, MI 48108, USA
    J Biomol Screen 8:50-64. 2003
    ..5) exogenously expressed in CHO cells are presented. The IonWorks HT device can be used in biophysical and pharmacological profiling of ion channels in an environment compatible with high-capacity screening...
  96. ncbi Evaluation of mutagenic activities of leachates in landfill sites by micronucleus test and comet assay using goldfish
    Yuya Deguchi
    Graduate School of Nutritional and Environmental Sciences and COE Program in the 21st Century, University of Shizuoka, 52 1 Yada, Shizuoka 422 8526, Japan
    Mutat Res 627:178-85. 2007
    ..A combination of the two bioassays was shown to be useful to evaluate the mutagenic activity of the leachates. We also propose a new scoring method for determination of water quality by using acute toxicity and mutagenic activity...
  97. pmc Screening for pharmacological chaperones in Fabry disease
    Sang Hoon Shin
    Developmental and Metabolic Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 10 Center Drive, Building 10, Room 3D04, MSC 1260, Bethesda, MD 20892 1260, USA
    Biochem Biophys Res Commun 359:168-73. 2007
    ..This assay method has general utility in other disorders in assessing the degree of enhancement of activity of mutated proteins by PCT...
  98. pmc Direct detection of soil-bound prions
    Sacha Genovesi
    Dipartimento di Chimica Biologica, Universita di Padova, Padova, Italy
    PLoS ONE 2:e1069. 2007
    ..g. it can currently test only a few mouse prion strains, the cell model can nevertheless be applied in its present form to understand how soil composition influences infectivity, and to test prion-inactivating procedures...
  99. ncbi A bioluminescence assay for thyrotropin receptor antibodies predicts serum thyroid hormone levels in patients with de novo Graves' disease
    G C J Hovens
    Department of Endocrinology, Leiden University Medical Center, Leiden, Netherlands
    Clin Endocrinol (Oxf) 64:429-35. 2006
    ..In commercially available assays however, only total TBII titres can be measured, without discriminating between TSAb and TBAb...
  100. ncbi Two novel species of tetrodotoxin-producing bacteria isolated from toxic marine puffer fishes
    Chun Fai Yu
    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hunghom, Kowloon, Hong Kong, China
    Toxicon 44:641-7. 2004
    ..3 to 105.3 mouse units (MU) in 500 ml of broth medium by mouse bioassay. The principal toxic component in the bacterial cultures was determined to be TTX by thin layer chromatography and mass spectrometry...
  101. ncbi Reporter gene assays and their applications to bioassays of natural products
    D C New
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, China
    Phytother Res 17:439-48. 2003
    ..The different types of intracellular and extracellular reporter gene products and their potential applications in bioassays of natural products are also discussed...

Research Grants77

  1. MULTIPLE-SCLEROSIS: A CD40 LIGAND ANTAGONIST
    Lloyd Kasper; Fiscal Year: 2005
    ..We will also develop a novel biological assay to determine efficacy of therapy(secondary outcome)...
  2. Role of Polyamines in MYCN-Amplified Neuroblastoma
    ANDRE BACHMANN; Fiscal Year: 2009
    ..Our research will be useful in the development of novel treatments for the most aggressive forms of the childhood cancer neuroblastoma. ..
  3. Structure and Function of the Hepatitis C Virus Genome
    Peter Sarnow; Fiscal Year: 2013
    ..To do this, novel biochemical, structural and genetic approaches will be employed to study protein-HCV RNA interactions. ..
  4. A Targeted Preemptive Approach to Addressing Mitochondrial Toxicity of Nucleoside
    John K Buolamwini; Fiscal Year: 2013
    ..Novel research tools will also be developed. ..
  5. Polymorphisms of estrogen-metabolizing genes in kidney cancer
    Yuichiro Tanaka; Fiscal Year: 2013
    ..Such information can lead to the identification of Veterans with higher risk for RCC. Proposed research may also lead to implications for possible therapy for RCC. ..
  6. Stem cells and the fibroblast/adipocyte lineage in arrhythmogenic cardiomyopathy
    Karen L Vikstrom; Fiscal Year: 2010
    ..To understand the role of these stem cells in ARVC, and potentially manipulate their function to preserve the heart wall, are the main goals of this grant. ..
  7. Dynamics of Transcriptional Regulation
    Elena Zelin; Fiscal Year: 2011
    ..Hence, investigation of molecular mechanisms of transcriptional control will potentially facilitate design of drug-and gene-based therapeutic strategies to treat these abnormalities. ..
  8. Molecular Basis of Bacterial Quorum Sensing Gene Regulation
    MAIR E CHURCHILL; Fiscal Year: 2010
    ..abstract_text> ..
  9. Neurotoxicity of organophosphorus pesticides in developing guinea pigs
    William Randall; Fiscal Year: 2013
    ....
  10. Social Regulation of Gene Expression
    John T Cacioppo; Fiscal Year: 2013
    ..The proposed research therefore is designed to identify the specific biological mechanisms mediating these genomic effects. ..
  11. Characterization of male germline stem cells in a rhesus model of infertility
    Kyle E Orwig; Fiscal Year: 2012
    ..In preliminary studies, we developed primate- to-nude mouse xenotransplantation as a biological assay to evaluate SSC function...
  12. Complex Nanocomposites for Bone Regeneration
    Antoni P Tomsia; Fiscal Year: 2013
    ..The present standard of care for such defects may be altered to eliminate bone grafts, decrease risks to patients, improve quality of life, and increase the armamentarium of the clinician. ..
  13. Role of msps and tacc during axon guidance
    LAURA A LOWERY; Fiscal Year: 2010
    ..Finally, the proteins studied here are also misregulated in certain cancers. Thus, the research proposed here is of broad biomedical significance. ..
  14. Understanding the role of PCNA in DNA mismatch repair subpathways
    EVA MARIE GOELLNER; Fiscal Year: 2013
    ..This mechanistic data will be used to guide future experiments into how PCNA mutations and MMR sub pathway defects influence human tumor formation and chemotherapy resistance. ..
  15. Niche-induced Signaling in Progenitor B Cell Development
    LESLIE ERIC SILBERSTEIN; Fiscal Year: 2013
    ..A better definition of environmental signals controlling B cell development and function will have important implications for research in pathogenesis and treatment of B cell disorders including leukemia and immunodeficiency. ..
  16. Translational approaches to multilevel models of prenatal exposure to cigarettes
    Vanja Dukic; Fiscal Year: 2010
    ....
  17. A Novel Approach to Decoding Vertebrate Gene Regulation
    SAMANTHA JEAN RIESENFELD; Fiscal Year: 2013
    ..In addition, it will pave the way for gene therapy by genetically engineering regulatory elements that drive compounds to specific tissues at different time points. ..
  18. Developing small molecule therapeutics for Ebola hemorrhagic fever virus
    Arnab Basu; Fiscal Year: 2013
    ..Identify EBOV inhibitors with in vitro ADME properties suitable for i.v. and oral dosing;and 5. Evaluate EBOV inhibitors in a guinea pig model of EBOV infection. ..
  19. Cell-Cell Signaling in Embryonic and Retinal Development
    Ilaria Rebay; Fiscal Year: 2011
    ....
  20. Development of a Novel TGF-beta Antagonist for Treating Hepatic Fibrosis
    Jung S Huang; Fiscal Year: 2010
    ..The novel TGF-B receptor antagonist developed in this project should be an ideal drug candidate for treating HF in humans. ..
  21. Chaperone-Enabled studies of epigenetic regulation enzymes
    Anthony A Kossiakoff; Fiscal Year: 2013
    ..Mutations in these enzymes have been implicated in a number of diseases. These studies will provide the biological rationale and structural basis for drug development. ..
  22. Integrative Consequences of Hypoxia
    Nanduri R Prabhakar; Fiscal Year: 2013
    ..This proposal examines systemic, cellular, and molecular mechanisms contribufing to cardio-respiratory changes by CIH. ..
  23. Functional significance of amyloid dynamics and deposition in the AD brain
    Gary E Landreth; Fiscal Year: 2013
    ..We propose to test the effects of bexarotene on the phenotypic polarization and phagocytic activity of microglia in vitro. These studies will be extended to the analysis APP/PS1 mice treated with bexarotene. ..
  24. Structure-based discovery and development of HIV-1 gp41 fusion inhibitors
    Miriam Gochin; Fiscal Year: 2013
    ..Drugs in this class would be useful in microbicides to prevent infection, or could be given orally to treat infection as an alternative to the currently used fusion inhibitor Enfuvirtide(R), which must be given intravenously. ..
  25. The Role of Ribosomal RNA Modifications
    Christine S Chow; Fiscal Year: 2012
    ....
  26. Novel method to create knockout rats using endonucleases and spermatagonialstem
    Eric M Ostertag; Fiscal Year: 2011
    ..Thus, if feasibility is demonstrated, this project would benefit many goals of public health by making the production of mutations in the rat that model human diseases readily accessible to the research community. ..
  27. Isolating novel fungi for antibiotic discovery
    Losee Lucy Ling; Fiscal Year: 2012
    ..The purpose of this project is to use our new technology to identify new classes of antibiotics with broad-spectrum activity against both conventional and biodefense pathogens. ..
  28. CONSORTIUM FOR LARGE-SCALE PRODUCTION AND CRYOPRESERVATION KNOCKOUT MICE: AIDS
    MONICA JEAN JUSTICE; Fiscal Year: 2011
    ..Mutant mice generated using ES cell technology are a sensitive biological assay system from which a deep understanding of function can be gleaned and they also provide a long lasting ..
  29. Dysregulation of IGF-1R Translational Control
    Scott W Blume; Fiscal Year: 2013
    ....
  30. Transmitted/Founder HCV clones as targets for treatment and eradication.
    George M Shaw; Fiscal Year: 2013
    ..The proposed work will provide new insights into the molecular biology of HCV replication and persistence and facilitate strategies for eradicating globally circulating HCV genotypes. ..
  31. Characterization and regulation of the MED17-p53 binding interface.
    Zachary C Poss; Fiscal Year: 2013
    ..Elucidation of this interaction will provide a means to potentially regulate p53 activity in cells using either biological or chemical approaches. ..
  32. High throughput screening to find inhibitors of pathogenic pemphigus antibodies
    John R Stanley; Fiscal Year: 2012
    ..Current therapy is to suppress the immune system, which results in many potential adverse effects. This proposal seeks therapy directly targeted only to the autoantibodies that actually cause the blisters in this disease. ..
  33. Rapid, sensitive and labor-free immunoassays for sepsis based on magnetic nanoflu
    Hur Koser; Fiscal Year: 2013
    ..Our approach could easily translate into better antibiotic stewardship and lower mortality rates in sepsis and other severe infections. ..
  34. A Nanofibrous Bioactive Hemodialysis Access Graft
    SAIF G PATHAN; Fiscal Year: 2010
    ....
  35. Exploratory Trial of Choline Supplementation for Fetal Alcohol Syndrome
    Sandra W Jacobson; Fiscal Year: 2013
    ..Maternal and infant nutritional intake will be evaluated by dietary history and biological assay. Infants will be assessed for birth size, growth, and EBC and neurocognitive function at 6...
  36. CONSORTIUM FOR LARGE-SCALE PRODUCTION AND CRYOPRESERVATION OF KNOCKOUT MICE
    FRANCESCO JOHN DEMAYO; Fiscal Year: 2013
    ..Mutant mice generated using ES cell technology are a sensitive biological assay system from which a deep understanding of function can be gleaned and they also provide a long lasting ..
  37. MORPHINE ACTIONS ON THE IMMUNE SYSTEM
    Sulie L Chang; Fiscal Year: 2012
    ..abstract_text> ..
  38. Role of transglutaminase 2 in celiac sprue
    Chaitan Khosla; Fiscal Year: 2013
    ..Sub-Aim 2 seeks to validate their identity in the mouse intestine. Sub-Aim C presents an example of a simple biological assay to understand the role of these post-translational modifications in cell adhesion...
  39. MECHANISMS OF SYNAPTIC PLASTICITY IN THE HIPPOCAMPUS
    Robert C Malenka; Fiscal Year: 2012
    ..In addition, this research will provide information that is critical for the development of agents that modify synaptic transmission in ways that promote cognitive function and alleviate psychiatric symptoms. ..
  40. Correction of defective deltaF508-CFTR processing in cystic fibrosis
    Gergely L Lukacs; Fiscal Year: 2013
    ..Aim 3. Will determine the translational potential of LSDS-based combination ?F508-CFTR corrector therapy by using innovate surrogate human cellular and animal models of CF. ..
  41. Structure-based Optimization of T. brucei methionyl tRNA Synthetase Inhibitors
    Erkang Fan; Fiscal Year: 2013
    ..The goal for this project is to identify one lead and one backup compound that are ready for comprehensive GLP preclinical pharmacology and toxicology studies for further development. ..
  42. Inhibiting protein-primed replication of human enteroviruses.
    Catherine H Schein; Fiscal Year: 2013
    ..Anti-HEV compounds that target uridylylation may thus also play a role in securing polio eradication. ..
  43. Novel Mechanism for Myc-Induced Apoptosis
    CLARE MARIE ADAMS; Fiscal Year: 2013
    ..The education, training, and career development program outlined in this proposal will support my goal of becoming an independent investigator in cancer biology. ! ..
  44. DNA Charge Transport Chemistry &Biology
    Jacqueline K Barton; Fiscal Year: 2012
    ..abstract_text> ..
  45. Stress Responses as Prospective Predictors of Girls'Suicidality and Self-Injury
    Mitchell J Prinstein; Fiscal Year: 2013
    ....
  46. Therapeutic Targeting of Wnt Signaling
    Dianqing Wu; Fiscal Year: 2012
    ..2) To further characterize the Wnt antagonistic compounds and to refine and expand the virtual screen. ..
  47. Restoration of Tumor Suppression Activity in Malignant Melanoma
    DAVID JOSEPH WEBER; Fiscal Year: 2013
    ..SBiXs may also have therapeutic value for treating other cancers with elevated S100B and wt p53 such as astrocytomas, renal tumors, and some forms of leukemia, so this will be explored in future endeavors. ..
  48. Otopathology by Light Microscopy and Molecular Techniques
    Saumil N Merchant; Fiscal Year: 2010
    ..6. To cooperate with the Temporal Bone Research Consortium. This research will provide new knowledge about the causes of disorders of hearing and balance, which should lead to better methods of diagnosis and therapy. ..
  49. NHERF-1 and PTH regulation of the renal transport of phosphate.
    Edward J Weinman; Fiscal Year: 2012
    ..These observations may provide mechanistic insights into the processes that regulate the binding of target proteins to adaptors such as NHERF-1 and provide broader insights into the pathophysiology of NHERF-1 related diseases. ..
  50. Novel Modular Vascular Patterning Assay for HTS
    Royce Mohan; Fiscal Year: 2010
    ....
  51. Discovery of biased small molecule agonists of the GLP-1 receptor for treatment o
    JOHAN KARL ENQUIST; Fiscal Year: 2013
    ....
  52. Center for Undiagnosed Pediatric Renal and Urogenital Disorders
    Nicholas Katsanis; Fiscal Year: 2013
    ....
  53. Integrative Molecular and Phenotype Analysis of 22q11.2 Deletion Syndrome
    Joachim F Hallmayer; Fiscal Year: 2013
    ....
  54. Biology of Imatinib-Resistant Mutants of BCR-ABL
    Michael Deininger; Fiscal Year: 2009
    ..Such crucial proteins may then be exploited as drug targets for the treatment of chronic myeloid leukemia, and further, for other types of cancer. ..
  55. The role of the secretory pathway in ethanol-induced neural tissue injury
    Bing Ye; Fiscal Year: 2013
    ..Thus, it will lay the ground not only for extensive investigation of the role of ethanol on cellular organelles, but also for developing therapeutic strategies to cure ethanol-induced developmental defects. ..
  56. An Improved Diagnostic for Lyme Arthritis
    John E Mueller; Fiscal Year: 2010
    ..burgdorferi outer surface proteins ErpA and ErpP. These peptide ligands will afford us a facile assay to detect B. burgdorferi cells in clinical samples. ..
  57. Genetic Analysis of the PINK1-Parkin Pathway
    Leo J Pallanck; Fiscal Year: 2013
    ..Insight from our studies should be directly relevant to the etiology and treatment of Parkinson's disease, as well as the many other diseases in which mitochondrial dysfunction is implicated. ..
  58. Aggregation Properties of Hemopressin
    Surendra Kumar Nayak; Fiscal Year: 2013
    ..Similarly, if we are able to show that self-assembly and aggregation of HP modulate its pharmacological activity, the finding will have promising therapeutic applicability in drug abuse research. ..
  59. Statistical Methods for Analyzing Antigen Receptors Data
    Grzegorz A Rempala; Fiscal Year: 2013
    ..The statistical methodology derived and deemed most successful will be implemented in the public domain software to be made available at CRAN and caBIG archives. ..
  60. Massively Parallel Identification of Protein Ligands
    Thomas J Kodadek; Fiscal Year: 2013
    ..The synthetic compounds that we plan to identify could also serve as drug leads for a variety of therapeutically interesting targets. ..
  61. Studies of Alternations of c-MET in SCLC
    Patrick Ma; Fiscal Year: 2009
    ..The role of various inhibitors of c-Met and its mutants in SCLC would be further defined aiming to translate into novel targeted-therapeutics. ..
  62. A Functional Census of p53 Cancer and Suppressor Mutants
    Richard H Lathrop; Fiscal Year: 2013
    ..to discover small molecules that induce similar structural changes and test these pre-selected compounds in a biological assay for p53 cancer mutant reactivation...
  63. FUNCTION OF MEF IN HEMATOPOIETIC CELLS
    Stephen Nimer; Fiscal Year: 2011
    ..We hope that the information gained can be exploited to improve the therapeutic index of current cancer treatments and devise new therapeutic approaches. ..
  64. Structure and Function of the Hepatitis C Virus Genome
    PETER contact SARNOW; Fiscal Year: 2010
    ..To do this, novel biochemical, structural and genetic approaches will be employed to study protein-HCV RNA interactions. ..
  65. Novel Extended Red-Shifted Rhodamine Dyes
    NICHOLAS CAIRNS; Fiscal Year: 2004
    ..The dyes will also be tested as components for in vivo optical imaging and a variety of FP and FRET HTS assay kits will be assembled containing the new dyes. ..
  66. A Nonisotopic HTS Assay to Elucidate Choline Transporter (CHT) Modulators
    ALICIA RUGGIERO; Fiscal Year: 2009
    ....
  67. CHARACTERIZATION OF SERUM FACTORS INDUCING MITOSIS
    HARRY ANTONIADES; Fiscal Year: 1980
    ..These two sets of factors, in platelets and PPP, act synergistically to stimulate growth of mammalian cells in culture...
  68. MULTIPLE-SCLEROSIS: A CD40 LIGAND ANTAGONIST
    Lloyd Kasper; Fiscal Year: 2003
    ..We will also develop a novel biological assay to determine efficacy of therapy(secondary outcome)...
  69. URINALYSIS FOR PATIENT COMPLIANCE TO CAM THERAPY
    Rama Ramanujam; Fiscal Year: 2006
    ..Towards this goal, we propose to validate an in vitro biological assay system using I-kappaB stabilization to determine the amount of metabolites of common CAM agents in urine (non-..
  70. Development of Dynamic Combinatorial Libraries for Cruzipain Inhibition
    Peter Wipf; Fiscal Year: 2009
    ..The biological assay will be carried out by Prof...
  71. HIV-related oral diseases among women in Harare
    John Greenspan; Fiscal Year: 2004
    ..Each participant would be seen at 6-month intervals over a 3-year period as part of the proposed study. ..
  72. ISOLATION OF A SECOND WILMS TUMOR SUPPRESSOR GENE
    Bernard Weissman; Fiscal Year: 2001
    ..In a complementary fashion, he has taken a functional approach by using a biological assay, tumor suppression, to map the locations of functional tumor suppressor genes via monochromosome transfer...
  73. Biomedical Polymers With Heparin-Binding End Groups
    Robert Ward; Fiscal Year: 2004
    ..The activity of the adsorbed heparin will first be determined by a chromogenic anti-Xa heparin assay. A biological assay that measures the amount of antithrombin III (ATIII) that binds to the heparinized surface will also be used ..
  74. Single and multi-component biguanide-based microbicides
    Mohamed Labib; Fiscal Year: 2007
    ..scaffold based on computational approaches coupled to iterative rounds of feedback from our biochemical and biological assay systems...
  75. Single and multi-component biguanide-based microbicides
    Mohamed Labib; Fiscal Year: 2006
    ..scaffold based on computational approaches coupled to iterative rounds of feedback from our biochemical and biological assay systems...
  76. A Nanofibrous Bioactive Prosthetic Sewing Cuff
    Matthew Phaneuf; Fiscal Year: 2009
    ..The associated health care costs from PVE alone are projected to be $50,000 per patient with a projected annual market in excess of $25-75 million. ..
  77. A New Class of Serine and Cysteine Protease Inhibitors
    CHRISTOPHER SETO; Fiscal Year: 2006
    ..Finally, the optimized inhibitors will be examined for their ability to inhibit dephosphorylation of the insulin receptor that is catalyzed by PTP1B. ..