spin trapping

Summary

Summary: A technique for detecting short-lived reactive FREE RADICALS in biological systems by providing a nitrone or nitrose compound for an addition reaction to occur which produces an ELECTRON SPIN RESONANCE SPECTROSCOPY-detectable aminoxyl radical. In spin trapping, the compound trapping the radical is called the spin trap and the addition product of the radical is identified as the spin adduct. (Free Rad Res Comm 1990;9(3-6):163)

Top Publications

  1. ncbi Identification of the myoglobin tyrosyl radical by immuno-spin trapping and its dimerization
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, PO Box 12233, MD F0 01, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:969-76. 2005
  2. ncbi Increased flux of free radicals in cells subjected to hyperthermia: detection by electron paramagnetic resonance spin trapping
    S W Flanagan
    Department of Exercise Science, The University of Iowa, Iowa City 52242, USA
    FEBS Lett 431:285-6. 1998
  3. ncbi Role of singlet oxygen in chloroplast to nucleus retrograde signaling in Chlamydomonas reinhardtii
    Beat B Fischer
    Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, Ueberlandstrasse 133, 8600, Dubendorf, Switzerland
    FEBS Lett 581:5555-60. 2007
  4. ncbi EPR spin trapping evaluation of ROS production in human fibroblasts exposed to cerium oxide nanoparticles: evidence for NADPH oxidase and mitochondrial stimulation
    Marcel Culcasi
    CNRS, Aix Marseille Universite, Institut de Chimie Radicalaire UMR 7273, Equipe Sondes Moléculaires en Biologie et Stress Oxydant, Marseille, France
    Chem Biol Interact 199:161-76. 2012
  5. pmc Obesity-induced tissue free radical generation: an in vivo immuno-spin trapping study
    Nicholas K H Khoo
    Department of Pharmacology and Chemical Biology, University of Pittsburgh, School of Medicine, Pittsburgh, PA 15213, USA
    Free Radic Biol Med 52:2312-9. 2012
  6. ncbi Acute methanol intoxication generates free radicals in rats: an ESR spin trapping investigation
    M B Kadiiska
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 28:1106-14. 2000
  7. ncbi Sensitive detection and localization of hydroxyl radical production in cucumber roots and Arabidopsis seedlings by spin trapping electron paramagnetic resonance spectroscopy
    Susannah Renew
    Institut fur Biologie II, Universitat Freiburg, Schänzlestr 1, 79104 Freiburg, Germany
    Plant J 44:342-7. 2005
  8. ncbi "Distant spin trapping": a method for expanding the availability of spin trapping measurements
    Nadeem Khan
    Department of Diagnostic Radiology, EPR Center for the Study of Viable Systems, 7785 Vail, Room 702, Dartmouth Medical School, Hanover, NH 03755, USA
    J Biochem Biophys Methods 62:125-30. 2005
  9. ncbi Neu2000, an NR2B-selective, moderate NMDA receptor antagonist and potent spin trapping molecule for stroke
    Sung Ig Cho
    Department of Neuroscience and Pharmacology, Ajou University, Suwon, Gyeonggido, South Korea
    Drug News Perspect 23:549-56. 2010
  10. ncbi Mechanism of hydrogen peroxide-induced Cu,Zn-superoxide dismutase-centered radical formation as explored by immuno-spin trapping: the role of copper- and carbonate radical anion-mediated oxidations
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T W Alexander Drive, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:201-14. 2005

Detail Information

Publications241 found, 100 shown here

  1. ncbi Identification of the myoglobin tyrosyl radical by immuno-spin trapping and its dimerization
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, PO Box 12233, MD F0 01, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:969-76. 2005
    5,5-Dimethyl-1-pyrroline N-oxide (DMPO) spin trapping in conjunction with antibodies specific for the DMPO nitrone epitope was used on hydrogen peroxide-treated sperm whale and horse heart myoglobins to determine the site of protein ..
  2. ncbi Increased flux of free radicals in cells subjected to hyperthermia: detection by electron paramagnetic resonance spin trapping
    S W Flanagan
    Department of Exercise Science, The University of Iowa, Iowa City 52242, USA
    FEBS Lett 431:285-6. 1998
    ..Using electron paramagnetic resonance (EPR) spin trapping, we sought direct evidence for free radical generation during hyperthermia in intact, functioning cells...
  3. ncbi Role of singlet oxygen in chloroplast to nucleus retrograde signaling in Chlamydomonas reinhardtii
    Beat B Fischer
    Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, Ueberlandstrasse 133, 8600, Dubendorf, Switzerland
    FEBS Lett 581:5555-60. 2007
    ..This indicates that singlet oxygen can stimulate a response farther from its production site than generally believed...
  4. ncbi EPR spin trapping evaluation of ROS production in human fibroblasts exposed to cerium oxide nanoparticles: evidence for NADPH oxidase and mitochondrial stimulation
    Marcel Culcasi
    CNRS, Aix Marseille Universite, Institut de Chimie Radicalaire UMR 7273, Equipe Sondes Moléculaires en Biologie et Stress Oxydant, Marseille, France
    Chem Biol Interact 199:161-76. 2012
    ..Electron paramagnetic resonance (EPR) spin trapping with the nitrone DEPMPO showed that pretreatment of the cells with the nanoparticles dose-dependently ..
  5. pmc Obesity-induced tissue free radical generation: an in vivo immuno-spin trapping study
    Nicholas K H Khoo
    Department of Pharmacology and Chemical Biology, University of Pittsburgh, School of Medicine, Pittsburgh, PA 15213, USA
    Free Radic Biol Med 52:2312-9. 2012
    ..However, immuno-spin trapping, a newly developed immunohistochemical technique for detection of free radical formation, is garnering ..
  6. ncbi Acute methanol intoxication generates free radicals in rats: an ESR spin trapping investigation
    M B Kadiiska
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 28:1106-14. 2000
    ..The spin trapping technique was used where a spin trapping agent, alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN), reacted ..
  7. ncbi Sensitive detection and localization of hydroxyl radical production in cucumber roots and Arabidopsis seedlings by spin trapping electron paramagnetic resonance spectroscopy
    Susannah Renew
    Institut fur Biologie II, Universitat Freiburg, Schänzlestr 1, 79104 Freiburg, Germany
    Plant J 44:342-7. 2005
    ..Here, it is shown that spin trapping electron paramagnetic resonance (EPR) spectroscopy allows the detection and quantitative estimation of OH* ..
  8. ncbi "Distant spin trapping": a method for expanding the availability of spin trapping measurements
    Nadeem Khan
    Department of Diagnostic Radiology, EPR Center for the Study of Viable Systems, 7785 Vail, Room 702, Dartmouth Medical School, Hanover, NH 03755, USA
    J Biochem Biophys Methods 62:125-30. 2005
    The technique of spin trapping is used to study a wide range of free radicals in various systems, including those generated in vitro and in vivo...
  9. ncbi Neu2000, an NR2B-selective, moderate NMDA receptor antagonist and potent spin trapping molecule for stroke
    Sung Ig Cho
    Department of Neuroscience and Pharmacology, Ajou University, Suwon, Gyeonggido, South Korea
    Drug News Perspect 23:549-56. 2010
    ..Neu2000 is an NR2B-selective, moderate NMDA receptor antagonist with potent cell-permeable, spin trapping antioxidant action even at nanomolar concentrations...
  10. ncbi Mechanism of hydrogen peroxide-induced Cu,Zn-superoxide dismutase-centered radical formation as explored by immuno-spin trapping: the role of copper- and carbonate radical anion-mediated oxidations
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T W Alexander Drive, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:201-14. 2005
    ..the biochemistry of H2O2-induced Cu,Zn-superoxide dismutase (SOD1)-centered radicals, detecting them by immuno-spin trapping. These radicals are involved in H2O2-induced structural and functional damage to SOD1, and their mechanism of ..
  11. ncbi Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species
    Honglian Shi
    College of Pharmacy, University of New Mexico, Albuquerque, NM, USA
    Arch Biochem Biophys 437:59-68. 2005
    Electron paramagnetic resonance with spin trapping is a useful technique to detect reactive oxygen species, such as superoxide radical anion (O2*-), a key species in many biological processes...
  12. ncbi Tryptophan-14 is the preferred site of DBNBS spin trapping in the self-peroxidation reaction of sperm whale metmyoglobin with a single equivalent of hydrogen peroxide
    Michael R Gunther
    Department of Biochemistry and Molecular Pharmacology, West Virginia University, Morgantown 26506, USA
    Chem Res Toxicol 16:652-60. 2003
    ....
  13. ncbi The free radical spin trapping agent phenylbutylnitrone reduces fetal brain DNA oxidation and postnatal cognitive deficits caused by in utero exposure to a non-structurally teratogenic dose of ethanol: a role for oxidative stress
    Lutfiya Miller
    Department of Pharmacology and Toxicology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada M5S 1A8
    Free Radic Biol Med 60:223-32. 2013
    ..gestational day 17 with 4g/kg EtOH or its saline vehicle, with or without pretreatment with the free radical spin trapping agent α-phenyl-N-tert-butylnitrone (PBN; 40mg/kg)...
  14. ncbi Inefficient spin trapping of superoxide in the presence of nitric-oxide: implications for studies on nitric-oxide synthase uncoupling
    Marc Pignitter
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Free Radic Biol Med 41:455-63. 2006
    ..Discrimination between these two compounds is usually achieved by spin trapping of superoxide...
  15. ncbi Oxidative stress studies of six TiO₂ and two CeO₂ nanomaterials: immuno-spin trapping results with DNA
    Kirk T Kitchin
    Integrated Systems Toxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, North Carolina 27711, USA
    Nanotoxicology 5:546-56. 2011
    ..trap agent 5,5-dimethyl-1-pyroline N-oxide (DMPO) to DNA as measured by the experimental technique of immuno-spin trapping. The eight nanomaterials differed considerably in their potency, slope, and active concentration...
  16. ncbi EPR spin trapping of oxygen radicals in plants: a methodological overview
    Goran Bacić
    Faculty of Physical Chemistry, University of Belgrade, Serbia and Montenegro
    Ann N Y Acad Sci 1048:230-43. 2005
    ..difficulties involved in detection of oxygen free radicals in plants and give a rationale for using the EPR spin trapping technique in such studies...
  17. ncbi Singlet oxygen scavenging activity of plastoquinol in photosystem II of higher plants: electron paramagnetic resonance spin-trapping study
    Deepak Kumar Yadav
    Laboratory of Biophysics, Department of Experimental Physics, Faculty of Science, Palacky University, Tr 17 listopadu 12, Olomouc, Czech Republic
    Biochim Biophys Acta 1797:1807-11. 2010
    ..These observations reveal that plastoquinol exogenously added to PQ-depleted PSII membranes serves as efficient scavenger of ¹O₂...
  18. ncbi Production of reactive oxygen species in chloride- and calcium-depleted photosystem II and their involvement in photoinhibition
    Andras Arato
    Institut fur Biologie II, Biochemie der Pflanzen, Universitat Freiburg, Schänzlestr 1, 79104 Freiburg, Germany
    Biochim Biophys Acta 1608:171-80. 2004
    ..reduction involving the prior formation of O(2)(-?), the production of reactive oxygen species was followed by spin trapping EPR spectroscopy. All samples investigated, i.e...
  19. ncbi Identification of free radicals in oxidized and glycoxidized phosphatidylethanolamines by spin trapping combined with tandem mass spectrometry
    Cláudia Simões
    Mass Spectrometry Center, QOPNA, Department of Chemistry, University of Aveiro, 3810 193 Aveiro, Portugal
    Rapid Commun Mass Spectrom 26:931-9. 2012
    ..Glycated PEs are more easily oxidized, enhancing oxidative stress. This study aims to evaluate the influence of glycation on the formation of intermediate radical species during oxidation of glycated PEs...
  20. ncbi Carotenoids as antioxidants: spin trapping EPR and optical study
    N E Polyakov
    Institute of Chemical Kinetics and Combustion, Novosibirsk, Russia
    Free Radic Biol Med 31:43-52. 2001
    ..It was shown that the spin trapping technique is more informative than optical methods for the experimental conditions under study...
  21. ncbi Using anti-5,5-dimethyl-1-pyrroline N-oxide (anti-DMPO) to detect protein radicals in time and space with immuno-spin trapping
    Ronald P Mason
    The National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 36:1214-23. 2004
    ..spin traps in conjunction with nitrone-antibody sensitivity and specificity greatly expands the utility of the spin trapping technique, which is no longer dependent on the quantum mechanical electron spin resonance (ESR)...
  22. ncbi Theoretical and experimental studies of the spin trapping of inorganic radicals by 5,5-dimethyl-1-pyrroline N-oxide (DMPO). 2. Carbonate radical anion
    Frederick A Villamena
    The Davis Heart and Lung Research Institute, The Division of Cardiovascular Medicine, Department of Internal Medicine, College of Medicine, The Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Phys Chem A 111:384-91. 2007
    ..Electron paramagnetic resonance spin trapping with the commonly used spin trap, DMPO, has been employed in the detection of CO(3)(.-)...
  23. ncbi A novel protocol to identify and quantify all spin trapped free radicals from in vitro/in vivo interaction of HO(.-) and DMSO: LC/ESR, LC/MS, and dual spin trapping combinations
    Steven Yue Qian
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:125-35. 2005
    ..CH(3)) that can be spin trapped and detected by electron spin resonance (ESR). This ESR spin trapping technique has been widely used in many biological systems to indicate in vivo HO(.-) formation...
  24. pmc Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS
    Suchandra Bhattacharjee
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 50:1536-45. 2011
    ..report here a qualitative measurement of DNA damage induced by a Cu(II)-H₂O₂ oxidizing system using immuno-spin trapping (IST) with electron paramagnetic resonance (EPR), MS, and MS/MS...
  25. ncbi Detection of reactive oxygen and nitrogen species by EPR spin trapping
    Frederick A Villamena
    Center for Biomedical EPR Spectroscopy and Imaging, and The Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, OH 43210, USA
    Antioxid Redox Signal 6:619-29. 2004
    Electron paramagnetic resonance spin trapping has become an indispensable tool for the specific detection of reactive oxygen free radicals in biological systems...
  26. ncbi Ethylene glycol generates free radical metabolites in rats: an ESR in vivo spin trapping investigation
    M B Kadiiska
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 13:1187-91. 2000
    ..The radical spin trapping technique was applied where the spin trapping agent alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN) ..
  27. pmc Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process
    Sandra E Gomez-Mejiba
    Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Free Radic Biol Med 46:853-65. 2009
    ..In this regard, we have developed and validated immuno-spin trapping to tag the redox process, tracing the oxidatively generated modification of biomolecules, in situ and in real ..
  28. pmc Immuno-spin trapping analyses of DNA radicals
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 TW Alexander Drive, Building 101, MD F0 02, Research Triangle Park, North Carolina 27709, USA
    Nat Protoc 2:512-22. 2007
    Immuno-spin trapping is a highly sensitive method for detecting DNA radicals in biological systems...
  29. ncbi Comparative DFT study of the spin trapping of methyl, mercapto, hydroperoxy, superoxide, and nitric oxide radicals by various substituted cyclic nitrones
    Frederick A Villamena
    The Davis Heart and Lung Research Institute, and the Division of Cardiovascular Medicine, Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Phys Chem A 109:1662-74. 2005
    The thermodynamics of the spin trapping of various cyclic nitrones with biologically relevant radicals such as methyl, mercapto, hydroperoxy, superoxide anion, and nitric oxide was investigated using computational methods...
  30. ncbi Identification of free radicals of glycerophosphatidylcholines containing omega-6 fatty acids using spin trapping coupled with tandem mass spectrometry
    A Reis
    Department of Chemistry, University of Aveiro, Aveiro 3810 193, Portugal
    Free Radic Res 41:432-43. 2007
    ..Free radical oxidation products were trapped by spin trapping with 5,5-dimethyl-1-pyrrolidine-N-oxide (DMPO) and identified by electrospray mass spectrometry (ES-MS)...
  31. ncbi Electron paramagnetic resonance spin trapping investigation into the kinetics of glutathione oxidation by the superoxide radical: re-evaluation of the rate constant
    Clare M Jones
    Gray Cancer Institute, Mount Vernon Hospital, Northwood, Middlesex HA6 2JR, UK
    Free Radic Biol Med 32:982-90. 2002
    ..Taking these factors into consideration, we estimate the second order rate constant for the oxidation of glutathione by superoxide to be approximately 200 M(-1) s(-1)...
  32. pmc Identifying the site of spin trapping in proteins by a combination of liquid chromatography, ELISA, and off-line tandem mass spectrometry
    Olivier M Lardinois
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 44:893-906. 2008
    An off-line mass spectrometry method that combines immuno-spin trapping and chromatographic procedures has been developed for selective detection of the nitrone spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) covalently attached to ..
  33. pmc Spin trapping investigation of peroxide- and isoniazid-induced radicals in Mycobacterium tuberculosis catalase-peroxidase
    Kalina Ranguelova
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, MD F0 01, 111 T W Alexander Drive, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 47:11377-85. 2008
    A new approach, the immuno-spin trapping assay, used a novel rabbit polyclonal anti-DMPO (5,5-dimethyl-1-pyrroline N-oxide) antiserum to detect protein radical-derived DMPO nitrone adducts in the hemoprotein Mycobacterium tuberculosis ..
  34. ncbi Formation of radicals from singlet oxygen produced during photoinhibition of isolated light-harvesting proteins of photosystem II
    Sara Rinalducci
    Department of Environmental Sciences, University of Tuscia, Largo dell Universita, 01100 Viterbo, Italy
    Biochim Biophys Acta 1608:63-73. 2004
    ..Thus, monomerization of antennae as well as their incorporation into the PSII complex seem to represent physiologically protected forms. A comparison is made of the photoinhibition mechanisms of different photosynthetic systems...
  35. ncbi Albumin oxidation to diverse radicals by the peroxidase activity of Cu,Zn-superoxide dismutase in the presence of bicarbonate or nitrite: diffusible radicals produce cysteinyl and solvent-exposed and -unexposed tyrosyl radicals
    Marcelo G Bonini
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, CP 26077, CEP 05513 970, Sao Paulo, SP, Brazil
    Biochemistry 43:344-51. 2004
    ..Overall, the results prove the diffusible and radical nature of the oxidants produced during the peroxidase activity of Cu,Zn-SOD in the presence of bicarbonate or nitrite...
  36. pmc New insights into the detection of sulfur trioxide anion radical by spin trapping: radical trapping versus nucleophilic addition
    Kalina Ranguelova
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 47:128-34. 2009
    ..that the radical adduct (DMPO/()SO(3)(-)) formation in biological systems is an artifact and not the result of spin trapping of sulfur trioxide anion radical (()SO(3)(-))...
  37. ncbi Immuno-spin trapping of DNA radicals
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T W Alexander Dr, Research Triangle Park, North Carolina 27709, USA
    Nat Methods 3:123-7. 2006
    The detection of DNA radicals by immuno-spin trapping (IST) is based on the trapping of radicals with 5,5-dimethyl-1-pyrroline N-oxide (DMPO), forming stable nitrone adducts that are then detected using an anti-DMPO serum...
  38. ncbi Photoexcitation of aqueous suspensions of titanium dioxide nanoparticles: an electron spin resonance spin trapping study of potentially oxidative reactions
    Nicholas J F Dodd
    Ecotoxicology Research and Innovation Centre, School of Biomedical and Biological Sciences, University of Plymouth, Drake Circus, Plymouth, UK
    Photochem Photobiol 87:632-40. 2011
    ..Hydroxyl radicals formed at the surface of the TiO(2) readily react with substrates containing an abstractable hydrogen to produce secondary radicals that, in biological systems, could lead to cell damage...
  39. pmc Protein Radical Formation Resulting from Eosinophil Peroxidase-catalyzed Oxidation of Sulfite
    Kalina Ranguelova
    Laboratory of Pharmacology, NIEHS, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Biol Chem 285:24195-205. 2010
    ..We used immuno-spin trapping and confocal microscopy to study protein oxidation by EPO/H(2)O(2) in the presence of bisulfite in a pure ..
  40. ncbi Spin trapping of superoxide radicals and peroxynitrite by 1-hydroxy-3-carboxy-pyrrolidine and 1-hydroxy-2,2,6, 6-tetramethyl-4-oxo-piperidine and the stability of corresponding nitroxyl radicals towards biological reductants
    S Dikalov
    Institute of Chemical Kinetics and Combustion, Novosibirsk, Russia
    Biochem Biophys Res Commun 231:701-4. 1997
    ..2mM of spin trap CP-H or 1-hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine (TEMPONE-H) provide almost the same spin trapping efficacy. In contrast to TEMPONE-H the reaction of CP-H with peroxynitrite was inhibited by 20 mM DMSO...
  41. ncbi Protein radical formation on thyroid peroxidase during turnover as detected by immuno-spin trapping
    Marilyn Ehrenshaft
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 41:422-30. 2006
    ..Evidence that correlates iodine deficiencies with development of thyroid autoimmune disorders supports this conjecture...
  42. ncbi Spin trapping of polyunsaturated fatty acid-derived peroxyl radicals: reassignment to alkoxyl radical adducts
    S I Dikalov
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 30:187-97. 2001
    ..of the detection of PUFA peroxyl radical adducts; however, it has recently been reported that attempted spin trapping of organic peroxyl radicals at room temperature formed only alkoxyl radical adducts in detectable amounts...
  43. ncbi Production of free radicals measured by spin trapping during operations for stenosis of the carotid artery
    J Holm
    Department of Surgery, Sahlgrenska University Hospital, , Sweden
    Eur J Surg 167:4-9. 2001
    ..CONCLUSIONS: The technique used seems to offer the possibility to find and study methods to reduce free radical production during this kind of operation...
  44. ncbi Electron transfer between a tyrosyl radical and a cysteine residue in hemoproteins: spin trapping analysis
    Suchandra Bhattacharjee
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Am Chem Soc 129:13493-501. 2007
    ..DMPO) and myoglobin (Mb)/hydrogen peroxide/DMPO, using a combination of techniques including ESR, immuno-spin trapping (IST), and ESI/MS...
  45. ncbi High static pressure alters spin trapping rates in solution. Dependence on the structure of nitrone spin traps
    Yoshimi Sueishi
    Department of Chemistry, Faculty of Science, Okayama University, 3 1 1 Tsushima naka, Okayama 700 8530, Japan
    Org Biomol Chem 4:896-901. 2006
    Using a competitive spin trapping method, relative spin trapping rates were quantified for various short-lived radicals (methyl, ethyl, and phenyl radicals)...
  46. ncbi Photo-oxidation of diglycine in confined media Relaxation of longitudinal magnetization in spin correlated radical pairs
    Valery F Tarasov
    Semenov Institute of Chemical Physics RAS, Moscow 199999, Russia
    Spectrochim Acta A Mol Biomol Spectrosc 63:776-83. 2006
    ..This explains time and micelle size dependence of the relative contribution of RPM into TREPR spectra. It seems reasonable to suggest that the creation of the initial spin state populations is partially adiabatic...
  47. ncbi The reaction rate of edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186)) with hydroxyl radical
    Shinji Abe
    Department of Pharmacology, The University of Tokushima School of Medicine, Tokushima, Japan
    Chem Pharm Bull (Tokyo) 52:186-91. 2004
    ..The possible mechanisms for oxidation of edaravone were investigated from these findings...
  48. ncbi Formation of superoxide anion during ferrous ion-induced decomposition of linoleic acid hydroperoxide under aerobic conditions
    Yasuhiro Kambayashi
    Department of Photon and Free Radical Research, Japan Immunoresearch Laboratories, 351 1 Nishiyokote cho, Takasaki 370 0021
    J Biochem 134:903-9. 2003
    ..formation of oxygen radicals during ferrous ion-induced decomposition of linoleic acid hydroperoxide using the spin trapping and chemiluminescence methods. The formation of the superoxide anion (O2*-) was verified in the present study...
  49. ncbi In vivo identification of aflatoxin-induced free radicals in rat bile
    Rheal A Towner
    Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
    Free Radic Biol Med 35:1330-40. 2003
    ..spin resonance (ESR) spectroscopy] free radicals in vivo in rat bile following AFB1 metabolism using the spin trapping [alpha-(4-pyridyl-1-oxide)-N-tert-butyl nitrone (4-POBN)] technique...
  50. ncbi Reduction of nitroxides and radioprotective ability in glioblastoma cells
    Takashi Moritake
    Department of Neurosurgery, Institute of Clinical Medicine, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki 305 8575, Japan
    Brain Tumor Pathol 20:1-5. 2003
    ..It was indicated that the resistance of glioblastoma cells to ionizing radiation could be closely related to their ability to scavenge radical species generated by ionizing radiation...
  51. ncbi Spin trapping of superoxide, alkyl- and lipid-derived radicals with derivatives of the spin trap EPPN
    Klaus Stolze
    Basic Pharmacology and Toxicology, Institute of Applied Botany, University of Veterinary Medicine Vienna, Veterinarplatz 1, A 1210 Vienna, Austria
    Biochem Pharmacol 66:1717-26. 2003
    ....
  52. ncbi Vascular effects of singlet oxygen (1O2) generated by photo-excitation on adrenergic neurotransmission in isolated rabbit mesenteric vein
    Fumihiko Yoshino
    Department of Pharmacology and ESR Laboratories, Kanagawa Dental College, Kanagawa, Yokosuka, Japan
    Redox Rep 7:266-70. 2002
    ..These results suggest that 1O2 may induce NA-mediated vasoconstriction at the postjunctional site, and may be associated with Ca(2+)-independent NA release from the prejunctional site of adrenergic neurotransmission...
  53. ncbi Inhibition of superoxide generation from fMLP-stimulated leukocytes by high concentrations of nitric oxide or peroxynitrite: characterization by electron spin resonance spectroscopy
    Masaichi Chang Il Lee
    Department of Pharmacology and ESR Laboratories, Kanagawa Dental College, Kanagawa, Yokosuka, Japan
    Redox Rep 7:271-5. 2002
    ..fMLP)-stimulated polymorphonuclear leukocytes (PMNs) by using electron spin resonance (ESR) and spin trapping with 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)...
  54. ncbi Antioxidant activity of Sempervivum tectorum and its components
    Marjeta Sentjurc
    EPR Center, J Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    J Agric Food Chem 51:2766-71. 2003
    ..Parallel assays that measured thiobarbituric acid related substances confirm the antioxidant effects of KA and KG and explain the results of spin trapping experiments complicated by low pH's.
  55. ncbi Spin traps: in vitro toxicity and stability of radical adducts
    Nadeem Khan
    Department of Diagnostic Radiology, EPR Center, Dartmouth Medical School, Hanover, NH 03755, USA
    Free Radic Biol Med 34:1473-81. 2003
    ..It then should be feasible to select the spin trap(s) best suited for the proposed study...
  56. ncbi UV-induced free radicals in the skin detected by ESR spectroscopy and imaging using nitroxides
    T Herrling
    Privatinstitut Galenus GmbH, Volmerstrasse 7A, D 12489 Berlin, Germany
    Free Radic Biol Med 35:59-67. 2003
    ..This reduction was shown to be caused mainly by UVA radiation (320-400 nm)...
  57. ncbi Scavenger capacity of follicular fluid, decidua and culture medium with regard to assisted reproduction: an in vitro study using electron paramagnetic resonance spectroscopy
    Attila Torok
    Department of Obstetrics and Gynecology, County Hospital, Pecs, Hungary
    Gynecol Obstet Invest 55:178-82. 2003
    ..Presumably, the follicular fluid and endometrium samples contain active factors which function as radical scavengers. The findings suggest that appropriate augmentative antioxidative therapy might be advised for IVF patients...
  58. ncbi Photodynamic effects of two hydroxyanthraquinones
    M Rajendran
    Department of Chemistry, NMSSVN College, Madurai 625 019, Tamil Nadu, India
    Biochim Biophys Acta 1622:65-72. 2003
    ..Both optical and ESR measurements indicate that O2-* (Type I) and 1O2 (Type II) paths are involved in CYN and CYNM photodynamic action...
  59. ncbi Esters of 5-carboxyl-5-methyl-1-pyrroline N-oxide: a family of spin traps for superoxide
    Pei Tsai
    Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, Maryland 21201, USA
    J Org Chem 68:7811-7. 2003
    ..At acidic pH, the rate constant for spin trapping O2*- was 3-fold greater than that at physiological pH...
  60. ncbi Spin-trapping of oxygen free radicals in chemical and biological systems: new traps, radicals and possibilities
    Goran Bacić
    Faculty for Physical Chemistry, Studentski trg 12 16, Belgrade 11000, Serbia
    Spectrochim Acta A Mol Biomol Spectrosc 69:1354-66. 2008
    ..As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (*H) atom...
  61. ncbi Superoxide radical anion adduct of 5,5-dimethyl-1-pyrroline n-oxide (DMPO). 1. The thermodynamics of formation and its acidity
    Frederick A Villamena
    Center for Biomedical EPR Spectroscopy and Imaging, The Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Phys Chem A 109:6083-8. 2005
    ..Under acidic conditions, the addition of *O2H to DMPO is predicted to be more exoergic than the addition of O2*- and is consistent with available experimental kinetic data...
  62. ncbi Development of a new EPR spin trap, DOD-8C (N-[4-dodecyloxy-2-(7'-carboxyhept-1'-yloxy)benzylidene]-N-tert-butylamine N-oxide), for the trapping of lipid radicals at a predetermined depth within biological membranes
    Alison Hay
    Department of Chemistry, University of Glasgow, Glasgow, G12 8QQ, UK
    Arch Biochem Biophys 435:336-46. 2005
    ..This molecule represents the first of a new generation of spin traps which, through modification, can be used to position the radical-trapping nitrone moiety at chosen depths within biological membranes...
  63. ncbi Scavenging of reactive oxygen species by the plant phenols genistein and oleuropein
    Irena Kruk
    Institute of Physics, Technical University of Szczecin, Al Piastow 48 49, 70 311 Szczecin, Poland
    Luminescence 20:81-9. 2005
    ..It was also demonstrated that genistein had a weaker DPPH radical scavenging activity than oleuropein. Our results confirm good scavenging activity towards O(2).(-), HO. and ROO. and the antioxidant effect of genistein and oleuropein...
  64. ncbi Very stable superoxide radical adducts of 5-ethoxycarbonyl- 3,5-dimethyl-pyrroline N-oxide (3,5-EDPO) and its derivatives
    Klaus Stolze
    Research Institute of Biochemical Pharmacology and Toxicology, University of Veterinary Medicine Vienna, Veterinarplatz 1, A 1210 Vienna, Austria
    Biochem Pharmacol 69:1351-61. 2005
    ..12-55 min). In addition, various other spin adducts obtained from oxygen-centered as well as carbon-centered radicals (e.g. derived from methanol or linoleic acid hydroperoxide) were also detected...
  65. ncbi EPR spin trapping of a radical intermediate in the urate oxidase reaction
    E Busi
    Dipartimento di Chimica, Scienze Endocrino Metaboliche e Biochimica, Universita di Siena, Siena, Italy
    Nucleosides Nucleotides Nucleic Acids 23:1131-4. 2004
    ..In this study EPR spectroscopy by spin trapping of radical intermediates has been used in order to demonstrate the eventual presence of radical transient ..
  66. ncbi 2-substituted-3H-indol-3-one-1-oxides: preparation and radical trapping properties
    Jeremie Boyer
    Laboratoire Pharmacochimie des Substances Naturelles et Pharmacophores Redox, UMR IRD UPS 152, Faculte de Pharmacie, Universite Paul Sabatier, 31062 Toulouse 4, France
    Free Radic Res 38:459-71. 2004
    ..b>Spin trapping and electron paramagnetic resonance experiments demonstrate the ability of these indolone-1-oxides to trap ..
  67. ncbi Novel sparse component analysis approach to free radical EPR spectra decomposition
    Chunqi Chang
    Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong
    J Magn Reson 175:242-55. 2005
    ..This method can also be used in other similar applications of quantitative spectroscopy analysis...
  68. ncbi Hydroxyl radical generation caused by the reaction of singlet oxygen with a spin trap, DMPO, increases significantly in the presence of biological reductants
    Chiho Nishizawa
    Redox Regulation Research Group, National Institute of Radiological Sciences, 4 9 1, Anagawa, Inage Ku, Chiba 263 8555, Japan
    Free Radic Res 38:385-92. 2004
    ..of singlet oxygen (1O2) with spin traps, although this knowledge is necessary in electron spin resonance (ESR)/spin trapping. In the present study, we examined the effect of various reductants usually present in biological samples on ..
  69. ncbi Characterization of the high-resolution ESR spectra of superoxide radical adducts of 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Analysis of conformational exchange
    Sergey Dikalov
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Res 39:825-36. 2005
    ..It is clear that to correctly interpret DEPMPO spin trapping of superoxide radicals, one must carefully consider formation of secondary radical adducts.
  70. ncbi Free radical trapping properties of several ethyl-substituted derivatives of 5-ethoxycarbonyl-5-methyl-1-pyrroline N-oxide (EMPO)
    Klaus Stolze
    Molecular Pharmacology and Toxicology Unit, Department of Natural Science, University of Veterinary Medicine Vienna, Veterinarplatz 1, A 1210 Vienna, Austria
    Bioorg Med Chem 15:2827-36. 2007
    The spin trapping behavior of several ethyl-substituted EMPO derivatives, cis- and trans-5-ethoxycarbonyl-3-ethyl-5-methyl-pyrroline N-oxide (3,5-EEMPO), 5-ethoxycarbonyl-4-ethyl-5-methyl-pyrroline N-oxide (4,5-EEMPO), cis- and trans-5-..
  71. ncbi Early specific free radical-related cytotoxicity of gas phase cigarette smoke and its paradoxical temporary inhibition by tar: An electron paramagnetic resonance study with the spin trap DEPMPO
    Marcel Culcasi
    Laboratoire Structure et Réactivité des Espèces Paramagnétiques, Sondes Moleculaires en Biologie, CNRS UMR 6517 Universités d Aix Marseille I and III, Marseille, France
    Chem Biol Interact 164:215-31. 2006
    Electron paramagnetic resonance (EPR) spin trapping studies demonstrated aqueous tar particulate matter (TPM) and gas phase cigarette smoke (GPCS) to behave as different sources of free radicals in cigarette smoke (CS) but their ..
  72. ncbi Photodynamic action of C-phycocyanins obtained from marine and fresh water cyanobacterial cultures: a comparative study using EPR spin trapping technique
    Bibbin Tom Paul
    Centre for Potential in Genomic Science, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India
    Free Radic Res 40:821-5. 2006
    ..Efficiency of 1O2 generation is of the order LY > PHR> SPI. The yield of reactive oxygen species (ROS) generation is found to be 1O2>O2(-) indicating type II mechanism to be the prominent pathway for photosensitation by phycocyanins...
  73. ncbi Spin trapping of glutathiyl and protein radicals produced from nitric oxide-derived oxidants
    Ohara Augusto
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, CP 26077, CEP 05599 970, Sao Paulo, SP, Brazil
    Free Radic Biol Med 36:1224-32. 2004
    ..New approaches based on spin trapping coupled with other methodologies are under development/testing but so far they have been applied mainly to the ..
  74. ncbi Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin
    Th Herrling
    FOM Institute, University of Applied Science and Technology TFH Berlin, Berlin, Germany
    Spectrochim Acta A Mol Biomol Spectrosc 63:840-5. 2006
    ..Regarding the amount of generated free radicals/ROS in skin as an biophysical endpoint the RSF characterizes both the protection against UVB and UVA radiation...
  75. ncbi Sulfite-radical anions in isolated soy proteins
    Q Lei
    Dept of Animal and Food Sciences, Univ of Kentucky, 408 W P Garrigus Building, Lexington, KY 40546 0215, USA
    J Food Sci 72:C300-6. 2007
    ..radicals generated in the sulfite, manganese, and oxygen reaction mixture is inhibited by the free radical spin trapping agent 5,5-dimethyl-1-pyrroline N-oxide...
  76. ncbi Electron paramagnetic resonance investigation of in vivo free radical formation and oxidative stress induced by 2,4-dichlorophenol in the freshwater fish Carassius auratus
    Yi Luo
    State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210093, China
    Environ Toxicol Chem 24:2145-53. 2005
    ....
  77. ncbi Scavenging of reactive oxygen species by some nonsteroidal anti-inflammatory drugs and fenofibrate
    Aleksandra Kładna
    Department of Medical History and Ethics, Pomeranian Medical Academy, Szczecin, Poland
    Biopolymers 82:99-105. 2006
    ..Hence, these results indicate that the studied drugs show broad ROS scavenging property and, as a consequence, might decrease tissue damage due to the ROS and thus to contribute to anti-inflammatory therapy...
  78. ncbi Scavenging effects of phenolic compounds on reactive oxygen species
    Hassan Y Aboul-Enein
    Pharmaceutical and Medicinal Chemistry Department, The Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo 12311, Egypt
    Biopolymers 86:222-30. 2007
    ..We have employed chemiluminescence, electron spin resonance spin trapping, and spectrophotometry techniques to examine an antioxidative property...
  79. ncbi Molecular motion of spin labeled side chains in alpha-helices: analysis by variation of side chain structure
    L Columbus
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:3828-46. 2001
    ..The saturated 4-substituted pyrrolidine nitroxides are shown to be a suitable template for novel "functionalized" side chains designed to mimic salient features of the native side chains they replace...
  80. ncbi Spin adducts of superoxide, alkoxyl, and lipid-derived radicals with EMPO and its derivatives
    Klaus Stolze
    Institute of Pharmacology and Toxicology, Veterinary University of Vienna, Austria
    Biol Chem 383:813-20. 2002
    ..Thus, within this series EMPO is the best spin trap for the detection of superoxide; OcMPO, on the other hand, is most suitable for the detection of lipid alkoxyl radicals...
  81. ncbi Detection of free radicals in ischemic and reperfused canine gracilis muscle flaps by use of spin-trapping electron paramagnetic resonance spectroscopy
    B A Brisson
    Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Canada
    Am J Vet Res 62:384-8. 2001
    ..Spin-trapping EPR spectroscopy may be useful for the study of antioxidants and free radical scavengers in attenuating ischemia and reperfusion-mediated skeletal muscle damage...
  82. ncbi Enhanced intraarticular free radical reactions in adjuvant arthritis rats
    Ken ichi Yamada
    Graduate School of Pharmaceutical Sciences, Kyushu University, Department of Bio function Science, Maidashi, Fukuoka, Japan
    Free Radic Res 40:455-60. 2006
    ..This technique would be useful tool to determine the location of the enhanced free radical reactions and evaluate the activity of antioxidant medicine with non-invasive real-time measurement...
  83. ncbi Detection and removal of contaminating hydroxylamines from the spin trap DEPMPO, and re-evaluation of its use to indicate nitrone radical cation formation and S(N)1 reactions
    Simon K Jackson
    Department of Medical Microbiology, University of Wales College of Medicine, Cardiff, Wales, UK
    Free Radic Biol Med 32:228-32. 2002
    ..As a result, techniques for the detection and elimination of such hydroxylamine impurities from DEPMPO solutions were developed and are described; these should prove to be of general use in EPR spin trapping experiments.
  84. ncbi Spin trapping agent, phenyl N-tert-butylnitrone, reduces nitric oxide production in the rat brain during experimental meningitis
    H Endoh
    Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry, 2-2-1 Matsuei, Yamagata 990-2473, Japan
    Free Radic Res 35:583-91. 2001
    Phenyl N-tert-butylnitrone (PBN) is a spin trapping agent previously shown to exert a neuroprotective effect in infant rat brain during bacterial meningitis...
  85. ncbi Superoxide radical anion adduct of 5,5-dimethyl-1-pyrroline N-oxide (DMPO). 2. The thermodynamics of decay and EPR spectral properties
    Frederick A Villamena
    Center for Biomedical EPR Spectroscopy and Imaging, The Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Phys Chem A 109:6089-98. 2005
    ..Some pathways for the bimolecular decomposition of DMPO-O2H and DMPO-OH have also been computed...
  86. ncbi A novel method of measuring hydroxyl radical-scavenging activity of antioxidants using gamma-irradiation
    H Yoshioka
    Radiochemistry Research Laboratory, Faculty of Science, Shizuoka University, 836 Ohya, Shizuoka-shi 422-8529, Japan
    Free Radic Res 35:265-71. 2001
    A new method using ESR spin trapping was proposed for measuring the scavenging activity of antioxidants for the hydroxyl (OH) radical...
  87. ncbi Evidence for isoniazid-dependent free radical generation catalyzed by Mycobacterium tuberculosis KatG and the isoniazid-resistant mutant KatG(S315T)
    N L Wengenack
    Department of Biochemistry and Molecular Biology and Section of Hematology Research, Mayo Clinic, Rochester, Minnesota 55905, USA
    Biochemistry 40:8990-6. 2001
    ..In this work, EPR spin trapping experiments detected isoniazid-derived radicals generated during KatG-mediated oxidation via the peroxidase ..
  88. ncbi New evidence that the Alzheimer beta-amyloid peptide does not spontaneously form free radicals: an ESR study using a series of spin-traps
    S Turnbull
    Department of Biological Sciences, University of Lancaster, Lancaster, UK
    Free Radic Biol Med 30:1154-62. 2001
    ....
  89. ncbi NMR spin trapping: detection of free radical reactions with a new fluorinated DMPO analog
    V V Khramtsov
    Dorothy M. Davis Heart and Lung Research Institute, Department of Internal Medicine, Pulmonary and Critical Care Medicine, Ohio State University, Columbus, OH, USA
    Free Radic Biol Med 30:1099-107. 2001
    Electron spin resonance (ESR) and nuclear magnetic resonance (NMR) spin trapping were used for detection of free radical reactions utilizing a new fluorinated analog of DMPO, 4-hydroxy-5,5-dimethyl-2-trifluoromethylpyrroline-1-oxide (..
  90. ncbi Interleukin-10-mediated inhibition of free radical generation in macrophages
    S Dokka
    Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506, USA
    Am J Physiol Lung Cell Mol Physiol 280:L1196-202. 2001
    ..OH radicals in response to LPS stimulation. These results demonstrate, for the first time, direct evidence for the role of IL-10 in ROS-dependent NF-kappaB activation...
  91. ncbi Ultraviolet irradiation of titanium dioxide in aqueous dispersion generates singlet oxygen
    R Konaka
    Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, Abeno, Osaka, Japan
    Redox Rep 6:319-25. 2001
    ....
  92. ncbi Spin trapping study of reactive oxygen species formation during bopindolol peroxidation
    Irena Kruk
    Institute of Physics, Technical University of Szczecin, Al Piastow 48 49, 70 310 Szczecin, Poland
    Biopolymers 65:89-94. 2002
    ..The data presented here give new evidence for generation of the reactive oxygen species during peroxidation of bopindolol...
  93. ncbi Applicability of new spin trap agent, 2-diphenylphosphinoyl-2-methyl-3,4-dihydro-2H-pyrrole N-oxide, in biological system
    Tomohiro Karakawa
    Division of Pharmacology and Therapeutics, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 5 1 Oe honmachi, Kumamoto 862 0973, Japan
    Biochem Biophys Res Commun 370:93-7. 2008
    ..was significantly higher than that of DMPO (k(DMPO)=13.95M(-1)s(-1), k(DPhPMPO)=42.4M(-1)s(-1)). These results indicated that DPhPMPO is a potentially good candidate for trapping O2.- in a biological system...
  94. ncbi [Effects of reactive species of oxygen and nitrogen on iron ion release from ferritin and synthesis of dinitrosyl iron complexes]
    K B Shumaev
    Russian Cardiological Research Center, Ministry of Healht of Russian Federation, Tret ya Cherepkovskaya ul 15A, Moscow, 121552 Russia
    Biofizika 48:5-10. 2003
    ..It was proposed that the equilibrium between S-nitrosoglutathione, DNIC, "free Fe" ions and ferritin may determine the balance between prooxidant and antioxidant processes in living organisms...
  95. ncbi Generation of free radicals and/or active oxygen by light or laser irradiation of hydrogen peroxide or sodium hypochlorite
    Midori Kashima-Tanaka
    Department of Endodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan
    J Endod 29:141-3. 2003
    ..On the other hand, the amounts of DMPO-X generated from NaClO after irradiation were in the order: plasma lamp > Yellow He-Ne laser > halogen lamp > He-Ne laser...
  96. pmc LC/ESR/MS study of spin trapped carbon-centred radicals formed from in vitro lipoxygenase-catalysed peroxidation of gamma-linolenic acid
    Qingfeng Yu
    Department of Pharmaceutical Sciences, College of Pharmacy, Nursing, and Allied Sciences, North Dakota State University, Fargo, ND 58105, USA
    Free Radic Res 42:442-55. 2008
    ..The results provided a good starting point for ongoing research in defining the possible biological effects of radicals generated from GLA peroxidation...
  97. ncbi Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals
    H Zhao
    Biophysics Research Institute and Free Radical Research Center, The Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 31:599-606. 2001
    ..These favorable chemical and spectroscopic features make BMPO ideal for the detection of superoxide anions, hydroxyl and thiyl radicals in biochemical oxidation and reduction...
  98. ncbi Sumatriptan scavenges superoxide, hydroxyl, and nitric oxide radicals: in vitro electron spin resonance study
    Yukio Ikeda
    Department of Neurosurgery, Showa University School of Medicine, Tokyo, Japan
    Headache 42:888-92. 2002
    ..Oxygen free radicals have been implicated in the genesis of many pathological processes, including migraine. Sumatriptan succinate is known to be a very effective drug for acute relief of migraine attack...
  99. ncbi Detection and characterization of cyclic hydroxylamine adducts by mass spectrometry
    Ana Reis
    Mass Spectrometry Group, Department of Chemistry, University of Aveiro, Aveiro, Portugal
    Free Radic Res 42:481-91. 2008
    ..The biomolecule adducts identified by MS are ESR silent, evidencing the importance of MS detection...
  100. ncbi Biochemical reactivity of melatonin with reactive oxygen and nitrogen species: a review of the evidence
    R J Reiter
    Department of Cellular and Structural Biology, The University of Texas Health Science Center, San Antonio 78299 3900, USA
    Cell Biochem Biophys 34:237-56. 2001
    ....
  101. ncbi Depletion of energy metabolites following acetylcholinesterase inhibitor-induced status epilepticus: protection by antioxidants
    R C Gupta
    Toxicology Department, Breathitt Veterinary Center, Murray State University, Hopkinsville, KY, USA
    Neurotoxicology 22:271-82. 2001
    ..In the present study, we examined whether the spin trapping agent N-tertbutyl-alpha-phenylnitrone (PBN) and the antioxidant vitamin E (DL-alpha-tocopherol) protect levels ..

Research Grants62

  1. Prevention of UV-induced carcinogenesis by cyanidin-3-glucoside
    Xianglin Shi; Fiscal Year: 2013
    ..In vitro and in vivo investigation of antioxidant properties of C3G. Electron spin resonance (ESR) spin trapping will be used to determine the reaction rates of C3G toward hydroxyl (7OH) and superoxide (O27-) radicals, ..
  2. Transplantation of Reduced-Size Fatty Livers
    Zhi Zhong; Fiscal Year: 2010
    ....
  3. Quercitrin functions as an antioxidant and protects UV-induced carcinogenesis
    Zhuo Zhang; Fiscal Year: 2012
    ..free radical generation from UV- irradiated skin of living animal using in vivo electron spin resonance (ESR) spin trapping and identify the radicals generated...
  4. Radical Intermediates of Nitric Oxide Synthase &Myocardial Ischemia Reperfusion
    Ah Lim Tsai; Fiscal Year: 2013
    ..The regulation of these radical intermediates by substrate, cofactors and thiol also are studied, both in vitro and ex vivo, in order to elucidate the underlying disease mechanism of myocardial ischemia and reperfusion injury. ..
  5. ROS Mechanisms in BAV Aortopathy
    THOMAS GILLETTE GLEASON; Fiscal Year: 2013
    ..ROS will be evaluated using the investigators'expertise in electron paramagnetic resonance spin trapping and confocal microscopy of fluorescence-based ROS probes...
  6. OXIDATIVE STRESS AND ANTIOXIDANTS IN IRON OVERLOAD
    William Bernard Weglicki; Fiscal Year: 2010
    ..Various sophisticated biophysical (ESR spin trapping), immunochemical and molecular biology techniques (real-time PCR) will be used to assess the sequence of ..
  7. Free Radicals and Redox Signaling Triggered by Lipopolysccharide in Macrophages
    Dario C Ramirez; Fiscal Year: 2010
    ..The specific aims are designed to test our hypothesis using immuno-spin trapping (1ST) with the nitrone spin trap 5,5,-dimethyM-pyrroline A/-oxide (DMPO) and to improve our methods of ..
  8. MEASUREMENT OF FREE RADICAL GENERATION IN THE HEART
    JAY LOUIS ZWEIER; Fiscal Year: 2010
    ..Direct and spin trapping EPR studies will be performed to measure, quantitate, and characterize oxygen radical and NO generation in ..
  9. EPR 2010
    Balaraman Kalyanaraman; Fiscal Year: 2010
    ..be a joint conference of the 14th In Vivo EPR Spectroscopy and Imaging meeting and the 11th International EPR Spin Trapping/Spin Labeling meeting. It will be held May 2-6, 2010 at the Condado Plaza Hotel, San Juan, Puerto Rico...
  10. EPR Detection of Free Radicals in Vascular Disease: Bruker EMXPlus Spectrometer
    ERIC EUGENE KELLEY; Fiscal Year: 2013
    ..For less stable free radical species, EPR spin trapping techniques permit detection and identification in a multitude of in vitro and in vivo systems...
  11. Detection of Intracellular Oxygen Species
    Sameh S Ali; Fiscal Year: 2011
    ..decreased ROS levels? and iii) What are the best therapeutic strategies to ameliorate or reverse changes in ROS without compromising their signaling functions? ..
  12. Microxymetry Techniques for Cardiomocyte Respiration
    Govindasamy Ilangovan; Fiscal Year: 2005
    ..The present approach combines the EPR oxymetry and spin trapping into one, to get both oxygen concentration and free radicals concentration in one step...
  13. OXYGEN RADICALS AND CARDIAC ADAPTATIONS TO ISCHEMIA
    Roberto Bolli; Fiscal Year: 1999
    ..of oxygen-derived free radicals will be directly investigated by in vivo detection and quantification with spin trapping. A broad multidisciplinary approach will be used that will combine diverse techniques (free radical chemistry ..
  14. Mechanisms of PCP genotoxicity & antioxidant modulation
    BENZHAN ZHU; Fiscal Year: 2002
    ....
  15. FREE RADICALS, PROTEIN AGGREGATES & PARKINSON'S DISEASE
    GARY PIELAK; Fiscal Year: 2001
    ..Aim 4 is to show that crosslinking accelerates alpha-synuclein aggregation. Spin trapping will be used to stabilize the radicals. Mass spectrometry will be used to count the radical sites...
  16. MONOCYTE/MACROPHAGE FREE RADICAL BIOLOGY ASSESSED BY EPR
    Bradley Britigan; Fiscal Year: 1993
    ..I helped develop such a system which utilizes spin trapping techniques in conjunction with electron paramagnetic resonance spectrometry...
  17. FAMILIAL ALS--MECHANISM OF INITIATION
    Dale Bredesen; Fiscal Year: 1999
    ..3) peroxynitrite and 4) dioxygen and substrates with 3 substrates for the inactivation reaction (DMPO spin trapping and oxidation of ferrocytochrome or ABTS)...
  18. CADMIUM TOXICOLOGY
    Curtis Klaassen; Fiscal Year: 2000
    ..tissue analysis (antioxidant components vs tissue Cd content), and (e) electron paramagnetic resonance (ESR)-spin trapping to detect free radical metabolites generated in vivo during chronic Cd exposure...
  19. ROLE OF NITRIC OXIDE IN SUPEROXIDE-MEDIATED PATHOLOGY
    JOSEPH BECKMAN; Fiscal Year: 1993
    ..first few minutes of reperfusion of ischemic heart which corresponds with free radical production measured by spin trapping. We found that -OONO reacts with Cu, Zn SOD to form a stable complex, allowing us to grow crystals and to ..
  20. MECHANISMS OF MICROWAVE DAMAGE IN OCULAR TISSUE
    David Lange; Fiscal Year: 1992
    ....
  21. SUBSTANCE P-MEDIATED CARDIOVASCULAR INFLAMMATION
    William Weglicki; Fiscal Year: 2007
    ..biochemical (tissue glutathione and antioxidant status, plasma isoprotane level) and biophysical (ESR-spin trapping of free radicals and NO in isolated perfused heads) approaches will also be used...
  22. Regulation of Mitochondrial Nitric-oxide Synthase
    Cecilia Giulivi; Fiscal Year: 2003
    ..this molecule was demonstrated by the oxidation of oxymyoglobin, electron spin resonance spectroscopy with the spin trapping technique, and L-citrulline production...
  23. MECHANISMS OF OXIDATIVE DAMAGE IN BRAIN
    ROBERT FLOYD; Fiscal Year: 1993
    ..D) 31P-NMR in vivo spectroscopy will be used to standardize the extent of cortical ischemia for subsequent determination of oxidative damage to brain tissue...
  24. BIOMEDICAL RESEARCH SUPPORT
    PAUL HOCHSTEIN; Fiscal Year: 1991
    Research activity involving the use of electron paramagnetic resonance techniques and spin trapping to investigate the role of oxygen free radicals as well as free radicals of other compounds has increased significantly in the last few ..
  25. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2006
    ..Understanding the relationship between DOX-induced apoptosis and ROS formation may help discover novel antioxidant/antiapoptotic therapy in oxidant-induced disease processes. ..
  26. CONFERENCE/WORKSHOP ON EPR SPECTROSCOPY AND IMAGING
    Periannan Kuppusamy; Fiscal Year: 2005
    ..S. members of the International Advisory Committee for this meeting. Particular emphasis will be given to the minority and women scientists, encouraging their active participation. ..
  27. IN VIVO DETECTION OF RADIATION INDUCED FREE RADICALS
    Howard Halpern; Fiscal Year: 1999
    ..very low frequency electron paramagnetic resonance (EPR) spectroscopy in combination with new, robust in vivo spin trapping techniques...
  28. Cardiomyopathy: Pro-Oxidant Role of Zidovudine (AZT)
    William Weglicki; Fiscal Year: 2007
    ..The proposed iron chelation and antibiotic intervention strategies may lead to adjunct therapies aimed at lessen AZT-related cardiovascular toxicity. [unreadable] [unreadable]..
  29. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2007
    ..Novelty: This proposal presents a new insight on peroxide-induced iron signaling in endothelial apoptosis and on the role of NO and antioxidants in mitigating these effects. ..
  30. TOPICAL IN-VIVO ELECTRON SPIN RESONANCE
    Lawrence Berliner; Fiscal Year: 1993
    ..monitoring tumour growth characteristics by spin label metabolism c. spin trapping studies of a swine global myocardial ischemia reperfusion model; d. nitroxide monitoring in the brain.
  31. MECHANISMS OF INJURY OF MYOCARDIAL MEMBRANES AND CELLS
    William Weglicki; Fiscal Year: 1999
    ..applicant will utilize a variety of techniques to monitor: free radical production and oxidative injury (ESR spin trapping, lipid peroxidation assays, and endogenous antioxidant quantification); iron mobilization (histochemically and ..
  32. PHAGOCYTES, FREE RADICALS AND CELLULAR CONSEQUENCES
    Myron Cohen; Fiscal Year: 1991
    ..b>Spin trapping is a tool which has allowed us to measure intraphagosomal formation of free radicals. Our results show that OH...
  33. Mechanism of hypoxia mediated radiation lung injury
    Zeljko Vujaskovic; Fiscal Year: 2006
    ..ROS will be detected using electron spin resonance (ESR) spectroscopy and spin trapping. A radionuclide perfusion assay will be used to assess pulmonary perfusion...
  34. IRON DEPOSITION AND MOBILIZATION IN FERRITIN
    NORMAN CHASTEEN; Fiscal Year: 2005
    ..titration calorimetry, UV-visible stopped-flow kinetics, rapid-freeze quench Mossbauer and EPR spectroscopies, spin trapping, light scattering, oximetry and pH stat...
  35. MICROWAVE TOXICOLOGY AND MEMBRANE BARRIER CHANGES
    David Lange; Fiscal Year: 1992
    ..with changes in BBB permeability will be investigated using electron spin resonance (ESR) techniques (spin trapping and spin stabilization), lipid peroxidation assays, and free-radical scavenging techniques (superoxide ..
  36. AZULENYL NITRONES--NEW SPIN TRAPS FOR BIOLOGICAL STUDIES
    David Becker; Fiscal Year: 2001
    ..Preliminary results obtained with AZN support the principal thesis of this proposal, namely that azulenyl nitrones constitute an important new class of spin trapping agents with promising diagnostic and therapeutic potential.
  37. Novel Spin Traps for Biological Free Radical Detection
    Frederick A Villamena; Fiscal Year: 2010
    ..The synergistic application of these technologies will provide an optimal strategy to provide new materials which can be effective in probing the role of reactive oxygen species in biological mechanisms. ..
  38. Tetrahydrobiopterin in Hypoxia-Ischemia Induced Fetal Brain Dysfunction
    Jeannette Vasquez Vivar; Fiscal Year: 2008
    ..This information will likely define the use of tetrahydrobiopterin in the prevention and/or amelioration of H-I induced fetal brain dysfunction, which remains a major medical challenge. [unreadable] [unreadable] [unreadable]..
  39. Superoxide Generation from eNOS: The Role of Pterins
    JEANNETTE M VASQUEZ VIVAR; Fiscal Year: 2010
    ..Generally this proposal is aimed at understanding these fundamental mechanisms that should provide the basis for developing new and improved strategies in the prevention and treatment of atherosclerosis and CVD. ..
  40. Research Analysis Support
    David Brown; Fiscal Year: 2009
    Bioinformatics, MNR, Molecular Modeling, Protein Expression, Electron Spin Trapping
  41. MOLECULAR TARGETS OF NITRIC OXIDE IN CARDIAC TRANSPLANTS
    GALEN PIEPER; Fiscal Year: 2004
    ..resonance spectroscopy and immunoprecipitation); assay for apoptosis; quantitation of reactive oxygen (EPR spin trapping; salicylate trapping); evaluation with iNOS knockout and SOD1 or SOD2 transgenic mice...
  42. iNOS Posttranslational Regulation in Cardiac Rejection
    GALEN PIEPER; Fiscal Year: 2009
    ..A better understanding of this molecular process may lead to better strategies to prevent injury cardiac cells in these patients. ..
  43. EPR Imaging of Brain O2 in Drug Abuse
    Gerald Rosen; Fiscal Year: 2008
    ..This grant is designed to develop EPR imaging as a tool to study the effects of drugs of abuse on brain oxygen use, which may be critical to understanding brain dysfunction in drug abusing humans. [unreadable] [unreadable] [unreadable]..
  44. OMRF MRI SYSTEM
    ROBERT FLOYD; Fiscal Year: 2004
    ..Additionally, new MRI technology will be developed for assessing tissue localization of specific flee radicals with a focus on nitric oxide intermediates, and tissue-specific contrast enhancement. ..
  45. FREE RADICALS AND CHOLINE DEFICIENT LIVER CARCINOGENESIS
    ROBERT FLOYD; Fiscal Year: 2005
    ..C) We will determine if PBN (or its metabolite 4-OH-PBN) inhibits CD-mediated HCC development and ascertain if these compounds increase apoptosis in preneoplastic cells in this model. ..
  46. Polymer-linked Nitroxides Tumor-MRI Contrast Agents.
    Gerald Rosen; Fiscal Year: 2005
    ..Results from these studies will pave the way for the development of novel HPMA copolymer-linked nitroxides for MR imaging of solid tumors and concurrent monitoring of targeted cancer chemotherapy. ..
  47. Molecular Surrogate for Melanoma Prevention
    Graham Timmins; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  48. HPLC DETECTION OF RADICAL INTERMEDIATES
    Detcho Stoyanovsky; Fiscal Year: 2002
    The goal of the proposed work is to combine the ESR spin trapping and the electrochemical-HPLC techniques (EC-HPLC) for analysis of radical intermediates produced by cell organelles, intact cells and in vivo...
  49. PHOTODYNAMICS OF ULTRAVIOLET EPIDERMAL CHROMOPHORES
    John Simon; Fiscal Year: 2002
    ....
  50. BIOPHYSICAL DYNAMICS
    Robert Bryant; Fiscal Year: 2004
    ..This problem is important to understanding the spin relaxation in tissue systems, which is a valuable interpretative aid in clinical radiology. ..
  51. APOLIPOPROTEIN E AND THE ISCHEMIC BRAIN
    David Warner; Fiscal Year: 2001
    ..We believe that this information would be of substantial value in defining both the relevance of Apo E mechanisms to ischemic outcome and provide a basis for therapeutic approaches to the treatment of stroke. ..
  52. Fetal DNA oxidation and repair in neurodegeneration
    Peter Wells; Fiscal Year: 2007
    ..These studies will provide mechanistic insight into the fetal origin of environmentally-induced neurodevelopmental defects, and the role of DNA repair activity as a risk factor. ..
  53. Determination of Clinical Feasibility of EPR Oximetry of Tumors
    Harold Swartz; Fiscal Year: 2007
    ..Determining the feasibility of continuous measurements for up to 120 minutes. [unreadable] [unreadable] [unreadable]..
  54. Endogenous Radicals, Hepatic Lipid Peroxidation, and ALD
    Steven Qian; Fiscal Year: 2008
    ..Data obtained in this study are expected to lead to the submission of a future NIH RO1 grant in which radical-mediated molecular/signal pathways that may contribute to ALD will be tested. [unreadable] [unreadable] [unreadable]..
  55. PATHOGENESIS OF HERPETIC KERATITIS
    WILLIAM O BRIEN; Fiscal Year: 2002
    ..The long-term goal is to understand the pathogenesis of edema in order to develop effective and specific alternatives to corticosteroids for the therapy of HSV-induced edema and stromal disease. ..