electron spin resonance spectroscopy

Summary

Summary: A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.

Top Publications

  1. ncbi Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance
    Gunnar Jeschke
    University of Konstanz, Universitätsstrasse, 78457 Konstanz, Germany
    Phys Chem Chem Phys 9:1895-910. 2007
  2. ncbi Rotamer libraries of spin labelled cysteines for protein studies
    Yevhen Polyhach
    Laboratory of Physical Chemistry, ETH Zurich, Wolfgang Pauli Str 10, 8093 Zurich, Switzerland
    Phys Chem Chem Phys 13:2356-66. 2011
  3. ncbi The determination of pair distance distributions by pulsed ESR using Tikhonov regularization
    Yun Wei Chiang
    Baker Laboratory of Chemistry and Chemical Biology, National Biomedical ACERT Center for Advanced ESR Technology, Cornell University, Ithaca, NY 14853 1301, USA
    J Magn Reson 172:279-95. 2005
  4. ncbi Long-range distance determinations in biomacromolecules by EPR spectroscopy
    Olav Schiemann
    Institute of Physical and Theoretical Chemistry, Center for Biomolecular Magnetic Resonance, J W Goethe University Frankfurt, 60438 Frankfurt am Main, Germany
    Q Rev Biophys 40:1-53. 2007
  5. ncbi Protein structure determination using long-distance constraints from double-quantum coherence ESR: study of T4 lysozyme
    Petr P Borbat
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    J Am Chem Soc 124:5304-14. 2002
  6. pmc A scissors mechanism for stimulation of SNARE-mediated lipid mixing by cholesterol
    Jiansong Tong
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 106:5141-6. 2009
  7. ncbi Methods and applications of site-directed spin labeling EPR spectroscopy
    Candice S Klug
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Methods Cell Biol 84:617-58. 2008
  8. ncbi Counting the monomers in nanometer-sized oligomers by pulsed electron-electron double resonance
    Bela E Bode
    Institute of Physical and Theoretical Chemistry, Center for Biomolecular Magnetic Resonance, J W Goethe University, Frankfurt am Main, Germany
    J Am Chem Soc 129:6736-45. 2007
  9. ncbi Characterization of dye-decolorizing peroxidases from Rhodococcus jostii RHA1
    Joseph N Roberts
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Biochemistry 50:5108-19. 2011
  10. pmc Crystal structure and characterization of particulate methane monooxygenase from Methylocystis species strain M
    Stephen M Smith
    Departments of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, Illinois 60208, United States
    Biochemistry 50:10231-40. 2011

Detail Information

Publications408 found, 100 shown here

  1. ncbi Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance
    Gunnar Jeschke
    University of Konstanz, Universitätsstrasse, 78457 Konstanz, Germany
    Phys Chem Chem Phys 9:1895-910. 2007
    ..Interpretation of spin-to-spin distance distributions in terms of the structure of the biomacromolecules furthermore requires a model for the conformational distribution of the spin labels...
  2. ncbi Rotamer libraries of spin labelled cysteines for protein studies
    Yevhen Polyhach
    Laboratory of Physical Chemistry, ETH Zurich, Wolfgang Pauli Str 10, 8093 Zurich, Switzerland
    Phys Chem Chem Phys 13:2356-66. 2011
    ..Suggestions for the selection of labelling sites in proteins are given, limitations of the approach discussed, and requirements for further development are outlined...
  3. ncbi The determination of pair distance distributions by pulsed ESR using Tikhonov regularization
    Yun Wei Chiang
    Baker Laboratory of Chemistry and Chemical Biology, National Biomedical ACERT Center for Advanced ESR Technology, Cornell University, Ithaca, NY 14853 1301, USA
    J Magn Reson 172:279-95. 2005
    ..This study supplies a mathematically reliable method for extracting pair distributions from pulsed ESR experimental data and has extended the use of pulsed ESR to provide results of greater value for structural biology...
  4. ncbi Long-range distance determinations in biomacromolecules by EPR spectroscopy
    Olav Schiemann
    Institute of Physical and Theoretical Chemistry, Center for Biomolecular Magnetic Resonance, J W Goethe University Frankfurt, 60438 Frankfurt am Main, Germany
    Q Rev Biophys 40:1-53. 2007
    ..The main focus of this article will be on pulsed EPR methods like pulsed electron-electron double resonance (PELDOR) and their applications to spin-labeled biosystems...
  5. ncbi Protein structure determination using long-distance constraints from double-quantum coherence ESR: study of T4 lysozyme
    Petr P Borbat
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853 1301, USA
    J Am Chem Soc 124:5304-14. 2002
    ..This study sets the stage for the use of DQC-ESR for determining the tertiary structure of large proteins with just a small number of long-distance constraints...
  6. pmc A scissors mechanism for stimulation of SNARE-mediated lipid mixing by cholesterol
    Jiansong Tong
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 106:5141-6. 2009
    ..The open scissors-like conformation may be unfavorable for fusion and cholesterol may relieve this inhibitory factor...
  7. ncbi Methods and applications of site-directed spin labeling EPR spectroscopy
    Candice S Klug
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Methods Cell Biol 84:617-58. 2008
    ....
  8. ncbi Counting the monomers in nanometer-sized oligomers by pulsed electron-electron double resonance
    Bela E Bode
    Institute of Physical and Theoretical Chemistry, Center for Biomolecular Magnetic Resonance, J W Goethe University, Frankfurt am Main, Germany
    J Am Chem Soc 129:6736-45. 2007
    ..Thus, this method is a valuable tool to determine the number of constituting spin-bearing monomers in biologically relevant homo- and heterooligomers and how their oligomerization state and geometric arrangement changes during function...
  9. ncbi Characterization of dye-decolorizing peroxidases from Rhodococcus jostii RHA1
    Joseph N Roberts
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Biochemistry 50:5108-19. 2011
    ..Insight into the structure and function of DypB facilitates its engineering for the improved degradation of lignocellulose...
  10. pmc Crystal structure and characterization of particulate methane monooxygenase from Methylocystis species strain M
    Stephen M Smith
    Departments of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, Illinois 60208, United States
    Biochemistry 50:10231-40. 2011
    ..capsulatus (Bath) pMMO. In these new structures, the intramembrane zinc/copper binding site has a different coordination environment from that in previous models...
  11. pmc Conformational cycle of the ABC transporter MsbA in liposomes: detailed analysis using double electron-electron resonance spectroscopy
    Ping Zou
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 2215 Garland Avenue, Nashville, TN 37232, USA
    J Mol Biol 393:586-97. 2009
    ..Together with extensive accessibility analysis, our results suggest that these structures capture features of the motion that couples ATP energy expenditure to work, providing a framework for the mechanism of substrate transport...
  12. pmc EPR detection of cellular and mitochondrial superoxide using cyclic hydroxylamines
    Sergey I Dikalov
    FRIMCORE, Emory University School of Medicine, Atlanta, GA, USA
    Free Radic Res 45:417-30. 2011
    ..These data show that a new set of hydroxylamine spin probes provide unique information about site-specific production of the O₂ⁱ⁻ radical in extracellular or intracellular compartments, cytoplasm or mitochondria...
  13. pmc The "beta-clasp" model of apolipoprotein A-I--a lipid-free solution structure determined by electron paramagnetic resonance spectroscopy
    Jens O Lagerstedt
    Department of Experimental Medical Science, Lund University, S 221 84 Lund, Sweden
    Biochim Biophys Acta 1821:448-55. 2012
    ..This article is part of a Special Issue entitled Advances in High Density Lipoprotein Formation and Metabolism: A Tribute to John F. Oram (1945-2010)...
  14. pmc Structure of apolipoprotein A-I N terminus on nascent high density lipoproteins
    Jens O Lagerstedt
    Department of Experimental Medical Science, Lund University, S 221 84 Lund, Sweden
    J Biol Chem 286:2966-75. 2011
    ..6 nm rHDL (residues 6-98) and identified residues in this region that are affected by particle size. We conclude that the inter-helical segment (residues 35-49) plays a role in the adaptation of apoA-I to the particle size of HDL...
  15. ncbi EPR dosimetry with tooth enamel: A review
    Paola Fattibene
    Istituto Superiore di Sanita, Department of Technology and Health, Viale Regina Elena, Rome, Italy
    Appl Radiat Isot 68:2033-116. 2010
    ..Results of and problems encountered in international intercomparisons and epidemiological studies are also dealt with. In the final section the future of EPR dosimetry with tooth enamel is analyzed...
  16. ncbi Repair of oxidized iron-sulfur clusters in Escherichia coli
    Ouliana Djaman
    Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 279:44590-9. 2004
    ....
  17. ncbi Measuring distances by pulsed dipolar ESR spectroscopy: spin-labeled histidine kinases
    Peter P Borbat
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, USA
    Methods Enzymol 423:52-116. 2007
    ....
  18. ncbi Production of melanin by Aspergillus fumigatus
    Sirida Youngchim
    Dermatology Department, St Johns Institute of Dermatology, Guy s Hospital, Kings and St Thomas Medical Schools, London, UK
    J Med Microbiol 53:175-81. 2004
    ..b>Electron spin resonance spectroscopy revealed that the conidial-derived particles were stable free radicals consistent with an ..
  19. pmc Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS
    Suchandra Bhattacharjee
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 50:1536-45. 2011
    ..The DMPO adduct formed is initially EPR active but subsequently is oxidized to the stable nitrone, which can then be detected by IST and further characterized by MS and MS/MS...
  20. ncbi Protein modulation of lipids, and vice-versa, in membranes
    Derek Marsh
    Max Planck Institut fur biophysikalische Chemie, Abt Spektroskopie, Gottingen, Germany
    Biochim Biophys Acta 1778:1545-75. 2008
    ....
  21. ncbi Glucocorticoids modulate tumor radiation response through a decrease in tumor oxygen consumption
    Nathalie Crokart
    Laboratories of Medicinal Chemistry and Radiopharmacy, Universite Catholique de Louvain, B 1200 Brussels, Belgium
    Clin Cancer Res 13:630-5. 2007
    ..We hypothesized that glucocorticoids may enhance tumor radiosensitivity by increasing tumor oxygenation (pO(2)) through inhibition of mitochondrial respiration...
  22. pmc Honokiol is a potent scavenger of superoxide and peroxyl radicals
    Sergey Dikalov
    Division of Cardiology, Emory University School of Medicine, 1639 Pierce Drive, Suite 319 WMB, Atlanta, GA 30322, United States
    Biochem Pharmacol 76:589-96. 2008
    ..5x10(6)M(-1)s(-1)). Therefore, honokiol is an effective scavenger of both superoxide and peroxyl radicals, which may be important for physiological activity of honokiol...
  23. pmc Coordination features and affinity of the Cu²+ site in the α-synuclein protein of Parkinson's disease
    Christopher G Dudzik
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, United States
    Biochemistry 50:1771-7. 2011
    ....
  24. ncbi A tyrosyl-dimanganese coupled spin system is the native metalloradical cofactor of the R2F subunit of the ribonucleotide reductase of Corynebacterium ammoniagenes
    Nicholas Cox
    Max Planck Institut fur Bioanorganische Chemie, Stiftstrasse 34 36, D 45470 Mulheim Ruhr, Germany
    J Am Chem Soc 132:11197-213. 2010
    ..The presented results demonstrate the importance of manganese for radical formation in this RNR and confirm the assignment of this enzyme to class Ib...
  25. ncbi A novel electron paramagnetic resonance approach to determine the mechanism of drug transport by P-glycoprotein
    Hiroshi Omote
    Department of Molecular Physiology and Biological Physics, University of Virginia Health System, Charlottesville, Virginia 22908 0736, USA
    J Biol Chem 277:45688-94. 2002
    ..Steady state gradients of spin-labeled verapamil within the range of K(i)/K(m) ratios were observed...
  26. ncbi The structure of apolipoprotein A-I in high density lipoproteins
    W Sean Davidson
    Department of Pathology and Laboratory Medicine, University of Cincinnati, OH 45237, USA
    J Biol Chem 282:22249-53. 2007
  27. ncbi Reconstruction of the chemotaxis receptor-kinase assembly
    Sang Youn Park
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA
    Nat Struct Mol Biol 13:400-7. 2006
    ..Linking layers of ternary complexes with close-packed receptors generates a lattice with reasonable component ratios, cooperative interactions among receptors and accessible sites for modification enzymes...
  28. pmc Sugar binding induces an outward facing conformation of LacY
    Irina Smirnova
    Department of Physiology, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 104:16504-9. 2007
    ..The DEER measurements in conjunction with molecular modeling based on the x-ray structure provide strong support for the alternative access model and reveal a structure for the outward-facing conformer of LacY...
  29. ncbi Estimation of inter-residue distances in spin labeled proteins at physiological temperatures: experimental strategies and practical limitations
    C Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-7008, USA
    Biochemistry 40:15471-82. 2001
    ..The existence of such distance distributions places important constraints on the interpretation of internitroxide distances in terms of protein structure and structural changes...
  30. ncbi Chlorite dismutase from Ideonella dechloratans
    K Stenklo
    Department of Chemistry, Karlstad University, Sweden
    J Biol Inorg Chem 6:601-7. 2001
    ..In the alkaline pH range, a low-spin compound is formed. A 22-residue N-terminal amino acid sequence has been determined and no homologue has been found in the protein sequence databases...
  31. ncbi Biochemical and structural insights into bacterial organelle form and biogenesis
    Joshua B Parsons
    Protein Science Group, Department of Biochemistry, University of Kent, Canterbury, Kent, UK
    J Biol Chem 283:14366-75. 2008
    ..The recombinant model will facilitate future experiments establishing the structure and assembly of these multiprotein assemblages and their fate when the specific metabolic function is no longer required...
  32. pmc Assessing oligomerization of membrane proteins by four-pulse DEER: pH-dependent dimerization of NhaA Na+/H+ antiporter of E. coli
    Daniel Hilger
    Ludwig Maximilians Universitat Munchen, Department Biologie I, Germany
    Biophys J 89:1328-38. 2005
    ....
  33. ncbi Interplay of IscA and IscU in biogenesis of iron-sulfur clusters
    Juanjuan Yang
    Department of Biological Sciences, Louisiana State University, Baton Rouge, 70803, USA
    J Biol Chem 281:27956-63. 2006
    ..The results suggest an intriguing interplay between IscA and IscU in which IscA acts as an iron chaperon that recruits "free" iron and delivers the iron for biogenesis of iron-sulfur clusters in IscU under aerobic conditions...
  34. ncbi Maximum entropy: a complement to Tikhonov regularization for determination of pair distance distributions by pulsed ESR
    Yun Wei Chiang
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    J Magn Reson 177:184-96. 2005
    ..Thus we regard the MEM method as a complement to TIKR by securing a positive pair distance distribution and enhancing the accuracy of TIKR...
  35. ncbi Spin labeling EPR
    Johann P Klare
    Physics Department, University of Osnabruck, Barbarastr 7, 49076, Osnabruck, Germany
    Photosynth Res 102:377-90. 2009
    ....
  36. ncbi Immunochemical detection of hemoglobin-derived radicals formed by reaction with hydrogen peroxide: involvement of a protein-tyrosyl radical
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27713, USA
    Free Radic Biol Med 34:830-9. 2003
    ....
  37. ncbi Spin label EPR-based characterization of biosystem complexity
    Janez Strancar
    Laboratory of Biophysics, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    J Chem Inf Model 45:394-406. 2005
    ....
  38. ncbi Yarrowia lipolytica, a yeast genetic system to study mitochondrial complex I
    Stefan Kerscher
    Universitatsklinikum Frankfurt, Institut fur Biochemie I, Zentrum der Biologischen Chemie, Theodor Stern Kai 7, Haus 25 B, D 60590 Frankfurt am Main, Germany
    Biochim Biophys Acta 1555:83-91. 2002
    ..lipolytica complex I. Characterization of a number of mutations already allowed for the identification of several functionally important amino acids, demonstrating the usefulness of this approach...
  39. ncbi Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1
    Peter L Hagedoorn
    Kluyver Department of Biotechnology, Delft University of Technology, The Netherlands
    Eur J Biochem 269:4905-11. 2002
    ..The spectroscopic features and redox properties of chlorite dismutase are more similar to the gas-sensing hemoproteins, such as guanylate cyclase and the globins, than to the heme enzymes...
  40. ncbi The first direct characterization of a high-valent iron intermediate in the reaction of an alpha-ketoglutarate-dependent dioxygenase: a high-spin FeIV complex in taurine/alpha-ketoglutarate dioxygenase (TauD) from Escherichia coli
    John C Price
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 42:7497-508. 2003
    ..The detection of this intermediate sets the stage for a more detailed dissection of the TauD reaction mechanism than has previously been reported for any other member of this important enzyme family...
  41. ncbi Position of synaptotagmin I at the membrane interface: cooperative interactions of tandem C2 domains
    Dawn Z Herrick
    Department of Chemistry and Biophysics Program, University of Virginia, Charlottesville, USA
    Biochemistry 45:9668-74. 2006
    ..These data indicate that C2A and C2B do not act independently but influence their mutual membrane penetration. This may explain the occurrence of multiple C2 domains in proteins that function in membrane trafficking and repair...
  42. ncbi Asymmetry in the structure of the ABC transporter-binding protein complex BtuCD-BtuF
    Rikki N Hvorup
    Institute of Molecular Biology and Biophysics, ETH Zurich, HPK D14 3, 8093 Zurich, Switzerland
    Science 317:1387-90. 2007
    ..A comparison with BtuCD and the homologous HI1470/71 protein suggests that the structure of BtuCD-F may reflect a posttranslocation intermediate...
  43. pmc Distinct roles of Nox1 and Nox4 in basal and angiotensin II-stimulated superoxide and hydrogen peroxide production
    Sergey I Dikalov
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, 1639 Pierce Drive, Atlanta, GA 30322, USA
    Free Radic Biol Med 45:1340-51. 2008
    ..These findings also provide important insight into the origin of H2O2 in vascular cells, and may partially account for the limited pharmacological effect of antioxidant treatments with O2*- scavengers that do not affect H2O2...
  44. ncbi Analysis of conformational changes in the DNA junction-resolving enzyme T7 endonuclease I on binding a four-way junction using EPR
    Alasdair D J Freeman
    Nucleic Acid Structure Research Group, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Biochemistry 50:9963-72. 2011
    ..These studies reveal that structural changes in both DNA and protein occur in the action of this junction-resolving enzyme...
  45. pmc Nanometer distance measurements in RNA using site-directed spin labeling
    Qi Cai
    Department of Chemistry, University of Southern California, Los Angeles, CA 90089 0744, USA
    Biophys J 93:2110-7. 2007
    ..The results enable future studies of global RNA structures for which high-resolution structural data are absent...
  46. ncbi Tea catechins protect against lead-induced cytotoxicity, lipid peroxidation, and membrane fluidity in HepG2 cells
    Liuji Chen
    Department of Tea Science, Zhejiang University, Hangzhou, China 310029
    Toxicol Sci 69:149-56. 2002
    ..The results suggest that tea catechin supplementation may have a role to play in modulating oxidative stress in lead-exposed HepG2 cells...
  47. pmc Electron paramagnetic resonance dosimetry for a large-scale radiation incident
    Harold M Swartz
    Dartmouth Medical School, Hanover, NH 03755, USA
    Health Phys 103:255-67. 2012
    ..The authors conclude that electron paramagnetic resonance dosimetry is likely to be a useful part of triage for a large-scale radiation incident...
  48. pmc Characterization of NocL involved in thiopeptide nocathiacin I biosynthesis: a [4Fe-4S] cluster and the catalysis of a radical S-adenosylmethionine enzyme
    Qi Zhang
    State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
    J Biol Chem 286:21287-94. 2011
    ....
  49. ncbi Electron paramagnetic resonance spectroscopy of site-directed spin labels reveals the structural heterogeneity in the N-terminal domain of apoA-I in solution
    Jens O Lagerstedt
    Department of Biochemistry, University of California, Davis, California 95616, USA
    J Biol Chem 282:9143-9. 2007
    ..Based on intermolecular dipolar coupling at positions 26, 44, and 64, these secondary structural elements were arranged into a tertiary fold to generate a structural model for lipid-free apoA-I in solution...
  50. pmc Antiangiogenic agent sunitinib transiently increases tumor oxygenation and suppresses cycling hypoxia
    Shingo Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 71:6350-9. 2011
    ..Overall, the findings suggest that longitudinal and noninvasive monitoring of tumor pO(2) makes it possible to identify a window of vascular renormalization to maximize the effects of combination therapy with antiangiogenic drugs...
  51. ncbi Three-spin correlations in double electron-electron resonance
    Gunnar Jeschke
    ETH Zurich, CH 8093 Zurich, Switzerland
    Phys Chem Chem Phys 11:6580-91. 2009
    ..The agreement with the expected side lengths of the triangles is satisfying...
  52. pmc Photolysis of hydrogen peroxide, an effective disinfection system via hydroxyl radical formation
    Hiroyo Ikai
    New Industry Creation Hatchery Center, Tohoku University, Aoba 6 6 10, Aramaki, Aoba ku, Sendai 980 8579, Japan
    Antimicrob Agents Chemother 54:5086-91. 2010
    ..Other results demonstrated that the bactericidal activity was dependent on the amount of hydroxyl radicals generated. Treatment of bacteria with 200 to 300 μM hydroxyl radicals would result in reductions of viable counts of >99.99%...
  53. ncbi DFT investigation of the molybdenum cofactor in periplasmic nitrate reductases: structure of the Mo(V) EPR-active species
    Frédéric Biaso
    Unite de Bioenergetique et Ingenierie des Proteines, UMR 7281, Centre National de la Recherche Scientifique, Institut de Microbiologie de la Mediterranee, and Aix Marseille University, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Inorg Chem 51:3409-19. 2012
    ..Beyond the periplasmic nitrate reductase case, this work provides useful magneto-structural correlations to characterize EPR-detected species in mononuclear molybdoenzymes...
  54. ncbi Synthesis of spin-labeled riboswitch RNAs using convertible nucleosides and DNA-catalyzed RNA ligation
    Lea Büttner
    Research Group Nucleic Acid Chemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Göttingen 37077, Germany
    Bioorg Med Chem 21:6171-80. 2013
    ....
  55. ncbi Electron paramagnetic resonance as a unique tool for skin and hair research
    Przemyslaw M Plonka
    Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Exp Dermatol 18:472-84. 2009
    ..A part of the paper is devoted to hair and nail research. The review of dermatological applications of EPR is supplemented with a handful of advice concerning practical aspects of EPR experimentation and usage of EPR reagents...
  56. pmc Backbone structure of transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli
    Daniel Hilger
    Department of Biology I, Microbiology, Ludwig Maximilians Universitat Munich, Munich, Germany
    Biophys J 96:217-25. 2009
    ..These models exhibit a pronounced kink near residue T341, which is involved in substrate binding. The kink may be associated with a hinge that allows the protein to open and close an inwardly oriented cavity...
  57. pmc Both maltose-binding protein and ATP are required for nucleotide-binding domain closure in the intact maltose ABC transporter
    Cedric Orelle
    Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 105:12837-42. 2008
    ..We propose that P(i) release coincides with the reorientation of transmembrane helices to an inward-facing conformation and the final step of maltose translocation into the cell...
  58. pmc Rigid spin-labeled nucleoside C: a nonperturbing EPR probe of nucleic acid conformation
    Pavol Cekan
    University of Iceland, Science Institute, Dunhaga 3, 107 Reykjavik, Iceland
    Nucleic Acids Res 36:5946-54. 2008
    ..These data are consistent with end-to-end duplex stacking in solution, which was only observed when G was paired to C, but not when C was paired with A, C or T...
  59. ncbi Control of blood pressure variability in caveolin-1-deficient mice: role of nitric oxide identified in vivo through spectral analysis
    Fanny Desjardins
    Unit of Pharmacology and Therapeutics, Universite Catholique de Louvain, UCL FATH 5349, Vésale 5, 52 Avenue Mounier, 1200 Brussels, Belgium
    Cardiovasc Res 79:527-36. 2008
    ..However, the impact of genetic alteration of cav-1 on vascular nitric oxide (NO) production and BP homeostasis in vivo is unknown...
  60. pmc Lesion-induced DNA weak structural changes detected by pulsed EPR spectroscopy combined with site-directed spin labelling
    Giuseppe Sicoli
    Laboratoire de Résonance Magnétique, UMR E no 3 CEA UJF FRE 3200 CNRS Institut des Nanosciences et Cryogénie, CEA Grenoble, 17, avenue des Martyrs, F 38054, Grenoble Cedex 9, France
    Nucleic Acids Res 37:3165-76. 2009
    ..Thus, the experimental approaches described herein provide an efficient method for probing lesion-induced structural changes of nucleic acids...
  61. pmc Simultaneous molecular imaging of redox reactions monitored by Overhauser-enhanced MRI with 14N- and 15N-labeled nitroxyl radicals
    Hideo Utsumi
    Department of Bio functional Science, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi ku, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 103:1463-8. 2006
    ..Thus, it may become a powerful tool to clarify mechanisms of disease and to monitor pharmaceutical therapy...
  62. ncbi Site-directed spin labeling of DNA reveals mismatch-induced nanometer distance changes between flanking nucleotides
    Dorith Wunnicke
    Department of Physics, University Osnabruck, Barbarastraße 7, 49076 Osnabruck, Germany
    J Phys Chem B 116:4118-23. 2012
    ..Pulse EPR experiments reveal that the mismatch-induced local conformational changes are transmitted to the flanking nucleotides and that the impact of this mismatch depends on the nearest neighbor environment...
  63. pmc Enzymatic activities of isolated cytochrome bc₁-like complexes containing fused cytochrome b subunits with asymmetrically inactivated segments of electron transfer chains
    Monika Czapla
    Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Biochemistry 51:829-35. 2012
    ....
  64. pmc Low-field magnetic resonance imaging to visualize chronic and cycling hypoxia in tumor-bearing mice
    Hironobu Yasui
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1002, USA
    Cancer Res 70:6427-36. 2010
    ..This EPRI technique, combined with MRI, may offer a powerful clinical tool to noninvasively detect variable oxygenation in tumors...
  65. pmc Electron paramagnetic resonance characterization of tetrahydrobiopterin radical formation in bacterial nitric oxide synthase compared to mammalian nitric oxide synthase
    Albane Brunel
    CNRS, Laboratoire Stress Oxydant et Détoxication, Gif sur Yvette, France
    Biophys J 103:109-17. 2012
    ..Our data highlight potential differences for the catalytic mechanism of NOHA oxidation between mammalian and bacterial NOSs...
  66. pmc TNF-induced mitochondrial damage: a link between mitochondrial complex I activity and left ventricular dysfunction
    Nithya Mariappan
    Department of Comparative Biomedical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USA
    Free Radic Biol Med 46:462-70. 2009
    ....
  67. pmc Algorithm for selection of optimized EPR distance restraints for de novo protein structure determination
    Kelli Kazmier
    Chemical and Physical Biology Program, 340 Light Hall, Vanderbilt University, Nashville, TN 37232, USA
    J Struct Biol 173:549-57. 2011
    ....
  68. pmc New radiometric ages for the BH-1 hominin from Balanica (Serbia): implications for understanding the role of the Balkans in Middle Pleistocene human evolution
    William J Rink
    School of Geography and Earth Sciences, McMaster University, Hamilton, Ontario, Canada
    PLoS ONE 8:e54608. 2013
    ....
  69. pmc RosettaEPR: an integrated tool for protein structure determination from sparse EPR data
    Stephanie J Hirst
    Center for Structural Biology, Vanderbilt University, Nashville, TN 37212, USA
    J Struct Biol 173:506-14. 2011
    ..In order to affirm the method's general performance, it must be tested on a larger and more versatile dataset of proteins...
  70. pmc UV effects in tooth enamel and their possible application in EPR dosimetry with front teeth
    S Sholom
    Laboratory of External Exposure Dosimetry, Research Center for Radiation Medicine, Kiev, Ukraine
    Health Phys 98:360-8. 2010
    ..The technique developed here to quantify and subtract the UV contribution to the measured tooth is currently limited to cumulative dose measurements with a component of UV equivalent dose equal to or greater than 300 mGy...
  71. pmc Interaction of a G protein with an activated receptor opens the interdomain interface in the alpha subunit
    Ned Van Eps
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 7008, USA
    Proc Natl Acad Sci U S A 108:9420-4. 2011
    ..The interdomain opening is coupled to receptor binding via the C-terminal helix of G(α), the extension of which is a high-affinity receptor binding element...
  72. ncbi Oxidative stress and pathogenesis of inflammatory bowel disease: an epiphenomenon or the cause?
    Ali Rezaie
    Department of Community Health Medicine, Faculty of Medicine, University of Calgary, Calgary, Canada
    Dig Dis Sci 52:2015-21. 2007
    ..Moreover, the effects of antioxidant therapy on IBD patients in randomized, controlled trials were reviewed and the need for further studies elaborated. We also summarized the evidence in support for causality of oxidative stress in IBD...
  73. ncbi Dark production of reactive oxygen species in photosystem II membrane particles at elevated temperature: EPR spin-trapping study
    Pavel Pospisil
    Laboratory of Biophysics, Department of Experimental Physics, Faculty of Science, Palacky University, tr Svobody 26, 771 46 Olomouc, Czech Republic
    Biochim Biophys Acta 1767:854-9. 2007
    ..scavengers. This finding indicates that the production of these two species was independent and that the production of (1)O2 is not closely linked to PSII donor side...
  74. pmc Studying RNA using site-directed spin-labeling and continuous-wave electron paramagnetic resonance spectroscopy
    Xiaojun Zhang
    Department of Chemistry, University of Southern California, Los Angeles, California, USA
    Methods Enzymol 469:303-28. 2009
    ..Specific examples are subsequently used to illustrate analysis of EPR spectra, showing how information regarding the parent RNA can be extracted...
  75. pmc Endothelial dihydrofolate reductase: critical for nitric oxide bioavailability and role in angiotensin II uncoupling of endothelial nitric oxide synthase
    Karel Chalupsky
    Section of Cardiology, Department of Medicine, Division of Biological Sciences and Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 102:9056-61. 2005
    ..This signaling cascade may represent a universal mechanism underlying eNOS dysfunction under pathophysiological conditions associated with oxidant stress...
  76. ncbi Probing structural transitions in both structured and disordered proteins using site-directed spin-labeling EPR spectroscopy
    Sonia Longhi
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS and Aix Marseille Université, 163 Avenue de Luminy, Case 932, 13288 Marseille Cedex 09, France
    J Pept Sci 17:315-28. 2011
    ....
  77. pmc ESR evidence for 2 coexisting liquid phases in deeply supercooled bulk water
    D Banerjee
    Department of Physics, Indian Institute of Science, Bangalore 560 012, India
    Proc Natl Acad Sci U S A 106:11448-53. 2009
    Using electron spin resonance spectroscopy (ESR), we measure the rotational mobility of probe molecules highly diluted in deeply supercooled bulk water and negligibly constrained by the possible ice fraction...
  78. ncbi Amplitude of pancreatic lipase lid opening in solution and identification of spin label conformational subensembles by combining continuous wave and pulsed EPR spectroscopy and molecular dynamics
    Sebastien Ranaldi
    CNRS Laboratoire de Bioenergetique et Ingenierie des Proteines, UPR 9036, Institut de Microbiologie de la Mediterranee, Aix Marseille Universités, Marseille, France
    Biochemistry 49:2140-9. 2010
    ....
  79. ncbi Mapping global folds of oligonucleotides by pulsed electron-electron double resonance
    Olav Schiemann
    Centre for Biomolecular Sciences, Centre of Magnetic Resonance, University of St Andrews, St Andrews, United Kingdom
    Methods Enzymol 469:329-51. 2009
    ..This chapter briefly summarizes the theory, describes how to perform and analyze such experiments and discusses the limitations...
  80. pmc Spectroscopic validation of the pentameric structure of phospholamban
    Nathaniel J Traaseth
    Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 104:14676-81. 2007
    ..We propose that the interaction with the bilayer precedes SERCA binding and may mediate the interactions with other proteins such as protein kinase A and protein phosphatase 1...
  81. ncbi Rigid body refinement of protein complexes with long-range distance restraints from pulsed dipolar ESR
    Jaya Bhatnagar
    Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, USA
    Methods Enzymol 423:117-33. 2007
    ..Pulsed dipolar electron spin resonance spectroscopy is one such powerful technique for obtaining long-range distance restraints in protein complexes...
  82. pmc HF-EPR, Raman, UV/VIS light spectroscopic, and DFT studies of the ribonucleotide reductase R2 tyrosyl radical from Epstein-Barr virus
    Ane B Tomter
    Department of Molecular Biosciences, University of Oslo, Oslo, Norway
    PLoS ONE 6:e25022. 2011
    ..Comparison with previous studies of RNR R2s from mouse, bacteria, and herpes viruses, demonstrates that finely tuned electronic properties of the radical exist within the same RNR R2 Ia class...
  83. pmc Solid-support electron paramagnetic resonance (EPR) studies of Aβ40 monomers reveal a structured state with three ordered segments
    Lei Gu
    Department of Neurology, Brain Research Institute, Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    J Biol Chem 287:9081-9. 2012
    ..The structured state of Aβ40 monomer has three more ordered segments at 14-18, 29-30, and 38-40. Interactions between these segments may stabilize the structured state, which likely plays an important role in Aβ aggregation...
  84. ncbi PELDOR study of conformations of double-spin-labeled single- and double-stranded DNA with non-nucleotide inserts
    Nikita A Kuznetsov
    Institute of Chemical Biology and Fundamental Medicine, Lavrentyev Ave 8, 630090, Novosibirsk, Russia
    Phys Chem Chem Phys 11:6826-32. 2009
    ..The results evidence that PELDOR of spin-labeled DNA molecules may be used as a "molecular ruler" for studying the influence of local damages on the DNA conformations...
  85. ncbi Kinetic and stoichiometric assessment of the antioxidant activity of flavonoids by electron spin resonance spectroscopy
    Donald B McPhail
    Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, Scotland
    J Agric Food Chem 51:1684-90. 2003
    ..However, rates and stoichiometries did not always correlate and the data suggest that kinetic factors may be of greater importance within a biological context...
  86. pmc Synergistic effect of thermal energy on bactericidal action of photolysis of H₂O₂ in relation to acceleration of hydroxyl radical generation
    Midori Shirato
    Division of Fixed Prosthodontics, Department of Restorative Dentistry, Tohoku University Graduate School of Dentistry, Seiryou 4 1, Aoba ku, Sendai 980 8575, Japan
    Antimicrob Agents Chemother 56:295-301. 2012
    ..These findings suggest that the thermal energy accelerates the generation of hydroxyl radical by photolysis of H(2)O(2), which in turn results in a synergistic bactericidal effect of hydroxyl radical and thermal energy...
  87. ncbi Evaluation of spin labels for in-cell EPR by analysis of nitroxide reduction in cell extract of Xenopus laevis oocytes
    Mykhailo Azarkh
    University of Konstanz, Departments of Chemistry and Biology, Konstanz Research School Chemical Biology KoRS CB, Zukunftskolleg, 78457 Konstanz, Germany
    J Magn Reson 212:450-4. 2011
    ..The kinetic data can be described according to the Michaelis-Menten model and thus suggest an enzymatic or enzyme-mediated reduction process...
  88. pmc Monomeric and dimeric conformation of the vinculin tail five-helix bundle in solution studied by EPR spectroscopy
    Christoph Abé
    Department of Physics, University of Osnabruck, Osnabruck, Germany
    Biophys J 101:1772-80. 2011
    ..Furthermore, the distance between positions 1033 is shown to increase by >3 nm upon interaction of the vinculin tail domain with F-actin...
  89. pmc Significantly improved sensitivity of Q-band PELDOR/DEER experiments relative to X-band is observed in measuring the intercoil distance of a leucine zipper motif peptide (GCN4-LZ)
    Harishchandra Ghimire
    Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056, USA
    Biochemistry 48:5782-4. 2009
    ..Thus, the availability of Q-band DEER spectroscopy should have a major impact on structural biology studies using site-directed spin labeling EPR techniques...
  90. ncbi Studying biomolecular complexes with pulsed electron-electron double resonance spectroscopy
    Gunnar W Reginsson
    Biomedical Sciences Research Complex, Centre of Magnetic Resonance, University of St Andrews, St Andrews KY16 9ST, UK
    Biochem Soc Trans 39:128-39. 2011
    ..In the present review, the methods of PELDOR are discussed and examples where PELDOR has been used to obtain structural information on biomolecules are summarized...
  91. pmc High-resolution distance mapping in rhodopsin reveals the pattern of helix movement due to activation
    Christian Altenbach
    Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 105:7439-44. 2008
    ..The positions of nitroxides in other helices at the cytoplasmic surface remained largely unchanged...
  92. ncbi Spectral and thermodynamic properties of methanobactin from γ-proteobacterial methane oxidizing bacteria: a case for copper competition on a molecular level
    Dong W Choi
    Department of Biochemistry, Biophysics and Molecular Biology Iowa State University, Ames, IA 50011 3211, United States
    J Inorg Biochem 104:1240-7. 2010
    ..trichosporium OB3b removed Cu(I) from the mb of both γ-proteobacterial MOB. Taken together the results suggest mb may be a factor in regulating methanotrophic community structure in copper-limited environments...
  93. pmc Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice
    Saurabh Chatterjee
    Free Radical Metabolites Group, Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Free Radic Biol Med 46:454-61. 2009
    ..This suggests the presence of a possible mechanism for CPB1 activity loss with compensatory protein production...
  94. ncbi Biogenesis of a respiratory complex is orchestrated by a single accessory protein
    Pascal Lanciano
    Laboratoire de Chimie Bacterienne, Institut de Biologie Structurale et Microbiologie, CNRS, Université de Provence Aix Marseille I, Marseille Cedex 09, France
    J Biol Chem 282:17468-74. 2007
    ..Our findings demonstrate that NarJ has a multifunctional role by orchestrating both the maturation and the assembly steps...
  95. ncbi Pulsed ELDOR determination of the intramolecular distance between the metal binding sites in Dicupric human serum transferrin and lactoferrin
    Christopher W M Kay
    Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
    J Am Chem Soc 129:4868-9. 2007
  96. pmc Molecular details of Bax activation, oligomerization, and membrane insertion
    Stephanie Bleicken
    Department of Membrane Biochemistry, Max Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Biol Chem 285:6636-47. 2010
    ....
  97. pmc Phosphatidylinositol 4,5-bisphosphate alters synaptotagmin 1 membrane docking and drives opposing bilayers closer together
    Weiwei Kuo
    Department of Chemistry and Center for Membrane Biology, University of Virginia, Charlottesville, Virginia 22904 4319, United States
    Biochemistry 50:2633-41. 2011
    ..The results indicate that PIP(2), which is necessary for bilayer fusion, alters C2 domain orientation, enhances syt1-membrane electrostatic interactions, and acts to drive vesicle and cytoplasmic membrane surfaces closer together...
  98. pmc Membrane docking geometry and target lipid stoichiometry of membrane-bound PKCα C2 domain: a combined molecular dynamics and experimental study
    Chun Liang Lai
    Department of Chemistry, James Franck Institute, and Computation Institute, University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA
    J Mol Biol 402:301-10. 2010
    ..Thus, the experiment confirms the target lipid stoichiometry predicted by EPR-guided molecular dynamics simulations. Potential biological implications of the observed docking geometries and PIP(2) stoichiometries are discussed...
  99. pmc Modulation of the ligand-field anisotropy in a series of ferric low-spin cytochrome c mutants derived from Pseudomonas aeruginosa cytochrome c-551 and Nitrosomonas europaea cytochrome c-552: a nuclear magnetic resonance and electron paramagnetic resonance
    Giorgio Zoppellaro
    Department of Molecular Biosciences, University of Oslo, Post Office Box 1041 Blindern, Oslo NO 0316, Norway
    J Am Chem Soc 130:15348-60. 2008
    ..Thus, for these His-Met axially coordinated Fe(III), the large g(max) value EPR signal does not represent a special case as is observed for bis-His axially coordinated Fe(III) with the two His planes perpendicular to each other...
  100. ncbi Structural characterization of beta-amyloid oligomer-aggregates by ion mobility mass spectrometry and electron spin resonance spectroscopy
    Marius Ionuţ Iuraşcu
    Laboratory of Analytical Chemistry and Biopolymer Structure Analysis, Department of Chemistry, University of Konstanz, 78457, Konstanz, Germany
    Anal Bioanal Chem 395:2509-19. 2009
    ..The EPR spectra of the spin-labeled Cys-Abeta(1-40) oligomers were matched with spectra simulations using a multi-component simulation strategy, resulting in complete agreement with the gel electrophoresis results...
  101. pmc The iron-sulphur protein Ind1 is required for effective complex I assembly
    Katrine Bych
    Department of Plant Sciences, University of Cambridge, Cambridge, UK
    EMBO J 27:1736-46. 2008
    ..Our data suggest that Ind1 facilitates the assembly of Fe-S cofactors and subunits of complex I...

Research Grants62

  1. Prevention of UV-induced carcinogenesis by cyanidin-3-glucoside
    Xianglin Shi; Fiscal Year: 2013
    ..Present study attempts to identify cyanidin-3-glucoside, a plant-derived compound, as a mechanism-based preventive agent against UV-induced skin cancers. ..
  2. A new ultra-low field in-vivo EPR technology for biomedical applications
    Inseob Hahn; Fiscal Year: 2012
    ....
  3. A new ultra-low field in-vivo EPR technology for biomedical applications
    Inseob Hahn; Fiscal Year: 2011
    ....
  4. Apple peel extract inhibits Cr(VI)-induced lung cancer
    Zhuo Zhang; Fiscal Year: 2013
    ..In this aim we will Investigate the protection of apple peel extract against Cr(VI)-induced inflammation, chronic oxidative lung injury, and cell proliferation using female BALB/c mice. ..
  5. RPE Phenotype and Oxidative Stress
    Janice M Burke; Fiscal Year: 2013
    ..Antioxidants will also be tested to see if binding them to organelles helps the RPE sustain normal organelle motility despite ongoing photic stress associated with aging. ..
  6. Quercitrin functions as an antioxidant and protects UV-induced carcinogenesis
    Zhuo Zhang; Fiscal Year: 2012
    ..This proposal will test the hypothesis that quercitrin, a compound found in blackberries and other foods, functions as an antioxidant and protects UV-induced carcinogenesis. ..
  7. BIOLOGICAL PROPERTIES OF RPE MELANIN
    Janice M Burke; Fiscal Year: 2010
    ..The effects of age on melanosomes will also be studied to determine whether their ability to protect RPE cells from stress declines over time, which could contribute to diseases of the retina such as age-related macular degeneration. ..
  8. STRUCTURE-FUNCTION RELATIONSHIPS OF IMMUNORECEPTORS
    Barbara A Baird; Fiscal Year: 2013
    ....
  9. Center for Biophysical Assessment and Risk Management Following Irradiation
    Paul Okunieff; Fiscal Year: 2009
    ..abstract_text> ..
  10. ELECTRON SPIN RELAXATION IN MODEL MEMBRANES
    Jack H Freed; Fiscal Year: 2013
    ..Possible clinical applications include detection of membrane changes during immune response, prevention of viral entry, neurological disorders, and development of anti-microbials. ..
  11. Alpha-Crystallin and Cataractogensis
    Joseph Horwitz; Fiscal Year: 2009
    ..These are excellent models for understanding the biochemical and biophysical changes that occur in age-related human cataract. ..
  12. TRAINING IN MOLECULAR PHYSICS OF BIOLOGICAL SYSTEMS
    Michelle D Wang; Fiscal Year: 2013
    ..using theoretical approaches;structure determination by synchrotron X- ray crystallography;electron spin resonance spectroscopy and multidimensional NMR;single molecule studies of dynamics of molecular motors;structures and ..
  13. Interface Quality Effects in Phospholipase Membrane Enzymology
    Radha Ranganathan; Fiscal Year: 2013
    ..This is of importance to human health because the products of hydrolysis perform several physiological functions including cell signaling, inflammation, allergy, apoptosis, and tumorigenesis. ..
  14. Research Training Program in Vision Science
    Joseph C Besharse; Fiscal Year: 2012
    ..imaging, adaptive optics, optical coherence tomography, functional magnetic resonance imaging, and electron spin resonance spectroscopy. Pre-doctoral trainees will complete a core curriculum of Molecular Genetics, Biochemistry, Cell ..
  15. Allosteric Mechanism of Hsp70 Molecular Chaperones
    Lila M Gierasch; Fiscal Year: 2013
    ..These insights are critical for optimal use of Hsp70s as drug targets. ..
  16. ELECTRON PARAMAGNETIC RESONANCE SPECTROMETER
    Cecilia Giulivi; Fiscal Year: 2009
    ....
  17. Refinement of LC/ESR/MS for Studying Lipid Free Radicals in Colon Cancer Growth
    Steven Qian; Fiscal Year: 2009
    ..We anticipate that the outcome of our research will open a new door into knowledge of PUFA peroxidation in cancer biology and will revolutionize how we use our knowledge of enzymatic peroxidation to control colon cancer. ..
  18. Synthesis and Evaluation of Nano-antioxidants for Medical Applications
    Choon Lee; Fiscal Year: 2009
    ....
  19. ALTERED MEMBRANE PHOSPHOLIPIDS AND CARDIAC FUNCTION
    DIANE REIBEL; Fiscal Year: 1990
    ..in these isolated subcellular fractions and membrane structure (fluidity) will be evaluated using electron spin resonance spectroscopy. A variety of membrane and membrane-related functions will be studied in isolated subcellular ..
  20. CELLULAR UPTAKE AND INTERACTION OF IRON AND GALLIUM
    Christopher Chitambar; Fiscal Year: 1992
    ..Transferrin, transferrin receptor and ferritin will be immunoprecipitated by specific antibodies. Electron spin resonance spectroscopy and high performance liquid chromatography will be used to study the effect of gallium on ..
  21. Cantilever Magnetic Resonance of Biomolecules
    John A Marohn; Fiscal Year: 2012
    ..abstract_text> ..
  22. IN VIVO DETECTION OF FREE RADICALS USING NMR
    Thomas Clanton; Fiscal Year: 2000
    ..abstract_text> ..
  23. EICOSAPENTAENOIC ACID: MECHANISM OF ACTION IN PLATELETS
    MANFRED STEINER; Fiscal Year: 1990
    ..These studies provide information on the role which the bio-physical structure of EPA plays in mediating platelet function and enhance our understanding of the platelet response to agonists...
  24. MECHANISMS OF MICROWAVE DAMAGE IN OCULAR TISSUE
    David Lange; Fiscal Year: 1992
    ....
  25. Regulation of Mitochondrial Nitric-oxide Synthase
    Cecilia Giulivi; Fiscal Year: 2003
    ..The production of this molecule was demonstrated by the oxidation of oxymyoglobin, electron spin resonance spectroscopy with the spin trapping technique, and L-citrulline production...
  26. STRUCTURE-FUNCTION STUDIES OF A SWITCHABLE ION CHANNEL
    FRANCOISE LEVINTHAL; Fiscal Year: 1991
    ..In all cases, extensive computations will be done in order to make theoretical estimates of the channel conductance and their changes and these will be compared with the observed values...
  27. REGULATION OF BROWN ADIPOSE TISSUE THERMOGENESIS
    EARL SHRAGO; Fiscal Year: 1990
    ....
  28. PHARMACOLOGICAL EFFECTS OF ETHER LIPID ANALOGS
    EDWARD MODEST; Fiscal Year: 1991
    ..the ability of the compounds to accelerate monocyte maturation, which may contribute to in vivo antitumor activity by increasing the number of mature tissue macrophages available for activation, will be examined...
  29. IMAGING OXYGEN--LOW FREQUENCY ELECTRON SPIN RESONANCE
    Howard Halpern; Fiscal Year: 1993
    ..It promises to provide answers to questions unanswered after decades of research...
  30. AGE, REACTIVE OXYGEN METABOLITES AND MYOCARDIAL INJURY
    John Baker; Fiscal Year: 1993
    ....
  31. BASIS OF OXYGEN-DEPENDENT NEUTROPHIL HOST DEFENSES
    Robert Clark; Fiscal Year: 1993
    ..In Project 2 electron spin resonance spectroscopy will be the key analytical tool for defining physical chemical and cellular factors that modulate ..
  32. ESR SPIN LABEL STUDIES OF FIBRONECTIN
    Ching San Lai; Fiscal Year: 1993
    ..This information should provide a basis for explaining important roles of fibronectin in cell attachment and migration, embryogenesis, wound healing, and oncogenic transformation...
  33. MECHANISM OF AUTOTROPHIC GROWTH WITH CO OR CO2 & H2
    Ganesh Kumar; Fiscal Year: 1993
    ..The properties of CO dehydrogenase from certain methanogenic bacteria will be tested for their ability to synthesize acetyl-CoA to determine whether they may use a pathway of acetyl-CoA synthesis similar to that of C. thermoaceticum...
  34. ROLE OF ETHANOL IN FREE RADICAL GENERATION
    Albert Sun; Fiscal Year: 1991
    ..The technique of electron spin resonance spectroscopy for the detection and identification of free radicals will be employed...
  35. MECHANISM OF AUTOTROPHIC GROWTH WITH CO OR CO2 AND H2
    Harland Wood; Fiscal Year: 1991
    ..The properties of CO dehydrogenase from certain methanogenic bacteria will be tested for their ability to synthesize acetyl-CoA to determine whether they may use a pathway of acetyl-CoA synthesis similar to that of C. thermoaceticum...
  36. LAMB PROTEIN TRANSPORT CHANNEL IN E. COLI OUTER MEMBRANE
    MARY LUCKEY; Fiscal Year: 1991
    ..In addition, characterization of the outer membrane of Gram negative bacteria may further our understanding of bacterial infection and antibiotic resistance among bacteria...
  37. PROTEIN/LIPID INTERACTIONS
    Robert Gennis; Fiscal Year: 2002
    ..The aa(3)-type oxidase must have a proton-conducting channel spanning the membrane. Among our targets will residues which are components of the channel. ..
  38. ACTIVATION AND TOXICITIES OF PHENOLIC COMPOUNDS
    Michael Trush; Fiscal Year: 1992
    ..In this regard, the investigators will (1) characterize by electron spin resonance spectroscopy the free radical intermediates, generated from BHT and its metabolites, (2) investigate the ..
  39. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 1999
    ..Colicin E1, a voltage-gated ion channel, will be investigated to probe molecular mechanisms of gating. ..
  40. TOPICAL IN-VIVO ELECTRON SPIN RESONANCE
    Lawrence Berliner; Fiscal Year: 1993
    ..monitoring tumour growth characteristics by spin label metabolism c. spin trapping studies of a swine global myocardial ischemia reperfusion model; d. nitroxide monitoring in the brain...
  41. SITE DIRECTED SPIN LABELING OF FEPA
    Jimmy Feix; Fiscal Year: 2002
    ..These studies may significantly advance understanding of the structure and dynamics of ligand-gated bacterial outer membrane receptors. ..
  42. Effects of Nitric Oxide in Sickle Cell Blood
    DANIEL KIM SHAPIRO; Fiscal Year: 2006
    ..Our studies will help us understand important issues related to NO's role in sickle cell disease and in the general population. ..
  43. MODERN ESR TECHNIQUES FOR BIOLOGICAL STUDIES
    Jack Freed; Fiscal Year: 2000
    ..New, more detailed models of the complex molecular dynamics of biomolecules will be incorporated into the analysis of the ESR experiments. ..
  44. Fas-Induced Apoptosis and Inflammatory Lung Injury
    Yon Rojanasakul; Fiscal Year: 2003
    ..We expect that the proposed studies will provide valuable new information on the molecular mechanisms of Fas induced apoptosis and its role in lung pathogenesis. ..
  45. Reactive Inflammatory Species in Heart Failure
    Pamela Lucchesi; Fiscal Year: 2006
    ..The proposed investigations are fundamentally important to the development of therapeutic strategies targeted to oxidant-induced injury and may have important implications in the treatment of HF. ..
  46. SOCIETY OF MAGNETIC RESEARCH IN MEDICINE
    LAWRENCE CROOKS; Fiscal Year: 1990
    ..Bringing together the finest researchers and the students of this rapidly evolving field will disseminate knowledge and enhance progress in important areas of medical research that bear on virtually every medical discipline...
  47. ELECTRON PARAMAGNETIC SPIN RESONANCE FACILITY
    John Raymond; Fiscal Year: 1999
    ..It will serve as a platform for interdisciplinary investigation and training, and will significantly enhance the competitiveness of MUSC investigators. ..
  48. Mechanisms of As-Induced Carcinogenesis
    Xianglin Shi; Fiscal Year: 2010
    ..The results obtained from this proposal will provide a fundamental understanding concerning the role of ROS in As-induced carcinogenesis. ..
  49. Development of EPR and NIR Oximetry for Diabetic Foot
    Mohammad Khan; Fiscal Year: 2007
    ..If the promise of these techniques is fulfilled, clinicians will have a new powerful tool for management of peripheral vascular disease, based on fundamental pathophysiology. [unreadable] [unreadable] [unreadable]..
  50. Monitoring Oxygen Repeatedly for Hypoxic Tumor Therapy
    Mohammad Khan; Fiscal Year: 2007
    ..If successful, this approach potentially could be immediately extended for clinical applications. [unreadable] [unreadable] [unreadable]..
  51. Oxygen Guided Hypofractionated Radiotherapy for Gliomas
    Nadeem Khan; Fiscal Year: 2010
    ....
  52. MECHANISMS OF CHAPERONE FUNCTION IN THE LENS
    Hassane McHaourab; Fiscal Year: 2008
    ..Reporter group spectroscopic approaches will be used to provide insight into the structural basis of recognition and binding. ..
  53. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 2008
    ..The last aim will provide direct data on the membrane topology of PGHS and the mechanism of its substrate presentation and product release. [unreadable] [unreadable]..
  54. Biomimetic Studies of NO-binding Respiratory Chain Hemes
    JAMES COLLMAN; Fiscal Year: 2008
    ..An emphasis will be placed on kinetic and mechanistic studies to elucidate the putitive reaction pathways of CcO and NOR. ..
  55. Mechanisms of Tumor Promotion Effects of B[a]PDE
    Chuanshu Huang; Fiscal Year: 2008
    ..Such agents could interfere with the signaling pathways leading to AP-1 activation. ..
  56. EPR Sample-cell-Resonator Design and Construction
    James Hyde; Fiscal Year: 2008
    ..Work proposed here will improve the quality of data obtained in these experiments. [unreadable] [unreadable] [unreadable]..
  57. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    TOMOKO NONE OHNISHI; Fiscal Year: 2010
    ..Using this new strategy, we found that both cluster N2 and flavin can generate superoxide. We will solidify this exciting finding and study its relevance to complex I function under physiological and pathological conditions. ..
  58. Functional active-site models of cytochrome c oxidase
    JAMES COLLMAN; Fiscal Year: 2009
    ..The role that the copper ion (CuB) and a phenolic group, in the amino acid tyrosine (Tyr244) play in the catalytic function of CcO are of particular interest. ..
  59. Structure-Function and Reacation Mechanism of eNOS
    Ah Lim Tsai; Fiscal Year: 2007
    ..The last Aim will provide understanding of the role of reductase and CaM in regulating the redox coupling with the oxygenase domain thus lead to an elucidation of the reaction of the reaction mechanism of the whole eNOS. ..
  60. UPGRADE OF VARIABLE FREQUENCY PULSED EPR FACILITY
    JOHN ENEMARK; Fiscal Year: 2005
    ....
  61. 3T Whole-Body MRI Scanner for Functional Imaging
    James Hyde; Fiscal Year: 2003
    ..It will have no clinical fee-for-service usage. The fMRI group at MCW is superbly positioned to provide leadership at a national level in the use of fMRI in medicine. ..
  62. STRUCTURAL MODELS FOR MOLYBDOENZYMES
    John H Enemark; Fiscal Year: 2011
    ..The rates of intramolecular electron transfer between the molybdenum and iron centers of SO will be investigated by flash photolysis and theoretical modeling. ..