circular dichroism

Summary

Summary: A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Top Publications

  1. ncbi How to study proteins by circular dichroism
    Sharon M Kelly
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochim Biophys Acta 1751:119-39. 2005
  2. ncbi Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases
    Lee Whitmore
    Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK
    Biopolymers 89:392-400. 2008
  3. pmc DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data
    Lee Whitmore
    Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK
    Nucleic Acids Res 32:W668-73. 2004
  4. pmc Circular dichroism and conformational polymorphism of DNA
    Jaroslav Kypr
    Institute of Biophysics, vvi Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Nucleic Acids Res 37:1713-25. 2009
  5. pmc Using circular dichroism spectra to estimate protein secondary structure
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, New Jersey 08854 8021, USA
    Nat Protoc 1:2876-90. 2006
  6. ncbi Circular dichroism and guanine quadruplexes
    Michaela Vorlíčková
    Institute of Biophysics, Academy of Sciences of the Czech Republic, v v i, Kralovopolska 135, CZ 612 65 Brno, Czech Republic
    Methods 57:64-75. 2012
  7. ncbi DICHROWEB: an interactive website for the analysis of protein secondary structure from circular dichroism spectra
    A Lobley
    Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK
    Bioinformatics 18:211-2. 2002
  8. ncbi Circular dichroism techniques: biomolecular and nanostructural analyses- a review
    Bijan Ranjbar
    Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
    Chem Biol Drug Des 74:101-20. 2009
  9. ncbi Analyzing protein circular dichroism spectra for accurate secondary structures
    W C Johnson
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331 7305, USA
    Proteins 35:307-12. 1999
  10. ncbi Conformational constraints for amyloid fibrillation: the importance of being unfolded
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Biochim Biophys Acta 1698:131-53. 2004

Detail Information

Publications306 found, 100 shown here

  1. ncbi How to study proteins by circular dichroism
    Sharon M Kelly
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biochim Biophys Acta 1751:119-39. 2005
    b>Circular dichroism (CD) is being increasingly recognised as a valuable technique for examining the structure of proteins in solution...
  2. ncbi Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases
    Lee Whitmore
    Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK
    Biopolymers 89:392-400. 2008
    b>Circular dichroism (CD) spectroscopy has been a valuable method for the analysis of protein secondary structures for many years...
  3. pmc DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data
    Lee Whitmore
    Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK
    Nucleic Acids Res 32:W668-73. 2004
    The DICHROWEB web server enables on-line analyses of circular dichroism (CD) spectroscopic data, providing calculated secondary structure content and graphical analyses comparing calculated structures and experimental data...
  4. pmc Circular dichroism and conformational polymorphism of DNA
    Jaroslav Kypr
    Institute of Biophysics, vvi Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Nucleic Acids Res 37:1713-25. 2009
    ..Due to its numerous advantages, CD spectroscopy significantly participated in all basic conformational findings on DNA...
  5. pmc Using circular dichroism spectra to estimate protein secondary structure
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, New Jersey 08854 8021, USA
    Nat Protoc 1:2876-90. 2006
    b>Circular dichroism (CD) is an excellent tool for rapid determination of the secondary structure and folding properties of proteins that have been obtained using recombinant techniques or purified from tissues...
  6. ncbi Circular dichroism and guanine quadruplexes
    Michaela Vorlíčková
    Institute of Biophysics, Academy of Sciences of the Czech Republic, v v i, Kralovopolska 135, CZ 612 65 Brno, Czech Republic
    Methods 57:64-75. 2012
    b>Circular dichroism (CD) is remarkably sensitive to the conformational states of nucleic acids; therefore, CD spectroscopy has been used to study most features of DNA and RNA structures...
  7. ncbi DICHROWEB: an interactive website for the analysis of protein secondary structure from circular dichroism spectra
    A Lobley
    Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK
    Bioinformatics 18:211-2. 2002
    A user-friendly website for the analysis of protein secondary structures from Circular Dichroism (CD) and Synchrotron Radiation Circular Dichroism (SRCD) spectra has been created.
  8. ncbi Circular dichroism techniques: biomolecular and nanostructural analyses- a review
    Bijan Ranjbar
    Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
    Chem Biol Drug Des 74:101-20. 2009
    This paper reviews the best known techniques using circular dichroism spectroscopy such as conventional circular dichroism (i.e...
  9. ncbi Analyzing protein circular dichroism spectra for accurate secondary structures
    W C Johnson
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331 7305, USA
    Proteins 35:307-12. 1999
    We have developed an algorithm to analyze the circular dichroism of proteins for secondary structure. Its hallmark is tremendous flexibility in creating the basis set, and it also combines the ideas of many previous workers...
  10. ncbi Conformational constraints for amyloid fibrillation: the importance of being unfolded
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Biochim Biophys Acta 1698:131-53. 2004
    ..The inherent flexibility of such an intermediate is essential in allowing the conformational rearrangements necessary to form the core cross-beta structure of the amyloid fibril...
  11. pmc Structure of the human telomere in K+ solution: a stable basket-type G-quadruplex with only two G-tetrad layers
    Kah Wai Lim
    School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore
    J Am Chem Soc 131:4301-9. 2009
    ..This novel structure highlights the conformational heterogeneity of human telomeric DNA. It establishes a new folding principle for G-quadruplexes and suggests new loop sequences and structures for targeting in human telomeric DNA...
  12. ncbi Circular dichroism of quadruplex structures
    Antonio Randazzo
    Dipartimento di Chimica Farmaceutica e Tossicologica, Universita degli Studi di Napoli Federico II, via D Montesano 49, 80131, Napoli, Italy
    Top Curr Chem 330:67-86. 2013
    b>Circular dichroism (CD) is a widespread technique for studying the polymorphism of G-quadruplexes...
  13. ncbi A non-empirical chromophoric interpretation of CD spectra of DNA G-quadruplex structures
    Stefano Masiero
    Alma Mater Studiorum Universita di Bologna, Dipartimento di Chimica Organica A Mangini, Via San Giacomo 11, I 40126, Bologna, Italy
    Org Biomol Chem 8:2683-92. 2010
    ....
  14. pmc How long is too long? Effects of loop size on G-quadruplex stability
    Aurore Guédin
    INSERM, U869, European Institute of Chemistry and Biology, Bordeaux 2 University, 2 rue Robert Escarpit, Pessac F 33607, France
    Nucleic Acids Res 38:7858-68. 2010
    ..This study will therefore extend the sequence repertoire of quadruplex-prone sequences, arguing for a modification of the widely used consensus (maximal loop size of 7 bases)...
  15. pmc Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    Nat Protoc 1:2527-35. 2006
    b>Circular dichroism (CD) is an excellent spectroscopic technique for following the unfolding and folding of proteins as a function of temperature...
  16. pmc An intramolecular G-quadruplex structure with mixed parallel/antiparallel G-strands formed in the human BCL-2 promoter region in solution
    Jixun Dai
    College of Pharmacy, University of Arizona, Tucson, 85721, USA
    J Am Chem Soc 128:1096-8. 2006
    ..This predominant bcl-2 G-quadruplex represents an attractive novel target for the design of new anticancer drugs that specifically modulate bcl-2 gene expression...
  17. pmc K2D2: estimation of protein secondary structure from circular dichroism spectra
    Carolina Perez-Iratxeta
    Ontario Genomics Innovation Centre, Ottawa Health Research Institute, 501 Smyth, Ottawa, ON, K1H 8L6, Canada
    BMC Struct Biol 8:25. 2008
    b>Circular dichroism spectroscopy is a widely used technique to analyze the secondary structure of proteins in solution...
  18. ncbi The use of circular dichroism in the investigation of protein structure and function
    S M Kelly
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Scotland, G12 8QQ, UK
    Curr Protein Pept Sci 1:349-84. 2000
    b>Circular Dichroism (CD) relies on the differential absorption of left and right circularly polarised radiation by chromophores which either possess intrinsic chirality or are placed in chiral environments...
  19. pmc Pollutant-induced modulation in conformation and β-lactamase activity of human serum albumin
    Ejaz Ahmad
    Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India
    PLoS ONE 7:e38372. 2012
    ..human serum albumin (HSA) induced by the pollutants 1-naphthol, 2-naphthol and 8-quinolinol were analyzed by circular dichroism, fluorescence spectroscopy and dynamic light scattering...
  20. ncbi Circular dichroism spectroscopy of DNA: from duplexes to quadruplexes
    Michaela Vorlíčková
    Department of CD Spectroscopy of Nucleic Acids, Institute of Biophysics, Academy of Sciences of the Czech Republic, vvi, Brno, Czech Republic
    Chirality 24:691-8. 2012
    ..The major factor enabling the specific recognition is structure. Circular dichroism (CD) spectroscopy is very useful to study secondary structures of nucleic acids, in general, and DNA, in ..
  21. ncbi Effect of familial Parkinson's disease point mutations A30P and A53T on the structural properties, aggregation, and fibrillation of human alpha-synuclein
    J Li
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 40:11604-13. 2001
    ..A variety of biophysical methods, including far-UV circular dichroism, FTIR, small-angle X-ray scattering, and light scattering, were employed...
  22. ncbi Protein disorder prediction: implications for structural proteomics
    Rune Linding
    EMBL, Biocomputing Unit, Meyerhofstr 1, D 69117 Heidelberg, Germany
    Structure 11:1453-9. 2003
    ..The tool is freely available via a web interface (http://dis.embl.de) and can be downloaded for use in large-scale studies...
  23. ncbi Methods for investigating G-quadruplex DNA/ligand interactions
    Pierre Murat
    Department of Chemistry, The University of Cambridge, Cambridge CB2 1EW, UK
    Chem Soc Rev 40:5293-307. 2011
    ..This tutorial review provides an overview of G-quadruplex ligands and biophysical techniques available to monitor such interactions...
  24. ncbi A reference database for circular dichroism spectroscopy covering fold and secondary structure space
    Jonathan G Lees
    Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK
    Bioinformatics 22:1955-62. 2006
    b>Circular Dichroism (CD) spectroscopy is a long-established technique for studying protein secondary structures in solution...
  25. pmc Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution
    Attila Ambrus
    College of Pharmacy, The University of Arizona, 1703 E Mabel St, Tucson, AZ 85721, USA
    Nucleic Acids Res 34:2723-35. 2006
    ....
  26. ncbi The effects of arginine on refolding of aggregated proteins: not facilitate refolding, but suppress aggregation
    Tsutomu Arakawa
    Alliance Protein Laboratories, Thousand Oaks, CA 91360, USA
    Biochem Biophys Res Commun 304:148-52. 2003
    ..It does increase reversibility of thermal melting and reduce aggregation under thermal stress. The observations suggest that arginine may not facilitate refolding, but may suppress aggregation of the proteins during refolding...
  27. ncbi Both familial Parkinson's disease mutations accelerate alpha-synuclein aggregation
    L Narhi
    Amgen, Inc, Thousand Oaks, California 91320 1789, USA
    J Biol Chem 274:9843-6. 1999
    ..These data suggest that the formation of alpha-synuclein aggregates could be a critical step in PD pathogenesis, which is accelerated by the PD-linked mutations...
  28. pmc Energetics and kinetics of a conformational switch in G-quadruplex DNA
    Robert D Gray
    James Graham Brown Cancer Center, University of Louisville, 529 South Jackson Street, Louisville, Kentucky 40202, USA
    J Phys Chem B 113:2676-83. 2009
    b>Circular dichroism and differential scanning calorimetry were used to determine the energetics of the conformational switch of the human telomere quadruplex formed by the sequence d[AGGG(TTAGGG)3] between the sodium basket form and the ..
  29. pmc Sequence occurrence and structural uniqueness of a G-quadruplex in the human c-kit promoter
    Alan K Todd
    Cancer Research UK Biomolecular Structure Group, The School of Pharmacy, University of London, 29 39 Brunswick Square, London WC1N 1AX, UK
    Nucleic Acids Res 35:5799-808. 2007
    ..The c-kit87 quadruplex thus fulfils a fundamental criterion of a 'good' drug target, in that it possesses distinctive three-dimensional structural features that are only present in at most a handful of other genes...
  30. ncbi Structural analysis of DNA interactions with biogenic polyamines and cobalt(III)hexamine studied by Fourier transform infrared and capillary electrophoresis
    Amin Ahmed Ouameur
    Department of Chemistry Biology, University of Quebec at Trois Rivieres, C P 500, Trois Rivieres, Quebec G9A 5H7, Canada
    J Biol Chem 279:42041-54. 2004
    ..calf-thymus DNA were investigated using affinity capillary electrophoresis, Fourier transform infrared, and circular dichroism spectroscopic methods...
  31. ncbi ILPR G-quadruplexes formed in seconds demonstrate high mechanical stabilities
    Zhongbo Yu
    Department of Chemistry, Kent State University, Kent, Ohio 44242, USA
    J Am Chem Soc 131:1876-82. 2009
    ..Using mechanical unfolding in a laser tweezers instrument and circular dichroism (CD) spectroscopy, we provide compelling evidence that highly stable parallel and antiparallel G quadruplex ..
  32. ncbi CDtool-an integrated software package for circular dichroism spectroscopic data processing, analysis, and archiving
    J G Lees
    Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK
    Anal Biochem 332:285-9. 2004
    CDtool is a software package written to facilitate circular dichroism (CD) spectroscopic studies on both conventional lab-based instruments and synchrotron beamlines...
  33. ncbi HIV-integrase aptamer folds into a parallel quadruplex: a thermodynamic study
    Sean Kelley
    Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    Biophys Chem 155:82-8. 2011
    ..Here we probed an apparent discrepancy between the NMR structure and the quadruplex topology suggested by circular dichroism (CD)...
  34. ncbi Intermediates and kinetic traps in the folding of a large ribozyme revealed by circular dichroism and UV absorbance spectroscopies and catalytic activity
    T Pan
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA
    Nat Struct Biol 4:931-8. 1997
    The folding thermodynamics and kinetics for the ribozyme from Bacillus subtilis RNase P are analyzed using circular dichroism and UV absorbance spectroscopies and catalytic activity...
  35. pmc Valley-selective circular dichroism of monolayer molybdenum disulphide
    Ting Cao
    International Center for Quantum Materials, Peking University, Beijing 100871, China
    Nat Commun 3:887. 2012
    ..is an ideal material for valleytronics, for which valley polarization is achievable via valley-selective circular dichroism arising from its unique symmetry...
  36. ncbi Binding properties of human telomeric quadruplex multimers: a new route for drug design
    Annunziata Cummaro
    Department of Chemistry P Corradini, University of Naples Federico II, Via Cintia, 4, I 80126 Napoli, Italy
    Biochimie 93:1392-400. 2011
    ..The existence of quadruplex-quadruplex interfaces in the full length telomeric overhang may provide an advantageous factor in drug design to enhance both affinity and selectivity for DNA telomeric quadruplexes...
  37. ncbi A new method for purification of recombinant human alpha-synuclein in Escherichia coli
    Chunjuan Huang
    The National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing
    Protein Expr Purif 42:173-7. 2005
    ..coli strains commonly used the new protocol is much more convenient, faster, and cheaper compared to the current methods established since 1994. About 80 mg AS with 95% purity can be regularly prepared from a 1L culture in 3 days...
  38. ncbi Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins
    H Kaspar Binz
    Biochemisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Mol Biol 332:489-503. 2003
    ..Moreover, the favorable biophysical properties and the modularity of the AR fold may account, partly, for the abundance of natural AR proteins...
  39. ncbi Crystal structure of a hypoallergenic isoform of the major birch pollen allergen Bet v 1 and its likely biological function as a plant steroid carrier
    Zora Markovic-Housley
    Department of Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    J Mol Biol 325:123-33. 2003
    ..The role of BRs transport in PR-10 proteins may be of crucial importance in the plant defense response to pathological situations as well as in growth and development...
  40. pmc Formation of pseudosymmetrical G-quadruplex and i-motif structures in the proximal promoter region of the RET oncogene
    Kexiao Guo
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    J Am Chem Soc 129:10220-8. 2007
    ..Moreover, comparative circular dichroism (CD) and DMS footprinting studies have revealed that the 3'-G-quadruplex structure is a parallel-type ..
  41. pmc Structure of a two-G-tetrad intramolecular G-quadruplex formed by a variant human telomeric sequence in K+ solution: insights into the interconversion of human telomeric G-quadruplex structures
    Zhenjiang Zhang
    College of Pharmacy, The University of Arizona, Tucson, AZ 85721, USA
    Nucleic Acids Res 38:1009-21. 2010
    ....
  42. pmc Monitoring protein aggregation during thermal unfolding in circular dichroism experiments
    Sangeeta Benjwal
    Department of Physiology and Biophysics, Boston University School of Medicine, W329, 715 Albany Street, Boston, MA 02118, USA
    Protein Sci 15:635-9. 2006
    ..during thermal folding/ unfolding transition by recording turbidity or 90 degrees light scattering data in circular dichroism (CD) spectroscopic experiments...
  43. pmc G4 resolvase 1 tightly binds and unwinds unimolecular G4-DNA
    Banabihari Giri
    Department of Cancer Biology and the Comprehensive Cancer Center of Wake Forest University School of Medicine, Winston Salem, USA
    Nucleic Acids Res 39:7161-78. 2011
    ..This report provides evidence that G4R1/RHAU tightly binds and unwinds unimolecular G4-DNA structures...
  44. ncbi Low concentrations of sodium dodecyl sulfate induce the extension of beta 2-microglobulin-related amyloid fibrils at a neutral pH
    Suguru Yamamoto
    Division of Molecular Pathology, Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910 1193, Japan
    Biochemistry 43:11075-82. 2004
    ..Using fluorescence spectroscopy with thioflavin T, circular dichroism spectroscopy, and electron microscopy, we here show that at low concentrations, sodium dodecyl sulfate (SDS) ..
  45. ncbi A hydrogen bond surrogate approach for stabilization of short peptide sequences in alpha-helical conformation
    Anupam Patgiri
    Department of Chemistry, New York University, New York, New York 10003, USA
    Acc Chem Res 41:1289-300. 2008
    ..Extensive conformational analysis of HBS alpha-helices with 2D NMR, circular dichroism spectroscopies and X-ray crystallography confirms the alpha-helical structure in these compounds...
  46. ncbi Pathway complexity of prion protein assembly into amyloid
    Ilia V Baskakov
    Institute for Neurodegenerative Diseases, University of California, San Francisco, California 94143, USA
    J Biol Chem 277:21140-8. 2002
    ....
  47. ncbi Characterization of the in situ structural and interfacial properties of the cationic hydrophobic heteropolypeptide, KL4, in lung surfactant bilayer and monolayer models at the air-water interface: implications for pulmonary surfactant delivery
    Heidi M Mansour
    Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin Madison, Madison, Wisconsin 53705, USA
    Mol Pharm 5:681-95. 2008
    ..In situ surface circular dichroism spectroscopy, using a method first reported by Damodaran (Damodaran, S. Anal. Bioanal. Chem...
  48. ncbi Interaction of (-)-epigallocatechin-3-gallate with human serum albumin: fluorescence, fourier transform infrared, circular dichroism, and docking studies
    Tushar Kanti Maiti
    Department of Chemistry, Indian Institute of Technology, Kharagpur, India
    Proteins 64:355-62. 2006
    ..of EGCG with human serum albumin (HSA) has been investigated for the first time by using fluorescence, circular dichroism (CD), Fourier transform infrared (FTIR) spectroscopy, and protein-ligand docking...
  49. pmc Investigation of spectral conversion of d(TTAGGG)4 and d(TTAGGG)13 upon potassium titration by a G-quadruplex recognizer BMVC molecule
    Cheng Chung Chang
    Institute of Atomic and Molecular Sciences, Academia Sinica, PO Box 23 166, Taipei, 106, Taiwan, Republic of China
    Nucleic Acids Res 35:2846-60. 2007
    ..The studies of circular dichroism, induced circular dichroism, spectral titration and gel competition have allowed us to determine the binding ..
  50. ncbi Conformational behavior and aggregation of alpha-synuclein in organic solvents: modeling the effects of membranes
    Larissa A Munishkina
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 42:2720-30. 2003
    ..At least to some extent, these conformational effects mimic those observed in the presence of phospholipid vesicles, and can explain some of the observed effects of membranes on alpha-synuclein fibrillation...
  51. pmc Protein hnRNP A1 and its derivative Up1 unfold quadruplex DNA in the human KRAS promoter: implications for transcription
    Manikandan Paramasivam
    Department of Biomedical Science and Technology, School of Medicine, Udine, Italy
    Nucleic Acids Res 37:2841-53. 2009
    ..When quadruplex 32R is stabilized by TMPyP4, A1/Up1 brings about only a partial destabilization of the G4-DNA structure. The possible role played by hnRNP A1 in the mechanism of KRAS transcription is discussed...
  52. ncbi Circular dichroism spectrum of peptides in the poly(Pro)II conformation
    Robert W Woody
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Am Chem Soc 131:8234-45. 2009
    ..b>Circular dichroism (CD) is one of the most useful methods for detecting and characterizing P(II)...
  53. ncbi Resveratrol inhibits beta-amyloid oligomeric cytotoxicity but does not prevent oligomer formation
    Ying Feng
    Tsinghua University School of Medicine, Haidian District, Beijing 100084, China
    Neurotoxicology 30:986-95. 2009
    ..using fluorescence spectroscopic analysis with thioflavin T (ThT), transmission electron microscope (TEM), circular dichroism (CD) and MTT assay...
  54. ncbi Shedding light on the interaction between TMPyP4 and human telomeric quadruplexes
    Luigi Martino
    Dipartimento di Chimica P Corradini, Universita degli Studi di Napoli Federico II, Via Cintia, I 80126, Napoli, Italy
    J Phys Chem B 113:14779-86. 2009
    ..of human telomeric DNA, with 5'- or 3'-flanking bases, using isothermal titration calorimetry and circular dichroism. The results clearly indicate that all of these G-quadruplexes are able to bind up to four TMPyP4 molecules...
  55. ncbi Plasmonic circular dichroism of chiral metal nanoparticle assemblies
    Zhiyuan Fan
    Department of Physics and Astronomy, Ohio University, Athens, Ohio 45701, USA
    Nano Lett 10:2580-7. 2010
    ..In our model, the circular dichroism (CD) signal comes from the Coulomb interaction between NPs...
  56. ncbi Design, production and molecular structure of a new family of artificial alpha-helicoidal repeat proteins (αRep) based on thermostable HEAT-like repeats
    Agathe Urvoas
    Institut de Biochimie Moléculaire et Cellulaire IBBMC, Univ Paris Sud, UMR 8619, Orsay, F 91405 Orsay, France
    J Mol Biol 404:307-27. 2010
    ..b>Circular dichroism and differential scanning calorimetry experiments showed that all these proteins are folded cooperatively ..
  57. pmc Non-esterified fatty acids generate distinct low-molecular weight amyloid-β (Aβ42) oligomers along pathway different from fibril formation
    Amit Kumar
    Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi, United States of America
    PLoS ONE 6:e18759. 2011
    ....
  58. ncbi In vivo aggregation of a single enzyme limits growth of Escherichia coli at elevated temperatures
    Eyal Gur
    Department of Molecular Microbiology and Biotechnology, The George S Wise Faculty of Life Sciences, Tel Aviv University, Israel
    Mol Microbiol 46:1391-7. 2002
    ..Moreover, reducing the in vivo level of HTS aggregation enables growth at non-permissive temperatures. This is the first demonstration of the physiological role of aggregation of a specific protein in the growth of wild-type bacteria...
  59. ncbi Effect of Leucine and Lysine substitution on the antimicrobial activity and evaluation of the mechanism of the HPA3NT3 analog peptide
    Ramamourthy Gopal
    Research Center for Proteineous Materials RCPM, Chosun University, Gwangju, Korea
    J Pept Sci 15:589-94. 2009
    ..The HPA3NT3-analog also induced less hemolysis (4.2%) than the HPA3NT3 peptide (71%) at 200 microM. Circular dichroism (CD) spectra revealed that the HPA3NT3-analog peptide had an unordered structure in buffer and egg yolk L-2-..
  60. ncbi Probing the interaction of human serum albumin with ciprofloxacin in the presence of silver nanoparticles of three sizes: multispectroscopic and ζ potential investigation
    Hediyeh Iranfar
    Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
    J Phys Chem B 116:1951-64. 2012
    ..For this purpose fluorescence spectroscopy, circular dichroism, UV-vis spectroscopy, and ζ potential techniques were employed under physiological conditions...
  61. pmc Insight into the assembly properties and functional organisation of the magnetotactic bacterial actin-like homolog, MamK
    Sanjiv Sonkaria
    Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany
    PLoS ONE 7:e34189. 2012
    ..b>Circular dichroism and structural homology modelling showed that MamK adopts a protein fold that is consistent with the '..
  62. pmc Interaction of virstatin with human serum albumin: spectroscopic analysis and molecular modeling
    Tanaya Chatterjee
    Department of Biochemistry, Bose Institute, Kolkata, India
    PLoS ONE 7:e37468. 2012
    ..The conformational changes of HSA due to the interaction with the drug were investigated from circular dichroism (CD) and Fourier Transform Infrared (FTIR) spectroscopy...
  63. pmc The role of large-scale motions in catalysis by dihydrofolate reductase
    E Joel Loveridge
    School of Chemistry, Cardiff University, Park Place, Cardiff CF10 3AT, United Kingdom
    J Am Chem Soc 133:20561-70. 2011
    ....
  64. ncbi Three-dimensional plasmonic chiral tetramers assembled by DNA origami
    Xibo Shen
    National Center for Nanoscience and Technology, No 11 Beiyitiao, Zhongguancun, Beijing 100190, China
    Nano Lett 13:2128-33. 2013
    ..For a long time, optical circular dichroism has been exclusively the terrain of natural chiral molecules, which exhibit optical activity mainly in the ..
  65. ncbi DNA converts cellular prion protein into the beta-sheet conformation and inhibits prion peptide aggregation
    Y Cordeiro
    Programa de Biologia Estrutural, , , Universidade Federal do Rio de Janeiro, Rio de Janeiro RJ 21941-590, Brazil
    J Biol Chem 276:49400-9. 2001
    ..0) completely inhibit aggregation of prion peptides, as measured by light scattering, fluorescence, and circular dichroism spectroscopy...
  66. pmc Structural, stability, dynamic and binding properties of the ALS-causing T46I mutant of the hVAPB MSP domain as revealed by NMR and MD simulations
    Shixiong Lua
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, Singapore
    PLoS ONE 6:e27072. 2011
    ....
  67. ncbi A conserved spider silk domain acts as a molecular switch that controls fibre assembly
    Franz Hagn
    Center for Integrated Protein Science CIPSM, Technische Universitat Munchen, 85747 Garching, Germany
    Nature 465:239-42. 2010
    ....
  68. ncbi Secondary and tertiary structure formation of the beta-barrel membrane protein OmpA is synchronized and depends on membrane thickness
    Jörg H Kleinschmidt
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, VA 22908 0736, USA
    J Mol Biol 324:319-30. 2002
    ..We have used fluorescence spectroscopy, circular dichroism spectroscopy, and gel electrophoresis to investigate basic mechanistic principles of structure formation in ..
  69. pmc Structures of 2-acetylaminofluorene modified DNA revisited: insight into conformational heterogeneity
    Satyakam Patnaik
    Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, USA
    Chem Res Toxicol 23:1650-2. 2010
    ..The S/B/W-conformational heterogeneities were found to be sensitive to the nature of the flanking DNA sequence contexts and pH...
  70. pmc Separating instability from aggregation propensity in γS-crystallin variants
    William D Brubaker
    Department of Molecular Biology and Biochemistry, University of California, Irvine, USA
    Biophys J 100:498-506. 2011
    Molecular dynamics (MD) simulations, circular dichroism (CD), and dynamic light scattering (DLS) measurements were used to investigate the aggregation propensity of the eye-lens protein γS-crystallin...
  71. pmc Structure of a bacterial voltage-gated sodium channel pore reveals mechanisms of opening and closing
    Emily C McCusker
    Department of Crystallography, Institute of Structural and Molecular Biology, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK
    Nat Commun 3:1102. 2012
    ..Comparison of the open and closed structures provides new insight into the features of the functional states present in the activation cycles of sodium channels and the mechanism of channel opening and closing...
  72. ncbi Fluorescence intercalator displacement assay for screening G4 ligands towards a variety of G-quadruplex structures
    Phong Lan Thao Tran
    University of Bordeaux, ARNA Laboratory, Bordeaux, France
    Biochimie 93:1288-96. 2011
    ..Most compounds showed a good G4 vs duplex selectivity but exhibited little, if any, specificity for one quadruplex sequence over the others. The quest for the "perfect" specific G4 ligand is not over yet!..
  73. ncbi Induction of G-quadruplex DNA structure by Zn(II) 5,10,15,20-tetrakis(N-methyl-4-pyridyl)porphyrin
    Amlan J Bhattacharjee
    Department of Chemistry and Biochemistry, Swarthmore College, 500 College Ave, Swarthmore, PA 19081, USA
    Biochimie 93:1297-309. 2011
    ..of facilitating GQ folding of d(TAGGG)(2) from single stranded, or binding to preformed GQ, using UV-vis and circular dichroism (CD) spectroscopies...
  74. pmc Characterization of two soybean (Glycine max L.) LEA IV proteins by circular dichroism and Fourier transform infrared spectrometry
    Ming der Shih
    Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, ROC
    Plant Cell Physiol 51:395-407. 2010
    ..report on the characterization of two members of soybean LEA IV proteins, basic GmPM1 and acidic GmPM28, by circular dichroism and Fourier transform infrared spectroscopy...
  75. pmc Hydrophobic core mutations associated with cataract development in mice destabilize human gammaD-crystallin
    Kate L Moreau
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 284:33285-95. 2009
    ..These results support models of cataract formation in which generation of partially unfolded species are precursors to the aggregated cataractous states responsible for light scattering...
  76. ncbi LD spectroscopy of natural and synthetic biomaterials
    Matthew R Hicks
    Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
    Chem Soc Rev 39:3380-93. 2010
    ....
  77. ncbi Biflavonoids are superior to monoflavonoids in inhibiting amyloid-β toxicity and fibrillogenesis via accumulation of nontoxic oligomer-like structures
    Arjun Thapa
    Department of Biomaterials Engineering, Chosun University, Gwanju 501 759, Republic of Korea
    Biochemistry 50:2445-55. 2011
    ..Altogether, our data clearly show that biflavonoids, possibly because of the possession of two Aβ binders separated by an appropriate size linker, are likely to be promising therapeutics for suppressing Aβ toxicity...
  78. ncbi More stable structure of wheat germ lipase at low pH than its native state
    Ejaz Ahmad
    Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
    Biochimie 92:885-93. 2010
    ..Conformational changes occurring in the protein with varying pH, were monitored by circular dichroism (CD) spectroscopy, fluorescence emission spectroscopy, binding of hydrophobic dye, 1-anilino 8-..
  79. ncbi Monitoring the temperature unfolding of G-quadruplexes by UV and circular dichroism spectroscopies and calorimetry techniques
    Chris M Olsen
    Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE, USA
    Methods Mol Biol 608:147-58. 2010
    ..of d(G(2)T(2)G(2)TGTG(2)T(2)G(2)), G2, and d(G(3)T(2)G(3)TGTG(3)T(2)G(3)), G3 by a combination of UV and circular dichroism (CD) spectroscopies, and differential scanning calorimetry (DSC)...
  80. pmc Synchrotron radiation circular dichroism spectroscopy-defined structure of the C-terminal domain of NaChBac and its role in channel assembly
    Andrew M Powl
    Department of Crystallography, Institute of Structural and Molecular Biology, Birkbeck College, University of London, London WC1E 7HX, United Kingdom
    Proc Natl Acad Sci U S A 107:14064-9. 2010
    ..In this study we show that the highly sensitive method of synchrotron radiation circular dichroism (SRCD) spectroscopy can be used as a powerful tool to investigate the structure of the extramembranous C-..
  81. ncbi Circular dichroism spectra and electrophoretic mobility shift assays show that human replication protein A binds and melts intramolecular G-quadruplex structures
    Jun Huei Fan
    Department of Molecular and Cell Biology, Mail Stop FO31, The University of Texas at Dallas, 800 West Campbell Road, Richardson, Texas 75080, USA
    Biochemistry 48:1099-111. 2009
    ..In this study, circular dichroism (CD) and electrophoretic mobility shift assay (EMSA) experiments were used to show that hRPA can bind and ..
  82. ncbi Structure of propeller-type parallel-stranded RNA G-quadruplexes, formed by human telomeric RNA sequences in K+ solution
    Herry Martadinata
    Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371
    J Am Chem Soc 131:2570-8. 2009
    ..Based on these findings we proposed how higher-order structures might be formed by long telomeric RNA...
  83. ncbi General peroxidase activity of G-quadruplex-hemin complexes and its application in ligand screening
    Xiaohong Cheng
    Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
    Biochemistry 48:7817-23. 2009
    ..This peroxidase activity has also been demonstrated to be applicable for screening G-quadruplex ligands (potential anticancer reagents) using colorimetric and visual detection strategies...
  84. pmc Structure and function of the b/HLH/Z domain of USF
    A R Ferré-D'amaré
    Laboratories of Molecular Biophysics, Rockefeller University, New York, NY 10021
    EMBO J 13:180-9. 1994
    ..The ability to bind simultaneously to two independent sites suggests a role in DNA looping for the b/HLH/Z and Myc-related families of eukaryotic transcription factors...
  85. pmc Influence of flanking sequence context on the conformational flexibility of aminofluorene-modified dG adduct in dA mismatch DNA duplexes
    Nidhi Jain
    Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA
    Nucleic Acids Res 37:1628-37. 2009
    ..12-mer NG*N/NAN dA mismatch duplexes (G* = FAF, N = G, A, C, T) exhibited strongly positive induced circular dichroism in the 290-360 nm range (ICD(290-360 nm)), which supports the W conformation...
  86. ncbi c-di-GMP displays a monovalent metal ion-dependent polymorphism
    Zhaoying Zhang
    Department of Chemistry and Chemical Biology, 610 Taylor Road, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    J Am Chem Soc 126:16700-1. 2004
    ..The ability of this rigid molecule to adopt different alignments may be important to its biological role...
  87. ncbi Intrinsic disorder and autonomous domain function in the multifunctional nuclear protein, MeCP2
    Valerie H Adams
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 282:15057-64. 2007
    ..These studies also establish an experimental paradigm for characterizing the tertiary structures of other highly disordered proteins...
  88. pmc A small molecule that disrupts G-quadruplex DNA structure and enhances gene expression
    Zoë A E Waller
    The University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK
    J Am Chem Soc 131:12628-33. 2009
    ..These observations provide further evidence to suggest that G-quadruplex forming sequence motifs play a role that relates to transcription...
  89. ncbi Structural independence of the two EF-hand domains of caltractin
    Sudha Veeraraghavan
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 277:28564-71. 2002
    ..Thus, the C-terminal domain appears to also be capable of sensing Ca2+ signals but is activated by the binding of a single ion...
  90. pmc The i-motif in the bcl-2 P1 promoter forms an unexpectedly stable structure with a unique 8:5:7 loop folding pattern
    Samantha Kendrick
    Arizona Cancer Center, 1515 North Campbell Avenue, Tucson, Arizona 85724, USA
    J Am Chem Soc 131:17667-76. 2009
    ..Furthermore, the two adjacent large lateral loops in the i-motif structure provide an unexpected opportunity for protein and small molecule recognition...
  91. ncbi Quaternary ammonium zinc phthalocyanine: inhibiting telomerase by stabilizing G quadruplexes and inducing G-quadruplex structure transition and formation
    Lige Ren
    College of Chemistry and Molecular Sciences, Wuhan University, Hongshan, Luo Jia Shan, Hubei, Wuhan 430072, P R China
    Chembiochem 8:775-80. 2007
    ..More importantly, ZnPc was found to induce parallel structure formation in cation-deficient conditions. The stability of the induced structure was determined with CD melting assays...
  92. ncbi Reactivation of the p53 tumor suppressor pathway by a stapled p53 peptide
    Federico Bernal
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    J Am Chem Soc 129:2456-7. 2007
  93. ncbi Self-assembly of spider silk proteins is controlled by a pH-sensitive relay
    Glareh Askarieh
    Department of Chemistry, Oslo University, 1033 Blindern, 0315 Oslo, Norway
    Nature 465:236-8. 2010
    ....
  94. ncbi Evidence for the binding mode of porphyrins to G-quadruplex DNA
    Chunying Wei
    Institute of Molecular Science, Shanxi University, Taiyuan, 030006, China
    Phys Chem Chem Phys 11:4025-32. 2009
    ..58 x 10(6) and 1.24 x 10(7) M(-1) for parallel-stranded and antiparallel/parallel hybrid structures of AG(3)(T(2)AG(3))(3) G-quadruplex, respectively...
  95. ncbi G-quadruplex formation by human telomeric repeats-containing RNA in Na+ solution
    Yan Xu
    Research Center for Advanced Science and Technology, The University of Tokyo, 4 6 1 Komaba, Meguro ku, Tokyo 153 8904, Japan
    J Am Chem Soc 130:11179-84. 2008
    ..In the current study, we used a combination of NMR, circular dichroism (CD), matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOFMS), and gel ..
  96. pmc EGCG remodels mature alpha-synuclein and amyloid-beta fibrils and reduces cellular toxicity
    Jan Bieschke
    Max Delbrueck Center for Molecular Medicine, Robert Roessle Strasse 10, 13125 Berlin Buch, Germany
    Proc Natl Acad Sci U S A 107:7710-5. 2010
    ..These findings suggest that EGCG is a potent remodeling agent of mature amyloid fibrils...
  97. ncbi A novel quaternary structure of the dimeric alpha-crystallin domain with chaperone-like activity
    I K Feil
    European Molecular Biology Laboratory EMBL, EMBL Hamburg Outstation, Notkestrasse 85, D 22603 Hamburg, Germany
    J Biol Chem 276:12024-9. 2001
    ..This flexibility may be important for biological activity and for the formation of alphaB-crystallin complexes of variable sizes and compositions...
  98. ncbi A single-point mutation converts the highly amyloidogenic human islet amyloid polypeptide into a potent fibrillization inhibitor
    Andisheh Abedini
    Department of Chemistry, State University of New York at Stony Brook, Stony Brook, New York 11794 3400, USA
    J Am Chem Soc 129:11300-1. 2007
  99. ncbi Conserved residue Lys574 in the cavity of HIV-1 Gp41 coiled-coil domain is critical for six-helix bundle stability and virus entry
    Yuxian He
    Lindsley F Kimball Research Institute, New York Blood Center, New York, New York 10021, USA
    J Biol Chem 282:25631-9. 2007
    ..Lys574 substitutions severely impaired the stability and conformation of 6-HBs as detected by circular dichroism spectroscopy, native polyacrylamide gel electrophoresis, and enzyme-linked immunosorbent assay...
  100. pmc The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil
    Rebecca Conners
    Department of Biochemistry, University of Bristol, Bristol, UK
    EMBO J 27:1779-89. 2008
    ..This explains how UspA1 can engage CEACAM1 at a site far distant from its head group, permitting closer proximity of the respective cell surfaces during infection...
  101. pmc Amyloid-beta peptide assembly: a critical step in fibrillogenesis and membrane disruption
    C M Yip
    Institute for Biomaterials and Biomedical Engineering and Centre for Studies in Molecular Imaging, University of Toronto, Toronto, Ontario M5S 3G9 Canada
    Biophys J 80:1359-71. 2001
    ..We describe the results of a combined circular dichroism spectroscopy, electron microscopy, and in situ tapping mode atomic force microscopy (TMAFM) study of the ..

Research Grants65

  1. Controlled delivery of polypeptide hormone calcitonin
    Jagdish Singh; Fiscal Year: 2013
    ..the released sCT as well as sCT in the gel will be evaluated using Fourier transform infrared spectroscopy, circular dichroism, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, native sodium dodecyl sulfate-polyacrylamide gel ..
  2. 3D Differentiation of Mesenchymal Stem Cells in Peptide Amphiphile Matrices
    John B Matson; Fiscal Year: 2011
    ..of the gel will be accomplished using electron microscopy, small-angle X-ray scattering, rheology, and circular dichroism. A combination of staining, gene expression, and protein expression will be used to quantify the extent of ..
  3. Alkylammonium Formate Mobile Phase Modifiers for Protein Liquid Chromatography
    NEIL DANIELSON; Fiscal Year: 2009
    ..The synthesis and purification of MAF and EAF will be studied to reduce the UV background down to 220 nm so circular dichroism can be used to study the tertiary and secondary structures of model proteins such as cytochrome c, bovine ..
  4. ABNORMAL BIGH3 AGGREGATIONS IN CORNEAL DYSTROPHIES
    Andrew J W Huang; Fiscal Year: 2011
    ..The proposal intends to investigate the conformations of native and mutant bIGH3 proteins by circular dichroism (CD) spectroscopy, intrinsic fluorescence spectroscopy, fourier transformed infrared resonance (FTIR) ..
  5. BIOGENESIS AND INHIBITION OF BIOFILM-ASSOCIATED BACTERIAL AMYLOID
    Scott J Hultgren; Fiscal Year: 2013
    ..This fiber is made up of the major subunit CsgA, and a minor subunit CsgB. Using Thioflavin T fluorescence, circular dichroism and atomic force microscopy the in vitro mechanism by which CsgB nucleates CsgA fibrillization has been ..
  6. Spectroscopic Studies of Active Sites in Copper Proteins
    Edward I Solomon; Fiscal Year: 2011
    ..the application of a wide variety of spectroscopic (absorption, variable-temperature variable-field magnetic circular dichroism, resonance Raman, EPR, SQUID magnetic susceptibility, X-ray absorption, etc...
  7. Alpha-Synuclein-Metal Complexes and Oxidative Stress in Parkinson's Disease
    Feimeng Zhou; Fiscal Year: 2012
    ..spectroscopy) will be used to investigate the binding and redox reactions, whereas atomic force microscopy, circular dichroism, and dynamic light scattering will be employed to follow the aggregation processes...
  8. DEVELOPING SOLUTES AS STRUCTURAL/MECHANISTIC PROBES OF PROTEIN-DNA INTERACTIONS
    M Thomas Record; Fiscal Year: 2013
    ..interactions of solutes with uncharged biopolymer surfaces exposed upon unfolding/melting, including circular dichroism studies with marginally-stable proteins (alpha-helical alanine-based peptides and the globular DNA-binding-..
  9. Benzophenones: Novel natural agents which inhibits HIV and Candida
    Ramiro Mendonca Murata; Fiscal Year: 2013
    ..inhibit HIV infection through interaction with viral gp120 or mammalian cell receptors for the virus using circular dichroism, biosensor analysis and Aim 3) Evaluate and characterize the anti-fungal effect of benzophenones against C...
  10. Benzophenones: Novel natural agents which inhibits HIV and Candida
    Ramiro Mendonca Murata; Fiscal Year: 2011
    ..inhibit HIV infection through interaction with viral gp120 or mammalian cell receptors for the virus using circular dichroism, biosensor analysis and Aim 3) Evaluate and characterize the anti-fungal effect of benzophenones against C...
  11. Factors influencing regulation of the dynamics of the actin filament pointed end
    Alla S Kostyukova; Fiscal Year: 2013
    ..Binding will be assayed using native gel electrophoresis, cross-linking, and circular dichroism spectroscopy...
  12. Ethanol and Bcl-2 Gene Interactions in the Developing CNS
    Marieta B Heaton; Fiscal Year: 2012
    ..In addition, protein purification and circular dichroism (CD) methodologies will be used to examine protein structure...
  13. PRODUCT DEVELOPMENT OF A MEMBRANE TETRASPANIN VACCINE AGAINST SCHISTOSOMIASIS
    CHARLES RUSSELL MIDDAUGH; Fiscal Year: 2011
    ..Among the innovations in this proposal, the recombinant antigen will undergo biophysical profiling (i.e., circular dichroism, fluorescence, and light scattering), together with GRAS (generally regarded as safe)-designated exicpietns ..
  14. Role of Helix Formation in Mediating Protein-Protein Interactions
    George I Makhatadze; Fiscal Year: 2010
    ..Experimental methods will include site-directed mutagenesis, calorimetry, fluorescence and circular dichroism spectroscopies, structural and relaxation NMR analysis...
  15. Structure-Activity Relationship in Host Defense Peptides
    GREGORY ALEXANDER CAPUTO; Fiscal Year: 2010
    ..b>Circular dichroism and Forster resonance energy transfer studies will elucidate peptide folding at the bilayer surface...
  16. Large-Scale Conformational Changes in Regulation of Transcription Initiation
    M Thomas Record; Fiscal Year: 2011
    ..of protein interfaces to operator binding will be investigated for the crosslinked dimeric headpiece by ITC, circular dichroism and a competitive nitrocellulose filter assay with intact lac repressor tetramer...
  17. INTRINSICALLY DISORDERED PROTEINS IN BIOMINERALIZATION
    JANET M OLDAK; Fiscal Year: 2013
    ..Aim II: To use circular dichroism (CD) and fluorescence titrations to determine the conformational changes and strength of interactions ..
  18. Enhancing In Vitro Antimicrobial Activity of Common Antibiotics with Cyclopeptide
    Maria Ngu-Schwemlein; Fiscal Year: 2012
    ..associated with stabilizing bacterial membrane will be studied by isothermal titration calorimetry (ITC), circular dichroism and X-ray diffraction methods...
  19. The Role of Ribosomal RNA Modifications
    Christine S Chow; Fiscal Year: 2012
    ..switching mechanism in H69 in humans and bacteria through a variety of biophysical studies (NMR, circular dichroism, fluorescence spectroscopy), 2) use chemical probing and biological assays on full-length ribosomal RNAs and ..
  20. Caspase-6 allosteric inhibitors: activity probes and neurodegeneration treatment
    JEANNE ANN HARDY; Fiscal Year: 2013
    ..The allosterically inhibited conformation targeted in this screen exhibits a unique signature in the circular dichroism spectra...
  21. Development of a Biomimetic Vitreous Substitute
    Nathan Ravi; Fiscal Year: 2013
    ..their hydrogels will be characterized using nuclear magnetic resonance, Fourier transform infrared, raman, circular dichroism, and light- scattering spectroscopies, and rheology...
  22. Characterizing a novel hemophore from pathogenic and non-pathogenic mycobacteria.
    LISA M MCMATH; Fiscal Year: 2010
    ..2) To characterize the heme environments in both hemophores. Techniques include circular dichroism, UV/vis and electron paramagnetic resonance spectroscopy, and heme affinity calculations for each hemophore...
  23. Microvesicles and exosomes as a cancer biomarker detected with designed peptides
    NOAH KASTELOWITZ-LIEBERMAN; Fiscal Year: 2013
    ..Completion of Aim 1 will provide a library of highly selective curvature binding peptides. In Aim 2, circular dichroism spectroscopy, isothermal titration calorimetry, and fluorescent enhancement assays will be conducted using ..
  24. Structures and complexes of vertebrate telomere repeat DNAs.
    PHILIP BOLTON; Fiscal Year: 2009
    ..The methods to be validated include circular dichroism and hydroxyl radical cleavage...
  25. Chiroptical Induced CPL-Based Tool as a Probe of Biological Substrates
    Gilles Muller; Fiscal Year: 2013
    ..The central hypothesis of this proposal is that CPL is an advancement over the common circular dichroism (CD) method due to its superiority in sensitivity, reliability, ease of use, and minimal sample preparation...
  26. Conformational Switching in Proteins
    MATTHEW HJ CORDES; Fiscal Year: 2012
    ..site-directed mutagenesis, chimeragenesis, protein design, biophysical characterization methods such as circular dichroism and analytical ultracentrifugation, and high-resolution structure methods including NMR spectroscopy and X-..
  27. Assembly of Biological Iron-Sulfur Clusters
    Michael K Johnson; Fiscal Year: 2012
    ..of biophysical spectroscopic techniques (electron paramagnetic resonance, absorption, magnetic and natural circular dichroism, resonance Raman, and M"ssbauer) that can probe the nature and detailed properties of iron or iron-sulfur ..
  28. Interactions of C-Reactive Protein with Highly Curved Lipid Membranes
    Scott M Reed; Fiscal Year: 2013
    ..Specifically, this aim will utilize fluorescence spectroscopy and circular dichroism to test whether CRP responds to all membranes in a similar manner or whether each curvature results in a ..
  29. Spectroscopic and Computational Investigation of Copper Monooxygenases
    RYAN COWLEY; Fiscal Year: 2013
    ..application of advanced spectroscopies such as resonance Raman, electron paramagnetic resonance, magnetic circular dichroism, and synchrotron-based methods, and in combination with computational methods, particularly density ..
  30. Bringing Modern Circular Dichroism Instrumentation to Notre Dame Researchers
    PATRICIA LOUISE CLARK; Fiscal Year: 2010
    ..b>Circular dichroism (CD) spectroscopy has been an essential component of the biophysical tool kit for decades, exploiting the ..
  31. Studies of the Activation Mechanisms of Visual Pigments
    David S Kliger; Fiscal Year: 2013
    ..Time-resolved measurements with polarized light, including linear dichroism (LD) and circular dichroism (CD) will be used to obtain more detailed structural information about the activation mechanism...
  32. Chemosensory receptors and the basis of specificity
    STEVEN MUNGER; Fiscal Year: 2009
    ..the T1R ligand-binding pockets, in the presence and absence of ligands, will be solved by a combination of circular dichroism spectrophotometry and X-ray crystallography of T1R N-terminal domains...
  33. Allosteric Binding in Antibodies and Protein Antigens
    Robert C Blake; Fiscal Year: 2011
    ..asymmetric field flow fractionation, isothermal titration and differential scanning calorimetry, and circular dichroism and other spectroscopic measurements...
  34. Near-Infrared VCD of Chiral Pharmaceuticals
    Laurence Nafie; Fiscal Year: 2004
    ..DESCRIPTION: The objective of the proposed research is to apply Fourier transform near-infrared vibrational circular dichroism (FT-near-IRVCD), as a new in situ probe of molecular chirality, to the analysis of pharmaceutical molecules ..
  35. Design and Characterization of a Single-chain Hemoglobin
    Spencer J Anthony Cahill; Fiscal Year: 2010
    ..Our lab will characterize the structures of the mutant globins using near-UV circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies...
  36. Purchase of a CD Spectropolarimeter
    Deborah S Wuttke; Fiscal Year: 2010
    ..applicant): A group of researchers at the University of Colorado, Boulder, are requesting a state-of-the-art circular dichroism spectropolarimeter (CD) for biomolecular studies...
  37. Natural Products for Treatment of Emergent AIDS-Related Pathogens
    Tadeusz F Molinski; Fiscal Year: 2013
    ..structure elucidation at sub- micromole scale based on integration of microcryoprobe NMR, mass spectrometry, circular dichroism and organic synthesis, and a new deep-chemical diversity 'bottom-up'(DCD-BU) approach for ..
  38. Silicone-free glass pre-filled syringe system for Biological Drugs.
    VINAY SAKHRANI; Fiscal Year: 2009
    ..TriboGlide(r) glass syringes for effects on secondary and tertiary structural changes of the proteins, using circular dichroism and fluorescence spectroscopy...
  39. Biotechnology Research Partnerships Renewal: Aggregation of Therapeutic Proteins
    Theodore W Randolph; Fiscal Year: 2013
    ..neutron scattering, small angle x-ray scattering micro-flow imaging, surface micro-rheology, chromatography, circular dichroism spectroscopy and infrared spectroscopy...
  40. Membrane Protein Stability
    Karen G Fleming; Fiscal Year: 2012
    ..In a second aim, we will use kinetic and thermodynamic experiments employing SDS-PAGE, circular dichroism, fluorescence spectroscopy and analytical ultracentrifugation to determine the steps involved in folding and ..
  41. CHIROPTICAL SPECTROSCOPY OF METALLOPROTEINS
    Philip Stephens; Fiscal Year: 1990
    ..be employed involve optical spectroscopy using polarized light and (in part) magnetic fields, specifically circular dichroism (CD) and magnetic circular dichroism (MCD)...
  42. The experimental energy landscape and protein function
    Vincent J Hilser; Fiscal Year: 2013
    ..We will perform binding and stability measurements using isothermal titration calorimetry (ITC), circular dichroism (CD) monitored thermal unfolding and hydrogen exchange (HX), and we will monitor the kinetics of the ..
  43. Anti-Cancer RNA Nanoconjugates
    Adam Wanekaya; Fiscal Year: 2013
    ..as studied by electron microscopy, UV/Vis, dynamic laser light scatter, and the more innovative circular dichroism (CD) spectroscopy...
  44. STRUCTURE/FUNCTION STUDIES ON PLASMINOGEN AND PLASMIN
    Francis Castellino; Fiscal Year: 1999
    ..of these domains will be investigated through a powerful combination of scanning and titration calorimetry, circular dichroism, high-field NMR, structural predictive techniques, synthesis of functional peptides and evaluation of the ..
  45. Proteins in the Molecular Mechanisms of Tear Film Formation
    Ben J Glasgow; Fiscal Year: 2013
    ..on the technologic innovations developed in our laboratory of site directed tryptophan fluorescence and circular dichroism to further this effort...
  46. Mechanisms of Adaption: Genotype to Molecular Phenotype to Growth Rate
    Jennifer L Knies; Fiscal Year: 2010
    ..Measure the thermostability of these same alleles with circular dichroism. Use the kinetic data to calculate the activation energy for each allele...
  47. Membrane catalyzed amyloid formation in diabetes studied with 2D IR spectroscopy
    Martin T Zanni; Fiscal Year: 2013
    ..As a result, almost all experimental structural information comes from circular dichroism measurements, which provide only a rudimentary characterization of the peptide structure...
  48. Impact of Alport syndrome mutations and natural interruptions on collagen folding
    EILEEN HWANG; Fiscal Year: 2012
    ..Recombinant collagen will be used to study the effect of interruptions on folding kinetics by circular dichroism, trypsin susceptibility, fluorescence, and electron microscopy...
  49. Understanding the Effect of N-Terminal Acetylation on alpha-Synuclein Toxicity
    JENNIFER BEIERLEIN; Fiscal Year: 2013
    ..Structural studies utilizing circular dichroism and NMR spectroscopy will examine any structural changes resulting from the posttranslational modifications...
  50. Refining &Implementing Supramolecular Methods for HTS of EE &Concentration
    Eric V Anslyn; Fiscal Year: 2013
    ..synthetic receptors that are targeted to classes of chiral functional groups, and record absorbance or circular dichroism spectra for diastereomeric or enantiomeric complex formation. LDA, PCA, or ANN interprets the optical data...
  51. Gamma crystallin modifications &mechanisms of lens opacity
    Jayanti Pande; Fiscal Year: 2013
    ..v.-visible, circular dichroism, fluorescence, FTIR, Raman and NMR), and stabilities of the modified forms of human [unreadable]D-, [..
  52. Signaling Biophysics of Protein-GTPase Interactions
    Matthias Buck; Fiscal Year: 2009
    ..Global thermodynamic perturbations will be followed by fluorescence and circular dichroism spectroscopy, site specific changes by amide hydrogen exchange in conjunction with NMR spectroscopy...
  53. RNA TERTIARY STRUCTURE AND PROTEIN INTERACTIONS
    W David Wilson; Fiscal Year: 1999
    ..involves three major experimental approaches; (i) chemical and enzymatic probing; (i) thermal melting and circular dichroism (CD); (iii) NMR and molecular modeling...
  54. Circular Dichroism Spectropolarimeter with fluorescence acquisition and temperatu
    Robert M Straubinger; Fiscal Year: 2010
    ..by applicant): In the NIH-supported core projects conducted by the eight co-investigators of this proposal, Circular Dichroism (CD) spectropolarimetry provides a unique window into dynamic aspects of protein conformation and unfolding, ..
  55. Computational and Experimental Studies of the Amyloid Fibril Formation by PAPf39
    George I Makhatadze; Fiscal Year: 2013
    ..The computational study will be validated by a battery of experimental methods including NMR, atomic force microscopy, fluorescence and circular dichroism spectroscopies, and hydrogen exchange mass spectroscopy.
  56. TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
    David Kliger; Fiscal Year: 2002
    This project will apply techniques for fast time-resolved magnetic circular dichroism (TRMCD) and magnetic optical rotatory dispersion (TRMORD) spectroscopies to the study of function and folding in heme proteins...
  57. Fast Kinetic Studies of Protein Folding and Function
    David Kliger; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): This project applies techniques for fast time-resolved magnetic circular dichroism (TRMCD), natural circular dichroism (TRCD, and ordinary absorption spectroscopies to the study of function in heme ..
  58. PROTEIN BINDING DOMAINS--EUKARYOTIC FIVE-S RRNA & RDNA
    Paul Huber; Fiscal Year: 1991
    ..We will test this proposal in two ways. Using circular dichroism spectroscopy we will determine the conformation of the DNA when TFIIIA binds to the 5S gene...
  59. PHYSICAL STUDIES OF CONFORMATIONAL CHANGE IN CHROMATIN
    RODNEY HARRINGTON; Fiscal Year: 1990
    ..I. Principal physical methods will include flow birefringence, circular dichroism and high resolution theraml denaturation...
  60. Biogenesis and Function of Bacterial Amyloid fibers
    Matthew Chapman; Fiscal Year: 2005
    ..The structural and tinctoral properties of purified CsgB will be determined by circular dichroism (CD) spectroscopy and by Congo red (CR) and thioflavin T (thT) binding assays...
  61. Ultraviolet Raman Spectroscopic Studies of Protein Dynamics
    Thomas G Spiro; Fiscal Year: 2013
    ..and time-resolved UVRR experiments will be supported by simultaneous fluorescence monitoring, as well as computational molecular dynamics simulations and static nuclear magnetic resonance, circular dichroism, and UV-Vis spectroscopy.
  62. MALIC ENZYME FROM ASCARIS SUUM
    Ben Harris; Fiscal Year: 2002
    ..The elucidation of the structure of malic enzyme will be done by X-ray crystallography, circular dichroism, and fluorescence. Crystals of the ascarid enzyme have been obtained which diffract to 2...
  63. STUDIES ON RHODOPSIN & AGE-RELATED MACULAR DEGENERATION
    Koji Nakanishi; Fiscal Year: 2006
    ..changes of Rh upon activation, the interactions between meta-II and Gt and the determining factors of their circular dichroism (CD) and ultra-violet (UV) spectra remain to be clarified...
  64. ANTIMICROBIAL PEPTIDES OF LEUKOCYTES
    Robert Lehrer; Fiscal Year: 1999
    ..g., circular dichroism, disulfide pairing, etc.)...
  65. STRUCTURAL/FUNCTIONAL PROPERTIES OF HUMAN LIPOPROTEIN A
    Joel Morrisett; Fiscal Year: 1992
    ..each apoprotein on the secondary structure, tertiary structure, and cell receptor binding of the other using circular dichroism, intrinsic fluorescence, and cell culture techniques...