ultraviolet spectrophotometry

Summary

Summary: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Top Publications

  1. ncbi A validated LC method for the determination of clopidogrel in pharmaceutical preparations
    A Mitakos
    School of Pharmacy, Division of Pharmaceutical Chemistry, University of Athens, Panepistimiopolis, Zografou, 157 71, Athens, Greece
    J Pharm Biomed Anal 28:431-8. 2002
  2. ncbi Simultaneous analysis of dehydroacetic acid, benzoic acid, sorbic acid and salicylic acid in cosmetic products by solid-phase extraction and high-performance liquid chromatography
    E Mikami
    Aichi Prefectural Institute of Public Health, 7 6 Nagare, Tsuji machi, Kita ku, Nagoya 462 8576, Japan
    J Pharm Biomed Anal 28:261-7. 2002
  3. ncbi Assay method for quality control and stability studies of a new CVS disorder agent (compound 93/478)
    A K Dwivedi
    Division of Pharmaceutics, Central Drug Research Institute, Chhattar Manzil Palace M G Marg, Lucknow 226001, India
    J Pharm Biomed Anal 33:33-8. 2003
  4. ncbi Rapid determination of aristolochic acid I and II in Aristolochia plants from different regions by beta-cyclodextrin-modified capillary zone electrophoresis
    Wei Li
    The Key Lab of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China
    J Chromatogr A 1049:211-7. 2004
  5. ncbi Enantioselective analysis of the metabolites of hydroxychloroquine and application to an in vitro metabolic study
    Carmem Dickow Cardoso
    Departamento de Quimica, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto USP, Av Bandeirantes 3900, 14040 901 Ribeirao Preto, Sao Paulo, Brazil
    J Pharm Biomed Anal 37:703-8. 2005
  6. ncbi Following G-quartet formation by UV-spectroscopy
    J L Mergny
    Laboratoire de Biophysique, Muséum National d Histoire Naturelle INSERM U201, CNRS URA 481, Paris, France
    FEBS Lett 435:74-8. 1998
  7. ncbi Effect of secondary structure on the thermodynamics and kinetics of PNA hybridization to DNA hairpins
    S A Kushon
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213-3890, USA
    J Am Chem Soc 123:10805-13. 2001
  8. ncbi Analysis of thermal melting curves
    Jean Louis Mergny
    Laboratoire de Biophysique, INSERM UR565, CNRS UMR 5153, Museum national d histoire naturelle, 75231 Paris, France
    Oligonucleotides 13:515-37. 2003
  9. pmc Structure of the human telomere in K+ solution: a stable basket-type G-quadruplex with only two G-tetrad layers
    Kah Wai Lim
    School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore
    J Am Chem Soc 131:4301-9. 2009
  10. pmc Optical absorption assay for strand-exchange reactions in unlabeled nucleic acids
    Besik I Kankia
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Nucleic Acids Res 32:e154. 2004

Detail Information

Publications214 found, 100 shown here

  1. ncbi A validated LC method for the determination of clopidogrel in pharmaceutical preparations
    A Mitakos
    School of Pharmacy, Division of Pharmaceutical Chemistry, University of Athens, Panepistimiopolis, Zografou, 157 71, Athens, Greece
    J Pharm Biomed Anal 28:431-8. 2002
    ..12 and 0.39 micro ml(-1), respectively. The method was applied in the quality control of commercial tablets and content uniformity test and proved to be suitable for rapid and reliable quality control...
  2. ncbi Simultaneous analysis of dehydroacetic acid, benzoic acid, sorbic acid and salicylic acid in cosmetic products by solid-phase extraction and high-performance liquid chromatography
    E Mikami
    Aichi Prefectural Institute of Public Health, 7 6 Nagare, Tsuji machi, Kita ku, Nagoya 462 8576, Japan
    J Pharm Biomed Anal 28:261-7. 2002
    ..5 ng for DHA, 4.0 ng for BA, 2.0 ng for SOA and 5.5 ng for SA. The procedure described here is simple, selective and is suitable for quality control of finished cosmetic products...
  3. ncbi Assay method for quality control and stability studies of a new CVS disorder agent (compound 93/478)
    A K Dwivedi
    Division of Pharmaceutics, Central Drug Research Institute, Chhattar Manzil Palace M G Marg, Lucknow 226001, India
    J Pharm Biomed Anal 33:33-8. 2003
    ..5 microg/ml. The calibration curves were linear in the range 1.5-62 microg/ml. Reproducibility of the method was determined by inter and intra assay variation, which were <10%...
  4. ncbi Rapid determination of aristolochic acid I and II in Aristolochia plants from different regions by beta-cyclodextrin-modified capillary zone electrophoresis
    Wei Li
    The Key Lab of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China
    J Chromatogr A 1049:211-7. 2004
    ..The result indicated high variability in the contents of aristolochic acids due to different species and regions. The comparison of CZE method with high performance liquid chromatography (HPLC) was also discussed...
  5. ncbi Enantioselective analysis of the metabolites of hydroxychloroquine and application to an in vitro metabolic study
    Carmem Dickow Cardoso
    Departamento de Quimica, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto USP, Av Bandeirantes 3900, 14040 901 Ribeirao Preto, Sao Paulo, Brazil
    J Pharm Biomed Anal 37:703-8. 2005
    ..The metabolic study demonstrated that metabolism is stereoselective for HCQ. The major metabolites formed in the incubation of racemic HCQ were (-)-(R)-DCQ and (-)-(R)-DHCQ with R/S ratios of 2.2 and 3.3, respectively...
  6. ncbi Following G-quartet formation by UV-spectroscopy
    J L Mergny
    Laboratoire de Biophysique, Muséum National d Histoire Naturelle INSERM U201, CNRS URA 481, Paris, France
    FEBS Lett 435:74-8. 1998
    ..This inverted denaturation profile was observed for all G-quartet-forming oligonucleotides studied so far. This technique is very useful in all cases where intramolecular or intermolecular quadruplex formation is suspected...
  7. ncbi Effect of secondary structure on the thermodynamics and kinetics of PNA hybridization to DNA hairpins
    S A Kushon
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213-3890, USA
    J Am Chem Soc 123:10805-13. 2001
    ..The thermodynamic and kinetic obstacles to hybridization imposed by both target and probe secondary structure are significant concerns for the continued development of antisense agents and especially diagnostic probes...
  8. ncbi Analysis of thermal melting curves
    Jean Louis Mergny
    Laboratoire de Biophysique, INSERM UR565, CNRS UMR 5153, Museum national d histoire naturelle, 75231 Paris, France
    Oligonucleotides 13:515-37. 2003
    ..We present the classic problems encountered during these experiments and try to demonstrate that a number of useful pieces of information can be extracted from these experimental curves...
  9. pmc Structure of the human telomere in K+ solution: a stable basket-type G-quadruplex with only two G-tetrad layers
    Kah Wai Lim
    School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore
    J Am Chem Soc 131:4301-9. 2009
    ..This novel structure highlights the conformational heterogeneity of human telomeric DNA. It establishes a new folding principle for G-quadruplexes and suggests new loop sequences and structures for targeting in human telomeric DNA...
  10. pmc Optical absorption assay for strand-exchange reactions in unlabeled nucleic acids
    Besik I Kankia
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Nucleic Acids Res 32:e154. 2004
    ..Adding the replacement sequence initiates the release of the 'signal' oligonucleotide into solution, which is accompanied by ultraviolet absorption in long-wavelength range...
  11. ncbi Sequence effects in single-base loops for quadruplexes
    Aurore Guédin
    INSERM, U565, Acides nucléiques dynamique, ciblage et fonctions biologiques, 43 rue Cuvier, CP26, F 75231 Paris Cedex 05, France
    Biochimie 90:686-96. 2008
    ....
  12. ncbi Determination of amlodipine in pharmaceutical dosage forms by liquid chromatography and ultraviolet spectrophotometry
    Marcelo Donadel Malesuik
    Universidade Federal de Santa Maria, Departamento de Farmácia Industrial, Santa Maria, CEP 97 119 900, RS, Brazil
    J AOAC Int 89:359-64. 2006
    ..The proposed methods are precise and accurate and can be applied directly and easily to the oral pharmaceutical preparations of amlodipine...
  13. ncbi Ultraviolet spectrophotometry as an index parameter for estimating the biochemical oxygen demand of domestic wastewater
    M Nataraja
    The Cadmus Group, Inc, 1600 Wilson Blvd, Suite 500, Arlington, VA 22209, USA
    Environ Technol 27:789-800. 2006
    ..However, such relationships are likely to be wastewater and treatment plant specific and variable with time and treatment...
  14. pmc The guanine-rich fragile X chromosome repeats are reluctant to form tetraplexes
    Petr Fojtik
    Academy of Sciences of the Czech Republic, Institute of Biophysics, Kralovopolska 135, CZ 612 65 Brno, Czech Republic
    Nucleic Acids Res 32:298-306. 2004
    ..Rather, a hairpin of the fragile X repeats, whose stability increases with the repeat length, is the probable structure responsible for the repeat expansion in genomes...
  15. ncbi The photoadaptive response to ultraviolet exposure in human skin using ultraviolet spectrophotometry
    Alison Hennessy
    Department of Dermatology, University of Edinburgh, Edinburgh EH3 9HA, UK
    Photodermatol Photoimmunol Photomed 21:229-33. 2005
    ..The purpose of this study was to estimate independently these changes occurring in the epidermis following repeated exposure to UV in two groups with differing degrees of constitutive pigmentation...
  16. ncbi Structural polymorphism exhibited by a homopurine.homopyrimidine sequence found at the right end of human c-jun protooncogene
    Sarika Saxena
    Nucleic Acids Research Laboratory, Department of Chemistry, University of Delhi North Campus, Delhi 110007, India
    Arch Biochem Biophys 471:95-108. 2008
    ..This self-association property of oligonucleotides might limit their use as duplex targets in triplex formation. Study is also relevant for understanding structural and biological properties of DNA sequence containing homopurine tracts...
  17. pmc A novel chair-type G-quadruplex formed by a Bombyx mori telomeric sequence
    Samir Amrane
    Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371
    Nucleic Acids Res 37:931-8. 2009
    ..Our result highlights the effect of G-tract length on the folding topology of G-quadruplexes, but also poses the question of whether a similar chair-type G-quadruplex fold exists in the human telomeric sequences...
  18. ncbi Oxidase-peroxidase reaction: kinetics of peroxidase-catalysed oxidation of 2-aminophenol
    Mihaela Puiu
    Department of Physical Chemistry, University of Bucharest, Bucharest, Romania
    Bioprocess Biosyst Eng 31:579-86. 2008
    ..Sensitivity analysis allowed to conclude that both oxidase and peroxidase cycles occur in 2-aminophenol oxidation...
  19. ncbi Fungal metabolite screening: database of 474 mycotoxins and fungal metabolites for dereplication by standardised liquid chromatography-UV-mass spectrometry methodology
    Kristian Fog Nielsen
    Mycology Group, Technical University of Denmark, Building 221, DK 2800 Kgs Lyngby, Denmark
    J Chromatogr A 1002:111-36. 2003
    ..This was done by UV spectroscopy combined with accurate mass determination of adduct and fragment ions obtained by high-resolution orthogonal time-of-flight MS...
  20. pmc The effect of chemical modifications on the thermal stability of different G-quadruplex-forming oligonucleotides
    Barbara Saccà
    Laboratoire de Biophysique, Muséum National d Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France
    Nucleic Acids Res 33:1182-92. 2005
    ....
  21. pmc Revised UV extinction coefficients for nucleoside-5'-monophosphates and unpaired DNA and RNA
    Michael J Cavaluzzi
    Department of Chemistry and Graduate Program in Structural Biology, Biochemistry and Biophysics, Syracuse University, NY 13244 4100, USA
    Nucleic Acids Res 32:e13. 2004
    ..Methods are described here to accurately measure concentrations of nucleotides by nuclear magnetic resonance. NMR can be used to accurately determine concentrations (and extinction coefficients) of biomolecules within 1%...
  22. ncbi Multi-center evaluation of analytical performance of the microparticle enzyme immunoassay for sirolimus
    D Wilson
    Abbott Laboratories, Abbott Park, IL 60064, USA
    Clin Biochem 39:378-86. 2006
    ..This study evaluated the analytical characteristics of the new Abbott microparticle enzyme immunoassay (MEIA) for sirolimus...
  23. ncbi Characterization of the Desulfovibrio desulfuricans ATCC 27774 DsrMKJOP complex--a membrane-bound redox complex involved in the sulfate respiratory pathway
    Ricardo H Pires
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, 2781 901 Oeiras, Portugal
    Biochemistry 45:249-62. 2006
    ..Further five different [4Fe-4S](2+/1+) centers are observed during a redox titration followed by EPR. The role of the DsrMKJOP complex in the sulfate respiratory chain of Desulfovibrio spp. is discussed...
  24. ncbi An evaluation of the Abbott IMx sirolimus assay in relation to a high-performance liquid chromatography-ultraviolet method
    Roger N Johnson
    Department of Chemical Pathology, LabPlus, Auckland City Hospital, Auckland, New Zealand
    Ann Clin Biochem 42:394-7. 2005
    ..An immunoassay that uses readily available equipment offers a more convenient alternative...
  25. ncbi Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea
    Gerd J Mander
    Max Planck Institut fur terrestrische Mikrobiologie, Marburg, Germany
    Eur J Biochem 269:1895-904. 2002
    ..Hence, Hdr and the A. fulgidus enzyme not only share sequence similarity, but may also have a similar active site and a similar catalytic function...
  26. ncbi Resolution of the aerobic respiratory system of the thermoacidophilic archaeon, Sulfolobus sp. strain 7. III. The archaeal novel respiratory complex II (succinate:caldariellaquinone oxidoreductase complex) inherently lacks heme group
    T Iwasaki
    Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan
    J Biol Chem 270:30902-8. 1995
    ..This further indicates that the heme group of several respiratory complexes II may not be involved in the redox intermediates of the electron transfer from succinate to quinone...
  27. ncbi Structural competition involving G-quadruplex DNA and its complement
    Wei Li
    High Technology Research Center, Konan University, 8 9 1 Okamoto, Higashinada ku, Kobe 658 8501, Japan
    Biochemistry 42:11736-44. 2003
    ..5 reflects the fact that pH significantly influences the structural competition between the G-quadruplex, the I-motif, and the Watson-Crick duplex, which also implies a possible biological role for I-motifs in biological regulation...
  28. pmc Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats
    Samir Amrane
    Laboratoire de Biophysique, Museum national d histoire naturelle, USM 503 INSERM UR 565, CNRS UMR 8646, 43 rue Cuvier, 75231 Paris Cedex 05, France
    Nucleic Acids Res 33:4065-77. 2005
    ..Similar results were also found for CAG oligomers, suggesting that this observation may be extended to various trinucleotide repeats-containing sequences...
  29. ncbi Loop-length-dependent folding of G-quadruplexes
    Pascale Hazel
    Cancer Research UK Biomolecular Structure Group, The School of Pharmacy, University of London, 29 39 Brunswick Square, London WC1N 1AX, UK
    J Am Chem Soc 126:16405-15. 2004
    ..Multiple conformations of each structure are likely to coexist in solution, as they were calculated to have very similar free energies...
  30. ncbi Structural organization of essential iron-sulfur clusters in the evolutionarily highly conserved ATP-binding cassette protein ABCE1
    Dominik Barthelme
    Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe University, Max von Laue Strasse 9, D 60439 Frankfurt am Main, Germany
    J Biol Chem 282:14598-607. 2007
    ..Notably, a lethal mutation of the cysteine at position 4 can be rescued by ligand swapping with an adjacent, extra cysteine conserved among all eukaryotes...
  31. ncbi Calorimetric unfolding of the bimolecular and i-motif complexes of the human telomere complementary strand, d(C(3)TA(2))(4)
    Mahima Kaushik
    Department of Pharmaceutical Sciences, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE 68198 6025, USA
    Biophys Chem 126:154-64. 2007
    ..Furthermore, an enthalpy of 3.2 kcal/mol (bimolecular complex) and 5.0 kcal/mol (i-motif) is estimated for a single C*C(+)/C*C(+) base-pair stack...
  32. ncbi Metabolic profiling of flavonoids in Lotus japonicus using liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry
    Hideyuki Suzuki
    Kazusa DNA Research Institute, Kazusa Kamatari 2 6 7, Kisarazu 292 0818, Japan
    Phytochemistry 69:99-111. 2008
    ..Developmental changes in the flavonoid profiles demonstrated that kaempferol glycosides increased promptly after germination. In contrast, quercetin glycosides, predominant flavonoids in the seeds, were not detectable in growing leaves...
  33. pmc Thermal difference spectra: a specific signature for nucleic acid structures
    Jean Louis Mergny
    Laboratoire de Biophysique, Museum national d histoire naturelle, USM503, INSERM U 565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France
    Nucleic Acids Res 33:e138. 2005
    ..TDS complements circular dichroism as a tool for the structural characterization of nucleic acids in solution...
  34. ncbi Free radical chemistry of flavan-3-ols: determination of thermodynamic parameters and of kinetic reactivity from short (ns) to long (ms) time scale
    Cécile Cren-Olivé
    Laboratoire de Chimie Organique et Macromoléculaire, Equipe Polyphenols, Universite des Sciences et Technologies de Lille, UMR CNRS 8009, Bâtiment C4, 59655 Villeneuve d Ascq cedex, Lille, France
    J Am Chem Soc 124:14027-38. 2002
    ..58) were performed. These values are discussed and compared to the prediction of the density functional theory calculations made on the different species catechin, catechin phenoxyl, and catechin phenolate for each phenolic site...
  35. ncbi Comparison between solution-phase stability and gas-phase kinetic stability of oligodeoxynucleotide duplexes
    V Gabelica
    Laboratoire de Spectrometrie de masse, Bat B6c, Universite de Liege, B 4000 Liege, Belgium
    J Mass Spectrom 36:397-402. 2001
    ..We also draw attention to the fact that by source CID, it is the kinetic stability of the complexes that is probed. In particular, this implies that only complexes of the same size can be compared...
  36. ncbi Triplex and quadruplex DNA structures studied by electrospray mass spectrometry
    Frederic Rosu
    Laboratoire de Biospectroscopie, Institut de Chimie Bat B6c, Universite de Liege, B 4000 Liege, Belgium
    Rapid Commun Mass Spectrom 16:1729-36. 2002
    ..This is the same dissociation pathway as in solution. To our knowledge this is the first report of a triplex DNA structure by electrospray mass spectrometry...
  37. pmc Stabilities of HIV-1 DIS type RNA loop-loop interactions in vitro and in vivo
    Christina Lorenz
    Max F Perutz Laboratories, Department of Biochemistry, University of Vienna, Dr Bohrgasse 9 5, A 1030 Vienna, Austria
    Nucleic Acids Res 34:334-42. 2006
    ..The most stable interactions are the naturally occurring HIV-1 DIS MAL and LAI derived loops with the motif (5' A(A)/(G)N6A 3'), emphasizing the crucial role of stable kissing complexes in HIV genome dimerization...
  38. ncbi Simultaneous determination of valsartan and hydrochlorothiazide in tablets by first-derivative ultraviolet spectrophotometry and LC
    E Satana
    Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, 06330, Ankara, Turkey
    J Pharm Biomed Anal 25:1009-13. 2001
    First-derivative ultraviolet spectrophotometry and high-performance liquid chromatography (HPLC) were used to determine valsartan and hydrochlorothiazide simultaneously in combined pharmaceutical dosage forms...
  39. ncbi Expression, purification and characterization of cytochrome P450 Biol: a novel P450 involved in biotin synthesis in Bacillus subtilis
    A J Green
    Department of Pure and Applied Chemistry, University of Strathclyde, The Royal College, Glasgow, UK
    J Biol Inorg Chem 6:523-33. 2001
    ..Substrate hydroxylation at the omega-terminal position was demonstrated by turnover of the chromophoric fatty acid para-nitrophenoxydodecanoic acid, and by separation of product from the reaction of P450 BioI with myristic acid...
  40. ncbi Binding of antitumor antibiotic daunomycin to histones in chromatin and in solution
    Azra Rabbani
    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
    Biochemistry 43:16497-504. 2004
    ..The studies described here reveal the occurrence of a previously undisclosed mechanism for the antitumor activity of anthracycline drugs at the chromatin level...
  41. ncbi Association of the minor groove binding drug Hoechst 33258 with d(CGCGAATTCGCG)2: volumetric, calorimetric, and spectroscopic characterizations
    Feixue Han
    Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, 19 Russell Street, Toronto, Ontario M5S 2S2, Canada
    Biochemistry 44:9785-94. 2005
    ..The latter observation is consistent with the "lock-and-key" mode of minor groove binding...
  42. ncbi HIV-1 nucleocapsid protein activates transient melting of least stable parts of the secondary structure of TAR and its complementary sequence
    Serena Bernacchi
    Laboratoire de Pharmacologie et Physico Chimie des Interactions Cellulaires et Moléculaires, UMR 7034 CNRS, Faculte de Pharmacie, Universite Louis Pasteur, 74, route du Rhin, Strasbourg 1, 67401, France
    J Mol Biol 317:385-99. 2002
    ..Also, this activity of NCp7 was found to be dependent on the zinc-finger motifs, since no melting was observed with a fingerless NCp7 peptide...
  43. pmc UVA-visible photo-excitation of guanine radical cations produces sugar radicals in DNA and model structures
    Amitava Adhikary
    Department of Chemistry, Oakland University, Rochester, MI 38309, USA
    Nucleic Acids Res 33:5553-64. 2005
    ....
  44. pmc Mg2+-induced triplex formation of an equimolar mixture of poly(rA) and poly(rU)
    Besik I Kankia
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 1479 Gortner Avenue, St Paul, MN 55108, USA
    Nucleic Acids Res 31:5101-7. 2003
    ..The phase boundary corresponding to the transition between double and triple helical conformations lies near physiological salt concentrations and temperature...
  45. ncbi Formation of a PNA2-DNA2 hybrid quadruplex
    Bhaskar Datta
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 3890, USA
    J Am Chem Soc 125:4111-8. 2003
    ..The formation of this novel supramolecular structure demonstrates a new high-affinity DNA recognition mechanism and expands the scope of molecular recognition by PNA...
  46. ncbi Rapid high-performance liquid chromatographic determination of docetaxel (Taxotere) in plasma using liquid-liquid extraction
    J Ciccolini
    Laboratoire de Toxicocinétique et de Pharmacocinétique, Faculte de Pharmacie, Marseille, France
    J Chromatogr B Biomed Sci Appl 759:299-306. 2001
    ....
  47. ncbi UV-visible spectral identification of the solution-phase and solid-phase permanganate oxidation reactions of thymine acetic acid
    Chinh T Bui
    Genomic Disorders Research Centre and the University of Melbourne, 7th Floor, Daly Wing, St Vincent Hospital, 35 Victoria Parade, Fitzroy, Melbourne, Victoria 3065, Australia
    Bioorg Med Chem Lett 14:1313-5. 2004
    ..This result enables unambiguous interpretation of the absorbance change at 420 nm, as the intermediate permanganate ions could be isolated on the solid supports...
  48. ncbi Reliable HPLC method for therapeutic drug monitoring of frequently prescribed tricyclic and nontricyclic antidepressants
    Wilson Roberto Malfará
    Department of Psychiatry and Psychology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Avenida do Cafe s n, Monte Alegre, CEP 14040 903 Ribeirao Preto, SP, Brazil
    J Pharm Biomed Anal 44:955-62. 2007
    ..The method has been successfully applied to the analysis of real samples, for the drug monitoring of ten frequently prescribed tricyclic and non-tricyclic antidepressant drugs...
  49. ncbi Development and validation of reversed phase high performance liquid chromatography method for determination of dexpanthenol in pharmaceutical formulations
    A U Kulikov
    Laboratory of Pharmacopoeial Analysis, Scientific and Expert Pharmacopoeial Centre, Astronomicheskaya Street 33, 61085 Kharkov, Ukraine
    J Pharm Biomed Anal 43:983-8. 2007
    ..The method can be used for dexpanthenol assay of panthenol aerosol and gel with dexpanthenol as the method separates dexpanthenol from aerosol or gel excipients...
  50. ncbi Diastereomeric and enantiomeric high-performance liquid chromatographic separation of synthetic anisodamine
    Li Min Yang
    Department of Pharmacy, Shanghai Jiao Tong University School of Medicine, South ChongQing Road 280, Shanghai 200025, China
    J Pharm Biomed Anal 43:905-9. 2007
    ..The methods were optimized by studying mobile phase modifiers, concentration of modifier and column temperature. The HPLC method for the simultaneous separation of two pairs of enantiomers of 654-2 has been validated...
  51. ncbi The use of mixed-mode ion-exchange solid phase extraction to characterize pharmaceutical drug degradation
    Margaret S Landis
    Pfizer Global Research and Development, Pharmaceutical Sciences Division, Eastern Point Road MC 4127, Groton, CT 06340, USA
    J Pharm Biomed Anal 44:1029-39. 2007
    ..Several examples of the use of mixed-mode ion-exchange solid phase extraction to illustrate the utility of this technique are presented...
  52. ncbi Characterization of a potential medium for 'biorelevant' in vitro release testing of a naltrexone implant, employing a validated stability-indicating HPLC method
    Sunil S Iyer
    Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University, Richmond, VA 23298 0533, USA
    J Pharm Biomed Anal 43:845-53. 2007
    ..This was applied to an investigation of in vitro drug release. The method has been proven to be suitable for investigation of naltrexone released from the implant...
  53. ncbi HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment
    L Havlíková
    Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic
    J Pharm Biomed Anal 43:1169-73. 2007
    ..6 ml min(-1) was used. UV detection was performed at 239 nm, the total analysis time was about 10 min. The method is suitable for practical routine analysis of topical ointment in the quality control laboratory...
  54. ncbi HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum
    Xiao Ming Wang
    Shanghai Research Center for TCM Modernization, Shanghai Institute of Materia Medica, Shanghai Institute for Biological Sciences, Guo Shoujing Road 199, Zhangjiang, Shanghai, PR China
    J Pharm Biomed Anal 43:1185-90. 2007
    ..Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time...
  55. ncbi A simple sample preparation with HPLC-UV method for estimation of tiropramide from plasma: application to bioequivalence study
    Khan Imran
    Department of Pharmacology, The Bombay College of Pharmacy, Kalina, Santacruz E, Mumbai 400 098, India
    J Pharm Biomed Anal 43:1135-40. 2007
    ..These results indicated that the analytical method was linear, precise and accurate. Test and reference formulation were found to be bioequivalent...
  56. ncbi Quantification of eugenol, luteolin, ursolic acid, and oleanolic acid in black (Krishna Tulasi) and green (Sri Tulasi) varieties of Ocimum sanctum Linn. using high-performance thin-layer chromatography
    Sheetal Anandjiwala
    B V Patel Pharmaceutical Education and Research Development Centre, Pharmacognosy and Phytochemistry Department, Thaltej, Ahmedabad 380 054, Gujarat, India
    J AOAC Int 89:1467-74. 2006
    ..The HPTLC-densitometry methods for the quantification of the 4 markers in O. sanctum leaf will have the applicability in quality control...
  57. ncbi Direct automatic screening of soils for polycyclic aromatic hydrocarbons based on microwave-assisted extraction/fluorescence detection and on-line liquid chromatographic confirmation
    A Criado
    Department of Analytical Chemistry, University of Cordoba, Campus de Rabanales, 14071 Cordoba, Spain
    J Chromatogr A 1050:111-8. 2004
    ..Application to the screening of solid environmental samples with subsequent confirmation of the results is also presented...
  58. ncbi Identification and quantification method of spiramycin and tylosin in feedingstuffs with HPLC-UV/DAD at 1 ppm level
    C Civitareale
    Istituto Superiore di Sanita, Laboratorio Medicina Veterinaria, Viale Regina Elena, 299 I 00161 Rome, Italy
    J Pharm Biomed Anal 36:317-25. 2004
    ..As a verification, the overlapping of UV spectra in the range 220-350 nm between analytical standards and the compounds in the matrix were considered...
  59. ncbi Rapid and simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by high-performance liquid chromatography
    Hossein Amini
    Department of Pharmacology, Neuroscience Research Center, Shaheed Beheshti Medical University, Tehran, Iran
    J Pharm Biomed Anal 43:1146-50. 2007
    ..125-2 microg/ml for TMP and 0.39-50 microg/ml for SMX. The limit of quantification (LOQ) in plasma was 0.125 and 0.39 microg/ml for TMP and SMX, respectively. The method was used for a bioequivalence study...
  60. ncbi Spectrophotometric determination of oxiconazole in topical lotion using methyl orange
    Julie Milano
    Universidade Federal de Santa Maria, Departamento de Farmácia Industrial, Santa Maria, CEP 97119 900 RS, Brazil
    J Pharm Biomed Anal 37:639-42. 2005
    ..9995), precise (intra-day: CV=1.57% and inter-day: CV=1.50%) and accurate (mean recoveries: 99.69%). The results compared favourably with those of the HPLC method...
  61. ncbi Determination of ceftiofur in bovine plasma by HPLC-DAD
    G A Jacobson
    School of Pharmacy, University of Tasmania, GPO BOX 252 26, Hobart, Tasmania 7001, Australia
    J Pharm Biomed Anal 40:1249-52. 2006
    ..15 microg/ml. The assay was successfully applied to bovine plasma following intramuscular injection of ceftiofur sodium. This simplified method is suitable for pharmacokinetic applications involving ceftiofur at normal therapeutic levels...
  62. ncbi Stability studies of ionised and non-ionised 3,4-diaminopyridine: hypothesis of degradation pathways and chemical structure of degradation products
    J A Raust
    Analytical Development Department, Pharmaceutical Establishment of the Paris Hospitals, Agence Générale des Equipements et Produits de Santé, 7 rue du Fer à Moulin, 75005 Paris, France
    J Pharm Biomed Anal 43:83-8. 2007
    ..Owing to the better stability of the salt species, a new pharmaceutical form containing it was developed to assess its stability under ICH standard conditions allowing an industrial manufacture of this drug...
  63. ncbi Development and validation of a simple HPLC method for simultaneous in vitro determination of amoxicillin and metronidazole at single wavelength
    Naser Tavakoli
    Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
    J Pharm Biomed Anal 43:325-9. 2007
    ..13 microg/ml for metronidazole. The determination of the two active ingredients was not interfered by the excipients of the products. Samples were stable in the release media (37 degrees C) and the HPLC injector at least for 12 h...
  64. ncbi LC determination of glimepiride and its related impurities
    Mubeen Ahmad Khan
    Analytical Research Lab, Glenmark Research Centre, Plot A 607, TTC Industrial Area, MIDC, Mahape, 400709 Navi Mumbai, India
    J Pharm Biomed Anal 39:928-43. 2005
    ..The method has been validated as per international guidelines on method validation and can be used for the routine quality control analysis of glimepiride as active pharmaceutical ingredient (API)...
  65. ncbi A new hydrophilic interaction liquid chromatographic (HILIC) procedure for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold formulations
    Mohammed Shahid Ali
    Jamjoom Pharmaceuticals Company Limited, Industrial Area, Phase 5, Jeddah 21442, Saudi Arabia
    J Pharm Biomed Anal 43:158-67. 2007
    ..Mobile phase pH is manipulated to regulate ionization and ion-exchange interaction and thereby retention of compounds...
  66. ncbi Development and validation of a HPLC-UV method for the determination in didanosine tablets
    Antonia Maria Cavalcanti de Oliveira
    Instituto Vital Brazil, Niteroi, RJ 24310 110, Brazil
    J Pharm Biomed Anal 38:751-6. 2005
    ..Hypoxantine was confirmed as the main degradation product. The assay was linear over the concentration range of 50-150 microg/ml (R approximately 0.999). The method was validated for accuracy and precision...
  67. ncbi Method development and validation for the simultaneous determination of cetirizine dihydrochloride, paracetamol, and phenylpropanolamine hydrochloride in tablets by capillary zone electrophoresis
    S Azhagvuel
    Analytical Chemistry Division, Indian Institute of Chemical Technology, Hyderabad 500007, India
    J Pharm Biomed Anal 43:873-8. 2007
    ..There were no interfering peaks due to the excipients present in the pharmaceutical tablets. Thus, the proposed method is simple and suitable for the simultaneous analysis of active ingredients in tablet dosage forms...
  68. ncbi LC-UV method development and validation for the investigational anticancer agent imexon and identification of its degradation products
    Monique W J den Brok
    Slotervaart Hospital The Netherlands Cancer Institute, Department of Pharmacy and Pharmacology, Louwesweg 6, 1066 EC Amsterdam, The Netherlands
    J Pharm Biomed Anal 38:686-94. 2005
    ..The developed LC-UV method was found suitable for the pharmaceutical quality control of imexon API and the drug in its pharmaceutical dosage form...
  69. ncbi Isolation, identification and determination of the major degradation product in alprazolam tablets during their stability assay
    A L Huidobro
    Sección de Química Analítica, Facultad de Farmacia, Universidad San Pablo CEU, Campus Monteprincipe, 28668 Boadilla del Monte, Madrid, Spain
    J Pharm Biomed Anal 44:404-13. 2007
    ..In addition, a LC method has been developed and validated including triazolaminoquinoleine for the adequate determination of alprazolam and its mayor degradation product in tablets as pharmaceutical forms...
  70. ncbi Liquid chromatographic method for quantitation of patulin at 10 ng/mL in apple-based products intended for infants: interlaboratory study
    Isabel Arranz
    Institute for Reference Materials and Measurements, Food Safety and Quality Unit, European Commission Joint Research Centre, Retieseweg 111, B 2440 Geel, Belgium
    J AOAC Int 88:518-25. 2005
    ..5 to 35.2%, reflecting HORRAT values from 0.6 to 1.0 for juice and 0.4 to 0.9 for puree. The method showed acceptable within-laboratory and between-laboratory precision for each matrix, as required by current European legislation...
  71. ncbi One-step liquid chromatographic method for the determination of oxytetracycline in fish muscle
    Rosie Coyne
    Department of Aquaculture, Institute of Marine Research, P O Box 1870, Nordnes, N 5817 Bergen, Norway
    J Chromatogr B Analyt Technol Biomed Life Sci 810:325-8. 2004
    ..The method was successfully applied to a pharmacokinetic study...
  72. ncbi Determination of several N-methyl-D-aspartate receptor blockers in plasma and brain by a selective high-performance liquid chromatographic method with column switching
    R Wyss
    Pharmaceuticals Division, F. Hoffmann-La Roche Ltd, PRNK, Basel, Switzerland
    J Chromatogr A 797:211-20. 1998
    ..The low degree of interference allowed the development of sensitive methods with quantification limits of 5 ng/ml for animal plasma (0.4 ml used), 0.5 ng/ml for human plasma (1 ml used) and 50 ng/g for brain tissue (200 mg used)...
  73. ncbi Alkaline hydrolysis of cladribine
    M Andrzejewska
    Institute of Chemistry, Agricultural University, Warsaw, Poland
    Pharmazie 58:122-4. 2003
    ..The first one was the result of the hydroxyl anion attack, whereas the presence of the other nucleoside has evidenced the existence of hitherto undescribed rearrangement reaction in purine derivatives...
  74. ncbi Rapid and simple method for the analysis of nateglinide in human plasma using HPLC analysis with UV detection
    Steffen Bauer
    Institute of Clinical Pharmacology, University Medical Center Charite, Humboldt University of Berlin, Schumannstr 20 21, 10098 Berlin, Germany
    J Pharm Biomed Anal 31:551-5. 2003
    ..Sample preparation time and analysis time are both short and allow rapid analysis of large sample sets...
  75. ncbi Determination of the antibacterial trovafloxacin by differential-pulse adsorptive stripping voltammetry
    Jose Luis Vilchez
    Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s n, E 18071 Granada, Spain
    J Pharm Biomed Anal 31:465-71. 2003
    ..6 ng ml(-1). The method was applied to determination of TRFLX in human urine and serum samples. It was validated using HPLC as a reference method. Recovery levels of the method reached 100% in all cases..
  76. ncbi Chromatographic determination of clotrimazole, ketoconazole and fluconazole in pharmaceutical formulations
    E M Abdel-Moety
    Faculty of Pharmacy, Cairo University, Kasr El Aini, ET 11562 Cairo, Egypt
    Farmaco 57:931-8. 2002
    ..1, by volumes), CZ was well separated from its acid degradation products and quantified by densitometric scanning at 260 nm...
  77. ncbi A simple high-performance liquid chromatographic method for the determination of acyclovir in human plasma and application to a pharmacokinetic study
    Liyan Yu
    Center for Instrumental Analysis, China Pharmaceutical University, Key Laboratory of Drug Quality Control and Pharmacovigilance under the Ministry of Education, Nanjing People s Republic of China
    Arzneimittelforschung 58:199-202. 2008
    ..90% confidence limits calculated for C(max) and AUC from zero to infinity (AUC(0-infinity)) of acyclovir were included in the bioequivalence range (0.8-1.25 for AUC)...
  78. ncbi Determination of diphenhydramine hydrochloride in some single tertiary alkylamine pharmaceutical preparations by flow injection spectrophotometry
    Prapatsorn Tipparat
    The Institute for Science and Technology Research and Development, and Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
    J Pharm Biomed Anal 30:105-12. 2002
    ..A throughput of 100 injections h(-1) can be obtained. Application to assay for capsule and syrup samples has been demonstrated. The method is validated by comparing the results with HPLC analyses...
  79. ncbi Simultaneous determination of terpene lactones and flavonoid aglycones in Ginkgo biloba by high-performance liquid chromatography with evaporative light scattering detection
    Wenkui Li
    Department of Medicinal Chemistry and Pharmacognosy, Program for Collaborative Research in Pharmaceutical Sciences, University of Illinois at Chicago, 833 South Wood Street, 60612 7231, Chicago, IL, USA
    J Pharm Biomed Anal 30:67-75. 2002
    ..998. The reproducibility of the method was evaluated by analyzing three sets of controls on 3 consecutive days with RSD% and relative errors (RE%) less than 17.26 and 14.67%...
  80. ncbi Development and validation of a HPLC method for the determination of cetirizine in pharmaceutical dosage forms
    B Paw
    Medical University of Lublin, Faculty of Pharmacy, Department of Medicinal Chemistry, Lublin, Poland
    Pharmazie 57:313-5. 2002
    ..The method was validated for linearity, precision, accuracy and limit of detection. The recovery (mean +/- SD) for tablets was 100.88% +/- 0.8967, for drops 100.35% +/- 0.4062 and for solutions 101.20% +/- 1.1698...
  81. ncbi Separation of levofloxacin, ciprofloxacin, gatifloxacin, moxifloxacin, trovafloxacin and cinoxacin by high-performance liquid chromatography: application to levofloxacin determination in human plasma
    Hairui Liang
    Purdue University, School of Pharmacy and Pharmacal Sciences, Department of Pharmacy Practice, Indianapolis, IN, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 772:53-63. 2002
    ..The method was successfully applied to patient plasma samples in support of a levofloxacin pharmacokinetic study...
  82. ncbi LC and TLC determination of cinnarizine in pharmaceutical preparations and serum
    Saad S M Hassan
    Department of Chemistry, Faculty of Science, Ain Shams University, Cairo, Egypt
    J Pharm Biomed Anal 28:711-9. 2002
    ..The results obtained by both techniques are in good agreement and offer the advantages of reproducibility and accuracy...
  83. ncbi LC determination and degradation study of droperidol
    H Trabelsi
    Laboratoire National de Contrôle des Médicaments 11bis, Rue Jebel Lakhdar, Bab Saadoun, 1006 Tunis, Tunisia
    J Pharm Biomed Anal 28:453-62. 2002
    ..The degradation was carried out in hydrochloric acid, sodium hydroxide and hydrogen peroxide. The main degradation products were identified. Application of the assay for a commercial preparation of droperidol for injection is presented...
  84. ncbi Spectrophotometric, spectrofluorimetric, HPLC and CZE determination of mirtazapine in pharmaceutical tablets
    L Labat
    Laboratoire de Chimie Analytique, Faculte de Pharmacie, 3 Place de la Victoire, F 33076 Cedex, Bordeaux, France
    J Pharm Biomed Anal 28:365-71. 2002
    ..54%). The four methods were directly and easily applied to the pharmaceutical preparation with accuracy, resulting from recovery experiments between 99.72% in HPLC and 101.47% in spectrofluorimetry...
  85. ncbi Concentrations of p-synephrine in fruits and leaves of Citrus species (Rutaceae) and the acute toxicity testing of Citrus aurantium extract and p-synephrine
    M D Arbo
    Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de Farmacia, Universidade Federal do Rio Grande do Sul, Av Ipiranga, 2752, CEP 90610 000, Porto Alegre, RS, Brazil
    Food Chem Toxicol 46:2770-5. 2008
    ..All the effects were reversible and persisted for 3-4h. The toxic effects observed seem to be related with adrenergic stimulation and should alert for possible side effects of p-synephrine and C. aurantium...
  86. ncbi Evaluation of a fluorogenic derivatization method for the reversed-phase HPLC analysis of 2'-beta-fluoro-2',3'-dideoxyadenosine, a new anti-AIDS drug
    H Zhang
    Laboratory of Medicinal Chemistry, Division of Basic Sciences, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702-1201, USA
    J Pharm Biomed Anal 25:285-97. 2001
    ..This method has potential for the measurement of F-ddA at low concentration and in limited volume samples from in vivo biological studies...
  87. ncbi Improved high-performance liquid chromatographic determination of amoxicillin in human plasma by means of column switching
    P Muth
    Medical Biological Development, Heumann Pharma, , Germany
    J Chromatogr A 729:259-66. 1996
    ..The method can be used to determine pharmacokinetic parameters of amoxicillin in humans after a single oral dose of 500 mg...
  88. ncbi Stability indicating RP-HPLC method development and validation for oseltamivir API
    Balasubramanian Narasimhan
    Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Haryana, India
    Chem Pharm Bull (Tokyo) 56:413-7. 2008
    ..The proposed method can be used for routine analysis of oseltamivir in quality control laboratories...
  89. ncbi Synthesis of probes with broad pH range fluorescence
    Maciej Adamczyk
    Department of Chemistry D9MD, Abbott Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, 60064 6016, Abbott Park, IL, USA
    Bioorg Med Chem Lett 13:2327-30. 2003
    ....
  90. ncbi Simultaneous identification and quantification of anthraquinones, polydatin, and resveratrol in Polygonum multiflorum, various Polygonum species, and dietary supplements by liquid chromatography and microscopic study of Polygonum species
    Bharathi Avula
    National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA
    J AOAC Int 90:1532-8. 2007
    ..multiflorum. The commercial samples and the 6 Polygonum species were compared microscopically, and a detailed description is provided for P. multiflorum...
  91. ncbi Long-term stability study of L-adrenaline injections: Kinetics of sulfonation and racemization pathways of drug degradation
    David Stepensky
    Research and Quality Control Laboratory, The Medical Corps, Mil P O Box 02149, Israel Defense Forces, Israel Defense Forces, Israel
    J Pharm Sci 93:969-80. 2004
    ..The modeling revealed significant differences in the degradation kinetics in the formulations produced before and after 1997, whose cause remained unidentified in this study...
  92. ncbi Rapid and simple determination of doxycycline in serum by high-performance liquid chromatography. Application to particulate drug delivery systems
    Noelia Ruz
    Departamento de Farmacia y Tecnologia Farmaceutica, Facultad de Farmacia, Universidad de Navarra, C Irunlarrea 1, 31080 Pamplona, Spain
    J Chromatogr A 1031:295-301. 2004
    ..Linearity was confirmed in the concentration range 0.4-80 microg/ml for doxycycline quantification in serum and from 1 to 800 microg/ml for doxycycline extracted from DDS samples...
  93. pmc Determination of ephedrine alkaloids in botanicals and dietary supplements by HPLC-UV: collaborative study
    Mark C Roman
    ChromaDex, 13161 56th Ct, Suite 201, Clearwater, FL 33760, USA
    J AOAC Int 87:1-14. 2004
    ..69-3.23, exclusive of the results from the high protein drink). On the basis of these results, the method is recommended for Official First Action for determination of EP and PS in dietary supplements exclusive of the high protein drinks...
  94. ncbi Spectrophotometric, spectrofluorometric and HPLC determination of desloratadine in dosage forms and human plasma
    Nahed El-Enany
    Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt
    Chem Pharm Bull (Tokyo) 55:1662-70. 2007
    ..The proposed methods are highly specific for determination of DSL in the presence of the parent drug loratadine. A proposal for the reaction pathways is postulated...
  95. ncbi Potassium hexacyanoferrate(III)-mediated fluorometric determination of melatonin
    Hideaki Iizuka
    Faculty of Pharmaceutical Sciences, Toho University 2 2 1 Miyama Funabashi, Chiba 274 8510, Japan
    Adv Exp Med Biol 527:587-92. 2003
    ..This fluorescent reaction was used for the precolumn derivatization of melatonin. Solid phase extraction was used for concentrating melatonin below a level of detection limit...
  96. ncbi Determination of the new HIV-protease inhibitor atazanavir by liquid chromatography after solid-phase extraction
    S Colombo
    Division de pharmacologie clinique, Departement de Medecine, Laboratoire BH 18 218, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, CHUV, Switzerland
    J Chromatogr B Analyt Technol Biomed Life Sci 810:25-34. 2004
    ..4 +/- 3.2%. The method is precise with mean inter-day CVs within 1.1-6.1%, and accurate (range of inter-day deviations +0.3 to +2.3%). The method has been validated and is currently applied to the monitoring of ATV in HIV patients...
  97. ncbi Quantification of a novel retinoic acid metabolism inhibitor, 4-(1H-imidazol-1-yl)retinoic acid (VN/14-1RA) and other retinoids in rat plasma by liquid chromatography with diode-array detection
    Chunyu Wu
    Department of Pharmaceutical Sciences, School of Pharmacy, Temple University, Philadelphia, PA 19140, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 810:203-8. 2004
    ..The mean terminal elimination half-life (t(1/2)) was 19.0 +/- 3.2 min. This HPLC method is useful for the analysis and evaluation of the pharmacokinetics of VN/14-1RA in rats...
  98. ncbi Assay of plasma semicarbazide-sensitive amine oxidase and determination of its endogenous substrate methylamine by liquid chromatography
    Hui Li
    Central Laboratory, Shantou University Medical College, Xin Ling Road 22, Shantou, Guangdong 515041, China
    J Chromatogr B Analyt Technol Biomed Life Sci 810:277-82. 2004
    ..The supernatant was derivatized with dansyl chloride and analyzed by HPLC with fluorescence detection. Recoveries of spiked methylamine at ppb (ng/ml) level were between 93.7 and 97.6% with relative standard deviation less than 2.5%...
  99. ncbi HPLC determination of malondialdehyde in ECV304 cell culture medium for measuring the antioxidant effect of vitexin-4''-O-glucoside
    Xi Xiang Ying
    School of Pharmacy, Liaoning University of TCM, No 79 Chongshan East Road, Shenyang, China
    Arch Pharm Res 31:878-85. 2008
    ..0125 microM. This chromatographic method was successfully applied to investigating the in vitro antioxidant effect of vitexin-4''-O-glucoside. Vitexin-4''-O-glucoside (120 M) protected ECV304 cells from peroxidation induced by TBHP...
  100. ncbi Selective scavenging property of the indole moiety for the nitrating species of peroxynitrite
    Hidehiko Nakagawa
    Redox Regulation Research Group, National Institute of Radiological Sciences, 4 9 1 Anagawa, Inage Ku, Chiba 263 8555, Japan
    Chem Pharm Bull (Tokyo) 52:146-9. 2004
    ..These results imply that the peroxynirite reaction at least partly proceeds through specific species for nitration...
  101. ncbi HPLC method with UV detection for evaluation of digoxin tablet dissolution in acidic medium after solid-phase extraction
    A Jedlicka
    Léciva a s, U kabelovny 130, Dolní Mecholupy, 102 37 Prague 10, Czech Republic
    J Pharm Biomed Anal 33:109-15. 2003
    ..0 mm), as mobile phase water and acetonitrile (72:28, v/v) were used. Detection wavelength was 218 nm. Identity of digoxin degradation products was confirmed by HPLC-MS...

Research Grants15

  1. Synthesis and Evaluation of Nano-antioxidants for Medical Applications
    Choon Lee; Fiscal Year: 2009
    ..Lipid peroxidation will be measured by monitoring diene formation with ultraviolet spectrophotometry while cell damage will be evaluated by red blood cell hemolysis...
  2. Comparative In Vivo Biodistribution of Characterized Manufactured Nanomaterials
    Nancy A Monteiro Riviere; Fiscal Year: 2010
    ..These coordinated studies will provide data to link physical chemical characterizations to in vivo MNM disposition. ..
  3. Cross-Coupling Reactions of Organosilanols
    SCOTT DENMARK; Fiscal Year: 2009
    ..Synthetic approaches to isodomoic acid H, hepatitis C viral inihibiting indole alkaloids, the salicylihalimide macrolactone oximidine II, and polyene antiviral antibiotic viridenomycin are detailed. ..
  4. STRUCTURE AND DYNAMICS OF HEME PROTEIN ACTIVE SITES
    JAMES SATTERLEE; Fiscal Year: 2005
    ..We shall proceed using modern protein engineering methods combined with x-ray crystallography, kinetics, photothermal methods, equillibrium dynamics methods and NMR spectroscopy. ..
  5. Tandem Cycloaddition Chemistry of 2-Azoniaheterodienes
    SCOTT DENMARK; Fiscal Year: 2007
    ..abstract_text> ..
  6. Camptothecin Analogs for Cancer Therapy
    LEE MORGAN; Fiscal Year: 2007
    ..Prior to the latter, DEKK-TEC proposes screening the analogs in a wide spectrum of tumor models to insure the best cancer applications for the products. [unreadable] [unreadable] [unreadable]..
  7. Biomaterials as Decorporation Agents for Radionuclides
    TATIANA LEVITSKAIA; Fiscal Year: 2008
    ..abstract_text> ..
  8. Genetic Studies of Substance Abuse in Iowa Adoptees
    Robert Philibert; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  9. DERIVATIVES OF DEMETHYLPENCLOMEDINE:ANTICANCER AGENTS
    LEE MORGAN; Fiscal Year: 2003
    ..abstract_text> ..
  10. New Ligands for Radioimmunotherapy and MRI of Cancer
    Hyun Soon Chong; Fiscal Year: 2006
    ..The results of the proposed research will contribute to the development of better drugs for both MRI and RIT, which will greatly enhance clinical exploration of both modalities, and directly benefit cancer patients. ..
  11. Proteomic determination of arsenical action
    Partha Basu; Fiscal Year: 2005
    ..These studies will greatly expand the understanding of the biochemical activation of vascular cell signaling that is relevant to arsenic-related vascular diseases. ..
  12. A thyroid receptor co-activator hypothesis for psychosis
    Robert Philibert; Fiscal Year: 2005
    ..The net effect will be to produce an independent investigator capable of functional and translational research. ..
  13. Clinical Development of 4-Hydroperoxyifosfamide
    LEE MORGAN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  14. Mechanism of in-vitro aging
    LYSLE LEWIS; Fiscal Year: 2007
    ..The proposed research will enhance our understanding of events that occur as a consequence of shortened chromosomes and factors that affect the rate of shortening. [unreadable] [unreadable] [unreadable]..
  15. Host Polymorphisms and Uncomplicated Malaria
    Sunil Parikh; Fiscal Year: 2008
    ..Parikh will be able to utilize modern molecular epidemiology techniques to investigate human genetic factors underlying susceptibility to malaria and he will be well equipped for a career in academic research. ..