spectrophotometry

Summary

Summary: The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.

Top Publications

  1. ncbi Biochemical analyses of the electron transport chain complexes by spectrophotometry
    Ann E Frazier
    Murdoch Children s Research Institute, Parkville, Vic, Australia
    Methods Mol Biol 837:49-62. 2012
  2. ncbi Redox and ATP control of photosynthetic cyclic electron flow in Chlamydomonas reinhardtii (I) aerobic conditions
    Jean Alric
    UMR 7141, CNRS et Université Pierre et Marie Curie Paris VI, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie 75005 Paris, France
    Biochim Biophys Acta 1797:44-51. 2010
  3. ncbi Global and target analysis of time-resolved spectra
    Ivo H M van Stokkum
    Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands
    Biochim Biophys Acta 1657:82-104. 2004
  4. ncbi Accuracy of indocyanine green pulse spectrophotometry clearance test for liver function prediction in transplanted patients
    Chung Bao Hsieh
    Department of Surgery, Tri Service General Hospital, National Defense Medical Center, Taipei, Taiwan, China
    World J Gastroenterol 10:2394-6. 2004
  5. ncbi Evaluation of the effect of topical agents on radiation-induced skin disease by reflectance spectrophotometry
    Luisa Rizza
    School of Pharmacy, University of Catania, Italy
    J Pharm Pharmacol 62:779-85. 2010
  6. ncbi Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae
    Sandrine Bernabeu
    Service de Bacteriologie Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hopital de Bicetre, Assistance Publique Hopitaux de Paris, Faculte de Medecine Paris Sud, K Bicêtre, France
    Diagn Microbiol Infect Dis 74:88-90. 2012
  7. ncbi Perioperative real-time monitoring of indocyanine green clearance by pulse spectrophotometry predicts remnant liver functional reserve in resection of hepatocellular carcinoma
    S Ohwada
    Department of Surgery, Graduate School of Medicine, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8511, Japan
    Br J Surg 93:339-46. 2006
  8. ncbi Magnetic nanoparticle labeling of mesenchymal stem cells without transfection agent: cellular behavior and capability of detection with clinical 1.5 T magnetic resonance at the single cell level
    Jong Kai Hsiao
    Department of Medical Imaging, National Taiwan University Hospital, Taipei, Taiwan
    Magn Reson Med 58:717-24. 2007
  9. pmc Oxidative DNA damage and total antioxidant status in glaucoma patients
    Rana Sorkhabi
    Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
    Mol Vis 17:41-6. 2011
  10. pmc Heme transfer from streptococcal cell surface protein Shp to HtsA of transporter HtsABC
    Mengyao Liu
    Veterinary Molecular Biology, Montana State University, P O Box 173610, Bozeman, MT 59717, USA
    Infect Immun 73:5086-92. 2005

Detail Information

Publications327 found, 100 shown here

  1. ncbi Biochemical analyses of the electron transport chain complexes by spectrophotometry
    Ann E Frazier
    Murdoch Children s Research Institute, Parkville, Vic, Australia
    Methods Mol Biol 837:49-62. 2012
    ..Determining the enzymatic activities can be valuable in defining isolated or multicomplex disorders and may be relevant to the design of future molecular investigations...
  2. ncbi Redox and ATP control of photosynthetic cyclic electron flow in Chlamydomonas reinhardtii (I) aerobic conditions
    Jean Alric
    UMR 7141, CNRS et Université Pierre et Marie Curie Paris VI, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie 75005 Paris, France
    Biochim Biophys Acta 1797:44-51. 2010
    ..The present data show that the cyclic electron flow can operate under aerobic conditions and support a simple competition model where the excess reducing power is recycled to match the demand for ATP...
  3. ncbi Global and target analysis of time-resolved spectra
    Ivo H M van Stokkum
    Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands
    Biochim Biophys Acta 1657:82-104. 2004
    ..This spectrotemporal modeling approach is illustrated with an elaborate case study of the ultrafast dynamics of the photoactive yellow protein...
  4. ncbi Accuracy of indocyanine green pulse spectrophotometry clearance test for liver function prediction in transplanted patients
    Chung Bao Hsieh
    Department of Surgery, Tri Service General Hospital, National Defense Medical Center, Taipei, Taiwan, China
    World J Gastroenterol 10:2394-6. 2004
    ..To investigate whether the non-invasive real-time Indocyanine green (ICG) clearance is a sensitive index of liver viability in patients before, during, and after liver transplantation...
  5. ncbi Evaluation of the effect of topical agents on radiation-induced skin disease by reflectance spectrophotometry
    Luisa Rizza
    School of Pharmacy, University of Catania, Italy
    J Pharm Pharmacol 62:779-85. 2010
    ..In the present study we investigated the effects of topical agents used as supportive care to minimise radiation-induced skin disease using an instrumental method...
  6. ncbi Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae
    Sandrine Bernabeu
    Service de Bacteriologie Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hopital de Bicetre, Assistance Publique Hopitaux de Paris, Faculte de Medecine Paris Sud, K Bicêtre, France
    Diagn Microbiol Infect Dis 74:88-90. 2012
    ..They are mostly of the KPC, VIM, IMP, NDM, and OXA-48 type. A spectrophotometry technique based on analysis of the imipenem hydrolysis has been developed that differentiated carbapenemase- ..
  7. ncbi Perioperative real-time monitoring of indocyanine green clearance by pulse spectrophotometry predicts remnant liver functional reserve in resection of hepatocellular carcinoma
    S Ohwada
    Department of Surgery, Graduate School of Medicine, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8511, Japan
    Br J Surg 93:339-46. 2006
    ..There is no standard method for predicting remnant liver functional reserve after hepatectomy or for monitoring it in real time...
  8. ncbi Magnetic nanoparticle labeling of mesenchymal stem cells without transfection agent: cellular behavior and capability of detection with clinical 1.5 T magnetic resonance at the single cell level
    Jong Kai Hsiao
    Department of Medical Imaging, National Taiwan University Hospital, Taipei, Taiwan
    Magn Reson Med 58:717-24. 2007
    ..No significant change was found in viability, proliferation, MMP change, ROS production, or differentiation capacity. About 45.2% of the hMSCs could be detected using 1.5 T MRI at the single cell level with 3D GRE and four repetitions...
  9. pmc Oxidative DNA damage and total antioxidant status in glaucoma patients
    Rana Sorkhabi
    Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
    Mol Vis 17:41-6. 2011
    ..To evaluate DNA damage markers and the antioxidant status of serum and aqueous humor in glaucoma patients...
  10. pmc Heme transfer from streptococcal cell surface protein Shp to HtsA of transporter HtsABC
    Mengyao Liu
    Veterinary Molecular Biology, Montana State University, P O Box 173610, Bozeman, MT 59717, USA
    Infect Immun 73:5086-92. 2005
    ..These findings demonstrate for the first time complex formation and directional heme transfer between a cell surface heme-binding protein and the lipoprotein of a heme-specific ABC transporter in gram-positive bacteria...
  11. ncbi A new quantitative method using glucose for analysis of endodontic leakage
    Qiong Xu
    School of Stomatology, Wuhan University, China
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99:107-11. 2005
    ..The purpose of this study was to introduce a new method for quantitative testing of endodontic leakage...
  12. pmc The conformation of serum albumin in solution: a combined phosphorescence depolarization-hydrodynamic modeling study
    M L Ferrer
    , Consejo Superior de Investigaciones Cientificas, 28006 Madrid, Spain
    Biophys J 80:2422-30. 2001
    ..Therefore, the overall conformation in neutral solution of BSA, as of HSA, should be rigid, in the sense indicated above, and very similar to the heart-shaped structure observed in HSA crystals...
  13. ncbi Spectrophotometric determination of freshwater pH using bromocresol purple and phenol red
    W Yao
    College of Marine Science, University of South Florida, St Petersburg 33701, USA
    Environ Sci Technol 35:1197-201. 2001
    ..5 < or =pH < or =8.5. The precision of pH measurements using phenol red in well-buffered freshwaters is on the order of +/-0.001 or better...
  14. pmc Proton pathways in green fluorescence protein
    Noam Agmon
    Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel
    Biophys J 88:2452-61. 2005
    ....
  15. ncbi The spectral sensitivity of the human short-wavelength sensitive cones derived from thresholds and color matches
    A Stockman
    Department of Psychology, University of California San Diego, La Jolla 92093 0109, USA
    Vision Res 39:2901-27. 1999
    ..At longer wavelengths, S-cone sensitivity has previously been over-estimated...
  16. ncbi Two isoforms of chicken melanopsins show blue light sensitivity
    Masaki Torii
    Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    FEBS Lett 581:5327-31. 2007
    ..The melanopsin isoforms found in this study may function as pineal circadian photoreceptors...
  17. ncbi Automatic differentiation of melanoma from melanocytic nevi with multispectral digital dermoscopy: a feasibility study
    M Elbaum
    Electro Optical Sciences, Inc, Irvington, NY, USA
    J Am Acad Dermatol 44:207-18. 2001
    ..Differentiation of melanoma from melanocytic nevi is difficult even for skin cancer specialists. This motivates interest in computer-assisted analysis of lesion images...
  18. ncbi A nanosilver-based spectrophotometry method for sensitive determination of tartrazine in food samples
    R Sahraei
    Department of Chemistry, ILam University, Ilam, Iran
    Food Chem 138:1239-42. 2013
    ..98% (n=10). The method was successfully applied to the determination of tartrazine in lemon, and papaya-flavoured gelatin, candy, and in fruit syrup...
  19. ncbi A rapid spectrophotometric determination of persulfate anion in ISCO
    Chenju Liang
    Department of Environmental Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan
    Chemosphere 73:1540-3. 2008
    ..This proposed spectrophotometric quantification of persulfate provides a simple and rapid method for evaluation of ISCO effectiveness at a remediation site...
  20. pmc Enzymology below 200 K: the kinetics and thermodynamics of the photochemistry catalyzed by protochlorophyllide oxidoreductase
    Derren J Heyes
    Robert Hill Institute for Photosynthesis and Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    Proc Natl Acad Sci U S A 99:11145-50. 2002
    ....
  21. pmc Molecular mechanism of Thioflavin-T binding to amyloid fibrils
    Matthew Biancalana
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1804:1405-12. 2010
    ..These studies have yielded important insights into amyloid structures and the processes of fibril formation, and they also offer guidance for designing the next generation of amyloid assembly diagnostics, inhibitors, and therapeutics...
  22. ncbi FTIR spectrophotometry, kinetics and adsorption isotherms modeling, ion exchange, and EDX analysis for understanding the mechanism of Cd(2+) and Pb(2+) removal by mango peel waste
    Muhammad Iqbal
    Biotechnology Group, Centre for Environment Protection Studies, PCSIR Laboratories Complex, Ferozepur Road, Lahore 54600, Pakistan
    J Hazard Mater 164:161-71. 2009
    ..The study points to the potential of new use of MPW as an effective sorbent for the removal of Cd(2+) and Pb(2+) from aqueous solution...
  23. ncbi Rapid determination of crocetin esters and picrocrocin from saffron spice (Crocus sativus L.) using UV-visible spectrophotometry for quality control
    Ana M Sanchez
    Catedra de Quimica Agricola, ETSI Agronomos, Universidad Castilla La Mancha, 02071 Albacete, Spain
    J Agric Food Chem 56:3167-75. 2008
    ..These models may enhance quality control in saffron enterprises...
  24. ncbi A causative role for redox cycling of myoglobin and its inhibition by alkalinization in the pathogenesis and treatment of rhabdomyolysis-induced renal failure
    K P Moore
    Joint Department of Medicine, Royal Free and University College Medical School, London NW3 2QG, United Kingdom
    J Biol Chem 273:31731-7. 1998
    ..These data strongly support a causative role for oxidative injury in the renal failure of rhabdomyolysis and suggest that the protective effect of alkalinization may be attributed to inhibition of myoglobin-induced lipid peroxidation...
  25. ncbi A comparison of spectrophotometry and color charts for evaluating total plasma carotenoids in wild birds
    J L Tella
    Department of Biology, University of Saskatchewan, Saskatoon, Canada
    Physiol Zool 71:708-11. 1998
    ..We compared color-chart scores of plasma hue with the actual concentration of plasma carotenoids obtained by spectrophotometry in 356 wild birds from 26 species...
  26. ncbi Rapid measurement of indocyanine green retention by pulse spectrophotometry: a validation study in 70 patients with Child-Pugh A cirrhosis before hepatectomy for hepatocellular carcinoma
    Tan To Cheung
    Department of Surgery, The University of Hong Kong, Hong Kong, China
    Hepatobiliary Pancreat Dis Int 11:267-71. 2012
    ..Traditionally, it is done using spectrophotometry with serial blood sampling...
  27. ncbi Endoscopic reflectance spectrophotometry and visible light spectroscopy in clinical gastrointestinal studies
    Felix W Leung
    Division of Gastroenterology, Research and Medical Services, Sepulveda Ambulatory Care Center, VA Greater Los Angeles Healthcare System, 16111 Plummer Street, North Hills, CA 91343, USA
    Dig Dis Sci 53:1669-77. 2008
    The use of reflectance spectrophotometry (RS) for mucosal hemodynamic measurement relies on the recognition of changes in indexes of mucosal hemoglobin concentration and oxygen saturation...
  28. ncbi A high-throughput screening strategy for nitrile-hydrolyzing enzymes based on ferric hydroxamate spectrophotometry
    Yu Cai He
    Changzhou University, People s Republic of China
    Appl Microbiol Biotechnol 89:817-23. 2011
    ..In this paper, a simple, rapid, and high-throughput screening method based on the ferric hydroxamate spectrophotometry has been proposed...
  29. ncbi Determination of sulfonamide compounds in sewage and river by mixed hemimicelles solid-phase extraction prior to liquid chromatography-spectrophotometry
    Ji Dong Li
    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
    J Chromatogr A 1139:178-84. 2007
    ..investigated for the preconcentration of five sulfonamides from environmental water samples prior to HPLC-spectrophotometry determination in this paper...
  30. ncbi Cutaneous melanin density of Caucasians measured by spectrophotometry and risk of malignant melanoma, basal cell carcinoma, and squamous cell carcinoma of the skin
    Terence Dwyer
    Menzies Centre for Population Health Research, University of Tasmania, Hobart, Tasmania, Australia
    Am J Epidemiol 155:614-21. 2002
    Recent advances have enabled quite accurate estimation by spectrophotometry of the density of cutaneous melanin. The relation between skin cancers and this objective measure of skin phenotype is examined here...
  31. ncbi Determination of total catechins in tea extracts by HPLC and spectrophotometry
    Qiang He
    The Department of Food Science and Engineering, Sichuan University, Chengdu, P R China
    Nat Prod Res 23:93-100. 2009
    ..The two methods can be used for the routine determination of total catechins in catechin-containing products...
  32. ncbi Blue CrO5 assay: a novel spectrophotometric method for the evaluation of the antioxidant and oxidant capacity of various biological substances
    Pavlos S Charalampidis
    Laboratory of Physiology, Faculty of Medicine, University of Ioannina, Ioannina 45110 Greece
    Eur J Med Chem 44:4162-8. 2009
    ..oxidant compound, miscible and relatively stable in polar organic solvents, that can be easily measured by spectrophotometry. Its reduction by known antioxidants, both water- and lipid-soluble (ascorbate and alpha-tocopherol, ..
  33. ncbi Evaluation of transepithelial stromal riboflavin absorption with enhanced riboflavin solution using spectrophotometry
    Tariq A Alhamad
    School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom
    J Cataract Refract Surg 38:884-9. 2012
    ..1%, 15% dextran T500 with trometamol (Tris-[hydroxymethyl]aminomethane) and sodium ethylenediaminetetraacetic acid by analyzing light-transmission properties of ex vivo rabbit corneas...
  34. ncbi Co-aggregation and growth inhibition of probiotic lactobacilli and clinical isolates of mutans streptococci: an in vitro study
    Mette Kirstine Keller
    Institute of Odontology, Department of Cariology and Endodontics, Faculty of Health Sciences, University of Copenhagen, Denmark
    Acta Odontol Scand 69:263-8. 2011
    ..The aim was to investigate the in vitro ability of selected commercial probiotic lactobacilli to co-aggregate and inhibit growth of oral mutans steptococci isolated from adults with contrasting levels of caries...
  35. ncbi Free flap monitoring using simultaneous non-invasive laser Doppler flowmetry and tissue spectrophotometry
    Frank Holzle
    Department of Oral and Maxillofacial Plastic Surgery, Knappschaftskrankenhaus Bochum Langendreer, Ruhr University Bochum, Germany
    J Craniomaxillofac Surg 34:25-33. 2006
    ..The aim of this study was to investigate the benefits of the tissue oxygen analysis system O(2)C for monitoring patients undergoing maxillofacial reconstruction with fasciocutaneous radial forearm flaps...
  36. ncbi Measurement of stain on extracted teeth using spectrophotometry and digital image analysis
    D L Lath
    Department of Oral Health and Development, School of Clinical Dentistry, University of Sheffield, Claremont Crescent, UK
    Int J Dent Hyg 5:174-9. 2007
    ..a customized image analysis system, designed for use within clinical trials of general dental hygiene and whitening products, for the measurement of stain levels on extracted teeth and to compare it with reflectance spectrophotometry.
  37. ncbi Assessment of oxygenation and perfusion in the tongue and oral mucosa by visible spectrophotometry and laser Doppler flowmetry in healthy subjects
    D B Singh
    University Hospital of North Durham, Durham, UK
    Adv Exp Med Biol 614:227-33. 2008
    Use of Visible Light Spectrophotometry (VLS) and Laser Doppler Flowmetry (LDF) is currently being studied by the authors to assess the viability of tissue margins in colon resection and to assess mucosal oxygenation in the colon...
  38. ncbi Electron transfer in human methionine synthase reductase studied by stopped-flow spectrophotometry
    Kirsten R Wolthers
    Department of Biochemistry, University of Leicester, University Road, Leicester, LE1 7RH, United Kingdom
    Biochemistry 43:490-500. 2004
    ..Comparisons are made with similar stopped-flow kinetic studies of the structurally related enzymes cytochrome P450 reductase and nitric oxide synthase...
  39. ncbi Use of reflectance spectrophotometry and colorimetry in a general linear model for the determination of the age of bruises
    Vanessa K Hughes
    School of Arts and Sciences NSW ACU National, North Sydney, Australia
    Forensic Sci Med Pathol 6:275-81. 2010
    ..This study sought to determine if colorimetry or reflectance spectrophotometry could be employed to objectively estimate the age of bruises...
  40. ncbi First-derivative spectrophotometry in the analysis of rosiglitazone in coated tablets
    Patricia Gomes
    Universidade Federal do Rio Grande do Sul, Faculdade de Farmacia, Programa de Pós Graduação em Ciências Farmacêuticas, Porto Alegre, RS, Brazil
    J AOAC Int 89:1296-9. 2006
    First-derivative spectrophotometry, applying the peak-zero method, was developed for the determination of rosiglitazone (RSG) in coated tablets. The solutions of standard and sample were prepared in ethanol...
  41. ncbi Clinical evaluation of reflectance spectrophotometry for the measurement of gastric microvascular oxygen saturation in patients undergoing cardiopulmonary bypass
    Artur Fournell
    Department of Anesthesiology, Heinrich Heine University, Dusseldorf, Germany
    J Cardiothorac Vasc Anesth 16:576-81. 2002
    To evaluate the impact of cardiopulmonary bypass (CPB) on gastric mucosal oxygen saturation assessed by reflectance spectrophotometry in patients undergoing coronary artery bypass graft surgery.
  42. ncbi Utilization of picolinaldehyde-4-phenyl-3-thiosemicarbazone in sodium dodecylsulfate micelles for the spectrophotometric determination of iron, vanadium, and cobalt following partial least-squares regression analysis
    Aamna Balouch
    University of Sindh, Centre of Excellence in Analytical Chemistry, Jamshoro, Pakistan
    J AOAC Int 92:248-56. 2009
    ....
  43. ncbi Stationary cuvette: a new approach to obtaining analytical curves by UV-VIS spectrophotometry
    K G H Silva
    Programa de Pos Graduacao em Ciencias da Saude, Universidade Federal do Rio Grande do Norte, Natal RN, Brazil
    Phytochem Anal 20:265-71. 2009
    ..An analytical curve is one of the most important steps in the chemical analysis presenting a direct relationship to features such as linearity...
  44. pmc Nitric oxide binds to the proximal heme coordination site of the ferrocytochrome c/cardiolipin complex: formation mechanism and dynamics
    Gary Silkstone
    Department of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, United Kingdom
    J Biol Chem 285:19785-92. 2010
    ..These features all point at a remarkable mobility of the proximal heme environment induced by cardiolipin...
  45. ncbi Validation and application of chemometrics-assisted spectrophotometry and liquid chromatography for simultaneous determination of two ternary mixtures containing drotaverine hydrochloride
    Alaa El-Gindy
    Suez Canal University, Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ismailia 41522, Egypt
    J AOAC Int 93:536-48. 2010
    ....
  46. pmc Purification and characterization of meta-cresol purple for spectrophotometric seawater pH measurements
    Xuewu Liu
    College of Marine Science, University of South Florida, 140 Seventh Avenue South, St Petersburg, Florida 33701, USA
    Environ Sci Technol 45:4862-8. 2011
    ..0436 × 10(-4) (S – 35). The mCP absorbances, λ1A and λ2A, used to calculate R are measured at wavelengths (λ) of 434 and 578 nm. This characterization is appropriate for 278.15 ≤ T ≤ 308.15 and 20 ≤ S ≤ 40...
  47. ncbi Spectroscopic properties of the main-form and high-salt peridinin-chlorophyll a proteins from Amphidinium carterae
    Robielyn P Ilagan
    Department of Chemistry, 55 North Eagleville Road, University of Connecticut, Storrs, Connecticut 06269 3060, USA
    Biochemistry 43:1478-87. 2004
    ..The study provides insight into the molecular factors that control energy transfer in this class of light-harvesting pigment-protein complexes...
  48. ncbi The determination and quantification of photosynthetic pigments by reverse phase high-performance liquid chromatography, thin-layer chromatography, and spectrophotometry
    Tessa Pocock
    Department of Biology, The University of Western Ontario, London, Canada
    Methods Mol Biol 274:137-48. 2004
    ..In addition, several spectrophotometric methods for the quantification of chlorophylls a and b are described...
  49. ncbi An investigation of microleakage from root-end fillings in ultrasonic retrograde cavities with or without finishing: a quantitative analysis
    Eudes Gondim
    Endodontic Unit, School of Dentistry of Piracicaba, State University of Campinas UNICAMP Piracicaba SP, Brazil
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99:755-60. 2005
    ..The aim of this study was to make a quantitative assessment of the sealing ability of Super-EBA, IRM, and Pro Root MTA root-end fillings subjected to 3 different finishing techniques...
  50. ncbi Improved fluorometric quantification of urinary xanthurenic acid
    M Liu
    Department of Nutritional Sciences, University of California, Berkeley, 94720, USA
    Clin Chem 42:397-401. 1996
    ..The proposed method for urinary XA is rapid, simple, and suitable for routine use in the clinical laboratory...
  51. ncbi Profiling of normal human leg lymph proteins using the 2-D electrophoresis and SELDI-TOF mass spectrophotometry approach
    B Interewicz
    Department of Surgical Research and Transplantology, Medical Research Center, Polish Academy of Sciences, Warsaw, Poland
    Lymphology 37:65-72. 2004
    ..w. proteins. They challenge the commonly accepted notion of unlimited free diffusion of peptides across the capillary membrane...
  52. ncbi Color stability of dry earth pigmented maxillofacial silicone A-2186 subjected to microwave energy exposure
    Sudarat Kiat-Amnuay
    Department of Restorative Dentistry and Biomaterials, The University of Texas Dental Branch at Houston, Houston, TX 77030, USA
    J Prosthodont 14:91-6. 2005
    ..5-year period of microwave sterilization...
  53. ncbi Spectral analysis of cytochromes in rat heart myocytes: transient and steady-state photodiode array spectrophotometry measurements
    P Sarti
    Department of Biochemical Sciences, University of Rome La Sapienza, Italy
    Arch Biochem Biophys 299:8-14. 1992
    ..Spectral analysis was improved by adding carbon monoxide at concentrations which did not affect cytochrome c oxidase activity, but kept myoglobin fully saturated (and thus uninfluential to absorbance changes)...
  54. ncbi Femtosecond transient absorption study of carotenoid to chlorophyll energy transfer in the light-harvesting complex II of photosystem II
    J P Connelly
    Max Planck Institut für Strahlenchemie, Mulheim an der Ruhr, Germany
    Biochemistry 36:281-7. 1997
    ..The results imply predominant energy transfer from the central Ls to Chl b which requires a modification of the currently accepted arrangement of Chl pigments in LHC II...
  55. ncbi Determination of irbesartan in the presence of hydrochlorothiazide by derivative spectrophotometry
    Isabel Albero
    Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain
    J Pharm Biomed Anal 29:299-305. 2002
    ..The calibration graph was linear over the range 2.0-50.0 mg l(-1) of HCT, detection limit was 0.25 mg l(-1). The proposed methods were successfully applied to the determinations of IRB and HCT in combined tablets...
  56. ncbi Detecting Hg2+ ions with an ICT fluorescent sensor molecule: remarkable emission spectra shift and unique selectivity
    Jiaobing Wang
    State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China
    J Org Chem 71:4308-11. 2006
    ..This sensor, employing the ICT mechanism, could be used to specifically detect Hg2+ ions in a neutral buffered water solution with an approximately 100-nm blue shift in emission spectra...
  57. ncbi The excited-state chemistry of protochlorophyllide a: a time-resolved fluorescence study
    Benjamin Dietzek
    Institut fur Physikalische Chemie, Bayerische Julius Maximilians Universität Würzburg, Am Hubland, 97074 Wurzburg, Germany
    Chemphyschem 7:1727-33. 2006
    ....
  58. pmc Spectral magnitude effects on the analyses of secondary structure from circular dichroism spectroscopic data
    Andrew J Miles
    Department of Crystallography, Birkbeck College, University of London, London, UK
    Protein Sci 14:368-74. 2005
    ....
  59. ncbi Copper can promote oxidation of LDL by markedly different mechanisms
    O Ziouzenkova
    Institute of Biochemistry, University of Graz, Austria
    Free Radic Biol Med 24:607-23. 1998
    ....
  60. ncbi Spectrophotometric measurements of pH in-situ: laboratory and field evaluations of instrumental performance
    Xuewu Liu
    College of Marine Science, University of South Florida St Petersburg, 33701 5016, USA
    Environ Sci Technol 40:5036-44. 2006
    ..5 Hz. This work indicates that in-situ instrumentation can be used to provide accurate, precise, and highly resolved observations of carbon-system transformations in the natural environment...
  61. ncbi Phosphatase and oxygen radical-generating activities of mammalian purple acid phosphatase are functionally independent
    Helena Kaija
    Biocenter Oulu, Research Center for Molecular Endocrinology, Oulu University Hospital, University of Oulu, FIN 90014 Finland
    Biochem Biophys Res Commun 292:128-32. 2002
    ..Substrate specificity was not affected by the mutations. These results suggest that AcP and ROS-generating activities of TRAP are functionally independent...
  62. ncbi Targeted misexpression of a Drosophila opsin gene leads to altered visual function
    R Feiler
    Max Planck Institut für biologische Kybernetik, Tubingen, FRG
    Nature 333:737-41. 1988
    ..The photoreceptor cells in which this opsin was misexpressed showed new spectral characteristics and physiology...
  63. ncbi Aneurysmal subarachnoid leak with normal CT and CSF spectrophotometry
    Mark O McCarron
    Department of Neurology, Altnagelvin Hospital, Londonderry, BT47 6SB, UK
    Neurology 64:923. 2005
  64. ncbi Nitric oxide ejects electrons from the binuclear centre of cytochrome c oxidase by reacting with oxidised copper: a general mechanism for the interaction of copper proteins with nitric oxide?
    C E Cooper
    Department of Biological Sciences, University of Essex, Central Campus, Colchester, UK
    FEBS Lett 414:281-4. 1997
    ..These reactions may have implications for the interactions of NO with other copper proteins...
  65. ncbi The radical and redox chemistry of myoglobin and hemoglobin: from in vitro studies to human pathology
    Brandon J Reeder
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, UK
    Antioxid Redox Signal 6:954-66. 2004
    ....
  66. pmc CSF spectrophotometry in the diagnosis of subarachnoid haemorrhage
    S A Iversen
    J Clin Pathol 55:640. 2002
  67. pmc Silver/poly (lactic acid) nanocomposites: preparation, characterization, and antibacterial activity
    Kamyar Shameli
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
    Int J Nanomedicine 5:573-9. 2010
    ..Thus, Ag/PLA-NC films can be used as an antibacterial scaffold for tissue engineering and medical application...
  68. ncbi Decreased glutathione levels and antioxidant enzyme activities in untreated and treated schizophrenic patients
    Monia Raffa
    Biophysical Department, Faculty of Medicine, Research Laboratory of Trace elements, Free Radicals and Antioxidants, University of Monastir, 5000 Monastir, Tunisia
    Prog Neuropsychopharmacol Biol Psychiatry 33:1178-83. 2009
    ....
  69. ncbi Comparison of the accuracy of noninvasive hemoglobin monitoring by spectrophotometry (SpHb) and HemoCue® with automated laboratory hemoglobin measurement
    Lionel Lamhaut
    Department of Anesthesiology and Intensive Care, Hopital Necker Enfants Malades, Assistance Publique Hopitaux de Paris, and Universite Paris Descartes, Paris, France
    Anesthesiology 115:548-54. 2011
    ..such as the HemoCue® 201+ (HemoCue, Ängelholm, Sweden) provide immediate hemoglobin values, a noninvasive, spectrophotometry-based technology (Radical-7®; Masimo Corp...
  70. ncbi A double-blind randomized clinical trial of two carbamide peroxide tooth bleaching agents: 2-year follow-up
    S S Meireles
    Department of Operative Dentistry, Federal University of Paraiba, Joao Pessoa, PB, Brazil
    J Dent 38:956-63. 2010
    ..This double-blind randomized clinical trial aimed to evaluate the whitening effect of two at-home tooth bleaching agents and the effect of dietary habits after 2 years. The patients' view about bleaching longevity was also investigated...
  71. pmc Single chain fragment variable recombinant antibody functionalized gold nanoparticles for a highly sensitive colorimetric immunoassay
    Yang Liu
    Department of Chemistry, Oakland University, Rochester, MI, United States
    Biosens Bioelectron 24:2853-7. 2009
    ..With the advantages of speed, simplicity and specificity, the colorimetric immunoassay based on the functionalized scFv stabilized gold NPs represents a promising approach for protein analysis and clinical diagnostics...
  72. pmc Cerebral hemodynamics in preterm infants during positional intervention measured with diffuse correlation spectroscopy and transcranial Doppler ultrasound
    Erin M Buckley
    Department of Physics and Astronomy, University of Pennsylvania, 209 S 33rd St, Philadelphia, PA 19104, USA
    Opt Express 17:12571-81. 2009
    ....
  73. ncbi Effect ofartificial accelerated aging on color stability and surface roughness of indirect composites
    Fabíola Rejane Zanin
    Departmento de Materiais Dentários e Prótese FORP USP, Sao Paulo, Brasil
    Eur J Prosthodont Restor Dent 16:10-4. 2008
    ..Subsequently, the first measurements for roughness tests and colorimetric spectrophotometry (CIE L*a*b* scale) were performed. Specimens were subject to accelerated aging for 384 hours...
  74. ncbi Color change of vital teeth exposed to bleaching performed with and without supplementary light
    Joe C Ontiveros
    Department of Restorative Dentistry and Biomaterials, The University of Texas Dental Branch at Houston, Houston, TX 77030, USA
    J Dent 37:840-7. 2009
    ..The aim of this clinical study was to evaluate tooth color change after exposure to 25% hydrogen peroxide in-office tooth whitening system, with and without supplementary light exposure...
  75. ncbi Bedside monitoring of circulating blood volume after subarachnoid hemorrhage
    Hidetoshi Kasuya
    Department of Neurosurgery, Tokyo Women s Medical University, Japan
    Stroke 34:956-60. 2003
    ..The purpose of this study was to investigate the circulating blood volume (CBV) after SAH with the use of indocyanine green pulse spectrophotometry.
  76. ncbi Efficacy and safety of 10% and 16% carbamide peroxide tooth-whitening gels: a randomized clinical trial
    Sonia Saeger Meireles
    Federal University of Pelotas, Pelotas, RS, Brazil
    Oper Dent 33:606-12. 2008
    ..09) when the degree of tooth sensitivity between groups was compared. Both 10% and 16% carbamide peroxide concentrations were equally effective and safe for a three-week at-home tooth-bleaching treatment...
  77. ncbi Serine hydroxymethyltransferase from Plasmodium vivax is different in substrate specificity from its homologues
    Kittipat Sopitthummakhun
    Department of Biochemistry and Center for Excellence in Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok, Thailand
    FEBS J 276:4023-36. 2009
    ..The substrate specificity difference between PvSHMT and the mammalian enzyme indicates the dissimilarity between their active sites, which could be exploited for the development of specific inhibitors against PvSHMT...
  78. ncbi Effect of thermocycling on optical parameters of resin composites by the brand and shade
    Seung Hun Lee
    Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea
    Am J Dent 21:361-7. 2008
    ....
  79. pmc Physical methods for investigating structural colours in biological systems
    P Vukusic
    School of Physics, University of Exeter, Exeter EX4 4QL, UK
    J R Soc Interface 6:S133-48. 2009
    ..Finally, we describe the recent developments in the research field of photonic crystals and the implications for the further study of structural coloration in animals...
  80. ncbi Quantitative analysis of rabeprazole sodium in commercial dosage forms by spectrophotometry
    Nafisur Rahman
    Department of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh, India
    Chem Pharm Bull (Tokyo) 56:995-1001. 2008
    ..38 and 0.75 microg ml(-1), respectively. Both methods have been applied successfully for the estimation of rabeprazole sodium in commercial dosage forms. The results are compared with the reference UV spectrophotometric method...
  81. ncbi Oxidation process of bovine heart ubiquinol-cytochrome c reductase as studied by stopped-flow rapid-scan spectrophotometry and simulations based on the mechanistic Q cycle model
    Y Orii
    Department of Public Health, Graduate School of Medicine, Kyoto University, Kyoto 606, Japan
    J Biol Chem 272:17594-604. 1997
    Stopped-flow rapid-scan spectrophotometry was employed to study complicated oxidation processes of ubiquinol-cytochrome c reductase (QCR) that was purified from bovine heart mitochondria and maximally contained 0...
  82. ncbi A greener and highly sensitive flow-based procedure for carbaryl determination exploiting long pathlength spectrophotometry and photochemical waste degradation
    Wanessa R Melchert
    Instituto de Quimica, Universidade de Sao Paulo, 05513 970, PO Box 26077, Sao Paulo, SP, Brazil
    Talanta 81:327-33. 2010
    ..In addition, the residue after treatment was not toxic for Vibrio fischeri bacteria...
  83. ncbi Estimation of pioglitazone hydrochloride and metformin hydrochloride in tablets by derivative spectrophotometry and liquid chromatographic methods
    Madhira B Shankar
    A R College of Pharmacy, Vallabh Vidyanagar 388 120, Gujarat, India
    J AOAC Int 88:1167-72. 2005
    ..PIO) and mefformin hydrochloride (MET) in combined dosage forms were developed using second-derivative spectrophotometry and reversed-phase liquid chromatography (LC)...
  84. ncbi Determination of glucosinolates in 19 Chinese medicinal plants with spectrophotometry and high-pressure liquid chromatography
    Ye Hu
    State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 100029 Beijing, P R China
    Nat Prod Res 24:1195-205. 2010
    ..The total glucosinolate contents were determined by spectrophotometry. Results showed that the high contents of total glucosinolates were found in some herbs of Brassicaceae, ..
  85. ncbi On-line coupling of solid-phase extraction, derivatization reaction and spectrophotometry by sequential injection analysis: application to trifluoperazine assay in human urine
    Abubakr M Idris
    Department of Chemistry, King Faisal University, P O BOX 400, Hofuf 31982, Saudi Arabia
    J Pharmacol Toxicol Methods 56:330-5. 2007
    ..The method was applied for the assay of trifluoperazine in human urine...
  86. pmc Green synthesis of silver/montmorillonite/chitosan bionanocomposites using the UV irradiation method and evaluation of antibacterial activity
    Kamyar Shameli
    Department of Chemistry, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    Int J Nanomedicine 5:875-87. 2010
    ..These results show that Ag/MMT/Cts BNCs can be useful in different biologic research and biomedical applications, such as surgical devices and drug delivery vehicles...
  87. ncbi Effects of prolonged compression on the variations of haemoglobin oxygenation - assessment by spectral analysis of reflectance spectrophotometry signals
    Zengyong Li
    Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, People s Republic of China
    Phys Med Biol 51:5707-18. 2006
    ..The modification of vessel wall oxygen consumption might substantially affect the available oxygen supply to the compressed tissue. This mechanism might be involved in the process leading to pressure ulcer formation...
  88. ncbi Reaction of Desulfovibrio vulgaris two-iron superoxide reductase with superoxide: insights from stopped-flow spectrophotometry
    Victor W Huang
    Department of Chemistry, University of Texas at San Antonio, San Antonio, Texas 78249, USA
    Biochemistry 46:11342-51. 2007
    ....
  89. ncbi Investigation of diffuse correlation spectroscopy in multi-layered media including the human head
    Louis Gagnon
    Institut de Génie Biomédical, Ecole Polytechnique de Montreal, C P 6079, Succ Centre Ville, Montreal, QC, H3C 3A7, Canada
    Opt Express 16:15514-30. 2008
    ..Experimental measurements with a two-layered dynamical phantom confirm the ability of the two-layered analytical model to distinguish flow increase in each layer...
  90. ncbi Rapid biological synthesis of silver nanoparticles using plant leaf extracts
    Jae Yong Song
    Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea
    Bioprocess Biosyst Eng 32:79-84. 2009
    ....
  91. ncbi Simultaneous determination of domperidone and cinnarizine in a binary mixture using derivative spectrophotometry, partial least squares and principle component regression calibration
    Maissa Y Salem
    Department of Analytical Chemistry, Faculty of Pharmacy, Kasr El Aini, 11562, Cairo University, Egypt
    Anal Bioanal Chem 375:211-6. 2003
    ..The first method is the application of derivative spectrophotometry where the linearity range and percentage recoveries for DOM and CINN were 2...
  92. ncbi Evaluation of the MoleMate training program for assessment of suspicious pigmented lesions in primary care
    Annabel Wood
    GP Registrar, Cambridge Vocational Training Scheme, UK
    Inform Prim Care 16:41-50. 2008
    ..The MoleMate training program is a short, computer-based course developed to train primary care practitioners to operate the MoleMate diagnostic tool...
  93. ncbi The evaluation of a novel method comparing quantitative light-induced fluorescence (QLF) with spectrophotometry to assess staining and bleaching of teeth
    A A Adeyemi
    School of Dental Sciences, The University of Liverpool, The Edwards Building, Daulby Street, Liverpool, UK
    Clin Oral Investig 14:19-25. 2010
    ..Stain development/whitening was monitored with QLF (Inspektor Research systems, Amsterdam, Netherlands) and spectrophotometry (Easy shade, Vita Zahnfabrik, Bad Säckingen, Germany)...
  94. ncbi Intraexaminer reliability of measurement of tooth color by spectrophotometry
    Alexander J Hassel
    Poliklinik fur Zahnarztliche Prothetik, Heidelberg, Germany
    Quintessence Int 40:421-6. 2009
    ..To evaluate intraexaminer reliability in measurement of basic tooth color by use of a clinically applicable spectrophotometer...
  95. ncbi In vivo evaluation of melanoma thickness by multispectral imaging and an artificial neural network. A retrospective study on 250 cases of cutaneous melanoma
    Renato Marchesini
    Medical Physics Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy
    Tumori 93:170-7. 2007
    ..Hence, it is reasonable to assume that the same features might be useful to predict melanoma thickness...
  96. ncbi Reactions of dimethylsulfoxide reductase from Rhodobacter capsulatus with dimethyl sulfide and with dimethyl sulfoxide: complexities revealed by conventional and stopped-flow spectrophotometry
    B Adams
    School of Biological Sciences, University of Sussex, Brighton, U K
    Biochemistry 38:8501-11. 1999
    ..Maximum activity was observed at pH 6.5 and below and at 8.3, respectively. Rapid-scan stopped-flow spectrophotometry has been used to investigate the reduction of the enzyme by DMS to a species previously characterized by its ..
  97. ncbi Spectrophotometric intracutaneous analysis versus dermoscopy for the diagnosis of pigmented skin lesions: prospective, double-blind study in a secondary reference centre
    Martin Glud
    Department of Plastic Surgery and Burn Unit, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    Melanoma Res 19:176-9. 2009
    ..However, as the SIAscope in addition to the SIAgraph images produces dermoscopic images, it holds the advantages in training and archiving...
  98. ncbi Application of spectrophotometry to evaluate the concentration of purified White Spot Syndrome Virus
    Qing Zhou
    State Key Laboratory of Virology, Section of Molecular Virology, College of Life Sciences, Wuhan University, Wuhan 430072, PR China
    J Virol Methods 146:288-92. 2007
    ..34x10(8)virions/microl) was obtained and a formula was established: C (virions/microl)=fOD(600)=3.34x10(8)xOD(600), which can be conveniently used to convert the optical density of purified WSSV preparation into the virion concentration...
  99. ncbi Assessment of nurse-led screening of pigmented lesions using SIAscope
    K Govindan
    Department of Plastic and Reconstructive Surgery, Frenchay Hospital, Frenchay, Bristol, UK
    J Plast Reconstr Aesthet Surg 60:639-45. 2007
    ..The sensitivity and specificity of the general practitioner in diagnosing melanoma remains poor...
  100. ncbi The use of a spectrophotometric intracutaneous analysis device in the real-time diagnosis of melanoma in the setting of a melanoma screening clinic
    M A Haniffa
    Department of Dermatology and Regional Medical Physics Department, Royal Victoria Infirmary, Newcastle upon Tyne, UK
    Br J Dermatol 156:1350-2. 2007
    ..Skin imaging devices to aid melanoma diagnosis have been developed in recent years but few have been assessed clinically...
  101. ncbi Measurement of blood volume using indocyanine green measured with pulse-spectrophotometry: its reproducibility and reliability
    Y L He
    Department of Anesthesiology, Osaka University Medical School, Suita City, Japan
    Crit Care Med 26:1446-51. 1998
    To systematically investigate the reproducibility and reliability of a newly developed, less invasive approach of estimating blood volume (BV), using indocyanine green (ICG) measured with pulse-spectrophotometry.

Research Grants62

  1. BONE MATRIX AND BONE RESORPTION
    Paul R Odgren; Fiscal Year: 2012
    ..microscopy, IP and pull-down assays, microtubule disruption, protein crosslinking and high-sensitivity mass spectrophotometry. We will compare resorbing and non-resorbing OCs and wild type vs. ia ra OCs. Under SA1...
  2. Local Vasoconstriction in Postural Tachycardia Syndrome
    Julian M Stewart; Fiscal Year: 2013
    ..using intracatheter reactions of superoxide and peroxynitrite while H2O2 is assessed using fluorescent spectrophotometry. We will determine if sodium ascorbate and losartan improve cutaneous NO and will correlate skin responses ..
  3. Optical spectroscopy to study mitochondrial function within replicating cells
    Roger J Springett; Fiscal Year: 2010
    ..Two-wavelength spectrophotometry has been used extensively to study the cytochromes of the electron transport chain from isolated ..
  4. The regulation of ciliary motility by an axonemal PP2A phosphatase
    Candice Elam; Fiscal Year: 2011
    ..For aim 2, using immunoprecipitation, chemical crosslinking, and mass spectrophotometry, I will test the hypothesis that the B-subunit, in addition to binding the A and C-subunits, interacts with ..
  5. The role of Vitamin D metabolism in Non-Small Cell Lung Cancer
    Nithya Ramnath; Fiscal Year: 2012
    ..For the first aim, we will use proliferation assays, apoptotic markers, mass spectrophotometry and CYP24A1 blocking agents in cell lines that have either high or low CYP24A1...
  6. Role of Deiodinases in Thyroid Hormone Homeostasis
    Ann M Zavacki; Fiscal Year: 2010
    ..will isolate D3 interacting proteins using 2 dimensional differential gel electrophoresis combined with mass spectrophotometry (2-D DICE MS) and the yeast two-hybrid system, with the goal of linking D3 cell biology to physiology...
  7. Role of nucleolin in regulating mRNA stability during DNA damage response (DDR)
    ANJANA DEEPAK SAXENA; Fiscal Year: 2013
    ..We will use mass-spectrophotometry analyses to identify proteins that differentially associate with nucleolin phospho-variants...
  8. Characterizing a novel hemophore from pathogenic and non-pathogenic mycobacteria.
    LISA M MCMATH; Fiscal Year: 2010
    ..of the two hemophores, where heme transfer kinetics between proteins will be observed using stopped-flow spectrophotometry. We will also utilize chromatography methods to observe potential protein-protein interactions between heme ..
  9. Midcareer Investigator Award in Patient-Oriented Research (k24)
    Patricia L Hibberd; Fiscal Year: 2013
    ..placebo, by studying urinary protein profiles using mass spectrophotometry, with Harvard CTSA collaborators...
  10. Chemosensory receptors and the basis of specificity
    STEVEN MUNGER; Fiscal Year: 2009
    ..pockets, in the presence and absence of ligands, will be solved by a combination of circular dichroism spectrophotometry and X-ray crystallography of T1R N-terminal domains...
  11. Catalysis of Isoniazid action by M. tuberculosis catalase-peroxidase
    Richard S Magliozzo; Fiscal Year: 2010
    ..The research will use rapid kinetics measurements based on optical stopped-flow spectrophotometry and rapid freeze-quench electron paramagnetic resonance spectroscopy, and will also apply isothermal ..
  12. Development of Infection-Resistant Suture Materials
    Matthew Phaneuf; Fiscal Year: 2004
    ..of the antibiotic-dyed suture materials, 3) determine antibiotic release under static/washing conditions via spectrophotometry and 4) examine antimicrobial activity of static and washed antibiotic-dyed suture materials...
  13. ORAL CONTROLLED-RELEASE DELIVERY SYSTEMS
    H Rawls; Fiscal Year: 1991
    ..Release will be followed by UV/visible spectrophotometry. The combinations will be varied to produce a wide range of release rates...
  14. CATALYTIC MECHANISM OF CARBONIC ANHYDRASE
    David N Silverman; Fiscal Year: 2012
    ..This provides a range of catalytic activities, geometries, and active-site environments. Stopped-flow spectrophotometry, 18O exchange between CO2 and water measured by mass spectrometry, and solvent H/D isotope effects will be ..
  15. Effects of Natural Agents with Fluoride on Caries
    Hyun Koo; Fiscal Year: 2004
    ..will be determined using a fluoride-selective electrode, calcium will be analyzed by atomic absorption spectrophotometry and phosphorus will be determined colorimetrically...
  16. Synthesis and Evaluation of Nano-antioxidants for Medical Applications
    Choon Lee; Fiscal Year: 2009
    ..Lipid peroxidation will be measured by monitoring diene formation with ultraviolet spectrophotometry while cell damage will be evaluated by red blood cell hemolysis...
  17. MECHANISM OF ACTION OF NEW ANTICANCER AGENTS
    WILLIAM CREASEY; Fiscal Year: 1980
    ..and metabolism of drugs will be studied by high-pressure liquid chromatography, fluorometry, and spectrophotometry. Changes in the function and integrity of the cell membrane will be assayed by transport of metabolites and ..
  18. URINALYSIS FOR PATIENT COMPLIANCE TO CAM THERAPY
    Rama Ramanujam; Fiscal Year: 2006
    ..We believe that this technology will enhance and complement existing HPLC/mass spectrophotometry techniques in the accurate assessment of the use of CAM agents-before during and post clinical trials...
  19. TRACE ELEMENT NUTRITION OF THE LENS--ZINC
    J Bentley; Fiscal Year: 1990
    ..Zinc will be assayed by atomic absorption spectrophotometry and the use of the isotope zinc-65...
  20. Prostaglandin 19-& 20-Hydroxylation by Cytochrome P450
    Bettie Sue Masters; Fiscal Year: 2005
    ..binding constants and rates of binding of various substrates to the CYP4As will be examined by stopped-flow spectrophotometry in the absence and presence of NADPHcytochrome P450 reductase and/or cytochrome b5...
  21. RAPID KINETIC AND OTHER STUDIES OF BIOLOGICAL OXYGENASES
    David Ballou; Fiscal Year: 2002
    ..The proposed study will employ rapid kinetics spectrophotometry, chemical quenching, and other enzymological methods...
  22. DRYING OF THE OCULAR SURFACE
    JEFFREY GILBARD; Fiscal Year: 1992
    ..Tear electrolytes will be measured in tear microvolumes using flameless atomic absorption spectrophotometry. To supplement tear fluid in these diseases, electrolyte solutions will be applied by continuous infusion ..
  23. NOVEL SIGMA RECEPTOR LIGANDS IN NEURODEGENERATION
    Raymond Booth; Fiscal Year: 2000
    ..accumulation of neurotoxic quinone metabolites will be documented in specified brain regions by UV/Vis spectrophotometry. Nerve terminal integrity will be assessed by quantitative autoradiographic assessment of catecholamine ..
  24. DISSOLUTION OF MERCURY FROM DENTAL AMALGAMS
    Miroslav Marek; Fiscal Year: 2001
    ..It will involve in situ electrochemical determination of the oxide thickness, cold- vapor atomic absorption spectrophotometry for dissolved mercury analysis, and various electrochemical techniques to characterize the behavior of the ..
  25. Diagnosis of Cardiac Viability by Functional Ultrasound
    Marek Belohlavek; Fiscal Year: 2005
    ..and C) correlating the MVG data to high-energy phosphate levels in cardiac biopsies analyzed by spectrophotometry. This proposal is designed to study a novel principle of myocardial aberrations reflecting preserved myocyte ..
  26. STRUCTURAL DETERMINANTS OF FAD and FMN-REQUIRING ENZYMES
    Bettie Sue Masters; Fiscal Year: 2005
    ..Jung-Ja P. Kim, Medical College of Wisconsin. Techniques will include state and stopped-flow spectrophotometry, surface plasmon resonance, x-ray crystallography, redox potential studies, and NMR to examine mechanism...
  27. FUNCTION OF HYDROXYLASES AND OTHER OXIDATIVE
    Minor Coon; Fiscal Year: 1993
    ..transfer to or from b-5 by the Fe-II-0-2 complexes of the P-450 isozymes, will be studied by stopped flow spectrophotometry. Preliminary results indicate that b-5 reacts differently with different P-450's and controls the rate of ..
  28. OXIDATION INDUCED MEMBRANE DAMAGE IN CATARACTOGENESIS
    KAILASH BHUYAN; Fiscal Year: 1999
    ..oxidant species such as conjugated dienes, LOOH, malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), by UV spectrophotometry and HPLC in normal lenses of human donor eyes as a function of age...
  29. Infection Resistant Prostethic Valve Sewing Cuffs
    DONALD DEMPSEY; Fiscal Year: 2001
    ..characterize the physical properties of Cipro-dyed Dacron, determine antibiotic release over time via spectrophotometry, examine in vitro antimicrobial properties and assess antibiotic release pharmacokinetics under simulated ..
  30. REACTIVE OXYGEN SPECIES AND HEMOGLOBIN-INDUCED INJURY
    ANN BALDWIN; Fiscal Year: 2003
    ..damage, and on formation of products of lipid peroxidation will be evaluated using electron microscopy and spectrophotometry, respectively...
  31. ALTERED METABOLISM AND CARCINOGENESIS BY DES
    CORAL LAMARTINIERE; Fiscal Year: 1991
    ..High pressure liquid chromatography (HPLC) and spectrophotometry will be used for measuring metabolic end products and aflatoxin-DNA adducts...
  32. SITE SPECIFIC MUTAGENESIS OF H PYLORI CARBONIC ANHYDRASE
    ROGER ROWLETT; Fiscal Year: 1999
    ..The enzymes will be functionally characterized by stopped-flow spectrophotometry and 13C-NMR exchange kinetics in order to identify the rate-determining step(s) in the mechanism of action, ..
  33. HR-MAS-pathologic correlation of prostate tissue markers
    Mark Swanson; Fiscal Year: 2006
    ..of zinc changes on citrate metabolism by assaying zinc levels in prostate tissues by atomic absorption spectrophotometry and zinc transporter gene expression using real-time reverse-transcriptase polymerase chain reaction ..
  34. Triazolopyrimidine Coated Endovascular Stent
    WILLIAM BRODBECK; Fiscal Year: 2005
    ..a mechanism for controlled release; (2) to determine the release kinetics of triazolopyrimidine in vitro by spectrophotometry and HPLC methodologies; (3) to determine in vitro and in vivo biocompatibility and efficacy of ..
  35. Evaluation of Air Samplers with Field Analysis for Lead
    Martin Harper; Fiscal Year: 2001
    ..XRF device, and confirmation analysis in the laboratory by wet chemical digestion and atomic absorption spectrophotometry. If a particular inhalable sampler is found to give results that are sufficiently accurate and precise when ..
  36. A High Brightness Light Source for UV-VIS Spectroscopic Imaging
    STEPHEN HORNE; Fiscal Year: 2010
    ..both generally in optical brightness and in particular in the deep UV region, where high-resolution imaging spectrophotometry shows the most potential value...
  37. Skin Savvy Study
    Carolyn Heckman; Fiscal Year: 2009
    ..cancer prevention), 3) objective skin color changes will be measured using state-of-the-art skin reflectance spectrophotometry, and 4) the proposed studies will test aspects of several major psychological theories (e.g...
  38. Catalysis of isoniazid action by M tuberculosis KatG
    RICHARD MAGLIOZZO; Fiscal Year: 2009
    ..of both INH and adenine dinucleotides as substrates will be investigated using optical-stopped flow spectrophotometry and rapid freeze-quench electron paramagnetic resonance (EPR)...
  39. QUANTITATIVE TRACE ELEMENT ANALYSIS OF MICRO-SAMPLES
    ROGER BLEILER; Fiscal Year: 1990
    ..These techniques include atomic absorption spectrophotometry (AAS), inductively coupled plasma (ICP), atomic emission spectroscopy (AES) or mass spectrometry (MS)...
  40. MECHANISM OF CARBON TETRACHLORIDE HEPATOTOXICITY
    Jose Castro; Fiscal Year: 1993
    ..goals we plan to perform the necessary studies evidencing that treatments reached liver tissue (GLC; HPLC; spectrophotometry) and/or performed their expected effects (eg...
  41. LONG-RANGE ELECTRON TRANSFERS IN FLAVOCYTOCHROME B2
    CHRISTOPHER KAY; Fiscal Year: 1992
    ..Intermolecular electron transfer to cytochrome c will be studied using stopped-flow spectrophotometry. Steady-state kinetic analysis will identify changes in substrate and produce binding and in catalytic ..
  42. CATALYTIC MECHANISM OF HUMAN MN SUPEROXIDE DISMUTASE
    David Silverman; Fiscal Year: 2004
    ..Pulse radiolysis and scanning stopped-flow spectrophotometry will be used to evaluate changes in catalysis and x-ray crystallography to direct structural changes...
  43. ENZYMATIC DETERMINATION OF FECAL BILE ACIDS
    WILLIAM BEHER; Fiscal Year: 1980
    ..acids are partially destroyed during alkaline hydrolysis, and c) acidic fecal pigments interfere with spectrophotometry at 340 nm. We are developing a new method which will overcome these difficulties...
  44. Flavohemoglobin-catalyzed nitric oxide dioxygenation
    Paul Gardner; Fiscal Year: 2005
    ..2) Intermediates of the NOD enzyme mechanism are being elucidated using stopped flow, EPR, and infrared spectrophotometry, specific traps, and indicators...
  45. TRANSPORT-REGULATED CALCIUM METABOLISM IN TUMOR CELLS
    Gary Fiskum; Fiscal Year: 1990
    ..fluorescent Ca2+ indicators, O2 and Ca2+ electrode measurements, dual beam and dual wavelength spectrophotometry, steady-state measurements of 14CO2 ratios using metabolites labeled at different carbons, and TLC-flame ..
  46. LIPID METABOLISM
    Herbert Kayden; Fiscal Year: 1980
    ..small quantities of tocopherol (less than 1 ng) uses high performance liquid chromatography and fluorescence spectrophotometry. Levels in premature infants suffering from hemolytic anemia or respiratory distress syndrome are easily ..
  47. Gene-Gene Interactions in Pulmonary Hypertension
    ELISABETH WILLERS; Fiscal Year: 2006
    ..include the extraction, purification, and standardization of DNA from blood of patients with PAH, utilizing spectrophotometry, gel electrophoresis and real-time PCR...
  48. Ultrafine Particles and Mitochondrial Dysfunction
    ANNE JOHANSEN; Fiscal Year: 2005
    ..using x-ray photoelectron spectroscopy (XPS), time of flight secondary ion mass spectrometry (TOF-SIMS), and spectrophotometry. Simultaneously, their toxicological effects will be tested in vitro on beef heart mitochondria, with ..
  49. IN VITRO METHODS FOR ISLET ISOLATION AND TRANSPLANTATION
    ROBERT SORENSON; Fiscal Year: 1991
    ..Techniques of immunoassay, morphometric analysis at the light microscopical level, immunocytochemistry, spectrophotometry, chromatography, enzymology, tissue culture, transplantation permit the correlation of morphological, ..
  50. Rheb, mTOR, and Tumorigenesis
    Jessie Hanrahan; Fiscal Year: 2005
    ..effectors will be identified using a 2urn suppressor screen in yeast, 2) Rheb pull-downs coupled with mass spectrophotometry will be utilized to identify associated proteins in both yeast and mammalian cells and 3) the Rheb ..
  51. THERMAL PERTURBATION SPECTROSCOPY OF PEPTIDES
    JAKE BELLO; Fiscal Year: 1980
    Thermal perturbation spectrophotometry will be done on peptide hormones and antibiotics, and on model compounds, in order to obtain information on the environments of aromatic chromophores in peptides...
  52. G-QUADRUPLEX INTERACTIVE COMPOUNDS AS ANTICANCER AGENTS
    Miguel Salazar; Fiscal Year: 2001
    ..using a variety of approaches involving synthetic chemistry, molecular modeling, molecular biology, UV-vis spectrophotometry and other methodologies consistent with the proposed research career plans of the candidate...
  53. MECHANISMS OF PYRIDOXAL-5'-PHOSPHATE DEPENDENT ENZYMES
    Robert Phillips; Fiscal Year: 2004
    ..of the reaction of these enzymes by various kinetic techniques, including rapid scanning stopped flow spectrophotometry and rapid chemical quench...
  54. Functional active-site models of cytochrome c oxidase
    JAMES COLLMAN; Fiscal Year: 2009
    ..The role that the copper ion (CuB) and a phenolic group, in the amino acid tyrosine (Tyr244) play in the catalytic function of CcO are of particular interest. ..
  55. Neuro-Immune Interactions in Developing Airways
    Gary Larsen; Fiscal Year: 2005
    ..These studies of developmental airway biology in mammalian species allow definition of neuro-immune mechanisms that may have relevance to events that occur in infants and small children. ..
  56. Biomimetic Studies of NO-binding Respiratory Chain Hemes
    JAMES COLLMAN; Fiscal Year: 2008
    ..An emphasis will be placed on kinetic and mechanistic studies to elucidate the putitive reaction pathways of CcO and NOR. ..
  57. NEUROENDOCRINE ABNORMALITIES INDUCED BY SLEEP DEPRIVATIO
    CAROL EVERSON; Fiscal Year: 2001
    ..These studies will delineate the abnormalities in brain endocrine regulation resulting from sleep deprivation and determine the mechanisms responsible for peripheral endocrine changes that underlie its associated systemic pathologies. ..
  58. Mechanisms of Herbal Radiosensitization of Glioma Cells
    Garret Yount; Fiscal Year: 2002
    ..abstract_text> ..
  59. Heme Acquisition in Shigella Dysenteriae.
    Angela Wilks; Fiscal Year: 2005
    ..together with physical techniques such as optical absorption, fluorescence, MCD and resonance Raman spectrophotometry will provide information on the structural features required for heme-binding and transport...
  60. Oral Cancer, Chemoprevention, and Anti-Angiogenesis
    Mark Lingen; Fiscal Year: 2009
    ..abstract_text> ..
  61. Metabolism of Carcinogens and Drugs by Human P450s
    F Guengerich; Fiscal Year: 2005
    ..Successful completion of these collective studies should provide better understanding of the ways in which human P450s catalyze oxidation of carcinogens and drugs and the significance of variations in these enzymes. ..