fluorometry

Summary

Top Publications

  1. ncbi Calibration of the calcium dissociation constant of Rhod(2)in the perfused mouse heart using manganese quenching
    C Du
    NSF Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA, USA
    Cell Calcium 29:217-27. 2001
  2. ncbi A fluorometric assay for detection of lysyl oxidase enzyme activity in biological samples
    Amitha H Palamakumbura
    Division of Oral Biology, Boston University Goldman School of Dental Medicine, 100 East Newton Street, Boston, Massachusetts 02118, USA
    Anal Biochem 300:245-51. 2002
  3. ncbi The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability
    Frank H Niesen
    Structural Genomics Consortium, Botnar Research Centre, Oxford University, Oxford, UK
    Nat Protoc 2:2212-21. 2007
  4. pmc Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation
    D Campbell
    Department of Biology, Mount Allison University, Sackville, New Brunswick E4L 1G7, Canada
    Microbiol Mol Biol Rev 62:667-83. 1998
  5. pmc A biochemical fluorometric method for assessing the oxidative properties of HDL
    Theodoros Kelesidis
    Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    J Lipid Res 52:2341-51. 2011
  6. pmc State-dependent cAMP binding to functioning HCN channels studied by patch-clamp fluorometry
    Shengjun Wu
    Department of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, Virginia, USA
    Biophys J 100:1226-32. 2011
  7. pmc Optimization of variable fluorescence measurements of phytoplankton communities with cyanobacteria
    Stefan G H Simis
    Finnish Environment Institute SYKE, Marine Research Centre, Erik Palménin aukio 1, 00560 Helsinki, Finland
    Photosynth Res 112:13-30. 2012
  8. ncbi Interdependence of receptor activation and ligand binding in HCN2 pacemaker channels
    Jana Kusch
    Institut fur Physiologie II, Universitatsklinikum Jena, Jena, Germany
    Neuron 67:75-85. 2010
  9. ncbi A rapid direct fluorescent assay for cell-free DNA quantification in biological fluids
    Hagit Goldshtein
    Department of Clinical Biochemistry, Ben Gurion University of the Negev, Israel
    Ann Clin Biochem 46:488-94. 2009
  10. ncbi Fitting light saturation curves measured using modulated fluorometry
    Raymond J Ritchie
    School of Biological Sciences A 08, The University of Sydney, Sydney, NSW 2006, Australia
    Photosynth Res 96:201-15. 2008

Detail Information

Publications240 found, 100 shown here

  1. ncbi Calibration of the calcium dissociation constant of Rhod(2)in the perfused mouse heart using manganese quenching
    C Du
    NSF Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA, USA
    Cell Calcium 29:217-27. 2001
    ..This is in good agreement with values obtained by calibration of fluorescence values with a calcium saturation tetanization procedure in the same perfused mouse heart model...
  2. ncbi A fluorometric assay for detection of lysyl oxidase enzyme activity in biological samples
    Amitha H Palamakumbura
    Division of Oral Biology, Boston University Goldman School of Dental Medicine, 100 East Newton Street, Boston, Massachusetts 02118, USA
    Anal Biochem 300:245-51. 2002
    ..This method eliminates the interference that occurs in some biological samples and can be successfully used to detect lysyl oxidase activity in cell culture experiments...
  3. ncbi The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability
    Frank H Niesen
    Structural Genomics Consortium, Botnar Research Centre, Oxford University, Oxford, UK
    Nat Protoc 2:2212-21. 2007
    ..Results can be obtained in a single day...
  4. pmc Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation
    D Campbell
    Department of Biology, Mount Allison University, Sackville, New Brunswick E4L 1G7, Canada
    Microbiol Mol Biol Rev 62:667-83. 1998
    ..Finally, fluorescence parameters can be used to estimate the electron transport rate at the acclimated growth light intensity...
  5. pmc A biochemical fluorometric method for assessing the oxidative properties of HDL
    Theodoros Kelesidis
    Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    J Lipid Res 52:2341-51. 2011
    ..This new fluorometric method offers an inexpensive, accurate, and rapid means for determining the oxidative properties of HDL that is applicable to large-scale clinical studies...
  6. pmc State-dependent cAMP binding to functioning HCN channels studied by patch-clamp fluorometry
    Shengjun Wu
    Department of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, Virginia, USA
    Biophys J 100:1226-32. 2011
    ..HCN) channel by simultaneously recording channel opening and ligand binding, using the patch-clamp fluorometry technique with a unique fluorescent cAMP analog that fluoresces strongly in the hydrophobic binding pocket and ..
  7. pmc Optimization of variable fluorescence measurements of phytoplankton communities with cyanobacteria
    Stefan G H Simis
    Finnish Environment Institute SYKE, Marine Research Centre, Erik Palménin aukio 1, 00560 Helsinki, Finland
    Photosynth Res 112:13-30. 2012
    ..65-0.7...
  8. ncbi Interdependence of receptor activation and ligand binding in HCN2 pacemaker channels
    Jana Kusch
    Institut fur Physiologie II, Universitatsklinikum Jena, Jena, Germany
    Neuron 67:75-85. 2010
    ..Using patch-clamp fluorometry and a fluorescent cAMP analog, we show that full ligand-induced activation appears already with only two ..
  9. ncbi A rapid direct fluorescent assay for cell-free DNA quantification in biological fluids
    Hagit Goldshtein
    Department of Clinical Biochemistry, Ben Gurion University of the Negev, Israel
    Ann Clin Biochem 46:488-94. 2009
    ..Owing to the complex and expensive methodology, detection of CFD has hitherto been confined to research laboratories. This study presents a simple, inexpensive and accurate test for CFD...
  10. ncbi Fitting light saturation curves measured using modulated fluorometry
    Raymond J Ritchie
    School of Biological Sciences A 08, The University of Sydney, Sydney, NSW 2006, Australia
    Photosynth Res 96:201-15. 2008
    ..The kinetics of NPQ does not appear to be related to the kinetics of Phi(PSII) or rETR...
  11. ncbi Simultaneous multiple target detection in real-time loop-mediated isothermal amplification
    Nathan A Tanner
    New England Biolabs, Ipswich, MA, USA
    Biotechniques 53:81-9. 2012
    ..The coupling of this new multiplex technique with next generation isothermal DNA polymerases should increase the utility of the LAMP method for molecular diagnostics...
  12. ncbi Confounding experimental considerations in nanogenotoxicology
    S H Doak
    Institute of Life Science, Swansea University, Wales, UK
    Mutagenesis 24:285-93. 2009
    ..Thus, highlighting some of the potential confounding factors that need to be considered in order to ensure that in vitro genotoxicity assays report true biological impacts in response to nanomaterial exposure...
  13. pmc Dynamics of Ca2+-calmodulin-dependent inhibition of rod cyclic nucleotide-gated channels measured by patch-clamp fluorometry
    Matthew C Trudeau
    Dept of Physiology and Biophysics, Box 357290, University of Washington, Seattle, WA 98195 7290, USA
    J Gen Physiol 124:211-23. 2004
    ..This motion occurs with the same time course as channel inhibition, consistent with the notion that rearrangement of the NH(2)- and COOH-terminal regions underlies Ca(2+)/CaM-dependent inhibition...
  14. ncbi Seasonal and diel changes in photosynthetic activity of the snow alga Chlamydomonas nivalis (Chlorophyceae) from Svalbard determined by pulse amplitude modulation fluorometry
    Marek Stibal
    Faculty of Biological Sciences, University of South Bohemia, Ceske Budejovice, Czechia
    FEMS Microbiol Ecol 59:265-73. 2007
    ..Photosynthetic activity was measured using pulse amplitude modulation fluorometry. Three types of cell (green biflagellate vegetative cells, orange spores clustered by means of mucilaginous ..
  15. pmc Interaction of Escherichia coli RNA polymerase σ70 subunit with promoter elements in the context of free σ70, RNA polymerase holoenzyme, and the β'-σ70 complex
    Vladimir Mekler
    Waksman Institute of Microbiology, Rutgers, State University of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 286:270-9. 2011
    ..The new fluorescent assay, which we call a protein beacon assay, will be instrumental in quantitative dissection of fine details of RNAP interactions with promoters...
  16. ncbi Interaction of beta-lactoglobulin with small hydrophobic ligands as monitored by fluorometry and equilibrium dialysis: nonlinear quenching effects related to protein--protein association
    S Muresan
    Agrotechnological Research Institute ATO, Wageningen University and Research Center, P.O. Box 17, 6700 AA Wageningen, The Netherlands
    J Agric Food Chem 49:2609-18. 2001
    ..The bindings of several small ligands as detected by fluorometry and equilibrium dialysis were compared...
  17. pmc Differential scanning fluorometry signatures as indicators of enzyme inhibitor mode of action: case study of glutathione S-transferase
    Wendy A Lea
    NIH Chemical Genomics Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA
    PLoS ONE 7:e36219. 2012
    Differential scanning fluorometry (DSF), also referred to as fluorescence thermal shift, is emerging as a convenient method to evaluate the stabilizing effect of small molecules on proteins of interest...
  18. ncbi Modeling and design of micromachined optical Söller collimators for lensless CCD-based fluorometry
    Joshua Balsam
    University of Maryland College Park UMCP, College Park, MD 20742, USA
    Analyst 137:5011-7. 2012
    ..In this paper, we describe the optical principle for designing these Söller collimators for lensfree CCD-based fluorometry. The illuminating surface was modeled as a collection of differential areas emitting uniformly and spherically, ..
  19. ncbi PicoGmeter, a custom-made fluorometer for the quantification of dsDNA by PicoGreen fluorescence
    Geoffrey G Schofield
    Department of Physiology, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Biotechniques 37:778-80, 782. 2004
    ..This simple, inexpensive, battery-operated fluorometer will allow investigators to employ the PicoGreen method without incurring the cost of purchasing a spectrofluorometer...
  20. ncbi Autofluorescence method to measure macular pigment optical densities fluorometry and autofluorescence imaging
    Francois C Delori
    Schepens Eye Research Institute, Boston, MA, USA
    Arch Biochem Biophys 430:156-62. 2004
    ..The method is implemented either by fluorometry of lipofuscin to yield the optical density of the macular pigment in a 2 degrees diameter central area, or by ..
  21. pmc Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid
    V Hukkanen
    Department of Virology, University of Turku, FIN 20520 Turku, Finland
    J Clin Microbiol 38:3214-8. 2000
    We have introduced a time-resolved fluorometry (TRF)-based microwell hybridization assay for PCR products in detection of herpes simplex virus (HSV) in cerebrospinal fluid (CSF) specimens...
  22. ncbi An immunoassay for the pathological form of the prion protein based on denaturation and time resolved fluorometry
    Reza H Dabaghian
    Virus Reference Department, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK
    J Virol Methods 132:85-91. 2006
    ..The assay has a detection limit of 10 pg PrP, is robust and could be automated...
  23. ncbi Comparison by PAM fluorometry of photosynthetic activity of nine marine phytoplankton grown under identical conditions
    P Juneau
    Department of Earth and Ocean Sciences, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
    Photochem Photobiol 81:649-53. 2005
    ....
  24. ncbi PAM fluorometry as a tool to assess microalgal nutrient stress and monitor cellular neutral lipids
    S White
    Institute of Water and Wastewater Research, Durban University of Technology, P O Box 1334, Durban 4000, South Africa
    Bioresour Technol 102:1675-82. 2011
    This study investigated the use of Pulse Amplitude Modulated (PAM) fluorometry to measure nutrient induced physiological stress and subsequent synthesis of cellular neutral lipids. A freshwater Chlorella sp...
  25. ncbi Study on the subgel-phase formation using an asymmetric phospholipid bilayer membrane by high-pressure fluorometry
    Masaki Goto
    Department of Life System, Institute of Technology and Science, The University of Tokushima, Tokushima, Japan
    Langmuir 28:12191-8. 2012
    ..Taking into account the fluorescence behavior of Prodan for the L(c) phase, we concluded that the structure of the L(c) phase is highly probably a staggered structure, which can transform into the L(c)I phase easily...
  26. ncbi A coupled equilibrium approach to study nucleosome thermodynamics
    Andrew J Andrews
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado, USA
    Methods Enzymol 488:265-85. 2011
    ..This method will be useful for examining the impact of DNA sequence, histone variants, and posttranslational modifications on nucleosome thermodynamics...
  27. pmc Fluorometric measurements of conformational changes in glutamate transporters
    H Peter Larsson
    Neurological Sciences Institute, Oregon Health and Science University, 505 Northwest 185th Avenue, Beaverton, OR 97006, USA
    Proc Natl Acad Sci U S A 101:3951-6. 2004
    ..In the present study, we used voltage-clamp fluorometry to measure conformational changes in the neuronal excitatory amino acid transporter (EAAT) 3 glutamate ..
  28. ncbi Intra-leaf gradients of photoinhibition induced by different color lights: implications for the dual mechanisms of photoinhibition and for the application of conventional chlorophyll fluorometers
    Riichi Oguchi
    Plant Sciences, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan
    New Phytol 191:146-59. 2011
    ....
  29. ncbi Analysis of intracellular oxygen and metabolic responses of mammalian cells by time-resolved fluorometry
    Alexander V Zhdanov
    Luxcel Biosciences Ltd, BioInnovation Centre, UCC, Cork, Ireland
    Anal Chem 79:9414-9. 2007
    ..The assay provides relevant, information-rich data on cellular function and metabolism. It allows monitoring of small, rapid, and transient changes in cell respiration and screening of new chemical entities...
  30. ncbi Green light drives leaf photosynthesis more efficiently than red light in strong white light: revisiting the enigmatic question of why leaves are green
    Ichiro Terashima
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Plant Cell Physiol 50:684-97. 2009
    ..We also discuss some serious problems that are caused by neglecting these intra-leaf profiles when estimating whole leaf electron transport rates and assessing photoinhibition by fluorescence techniques...
  31. ncbi Application of fluorescent protein-tagged trans factors and immobilized cis elements to monitoring of toxic metals based on in vitro protein-DNA interactions
    Yasunari Kawakami
    Faculty of Agriculture, Utsunomiya University, 350 Minemachi, Utsunomiya 321 8505, Japan
    Biosens Bioelectron 26:1466-73. 2010
    ..This is the first demonstration of a simple and rapid fluorometry to detect analytes based on in vitro interaction between a cis element and a trans factor.
  32. ncbi Illuminating the structure and function of Cys-loop receptors
    Stephan A Pless
    School of Biomedical Sciences and Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia
    Clin Exp Pharmacol Physiol 35:1137-42. 2008
    ..Voltage-clamp fluorometry helps overcome this problem by simultaneously monitoring movements at the channel gate (through changes in ..
  33. pmc Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluorometry reveals complex action of isoflurane on cardiomyocytes
    Filip Sedlic
    Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochim Biophys Acta 1797:1749-58. 2010
    ..The NAD(P)H-FP fluorometry is a useful method for exploring the effect of drugs on mitochondria and identifying their specific sites of ..
  34. ncbi Investigation of the micellar properties of the tocopheryl polyethylene glycol succinate surfactants TPGS 400 and TPGS 1000 by steady state fluorometry
    M Sadoqi
    Physics Department, St John s University, 8000, Utopia Parkway, Jamaica, NY 11439, USA
    J Colloid Interface Sci 333:585-9. 2009
    ....
  35. ncbi Simultaneous determination of carvedilol and ampicillin sodium by first-derivative fluorometry in the presence of human serum albumin
    Huai You Wang
    College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, People s Republic of China
    Anal Sci 21:537-40. 2005
    ..The recoveries of carvedilol and AS were measured. This method is simple and can be used for the determination of carvedilol and AS in human serum and urine samples with satisfactory results...
  36. ncbi Diagnosis of enterovirus and rhinovirus infections by RT-PCR and time-resolved fluorometry with lanthanide chelate labeled probes
    M Lonnrot
    Department of Virology, Medical School and University Hospital of Tampere University, Tampere, Finland
    J Med Virol 59:378-84. 1999
    ..probes carrying lanthanide chelate labels, which can be detected simultaneously by time-resolved fluorometry. The sensitivity and specificity of the RT-PCR-hybridization method were evaluated with a representative ..
  37. pmc Voltage-dependent conformational changes in human Ca(2+)- and voltage-activated K(+) channel, revealed by voltage-clamp fluorometry
    Nicoletta Savalli
    Departments of Anesthesiology Division of Molecular Medicine, Brain Research Institute, David Geffen School of Medicine at University of California, Los Angeles, CA 90095 7115, USA
    Proc Natl Acad Sci U S A 103:12619-24. 2006
    ..By site-directed fluorescent labeling of residues in the S3-S4 region and by using voltage clamp fluorometry, we have resolved the conformational changes the channel undergoes during activation...
  38. ncbi Results from the "Technical workshop on genotoxicity biosensing" on the micro-scale fluorometric assay of deoxyribonucleic acid unwinding
    Christa Baumstark-Khan
    Radiation Biology, Institute for Aerospace Medicine, German Aerospace Research Center DLR Köln, Linder Hohe, 51147 Köln, Germany
    Anal Chim Acta 593:75-81. 2007
    ..As an inter-assay standard the action of hydrogen peroxide was tested in every test plate. The results demonstrate that the micro-FADU assay is suitable to detect the presence of chemically induced strand breaks within 3 h...
  39. ncbi Higher serum prolyl endopeptidase activity in patients with post-traumatic stress disorder
    M Maes
    Clinical Research Center for Mental Health, Antwerp, Belgium
    J Affect Disord 53:27-34. 1999
    ..4.21.26), a cytosolic endopeptidase, which cleaves peptide bonds on the carboxylside of proline in proteins of relatively small molecular mass...
  40. pmc Time-resolved fluorometry based sandwich hybridisation assay for HLA-DQA1 typing
    M Sjöroos
    Department of Biotechnology, University of Turku, Finland
    Dis Markers 14:9-19. 1998
    ..A series of whole blood spot samples were also studied and the results confirmed the applicability of this modification of the test...
  41. pmc A direct demonstration of closed-state inactivation of K+ channels at low pH
    Thomas W Claydon
    Department of Anesthesiology, Pharmacology, and Therapeutics, University of British Columbia, Vancouver, BC, Canada
    J Gen Physiol 129:437-55. 2007
    ....
  42. ncbi Simultaneous direct determination of amiloride and triamterene in urine using isopotential fluorometry
    J A Pulgarín
    Department of Analytical Chemistry and Food Technology, University of Castilla La Mancha, Ciudad Real, E 13071, Spain
    Anal Biochem 292:59-68. 2001
    ..The limit of detection calculated according to Clayton who uses error propagation throughout the calibration curve and a noncentralized security factor was 16.8 and 2.4 ng mL(-1) for amiloride and triamterene, respectively...
  43. ncbi Cumulative glycaemia as measured by lens fluorometry: association with retinopathy in type 2 diabetes
    I C Munch
    Department of Ophthalmology, Glostrup Hospital, University of Copenhagen, Nordre Ringvej 59, 2600 Glostrup, Denmark
    Diabetologia 54:757-61. 2011
    The aim of this study was to assess the association between lifelong cumulative glycaemia estimated by lens fluorometry and the presence of retinopathy in individuals with type 2 diabetes.
  44. ncbi Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry
    T Nishigaki
    , , Cuernavaca, Morelos, 62250,
    Biochem Biophys Res Commun 284:531-5. 2001
    Speract, a decapeptide from sea urchin egg jelly, induces various sperm responses. Stopped-flow fluorometry was used to examine the binding of labeled speract and the intracellular changes in pH (pH(i)) and Ca2+ ([Ca2+]i) it induces in ..
  45. ncbi The voltage-clamp fluorometry technique
    Chris S Gandhi
    Division of Chemistry and Chemical Engineering, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA, USA
    Methods Mol Biol 491:213-31. 2008
    ..Here we describe the optical technique voltage-clamp fluorometry (VCF) which is used to monitor the functional state and probe the structural rearrangements that take place as ..
  46. ncbi Comparison of Cd, Cu, and Zn toxic effects on four marine phytoplankton by pulse-amplitude-modulated fluorometry
    Ai Jun Miao
    Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Environ Toxicol Chem 24:2603-11. 2005
    ..For all the algae examined in our study, the bioaccumulation potentials of Cu and Zn were similar to each other, while the Cd bioaccumulation was much lower under environmentally realistic metal concentration...
  47. ncbi Estimation of chlorophyll content and daily primary production of the major algal groups by means of multiwavelength-excitation PAM chlorophyll fluorometry: performance and methodological limits
    Torsten Jakob
    Biology I Plant Physiology, University of Leipzig, Germany
    Photosynth Res 83:343-61. 2005
    ..While this aspect presently limits the application of PAM fluorometry in limnology, this limitation may be overcome by future technical progress in the detection of dynamic changes ..
  48. ncbi Hydrophilic labeling reagents of dipyrrylmethene-BF2 dyes for two-photon excited fluorometry: syntheses and photophysical characterization
    Niko J Meltola
    Laboratory of Biophysics, Institute of Biomedicine, University of Turku, PO Box 123, 20521 Turku, Finland
    J Fluoresc 14:635-47. 2004
    ..The results suggest that the novel dipyrrylmethene-BF2 labels are highly applicable to bioaffinity assays based on two-photon excitation of fluorescence...
  49. ncbi Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry
    Y Itoh
    Research Laboratories, Nippon Shinyaku Co, Ltd, Nishiohji Hachijo Minami ku, Kyoto, 601, Japan
    Anal Biochem 287:203-9. 2000
    ..Therefore, the present novel diaminofluorescein fluorometry should be useful not only for sensitive NO assay, but also for NO imaging in a variety of biological specimens.
  50. ncbi A continuous fluorescent assay for the determination of plasma and tissue angiotensin I-converting enzyme activity
    M F Alves
    Departamento de Biofisica, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brasil
    Braz J Med Biol Res 38:861-8. 2005
    ..5 microM lisinopril or captopril. The advantages of the method are the procedural simplicity and the high sensitivity providing a rapid assay for ACE determinations...
  51. pmc Conformational dynamics of the Na+/K+-ATPase probed by voltage clamp fluorometry
    Sven Geibel
    Max Planck Institute of Biophysics, Kennedyallee 70, D 60596 Frankfurt, Germany
    Proc Natl Acad Sci U S A 100:964-9. 2003
    The method of voltage clamp fluorometry combined with site-directed fluorescence labeling was used to detect local protein motions of the fully active Na(+)K(+)-ATPase in real time under physiological conditions...
  52. ncbi High-throughput genetic analysis using time-resolved fluorometry and closed-tube detection
    J Nurmi
    Department of Biotechnology, University of Turku, Tykistokatu 6A, 6th Floor, 20520 Turku, Finland
    Anal Biochem 299:211-7. 2001
    ..Throughput can be maximized by using predried PCR mixtures that are stable for at least 6 months. This homogenous, all-in-one dry reagent assay chemistry permits cost-effective genetic screening on a large scale...
  53. ncbi Fluorometric analysis of DNA unwinding (FADU) as a method for detecting repair-induced DNA strand breaks in UV-irradiated mammalian cells
    C Baumstark-Khan
    Project Group Human Radiation Risk, Institute of Aerospace Medicine, Medical Faculty of the RWTH Aachen, Germany
    Photochem Photobiol 72:477-84. 2000
    ....
  54. pmc Ligand-dependent effects on the conformational equilibrium of the Na+,K+-ATPase as monitored by voltage clamp fluorometry
    Stefan A Geys
    Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
    Biophys J 96:4561-70. 2009
    Voltage clamp fluorometry was used to monitor conformational changes associated with electrogenic partial reactions of the Na(+),K(+)-ATPase after changes in the concentration of internal sodium (Na(+)(i)) or external potassium (K(+)(o))...
  55. pmc New separation-free assay technique for SNPs using two-photon excitation fluorometry
    Jonne Vaarno
    Laboratory of Biophysics, Institute of Biomedicine, University of Turku, Finland
    Nucleic Acids Res 32:e108. 2004
    ..Applicability of the new method in routine laboratory use is discussed with respect to alternative assay techniques...
  56. pmc An automated microplate-based method for monitoring DNA strand breaks in plasmids and bacterial artificial chromosomes
    Cassandra Rock
    The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK
    Nucleic Acids Res 31:e65. 2003
    ..Results obtained from pulsed- field electrophoresis of sheared samples were in agreement with those obtained using the microplate-based method...
  57. ncbi Two-photon excitation fluorometric measurement of homogeneous microparticle immunoassay for C-reactive protein
    Matti E Waris
    Laboratory of Biophysics, Institute of Biomedicine, Turku University Central Hospital, University of Turku, P O Box 123, FIN 20521, 20520 Turku, Finland
    Anal Biochem 309:67-74. 2002
    ..laser technology have enabled the design of a compact instrumentation for two-photon excitation microparticle fluorometry (TPX)...
  58. ncbi Derivatization of F2-isoprostanes with 1-pyrenyldiazomethane and their subsequent determination by fluorescence high-performance liquid chromatography
    Vladimir B Ritov
    Department of Medicine, School of Medicine, Division of Endocrinology and Metabolism, University of Pittsburgh, 3459 Fifth Avenue, MUH N809, Pittsburgh, PA 15213 3236, USA
    Anal Biochem 311:10-8. 2002
    ..The purified 1-pyrenylmethyl esters of PGF(2alpha) were quantitatively analyzed by reverse-phase HPLC with fluorescent detection, with a detection limit of 5-10 pg...
  59. ncbi Sulfonylureas rapidly cross phospholipid bilayer membranes by a free-diffusion mechanism
    Frits Kamp
    Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts, USA
    Diabetes 52:2526-31. 2003
    ..Thus, we now provide a molecular mechanism by which sulfonylureas can penetrate the plasma membrane and reach intracellular sites regulating exocytosis...
  60. ncbi A microplate fluorimetric assay for measuring dehalogenase activity
    Julian R Marchesi
    Department of Microbiology, University College Cork, Cork, Ireland
    J Microbiol Methods 55:325-9. 2003
    ..The assay shows a wide response to halide concentration (1-500 mM) and tolerates a wide pH range. Furthermore, it is simple to use, has the potential for automation and uses an inexpensive non-toxic reagent...
  61. ncbi Selective zinc sensor molecules with various affinities for Zn2+, revealing dynamics and regional distribution of synaptically released Zn2+ in hippocampal slices
    Kensuke Komatsu
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 127:10197-204. 2005
    ..We believe that the use of various combinations of ZnAF family members will offer unprecedented versatility for fluorescence-microscopic imaging of Zn(2+) in biological applications...
  62. pmc A real-time DNase assay (ReDA) based on PicoGreen fluorescence
    Gökhan Tolun
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, PO Box 016129, Miami, FL 33101 6129, USA
    Nucleic Acids Res 31:e111. 2003
    ..The benefits of, and limitations to, the method are discussed...
  63. ncbi A rapid and sensitive fluorometric assay method for the determination of nitrilase activity
    Anirban Banerjee
    Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Sector 67, Mohali, Punjab 160062, India
    Biotechnol Appl Biochem 37:289-93. 2003
    ..The activity determined by the proposed method correlates ( r =0.9625) well with the established Berthelot method. The proposed method is more sensitive than the existing methods for the determination of nitrilase activity...
  64. ncbi Intracellular labeling method for chip-based capillary electrophoresis fluorimetric single cell analysis using liposomes
    Yue Sun
    Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310027, China
    J Chromatogr A 1135:109-14. 2006
    ..Single cell analysis using microfluidic chip-based CE revealed that liposome-membrane fusion occurred after entrance of liposomes into the cells, with release of encapsulated fluorescence dyes and labeling of intracellular species...
  65. ncbi Identification of metabotropic glutamate receptor antagonists using an automated high-throughput screening system
    Peter Hodder
    Department of Automated Biotechnology, Merck Research Laboratories, North Wales, Pennsylvania 19454, USA
    Anal Biochem 313:246-54. 2003
    ..Primary high-throughput screening hits were subjected to a combination of data analysis and counterscreening assays to identify several compounds with both efficacy and selectivity for the metabotropic glutamate receptor target...
  66. ncbi Rapid analysis of amino sugars by microchip electrophoresis with laser-induced fluorescence detection
    S Suzuki
    Faculty of Pharmaceutical Sciences, Kini, University, Higashi-Osaka, Japan
    Electrophoresis 22:4023-31. 2001
    ..The result of amino sugar composition analysis for individual O-glycans fractionated by high-performance liquid chromatography was quite useful for their identification...
  67. ncbi Determination of the activity of catalase using a europium(III)-tetracycline-derived fluorescent substrate
    Meng Wu
    Institute of Analytical Chemistry, Chemo and Biosensors, University of Regensburg, D 93040 Regensburg, Germany
    Anal Biochem 320:129-35. 2003
    ..It is also the first europium-based probe that is compatible with the 405-nm diode laser. In summary, the new assay provides distinct advantages over direct ultraviolet detection and over the two-reagent (peroxidase) method...
  68. ncbi Fluorimetric determination of phytic acid in urine based on replacement reaction
    Yingyu Chen
    School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China
    Anal Chim Acta 605:185-91. 2007
    ..49-0.75 mg L(-1) with recoveries of 96.2-108.8%. Comparison of the obtained results with the reported HPLC was performed, indicating the proposed method was reliable...
  69. ncbi Critical evaluation of cytosolic calcium determination in resting muscle fibres from normal and dystrophic (mdx) mice
    P Gailly
    Department of Physiology, UCL 5540, Brussels, Belgium
    Cell Calcium 14:473-83. 1993
    ..We showed that the behavior of the dye was not similar in C57 and in mdx muscles. For this reason, we did not confirm the previous results that cytosolic free calcium concentration is increased in mdx muscles...
  70. ncbi A ready-to-use fluorimetric biosensor for superoxide radical using superoxide dismutase and peroxidase immobilized in sol-gel glasses
    Isabel Pastor
    Instituto de Biologia Molecular y Celular, Universidad Miguel Hernandez, Elche E 03202, Spain
    Anal Biochem 334:335-43. 2004
    ..These facts make this biosensor a simple, reliable, and highly sensitive method with a potential use in biological systems, food, and drinks...
  71. ncbi Distribution of acridine orange fluorescence in Plasmodium falciparum-infected erythrocytes and its implications for the evaluation of digestive vacuole pH
    Patrick G Bray
    Liverpool School of Tropical Medicine, UK
    Mol Biochem Parasitol 119:301-4; discussion 307-9, 311-3. 2002
  72. ncbi Fluorescent nanoparticles as labels for immunometric assay of C-reactive protein using two-photon excitation assay technology
    Janne O Koskinen
    Laboratory of Biophysics, Institute of Biomedicine, University of Turku, Finland
    Anal Biochem 328:210-8. 2004
    ..The new assay method was applied for assessment of baseline levels of CRP in sera of apparently healthy individuals...
  73. ncbi Detection of residual donor leucocytes in leucoreduced red blood cell components using a fluorescence microplate assay
    Ruth L Gilbert
    Bristol Institute for Transfusion Sciences, National Blood Service Bristol Centre, Southmead Road, Bristol BS10 5ND, UK
    J Immunol Methods 274:17-25. 2003
    ..175). In conclusion, the fluorescence microplate assay represents a simple, high throughput alternative to flow cytometry for monitoring leucodepletion compliance in blood banks...
  74. ncbi A rapid fluorometric assay for the proteolytic activity of SKI-1/S1P based on the surface glycoprotein of the hemorrhagic fever Lassa virus
    Ajoy Basak
    Regional Protein Chemistry Center, Diseases of Ageing Unit, Ottawa Health Research Institute, Loeb Building, 725 Parkdale Ave, Ottawa, Ontario, Canada K1Y 4E9
    FEBS Lett 514:333-9. 2002
    ..This study confirmed the role of SKI-1 in GP-C processing and provides a novel, rapid and efficient enzymatic assay of SKI-1...
  75. ncbi High-performance liquid chromatography determination of methionine adenosyltransferase activity using catechol-O-methyltransferase-coupled fluorometric detection
    Sheng Hung Wang
    Graduate Institute of Pharmaceutical Chemistry, China Medical College, Taichung, Taiwan, ROC
    Anal Biochem 319:13-20. 2003
    ..The kinetic study showed that MAT in HL-60 cells exhibited negative cooperativity with a Hill coefficient of 0.5. The values of K(m) and V(max) were 6.1+/-0.3 microM and 135.4+/-1.5 nmol AdoMet formed/mg protein/h, respectively...
  76. ncbi Simultaneous determination of D- and L-serine in rat brain microdialysis sample using a column-switching HPLC with fluorimetric detection
    Takeshi Fukushima
    Department of Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
    Biomed Chromatogr 18:813-9. 2004
    ..The proposed HPLC method will be useful for in vivo studies on D-serine, which acts as a coagonist for N-methyl-D-aspartate receptor, to the extracellular fluid of rat brain...
  77. ncbi Measurement of DNA single-strand breaks by alkaline elution and fluorometric DNA quantification
    Marina Goumenou
    Laboratory of Pesticides Toxicology, Benaki Phytopathological Institute, Kifissia, GR 145 61 Athens, Greece
    Anal Biochem 326:146-52. 2004
    ..The method of the DNA alkaline elution followed by fluorometric determination of ssDNA as modified and evaluated is an accurate and reliable approach for the determination of in vivo induced ssDNA strand breaks...
  78. ncbi High performance liquid chromatography for quantification of gatifloxacin in rat plasma following automated on-line solid phase extraction
    Leandro Tasso
    Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de Farmacia, Universidade Federal do Rio Grande do Sul, Av Ipiranga, 2752, Porto Alegre, RS 90 610 000, Brazil
    J Pharm Biomed Anal 44:205-10. 2007
    ..The assay has been successfully applied to measure gatifloxacin plasma concentrations in pharmacokinetic study in rats...
  79. ncbi Optimisation of an enzymatic method for beta-galactosidase
    James B J McGuire
    Department of Clinical Biochemistry, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
    Clin Chim Acta 326:123-9. 2002
    ..The enzyme beta-galactosidase present in the Kupffer cells of the liver has potential as a marker of liver dysfunction prior to transplantation. Spectrophotometric methods have insufficient sensitivity...
  80. ncbi Single-cell and subcellular measurement of intracellular Ca2+ concentration
    Anthony J Morgan
    Physiology Group, Vascular Biology Research Centre, Biomedical Sciences Division, King s College London, UK
    Methods Mol Biol 312:87-117. 2006
  81. ncbi Ratiometric, fluorescent BODIPY dye with aza crown ether functionality: synthesis, solvatochromism, and metal ion complex formation
    Wenwu Qin
    Department of Chemistry and Institute for Nanoscale Physics and Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200f bus 02404, 3001 Leuven, Belgium
    J Phys Chem A 112:6104-14. 2008
    ..The compound also forms 1:1 complexes with several metal ions (Li(+), Na(+), Mg(2+), Ca(2+), Ba(2+), Zn(2+)), producing large blue shifts in the excitation spectra and significant cation-induced fluorescence amplifications...
  82. ncbi No-wash dyes for calcium flux measurement
    Christopher Mehlin
    Molecumetics Ltd, Bellevue, WA, USA
    Biotechniques 34:164-6. 2003
  83. ncbi Evaluation of FLIPR Calcium 3 Assay Kit--a new no-wash fluorescence calcium indicator reagent
    Yingxin Zhang
    Neuroscience Discovery Research, Wyeth Research, Princeton, NJ 08543, USA
    J Biomol Screen 8:571-7. 2003
    ..Our results validate Calcium 3 as a compelling alternative to FLUO-4 in the choice of fluorescent dye reagent for studying G-protein-coupled receptors, providing the advantage of a homogenous, no-wash assay format...
  84. ncbi Quantification of fluorophore concentration in vivo using two simple fluorescence-based measurement techniques
    Kevin R Diamond
    Juravinski Cancer Centre and McMaster University, Department of Medical Physics, 699 Concession Street, Hamilton, Ontario, Canada L8V 5C2
    J Biomed Opt 10:024007. 2005
    ..In liver and kidney an additional short lifetime component was evident...
  85. ncbi Self-referencing intensity measurements based on square-wave gated phase-modulation fluorimetry
    H M Rowe
    Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904 4319, USA
    Appl Spectrosc 57:532-7. 2003
    ..A key element of the adapted GPM method is its insensitivity to fluctuations in the source intensity. For a roughly 8-fold change in the excitation intensity, the signal ratio changes by less than 3%...
  86. ncbi A continuous fluorimetric assay for tumor necrosis factor-alpha converting enzyme
    Guixian Jin
    Department of Biological Chemistry, Wyeth Ayerst Research, 401 North Middletown Road, Pearl River, New York 10965, USA
    Anal Biochem 302:269-75. 2002
    ..TACE appears to slightly prefer the A-V scissile bond. The enzyme also cleaves scissile bonds such as F-V, A-I, and A-L efficiently...
  87. ncbi A europium chelate for quantitative point-of-care immunoassays using direct surface measurement
    Piia von Lode
    Department of Biotechnology, University of Turku, Tykistokatu 6A, 6th Floor, FIN 20520 Turku, Finland
    Anal Chem 75:3193-201. 2003
    ..The novel nonadentate label facilitates short turnaround times and simple instrumentation due to the absence of all signal development steps, at the same time retaining an excellent immunoassay performance...
  88. ncbi Comparison of LST + MUG broth technique and conventional method for the enumeration of Escherichia coli in foods
    H B Dogan
    Department of Food Engineering, Faculty of Agriculture, Ankara University, Turkey
    Lett Appl Microbiol 34:274-8. 2002
    ..This study provides reliability data for the fluorogenic method applied to certain foods...
  89. ncbi Application of the DNA-specific dye EvaGreen for the routine quantification of DNA in microplates
    Jessica Ihrig
    Institut für Zytobiologie und Zytopathologie, Philipps Universitat Marburg, 35033 Marburg, Germany
    Anal Biochem 359:265-7. 2006
  90. ncbi [A new method in determination of formononetin content in Trifolium pratense]
    Guang wei Zhou
    Department of Chemistry, Xian Jiaotong University, Xian 710049, China
    Zhong Yao Cai 30:54-6. 2007
    ..A new method was established to determine the formononetin content in Trifoblium pratense L...
  91. ncbi Fluorometric determination of khellin in human urine and serum by high-performance liquid chromatography using postcolumn photoirradiation
    Ken Ichi Mawatari
    Department of Analytical Chemistry, Faculty of Pharmacutical Sciences, Teikyo University, Sagamiko, Kanagawa 199 0195, Japan
    Anal Sci 19:1071-3. 2003
    For the determination of khellin in urine and serum, fluorometry using HPLC-postcolumn photoirradiation has been developed. Khellin and visnagin of similar structure were separated on a column of Capcell Pak C8...
  92. ncbi Rapid high throughput assay for fluorimetric detection of doxorubicin--application of nucleic acid-dye bioprobe
    Yang Liu
    Pure and Applied Biochemistry, Lund University, P O Box 124, SE 22100 Lund, Sweden
    Anal Chim Acta 587:47-51. 2007
    ..The detection limit can be as low as 25 ng mL(-1) with an upper limit of 100 microg mL(-1). The recovery test with spiked serum sample shows that this method can be a potential routine method for therapeutic drug monitoring (TDM)...
  93. ncbi [Ca2+](i) rise in Jurkat E6-1 cell lines from different sources as a response to 50 Hz magnetic field exposure as a reproducible effect and independent of poly-L-lysine treatment
    M O Mattsson
    Department of Natural Sciences, Orebro University, Orebro, Sweden
    Cell Biol Int 25:901-7. 2001
    ..The addition of the cell adhesive peptide, poly-L-lysine, did not itself cause any effects on the intracellular calcium concentration...
  94. ncbi Non-destructive micromethod for MRP1 functional assay in human lung tumor cells
    Ekkehard Stehfest
    Institute of Environmental Toxicology, University of Halle, Franzosenweg 1a, 06097 Halle Saale, Germany
    Arch Toxicol 80:125-33. 2006
    ..These experiments confirm that MRP1 function can be followed in the attached cells in vitro under non-toxic concentrations of the substrates without the need to harvest and destroy the cells...
  95. ncbi Mutual and directional allogeneic cytotoxic reaction of hemocytes in the solitary ascidian Halocynthia roretzi revealed by one-step quantitative fluorimetric assay
    Makoto Arai
    Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba, Japan
    Zoolog Sci 19:263-70. 2002
    ..roretzi hemocytes...
  96. ncbi A fluorometric approach to local electric field measurements in a voltage-gated ion channel
    Osei Kwame Asamoah
    Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Neuron 37:85-97. 2003
    ..The extension of this method to other membrane bound proteins, including transporters, will yield insight into the role of electrical forces on protein function...
  97. ncbi Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase
    Jonathan J Goodall
    Basilea Pharmaceutica Ltd, Grenzacherstrasse 487, CH 4005 Basel, Switzerland
    Biochemistry 43:4583-91. 2004
    ..These studies indicate that histidine 20 acts as the catalytic base in the hydrolysis of peptidyl-tRNA by Pth...
  98. ncbi Calibrated automated thrombin generation in frozen-thawed platelet-rich plasma to detect hypercoagulability
    Veronique Regnault
    INSERM ERIT M 0323 et EA 3452, Faculte de Medecine, Universite Henri Poincare, CHU de Nancy, Nancy, France
    Pathophysiol Haemost Thromb 33:23-9. 2003
    ....
  99. ncbi A novel fluorometric assay for ADP-ribose pyrophosphatase activity
    Eun Kyung Song
    Department of Microbiology and Immunology, Chonbuk National University Medical School, Chonju 561 182, South Korea
    J Biochem Biophys Methods 63:161-9. 2005
    ..The fluorometric assay is comparable in sensitivity and useful for ADPRase assay in phosphate-containing samples...
  100. ncbi Fluorescence ratiometric pH sensor prepared from covalently immobilized porphyrin and benzothioxanthene
    Cheng Gang Niu
    Department of Environmental Science and Engineering, State Key Laboratory for Chemo Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China
    Anal Bioanal Chem 383:349-57. 2005
    ..0 the fluorescence intensity ratio of the two dyes varied linearly as a function of pH. The sensing membrane was found to have a lifetime of at least one month. The sensor was applied to the analysis of waste water and artificial samples...
  101. ncbi Copper(II)-selective fluorimetric bulk optode membrane based on a 1-hydroxy-9,10-anthraquinone derivative having two propenyl arms as a neutral fluorogenic ionophore
    Mojtaba Shamsipur
    Department of Chemistry, Razi University, Kermanshah, Iran
    Spectrochim Acta A Mol Biomol Spectrosc 63:43-8. 2006
    ..The proposed fluorescence optode was applied successfully to the determination of copper(II) in black tea samples...

Research Grants62

  1. Timing of Exposure to Selenium and Isoflavones and Prostate Cancer Prevention
    EDWIN DOUGLAS LEPHART; Fiscal Year: 2010
    ..Blood levels of androgen, IGF-1, and GH will be measured by ELISAs, total Se by fluorometry, and isoflavone levels by HPLC...
  2. Protein-DNA Drug Carriers for Tumor Targeting
    Lali K Medina-Kauwe; Fiscal Year: 2012
    ..We will use UV/Vis absorbance and fluorometry to assess assembly parameters and stability under different storage conditions, and in serum...
  3. Identification of Novel Small Molecules as Tau Protein Aggregation Inhibitors for
    ALAN D SNOW; Fiscal Year: 2011
    ..tau protein fibril formation and to disrupt pre-formed tau fibrils using in vitro assays including Thioflavin T fluorometry, SDS-PAGE/western blot analysis and quantitation, CD spectroscopy, and electron microscopy...
  4. MOLECULAR MECHANISMS OF RECEPTOR AND CHANNEL FUNCTION
    David Julius; Fiscal Year: 2013
    ..in vitro spectroscopic studies to examine stimulus-evoked conformational movements, and (iii) use voltage-clamp fluorometry to assess the dynamics of stimulus-evoked conformational rearrangements of TRPV1 in cells...
  5. High throughput screens for modulators of mitochondrial ATP-dependent proteolysis
    Carolyn K Suzuki; Fiscal Year: 2010
    ..primary screening assay for measuring Lonmediated degradation of reporter peptide substrate using time-resolved fluorometry. The feasibility and reproducibility of these assays will be demonstrated using commercially available chemical ..
  6. Tau Protein Aggregation Inhibitors for Tauopathies
    ALAN D SNOW; Fiscal Year: 2013
    ..This discovery has been confirmed by several independent methodologies, including Thioflavin S fluorometry, CD spectroscopy, electron microscopy and cell-based assays...
  7. Conformational rearrangements underlying ASIC1a gating
    MARCELO DANIEL CARATTINO; Fiscal Year: 2013
    ..in restricted areas of the channel following extracellular acidification will be investigated by voltage clamp fluorometry, a technique that provides information regarding local protein motion associated with specific gating steps...
  8. Mechanisms of HCN regulation by accessory subunit Trip8b using fluorescence and e
    John Bankston; Fiscal Year: 2013
    ..address these questions using a combination of fluorescence and electrophysiology that will include patch clamp fluorometry, single molecule fluorescence, and targeted mutagenesis based on crystal structures...
  9. GABA Channel Activation Domains and Mechanisms
    David S Weiss; Fiscal Year: 2013
    ..Last round, we introduced Voltage-Clamp Fluorometry to the ligand-gated ion channel field...
  10. GATING MECHANISMS OF RETINAL ROD CGMP ACTIVATED CHANNELS
    William N Zagotta; Fiscal Year: 2013
    ..for simultaneous current and fluorescence measurements from cell-free membrane patches (termed patch-clamp fluorometry, PCF)...
  11. Mechanisms of TRPV1 Channel Regulation
    William N Zagotta; Fiscal Year: 2013
    ..In this proposal we will apply standard patch-clamp methods, Patch-Clamp Fluorometry (PCF), and Transition Metal Ion FRET (tmFRET) to study purified TRPV1 channels in GUVs of defined lipid ..
  12. STRUCTURAL REARRANGEMENTS IN CHANNEL GATING
    Ehud Isacoff; Fiscal Year: 2013
    ..count the subunits of these proteins and combined these with electrophysiology to count pores and voltage clamp fluorometry to detect structural dynamics...
  13. In vivo characterization of microRNA regulation
    Jesse R Zamudio; Fiscal Year: 2011
    ..studies and tissue-specific quantification will be performed using non-invasive fluorescent microscopy and fluorometry. By crossing the miRNA reporter transgenic mouse with various cancer models for tumor and lymphoproliferative ..
  14. Quantitative MRI-based Assessment of Aerosol Deposition in the Lung
    Chantal Darquenne; Fiscal Year: 2009
    ..We will also characterize the aerosol DE patterns in the same animals by fluorometry to validate the MRI data...
  15. Molecular Mechanism of pH Regulation of TRPV1 Activation
    Jie Zheng; Fiscal Year: 2013
    ..In particular, we will apply a patch fluorometry approach to directly observe structural changes in the channel protein or the binding of regulatory molecules, ..
  16. MECHANISM OF ACTION OF NEW ANTICANCER AGENTS
    WILLIAM CREASEY; Fiscal Year: 1980
    ..uptake, distribution and metabolism of drugs will be studied by high-pressure liquid chromatography, fluorometry, and spectrophotometry...
  17. MECHANISMS OF PNEUMOCYSTIS CARINII PNEUMONIA
    William Martin; Fiscal Year: 1999
    ..by bronchoalveolar lavage will be assessed for the attachment and phagocytosis of FITC-Pc organisms by direct fluorometry, confocal laser microscopy and flow cytometry...
  18. DEVELOPMENT OF IMMUNO-PCR NEURODIAGNOSTIC KITS
    Arezoo Campbell; Fiscal Year: 2003
    ..following reaction of chromogenic substrate with digoxigenin-alkaline phosphate ligated to DNA; and (d) fluorometry of the complex produced by the action of a fluorescent substance (AttoPhos) on the DNA-digoxigenin complex...
  19. SUBUNIT INTERACTIONS IN DNA BINDING PROTEINS
    Catherine Royer; Fiscal Year: 1992
    ..hydrostatic pressure and temperature variations coupled to high sensitivity multi-frequency and steady state fluorometry. High hydrostatic pressure is known to destabilize many protein subunit interactions...
  20. PHASE-RESOLVED FLUORESCENCE STUDIES OF PROTEIN DYNAMICS
    PATRICIA O HARA; Fiscal Year: 1993
    ..motions will be examined by intramolecular nonradiative energy transfer using conventional and frequency domain fluorometry. Transfer will take place between donors and acceptors which are strategically placed fluorescent, luminescent, ..
  21. REGULATION OF HEPATIC LIPID METABOLISM BY ORGANIC ANIONS
    STEPHEN ZUCKER; Fiscal Year: 2001
    ..In additional studies, cell culture systems will be used. To this end, a series of studies using stop flow fluorometry, 13C-nmr, and fluorescence parallax studies will be performed at various pH, membrane lipid composition, and ..
  22. LIPID METABOLISM AND MEMBRANE STRUCTURE AND FUNCTION
    Michael Glaser; Fiscal Year: 1990
    ..containing membrane proteins will be studied by fluorescence polarization, energy transfer methods, and phase fluorometry. The properties of D-Beta-hydroxybutyrate dehydrogenase, cytochrome b5 and cytochrome b5 reductase will be ..
  23. MONOAMINERGIC MODULATION OF PREFRONTAL CORTEX
    DALTON SURMEIER; Fiscal Year: 2004
    ..be determined in retrogradely identified PFC pyramidal neurons using a combination of RT-PCT, voltage-clamp and fluorometry. In parallel, the modulatory effects of 5-HT-2 receptors on these same channel populations will be determined ..
  24. EFFECTS OF ANESTHETICS ON MEMBRANE HYDRATION AND FUSION
    TIMOTHY O LEARY; Fiscal Year: 1990
    ..In addition, it provides a means for localizing anesthetics within the bilayer. Fluorometry is used to assess the perturbation by general ansesthetics of calcium-induced membrane fusion, using energy ..
  25. TRANSIENT EFFECTS IN FLUORESCENCE QUENCHING
    Joseph Lakowicz; Fiscal Year: 1991
    ..We recently found that the effects can be readily detected by freqency-domain fluorometry. An important feature of the analysis is the ability to independently recover diffusion coefficients, ..
  26. CONFORMATIONAL ASPECTS OF HB BY FRONT-FACE FLUOROMETRY
    RHODA HIRSCH; Fiscal Year: 1992
    ..that the fluorescence of the tyrosine and tryptophan residues in hemoglobins can be detected by front-face fluorometry, even in the presence of hemes. This fluorescence is sensitive to quaternary structure and aggregation state...
  27. Aquaporins in Drosophila melanogaster
    Nancy Kaufmann; Fiscal Year: 2005
    ..This will be accomplished by using the yeast vesicle expression system and stopped-flow fluorometry pioneered in the Brodsky and Zeidel laboratories and, if necessary, the more traditional Xenopus laevis egg-..
  28. CALCIUM SIGNALING ACTIVATION PROCESS OF MICROGLIA CELLS
    Reinhold Penner; Fiscal Year: 2005
    ..a combination of biophysical techniques (patch-clamp electrophysiology, video microscopy, and dual-wavelength fluorometry)...
  29. METABOLIC STUDIES OF CORNEAL TISSUES
    RONALD LAING; Fiscal Year: 1992
    Clinically relevant metabolic studies of the cornea using redox fluorometry are proposed...
  30. LUMINESCENT LANTHANIDE CHELATES LABELS FOR IMMUNOASSAYS
    VISHWAS JOSHI; Fiscal Year: 1999
    ..Time-resolved fluorometry enables assays using lanthanide labels are comparable to those achieved by radioimmunoassays...
  31. Catecholamine Uptake, Micro-fluorometry/Drug Screening
    LOUIS DE FELICE; Fiscal Year: 2003
    ..The overarching goal of Aims I and II is to develop the ASP assay for high-throughput screening of putative drugs that inhibit or stimulate monoamine transport and binding, in particular the catecholamines, norepinephrine and dopamine. ..
  32. BLOOD CELL MECHANICS, SURFACE AFFINITY AND ADHESION
    Evan Evans; Fiscal Year: 1992
    ..The observations are recorded with a video microscope system. Surface binding and distribution of ligand-receptor complexes are measured with a video intensified, laser fluorometry system, coupled to the microscope system.
  33. PHOTODYNAMIC DIAGNOSTICS AS AN AID FOR CLINICAL BIOPSY
    DOUGLAS BRENNER; Fiscal Year: 1991
    ..The proposed method involves a non-laser, excitation light source and ratio fluorometry with bandpass filters and photodiodes for detection...
  34. MONAMINE TRANSPORTERS IN PARKINSONISM
    Allan Levey; Fiscal Year: 2001
    ..causes cellular damage in stable cell lines expressing different ratios of DAT/VMAT2 using microphysiology, fluorometry, and confocal microscopy. Specific aim 3...
  35. SCANNING CYTOMETRY FOR BONE MARROW PURGING
    Manfred Koller; Fiscal Year: 1999
    ..The key, components of scanning cytometry - autofocus, image segmentation., image fluorometry and a 3 Hz field scanning rate - will be combined with laser ablation to create a prototype instrument...
  36. MITOCHONDRIAL IRON METABOLISM IN AGING
    Kathleen Crowley; Fiscal Year: 1999
    ..mtATPase-1 cell line has an altered mitochondrial content and an altered cell cycle and life span by cytometry, fluorometry, and gene expression analysis...
  37. ACOUSTO-OPTIC DEVICES FOR TIME-RESOLVED FLUORESCENCE
    RONALD ROSEMEIER; Fiscal Year: 1991
    ..In recent years these phenomenan have been studied by the technique of frequency-domain fluorometry (FREDOF), which provides good signal-to-noise, ease of use, and good resolution of complex decays...
  38. Fluorescence Lifetime and Steady-State Spectrometer
    E Ostap; Fiscal Year: 2003
    ..International (PTI) TimeMaster Model 720T T-format fluorescence lifetime instrument with a steady-state fluorometry subsystem add-on which includes polarization accessories and temperature control...
  39. REDOX STATES OF NORMAL/PATHOLOGICAL CORNEA
    BARRY MASTERS; Fiscal Year: 1992
    ..The Specific Aims are: (1) to improve the spatial resolution of redox fluorometry to permit measurements on individual cells in basal, superbasal, wing and superficial epithelial cells and ..
  40. EXCITED-STATE PROPERTIES OF DNA
    Solon Georghiou; Fiscal Year: 1990
    ..the room-temperature excited-state properties of DNA and its analogs by steady-state and time- resolved fluorometry. We aim at establishing: 1. The occurrence and extent of migration of excitation energy along the double helix...
  41. Structural dynamics of the proteins involved in calcium-triggered exocytosis
    JUSTIN WORTHINGTON TARASKA; Fiscal Year: 2010
    ..Syntaxin and Syntaxin binding proteins relative to each other and to the plasma membrane with patch-clamp fluorometry;and 2) characterize the stoichiometry, dynamic associations, and functional roles of SNAREs and SNARE-..
  42. Densely Multiplexed Fluorescence Imaging by Fourier Transform Fluorometry
    Leilei Peng; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  43. Actions of Ethanol on Ligand-Gated Ion Channels
    Gustav Akk; Fiscal Year: 2006
    ..The results from the experiments will be used to examine the mechanism and structural requirements in the ethanol-mediated modulation of the GABAA receptor function. ..
  44. INSECTICIDE ACTIONS ON GABA-A RECEPTORS
    Gustav Akk; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  45. Protective Mechanism of an Anti-Inflammatory Herbal Mix
    Ali Banan; Fiscal Year: 2006
    ..abstract_text> ..
  46. Nuclease-Resistant Aptamers for Anthrax Opsonization
    John Bruno; Fiscal Year: 2004
    ..Such shielded aptamers could even be used in inhalers to enhance alveolar macrophage phagocytosis. ..
  47. Nuclease-Resistant Aptamers to Botox and Anthrax Toxins
    John Bruno; Fiscal Year: 2004
    ..Such reagents could also be used in environmental biosensors or diagnostic test kits for detection of BOTOX and anthrax toxins in body fluids or environmental samples. ..
  48. EGF, Oxidants & Intestinal Barrier Integrity
    Ali Banan; Fiscal Year: 2006
    ..abstract_text> ..
  49. Molecular Pathophysiology of HbE Diseases
    RHODA HIRSCH; Fiscal Year: 2005
    ..abstract_text> ..
  50. Molecular dynamics studies of muscle proteins
    EDWARD F PATE; Fiscal Year: 2010
    ..Improved understanding of the function of myosin V and myosin VI can be anticipated to improve therapies for Griscelli syndrome and myosin Vl-based sensorineural hearing loss in humans. ..
  51. MR IMAGING OF GENE EXPRESSION
    Ralph Weissleder; Fiscal Year: 2004
    ..The long-term goal of this research is to extend the capabilities of MR and apply it to in vivo imaging of gene expression. ..
  52. Effects of Antioxidants on Human Macular Pigments
    Kyung Jin Yeum; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  53. Purchase of a 500 MHz Solid State NMR Spectrometer
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
    ..unreadable] [unreadable] All the major users of the instrument are supported by NIH R01 grants. [unreadable] [unreadable] [unreadable]..
  54. Rapid homogeneous antibody-based detection of proteins
    Tomasz Heyduk; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  55. Membrane Binding and Imaging Applications of Annexins
    Jonathan Tait; Fiscal Year: 2007
    ..Determine which mutant proteins have faster removal from the target site, thus allowing for repeated measurement of PS expression over short periods of time (up to one day). ..
  56. Role of RALBP1 in Xenobiotic and Radiation Resistance
    Sanjay Awasthi; Fiscal Year: 2008
    ..abstract_text> ..
  57. New Perspective on Innate Antibodies in Mice
    Leonore Herzenberg; Fiscal Year: 2008
    ..abstract_text> ..
  58. Proteomic Analysis of Retinal Ganglion Cell Death in Glaucoma
    GULGUN TEZEL; Fiscal Year: 2010
    ..These studies are expected to provide comprehensive information about the nerve cell death process, thereby allowing new treatment possibilities for 3 million Americans suffering from glaucoma. ..
  59. Immuno Biosensor for Real-Time Factor V Leiden Diagnosis
    KYUNG KANG; Fiscal Year: 2005
    ..Protocols developed for the Protein C biosensor will be used to detect FVN and FVL on two individual fiber optic sensors and a prototype of dual sensor will be developed. ..