Genomes and Genes
Summary: Techniques using light resulting from PHYSICAL LUMINESCENCE emitted by LUMINESCENT PROTEINS and LUMINESCENT AGENTS.
Publications274 found, 100 shown here
- PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissuesSeung Hee Yoo
Department of Neurobiology and Physiology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
Proc Natl Acad Sci U S A 101:5339-46. 2004..These results demonstrate that peripheral tissues express self-sustained, rather than damped, circadian oscillations and suggest the existence of organ-specific synchronizers of circadian rhythms at the cell and tissue level...
- Bioluminescence imaging: progress and applicationsChristian E Badr
Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA
Trends Biotechnol 29:624-33. 2011..In this review, we discuss current trends in bioluminescence and its application in different fields with an emphasis on cancer research...
- Luminescent quantum dots for multiplexed biological detection and imagingWarren C W Chan
Department of Bioengineering, University of California at San Diego, La Jolla, CA 92093, USA
Curr Opin Biotechnol 13:40-6. 2002..These novel optical properties render quantum dots ideal fluorophores for ultrasensitive, multicolor, and multiplexing applications in molecular biotechnology and bioengineering...
- Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivoBakhos A Tannous
Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, Boston, Massachusetts 02114, USA
Nat Protoc 4:582-91. 2009..Additionally, the Gluc signal can be detected in urine and the signal can be localized in animals using in vivo bioluminescence imaging...
- Looking and listening to light: the evolution of whole-body photonic imagingVasilis Ntziachristos
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School CNY149, 13th Street 5406, Charlestown, Massachusetts 02129, USA
Nat Biotechnol 23:313-20. 2005..For photonic imaging to fully realize its potential, however, further progress will be needed in refining optical inversion methods and data acquisition techniques...
- A secreted luciferase for ex vivo monitoring of in vivo processesThomas Wurdinger
Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, 149 13th St, Charlestown, Massachusetts 02129, USA
Nat Methods 5:171-3. 2008....
- Bioluminescence imaging in mouse models quantifies beta cell mass in the pancreas and after islet transplantationJohn Virostko
Vanderbilt University, Institute of Imaging Science, Nashville, TN, USA
Mol Imaging Biol 12:42-53. 2010..We developed a mouse model that enables non-invasive assessment of changes in beta cell mass...
- Photonic detection of bacterial pathogens in living hostsC H Contag
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305 5402, USA
Mol Microbiol 18:593-603. 1995..In vivo efficacy of an antibiotic was monitored using this optical method. This study demonstrates that real time non-invasive analyses of pathogenic events and pharmacological monitoring can be performed in vivo...
- In vivo bioluminescent imaging (BLI): noninvasive visualization and interrogation of biological processes in living animalsDan M Close
The Center for Environmental Biotechnology, 676 Dabney Hall, The University of Tennessee, Knoxville, TN 37996, USA
Sensors (Basel) 11:180-206. 2011..This article will review the various bioreporter/biosensor integrations of BLI and discuss how BLI is being applied towards a new visual understanding of biological processes within the living organism...
- Non-invasive detection of a small number of bioluminescent cancer cells in vivoJae Beom Kim
Caliper Life Sciences Inc, Alameda, California, United States of America
PLoS ONE 5:e9364. 2010..Significantly higher luciferase activity in these cells allowed us to detect micrometastases in both, syngeneic Balb/c and nu/nu mice...
- Advances in in vivo bioluminescence imaging of gene expressionChristopher H Contag
Department of Pediatrics, Stanford University School of Medicine, California 94305 5208, USA
Annu Rev Biomed Eng 4:235-60. 2002..Such studies will greatly facilitate the functional analysis of a wide range of genes for their roles in health and disease...
- Tomographic bioluminescence imaging by use of a combined optical-PET (OPET) system: a computer simulation feasibility studyGeorge Alexandrakis
David Geffen School of Medicine at UCLA, Crump Institute for Molecular Imaging, University of California, 700 Westwood Plaza, Los Angeles, CA 90095, USA
Phys Med Biol 50:4225-41. 2005..The simulation results pointed out specific methodological challenges that need to be addressed before a practical implementation of OPET-based bioluminescence tomography is achieved...
- Molecular imaging of cardiac cell transplantation in living animals using optical bioluminescence and positron emission tomographyJoseph C Wu
Department of Molecular and Medical Pharmacology and Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, Calif, USA
Circulation 108:1302-5. 2003..The current method of analyzing myocardial cell transplantation relies on postmortem histology. We sought to demonstrate the feasibility of monitoring transplanted cell survival in living animals using molecular imaging techniques...
- Imaging tri-fusion multimodality reporter gene expression in living subjectsPritha Ray
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
Cancer Res 64:1323-30. 2004..Imaging of reporter gene expression from single cells to living animals with the help of a single tri-fusion reporter gene will have the potential to accelerate translational cancer research...
- Visualizing fewer than 10 mouse T cells with an enhanced firefly luciferase in immunocompetent mouse models of cancerBrian A Rabinovich
M D Anderson Cancer Center, 7455 Fannin Street, Houston, TX 77054, USA
Proc Natl Acad Sci U S A 105:14342-6. 2008..Finally, we were able to enumerate infiltrating mouse lymphocytes constituting <0.3% of total tumor cellularity, representing a significant improvement over standard methods of quantitation including flow cytometry...
- Applications of bioluminescence imaging to the study of infectious diseasesMartha Hutchens
Immunology Program, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
Cell Microbiol 9:2315-22. 2007..We expect that both the range of organisms and the molecular events able to be studied by BLI will continue to expand, yielding important insights into mechanisms of pathogenesis...
- Biocompatible luminescent silicon quantum dots for imaging of cancer cellsFolarin Erogbogbo
Department of Chemical and Biological Engineering, University at Buffalo SUNY, Buffalo, New York 14260 4200, USA
ACS Nano 2:873-8. 2008..These luminescent micelle-encapsulated Si QDs were used as luminescent labels for pancreatic cancer cells. This paves the way for silicon quantum dots to be a valuable optical probe in biomedical diagnostics...
- Self-illuminating quantum dot conjugates for in vivo imagingMin Kyung So
Molecular Imaging Program, Department of Radiology, Stanford University, 1201 Welch Road, Stanford, California 94305 5484, USA
Nat Biotechnol 24:339-43. 2006..Compared with existing quantum dots, self-illuminating quantum dot conjugates have greatly enhanced sensitivity in small animal imaging, with an in vivo signal-to-background ratio of > 10(3) for 5 pmol of conjugate...
- The natural stilbenoid pinosylvin and activated neutrophils: effects on oxidative burst, protein kinase C, apoptosis and efficiency in adjuvant arthritisViera Jancinova
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic
Acta Pharmacol Sin 33:1285-92. 2012....
- Advances in bioluminescence imaging of live animal modelsRobin S Dothager
Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, MO 63110, USA
Curr Opin Biotechnol 20:45-53. 2009..New approaches to bioluminescence imaging of cell migration, signaling pathways, drug action, and interacting protein partners in vivo allow the study of biology and disease within the context of living animals...
- In vivo imaging of light-emitting probesB W Rice
Xenogen Corporation, 860 Atlantic Avenue, Alameda, CA 94501, USA
J Biomed Opt 6:432-40. 2001..Instrumentation for in vivo imaging developed at Xenogen is described and several examples of images of mice with bioluminescent cells are presented...
- In vivo imaging of ligand receptor binding with Gaussia luciferase complementationKathryn E Luker
Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan, USA
Nat Med 18:172-7. 2012..We expect this imaging technology to advance research in areas such as ligand-receptor interactions and the development of new therapeutic agents in cell-based assays and small animals...
- Imaging of bubonic plague dynamics by in vivo tracking of bioluminescent Yersinia pestisToan Nham
Yersinia Research Unit, Institut Pasteur, Paris, France
PLoS ONE 7:e34714. 2012..This highlights the extraordinary capacity of Y. pestis to annihilate the host innate immune response...
- Quantitative comparison of the sensitivity of detection of fluorescent and bioluminescent reporters in animal modelsTamara Troy
Xenogen Corporation, USA
Mol Imaging 3:9-23. 2004..The use of blue-shifted excitation filters is explored as a method to subtract out tissue autofluorescence and improve the sensitivity of fluorescent imaging...
- Bioluminescent imaging (BLI) to improve and refine traditional murine models of tumor growth and metastasisDarlene E Jenkins
Xenogen Corporation, Alameda, California 94501, USA
Clin Exp Metastasis 20:733-44. 2003..The ability to temporally assess tumor development and responses to drug therapies in vivo also improves upon current standard animal models that are based on single end point data...
- Source reconstruction for spectrally-resolved bioluminescence tomography with sparse a priori informationYujie Lu
David Geffen School of Medicine at UCLA, University of California, Los Angeles, CA 90095, USA
Opt Express 17:8062-80. 2009..Experimental reconstruction with a mouse-shaped phantom further shows the potential of the proposed algorithm...
- In vivo bioluminescence imaging of the murine pathogen Citrobacter rodentiumSiouxsie Wiles
Division of Cell and Molecular Biology, Faculty of Natural Sciences, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom
Infect Immun 74:5391-6. 2006..In addition, BLI revealed that C. rodentium colonizes the rectum, a site previously unreported for this pathogen...
- A highly sensitive protein-protein interaction assay based on Gaussia luciferaseIngrid Remy
Departement de Biochimie, Universite de Montreal, C P 6128, Succursale Centre Ville, Montreal, Quebec, H3C 3J7, Canada
Nat Methods 3:977-9. 2006..We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGFbeta signaling...
- Bioluminescent imaging: a critical tool in pre-clinical oncology researchKaren O'Neill
UCD School of Medicine and Medical Science, Health Science Building, University College Dublin, Belfield, Dublin 4, Ireland
J Pathol 220:317-27. 2010..Such novel studies highlight the versatility and potential of bioluminescent imaging in future oncological research...
- Novel genetically encoded biosensors using firefly luciferaseFrank Fan
ACS Chem Biol 3:346-51. 2008..These and other luminescent biosensors will be useful for the sensitive detection of cellular physiology in research and drug discovery...
- Noninvasive bioluminescence imaging of herpes simplex virus type 1 infection and therapy in living miceGary D Luker
Molecular Imaging Center, Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Virol 76:12149-61. 2002..These data demonstrate that bioluminescence imaging can be used for noninvasive, real-time monitoring of HSV-1 infection and therapy in living mice...
- Kinetic analysis and modeling of firefly luciferase as a quantitative reporter gene in live mammalian cellsJolene M Ignowski
Department of Chemical Engineering and the Helen Wills Neuroscience Institute, MC 1462, University of California Berkeley, Berkeley, CA 94720 1462, USA
Biotechnol Bioeng 86:827-34. 2004..Finally, we have modeled luciferase activity in live cells for the purposes of understanding and translating the luciferase signal into a more effective metric of gene expression and cell behavior...
- Structural basis for the spectral difference in luciferase bioluminescenceToru Nakatsu
Kinetic Crystallography Research Team, Membrane Dynamics Research Group, RIKEN Harima Institute at SPring 8, 1 1 1 Kouto, Mikazuki cho, Sayo gun, Hyogo 679 5148, Japan
Nature 440:372-6. 2006..Our results indicate that the degree of molecular rigidity of the excited state of oxyluciferin, which is controlled by a transient movement of Ile 288, determines the colour of bioluminescence during the emission reaction...
- Trafficking mesenchymal stem cell engraftment and differentiation in tumor-bearing mice by bioluminescence imagingHui Wang
Department of Radiology, Bio X Program, Stanford University School of Medicine, Stanford, California 94305 5484, USA
Stem Cells 27:1548-58. 2009..The MSCs-R migrated to lung tumor differentiated into osteoblasts, whereas the MSCs-R targeting subcutaneous tumor differentiated into adipocytes...
- Bioluminescence imaging: looking beyond the lightMarleen Keyaerts
In Vivo Cellular and Molecular Imaging ICMI Laboratory, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, 1090 Brussels, Belgium
Trends Mol Med 18:164-72. 2012..The knowledge and detection of such factors, together with the development of new strategies and better BLI compounds, will improve the accuracy of the technique in the future...
- Noninvasive monitoring of β-cell mass and fetal β-cell genesis in mice using bioluminescence imagingYukari Sekiguchi
Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki 305 8575, Japan
Exp Anim 61:445-51. 2012..In summary, we show that bioluminescence imaging of mice expressing a β-cell specific reporter allows detection of changes in β-cell mass and visualization of fetal β-cell neogenesis in uteri...
- Cardiac atrial circadian rhythms in PERIOD2::LUCIFERASE and per1:luc mice: amplitude and phase responses to glucocorticoid signaling and medium treatmentDaan R van der Veen
Department of Biological Sciences, University of Notre Dame, Indiana, United States of America
PLoS ONE 7:e47692. 2012..The current data describe novel entrainment signals for the atrial circadian clock and specifically highlight entrainment by mechanical treatment, an intriguing observation considering the mechanical nature of cardiac tissue...
- Dual-wavelength imaging of tumor progression by activatable and targeting near-infrared fluorescent probes in a bioluminescent breast cancer modelBang Wen Xie
Department of Endocrinology and Metabolic Diseases, Leiden University Medical Center, Leiden, The Netherlands
PLoS ONE 7:e31875. 2012..This study may open up new perspectives for the detection of tumors and metastases in various experimental models and could also have clinical applications, such as image-guided surgery...
- In vivo bioluminescent imaging of influenza a virus infection and characterization of novel cross-protective monoclonal antibodiesNicholas S Heaton
Icahn School of Medicine at Mount Sinai, Department of Microbiology, New York, New York, USA
J Virol 87:8272-81. 2013..The use of luciferase reporter influenza viruses allows for new mechanistic studies to expand our knowledge of virus-induced disease and provides a new quantitative method to evaluate future antiviral therapies. ..
- Evaluation of impaired beta-cell function in nonobese-diabetic (NOD) mouse model using bioluminescence imagingDror Sever
Department of Endocrinology, Hadassah University Hospital, P O Box 12000, 91120 Jerusalem, Israel
FASEB J 25:676-84. 2011....
- Autoluminescent Mycobacterium tuberculosis for rapid, real-time, non-invasive assessment of drug and vaccine efficacyTianyu Zhang
Center for Tuberculosis Research, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
PLoS ONE 7:e29774. 2012..Use of this autoluminescent reporter strain has the potential to drastically reduce the time, effort, animals and costs consumed in the evaluation of drug activity in vitro and the in vivo assessment of drug and vaccine efficacy...
- Iridium(III) complex-coated nanosystem for ratiometric upconversion luminescence bioimaging of cyanide anionsJinliang Liu
Department of Chemistry, Fudan University, 220 Handan Road, Shanghai 200433, PR China
J Am Chem Soc 133:15276-9. 2011..The UCL probe provides a very low detection limit of 0.18 μM CN(-) in the aqueous solution...
- Temporal variations of skin pigmentation in C57BL/6 mice affect optical bioluminescence quantitationAllison Curtis
Global Imaging Group, Novartis Institutes for BioMedical Research, Cambridge, MA 02139, USA
Mol Imaging Biol 13:1114-23. 2011....
- Rapid measurement of antituberculosis drug activity in vitro and in macrophages using bioluminescenceNuria Andreu
Microbiology, Department of Medicine, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK
J Antimicrob Chemother 67:404-14. 2012..The aim of this work was to validate and standardize the use of luminescent M. tuberculosis strains to test the activity of antibacterial drugs in vitro and inside macrophages in a 96-well format...
- In vivo bioluminescence imaging for integrated studies of infectionTimothy C Doyle
Molecular Imaging Program at Stanford MIPS, Clark Center, Bio X Program, 318 Campus Drive, Room E 150, Stanford University School of Medicine, Stanford, CA 94305 5427, USA
Cell Microbiol 6:303-17. 2004....
- Self-renewal and expansion of single transplanted muscle stem cellsAlessandra Sacco
Baxter Laboratory in Genetic Pharmacology, Department of Microbiology and Immunology, Stem Cell Institute, Stanford University School of Medicine, Stanford, California 94305 5175, USA
Nature 456:502-6. 2008....
- Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitroGregory Michel
Universite de Nice Sophia Antipolis, Faculte de Medecine, Nice, France
PLoS Negl Trop Dis 5:e1323. 2011..This model further proved to be valuable for in vivo measurement of the efficiency of drugs such as miltefosine and may, therefore, additionally be used to evaluate vaccine-induced protection...
- A highly selective, label-free, homogenous luminescent switch-on probe for the detection of nanomolar transcription factor NF-kappaBDik Lung Ma
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
Nucleic Acids Res 39:e67. 2011..This will allow the rapid and low cost identification and development of novel scaffolds for the treatment of diseases caused by the deregulation of transcription factor activity...
- Imaging cardiac stem cell therapy: translations to human clinical studiesWendy Y Zhang
Department of Medicine Division of Cardiology, Stanford University School of Medicine, 300 Pasteur Drive, Grant S140, Stanford, CA 94305 5111, USA
J Cardiovasc Transl Res 4:514-22. 2011..We then discuss recent clinical studies that have utilized these modalities to gain biological insights into stem cell fate...
- In vivo quantitative assessment of cell viability of gadolinium or iron-labeled cells using MRI and bioluminescence imagingJamal Guenoun
Department of Radiology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
Contrast Media Mol Imaging 8:165-74. 2013..These findings clearly indicate Gd to be the favourable contrast agent in qualitative and quantitative evaluation of labeled cell fate in future cell therapy experiments...
- Dual-color upconversion fluorescence and aptamer-functionalized magnetic nanoparticles-based bioassay for the simultaneous detection of Salmonella Typhimurium and Staphylococcus aureusNuo Duan
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Anal Chim Acta 723:1-6. 2012....
- Phosphorescence imaging of homocysteine and cysteine in living cells based on a cationic iridium(III) complexLiqin Xiong
Department of Chemistry, Fudan University, Shanghai 200433, PR China
Inorg Chem 49:6402-8. 2010..Importantly, cell imaging experiments demonstrated that the probe is membrane permeable and can monitor the changes of Hcy/Cys within living cells in a ratiometric mode...
- Mouse model of chronic post-arthroplasty infection: noninvasive in vivo bioluminescence imaging to monitor bacterial burden for long-term studyJonathan R Pribaz
Orthopaedic Hospital Research Center, Orthopaedic Hospital Department of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles UCLA, USA
J Orthop Res 30:335-40. 2012....
- Fusion of Gaussia luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imagingKaty M Venisnik
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Mol Imaging Biol 9:267-77. 2007..4% ID/g) at 21 hours. Although further optimization of this fusion protein may be needed to improve in vivo performance, the diabody-GLDelta15 is a promising optical imaging probe for tumor detection in vivo...
- Self-illuminating in vivo lymphatic imaging using a bioluminescence resonance energy transfer quantum dot nano-particleNobuyuki Kosaka
Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892 1088, USA
Contrast Media Mol Imaging 6:55-9. 2011..These advantages of BRET-QDot allowed us to perform real-time, quantitative lymphatic imaging without image processing. BRET-Qdots have the potential to be a robust nano-material platform for developing optical molecular imaging probes...
- Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imagingBradley A Creamer
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, Nebraska 68198 6805, USA
Genesis 47:234-45. 2009..The study shows that, unlike their progeny in mammary epithelial transplants as reported previously, PI-MECs themselves may not belong to the long-term label-retaining epithelial subtype...
- Enhanced beetle luciferase for high-resolution bioluminescence imagingYoshihiro Nakajima
National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan
PLoS ONE 5:e10011. 2010..These results demonstrate that the use of ELuc allows a BLI spatiotemporal resolution far greater than that provided by FLuc...
- On the potential for molecular imaging with Cerenkov luminescenceMatthew A Lewis
Advanced Radiological Sciences, Department of Radiology, UT Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9058, USA
Opt Lett 35:3889-91. 2010....
- Clinical potential of intravenous neural stem cell delivery for treatment of neuroinflammatory disease in mice?Kristien P Reekmans
Laboratory of Experimental Hematology, University of Antwerp, Belgium
Cell Transplant 20:851-69. 2011..However, given the fact that grafted NSCs have an intrinsic survival capacity in the CNS, their therapeutic exploitation should be further investigated, and-in contrast to several other reports-will most likely be highly complex...
- Multilevel bioluminescence tomography based on radiative transfer equation part 2: total variation and l1 data fidelityHao Gao
Department of Mathematics, University of California, Irvine, CA 92697, USA
Opt Express 18:2894-912. 2010....
- Reporter gene-expressing bone marrow-derived stromal cells are immune-tolerated following implantation in the central nervous system of syngeneic immunocompetent miceIrene Bergwerf
Laboratory of Experimental Hematology, University of Antwerp, Antwerp, Belgium
BMC Biotechnol 9:1. 2009..However, in many pre-clinical cell therapy studies, reporter gene-assisted imaging of cellular implants in the CNS and potential reporter gene and/or cell-based immunogenicity, still remain challenging research topics...
- Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imagingMatthias Edinger
Division of Bone Marrow Transplantation, Department of Medicine, Stanford University School of Medicine, CA, USA
Blood 101:640-8. 2003..The complex cellular processes in bone marrow transplantation and antitumor immunotherapy, previously inaccessible to investigation, can now be revealed in real time in living animals...
- Dynamic bioluminescence imaging for quantitative tumour burden assessment using IV or IP administration of D: -luciferin: effect on intensity, time kinetics and repeatability of photon emissionMarleen Keyaerts
In Vivo Cellular and Molecular Imaging ICMI Laboratory, Vrije Universiteit Brussel VUB, Brussels, Belgium
Eur J Nucl Med Mol Imaging 35:999-1007. 2008..The aim of this study is to compare the repeatability of BLI measurement after IV versus IP administration of D: -luciferin and assess the correlation between photon emission and histological cell count both in vitro and in vivo...
- Phosphorescent light-emitting iridium complexes serve as a hypoxia-sensing probe for tumor imaging in living animalsShaojuan Zhang
Secretion Biology Laboratory, Institute for Molecular and Cellular Regulation and Division of Diagnostic Radiology and Nuclear Medicine, Graduate School of Medical Sciences, Gunma University, Maebashi, Japan
Cancer Res 70:4490-8. 2010..One of them, BTPHSA, depicted clear imaging from tumors transplanted 6 to 7 mm deep from the skin surface. We suggest that iridium complex materials have a vast potential for imaging hypoxic lesions such as tumor tissues...
- Hyperspectral and multispectral bioluminescence optical tomography for small animal imagingAbhijit J Chaudhari
Signal and Image Processing Institute, Department of Electrical Engineering Systems, University of Southern California, Los Angeles, 90089, USA
Phys Med Biol 50:5421-41. 2005..Co-registration of the reconstructed 3D bioluminescent image with magnetic resonance images indicated good anatomical localization of the tumour...
- Two new "protected" oxyphors for biological oximetry: properties and application in tumor imagingTatiana V Esipova
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States
Anal Chem 83:8756-65. 2011..These experiments revealed that changes in isofluorane concentration significantly affect tissue oxygenation...
- Uniqueness theorems in bioluminescence tomographyGe Wang
Bioluminescence Tomography Laboratory, Departments of Radiology, Biomedical Engineering, and Mathematics, University of Iowa, Iowa City, Iowa 52242, USA
Med Phys 31:2289-99. 2004..For better readability, the exact conditions for and rigorous proofs of the theorems are given in the Appendices. Further research directions are also discussed...
- Bioluminescent Leishmania expressing luciferase for rapid and high throughput screening of drugs acting on amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living miceThierry Lang
Unité d Immunophysiologie et Parasitisme Intracellulaire, Departement de Parasitologie, Institut Pasteur, rue 25 du Dr Roux, 75724 Paris Cedex 15, France
Cell Microbiol 7:383-92. 2005..In short, this luciferase imaging study is useful to monitor the efficacy of anti-leishmanial drugs on live cell culture and to trace leishmanial infection in animal models...
- Red- and green-emitting firefly luciferase mutants for bioluminescent reporter applicationsBruce R Branchini
Department of Chemistry, Connecticut College, New London, CT 06320, USA
Anal Biochem 345:140-8. 2005..The results presented here with the P. pyralis mutants provide evidence that simultaneous multiple analyte assay development is feasible with these novel proteins that require only a single substrate...
- Optical imaging of Cerenkov light generation from positron-emitting radiotracersR Robertson
Department of Imaging Sciences, Millennium Pharmaceuticals, Inc, Cambridge, MA 02139, USA
Phys Med Biol 54:N355-65. 2009....
- Bioluminescent imaging of Trypanosoma cruzi infectionKenneth V Hyland
Department of Microbiology Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA
Int J Parasitol 38:1391-400. 2008....
- Increase in bioluminescence intensity of firefly luciferase using genetic modificationHiroya Fujii
Bussan Nanotech Research Institute Inc, Tsukuba, Ibaraki 305 0074, Japan
Anal Biochem 366:131-6. 2007..Combining these mutations, we obtained luciferases that generate more than 10-fold higher luminescence intensity than the wild-type enzyme...
- Inhibitory effect of stobadine on FMLP-induced chemiluminescence in human whole blood and isolated polymorphonuclear leukocytesKatarina Drabikova
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Dubravska cesta 9, 841 04 Bratislava, Slovak Republic
Luminescence 22:67-71. 2007....
- In vivo bioluminescence imaging of cord blood derived mesenchymal stem cell transplantation into rat myocardiumJung Joon Min
Institute for Molecular Photonic Imaging Research, Chonnam National University Hospital, Republic of Korea
Ann Nucl Med 20:165-70. 2006..Here, we have sought to demonstrate the feasibility of a longitudinal monitoring of transplanted cell survival in living animals, accomplished with optical imaging techniques and pharmacological interventions...
- Naturally appearing N-feruloylserotonin isomers suppress oxidative burst of human neutrophils at the protein kinase C levelRado Nosal
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska 9, 841 04 Bratislava, Slovak Republic
Pharmacol Rep 63:790-8. 2011..It should be further investigated for its pharmacological activity against oxidative stress in ischemia-reperfusion, inflammation and other pathological conditions...
- Sensitive dual color in vivo bioluminescence imaging using a new red codon optimized firefly luciferase and a green click beetle luciferaseLaura Mezzanotte
Department of Pharmaceutical Sciences, University of Bologna, Bologna, Italy
PLoS ONE 6:e19277. 2011..A new red emitting codon-optimized luciferase reporter gene mutant of Photinus pyralis, Ppy RE8, has been developed and used in combination with the green click beetle luciferase, CBG99...
- Luciferin derivatives for enhanced in vitro and in vivo bioluminescence assaysRajesh Shinde
Department of Pediatrics, Radiology, and Microbiology and Immunology, Stanford University, Clark Center, 318 Campus Drive, Stanford, California 94305, USA
Biochemistry 45:11103-12. 2006..The ability to assay luciferase in vitro and in vivo using these substrates, which exhibit different pharmacokinetic and pharmacodynamic properties, will provide flexibility and improve current imaging capabilities...
- Uptake kinetics and biodistribution of 14C-D-luciferin--a radiolabeled substrate for the firefly luciferase catalyzed bioluminescence reaction: impact on bioluminescence based reporter gene imagingFrank Berger
Department of Clinical Radiology, Ludwig Maximilians University Munich, Munich, Germany
Eur J Nucl Med Mol Imaging 35:2275-85. 2008..Biokinetics and distribution of the substrate most likely have a significant impact on levels of light signal and therefore need to be investigated...
- Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesisKozo Hirokawa
Research and Development Division, Kikkoman Corporation, 399 Noda, Chiba Prefecture 278 0037, Japan
Biochim Biophys Acta 1597:271-9. 2002..cruciata luciferase. The pH-dependence of the bioluminescence emission spectra was also examined. In contrast to wild-type firefly luciferases characterized to date, the mutant did not show the pH-dependent red spectrum shift...
- Intrinsic, nondeterministic circadian rhythm generation in identified mammalian neuronsAlexis B Webb
Department of Biology, Washington University, St Louis, MO 63130, USA
Proc Natl Acad Sci U S A 106:16493-8. 2009..Instead, these results indicate that AVP, VIP, and other SCN neurons are intrinsic but unstable circadian oscillators that rely on network interactions to stabilize their otherwise noisy cycling...
- Validating bioluminescence imaging as a high-throughput, quantitative modality for assessing tumor burdenZain Paroo
The University of Texas Southwestern Medical Center at Dallas, 75390 9058, USA
Mol Imaging 3:117-24. 2004....
- Cerenkov luminescence tomography for small-animal imagingChangqing Li
Department of Biomedical Engineering, University of California, Davis, Davis, California 95616, USA
Opt Lett 35:1109-11. 2010....
- Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in miceBlandine Franke-Fayard
Malaria Research Group, Department of Parasitology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
Nat Protoc 1:476-85. 2006..Real-time in vivo imaging of infected erythrocytes will provide increased insights into the dynamics of sequestration and its role in pathology, and can be used to evaluate strategies that prevent sequestration...
- Guided by the light: visualizing biomolecular processes in living animals with bioluminescenceJennifer A Prescher
Molecular Imaging Program at Stanford, Stanford School of Medicine, Stanford, CA, USA
Curr Opin Chem Biol 14:80-9. 2010..Collectively, these efforts are expanding our understanding of living systems and shedding light on the molecular underpinnings of disease...
- An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plantsElmar Schmälzlin
Institute of Chemistry, University of Potsdam, Germany
Biophys J 89:1339-45. 2005..The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species...
- RAP, an integrated program for monitoring bioluminescence and analyzing circadian rhythms in real timeKazuhisa Okamoto
Center for Gene Research, Nagoya University, Furo, Chikusa, Nagoya 464 8602, Japan
Anal Biochem 340:193-200. 2005..The program is a powerful tool for large-scale investigations of biological rhythmic phenomena in general...
- Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyiJennifer M Henke
Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
J Bacteriol 186:6902-14. 2004..Together these communication systems act as a three-way coincidence detector in the regulation of a variety of genes, including those responsible for bioluminescence, type III secretion, and metalloprotease production...
- Multilevel bioluminescence tomography based on radiative transfer equation Part 1: l1 regularizationHao Gao
Department of Mathematics, University of California, Irvine, CA 92697, USA
Opt Express 18:1854-71. 2010..Besides, we study and compare reconstructions with boundary angular-averaged data, boundary angular-resolved data and internal angular-averaged data respectively...
- Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome c reductionFlorence Caldefie-Chezet
Laboratoire de Biochimie, Biologie Moléculaire et Nutrition, EA 2416, Centre de Recherche en Nutrition Humaine, Faculte de Pharmacie, 28 place Henri Dunant B P 38, 63001 Clermont Ferrand Cedex 1, France
Clin Chim Acta 319:9-17. 2002..Several methods of ROS detection are commonly used. However, the literature shows that it is not always clear whether the species detected are IC or EC, especially with the chemiluminescence technique...
- Direct continuous method for monitoring biofilm infection in a mouse modelJagath L Kadurugamuwa
Xenogen Corp, Alameda, California 94501, USA
Infect Immun 71:882-90. 2003....
- Selective killing of hypoxia-inducible factor-1-active cells improves survival in a mouse model of invasive and metastatic pancreatic cancerShinae Kizaka-Kondoh
Innovative Techno Hub for Integrated Medical Bio Imaging and Department of Radiation Oncology and Image Applied Therapy, Kyoto University Graduate School of Medicine, Kyoto, Japan
Clin Cancer Res 15:3433-41. 2009..However, the behavior of the cells having HIF-1 activity during the malignant progression in pancreatic cancer has not been tested...
- Noninvasive and quantitative monitoring of adult neuronal stem cell migration in mouse brain using bioluminescence imagingVeerle Reumers
Neurobiology and Gene Therapy, Katholieke Universiteit Leuven, Leuven, Flanders, Belgium
Stem Cells 26:2382-90. 2008..Long-term expression of brain-derived neurotrophic factor in the subventricular zone attenuated neurogenesis, as detected by histology and BLI. In vivo monitoring of the impact of drugs or genes on adult neurogenesis is now within reach...
- Bioluminescent CXCL12 fusion protein for cellular studies of CXCR4 and CXCR7Kathryn Luker
Center for Molecular Imaging, Department of Radiology, University of Michigan Medical School, Ann Arbor, MI, USA
Biotechniques 47:625-32. 2009..These data validate CXCL12-GL as a bioluminescent probe to investigate molecular functions of CXCR4 and CXCR7 and screen for compounds that modulate ligand-receptor binding...
- Heterogeneous response to a quorum-sensing signal in the luminescence of individual Vibrio fischeriPablo Delfino Pérez
Physics Department, University of Florida, Gainesville, Florida, United States of America
PLoS ONE 5:e15473. 2010..The observed heterogeneity shows that although a given concentration of quorum signal may determine the average light output from a population of cells, it provides far weaker control over the luminescence output of each individual cell...
- Analysis of intracellular oxygen and metabolic responses of mammalian cells by time-resolved fluorometryAlexander V Zhdanov
Luxcel Biosciences Ltd, BioInnovation Centre, UCC, Cork, Ireland
Anal Chem 79:9414-9. 2007..The assay provides relevant, information-rich data on cellular function and metabolism. It allows monitoring of small, rapid, and transient changes in cell respiration and screening of new chemical entities...
- Phosphorescent chemosensors based on heavy-metal complexesQiang Zhao
Department of Chemistry, Fudan University, Shanghai 200433, PR China
Chem Soc Rev 39:3007-30. 2010....
- A rapid luminescent assay for measuring cytochrome P450 activity in individual larval Culex pipiens complex mosquitoes (Diptera: Culicidae)A B Inceoglu
Department of Entomology, University of California, Davis, CA 95616, USA
J Med Entomol 46:83-92. 2009..The assay platform is highly flexible in terms of choice of tissue, method of preparation, isozyme specificity, and sample quantity and thus could easily be adapted to be used for other arthropod species...
- Evaluation of biophotonic imaging to estimate bacterial burden in mice infected with highly virulent compared to less virulent Streptococcus pneumoniae serotypesStefanie Henken
Department of Experimental Pneumology, Hannover School of Medicine, Hannover 30625, Germany
Antimicrob Agents Chemother 54:3155-60. 2010..pneumoniae-induced lung infections in mice is largely influenced by the disease model under investigation. The provided data may be important for studies of infectious diseases...
- Optical imaging of tumor cells in hollow fibers: evaluation of the antitumor activities of anticancer drugs and target validationGuo Jun Zhang
Imaging Department, MRL, Merck and Co, Inc, West Point, PA 19486, USA
Neoplasia 9:652-61. 2007..These results demonstrate that optical imaging of hollow fibers containing reporter tumor cells can be used for the rapid and accurate evaluation of antitumor activities of anticancer drugs and for measurement of molecular pathways...
- Intracellular oxygen-sensitive phosphorescent probes based on cell-penetrating peptidesRuslan I Dmitriev
Biochemistry Department, University College Cork, Cavanagh Pharmacy Building, Cork, Ireland
Anal Biochem 398:24-33. 2010..They provide a simple and versatile tool for O(2) monitoring in live cells and in tissue, and an alternative to the existing O(2) probes which require facilitated transport into the cell...
- Imaging of oxygen gradients in monolayer cultured cells using green fluorescent proteinEiji Takahashi
Biomedical Engineering Course, Advanced Technology Fusion, Graduate School of Science and Engineering, Saga Univ, Saga 840 8502, Japan
Am J Physiol Cell Physiol 299:C1318-23. 2010..Findings in the present two-dimensional O(2) diffusion model support the crucial role of tissue O(2) diffusion in defining the O(2) microenvironment in individual cells...
- Conformations and interactions of insect olfactory receptor proteinsROBERT D RENTHAL; Fiscal Year: 2010..Also, one component of the insect pheromone receptor is similar to human proteins involved in atherosclerosis, inflammatory response, metabolic syndrome, cardiomyopathies, high frequency hearing loss, and Gaucher's disease. ..
- Rapid Kinase Profiling with Luminescent ReportersReena Zutshi; Fiscal Year: 2012..Our goal is to make these profiling assays affordable, so that compound profiling can be done earlier leading to early identification of failures and resulting in many more opportunities for success. ..
- OXYGEN LEVELS AND RADIOSENSITIVITY IN TUMORSDavid Wilson; Fiscal Year: 2001..Phosphors will be selected which can be injected intravenously and then, after the oxygen measurements, are excreted by the kidney. ..
- Therapeutics targeting fatty acid synthesis in P. aeruginosaDonald Moir; Fiscal Year: 2009..Inhibitors will be verified to act on this pathway by testing them in a biochemical assay of the entire fatty acid synthesis pathway. ..
- DETECTION OF MACROMOLECULES WITH QUANTUM DYESRobert Leif; Fiscal Year: 2000..PROPOSED COMMERCIAL APPLICATION: Proposed commercial application not available. ..
- BIOMEDICAL RESEARCH SUPPORTNicholas Kefalides; Fiscal Year: 1991....
- Identifying targets for diagnosing oral candidiasisDavid Schofield; Fiscal Year: 2005..Since Candida species are the most important fungal pathogen of the oral cavity, the rapid and accurate diagnosis of oral candidiasis using novel disease biomarkers will significantly improve oral health. ..
- DOPAMINE/GLUTAMATE AND HYPOXIC INJURY IN NEWBORN BRAINDavid Wilson; Fiscal Year: 2009..This understanding will be the basis for more effective treatment of patients who have been subjected to a period(s) of insufficient oxygen delivery to the brain. ..
- Nanosensor probes for oxygen imaging in vivoDavid Wilson; Fiscal Year: 2009..e. ready for larger scale synthesis and rigorous toxicology studies preparatory to use in clinical trials. ..
- Imaging Vaccina Viral-Host PathogenesisGary Luker; Fiscal Year: 2007..Collectively, the research is expected to facilitate the development of improved vaccines and treatments for smallpox, thereby enhancing our ability to prevent the use of smallpox for bioterrorism. [unreadable] [unreadable] [unreadable]..
- Center for Fetal Gene Transfer for HLB DiseasesAlice Tarantal; Fiscal Year: 2005..abstract_text> ..
- Center for Pediatric Stem/Progenitor Cell Translational ResearchAlice Tarantal; Fiscal Year: 2008..If imaging techniques can be developed that allows one to monitor the cells over time, this would provide a very powerful tool to determine the fate of the cells after injection into humans. ..
- Mesenchymal Stem Cells for Fetal Gene DeliveryAlice Tarantal; Fiscal Year: 2006..abstract_text> ..
- Noninvsive Imaging of Chorio-retinal Oxygen TensionMahnaz Shahidi; Fiscal Year: 2005..abstract_text> ..
- Retinal Image Quality in Retinal-diseased EyesMahnaz Shahidi; Fiscal Year: 2010....
- Caspases, Calpains and Neuronal Cell deathStan Krajewski; Fiscal Year: 2006..These animals and cells derived from them will then be used for determining the importance of these proteases for neuronal cell death induced by cerebral ischemia and in certain neurodegenerative diseases. ..
- Molecular Imaging of Cardiac Cell TransplantationJoseph Wu; Fiscal Year: 2008..The goal is to use molecular imaging to guide stem cell therapy, which should translate into transplant protocols that are more reproducible, quantifiable, and beneficial. ..
- Nanostructuring and Molecular Imaging of Engineered Cardiovascular TissuesJoseph Wu; Fiscal Year: 2008..With a multi-disciplinary team approach, we are confident that these combined efforts will accelerate the translation of nanotechnology-based tissue engineering to the clinical arena in the future. ..
- Glycophorin A Dimers to Probe Membranes: A 2D-IR StudyLOUISE SINKS; Fiscal Year: 2008..Since many transmembrane proteins critical to cell function, understanding the interplay between membrane environment and protein structure is vitallv important. ..
- Reverse Transcription Free Microarray AnalysisYe Sun; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Mixture Toxicity - Evaluation of an Improved MethodologyDOUGLAS DAWSON; Fiscal Year: 2008..Environmental releases of these chemicals are possible, which could also affect public health. [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
- BIOSENSOR ARRAYS BASED ON SINGLE WALL CARBON NANOTUBESJames F Rusling; Fiscal Year: 2010..In addition to achieving our specific aims, this project should provide fundamental guidance for fabrication of sensitive microarray devices for a wide variety of biomedical applications. ..
- Risk for New onset of Depression in Perimenopausal WomenDaniel Cramer; Fiscal Year: 2009..abstract_text> ..
- Continuous Sequencing-By-Synthesis Based on a Digital Microfluidic PlatformRICHARD FAIR; Fiscal Year: 2009....
- MUC-1 related cancer immunity: determinants and predictive significanceDaniel Cramer; Fiscal Year: 2009..The findings of our study could provide the basis for developing a safe cancer- preventing vaccine for ovarian cancer and perhaps other MUC1-expressing tumors. ..
- Amyloid beta Peptide and Apolipoprotein EThomas Wisniewski; Fiscal Year: 2009..These studies will test our central hypothesis that blocking the Aa/apoE interaction can serve as a novel, non-toxic therapeutic target for Alzheimer's disease. ..
- ENZYMATIC LUMINESCENCE microRNA ASSAYYe Sun; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Immunization Approaches for Alzheimer's DiseaseThomas Wisniewski; Fiscal Year: 2008..Vaccination is potentially an effective treatment, but is associated with significant toxicity in about 6% of patients. We propose studies to help develop safe, effective vaccines. [unreadable] [unreadable] [unreadable]..
- EPIDEMIOLOGIC AND BIOLOGIC PREDICTORS OF IVF SUCCESSDaniel Cramer; Fiscal Year: 2002....
- DENDRITIC O2 SENSOR WITH TWO-PHOTON ABSORBING ANTENNASergei Vinogradov; Fiscal Year: 2005..If successful, these experiments will open avenues for novel research, which will span from the studies of protein conformations by small molecule diffusion to the construction of a single-molecule oxygen sensor. ..
- Beta-Cell Mass And Function In Implants and PancreasWen Hong Li; Fiscal Year: 2003..abstract_text> ..
- Luminescence Studies of Lanthanide Contrast AgentsJANET MORROW; Fiscal Year: 2006..The new laser system will also be available to researchers throughout the country and will be at the heart of a center for Ln(lll) luminescence research. [unreadable] [unreadable] [unreadable]..
- Non-Invasive Imaging of T Cell TraffickingBernd Pichler; Fiscal Year: 2006..abstract_text> ..
- Nanoparticle-Based Optical Sensor for Glucose MonitoringRalph Ballerstadt; Fiscal Year: 2006..abstract_text> ..
- An Integrated PET-MRI System for Molecular ImagingBernd Pichler; Fiscal Year: 2005..abstract_text> ..
- Carbon Nanotube Nanoelectrode Array/Deep Brain Stimulation & Electrochemical RecoJun Li; Fiscal Year: 2007..abstract_text> ..
- Oxygen microscopy by two-photon excited phosphorescenceSergei Vinogradov; Fiscal Year: 2010..The biological systems are selected to evaluate the performance of the technique and to simultaneously obtain valuable information for the related bio-medical problems. ..
- Genes, Hormones & Environment in an Ovarian Cancer ModelDaniel Cramer; Fiscal Year: 2007..Our continued goal is to identify modifiable risk factors and chemo preventive agents for a common and lethal cancer in women. ..
- Subcellular regulation of gamma delta TCR in DETCsTomasz Zal; Fiscal Year: 2007..Our Aim 2 is to locate the molecular determinants in the structures of CD3zeta and FcRgamma which regulate incorporation of each chain into the gdTCR complex in DETCs. ..
- 5-Day Fiber-Coupled Sensor Interstitial GlucoseRalph Ballerstadt; Fiscal Year: 2006..We believe the proposed sensor can provide a solution for short-term (3-5 days) continuous monitoring that will be accurate, reliable, and convenient for patient use. [unreadable] [unreadable] [unreadable]..
- Droplet-Based Digital Microfluidic Genome SequencingRICHARD FAIR; Fiscal Year: 2006..unreadable] [unreadable]..
- MODEL STUDIES OF LOW BARRIER HYDROGEN BONDS IN CATALYSISEric Anslyn; Fiscal Year: 2001..The significance of such a result goes beyond an increased basic science understanding of enzymology, and has clear ramifications in the design of synthetic catalysts for biomedical and synthetic applications. ..
- Fluorescence Sensor Probe for Transcutaneous Glucose SensingRalph Ballerstadt; Fiscal Year: 2008..The overall result will be a healthier life for people with diabetes and lower medical costs for diabetes care for the society. [unreadable] [unreadable] [unreadable]..
- Mouse Modeling Techniques for Bioluminescence TomographyWenxiang Cong; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..