microchemistry

Summary

Summary: The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.

Top Publications

  1. ncbi Microfabricated arrays of femtoliter chambers allow single molecule enzymology
    Yannick Rondelez
    Nat Biotechnol 23:361-5. 2005
  2. ncbi Microfluidics in structural biology: smaller, faster em leader better
    Carl Hansen
    Department of Applied Physics, California Institute of Technology, MS 128 95, Pasadena, CA 91125, USA
    Curr Opin Struct Biol 13:538-44. 2003
  3. ncbi An artificial taste sensor based on conducting polymers
    A Riul Júnior
    Embrapa Instrumentação Agropecuária, CP 741, CEP 13560 970 São Carlos, SP, Brazil
    Biosens Bioelectron 18:1365-9. 2003
  4. ncbi Physics and applications of microfluidics in biology
    David J Beebe
    Department of Biomedical Engineering, University of Wisconsin, Madison 53706, USA
    Annu Rev Biomed Eng 4:261-86. 2002
  5. ncbi Micropattern formation in supported lipid membranes
    Jay T Groves
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Acc Chem Res 35:149-57. 2002
  6. ncbi Cramming more sequencing reactions onto microreactor chips
    John H Leamon
    RainDance Technologies, Inc, 530 Whitfield Street, Guilford, Connecticut 06437, USA
    Chem Rev 107:3367-76. 2007
  7. ncbi Digital concentration readout of single enzyme molecules using femtoliter arrays and Poisson statistics
    David M Rissin
    Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, Massachusetts 02155, USA
    Nano Lett 6:520-3. 2006
  8. ncbi Disposable real-time microPCR device: lab-on-a-chip at a low cost
    Pavel Neuzil
    Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Nanos 04 01, Singapore 138669
    Mol Biosyst 2:292-8. 2006
  9. ncbi Micro total analysis systems. Latest advancements and trends
    Petra S Dittrich
    Institute for Analytical Sciences, Bunsen Kirchhoff Strasse 11, D 44139 Dortmund, Germany
    Anal Chem 78:3887-908. 2006
  10. ncbi Microfluidic technologies in clinical diagnostics
    Thomas H Schulte
    Micronics, Inc, Redmond, WA, USA
    Clin Chim Acta 321:1-10. 2002

Research Grants

  1. BIOMEDICAL RESEARCH SUPPORT
    HENRYK WISNIEWSKI; Fiscal Year: 1991
  2. GAD65 PEPTIDES BOUND TO IDDM-ASSOCIATED MHC PROTEINS
    JACK STROMINGER; Fiscal Year: 2003
  3. SALIVARY PROTEINS IN DENTAL INTEGUMENTS
    FRANK OPPENHEIM; Fiscal Year: 2000
  4. Rapid Production of PET Brain Ligands using Self Optimizing Microchemistry
    JOSEPH MATTEO; Fiscal Year: 2006
  5. PURCHASE OF LCQ MASS SPECTROMETER SYSTEM
    Joshua Hamilton; Fiscal Year: 2001
  6. NEW TECHNIQUE FOR PICOLITER INJECTION
    Harry Fein; Fiscal Year: 1992
  7. NEW TECHNIQUE FOR PICOLITER INJECTION
    Harry Fein; Fiscal Year: 1990
  8. MOLECULAR STUDIES USING AN AUTOMATED DNA SEQUENCER
    Minnie McMillan; Fiscal Year: 2005
  9. BECKMAN SYNCHRON CX5 DELTA CHEMISTRY ANALYSIS SYSTEM
    Edward Leiter; Fiscal Year: 1999
  10. PHEROMONES--IDENTIFICATION/BIOCHEMICAL STUDIES
    Milos Novotny; Fiscal Year: 2001

Detail Information

Publications237 found, 100 shown here

  1. ncbi Microfabricated arrays of femtoliter chambers allow single molecule enzymology
    Yannick Rondelez
    Nat Biotechnol 23:361-5. 2005
    ..The approach should be of interest for many ultrasensitive bioassays at the single-molecule level...
  2. ncbi Microfluidics in structural biology: smaller, faster em leader better
    Carl Hansen
    Department of Applied Physics, California Institute of Technology, MS 128 95, Pasadena, CA 91125, USA
    Curr Opin Struct Biol 13:538-44. 2003
    ..Diverse applications ranging from time-resolved studies of protein folding to highly efficient protein crystal growth suggest that microfluidics may become an indispensable tool in biology...
  3. ncbi An artificial taste sensor based on conducting polymers
    A Riul Júnior
    Embrapa Instrumentação Agropecuária, CP 741, CEP 13560 970 São Carlos, SP, Brazil
    Biosens Bioelectron 18:1365-9. 2003
    ..Brands of several commercial beverages could be easily distinguished without complex analysis, including the discrimination of waters, tastants and wines...
  4. ncbi Physics and applications of microfluidics in biology
    David J Beebe
    Department of Biomedical Engineering, University of Wisconsin, Madison 53706, USA
    Annu Rev Biomed Eng 4:261-86. 2002
    ..The focus of this review is microscale phenomena and the use of the physics of the scale to create devices and systems that provide functionality useful to the life sciences...
  5. ncbi Micropattern formation in supported lipid membranes
    Jay T Groves
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Acc Chem Res 35:149-57. 2002
    ..Collectively, these preparative and analytical patterned membrane techniques offer a broad experimental platform for the study and utilization of lipid membranes...
  6. ncbi Cramming more sequencing reactions onto microreactor chips
    John H Leamon
    RainDance Technologies, Inc, 530 Whitfield Street, Guilford, Connecticut 06437, USA
    Chem Rev 107:3367-76. 2007
  7. ncbi Digital concentration readout of single enzyme molecules using femtoliter arrays and Poisson statistics
    David M Rissin
    Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, Massachusetts 02155, USA
    Nano Lett 6:520-3. 2006
    ..This approach should prove useful for single molecule enzymology and ultrasensitive bioassays. More generally, the ability to determine concentration by counting individual molecules offers a new approach to analysis of dilute solutions...
  8. ncbi Disposable real-time microPCR device: lab-on-a-chip at a low cost
    Pavel Neuzil
    Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Nanos 04 01, Singapore 138669
    Mol Biosyst 2:292-8. 2006
    ..As the glass slip is disposable, cross-contamination from sample to sample is eliminated. The total cost of running the PCR is given by the value of the cover slip and its treatment...
  9. ncbi Micro total analysis systems. Latest advancements and trends
    Petra S Dittrich
    Institute for Analytical Sciences, Bunsen Kirchhoff Strasse 11, D 44139 Dortmund, Germany
    Anal Chem 78:3887-908. 2006
  10. ncbi Microfluidic technologies in clinical diagnostics
    Thomas H Schulte
    Micronics, Inc, Redmond, WA, USA
    Clin Chim Acta 321:1-10. 2002
    ..The laminar flow behavior of fluids in microfluidic devices must be accommodated in the design and development of clinical and bio-clinical miniaturized systems...
  11. ncbi Determination of carcinoembryonic antigen in human sera by integrated bead-bed immunoassay in a microchip for cancer diagnosis
    K Sato
    Department of Applied Chemistry, School of Engineering, The University of Tokyo, Hongo, Bunkyo, Japan
    Anal Chem 73:1213-8. 2001
    ..This microchip-based diagnosis system is the first microchip-based system that is practically useful for clinical diagnoses with short analysis time, high sensitivity, and easy procedures...
  12. ncbi Disposable immunosensor for the determination of domoic acid in shellfish
    L Micheli
    Dipartimento di Scienze e Tecnologie Chimiche, Universita Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
    Biosens Bioelectron 20:190-6. 2004
    ..Very good recoveries were obtained, demonstrating the suitability of the proposed assay for accurate determination of the DA concentration in mussel samples...
  13. ncbi Continuous flow immunosensor for highly selective and real-time detection of sub-ppb levels of 2-hydroxybiphenyl by using surface plasmon resonance imaging
    K Vengatajalabathy Gobi
    Art, Science and Technology Center for Cooperative Research, Kyushu University, Kasuga shi, Fukuoka 816 8580, Japan
    Biosens Bioelectron 20:350-7. 2004
    ..The SPR sensor for HBP is proved to be applicable in simultaneous detection of HBP and BaP in parallel with another SPR sensor for BaP...
  14. ncbi A portable automated system for trace gas sampling in the field and stable isotope analysis in the laboratory
    Daniel E Theis
    Paul Scherrer Institute, Laboratory of Atmospheric Chemistry, 5232 Villigen, Switzerland
    Rapid Commun Mass Spectrom 18:2106-12. 2004
    ..Our system should therefore further facilitate research of trace gases in the context of the carbon cycle in the field, and opens many other possible applications with carbon- and possibly non-carbon-containing trace gases...
  15. ncbi Experimental equivalent cluster-size distributions in nanometric volumes of liquid water
    B Grosswendt
    Physikalisch Technische Bundesanstalt, Bundesallee 100, D 38116 Braunschweig, Germany
    Radiat Prot Dosimetry 110:851-7. 2004
    ....
  16. ncbi Verification of performance with the automated direct optical TIRF immunosensor (River Analyser) in single and multi-analyte assays with real water samples
    Jens Tschmelak
    Institute of Physical and Theoretical Chemistry IPTC, Eberhard Karls University of Tuebingen, Auf der Morgenstelle 8, D 72076 Tuebingen, Germany
    Biosens Bioelectron 20:743-52. 2004
    ..Robustness could be demonstrated by long-term stability tests of the biosensor surface. These studies show that the biosensor used offers the necessary reproducibility, precision, and robustness required for an analytical method...
  17. ncbi In-situ quantitative analysis of a prostate-specific antigen (PSA) using a nanomechanical PZT cantilever
    Kyo Seon Hwang
    Microsystem Research Center, Korea Institute of Science and Technology, Seongbuk gu, Seoul 136 761, Republic of Korea
    Lab Chip 4:547-52. 2004
    ..The saturated resonance frequency shift of the cantilever was proportional to the PSA antigen concentration of analyte solution...
  18. ncbi High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems
    Sandro Cesaro-Tadic
    IBM Research GmbH, Zurich Research Laboratory, Saumerstrasse 4, CH 8803 Ruschlikon, Switzerland
    Lab Chip 4:563-9. 2004
    ..6 mm(2) on PDMS to detect TNF-alpha in 600 nL of a dendritic cell (DC) culture medium with a sensitivity of approximately 20 pg mL(-1)(1.14 pM)...
  19. ncbi Analysing a magnetic molecule detection system--computer simulation
    W Schepper
    Department of Physics, University of Bielefeld, Universitatsstrasse 25, 33501 Bielefeld, Germany
    J Biotechnol 112:35-46. 2004
    ..We can present first calculations where the bead particles (signal source) and the magnetic layers (sensor device) are considered as a whole magnetic ensemble...
  20. ncbi Development of an optical immunosensor based on the fluorescence of Cyanine-5 for veterinarian diagnostics
    Marta Silva
    Biosensors Laboratory, IBET ITQB Instituto de Biologia Experimental e Tecnológica Instituto de Tecnologia Química e Biológica, Apartado 12, P 2781 901 Oeiras, Portugal
    Biotechnol Lett 26:993-7. 2004
    ..Photobleaching had no effect on the fluorescence. This model system was used to detect Brucella sp. infection and could quantify anti-Brucella sp. antibodies in ovine serum samples in the range from 0.005 to 0.11 mg ml(-1)...
  21. ncbi An ultrasensitive and stable potentiometric immunosensor
    D Purvis
    Scientific Generics Ltd, Harston Mill, Harston, Cambridge CB2 5NH, UK
    Biosens Bioelectron 18:1385-90. 2003
    ..All these assays were performed using complex fluid samples and the presence of any non-specific binding has no significant effect on the final measurement. New assays can be transferred and optimised readily...
  22. ncbi Planar microcoil-based microfluidic NMR probes
    C Massin
    Institute of Microelectronics and Microsystems, EPFL Swiss Federal Institute of Technology Lausanne, BM 3 108, Lausanne CH 1015, Switzerland
    J Magn Reson 164:242-55. 2003
    ..Finally, based on the presented model and measured data, future performance of planar microcoil-based microfluidic NMR probes is extrapolated and discussed...
  23. ncbi A high-performance glucose biosensor based on monomolecular layer of glucose oxidase covalently immobilised on indium-tin oxide surface
    Aiping Fang
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
    Biosens Bioelectron 19:43-9. 2003
    ..These characteristics make it possible to achieve mass production of biosensor compatible with the current electronic integrated circuit manufacturing technologies...
  24. ncbi Nucleic acid purification using microfabricated silicon structures
    Nathaniel C Cady
    Department of Microbiology, Cornell University, 317 Stocking Hall, Ithaca, NY 14853, USA
    Biosens Bioelectron 19:59-66. 2003
    ....
  25. ncbi Optical-transparent and flexible glucose sensor with ITO electrode
    Kohji Mitsubayashi
    Department of Human and Information Science, School of Engineering, Tokai University, 1117 Kitakaname, Hiratsuka, Kanagawa 259 1292, Japan
    Biosens Bioelectron 19:67-71. 2003
    ..The sensor was possible to be used for measuring glucose from 0.06 to 1.24 mmol/l (correlation coefficient: 0.999), including the reported concentration of tear glucose in normal (0.14 mmol/l), with good reproducibility...
  26. ncbi Development of a conductimetric biosensor using immobilised Rhodococcus ruber whole cells for the detection and quantification of acrylonitrile
    P C J Roach
    Biotechnology Centre, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, West Yorkshire, HD1 3DH, UK
    Biosens Bioelectron 19:73-8. 2003
    ..The biosensor has been shown to be reproducible with respect to the data obtained over a number of days, and retains stability for a minimum period of at least 5 days before recalibration of the biosensor is required...
  27. ncbi A micromachined capillary electrophoresis chip with fully integrated electrodes for separation and electrochemical detection
    Ralph Wilke
    Institute for Microtechnology, Technical University of Braunschweig, Alte Salzdahlumer Str 203, 38124 Braunschweig, Germany
    Biosens Bioelectron 19:149-53. 2003
    ..All three oxidizable species could be detected in less than 70 s. Glucose was detected by performing an enzymatic reaction along the separation channel. The microsystem showed a very good reproducibility...
  28. ncbi Redox labelled avidin for enzyme sensor architectures
    Celestino Padeste
    Laboratory for Micro and Nanotechnology, Paul Scherrer Institut, CH 5232 Villigen PSI, Switzerland
    Biosens Bioelectron 19:239-47. 2003
    ..1 mM, respectively. The molecular architecture concept allows quick adaptation of the sensors to other analytes, and it provides a platform for arrays of sensors with different selectivity...
  29. ncbi Simultaneous determination of L- and D-methotrexate using a sequential injection analysis/amperometric biosensors system
    Raluca Ioana Stefan
    Department of Chemistry, University of Pretoria, Pretoria 0002, South Africa
    Biosens Bioelectron 19:261-7. 2003
    ..The SIA/biosensors system can be used reliably on-line in synthesis process control, for the simultaneous assay of L- and D-Mtx with a frequency of 34 samples per hour...
  30. ncbi Detection of heavy metal ions using protein-functionalized microcantilever sensors
    Suman Cherian
    Life Sciences Division, Oak Ridge National Laboratory, Building 4500S, Oak Ridge, TN 37831 6123, USA
    Biosens Bioelectron 19:411-6. 2003
    ..Thus, we demonstrate that microcantilever bending can be used to discriminate between metal ions that bind and do not bind to AgNt84-6 protein in real time...
  31. ncbi Nanoporous aluminum oxide as a novel support material for enzyme biosensors
    A Heilmann
    Fraunhofer Institute for Mechanics of Materials, D 06120 Halle, Saale, Germany
    J Nanosci Nanotechnol 3:375-9. 2003
    ..The achieved operational stability showed that alumina membranes can be used to construct enzyme-modified electrodes...
  32. ncbi Electrocatalytic reduction of chloramphenicol at multiwall carbon nanotube-modified electrodes
    Shaofang Lu
    Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, China
    J Nanosci Nanotechnol 3:401-5. 2003
    ..Interfering experiments show that the modified electrode has excellent selectivity for the detection of CAP. The modified electrode was used to determine CAP in eyedrops, and the recoveries were approximately 100%...
  33. ncbi Integrated optical-fiber capillary electrophoresis microchips with novel spin-on-glass surface modification
    Che Hsin Lin
    Department of Mechanical and Electro Mechanical Engineering, National Sun Yat Sen University, Taiwan, ROC
    Biosens Bioelectron 20:83-90. 2004
    ....
  34. ncbi Nano- and pico-dispensing of fluids on planar substrates using SAW
    Christoph J Strobl
    Department für Physik and Center for NanoScience CeNS, Geschwister Scholl Platz 1, University of Munich, D 80539 Munich, Germany
    IEEE Trans Ultrason Ferroelectr Freq Control 51:1432-6. 2004
    ..In addition to a detailed description of the technique, several examples of applications are presented...
  35. ncbi Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes
    Yasutaka Matsubara
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1 1 Asahidai, Tatsunokuchi, Nomi gun, Ishikawa 923 1292, Japan
    Biosens Bioelectron 20:1482-90. 2005
    ..Such a small device is surely a strong candidate for low-cost DNA amplification, and detected as little as 0.4 copies of target DNA...
  36. ncbi Rapid, femtomolar bioassays in complex matrices combining microfluidics and magnetoelectronics
    S P Mulvaney
    Naval Research Laboratory, Washington, DC 20375, USA
    Biosens Bioelectron 23:191-200. 2007
    ....
  37. pmc Microfluidic vias enable nested bioarrays and autoregulatory devices in Newtonian fluids
    Emil P Kartalov
    Department of Biochemistry and Molecular Biology, Keck School of Medicine and Norris Cancer Center, University of Southern California, NOR6346, 1441 Eastlake Avenue, Los Angeles, CA 90033, USA
    Proc Natl Acad Sci U S A 103:12280-4. 2006
    ..We also describe microfluidic septa and their applications. Vias lay the foundation for a new generation of microfluidic devices...
  38. ncbi Nano-dispensing by electrospray for biotechnology
    Maxim E Kuil
    Biophysical Structure Chemistry, Leiden Institute for Chemistry, Leiden, The Netherlands
    Biotechnol J 1:969-75. 2006
    ..Experimental results and the design of a novel spraying configuration, which can be incorporated in an optical microscope, are reported...
  39. ncbi Single-ion dosemeter based on floating gate memories
    G Cellere
    Department of Information Engineering, Padova University, Padova, Italy
    Radiat Prot Dosimetry 122:457-9. 2006
    ..This paper discusses the opportunities to use this devices as single an ion dosemeter with sub-micrometer spatial resolution and capable of distinguish the impinging ion LET...
  40. pmc On-chip acidification rate measurements from single cardiac cells confined in sub-nanoliter volumes
    Igor A Ges
    Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA
    Biomed Microdevices 10:347-54. 2008
    ..8 mM of Ca(2+). The NanoPhysiometer concept can be extended to study ischemia/reperfusion injury or disorders of other biological systems to identify strategies for treatment and possible pharmacological targets...
  41. ncbi PCB/polymer based micro-fluidic system for NMR spectroscopy for nanoliters sample volume
    Guillaume Pasquet
    Institut des Nanotechnologies de Lyon INL, University Claude Bernard, Villeurbanne, 69622 France
    Conf Proc IEEE Eng Med Biol Soc 2007:2968-71. 2007
    ..The fabrication process is described, and first results are reported. The tight sealing of the micro-fluidic system has been demonstrated and preliminary NMR experiment results are presented...
  42. ncbi Electromechanical transducers at the nanoscale: actuation and sensing of motion in nanoelectromechanical systems (NEMS)
    K L Ekinci
    Department of Aerospace and Mechanical Engineering, Boston University, Boston, MA 02215, USA
    Small 1:786-97. 2005
    ..The currently available techniques to actuate and detect NEMS motion are introduced, and the accuracy, bandwidth, and robustness of these techniques are discussed...
  43. ncbi On-chip oligonucleotide ligation assay using one-dimensional microfluidic beads array for the detection of low-abundant DNA point mutations
    He Zhang
    State Key Laboratory of Chemo Biosensing and Chemometrics, Biomedical Engineering Center, College of Chemistry and Chemical Engineering, Key Laboratory for Bio Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, PR China
    Biosens Bioelectron 23:945-51. 2008
    ..Additionally, this on-chip beads ligation approach also displayed other microfluidic-based advantages of simple handling (one sample injection per test), little reagent quantities, and low potential of contaminations...
  44. ncbi Temperature distribution effects on micro-CFPCR performance
    Pin Chuan Chen
    Center for Bio Modular Multi Scale Systems, Louisiana State University, Baton Rouge, LA 70803, USA
    Biomed Microdevices 10:141-52. 2008
    ..Lab Chip 4:638, 2004) strictly due to the improved temperature distribution...
  45. ncbi Micropumps, microvalves, and micromixers within PCR microfluidic chips: Advances and trends
    Chunsun Zhang
    MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, No 55, Zhongshan Avenue West, Tianhe District, Guangzhou 510631, PR China
    Biotechnol Adv 25:483-514. 2007
    ..Finally, general discussions on microvalves, micropumps, and micromixers for PCR chips are given. The microvalve/micropump/micromixers allow high levels of PCR chip integration and analytical throughput...
  46. ncbi A suspended membrane nanocalorimeter for ultralow volume bioanalysis
    Erik A Johannessen
    Department of Human Anatomy and Cell Biology, University of Liverpool, New Medical School, L69 3LT Liverpool, UK
    IEEE Trans Nanobioscience 1:29-36. 2002
    ..The nanocalorimetric sensor has the potential for integration in an ultralow-volume high-density array format for the characterization of processes in which there is an exchange of heat...
  47. ncbi Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods
    A V Nabok
    Sheffield Hallam University, Materials and Engineering Research Institute, City Campus, Pond Street, Sheffield S1 1WB, UK
    Biosens Bioelectron 22:885-90. 2007
    ..The QCM impedance measurements data showed anomalously large mass increase and film softening, most likely, due to the binding of large T-2 aggregates to antibodies...
  48. ncbi Automated Water Analyser Computer Supported System (AWACSS) Part II: Intelligent, remote-controlled, cost-effective, on-line, water-monitoring measurement system
    Jens Tschmelak
    Institute of Physical and Theoretical Chemistry IPTC, Eberhard Karls University of Tuebingen, Auf der Morgenstelle 8, Tuebingen 72076, Germany
    Biosens Bioelectron 20:1509-19. 2005
    ....
  49. ncbi Electrochemical determination of reversible redox species at interdigitated array micro/nanoelectrodes using charge injection method
    Xiaoshan Zhu
    Department of Electrical and Computer Engineering and Computer Science, University of Cincinnati, Cincinnati, OH 45221 0030, USA
    IEEE Trans Nanobioscience 4:164-9. 2005
    ..Using p-aminophenol as the redox species, approximately 4 x 10(-7) M and 6 x 10(-9) M detection limits on the species concentration ar respectively achieved with the microelectrodes and the nanoelectrodes...
  50. ncbi Catheter-tip sensor to monitor production of hydrogen peroxide in small biosamples
    Chaitanya S Sathe
    Applied Biology and Biomedical Engineering, Rose Hulman Institute of Technology, Terre Haute, Indiana 47803, USA
    Biomed Sci Instrum 41:193-8. 2005
    ..7 microM rat IgG solution. We further discuss the usefulness, limitation and the future scope of this real time monitoring system for H2O2 research using small biosamples...
  51. ncbi Precise nanoliter fluid handling system with integrated high-speed flow sensor
    Carsten Haber
    Seyonic SA, Neuchatel, Switzerland
    Assay Drug Dev Technol 3:203-12. 2005
    ..The collected dispense information can be used for quality control assessments and automatically be made part of an electronic record...
  52. ncbi Nanoliter dispensing for uHTS using pin tools
    Patrick H Cleveland
    V and P Scientific, Inc, San Diego, CA 92121, USA
    Assay Drug Dev Technol 3:213-25. 2005
    ..The factors that affect liquid transfers by pin tools are discussed in detail as well as the advantages and limitations of pin tools...
  53. ncbi Amperometric biosensor based on tyrosinase-conjugated polysaccharide hybrid film: selective determination of nanomolar neurotransmitters metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC) in biological fluid
    Aihua Liu
    Energy Technology Research Institute, National Institute of Advanced Industrial Science and Technology AIST, Umezono 1 1 1, Tsukuba 305 8568, Japan
    Biosens Bioelectron 21:809-16. 2005
    ..A physiological level of neurotransmitters and their derivatives including dopamine, l-dopa, adrenaline, noradrenaline and homovanillic acid as well as ascorbic acid, uric acid and acetaminophen do not affect the determination of DOPAC...
  54. ncbi Micro gas analysis system for measurement of atmospheric hydrogen sulfide and sulfur dioxide
    Shin ichi Ohira
    Department of Science, Kumamoto University, 2 39 1, Kurokami, Kumamoto, 860 8555, Japan
    Lab Chip 5:1374-9. 2005
    ..The system we developed allowed automated continuous analyses in the field and achieved a much higher time resolution compared to those by ion chromatographic analysis...
  55. ncbi Cantilever sensor for nanomechanical detection of specific protein conformations
    Rupa Mukhopadhyay
    Interdisciplinary Nanoscience Center, Department of Physics and Astronomy, University of Aarhus, 8000 Aarhus C, Denmark
    Nano Lett 5:2385-8. 2005
    ....
  56. ncbi Integrated potentiostat for neurotransmitter sensing. A high sensitivity, wide range VLSI design and chip
    Kartikeya Murari
    Johns Hopkins School of Medicine, Baltimore, Maryland, USA
    IEEE Eng Med Biol Mag 24:23-9. 2005
  57. ncbi Electrohydrodynamic (EHD) dispensing of nanoliter DNA droplets for microarrays
    Jeong Gun Lee
    Samsung Advanced Institute of Technology, Mt 14 1, Nongseo ri, Giheung eup, Yongin si, Gyeonggi Do, Korea
    Biosens Bioelectron 21:2240-7. 2006
    ..Consequently, it is found that EHD dispensing method can be used to make cost-effective DNA microarrays with no thermal and electrical influences on DNA properties...
  58. ncbi Miniature sensor suitable for electronic nose applications
    Lal A Pinnaduwage
    Oak Ridge National Laboratory, P O Box 2008, Oak Ridge, TN 37831 6122, USA
    Rev Sci Instrum 78:055101. 2007
    ..The sensor can detect parts-per-trillion concentrations of DMMP within 10 s exposure times. The small size of the sensor makes it ideally suited for electronic nose applications...
  59. ncbi Single gallium nitride nanowire lasers
    Justin C Johnson
    Department of Chemistry, University of California, Berkeley, California 94720 1460, USA
    Nat Mater 1:106-10. 2002
    ..This study is a considerable advance towards the realization of electron-injected, nanowire-based ultraviolet-blue coherent light sources...
  60. pmc Probing distance and electrical potential within a protein pore with tethered DNA
    Stefan Howorka
    Department of Medical Biochemistry and Genetics, The Texas A and M University System Health Science Center, 440 Reynolds Medical Building, College Station, TX 77843 1114, USA
    Biophys J 83:3202-10. 2002
    ....
  61. ncbi Design and fabrication of a silica on silicon integrated optical biochip as a fluorescence microarray platform
    Jesus M Ruano
    Department of Electronics and Electrical Engineering, The University of Glasgow, Glasgow G12 8LT, UK
    Biosens Bioelectron 18:175-84. 2003
    ..Finally, in this paper, we describe the implementation of a pumping system for delivering small amounts of fluid across the array together with an optical signal treatment...
  62. ncbi Optical biosensor for urea with improved response time
    Barna Kovacs
    Department for General and Physical Chemistry, University of Pecs, 7624 Pecs, Ifjúság u 6, Hungary
    Biosens Bioelectron 18:111-8. 2003
    ..Thirty sample/hour analysis-rate, good peak-to-peak reproducibility (RSD=0.02) and recovery (95-104%) was achieved with buffer diluted urea solutions. Applications for the analysis of real samples are planned to do in the future...
  63. ncbi High sensitivity detection of molecular recognition using magnetically labelled biomolecules and magnetoresistive sensors
    D L Graham
    INESC Microsystems and Nanotechnologies, R Alves Redol 9, 1000 Lisbon, Portugal
    Biosens Bioelectron 18:483-8. 2003
    ..A wide range of applications is foreseen for this system in the development of biochips, high sensitivity biosensors and the detection of single molecules and single molecule interactions...
  64. ncbi Conventional detection of 2,4-dinitrophenol using quartz crystal microbalance
    Jong Won Park
    IEEE Trans Ultrason Ferroelectr Freq Control 50:193-5. 2003
    ..This QCM method allows detection of DNP concentration in the range of 0.01 to 100 ng/ml; linear correlation obtains DNP concentration from 1 to 100 ng/ml...
  65. ncbi Development and study of an amperometric biosensor for the in vitro measurement of low concentration of putrescine in blood
    Livia Nagy
    Hungarian Academy of Sciences, Research Group for Chemical Sensors, Ifjúság u 6, 7624 Pecs, Hungary
    J Biochem Biophys Methods 53:165-75. 2002
    ..A detection limit of 50 nM was achieved in pH-adjusted whole blood samples, which is below pathological levels...
  66. ncbi Fabrication and evaluation of a carbon-based dual-electrode detector for poly(dimethylsiloxane) electrophoresis chips
    A J Gawron
    Department of Pharmaceutical Chemistry and Center for Bioanalytical Research, University of Kansas, Lawrence 66047, USA
    Electrophoresis 22:242-8. 2001
    ..These devices illustrate the potential for a rugged and easily constructed microchip CE system with an integrated carbon-based detector of similar scale...
  67. ncbi Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection
    Ibolya Bontidean
    Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P O Box 124, SE 221 00, Lund, Sweden
    Biosens Bioelectron 18:547-53. 2003
    ..The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days...
  68. ncbi A fractal analysis of protein to DNA binding kinetics using biosensors
    Ajit Sadana
    Chemical Engineering Department, University of Mississippi, Post Office Box 1848, University, MS 38677 1848, USA
    Biosens Bioelectron 18:985-97. 2003
    ..Even though these reactions are occurring on the biosensor surface, the relationships presented should assist in understanding and in possibly manipulating the reactions occurring on cellular surfaces...
  69. ncbi An amperometric bi-enzyme sensor for determination of formate using cofactor regeneration
    Karen K W Mak
    Sino German Nano Analytical Lab SiGNAL and Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
    Biosens Bioelectron 18:1095-100. 2003
    ..98 x 10(-7) M for formate (S/N=3), with the response time of 4 min. The working stability is limited to 7 days due to the inactivation of the enzymes. Only sodium salicylate was needed in milli-molar amounts...
  70. pmc The shape parameter of liposomes and DNA-lipid complexes determined by viscometry utilizing small sample volumes
    Y Sun
    Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, China
    Biophys J 85:1223-32. 2003
    ..7 as prolate ellipsoids or 4.9 as oblate ellipsoids. The deviation of shape from perfect sphere is thus expressed quantitatively in more than two significant figures. In these measurement, the necessary amount of DNA is <0.5 mg...
  71. ncbi Highly sensitive biosensing using a supercritical angle fluorescence (SAF) instrument
    Thomas Ruckstuhl
    Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057, Zurich, Switzerland
    Biosens Bioelectron 18:1193-9. 2003
    ..The performance of the system is demonstrated by real time measurements of antibody-antigen reactions. Rate constants of the reaction were extracted. Antigen concentrations were detected down to 10(-13) mol/l...
  72. ncbi In situ TEM of radiation effects in complex ceramics
    Jie Lian
    Department of Mechanical, Aerospace and Nuclear Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
    Microsc Res Tech 72:165-81. 2009
    ..irradiations have been summarized with a focus on irradiation-induced microstructural evolution, changes in microchemistry, and the formation of nanostructures...
  73. ncbi Two new male contraceptives exert their effects by depleting germ cells prematurely from the testis
    C Y Cheng
    Population Council, The Rockefeller University, New York, New York 10021, USA
    Biol Reprod 65:449-61. 2001
    ..In addition, results of serum microchemistry illustrate that liver and kidney function was not affected in animals treated with both compounds.
  74. ncbi Critical care medicine: opportunities and strategies for improvement
    I L Cohen
    Department of Surgery, Buffalo General Hospital, Buffalo, New York 14203, USA
    Jt Comm J Qual Improv 22:85-103. 1996
    ..Like other areas of health care, critical care faces increasing pressure to improve the quality while reducing the cost of care. Strategies drawn from the literature and the authors' experiences are presented...
  75. pmc Distinct migratory and non-migratory ecotypes of an endemic New Zealand eleotrid (Gobiomorphus cotidianus) - implications for incipient speciation in island freshwater fish species
    Christian Michel
    Limnological Institute, University of Konstanz, Konstanz, Germany
    BMC Evol Biol 8:49. 2008
    ....
  76. ncbi Alkali element uptake in otoliths: a link between the environment and otolith microchemistry
    Lisa A Friedrich
    Department of Geological Sciences, University of Manitoba, Winnipeg MB, Canada R3T 2N2
    Environ Sci Technol 42:3514-8. 2008
    ..between the elements in the otoliths and surrounding rocks suggests there can be a strong link between the microchemistry of a fish's otoliths and its environment particularly where there are distinctive rock types...
  77. ncbi Larval quality is shaped by matrix effects: implications for connectivity in a marine metapopulation
    Jeffrey S Shima
    School of Biological Sciences, Coastal Ecology Lab, P O Box 600, Victoria University of Wellington, Wellington 6140, New Zealand
    Ecology 90:1255-67. 2009
    ..We used laser ablation-inductively coupled plasma-mass spectrometry to quantify otolith microchemistry, followed by a set of cluster analyses (based upon 13 statistical descriptors of time series for each of 11 ..
  78. ncbi The legacy of dispersal: larval experience shapes persistence later in the life of a reef fish
    Jeffrey S Shima
    School of Biological Sciences, PO Box 600, Victoria University of Wellington, New Zealand Department of Zoology, University of Melbourne, VIC 3010 Australia
    J Anim Ecol 79:1308-14. 2010
    ..2. We evaluated such 'legacy effects' for dispersing reef fish larvae using (i) otolith (ear stone) microchemistry to characterize two distinct dispersal pathways and (ii) otolith microstructure to estimate 'larval quality' (..
  79. ncbi Laser surface treatment of hydroxyapatite for enhanced tissue integration: surface characterization and osteoblastic interaction studies
    S Teixeira
    Laboratorio de Biomateriais, INEB Instituto de Engenharia Biomedica, Rua do Campo Alegre 823, 4150 180, Porto, Portugal
    J Biomed Mater Res A 81:920-9. 2007
    Biocompatibility has long been associated with surface microtopography, microtexture, and microchemistry. The surface topography eventually affects the nature and the intensity of the interactions that occur at biomaterial-biological ..
  80. ncbi Interactions between MC3T3-E1 cells and textured Ti6Al4V surfaces
    W O Soboyejo
    Princeton Materials Institute, Princeton University, Princeton, New Jersey 08544, USA
    J Biomed Mater Res 62:56-72. 2002
    ..The potential cytotoxic effects of microchemistry on cell-surface interactions also are considered in studies of cell spreading and orientation over 9-day ..
  81. ncbi Chemical composition of saccular endolymph and otolith in fish inner ear: lack of spatial uniformity
    P Payan
    Laboratoire de Physiologie et Toxicologie Environnementales, EA 2138, France
    Am J Physiol 277:R123-31. 1999
    ..Thus the lack of uniformity in the otolith composition must be taken into account when investigating otolith microchemistry.
  82. ncbi Biomaterials, biomechanics, tissue healing, and immediate-function dental implants
    Jack E Lemons
    Department of Prosthodontics and Biomaterials, University of Alabama at Birmingham, SDB Box 49, Birmingham, AL 35294 0007, USA
    J Oral Implantol 30:318-24. 2004
    ..finn, and porous geometry endosteal dental-implant designs with and without alterations in device body-surface microchemistry and microtopography...
  83. ncbi A multi-locus assessment of connectivity and historical demography in the bluehead wrasse (Thalassoma bifasciatum)
    R A Haney
    Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, USA
    Heredity (Edinb) 98:294-302. 2007
    ..previous studies have found both substantial levels of local recruitment, in studies interpreting otolith microchemistry and, conversely, a lack of genetic differentiation inferred from mitochondrial DNA (mtDNA) restriction-..
  84. ncbi High-precision continuous-flow isotope ratio mass spectrometry
    J T Brenna
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Mass Spectrom Rev 16:227-58. 1997
    ..Capillary gas chromatography interfaced to IRMS via on-line microchemistry facilitates compound-specific isotope analysis (CSIA) for purified organic analytes of 1 nmol of C, N, or O...
  85. ncbi Photoemission and free electron laser spectromicroscopy: photoemission at high lateral resolution
    G Margaritondo
    Institut de Physique Appliquee, Ecole Polytechnique Federale, Lausanne, Switzerland
    Scanning Microsc 9:949-63; discussion 963. 1995
    ..physics and biology, such as local chemical fluctuations in superconductors, semiconductor interfaces and the microchemistry of biological systems...
  86. ncbi Towards inexpensive DNA diagnostics
    S Abramowitz
    National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Trends Biotechnol 14:397-401. 1996
    ..This will require the efficient integration of technical advances in microchemistry, micromachining, separation technologies, detection systems, microelectronics and information technology, and ..
  87. ncbi Thermooptical detection in microchips: from macro- to micro-scale with enhanced analytical parameters
    Adelina Smirnova
    Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo, Japan
    Electrophoresis 29:2741-53. 2008
    ..TLM showed low LODs in solvent extraction (down to 10(-8) M) and electrokinetic separation (10(-7) M), which were shown to be governed by discussed instrumental regularities, instead of by microchemistry.
  88. ncbi DEP actuated nanoliter droplet dispensing using feedback control
    Kai Liang Wang
    University of Rochester, Rochester, NY 14627, USA
    Lab Chip 9:901-9. 2009
    ..Reconfiguring the module connections and reprogramming the control module permits testing of a variety of control strategies...
  89. ncbi Capillary circular dichroism
    Daniel E Waldron
    Department of Chemistry, University of Warwick, Coventry, United Kingdom
    Chirality 22:E136-41. 2010
    ..The system performance was validated with Na[Co(EDDS)].H(2)O (EDDS = N,N-ethylenediaminedisuccinic acid), concanavalin A, lysozyme, and progesterone...
  90. ncbi Microfabricated device for DNA and RNA amplification by continuous-flow polymerase chain reaction and reverse transcription-polymerase chain reaction with cycle number selection
    Pierre J Obeid
    Department of Chemistry, University of Patras, Patras, Greece GR 26500
    Anal Chem 75:288-95. 2003
    ..To our knowledge, this is the first report of a monolithic microdevice that integrates continuous-flow RT and PCR with cycle number selection...
  91. ncbi Velocity measurement of particles flowing in a microfluidic chip using Shah convolution Fourier transform detection
    Y C Kwok
    AstraZeneca/SmithKline Beecham Centre for Analytical Sciences, Department of Chemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, United Kingdom
    Anal Chem 73:1748-53. 2001
    ..This technique could have wide applications, ranging from the determination of the velocity of particles in pressure-driven flow to the measurement of electrophoretic mobilities of single biological cells...
  92. ncbi Determination of trace metals in drinking water using solid-phase extraction disks and X-ray fluorescence spectrometry
    Xiandeng Hou
    Department of Chemistry, Wake Forest University, Winston Salem, North Carolina 27109, USA
    Appl Spectrosc 57:338-42. 2003
    ..Thus, the overall cost for analysis of metals in drinking water can be minimized by implementing the method, and small water supply companies with limited budgets will be better able to comply with the Safe Drinking Water Act...
  93. ncbi Identification card and codification of the chemical and morphological characteristics of 14 dental implant surfaces
    David M Dohan Ehrenfest
    LoB5 unit, Chonnam National University School of Dentistry, Gwangju, South Korea
    J Oral Implantol 37:525-42. 2011
    ..The DEC system could be an interesting basis for the development of a clear and simple ISO standard for dental implant surfaces and other implantable devices...
  94. ncbi A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative
    J G Mohanty
    MCP Hahnemann School of Medicine, Division of Pulmonary and Critical Care Medicine, Allegheny University of the Health Sciences, Philadelphia, PA 19102, USA
    J Immunol Methods 202:133-41. 1997
    ..The protocol described in this study using A6550 to measure H2O2 release from activated granulocytes can be easily adapted to other cell types which generate H2O2...
  95. ncbi Preparation and application of graphene-poly (diallyldimethylammoniumchloride)-iron oxide nanoparticles buckypaper for hydrogen peroxide detection
    Xueque You
    School of Electrical Engineering, Korea University, 5 ga, Anam Dong, Seongbuk gu, Seoul 136 713, Korea
    J Nanosci Nanotechnol 13:7349-57. 2013
    ..986, and a high sensitivity of 218 microA mM(-1) x cm(-2). Such low-cost G-PDDA-NP composite BPs prepared by facile methods pave way towards novel sensors with better performance...
  96. ncbi MEMS technology in analytical chemistry
    Anil K Deisingh
    Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4M1
    Analyst 128:9-11. 2003
    ..Anil Deisingh talks us through the basics of MEMS technology and the growing role of these new "micromachines" in analytical chemistry applications...
  97. ncbi Design and applications of sensitive enzyme immunoassays specific for clostridial enoate reductases
    G Krause
    , , Bundesrepublik Deutschland
    Z Naturforsch C 44:345-52. 1989
    ..thermoaceticum to disintegrate into its subunits and fragments as compared to its mesophilic counterpart...
  98. ncbi Microrheology of the liquid-solid transition during gelation
    Travis H Larsen
    Department of Chemical Engineering and Center for Molecular and Engineering Thermodynamics, University of Delaware, Newark, Delaware 19716, USA
    Phys Rev Lett 100:146001. 2008
    ..Both systems exhibit a critical relaxation exponent n approximately 0.6, where G' approximately G'' approximately omega n for the incipient gel, consistent with the Rouse model of dynamic scaling in the percolation universality class...
  99. ncbi Point-of-care testing
    M R Fiallos
    Division of Pediatrics, Department of Critical Care Medicine, University Community Hospital, Tampa, Florida, USA
    Pediatr Clin North Am 48:589-99. 2001
    ..The constant evolution and developments in [figure: see text] microchemistry and computer technology will make this area a dynamic part of medicine with the constant emergence of improved ..
  100. ncbi Rapid screening of the aglycone specificity of glycosidases: applications to enzymatic synthesis of oligosaccharides
    J E Blanchard
    Protein Engineering Network of Centres of Excellence of Canada and the Department of Chemistry, University of British Columbia, V6T 1Z1, Vancouver, BC, Canada
    Chem Biol 8:627-33. 2001
    ..CONCLUSIONS: The aglycone specificity of a glycosidase can be rapidly evaluated and requires only an appropriate fluorosugar inactivator, a substrate for assay of activity and a library of compounds for screening...
  101. pmc A modular microfluidic architecture for integrated biochemical analysis
    Kashan A Shaikh
    Department of Electrical and Computer Engineering, Micro and Nanotechnology Laboratory, and Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 102:9745-50. 2005
    ..In the second case, we used a DNAzyme-based biosensor to identify the presence of Pb(2+) (lead) at a sensitivity of 500 nM in <1 nl of solution...

Research Grants60

  1. BIOMEDICAL RESEARCH SUPPORT
    HENRYK WISNIEWSKI; Fiscal Year: 1991
    ..state-of-the- art instruments that are required additions to an existing, functional, and successful Protein Microchemistry Facility that is currently heavily utilized...
  2. GAD65 PEPTIDES BOUND TO IDDM-ASSOCIATED MHC PROTEINS
    JACK STROMINGER; Fiscal Year: 2003
    ..The peptides extracted from the HLA proteins will be analyzed in the Harvard Microchemistry Facility using several techniques...
  3. SALIVARY PROTEINS IN DENTAL INTEGUMENTS
    FRANK OPPENHEIM; Fiscal Year: 2000
    ..A multifaceted strategy employing protein microchemistry, immunology and recombinant DNA technology has been incorporated in this proposal in order to determine the ..
  4. Rapid Production of PET Brain Ligands using Self Optimizing Microchemistry
    JOSEPH MATTEO; Fiscal Year: 2006
    ..Phase II will also demonstrate efficacy of the compound using MicroPET at the Center for Molecular and Genomic Imaging at UC Davis. [unreadable] [unreadable] [unreadable]..
  5. PURCHASE OF LCQ MASS SPECTROMETER SYSTEM
    Joshua Hamilton; Fiscal Year: 2001
    ..This facility now offers protein microchemistry, protein sequencing, DNA sequencing and DNA synthesis to 75 laboratories...
  6. NEW TECHNIQUE FOR PICOLITER INJECTION
    Harry Fein; Fiscal Year: 1992
    ..and useful instrument with which to perform pneumatic microinjection of fluids in the life sciences and microchemistry. Experimental studies performed by the applicant in Phase I of this project confirm that pneumatic injection ..
  7. NEW TECHNIQUE FOR PICOLITER INJECTION
    Harry Fein; Fiscal Year: 1990
    ..and useful instrument with which to perform pneumatic microinjection of fluids in the life sciences and microchemistry. Studies will be performed to validate experimental results obtained by the applicant which indicate the ..
  8. MOLECULAR STUDIES USING AN AUTOMATED DNA SEQUENCER
    Minnie McMillan; Fiscal Year: 2005
    ..It would be housed in the USC/Norris Comprehensive Cancer Center Microchemistry Core Laboratory, which offers a DNA sequencing service to peer-reviewed investigators...
  9. BECKMAN SYNCHRON CX5 DELTA CHEMISTRY ANALYSIS SYSTEM
    Edward Leiter; Fiscal Year: 1999
    ..Funds are requested to purchase the Beckman Synchron Cx5 System, to be operated by the institution's Microchemistry Service on a fee for service basis thus providing a cost-effective way to fill an urgent, and currently unmet ..
  10. PHEROMONES--IDENTIFICATION/BIOCHEMICAL STUDIES
    Milos Novotny; Fiscal Year: 2001
    ..Understanding the aspects of chemical communication in the species could also be important in a population control of wild rodents. ..
  11. 2007 Microfluidics, Physics & Chemistry of Gordon Research Conference
    SABETH VERPOORTE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  12. Integrated Nanofluidic BioMEMS for Biomedical Diagnostics and Analysis
    Emil Kartalov; Fiscal Year: 2008
    ..abstract_text> ..
  13. Optical Tweezer Arrays Based on Optical Imaging Fibers
    DAVID WALT; Fiscal Year: 2007
    ..The technology has applicability to cell selection, blood and tumor diagnosis, combinatorial screening, nanoassembly, gene expression analysis and a wide variety of screening, selection, and diagnostic applications. ..
  14. Gas phase derivatization for lipidomic MS analysis
    JAMES BRENNA; Fiscal Year: 2007
    ....
  15. LCPUFA Status and Birth Outcome in India
    JAMES BRENNA; Fiscal Year: 2007
    ..Positive results would justify an intervention studies to include LCPUFA in the diets of pregnant women in developed and developing countries. ..
  16. Kinome Drug Bioassay Platform
    Haiching Ma; Fiscal Year: 2007
    ..Rapid HTS implementation for proprietary customer kinases using small amounts of purified protein is also a significant opportunity. [unreadable] [unreadable] [unreadable]..
  17. Development of SPR/MS protein array platform
    Dobrin Nedelkov; Fiscal Year: 2007
    ..abstract_text> ..
  18. Porous Biomimetric Nanocontainers
    Taekjip Ha; Fiscal Year: 2007
    ..Furthermore we will use these techniques to make new biological discoveries on the activities of RNA enzymes and helicases. ..
  19. Lipidomics Session ISSFAL 2006
    JAMES BRENNA; Fiscal Year: 2006
    ..ISSFAL 2006 is expected to be among the most important international lipid metabolism conferences of the year, and a lipidomics symposium is timely given the increasing progress in this area. [unreadable] [unreadable] [unreadable]..
  20. A mu Migration Assay for Temporal Protein Localization
    Maribel Vazquez; Fiscal Year: 2006
    ..Such ground-breaking results will elucidate unprecedented details of intracellular protein behavior during tumor cell dispersal. [unreadable] [unreadable]..
  21. CT/SPECT/PET imaging system for small animal studies
    Massoud Motamedi; Fiscal Year: 2007
    ..These efforts are of great and generalizable public health interest. [unreadable] [unreadable] [unreadable]..
  22. Analyses of InSitu and Explanted Surgical Implant Devices
    Jack Lemons; Fiscal Year: 2009
    ..abstract_text> ..
  23. Brain Biomarkers of Alcoholism and Abstinence
    Howard B Gutstein; Fiscal Year: 2010
    ....
  24. Microvascular Regulation During Intestinal Absorption
    HAROLD G BOHLEN; Fiscal Year: 2010
    ..A substantial fraction of the NO in blood may be from NO loading in the small arteries based on their very high wall NO concentration and represents a new form of vascular regulation. ..
  25. A Dual Electrospray/Photoionization Source
    JACK SYAGE; Fiscal Year: 2008
    ..There is already interest in this technology by commercial MS vendors. If we are successful with the Phase II work, we are confident of finding a commercialization partner for the ES/PI source. ..
  26. Microarraying and Screening Chemical Libraries
    Haiching Ma; Fiscal Year: 2009
    ..Overall, this instrumentation allows a large number of wild type or recombinant protein targets, each obtained in miniscule quantity, to be screened against 100,000 to 500,000 compounds of the NIH repository. ..
  27. Microwave-Assisted Electrospray Ionization for LC/MS
    JACK SYAGE; Fiscal Year: 2008
    ..Finally this project has the potential for explosive commercial growth, which will stimulate economic development. [unreadable] [unreadable] [unreadable]..
  28. High throughput ELISA chip for drug toxicity screening
    Haiching Ma; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  29. High-resolution optical imaging assessment of microbicide toxicity
    Massoud Motamedi; Fiscal Year: 2008
    ..We also plan to use the developed imaging protocol to assess the performance of novel microbicides in small animal models as new products are developed. ..
  30. 2008 Single Molecule Approaches to Biology Gordon Research Conference
    Taekjip Ha; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  31. PHYSICAL CHEMISTRY OF NUCLEIC ACIDS
    Carlos Bustamante; Fiscal Year: 2008
    ..Finally, in the experiments described here, mechanical force will be used as a new controllable variable to characterize the process of mechanochemical transduction in this motor enzyme. ..
  32. Proteomic Approaches to Alcoholism
    Howard Gutstein; Fiscal Year: 2006
    ..abstract_text> ..
  33. Enzyme Assay Chips for Apoptosis Discovery
    Haiching Ma; Fiscal Year: 2006
    ..New inhibitors of caspases may lead to advances in the treatment of neurodegenerative processes during acute injury or in chronic disease. ..
  34. Affinity-based Mass Spectrometry Protein Assays
    Dobrin Nedelkov; Fiscal Year: 2004
    ..abstract_text> ..
  35. PHYSICAL CHEMISTRY OF RECOMBINATIONAL INTERMEDIATES
    NADRIAN SEEMAN; Fiscal Year: 2004
    ..These studies are designed to test key hypotheses and make central observations on DNA structures and processes that are involved in genetic recombination. ..
  36. PRECISE ISOTOPE RATIO CHROMATOGRAPHY AND STABLE TRACERS
    JAMES BRENNA; Fiscal Year: 2004
    ..The successful accomplishment of these plans will yield new tools and experimental approaches for investigation of the impact of developmental factors and recent physiological history on development of chronic disease. ..
  37. Capillary Electrophoresis: Bioanalytical Applications
    FRANK GOMEZ; Fiscal Year: 2003
    ..abstract_text> ..
  38. High-Speed Neonatal Screening by Photoionization MS/MS
    JACK SYAGE; Fiscal Year: 2003
    ..The principal advantages are (i) minimization of ion suppression by LPPI vs ESI, (ii)increased rate of MS/MS analysis by QitTof vs triple quadrupole MS, and (iii) simplification of the sample preparation requirements. ..
  39. Prostate Cancer Chip
    Haiching Ma; Fiscal Year: 2002
    ..The Phase I activities will lead toward establishing chip chemistries and microfluidic technologies for a commercialized chip to aid in prostate cancer diagnostics. ..
  40. MICROVASCULAR CHARACTERISTICS OF DIABETES MELLITUS
    HAROLD BOHLEN; Fiscal Year: 2002
    ..Positive results will confirm that chronic insulin resistance predisposes the microvasculature to abnormalities of endothelial function. However, antioxidants and inhibition of beta II isoenzymes of PKC may protect endothelial function. ..
  41. Contrast enhanced spectroscopic detection of oral cancer
    Massoud Motamedi; Fiscal Year: 2002
    ....
  42. DIETARY ESSENTIAL FATTY ACID ACCUMULATION IN RETINA
    JAMES BRENNA; Fiscal Year: 2002
    ..Sub-normal development will be directly revealed via functional tests to assess the impact of low omega-3 fatty acid status on develop of vision acid status on development of vision and related health parameters. ..
  43. Dental Implant Science & Tech Transfer Satellite Meeting
    Jack Lemons; Fiscal Year: 2004
    ..Both organizations, IRG and WCOI-6 have approved the collaborations. Experience has been that 400 or more implant dentists attend the WCOI-6 and i00 or more researchers attend the IRG satellite sessions. ..
  44. Fostriecin and Related Protein Phosphatase Inhibitors
    Dale Boger; Fiscal Year: 2005
    ....
  45. Attention in Children with the 22q11 Deletion Syndrome
    Christina Sobin; Fiscal Year: 2005
    ....
  46. New APEX diagnostic for hereditary sensorineural hearing loss
    Phyllis Gardner; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  47. Microsystems to Manipulate Fibroblast Chemotaxis
    Maribel Vazquez; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  48. CHEMISTRY AND BIOCHEMISTRY OF GLYCOCONJUGATES
    YUAN LEE; Fiscal Year: 2005
    ..These studies are significant from the evolutionary standpoint, since the occurrence of alpha-Gal in glycoproteins is rare in birds, and the responsible transferase seems to be different from that of mammals. ..
  49. Microfluidics, Physics and Chemistry of Gordon Conference
    LAURIE LOCASCIO; Fiscal Year: 2005
    ....
  50. Bioanalytical Applications of Capillary Electrophoresis
    FRANK GOMEZ; Fiscal Year: 2005
    ..abstract_text> ..
  51. From drug Use to Addiction: Unearthing the Switches
    Howard Gutstein; Fiscal Year: 2005
    ..It is hoped that these findings will eventually lead to improved treatments for these devastating conditions. ..
  52. Development of SPR/MS protein array platform
    Dobrin Nedelkov; Fiscal Year: 2005
    ....
  53. Microsensor Arrays for Saliva Diagnostics
    DAVID WALT; Fiscal Year: 2005
    ..Much of the initial work will be directed at screening potentially useful sensing chemistries for their applicability to sensing components in saliva. ..
  54. ECM Molecules in Chondrocyte Mechanotransduction
    RONALD GRAFF; Fiscal Year: 2005
    ..Learning the role of specific ECM molecules in chondrocyte mechanotransduction will increase our understanding of the pathogenesis of osteoarthritis and may aid in designing novel therapies for slowing progression. ..
  55. Enzyme Assay Chips for Apoptosis Discovery
    Haiching Ma; Fiscal Year: 2002
    ..This technology can have research, medical, and industrial uses with respoect to the monitoring of intracellular enzyme states and identifying potential drug targets. ..