- Microfabricated arrays of femtoliter chambers allow single molecule enzymology
Nat Biotechnol 23:361-5. 2005
..The approach should be of interest for many ultrasensitive bioassays at the single-molecule level...
- Microfluidics in structural biology: smaller, faster em leader better
Department of Applied Physics, California Institute of Technology, MS 128 95, Pasadena, CA 91125, USA
Curr Opin Struct Biol 13:538-44. 2003
..Diverse applications ranging from time-resolved studies of protein folding to highly efficient protein crystal growth suggest that microfluidics may become an indispensable tool in biology...
- An artificial taste sensor based on conducting polymers
A Riul Júnior
Embrapa Instrumentação Agropecuária, CP 741, CEP 13560 970 São Carlos, SP, Brazil
Biosens Bioelectron 18:1365-9. 2003
..Brands of several commercial beverages could be easily distinguished without complex analysis, including the discrimination of waters, tastants and wines...
- Physics and applications of microfluidics in biology
David J Beebe
Department of Biomedical Engineering, University of Wisconsin, Madison 53706, USA
Annu Rev Biomed Eng 4:261-86. 2002
..The focus of this review is microscale phenomena and the use of the physics of the scale to create devices and systems that provide functionality useful to the life sciences...
- Micropattern formation in supported lipid membranes
Jay T Groves
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
Acc Chem Res 35:149-57. 2002
..Collectively, these preparative and analytical patterned membrane techniques offer a broad experimental platform for the study and utilization of lipid membranes...
- Cramming more sequencing reactions onto microreactor chips
John H Leamon
RainDance Technologies, Inc, 530 Whitfield Street, Guilford, Connecticut 06437, USA
Chem Rev 107:3367-76. 2007
- Digital concentration readout of single enzyme molecules using femtoliter arrays and Poisson statistics
David M Rissin
Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, Massachusetts 02155, USA
Nano Lett 6:520-3. 2006
..This approach should prove useful for single molecule enzymology and ultrasensitive bioassays. More generally, the ability to determine concentration by counting individual molecules offers a new approach to analysis of dilute solutions...
- Disposable real-time microPCR device: lab-on-a-chip at a low cost
Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Nanos 04 01, Singapore 138669
Mol Biosyst 2:292-8. 2006
..As the glass slip is disposable, cross-contamination from sample to sample is eliminated. The total cost of running the PCR is given by the value of the cover slip and its treatment...
- Micro total analysis systems. Latest advancements and trends
Petra S Dittrich
Institute for Analytical Sciences, Bunsen Kirchhoff Strasse 11, D 44139 Dortmund, Germany
Anal Chem 78:3887-908. 2006
- Microfluidic technologies in clinical diagnostics
Thomas H Schulte
Micronics, Inc, Redmond, WA, USA
Clin Chim Acta 321:1-10. 2002
..The laminar flow behavior of fluids in microfluidic devices must be accommodated in the design and development of clinical and bio-clinical miniaturized systems...
- Determination of carcinoembryonic antigen in human sera by integrated bead-bed immunoassay in a microchip for cancer diagnosis
Department of Applied Chemistry, School of Engineering, The University of Tokyo, Hongo, Bunkyo, Japan
Anal Chem 73:1213-8. 2001
..This microchip-based diagnosis system is the first microchip-based system that is practically useful for clinical diagnoses with short analysis time, high sensitivity, and easy procedures...
- Disposable immunosensor for the determination of domoic acid in shellfish
Dipartimento di Scienze e Tecnologie Chimiche, Universita Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
Biosens Bioelectron 20:190-6. 2004
..Very good recoveries were obtained, demonstrating the suitability of the proposed assay for accurate determination of the DA concentration in mussel samples...
- Continuous flow immunosensor for highly selective and real-time detection of sub-ppb levels of 2-hydroxybiphenyl by using surface plasmon resonance imaging
K Vengatajalabathy Gobi
Art, Science and Technology Center for Cooperative Research, Kyushu University, Kasuga shi, Fukuoka 816 8580, Japan
Biosens Bioelectron 20:350-7. 2004
..The SPR sensor for HBP is proved to be applicable in simultaneous detection of HBP and BaP in parallel with another SPR sensor for BaP...
- A portable automated system for trace gas sampling in the field and stable isotope analysis in the laboratory
Daniel E Theis
Paul Scherrer Institute, Laboratory of Atmospheric Chemistry, 5232 Villigen, Switzerland
Rapid Commun Mass Spectrom 18:2106-12. 2004
..Our system should therefore further facilitate research of trace gases in the context of the carbon cycle in the field, and opens many other possible applications with carbon- and possibly non-carbon-containing trace gases...
- Experimental equivalent cluster-size distributions in nanometric volumes of liquid water
Physikalisch Technische Bundesanstalt, Bundesallee 100, D 38116 Braunschweig, Germany
Radiat Prot Dosimetry 110:851-7. 2004
- Verification of performance with the automated direct optical TIRF immunosensor (River Analyser) in single and multi-analyte assays with real water samples
Institute of Physical and Theoretical Chemistry IPTC, Eberhard Karls University of Tuebingen, Auf der Morgenstelle 8, D 72076 Tuebingen, Germany
Biosens Bioelectron 20:743-52. 2004
..Robustness could be demonstrated by long-term stability tests of the biosensor surface. These studies show that the biosensor used offers the necessary reproducibility, precision, and robustness required for an analytical method...
- In-situ quantitative analysis of a prostate-specific antigen (PSA) using a nanomechanical PZT cantilever
Kyo Seon Hwang
Microsystem Research Center, Korea Institute of Science and Technology, Seongbuk gu, Seoul 136 761, Republic of Korea
Lab Chip 4:547-52. 2004
..The saturated resonance frequency shift of the cantilever was proportional to the PSA antigen concentration of analyte solution...
- High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems
IBM Research GmbH, Zurich Research Laboratory, Saumerstrasse 4, CH 8803 Ruschlikon, Switzerland
Lab Chip 4:563-9. 2004
..6 mm(2) on PDMS to detect TNF-alpha in 600 nL of a dendritic cell (DC) culture medium with a sensitivity of approximately 20 pg mL(-1)(1.14 pM)...
- Analysing a magnetic molecule detection system--computer simulation
Department of Physics, University of Bielefeld, Universitatsstrasse 25, 33501 Bielefeld, Germany
J Biotechnol 112:35-46. 2004
..We can present first calculations where the bead particles (signal source) and the magnetic layers (sensor device) are considered as a whole magnetic ensemble...
- Development of an optical immunosensor based on the fluorescence of Cyanine-5 for veterinarian diagnostics
Biosensors Laboratory, IBET ITQB Instituto de Biologia Experimental e Tecnológica Instituto de Tecnologia Química e Biológica, Apartado 12, P 2781 901 Oeiras, Portugal
Biotechnol Lett 26:993-7. 2004
..Photobleaching had no effect on the fluorescence. This model system was used to detect Brucella sp. infection and could quantify anti-Brucella sp. antibodies in ovine serum samples in the range from 0.005 to 0.11 mg ml(-1)...
- An ultrasensitive and stable potentiometric immunosensor
Scientific Generics Ltd, Harston Mill, Harston, Cambridge CB2 5NH, UK
Biosens Bioelectron 18:1385-90. 2003
..All these assays were performed using complex fluid samples and the presence of any non-specific binding has no significant effect on the final measurement. New assays can be transferred and optimised readily...
- Planar microcoil-based microfluidic NMR probes
Institute of Microelectronics and Microsystems, EPFL Swiss Federal Institute of Technology Lausanne, BM 3 108, Lausanne CH 1015, Switzerland
J Magn Reson 164:242-55. 2003
..Finally, based on the presented model and measured data, future performance of planar microcoil-based microfluidic NMR probes is extrapolated and discussed...
- A high-performance glucose biosensor based on monomolecular layer of glucose oxidase covalently immobilised on indium-tin oxide surface
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
Biosens Bioelectron 19:43-9. 2003
..These characteristics make it possible to achieve mass production of biosensor compatible with the current electronic integrated circuit manufacturing technologies...
- Nucleic acid purification using microfabricated silicon structures
Nathaniel C Cady
Department of Microbiology, Cornell University, 317 Stocking Hall, Ithaca, NY 14853, USA
Biosens Bioelectron 19:59-66. 2003
- Optical-transparent and flexible glucose sensor with ITO electrode
Department of Human and Information Science, School of Engineering, Tokai University, 1117 Kitakaname, Hiratsuka, Kanagawa 259 1292, Japan
Biosens Bioelectron 19:67-71. 2003
..The sensor was possible to be used for measuring glucose from 0.06 to 1.24 mmol/l (correlation coefficient: 0.999), including the reported concentration of tear glucose in normal (0.14 mmol/l), with good reproducibility...
- Development of a conductimetric biosensor using immobilised Rhodococcus ruber whole cells for the detection and quantification of acrylonitrile
P C J Roach
Biotechnology Centre, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, West Yorkshire, HD1 3DH, UK
Biosens Bioelectron 19:73-8. 2003
..The biosensor has been shown to be reproducible with respect to the data obtained over a number of days, and retains stability for a minimum period of at least 5 days before recalibration of the biosensor is required...
- A micromachined capillary electrophoresis chip with fully integrated electrodes for separation and electrochemical detection
Institute for Microtechnology, Technical University of Braunschweig, Alte Salzdahlumer Str 203, 38124 Braunschweig, Germany
Biosens Bioelectron 19:149-53. 2003
..All three oxidizable species could be detected in less than 70 s. Glucose was detected by performing an enzymatic reaction along the separation channel. The microsystem showed a very good reproducibility...
- Redox labelled avidin for enzyme sensor architectures
Laboratory for Micro and Nanotechnology, Paul Scherrer Institut, CH 5232 Villigen PSI, Switzerland
Biosens Bioelectron 19:239-47. 2003
..1 mM, respectively. The molecular architecture concept allows quick adaptation of the sensors to other analytes, and it provides a platform for arrays of sensors with different selectivity...
- Simultaneous determination of L- and D-methotrexate using a sequential injection analysis/amperometric biosensors system
Raluca Ioana Stefan
Department of Chemistry, University of Pretoria, Pretoria 0002, South Africa
Biosens Bioelectron 19:261-7. 2003
..The SIA/biosensors system can be used reliably on-line in synthesis process control, for the simultaneous assay of L- and D-Mtx with a frequency of 34 samples per hour...
- Detection of heavy metal ions using protein-functionalized microcantilever sensors
Life Sciences Division, Oak Ridge National Laboratory, Building 4500S, Oak Ridge, TN 37831 6123, USA
Biosens Bioelectron 19:411-6. 2003
..Thus, we demonstrate that microcantilever bending can be used to discriminate between metal ions that bind and do not bind to AgNt84-6 protein in real time...
- Nanoporous aluminum oxide as a novel support material for enzyme biosensors
Fraunhofer Institute for Mechanics of Materials, D 06120 Halle, Saale, Germany
J Nanosci Nanotechnol 3:375-9. 2003
..The achieved operational stability showed that alumina membranes can be used to construct enzyme-modified electrodes...
- Electrocatalytic reduction of chloramphenicol at multiwall carbon nanotube-modified electrodes
Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, China
J Nanosci Nanotechnol 3:401-5. 2003
..Interfering experiments show that the modified electrode has excellent selectivity for the detection of CAP. The modified electrode was used to determine CAP in eyedrops, and the recoveries were approximately 100%...
- Integrated optical-fiber capillary electrophoresis microchips with novel spin-on-glass surface modification
Che Hsin Lin
Department of Mechanical and Electro Mechanical Engineering, National Sun Yat Sen University, Taiwan, ROC
Biosens Bioelectron 20:83-90. 2004
- Nano- and pico-dispensing of fluids on planar substrates using SAW
Christoph J Strobl
Department für Physik and Center for NanoScience CeNS, Geschwister Scholl Platz 1, University of Munich, D 80539 Munich, Germany
IEEE Trans Ultrason Ferroelectr Freq Control 51:1432-6. 2004
..In addition to a detailed description of the technique, several examples of applications are presented...
- Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes
School of Materials Science, Japan Advanced Institute of Science and Technology, 1 1 Asahidai, Tatsunokuchi, Nomi gun, Ishikawa 923 1292, Japan
Biosens Bioelectron 20:1482-90. 2005
..Such a small device is surely a strong candidate for low-cost DNA amplification, and detected as little as 0.4 copies of target DNA...
- Rapid, femtomolar bioassays in complex matrices combining microfluidics and magnetoelectronics
S P Mulvaney
Naval Research Laboratory, Washington, DC 20375, USA
Biosens Bioelectron 23:191-200. 2007
- Microfluidic vias enable nested bioarrays and autoregulatory devices in Newtonian fluids
Emil P Kartalov
Department of Biochemistry and Molecular Biology, Keck School of Medicine and Norris Cancer Center, University of Southern California, NOR6346, 1441 Eastlake Avenue, Los Angeles, CA 90033, USA
Proc Natl Acad Sci U S A 103:12280-4. 2006
..We also describe microfluidic septa and their applications. Vias lay the foundation for a new generation of microfluidic devices...
- Nano-dispensing by electrospray for biotechnology
Maxim E Kuil
Biophysical Structure Chemistry, Leiden Institute for Chemistry, Leiden, The Netherlands
Biotechnol J 1:969-75. 2006
..Experimental results and the design of a novel spraying configuration, which can be incorporated in an optical microscope, are reported...
- Single-ion dosemeter based on floating gate memories
Department of Information Engineering, Padova University, Padova, Italy
Radiat Prot Dosimetry 122:457-9. 2006
..This paper discusses the opportunities to use this devices as single an ion dosemeter with sub-micrometer spatial resolution and capable of distinguish the impinging ion LET...
- On-chip acidification rate measurements from single cardiac cells confined in sub-nanoliter volumes
Igor A Ges
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA
Biomed Microdevices 10:347-54. 2008
..8 mM of Ca(2+). The NanoPhysiometer concept can be extended to study ischemia/reperfusion injury or disorders of other biological systems to identify strategies for treatment and possible pharmacological targets...
- PCB/polymer based micro-fluidic system for NMR spectroscopy for nanoliters sample volume
Institut des Nanotechnologies de Lyon INL, University Claude Bernard, Villeurbanne, 69622 France
Conf Proc IEEE Eng Med Biol Soc 2007:2968-71. 2007
..The fabrication process is described, and first results are reported. The tight sealing of the micro-fluidic system has been demonstrated and preliminary NMR experiment results are presented...
- Electromechanical transducers at the nanoscale: actuation and sensing of motion in nanoelectromechanical systems (NEMS)
K L Ekinci
Department of Aerospace and Mechanical Engineering, Boston University, Boston, MA 02215, USA
Small 1:786-97. 2005
..The currently available techniques to actuate and detect NEMS motion are introduced, and the accuracy, bandwidth, and robustness of these techniques are discussed...
- On-chip oligonucleotide ligation assay using one-dimensional microfluidic beads array for the detection of low-abundant DNA point mutations
State Key Laboratory of Chemo Biosensing and Chemometrics, Biomedical Engineering Center, College of Chemistry and Chemical Engineering, Key Laboratory for Bio Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, PR China
Biosens Bioelectron 23:945-51. 2008
..Additionally, this on-chip beads ligation approach also displayed other microfluidic-based advantages of simple handling (one sample injection per test), little reagent quantities, and low potential of contaminations...
- Temperature distribution effects on micro-CFPCR performance
Pin Chuan Chen
Center for Bio Modular Multi Scale Systems, Louisiana State University, Baton Rouge, LA 70803, USA
Biomed Microdevices 10:141-52. 2008
..Lab Chip 4:638, 2004) strictly due to the improved temperature distribution...
- Micropumps, microvalves, and micromixers within PCR microfluidic chips: Advances and trends
MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, No 55, Zhongshan Avenue West, Tianhe District, Guangzhou 510631, PR China
Biotechnol Adv 25:483-514. 2007
..Finally, general discussions on microvalves, micropumps, and micromixers for PCR chips are given. The microvalve/micropump/micromixers allow high levels of PCR chip integration and analytical throughput...
- A suspended membrane nanocalorimeter for ultralow volume bioanalysis
Erik A Johannessen
Department of Human Anatomy and Cell Biology, University of Liverpool, New Medical School, L69 3LT Liverpool, UK
IEEE Trans Nanobioscience 1:29-36. 2002
..The nanocalorimetric sensor has the potential for integration in an ultralow-volume high-density array format for the characterization of processes in which there is an exchange of heat...
- Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods
A V Nabok
Sheffield Hallam University, Materials and Engineering Research Institute, City Campus, Pond Street, Sheffield S1 1WB, UK
Biosens Bioelectron 22:885-90. 2007
..The QCM impedance measurements data showed anomalously large mass increase and film softening, most likely, due to the binding of large T-2 aggregates to antibodies...
- Automated Water Analyser Computer Supported System (AWACSS) Part II: Intelligent, remote-controlled, cost-effective, on-line, water-monitoring measurement system
Institute of Physical and Theoretical Chemistry IPTC, Eberhard Karls University of Tuebingen, Auf der Morgenstelle 8, Tuebingen 72076, Germany
Biosens Bioelectron 20:1509-19. 2005
- Electrochemical determination of reversible redox species at interdigitated array micro/nanoelectrodes using charge injection method
Department of Electrical and Computer Engineering and Computer Science, University of Cincinnati, Cincinnati, OH 45221 0030, USA
IEEE Trans Nanobioscience 4:164-9. 2005
..Using p-aminophenol as the redox species, approximately 4 x 10(-7) M and 6 x 10(-9) M detection limits on the species concentration ar respectively achieved with the microelectrodes and the nanoelectrodes...
- Catheter-tip sensor to monitor production of hydrogen peroxide in small biosamples
Chaitanya S Sathe
Applied Biology and Biomedical Engineering, Rose Hulman Institute of Technology, Terre Haute, Indiana 47803, USA
Biomed Sci Instrum 41:193-8. 2005
..7 microM rat IgG solution. We further discuss the usefulness, limitation and the future scope of this real time monitoring system for H2O2 research using small biosamples...
- Precise nanoliter fluid handling system with integrated high-speed flow sensor
Seyonic SA, Neuchatel, Switzerland
Assay Drug Dev Technol 3:203-12. 2005
..The collected dispense information can be used for quality control assessments and automatically be made part of an electronic record...
- Nanoliter dispensing for uHTS using pin tools
Patrick H Cleveland
V and P Scientific, Inc, San Diego, CA 92121, USA
Assay Drug Dev Technol 3:213-25. 2005
..The factors that affect liquid transfers by pin tools are discussed in detail as well as the advantages and limitations of pin tools...
- Amperometric biosensor based on tyrosinase-conjugated polysaccharide hybrid film: selective determination of nanomolar neurotransmitters metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC) in biological fluid
Energy Technology Research Institute, National Institute of Advanced Industrial Science and Technology AIST, Umezono 1 1 1, Tsukuba 305 8568, Japan
Biosens Bioelectron 21:809-16. 2005
..A physiological level of neurotransmitters and their derivatives including dopamine, l-dopa, adrenaline, noradrenaline and homovanillic acid as well as ascorbic acid, uric acid and acetaminophen do not affect the determination of DOPAC...
- Micro gas analysis system for measurement of atmospheric hydrogen sulfide and sulfur dioxide
Shin ichi Ohira
Department of Science, Kumamoto University, 2 39 1, Kurokami, Kumamoto, 860 8555, Japan
Lab Chip 5:1374-9. 2005
..The system we developed allowed automated continuous analyses in the field and achieved a much higher time resolution compared to those by ion chromatographic analysis...
- Cantilever sensor for nanomechanical detection of specific protein conformations
Interdisciplinary Nanoscience Center, Department of Physics and Astronomy, University of Aarhus, 8000 Aarhus C, Denmark
Nano Lett 5:2385-8. 2005
- Integrated potentiostat for neurotransmitter sensing. A high sensitivity, wide range VLSI design and chip
Johns Hopkins School of Medicine, Baltimore, Maryland, USA
IEEE Eng Med Biol Mag 24:23-9. 2005
- Electrohydrodynamic (EHD) dispensing of nanoliter DNA droplets for microarrays
Jeong Gun Lee
Samsung Advanced Institute of Technology, Mt 14 1, Nongseo ri, Giheung eup, Yongin si, Gyeonggi Do, Korea
Biosens Bioelectron 21:2240-7. 2006
..Consequently, it is found that EHD dispensing method can be used to make cost-effective DNA microarrays with no thermal and electrical influences on DNA properties...
- Miniature sensor suitable for electronic nose applications
Lal A Pinnaduwage
Oak Ridge National Laboratory, P O Box 2008, Oak Ridge, TN 37831 6122, USA
Rev Sci Instrum 78:055101. 2007
..The sensor can detect parts-per-trillion concentrations of DMMP within 10 s exposure times. The small size of the sensor makes it ideally suited for electronic nose applications...
- Single gallium nitride nanowire lasers
Justin C Johnson
Department of Chemistry, University of California, Berkeley, California 94720 1460, USA
Nat Mater 1:106-10. 2002
..This study is a considerable advance towards the realization of electron-injected, nanowire-based ultraviolet-blue coherent light sources...
- Probing distance and electrical potential within a protein pore with tethered DNA
Department of Medical Biochemistry and Genetics, The Texas A and M University System Health Science Center, 440 Reynolds Medical Building, College Station, TX 77843 1114, USA
Biophys J 83:3202-10. 2002
- Design and fabrication of a silica on silicon integrated optical biochip as a fluorescence microarray platform
Jesus M Ruano
Department of Electronics and Electrical Engineering, The University of Glasgow, Glasgow G12 8LT, UK
Biosens Bioelectron 18:175-84. 2003
..Finally, in this paper, we describe the implementation of a pumping system for delivering small amounts of fluid across the array together with an optical signal treatment...
- Optical biosensor for urea with improved response time
Department for General and Physical Chemistry, University of Pecs, 7624 Pecs, Ifjúság u 6, Hungary
Biosens Bioelectron 18:111-8. 2003
..Thirty sample/hour analysis-rate, good peak-to-peak reproducibility (RSD=0.02) and recovery (95-104%) was achieved with buffer diluted urea solutions. Applications for the analysis of real samples are planned to do in the future...
- High sensitivity detection of molecular recognition using magnetically labelled biomolecules and magnetoresistive sensors
D L Graham
INESC Microsystems and Nanotechnologies, R Alves Redol 9, 1000 Lisbon, Portugal
Biosens Bioelectron 18:483-8. 2003
..A wide range of applications is foreseen for this system in the development of biochips, high sensitivity biosensors and the detection of single molecules and single molecule interactions...
- Conventional detection of 2,4-dinitrophenol using quartz crystal microbalance
Jong Won Park
IEEE Trans Ultrason Ferroelectr Freq Control 50:193-5. 2003
..This QCM method allows detection of DNP concentration in the range of 0.01 to 100 ng/ml; linear correlation obtains DNP concentration from 1 to 100 ng/ml...
- Development and study of an amperometric biosensor for the in vitro measurement of low concentration of putrescine in blood
Hungarian Academy of Sciences, Research Group for Chemical Sensors, Ifjúság u 6, 7624 Pecs, Hungary
J Biochem Biophys Methods 53:165-75. 2002
..A detection limit of 50 nM was achieved in pH-adjusted whole blood samples, which is below pathological levels...
- Fabrication and evaluation of a carbon-based dual-electrode detector for poly(dimethylsiloxane) electrophoresis chips
A J Gawron
Department of Pharmaceutical Chemistry and Center for Bioanalytical Research, University of Kansas, Lawrence 66047, USA
Electrophoresis 22:242-8. 2001
..These devices illustrate the potential for a rugged and easily constructed microchip CE system with an integrated carbon-based detector of similar scale...
- Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P O Box 124, SE 221 00, Lund, Sweden
Biosens Bioelectron 18:547-53. 2003
..The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days...
- A fractal analysis of protein to DNA binding kinetics using biosensors
Chemical Engineering Department, University of Mississippi, Post Office Box 1848, University, MS 38677 1848, USA
Biosens Bioelectron 18:985-97. 2003
..Even though these reactions are occurring on the biosensor surface, the relationships presented should assist in understanding and in possibly manipulating the reactions occurring on cellular surfaces...
- An amperometric bi-enzyme sensor for determination of formate using cofactor regeneration
Karen K W Mak
Sino German Nano Analytical Lab SiGNAL and Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
Biosens Bioelectron 18:1095-100. 2003
..98 x 10(-7) M for formate (S/N=3), with the response time of 4 min. The working stability is limited to 7 days due to the inactivation of the enzymes. Only sodium salicylate was needed in milli-molar amounts...
- The shape parameter of liposomes and DNA-lipid complexes determined by viscometry utilizing small sample volumes
Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, China
Biophys J 85:1223-32. 2003
..7 as prolate ellipsoids or 4.9 as oblate ellipsoids. The deviation of shape from perfect sphere is thus expressed quantitatively in more than two significant figures. In these measurement, the necessary amount of DNA is <0.5 mg...
- Highly sensitive biosensing using a supercritical angle fluorescence (SAF) instrument
Physikalisch Chemisches Institut, Universitat Zurich, Winterthurerstr 190, CH 8057, Zurich, Switzerland
Biosens Bioelectron 18:1193-9. 2003
..The performance of the system is demonstrated by real time measurements of antibody-antigen reactions. Rate constants of the reaction were extracted. Antigen concentrations were detected down to 10(-13) mol/l...
- In situ TEM of radiation effects in complex ceramics
Department of Mechanical, Aerospace and Nuclear Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
Microsc Res Tech 72:165-81. 2009
..irradiations have been summarized with a focus on irradiation-induced microstructural evolution, changes in microchemistry, and the formation of nanostructures...
- Two new male contraceptives exert their effects by depleting germ cells prematurely from the testis
C Y Cheng
Population Council, The Rockefeller University, New York, New York 10021, USA
Biol Reprod 65:449-61. 2001
..In addition, results of serum microchemistry illustrate that liver and kidney function was not affected in animals treated with both compounds.
- Critical care medicine: opportunities and strategies for improvement
I L Cohen
Department of Surgery, Buffalo General Hospital, Buffalo, New York 14203, USA
Jt Comm J Qual Improv 22:85-103. 1996
..Like other areas of health care, critical care faces increasing pressure to improve the quality while reducing the cost of care. Strategies drawn from the literature and the authors' experiences are presented...
- Distinct migratory and non-migratory ecotypes of an endemic New Zealand eleotrid (Gobiomorphus cotidianus) - implications for incipient speciation in island freshwater fish species
Limnological Institute, University of Konstanz, Konstanz, Germany
BMC Evol Biol 8:49. 2008
- Alkali element uptake in otoliths: a link between the environment and otolith microchemistry
Lisa A Friedrich
Department of Geological Sciences, University of Manitoba, Winnipeg MB, Canada R3T 2N2
Environ Sci Technol 42:3514-8. 2008
..between the elements in the otoliths and surrounding rocks suggests there can be a strong link between the microchemistry of a fish's otoliths and its environment particularly where there are distinctive rock types...
- Larval quality is shaped by matrix effects: implications for connectivity in a marine metapopulation
Jeffrey S Shima
School of Biological Sciences, Coastal Ecology Lab, P O Box 600, Victoria University of Wellington, Wellington 6140, New Zealand
Ecology 90:1255-67. 2009
..We used laser ablation-inductively coupled plasma-mass spectrometry to quantify otolith microchemistry, followed by a set of cluster analyses (based upon 13 statistical descriptors of time series for each of 11 ..
- The legacy of dispersal: larval experience shapes persistence later in the life of a reef fish
Jeffrey S Shima
School of Biological Sciences, PO Box 600, Victoria University of Wellington, New Zealand Department of Zoology, University of Melbourne, VIC 3010 Australia
J Anim Ecol 79:1308-14. 2010
..2. We evaluated such 'legacy effects' for dispersing reef fish larvae using (i) otolith (ear stone) microchemistry to characterize two distinct dispersal pathways and (ii) otolith microstructure to estimate 'larval quality' (..
- Laser surface treatment of hydroxyapatite for enhanced tissue integration: surface characterization and osteoblastic interaction studies
Laboratorio de Biomateriais, INEB Instituto de Engenharia Biomedica, Rua do Campo Alegre 823, 4150 180, Porto, Portugal
J Biomed Mater Res A 81:920-9. 2007
Biocompatibility has long been associated with surface microtopography, microtexture, and microchemistry. The surface topography eventually affects the nature and the intensity of the interactions that occur at biomaterial-biological ..
- Interactions between MC3T3-E1 cells and textured Ti6Al4V surfaces
W O Soboyejo
Princeton Materials Institute, Princeton University, Princeton, New Jersey 08544, USA
J Biomed Mater Res 62:56-72. 2002
..The potential cytotoxic effects of microchemistry on cell-surface interactions also are considered in studies of cell spreading and orientation over 9-day ..
- Chemical composition of saccular endolymph and otolith in fish inner ear: lack of spatial uniformity
Laboratoire de Physiologie et Toxicologie Environnementales, EA 2138, France
Am J Physiol 277:R123-31. 1999
..Thus the lack of uniformity in the otolith composition must be taken into account when investigating otolith microchemistry.
- Biomaterials, biomechanics, tissue healing, and immediate-function dental implants
Jack E Lemons
Department of Prosthodontics and Biomaterials, University of Alabama at Birmingham, SDB Box 49, Birmingham, AL 35294 0007, USA
J Oral Implantol 30:318-24. 2004
..finn, and porous geometry endosteal dental-implant designs with and without alterations in device body-surface microchemistry and microtopography...
- A multi-locus assessment of connectivity and historical demography in the bluehead wrasse (Thalassoma bifasciatum)
R A Haney
Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, USA
Heredity (Edinb) 98:294-302. 2007
..previous studies have found both substantial levels of local recruitment, in studies interpreting otolith microchemistry and, conversely, a lack of genetic differentiation inferred from mitochondrial DNA (mtDNA) restriction-..
- High-precision continuous-flow isotope ratio mass spectrometry
J T Brenna
Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
Mass Spectrom Rev 16:227-58. 1997
..Capillary gas chromatography interfaced to IRMS via on-line microchemistry facilitates compound-specific isotope analysis (CSIA) for purified organic analytes of 1 nmol of C, N, or O...
- Photoemission and free electron laser spectromicroscopy: photoemission at high lateral resolution
Institut de Physique Appliquee, Ecole Polytechnique Federale, Lausanne, Switzerland
Scanning Microsc 9:949-63; discussion 963. 1995
..physics and biology, such as local chemical fluctuations in superconductors, semiconductor interfaces and the microchemistry of biological systems...
- Towards inexpensive DNA diagnostics
National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Trends Biotechnol 14:397-401. 1996
..This will require the efficient integration of technical advances in microchemistry, micromachining, separation technologies, detection systems, microelectronics and information technology, and ..
- Thermooptical detection in microchips: from macro- to micro-scale with enhanced analytical parameters
Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo, Japan
Electrophoresis 29:2741-53. 2008
..TLM showed low LODs in solvent extraction (down to 10(-8) M) and electrokinetic separation (10(-7) M), which were shown to be governed by discussed instrumental regularities, instead of by microchemistry.
- DEP actuated nanoliter droplet dispensing using feedback control
Kai Liang Wang
University of Rochester, Rochester, NY 14627, USA
Lab Chip 9:901-9. 2009
..Reconfiguring the module connections and reprogramming the control module permits testing of a variety of control strategies...
- Capillary circular dichroism
Daniel E Waldron
Department of Chemistry, University of Warwick, Coventry, United Kingdom
Chirality 22:E136-41. 2010
..The system performance was validated with Na[Co(EDDS)].H(2)O (EDDS = N,N-ethylenediaminedisuccinic acid), concanavalin A, lysozyme, and progesterone...
- Microfabricated device for DNA and RNA amplification by continuous-flow polymerase chain reaction and reverse transcription-polymerase chain reaction with cycle number selection
Pierre J Obeid
Department of Chemistry, University of Patras, Patras, Greece GR 26500
Anal Chem 75:288-95. 2003
..To our knowledge, this is the first report of a monolithic microdevice that integrates continuous-flow RT and PCR with cycle number selection...
- Velocity measurement of particles flowing in a microfluidic chip using Shah convolution Fourier transform detection
Y C Kwok
AstraZeneca/SmithKline Beecham Centre for Analytical Sciences, Department of Chemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, United Kingdom
Anal Chem 73:1748-53. 2001
..This technique could have wide applications, ranging from the determination of the velocity of particles in pressure-driven flow to the measurement of electrophoretic mobilities of single biological cells...
- Determination of trace metals in drinking water using solid-phase extraction disks and X-ray fluorescence spectrometry
Department of Chemistry, Wake Forest University, Winston Salem, North Carolina 27109, USA
Appl Spectrosc 57:338-42. 2003
..Thus, the overall cost for analysis of metals in drinking water can be minimized by implementing the method, and small water supply companies with limited budgets will be better able to comply with the Safe Drinking Water Act...
- Identification card and codification of the chemical and morphological characteristics of 14 dental implant surfaces
David M Dohan Ehrenfest
LoB5 unit, Chonnam National University School of Dentistry, Gwangju, South Korea
J Oral Implantol 37:525-42. 2011
..The DEC system could be an interesting basis for the development of a clear and simple ISO standard for dental implant surfaces and other implantable devices...
- A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative
J G Mohanty
MCP Hahnemann School of Medicine, Division of Pulmonary and Critical Care Medicine, Allegheny University of the Health Sciences, Philadelphia, PA 19102, USA
J Immunol Methods 202:133-41. 1997
..The protocol described in this study using A6550 to measure H2O2 release from activated granulocytes can be easily adapted to other cell types which generate H2O2...
- Preparation and application of graphene-poly (diallyldimethylammoniumchloride)-iron oxide nanoparticles buckypaper for hydrogen peroxide detection
School of Electrical Engineering, Korea University, 5 ga, Anam Dong, Seongbuk gu, Seoul 136 713, Korea
J Nanosci Nanotechnol 13:7349-57. 2013
..986, and a high sensitivity of 218 microA mM(-1) x cm(-2). Such low-cost G-PDDA-NP composite BPs prepared by facile methods pave way towards novel sensors with better performance...
- MEMS technology in analytical chemistry
Anil K Deisingh
Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4M1
Analyst 128:9-11. 2003
..Anil Deisingh talks us through the basics of MEMS technology and the growing role of these new "micromachines" in analytical chemistry applications...
- Design and applications of sensitive enzyme immunoassays specific for clostridial enoate reductases
, , Bundesrepublik Deutschland
Z Naturforsch C 44:345-52. 1989
..thermoaceticum to disintegrate into its subunits and fragments as compared to its mesophilic counterpart...
- Microrheology of the liquid-solid transition during gelation
Travis H Larsen
Department of Chemical Engineering and Center for Molecular and Engineering Thermodynamics, University of Delaware, Newark, Delaware 19716, USA
Phys Rev Lett 100:146001. 2008
..Both systems exhibit a critical relaxation exponent n approximately 0.6, where G' approximately G'' approximately omega n for the incipient gel, consistent with the Rouse model of dynamic scaling in the percolation universality class...
- Point-of-care testing
M R Fiallos
Division of Pediatrics, Department of Critical Care Medicine, University Community Hospital, Tampa, Florida, USA
Pediatr Clin North Am 48:589-99. 2001
..The constant evolution and developments in [figure: see text] microchemistry and computer technology will make this area a dynamic part of medicine with the constant emergence of improved ..
- Rapid screening of the aglycone specificity of glycosidases: applications to enzymatic synthesis of oligosaccharides
J E Blanchard
Protein Engineering Network of Centres of Excellence of Canada and the Department of Chemistry, University of British Columbia, V6T 1Z1, Vancouver, BC, Canada
Chem Biol 8:627-33. 2001
..CONCLUSIONS: The aglycone specificity of a glycosidase can be rapidly evaluated and requires only an appropriate fluorosugar inactivator, a substrate for assay of activity and a library of compounds for screening...
- A modular microfluidic architecture for integrated biochemical analysis
Kashan A Shaikh
Department of Electrical and Computer Engineering, Micro and Nanotechnology Laboratory, and Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
Proc Natl Acad Sci U S A 102:9745-50. 2005
..In the second case, we used a DNAzyme-based biosensor to identify the presence of Pb(2+) (lead) at a sensitivity of 500 nM in <1 nl of solution...