glucose clamp technique

Summary

Summary: Maintenance of a constant blood glucose level by perfusion or infusion with glucose or insulin. It is used for the study of metabolic rates (e.g., in glucose, lipid, amino acid metabolism) at constant glucose concentration.

Top Publications

  1. ncbi Homeostasis model assessment closely mirrors the glucose clamp technique in the assessment of insulin sensitivity: studies in subjects with various degrees of glucose tolerance and insulin sensitivity
    E Bonora
    Division of Endocrinology and Metabolic Diseases, University of Verona Medical School, Italy
    Diabetes Care 23:57-63. 2000
  2. ncbi Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage
    Ranganath Muniyappa
    Diabetes Unit, National Center for Complementary and Alternative Medicine, National Institutes of Health, 9 Memorial Drive, Bldg 9, Rm 1N 105 MSC 0920, Bethesda, MD 20892, USA
    Am J Physiol Endocrinol Metab 294:E15-26. 2008
  3. ncbi Lipid-induced insulin resistance in human muscle is associated with changes in diacylglycerol, protein kinase C, and IkappaB-alpha
    Samar I Itani
    Diabetes Unit, Section of Endocrinology, Department of Medicine, Boston University Medical Center, Boston, MA, USA
    Diabetes 51:2005-11. 2002
  4. ncbi Ceramide content is increased in skeletal muscle from obese insulin-resistant humans
    John M Adams
    Department of Physiology, The University of Texas Health Science Center at San Antonio, 78229 3900, USA
    Diabetes 53:25-31. 2004
  5. ncbi Overexpression of monocyte chemoattractant protein-1 in adipose tissues causes macrophage recruitment and insulin resistance
    Nozomu Kamei
    Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo, Tokyo 113 8655, Japan
    J Biol Chem 281:26602-14. 2006
  6. ncbi Impaired regulation of hepatic glucose production in mice lacking the forkhead transcription factor Foxo1 in liver
    Michihiro Matsumoto
    Naomi Berrie Diabetes Center, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
    Cell Metab 6:208-16. 2007
  7. ncbi Role of hepatic STAT3 in brain-insulin action on hepatic glucose production
    Hiroshi Inoue
    Department of Clinical Molecular Medicine, Division of Diabetes and Digestive and Kidney Diseases, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Cell Metab 3:267-75. 2006
  8. ncbi Considerations in the design of hyperinsulinemic-euglycemic clamps in the conscious mouse
    Julio E Ayala
    Vanderbilt NIDDK National Institutes of Diabetes and Digestive and Kidney Diseases Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Diabetes 55:390-7. 2006
  9. ncbi Prevention of obesity and insulin resistance in mice lacking plasminogen activator inhibitor 1
    Li Jun Ma
    Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    Diabetes 53:336-46. 2004
  10. pmc Hypothalamic leptin signaling regulates hepatic insulin sensitivity via a neurocircuit involving the vagus nerve
    Jonathan German
    Department of Medicine, University of Washington at South Lake Union, 815 Mercer Street, Box 358055, Seattle, Washington 98195
    Endocrinology 150:4502-11. 2009

Detail Information

Publications280 found, 100 shown here

  1. ncbi Homeostasis model assessment closely mirrors the glucose clamp technique in the assessment of insulin sensitivity: studies in subjects with various degrees of glucose tolerance and insulin sensitivity
    E Bonora
    Division of Endocrinology and Metabolic Diseases, University of Verona Medical School, Italy
    Diabetes Care 23:57-63. 2000
    ..To evaluate whether the homeostasis model assessment (HOMA) is a reliable surrogate measure of in vivo insulin sensitivity in humans...
  2. ncbi Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage
    Ranganath Muniyappa
    Diabetes Unit, National Center for Complementary and Alternative Medicine, National Institutes of Health, 9 Memorial Drive, Bldg 9, Rm 1N 105 MSC 0920, Bethesda, MD 20892, USA
    Am J Physiol Endocrinol Metab 294:E15-26. 2008
    ..In this Frontiers article, we highlight merits, limitations, and appropriate use of current in vivo measures of insulin sensitivity/resistance...
  3. ncbi Lipid-induced insulin resistance in human muscle is associated with changes in diacylglycerol, protein kinase C, and IkappaB-alpha
    Samar I Itani
    Diabetes Unit, Section of Endocrinology, Department of Medicine, Boston University Medical Center, Boston, MA, USA
    Diabetes 51:2005-11. 2002
    ..Whether acute FFA-induced insulin resistance in human skeletal muscle is caused by the activation of these specific PKC isoforms and the IKK-beta/IkappaB/NFkappaB pathway remains to be established...
  4. ncbi Ceramide content is increased in skeletal muscle from obese insulin-resistant humans
    John M Adams
    Department of Physiology, The University of Texas Health Science Center at San Antonio, 78229 3900, USA
    Diabetes 53:25-31. 2004
    ....
  5. ncbi Overexpression of monocyte chemoattractant protein-1 in adipose tissues causes macrophage recruitment and insulin resistance
    Nozomu Kamei
    Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo, Tokyo 113 8655, Japan
    J Biol Chem 281:26602-14. 2006
    ..We concluded that both paracrine and endocrine effects of MCP-1 may contribute to the development of insulin resistance in aP2-MCP-1 mice...
  6. ncbi Impaired regulation of hepatic glucose production in mice lacking the forkhead transcription factor Foxo1 in liver
    Michihiro Matsumoto
    Naomi Berrie Diabetes Center, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
    Cell Metab 6:208-16. 2007
    ..The data provide a unifying mechanism for regulation of hepatic glucose production by cAMP and insulin...
  7. ncbi Role of hepatic STAT3 in brain-insulin action on hepatic glucose production
    Hiroshi Inoue
    Department of Clinical Molecular Medicine, Division of Diabetes and Digestive and Kidney Diseases, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Cell Metab 3:267-75. 2006
    ..These results thus indicate that IL-6-STAT3 signaling in the liver contributes to insulin action in the brain, leading to the suppression of hepatic glucose production...
  8. ncbi Considerations in the design of hyperinsulinemic-euglycemic clamps in the conscious mouse
    Julio E Ayala
    Vanderbilt NIDDK National Institutes of Diabetes and Digestive and Kidney Diseases Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Diabetes 55:390-7. 2006
    ..kg(-1) . min(-1)), but endoR(a) was fully suppressed with doses higher than 0.8 mU . kg(-1) . min(-1). Thus, common variations in experimental factors yield different results and should be considered in designing and interpreting clamps...
  9. ncbi Prevention of obesity and insulin resistance in mice lacking plasminogen activator inhibitor 1
    Li Jun Ma
    Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA
    Diabetes 53:336-46. 2004
    ..Inhibition of PAI-1 might provide a novel anti-obesity and anti-insulin resistance treatment...
  10. pmc Hypothalamic leptin signaling regulates hepatic insulin sensitivity via a neurocircuit involving the vagus nerve
    Jonathan German
    Department of Medicine, University of Washington at South Lake Union, 815 Mercer Street, Box 358055, Seattle, Washington 98195
    Endocrinology 150:4502-11. 2009
    ..We conclude that hypothalamic leptin action increases peripheral insulin sensitivity primarily via effects on the liver and that the mechanism underlying this effect is dependent on the hepatic branch of the vagus nerve...
  11. pmc Dissociation between fatty liver and insulin resistance in humans carrying a variant of the patatin-like phospholipase 3 gene
    Konstantinos Kantartzis
    Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Medicine, Nephrology and Clinical Chemistry, University of Tubingen, Tubingen, Germany
    Diabetes 58:2616-23. 2009
    ..We investigated whether the SNP determines liver fat independently of visceral adiposity and whether it may even play a role in protecting from insulin resistance...
  12. pmc Restoration of muscle mitochondrial function and metabolic flexibility in type 2 diabetes by exercise training is paralleled by increased myocellular fat storage and improved insulin sensitivity
    Ruth C R Meex
    Department of Human Movement Sciences, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands
    Diabetes 59:572-9. 2010
    ..We examined whether exercise training could restore mitochondrial function and insulin sensitivity in patients with type 2 diabetes...
  13. pmc Prolonged fasting identifies skeletal muscle mitochondrial dysfunction as consequence rather than cause of human insulin resistance
    Joris Hoeks
    Department of Human Biology, School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands
    Diabetes 59:2117-25. 2010
    ..It is therefore anticipated that skeletal muscle mitochondrial function is maintained to accommodate increased fat oxidation unless factors secondary to insulin resistance exert negative effects on mitochondrial function...
  14. pmc Endoplasmic reticulum stress is reduced in tissues of obese subjects after weight loss
    Margaret F Gregor
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts, USA
    Diabetes 58:693-700. 2009
    ..Therefore, we tested whether these stress pathways were also present in obese human subjects and/or regulated by weight loss...
  15. ncbi Increased liver fat, impaired insulin clearance, and hepatic and adipose tissue insulin resistance in type 2 diabetes
    Anna Kotronen
    Department of Medicine, Division of Diabetes, University of Helsinki, Helsinki, Finland
    Gastroenterology 135:122-30. 2008
    ..We also examined whether insulin suppression of serum free fatty acid (FFA) correlates with liver fat...
  16. ncbi Body fat distribution and insulin resistance in healthy Asian Indians and Caucasians
    A Raji
    Endocrine Hypertension Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Endocrinol Metab 86:5366-71. 2001
    ..Alterations in body fat distribution--particularly increased visceral fat--may contribute to these abnormalities...
  17. ncbi Chronic treatment with sildenafil improves energy balance and insulin action in high fat-fed conscious mice
    Julio E Ayala
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 2200 Pierce Ave, 702 Light Hall, Nashville, TN 37232, USA
    Diabetes 56:1025-33. 2007
    ..These results show that phosphodiesterase-5 is a potential target for therapies aimed at preventing diet-induced energy imbalance and insulin resistance...
  18. ncbi Relationship between hepatic/visceral fat and hepatic insulin resistance in nondiabetic and type 2 diabetic subjects
    Amalia Gastaldelli
    Division of Diabetes, Department of Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
    Gastroenterology 133:496-506. 2007
    ..We examined the relationship between visceral/hepatic fat accumulation and hepatic IR/accelerated gluconeogenesis (GNG)...
  19. ncbi The assessment of insulin resistance in man
    T M Wallace
    The Oxford Centre for Diabetes, Endocrinology and Metabolism, The Radcliffe Infirmary, UK
    Diabet Med 19:527-34. 2002
    ..The ability to measure insulin resistance is important in order to understand the aetiopathology of Type 2 diabetes, to examine the epidemiology and to assess the effects of intervention...
  20. ncbi n-3 Fatty acids preserve insulin sensitivity in vivo in a peroxisome proliferator-activated receptor-alpha-dependent manner
    Susanne Neschen
    Yale University School of Medicine, Howard Hughes Medical Institute, Departments of Internal Medicine, The Anlyan Center, P O Box 9812, New Haven, CT 06536 8012, USA
    Diabetes 56:1034-41. 2007
    ..0001 vs. genotype control). These data support the hypothesis that n-3 fatty acids protect from high-fat diet-induced hepatic insulin resistance in a PPAR-alpha-and diacylglycerol-dependent manner...
  21. ncbi Meal and oral glucose tests for assessment of beta -cell function: modeling analysis in normal subjects
    Andrea Mari
    Consiglio Nazionale delle Ricerche Institute of Systems Science and Biomedical Engineering, 35127 Padua, Italy
    Am J Physiol Endocrinol Metab 283:E1159-66. 2002
    ..With cautious interpretation, a 2-h MT or a standard OGTT can be used as surrogates of 24-h tests in assessing spontaneous beta-cell function...
  22. pmc Intrahepatic fat, not visceral fat, is linked with metabolic complications of obesity
    Elisa Fabbrini
    Center for Human Nutrition and Atkins Center of Excellence in Obesity Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 106:15430-5. 2009
    ..Furthermore, alterations in tissue fatty acid transport could be involved in the pathogenesis of ectopic triglyceride accumulation by redirecting plasma fatty acid uptake from adipose tissue toward other tissues...
  23. pmc Overexpression of carnitine palmitoyltransferase-1 in skeletal muscle is sufficient to enhance fatty acid oxidation and improve high-fat diet-induced insulin resistance
    Clinton R Bruce
    Cellular and Molecular Metabolism Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia
    Diabetes 58:550-8. 2009
    ....
  24. pmc Liver, muscle, and adipose tissue insulin action is directly related to intrahepatic triglyceride content in obese subjects
    Kevin M Korenblat
    Center for Human Nutrition, Washington University School of Medicine, St Louis, Missouri, USA
    Gastroenterology 134:1369-75. 2008
    ....
  25. pmc Intracerebroventricular administration of neuropeptide Y induces hepatic insulin resistance via sympathetic innervation
    Anita M van den Hoek
    TNO Quality of Life, Gaubius Laboratory, Leiden, The Netherlands
    Diabetes 57:2304-10. 2008
    ..Therefore, the aim of this study was to establish whether intracerebroventricular NPY administration modulates the suppressive action of insulin on EGP via hepatic sympathetic or parasympathetic innervation...
  26. ncbi Metformin prevents the development of acute lipid-induced insulin resistance in the rat through altered hepatic signaling mechanisms
    Mark E Cleasby
    Diabetes and Obesity Program, Garvan Institute of Medical Research, Sydney, Australia
    Diabetes 53:3258-66. 2004
    ..In summary, metformin opposes the development of acute lipid-induced insulin resistance in the liver through alterations in multiple signaling pathways...
  27. ncbi Insulin resistance and secretion in vivo: effects of different antipsychotics in an animal model
    Araba F Chintoh
    Institute of Medical Science, University of Toronto, 1 King s College Circle, Toronto, Ontario, Canada
    Schizophr Res 108:127-33. 2009
    ..Our data also support the preclinical use of these clamp procedures in screening putative antipsychotics...
  28. ncbi Hepatic insulin resistance precedes the development of diabetes in a model of intrauterine growth retardation
    Patricia Vuguin
    Children s Hospital at Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA
    Diabetes 53:2617-22. 2004
    ....
  29. ncbi Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle
    Lucy H Clerk
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Virginia Health System, P O Box 801410, 450 Ray C Hunt Drive, Charlottesville, VA 22908, USA
    Diabetes 55:1436-42. 2006
    ..482, P = 0.027) in response to insulin. In conclusion, obesity eliminated the insulin-stimulated muscle microvascular recruitment and increased brachial artery blood flow seen in lean individuals...
  30. ncbi Metabolic adaptations to dexamethasone-induced insulin resistance in healthy volunteers
    Nathalie Nicod
    Institute of Physiology, University of Lausanne, Lausanne, Switzerland
    Obes Res 11:625-31. 2003
    ..The objective of this study was to evaluate a procedure aimed at assessing the compensatory mechanisms to insulin resistance...
  31. pmc Muscle-derived angiopoietin-like protein 4 is induced by fatty acids via peroxisome proliferator-activated receptor (PPAR)-delta and is of metabolic relevance in humans
    Harald Staiger
    Department of Internal Medicine, Division of Endocrinology, Diabetology, Angiology, Nephrology, and Clinical Chemistry, Eberhard Karls University Tubingen, Tubingen, Germany
    Diabetes 58:579-89. 2009
    ..Long-chain fatty acids (LCFAs) contribute to metabolic homeostasis in part via gene regulation. This study's objective was to identify novel LCFA target genes in human skeletal muscle cells (myotubes)...
  32. pmc Metabolic flexibility in response to glucose is not impaired in people with type 2 diabetes after controlling for glucose disposal rate
    Jose E Galgani
    Pennington Biomedical Research Center, 6400 Perkins Rd, Baton Rouge, LA 70808, USA
    Diabetes 57:841-5. 2008
    ....
  33. ncbi Insulin sensitivity differs among ethnic groups with a compensatory response in beta-cell function
    K C Chiu
    Department of Medicine, University of California, Los Angeles, School of Medicine, USA
    Diabetes Care 23:1353-8. 2000
    ..A drastic difference is evident in the prevalence of type 2 diabetes among ethnic groups. We examined the role of beta-cell function and insulin sensitivity in this disparity among 4 ethnic groups...
  34. ncbi 13C/31P NMR studies on the mechanism of insulin resistance in obesity
    K F Petersen
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520 8020, USA
    Diabetes 47:381-6. 1998
    ..We conclude that insulin resistance in obesity can be mostly attributed to impairment of insulin-stimulated muscle glycogen synthesis due to a defect in glucose transport and/or phosphorylation activity...
  35. ncbi Minimal model estimation of glucose absorption and insulin sensitivity from oral test: validation with a tracer method
    Chiara Dalla Man
    Department of Information Engineering, University of Padova, Via Gradenigo 6 b, I 35131, Italy
    Am J Physiol Endocrinol Metab 287:E637-43. 2004
    ..86 (P < 0.0001). We conclude that OMM candidates as a reliable tool to measure both the rate of glucose absorption and insulin sensitivity from oral glucose tests without employing tracers...
  36. ncbi Effects of adding exercise to a 16-week very low-calorie diet in obese, insulin-dependent type 2 diabetes mellitus patients
    Marieke Snel
    Department of Endocrinology and Metabolism, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    J Clin Endocrinol Metab 97:2512-20. 2012
    ..Reduction of 50% excess body weight, using a very low-calorie diet (VLCD; 450 kcal/d) improves insulin sensitivity in obese type 2 diabetes mellitus patients...
  37. pmc Combining short-term metformin treatment and one bout of exercise does not increase insulin action in insulin-resistant individuals
    Carrie G Sharoff
    Department of Kinesiology, University of Massachusetts Amherst, USA
    Am J Physiol Endocrinol Metab 298:E815-23. 2010
    ..These findings suggest that the combination of short-term metformin treatment and an acute bout of exercise does not enhance insulin sensitivity, and the addition of metformin may attenuate the well-documented effects of exercise alone...
  38. ncbi Mechanisms of recovery from type 2 diabetes after malabsorptive bariatric surgery
    Caterina Guidone
    Dipartimento di Medicina Interna, Catholic University, Largo A Gemelli, 8, 00168 Rome, Italy
    Diabetes 55:2025-31. 2006
    ..This operation may affect the enteroinsular axis function by diverting nutrients away from the proximal gastrointestinal tract and by delivering incompletely digested nutrients to the ileum...
  39. ncbi Mice with gene disruption of both endothelial and neuronal nitric oxide synthase exhibit insulin resistance
    R R Shankar
    Department of Pediatrics, Indiana University School of Medicine, Indianapolis, USA
    Diabetes 49:684-7. 2000
    ..These data indicate that NO plays a role in modulating insulin sensitivity and carbohydrate metabolism and that the eNOS isoform may play a dominant role relative to nNOS...
  40. pmc Characterization of the metabolic and physiologic response to chromium supplementation in subjects with type 2 diabetes mellitus
    William T Cefalu
    Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA
    Metabolism 59:755-62. 2010
    ..Thus, modulation of lipid metabolism by Cr in peripheral tissues may represent a novel mechanism of action...
  41. pmc Estrogen sulfotransferase regulates body fat and glucose homeostasis in female mice
    Victor K Khor
    Department of Medicine and Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Am J Physiol Endocrinol Metab 299:E657-64. 2010
    ..In contrast, hepatic insulin sensitivity was improved but muscle insulin sensitivity did not change in EST transgenic mice. These results reveal novel effects of EST on adipose tissue and glucose homeostasis in female mice...
  42. ncbi Relationship between testosterone levels, insulin sensitivity, and mitochondrial function in men
    Nelly Pitteloud
    Reproductive Endocrine Unit, Massachusetts General Hospital, Boston, MA 02114, USA
    Diabetes Care 28:1636-42. 2005
    ..The goal of this study was to examine the relationship between serum testosterone levels and insulin sensitivity and mitochondrial function in men...
  43. ncbi Insulin resistance in patients with chronic kidney disease
    Shuzo Kobayashi
    Department of Nephrology and Kidney and Dialysis Center, Shonan Kamakura General Hospital, Kamakura, Japan
    Am J Kidney Dis 45:275-80. 2005
    ..However, few data are available concerning what factors of metabolic abnormalities, such as apolipoprotein (Apo) profile or acidosis, might be associated with insulin resistance in patients with CKD...
  44. pmc Physical inactivity and obesity underlie the insulin resistance of aging
    Francesca Amati
    Department of Health and Physical Activity, School of Education, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes Care 32:1547-9. 2009
    ..CONCLUSIONS Insulin resistance may not be characteristic of aging but rather associated with obesity and physical inactivity...
  45. ncbi Physical training may enhance beta-cell function in type 2 diabetes
    Flemming Dela
    Copenhagen Muscle Research Centre, The Panum Institute, University of Copenhagen, 2200 Copenhagen N, Denmark
    Am J Physiol Endocrinol Metab 287:E1024-31. 2004
    ..The improved beta-cell function does not require changes in insulin sensitivity and Hb A(1c) concentration...
  46. pmc Effects of insulin on the metabolic control of hepatic gluconeogenesis in vivo
    Dale S Edgerton
    Vanderbilt University Medical Center, Nashville, Tennessee, USA
    Diabetes 58:2766-75. 2009
    ....
  47. ncbi Relation of direct and surrogate measures of insulin resistance to cardiovascular risk factors in nondiabetic finnish offspring of type 2 diabetic individuals
    Carlos Lorenzo
    Department of Medicine, University of Texas Health Science Center, Room 5 594U, San Antonio, Texas 78229, USA
    J Clin Endocrinol Metab 95:5082-90. 2010
    ..Methods to directly measure insulin resistance are invasive, complex, and costly. Surrogate indexes derived from the oral glucose tolerance test (OGTT) have been developed, but few studies have systematically analyzed these indexes...
  48. ncbi Importance of quantifying insulin secretion in relation to insulin sensitivity to accurately assess beta cell function in clinical studies
    Bo Ahren
    Department of Medicine, Lund University, Lund, Sweden
    Eur J Endocrinol 150:97-104. 2004
    ..Acknowledging this inverse relationship between insulin secretion and insulin sensitivity therefore requires estimation of both variables for correct assessment in any individual...
  49. pmc Gene variants in the novel type 2 diabetes loci CDC123/CAMK1D, THADA, ADAMTS9, BCL11A, and MTNR1B affect different aspects of pancreatic beta-cell function
    Annemarie M Simonis-Bik
    Diabetes Center, VU University Medical Center, Amsterdam, The Netherlands
    Diabetes 59:293-301. 2010
    ..In this study we used hyperglycemic clamps with three different stimuli to test associations between these novel loci and various measures of beta-cell function...
  50. pmc Impaired mitochondrial activity in the insulin-resistant offspring of patients with type 2 diabetes
    Kitt Falk Petersen
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA
    N Engl J Med 350:664-71. 2004
    ..Insulin resistance appears to be the best predictor of the development of diabetes in the children of patients with type 2 diabetes, but the mechanism responsible is unknown...
  51. pmc Insulin resistance, defective insulin-mediated fatty acid suppression, and coronary artery calcification in subjects with and without type 1 diabetes: The CACTI study
    Irene E Schauer
    Department of Medicine, University of Colorado Denver, Aurora, USA
    Diabetes 60:306-14. 2011
    ..To assess insulin action on peripheral glucose utilization and nonesterified fatty acid (NEFA) suppression as a predictor of coronary artery calcification (CAC) in patients with type 1 diabetes and nondiabetic controls...
  52. ncbi Metabolic effects of visceral fat accumulation in type 2 diabetes
    Amalia Gastaldelli
    Metabolism Unit, Department of Internal Medicine, University of Pisa, Via Savi, I 56100 Pisa, Italy
    J Clin Endocrinol Metab 87:5098-103. 2002
    ..05). We conclude that in patients with established type 2 diabetes, VF accumulation has a significant negative impact on glycemic control through a decrease in peripheral insulin sensitivity and an enhancement of gluconeogenesis...
  53. ncbi Efficacy and safety of leptin-replacement therapy and possible mechanisms of leptin actions in patients with generalized lipodystrophy
    Ken Ebihara
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kyoto 606 8507, Japan
    J Clin Endocrinol Metab 92:532-41. 2007
    ....
  54. ncbi Prenatal and postnatal pathways to obesity: different underlying mechanisms, different metabolic outcomes
    Nichola M Thompson
    Liggins Institute, The University of Auckland, Private Bag 92019, Auckland, New Zealand
    Endocrinology 148:2345-54. 2007
    ..The origin of insulin hypersecretion in IUGR offspring may be independent of the mechanistic events that trigger the insulin resistance commonly observed in DIO...
  55. ncbi Effects of hyperglycemia and hyperinsulinemia on circulating tissue factor procoagulant activity and platelet CD40 ligand
    Vijender R Vaidyula
    Temple University Hospital, 3401 North Broad Street, Philadelphia, PA 19140, USA
    Diabetes 55:202-8. 2006
    ....
  56. ncbi Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta)
    H Cho
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 292:1728-31. 2001
    ..These data establish Akt2 as an essential gene in the maintenance of normal glucose homeostasis...
  57. ncbi Validation of insulin sensitivity indices from oral glucose tolerance test parameters in obese children and adolescents
    Catherine W Yeckel
    Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Clin Endocrinol Metab 89:1096-101. 2004
    ..74 and -0.71; P < 0.0001), a tissue marker for insulin resistance. Insulin sensitivity can be estimated using plasma glucose and insulin responses derived from the OGTT in obese youth with normal and impaired glucose tolerance...
  58. pmc Reduced mitochondrial density and increased IRS-1 serine phosphorylation in muscle of insulin-resistant offspring of type 2 diabetic parents
    Katsutaro Morino
    Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06536 8012, USA
    J Clin Invest 115:3587-93. 2005
    ....
  59. ncbi Insulin resistance after a 72-h fast is associated with impaired AS160 phosphorylation and accumulation of lipid and glycogen in human skeletal muscle
    M H Vendelbo
    Department of Clinical Pharmacology, Aarhus University Hospital NBG, Aarhus C, Denmark
    Am J Physiol Endocrinol Metab 302:E190-200. 2012
    ..This impairment of AS160 phosphorylation, in combination with glycogen accumulation and increased intramuscular lipid content, may provide the underlying mechanisms for resistance to insulin in skeletal muscle after a prolonged fast...
  60. ncbi Insulin secretion and action in subjects with impaired fasting glucose and impaired glucose tolerance: results from the Veterans Administration Genetic Epidemiology Study
    Muhammad A Abdul-Ghani
    Diabetes Division, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    Diabetes 55:1430-5. 2006
    ..Differences in insulin sensitivity and insulin secretion may predict different rates of progression to type 2 diabetes and varying susceptibility to cardiovascular disease...
  61. ncbi Alpha cell function in health and disease: influence of glucagon-like peptide-1
    B E Dunning
    PharmaWrite, LLC, Princeton, NJ, USA
    Diabetologia 48:1700-13. 2005
    ..Therapeutic approaches based around GLP-1 have the potential to improve both alpha cell and beta cell function, and could be of benefit in patients with a broad range of metabolic disorders...
  62. ncbi Effects of olanzapine and haloperidol on the metabolic status of healthy men
    Solrun Vidarsdottir
    Department of Endocrinology and Metabolism, Leiden University Medical Center, Leiden, The Netherlands
    J Clin Endocrinol Metab 95:118-25. 2010
    ..The aim of this study was to determine the effect of short-term olanzapine (atypical antipsychotic drug) and haloperidol (conventional antipsychotic drug) treatment on glucose and lipid metabolism...
  63. pmc Declining beta-cell function relative to insulin sensitivity with increasing fasting glucose levels in the nondiabetic range in children
    Hala Tfayli
    Division of Weight Management and Wellness, Children s Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Diabetes Care 33:2024-30. 2010
    ..This investigation tested the hypothesis that beta-cell function relative to insulin sensitivity decreases with increasing FPG in youth...
  64. ncbi Plasma adiponectin concentration is associated with skeletal muscle insulin receptor tyrosine phosphorylation, and low plasma concentration precedes a decrease in whole-body insulin sensitivity in humans
    Norbert Stefan
    Clinical Diabetes and Nutrition Section, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Phoenix, Arizona, USA
    Diabetes 51:1884-8. 2002
    ..Prospectively, low plasma adiponectin concentration at baseline precedes a decrease in insulin sensitivity. Our data indicate that adiponectin plays an important role in regulation of insulin sensitivity in humans...
  65. ncbi Relationship between insulin sensitivity and sphingomyelin signaling pathway in human skeletal muscle
    Marek Straczkowski
    Department of Endocrinology, Diabetology and Internal Medicine, Medical University Bialystok, M C Sklodowskiej 24a, 15 276 Bialystok, Poland
    Diabetes 53:1215-21. 2004
    ..68, P = 0.03) and linoleic (r = -0.66, P = 0.038) acid. Our data indicate that the sphingomyelin signaling pathway in muscle might be an important factor determining the development of insulin resistance in humans...
  66. ncbi Hypoglycemia-associated autonomic failure in advanced type 2 diabetes
    Scott A Segel
    Division of Endocrinology, Diabetes and Metabolism, and the General Clinical Research Center and the Diabetes Research and Training Center, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Diabetes 51:724-33. 2002
    ....
  67. ncbi Intramyocellular triglyceride content is a determinant of in vivo insulin resistance in humans: a 1H-13C nuclear magnetic resonance spectroscopy assessment in offspring of type 2 diabetic parents
    G Perseghin
    Division of Internal Medicine, Istituto Scientifico H San Raffaele, Milan, Italy
    Diabetes 48:1600-6. 1999
    ....
  68. ncbi A sustained increase in plasma free fatty acids impairs insulin secretion in nondiabetic subjects genetically predisposed to develop type 2 diabetes
    Sangeeta Kashyap
    Diabetes Division, Department of Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284 3900, USA
    Diabetes 52:2461-74. 2003
    ....
  69. ncbi Brain-derived neurotrophic factor (BDNF) and type 2 diabetes
    K S Krabbe
    The Centre of Inflammation and Metabolism, Department of Infectious Diseases, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    Diabetologia 50:431-8. 2007
    ..These disorders are associated with type 2 diabetes, and animal models suggest that BDNF plays a role in insulin resistance. We therefore explored whether BDNF plays a role in human glucose metabolism...
  70. pmc Bioactives in blueberries improve insulin sensitivity in obese, insulin-resistant men and women
    April J Stull
    Center for the Study of Botanicals and Metabolic Syndrome, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA
    J Nutr 140:1764-8. 2010
    ..In conclusion, daily dietary supplementation with bioactives from whole blueberries improved insulin sensitivity in obese, nondiabetic, and insulin-resistant participants...
  71. ncbi Exercise training increases insulin-stimulated glucose disposal and GLUT4 (SLC2A4) protein content in patients with type 2 diabetes
    D J O'Gorman
    Metabolic Research Unit, St James s Hospital, Trinity College, Dublin, Ireland
    Diabetologia 49:2983-92. 2006
    ..The aim of this study was to assess the impact of acute and short-term exercise training on whole-body insulin-mediated glucose disposal and signal transduction along the canonical insulin signalling cascade...
  72. pmc Proteomics analysis of human skeletal muscle reveals novel abnormalities in obesity and type 2 diabetes
    Hyonson Hwang
    Center for Metabolic Biology, Arizona State University, Tempe, Arizona, USA
    Diabetes 59:33-42. 2010
    ..However, changes in mRNA levels may not predict changes in protein abundance. An approach to identify global protein abundance changes involving the use of proteomics was used here...
  73. ncbi Comparison of insulin sensitivity, clearance, and secretion estimates using euglycemic and hyperglycemic clamps in children
    Gabriel I Uwaifo
    Unit on Growth and Obesity, Developmental Endocrinology Branch, National Institutes of Child Health and Human Development, National Institutes of Health, 10 Center Drive, Building 10 Room 10N262, Bethesda, MD 20892 1862, USA
    J Clin Endocrinol Metab 87:2899-905. 2002
    ..It cannot be assumed that the hyperglycemic clamp obviates the need for euglycemic clamp studies to accurately estimate insulin sensitivity in children...
  74. pmc Oral disposition index in obese youth from normal to prediabetes to diabetes: relationship to clamp disposition index
    Lindsey George Sjaarda
    Division of Weight Management and Wellness, Children s Hospital of Pittsburgh, University of Pittsburgh Medical Center, Pittsburgh, PA 15224, USA
    J Pediatr 161:51-7. 2012
    ....
  75. ncbi Insulin signaling in human skeletal muscle: time course and effect of exercise
    J F Wojtaszewski
    Copenhagen Muscle Research Centre, August Krogh Institute, University of Copenhagen, Denmark
    Diabetes 46:1775-81. 1997
    ..Thus, the activity of IRS-1-associated PI 3-kinase and glucose uptake may not always be tightly coupled during insulin stimulation in human muscle...
  76. ncbi Gender differences in renal responses to hyperglycemia and angiotensin-converting enzyme inhibition in diabetes
    David Z I Cherney
    Division of Nephrology, Toronto General Hospital, Toronto, Ontario, Canada
    Kidney Int 68:1722-8. 2005
    ....
  77. ncbi The DGAT2 gene is a candidate for the dissociation between fatty liver and insulin resistance in humans
    Konstantinos Kantartzis
    Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Medicine, Nephrology and Clinical Chemistry, University of Tubingen, 72076 Tubingen, Germany
    Clin Sci (Lond) 116:531-7. 2009
    ..This finding may be important in the prevention and treatment of insulin resistance and Type 2 diabetes in subjects with fatty liver...
  78. ncbi Effects of olanzapine and ziprasidone on glucose tolerance in healthy volunteers
    Julia Sacher
    Department of Psychiatry and Psychotherapy, Medical University of Vienna, Vienna, Austria
    Neuropsychopharmacology 33:1633-41. 2008
    ..Our finding is suggestive that not all atypical antipsychotics cause acute direct effects on glucose disposal and that accurate determination of side effect profile should be performed when choosing an atypical antipsychotic...
  79. ncbi Comparison of simple indices of insulin sensitivity using the euglycemic hyperinsulinemic clamp technique
    Marek Straczkowski
    Department of Endocrinology, Diabetology and Internal Medicine, Medical University, Białystok, Poland
    Med Sci Monit 10:CR480-4. 2004
    ..Therefore, indirect indices of insulin sensitivity are proposed. The aim of the present study was to compare these simple indices with data from clamp studies...
  80. ncbi Liver-specific suppressor of cytokine signaling-3 deletion in mice enhances hepatic insulin sensitivity and lipogenesis resulting in fatty liver and obesity
    Nirupa Sachithanandan
    St Vincent s Institute of Medical Research and Department of Medicine, University of Melbourne, Fitzroy, Victoria, Australia
    Hepatology 52:1632-42. 2010
    ..SOCS3 LKO mice fed an HFD also had elevated hypothalamic SOCS3 and fatty acid synthase expression and developed greater obesity due to increased food intake and reduced energy expenditure...
  81. ncbi Physiological and molecular determinants of insulin action in the baboon
    Alberto O Chavez
    Diabetes Division, Department of Medicine, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, San Antonio, TX 78229, USA
    Diabetes 57:899-908. 2008
    ..To quantitate insulin sensitivity in lean and obese nondiabetic baboons and examine the underlying cellular/molecular mechanisms responsible for impaired insulin action to characterize a baboon model of insulin resistance...
  82. pmc Surgical removal of omental fat does not improve insulin sensitivity and cardiovascular risk factors in obese adults
    Elisa Fabbrini
    Center for Human Nutrition and Atkins Center of Excellence in Obesity Medicine, Washington University School of Medicine, St Louis, Missouri, USA
    Gastroenterology 139:448-55. 2010
    ..Therefore, a reduction in VAT is considered an important target of obesity therapy. We evaluated whether reducing VAT mass by surgical removal of the omentum improves insulin sensitivity and metabolic function in obese patients...
  83. ncbi Brain glucose concentrations in poorly controlled diabetes mellitus as measured by high-field magnetic resonance spectroscopy
    Elizabeth R Seaquist
    Department of Medicine, University of Minnesota Medical School, MN 55455, USA
    Metabolism 54:1008-13. 2005
    ..1 micromol/g wet wt; P = .1). Our sample size gave 80% power to detect a difference as small as 1.1 micromol/g wet wt. We conclude that chronic hyperglycemia in diabetes does not alter brain glucose concentrations in human subjects...
  84. ncbi Effects of feed restriction and cold exposure on glucose metabolism in response to feeding and insulin in sheep
    H Sano
    Department of Bioscience and Technology, Faculty of Agriculture, Iwate University, Morioka, Japan
    J Anim Sci 77:2564-73. 1999
    ..Moreover, the action of insulin on glucose metabolism is enhanced during cold exposure, and the effect of feed restriction is somewhat enhanced...
  85. pmc Glucose prevents the fall in ventromedial hypothalamic GABA that is required for full activation of glucose counterregulatory responses during hypoglycemia
    Wanling Zhu
    Section of Endocrinology, Department of Internal Medicine, Yale University School of Medicine, 300 Cedar St, New Haven, CT, USA
    Am J Physiol Endocrinol Metab 298:E971-7. 2010
    ....
  86. ncbi Carotid artery intima-media thickness is associated with insulin-mediated glucose disposal in nondiabetic normotensive offspring of type 2 diabetic patients
    Marina Cardellini
    Dipartimento di Medicina Sperimentale e Clinica, Policlinico Universitario Mater Domini, Via Europa, Campus Germaneto, 88100 Catanzaro, Italy
    Am J Physiol Endocrinol Metab 292:E347-52. 2007
    ..7% of its variation. These findings support the concept that insulin sensitivity, rather than plasma insulin levels, is associated with early atherosclerosis in nondiabetic normotensive offspring of type 2 diabetic patients...
  87. pmc Skeletal muscle mitochondria in insulin resistance: differences in intermyofibrillar versus subsarcolemmal subpopulations and relationship to metabolic flexibility
    Peter Chomentowski
    Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Clin Endocrinol Metab 96:494-503. 2011
    ..Insulin resistance is accompanied by lower lipid oxidation during fasting and metabolic inflexibility. Whether these abnormalities correlate with mitochondrial content in skeletal muscle is unknown...
  88. pmc Combined risk allele score of eight type 2 diabetes genes is associated with reduced first-phase glucose-stimulated insulin secretion during hyperglycemic clamps
    Leen M 't Hart
    Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
    Diabetes 59:287-92. 2010
    ..In this study, we have investigated the combined effects of eight known beta-cell loci on insulin secretion stimulated by three different secretagogues during hyperglycemic clamps...
  89. ncbi Low plasma insulin-like growth factor-1 levels are associated with reduced insulin sensitivity and increased insulin secretion in nondiabetic subjects
    E Succurro
    Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro, Viale Europa, 88100 Catanzaro, Italy
    Nutr Metab Cardiovasc Dis 19:713-9. 2009
    ..In this cross-sectional study, we analyzed the relationship between IGF-1 levels, insulin sensitivity and secretion in 110 nondiabetic subjects with a wide range of BMI to verify this hypothesis...
  90. ncbi A Thr94Ala mutation in human liver fatty acid-binding protein contributes to reduced hepatic glycogenolysis and blunted elevation of plasma glucose levels in lipid-exposed subjects
    Martin O Weickert
    Dept of Clinical Nutrition, German Institute of Human Nutrition Potsdam Rehbruecke, Arthur Scheunert Allee 114 116, 14558 Nuthetal, Germany
    Am J Physiol Endocrinol Metab 293:E1078-84. 2007
    ....
  91. ncbi Relationship between the hyperinsulinemic-euglycaemic clamp and a new simple index assessing insulin sensitivity in overweight and obese postmenopausal women
    J P Bastard
    Service de Biochimie et Hormonologie, Hopital Tenon, APHP, 75020 Paris, France
    Diabetes Metab 33:261-8. 2007
    ..The purpose of this study was to compare assessment of insulin sensitivity from hyperinsulinemic euglycaemic (HIEG) clamp with indexes derived from fasting and oral glucose tolerance test (OGTT)...
  92. ncbi Gender differences in vascular function and insulin sensitivity in young adults
    Donald R Dengel
    School of Kinesiology, University of Minnesota, Minneapolis, MN 55455, USA
    Clin Sci (Lond) 120:153-60. 2011
    ..However, the lower EID observed in males could not be explained by artery diameter. Future studies need to continue to examine influence of gender on EID and other measures of vascular function...
  93. ncbi Decreased expression of heat shock protein 72 in skeletal muscle of patients with type 2 diabetes correlates with insulin resistance
    Istvan Kurucz
    Department of Cellular Biology, BIOREX Research and Development, Veszprem, Hungary
    Diabetes 51:1102-9. 2002
    ....
  94. pmc Hypothalamic nutrient sensing activates a forebrain-hindbrain neuronal circuit to regulate glucose production in vivo
    Carol K L Lam
    Toronto General Research Institute, University Health Network, Ontario, Canada
    Diabetes 60:107-13. 2011
    ..These studies raise the possibility that hypothalamic nutrient sensing activates a forebrain-hindbrain NMDA-dependent circuit to regulate glucose production...
  95. pmc Measuring the acute effect of insulin infusion on ATP turnover rate in human skeletal muscle using phosphorus-31 magnetic resonance saturation transfer spectroscopy
    Ee Lin Lim
    Institute of Cellular Medicine, Newcastle Magnetic Resonance Centre, Newcastle University, Newcastle upon Tyne, UK
    NMR Biomed 23:952-7. 2010
    ..03 vs baseline). The in vivo time course of insulin stimulation of skeletal muscle ATP turnover rate is not consistent with a rate limiting effect upon the initiation of insulin-stimulated glycogen synthesis...
  96. ncbi The biochemical assessment of insulin resistance
    Anwar Borai
    Centre for Clinical Science and Measurement, School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey, UK
    Ann Clin Biochem 44:324-42. 2007
    ..Insulin resistance is increasingly being assessed in clinical situations, where relatively simple markers are required. Insulin-like growth factor binding protein-1 is an emerging marker which may be useful in this context...
  97. ncbi Elevated free fatty acids attenuate the insulin-induced suppression of PDK4 gene expression in human skeletal muscle: potential role of intramuscular long-chain acyl-coenzyme A
    Kostas Tsintzas
    Centre for Integrated Systems Biology and Medicine, Institute of Clinical Research, School of Biomedical Sciences, University of Nottingham, Nottingham NG7 2UH, United Kingdom
    J Clin Endocrinol Metab 92:3967-72. 2007
    ..We investigated the effect of elevated plasma free fatty acid and insulin concentrations on PDK4 mRNA transcript and protein content and long-chain acyl-coenzyme A accumulation in human skeletal muscle...
  98. ncbi Acute elevation of plasma lipids does not affect ATP synthesis in human skeletal muscle
    A Brehm
    First Medical Department, Hanusch Hospital, Vienna, Austria
    Am J Physiol Endocrinol Metab 299:E33-8. 2010
    ..This suggests that lipid-induced insulin resistance results primarily from mechanisms decreasing glucose uptake rather than from direct interference of fatty acid metabolites with mitochondrial function...
  99. pmc Central nervous system delivery of the antipsychotic olanzapine induces hepatic insulin resistance
    Paulo J F Martins
    Obesity Research Center, University of Cincinnati, Cincinnati, Ohio, USA
    Diabetes 59:2418-25. 2010
    ..Recent evidence shows that OLZ alters insulin sensitivity independent of changes in body weight and composition. The present study addresses whether OLZ-induced insulin resistance is driven by its central actions...
  100. pmc Abnormal glucose homeostasis in skeletal muscle-specific PGC-1alpha knockout mice reveals skeletal muscle-pancreatic beta cell crosstalk
    Christoph Handschin
    Dana Farber Cancer Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 117:3463-74. 2007
    ..These data clearly illustrate a causal role for muscle PGC-1alpha in maintenance of glucose homeostasis and highlight an unexpected cytokine-mediated crosstalk between skeletal muscle and pancreatic islets...
  101. pmc Prevention of fat-induced insulin resistance by salicylate
    J K Kim
    Howard Hughes Medical Institute, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06536-8012, USA
    J Clin Invest 108:437-46. 2001
    ....

Research Grants65

  1. Ang II and Overnutrition and Insulin resistance in Cardiovascular Tissue
    JAMES RUSSELL SOWERS; Fiscal Year: 2013
    ..A better understanding of factors involved in insulin resistance should help in the development of therapeutic targets to help prevent diabetes and cardiovascular disease. ..
  2. Biology of IGFs in Bone
    Thomas L Clemens; Fiscal Year: 2013
    ..An understanding of this pathway should ultimately enable better management of diabetic veterans and conceivably lead to the design a single therapy, which can simultaneously target both osteoporosis and type 2 diabetes. ..
  3. Interactions of the RAAS and a Western Diet on Insulin Metabolic Actions
    JAMES RUSSELL SOWERS; Fiscal Year: 2013
    ..tyrosine (p) and the resultant downstream IRS-1/PI3-K/Akt signaling. ..
  4. Endoplasmic Reticulum Chaperone as a Regulator of Obesity and Diabetes
    Amy S Lee; Fiscal Year: 2010
    ..If validated, our findings may lead to new targets for therapy against obesity and type 2 diabetes in humans. ..
  5. Phospholipid-Mediated Metabolic Control in Liver and Brown Adipose Tissue
    David E Cohen; Fiscal Year: 2013
    ..The proposed studies are relevant to the mission of the NIDDK because they are expected to identify new therapeutic targets for the management of common disorders related to diabetes and obesity. ..
  6. Mechanisms and metabolic implications of LRP130 in NAFLD
    Marcus P Cooper; Fiscal Year: 2013
    ..This proposal will investigate the role of a gene called LRP130 in fatty liver disease, and may provide new ways to treat diabetes and heart disease. ..
  7. The Impact of Heat Shock Protein Expression on Inflammation and Insulin Action
    Andrea L Hevener; Fiscal Year: 2013
    ..skeletal muscle HSP72 levels, blunted JNK activity, and improved insulin sensitivity (as measured by the glucose clamp technique)...
  8. Role of Sirt1 in Regulating Glucose Homeostasis and Insulin Action in Aging
    COLETTE M KNIGHT; Fiscal Year: 2012
    ..Most significantly this study will assess how Sirtl function is altered in aging and its relationship to metabolic processes. ..
  9. Neonatal Programming of Growth and Maturation in Rhesus Monkeys
    Andrew Muir; Fiscal Year: 2013
    ..A nonhuman primate model of gestational overnutrition is a vital resource to explore the effects of weight on the health of mothers, children, and perhaps even subsequent generations. ..
  10. Phosphoprotein CDP138 regulates glucose metabolism
    ZHEN YUE JIANG; Fiscal Year: 2013
    ..Together, this project will provide valuable and novel insight into the molecular mechanisms by which CDP138 acts as a point of convergence between kinase activation and glucose transport. ..
  11. Role of Perilipin in Hepatic Steatosis and Metabolism
    Thomas A Bowman; Fiscal Year: 2013
    ..These studies will guide the design of new therapeutic targets to prevent insulin resistance and treat type 2 diabetes. ..
  12. Skeletal muscle function and mitochondrial metabolism in non-diabetic CKD
    Baback Roshanravan; Fiscal Year: 2012
    ..This understanding will ultimately identify a novel mechanism and provide an empiric basis for future large epidemiologic studies and clinical trials targeting mitochondrial dysfunction in CKD. ..
  13. Insulin Resistance in Chronic Kidney Disease
    Ian H De Boer; Fiscal Year: 2013
    ..These include the Institute for Translational Health Sciences Clinical Research Center, Nutrition and Body Composition Core, and Center for Biomedical Statistics. ..
  14. NEURAL RESPONSE TO INSULIN RESISTANCE TREATMENTS
    JASON J HASSENSTAB; Fiscal Year: 2013
    ....
  15. A short course: An organ systems approach to target the Metabolic syndrome
    Owen P McGuinness; Fiscal Year: 2012
    ..The benefit to human disease is that these trainees will now be equipped with the tools necessary to fill a void in our training programs. ..
  16. Effect of Circulating Glucose on Eating Behavior and Brain Activation in Diabetes
    RENATA D BELFORT DE AGUIAR; Fiscal Year: 2013
    ..If granted this award, Dr. Belfort De Aguiar will be able to achieve her long term career goal of becoming a clinical translational scientist in the field of neuroscience/metabolism. ..
  17. EFFECT OF HIGH FRUCTOSE CORN SYRUP FEEDING IN NONALCOHOLIC FATTY LIVER DISEASE
    Shelby A Sullivan; Fiscal Year: 2013
    ....
  18. Maternal Overweight: Consequences for Insulin Signaling in the Offspring
    Kartik Shankar; Fiscal Year: 2013
    ..Overall, these studies will elucidate important metabolic mechanisms in the offspring altered by maternal OW and high-fat diets, which may contribute to increased susceptibility to obesity. ..
  19. Effects of sleep duration and architecture on insulin sensitivity
    MADHU NAGARAJU RAO; Fiscal Year: 2013
    ..These findings may also lead to novel approaches to the treatment of insulin resistance, such as medications to alter sleep architecture or increase sleep duration. (End of Abstract) ..
  20. EFFECTS OF BARIATRIC SURGERY ON GLUCOSE HOMEOSTASIS IN TYPE-2 DIABETES
    Samuel Klein; Fiscal Year: 2012
    ..We aim to determine the effects of surgical induced weight loss on glucose metabolism as it relates to the duration of T2DM diagnosis (recent vs. longstanding). ..
  21. Bioactives in Blueberries and Insulin Resistance
    APRIL STULL; Fiscal Year: 2013
    ....
  22. Hepatic Rictor Knockout Mouse Model and the Metabolic Syndrome
    JENNIFER MARIE ROJAS; Fiscal Year: 2012
    ..The possibility of hepatic steatosis will be assessed by liver lipid quantification. ..
  23. Race - adiposity interactions regulate mechanisms determining insulin sensitivity
    Michael J Quon; Fiscal Year: 2013
    ..g., type 2 diabetes, cardiovascular disease, and cancer). ..
  24. Modulation of Insulin Action by Testosterone in Men
    Nelly Pitteloud; Fiscal Year: 2010
    ..Therefore, if low T levels are shown to play a role in the pathogenesis of insulin resistance, T may well represent an important therapeutic modality for both preventing and treating the metabolic syndrome and type 2 diabetes in men. ..
  25. DYSREGULATION OF GLUCOSE HOMEOSTASIS IN AGING
    NIR J BARZILAI; Fiscal Year: 2013
    ..Here we suggest that strategies designed to tip the balance of IGF-1 action from peripheral to brain may maximize the good effects of IGF-1 while minimizing the bad effects of IGF-1 on cancer risk, leading to healthy life-span. ..
  26. The Role of Ghrelin to Regulate Insulin Secretion in Health and Diabetes.
    Jenny Tong; Fiscal Year: 2013
    ..The knowledge gained from this project will be pivotal in determining whether the ghrelin system can be used as a drug target for the treatment of T2DM. ..
  27. MECHANISMS OF INSULIN RESISTANCE IN MAN
    Robert A Rizza; Fiscal Year: 2013
    ..In particular, this application will focus on how and why the liver releases too much glucose into the blood stream causing blood sugars to rise both before and after eating. ..
  28. Adipocyte insulin signaling and circulating adiponectin levels
    ROCIO INES PEREIRA; Fiscal Year: 2010
    ..The anticipated results of this clinical study may provide new insight into potential therapeutic targets in patients with T2DM, obesity, and MetS. ..
  29. Regulation of hepatic glucose production by Kruppel-like factor 15.
    SUSAN JESSICA GRAY; Fiscal Year: 2012
    ..Studying the mechanism by which KLF15 controls hepatic glucose production and whole-body glucose homeostasis may lead to new therapies for the treatment of metabolic disorders such as diabetes. ..
  30. Insulin Resistance and Hepatic Steatosis in Hepatitis C
    Charles F Burant; Fiscal Year: 2010
    ..abstract_text> ..
  31. UMass Mouse Metabolic Phenotyping Center
    Jason K Kim; Fiscal Year: 2013
    ....
  32. Testosterone effects on insulin sensitivity &inflammation in T2DM and obesity
    Paresh Dandona; Fiscal Year: 2012
    ..The decrease in inflammation with testosterone treatment may have relevance for atherosclerosis and cardiovascular risk. ..
  33. PKA activation enhances insulin secretion to improve glucose homeostacis in aging
    KELLY ASAKO KAIHARA; Fiscal Year: 2012
    ..The proposed studies will determine whether increased PKA signaling in ?- cells will improve ?-cell function and insulin secretory capacity in an aged population. ..
  34. Aerobic fitness, mitochondrial dysfunction, and fatty liver disease
    John P Thyfault; Fiscal Year: 2013
    ..The outcomes will also if determine if increased hepatic mitochondrial content or increased hepatic fatty acid oxidation are effective therapeutic targets to prevent NAFLD or hepatic insulin resistance. ..
  35. Glucose Insulin and Muscle Loss
    Rita R Kalyani; Fiscal Year: 2013
    ..These findings should establish a framework to evaluate the ability of existing diabetes therapies to preserve muscle and potentially identify new therapeutic targets. ..
  36. Effects of weight and insulin sensitivity on reproductive function in PCOS
    LEANNE MAREE REDMAN; Fiscal Year: 2013
    ..It is hoped that further studies will then lead to the development of evidence based treatment programs for infertility in overweight women with PCOS. ..
  37. TAUROURSODEOXYCHOLIC ACID FOR PROTEASE-INHIBITOR ASSOCIATED INSULIN RESISTANCE
    Dominic N Reeds; Fiscal Year: 2013
    ..In addition to improving clinical care of people with HIV, the findings of this study may allow development of new drugs for treatment of type 2 diabetes. ..
  38. Are Overweight Children with T1DM at Increased Risk of Cardiovascular Disease?
    Ingrid M Libman; Fiscal Year: 2010
    ..End of Abstract) ..
  39. GLUCOREGULATORY HORMONE INTERACTIONS IN DIABETES
    ROBERT STANLEY SHERWIN; Fiscal Year: 2013
    ..magnetic resonance imaging (fMRI) and magnetic resonance spectroscopy (MRS) in conjunction with the glucose clamp technique to assess the changes in brain function, neurotransmission and fuel metabolism causing the syndrome of ..
  40. A molecular basis for endotoxin mediated insulin resistance
    SOPHIE HUSSEY; Fiscal Year: 2013
    ..As such, our results could lead to the development of novel approaches for the treatment of this disease. ..
  41. Impaired adipogenesis in insulin resistance: pilot clinical and in-vitro studies
    Richard E Pratley; Fiscal Year: 2010
    ..This study tests whether differences in the growth rates of fat cells are related to the risk for diabetes in obese individuals. Results from this study could suggest new prevention / treatment strategies. ..
  42. Insulin resistance, amyloid, and memory in normal aging and preclinical AD
    JILL KATHLEEN MORRIS; Fiscal Year: 2013
    ..By investigating insulin sensitivity in subjects with or without cerebral amyloidosis, we will determine whether these factors influence insulin- mediated cognitive improvement in the very earliest stages of AD. ..
  43. Mapping Genes for Atherosclerosis and Insulin Resistance
    Jerome I Rotter; Fiscal Year: 2010
    ..Identifying the genes for CIMT and IR in this understudied population will provide new tools for risk assessment and prevention. ..
  44. Time Past Menopause, Duration of Estrogen Deficiency, and Insulin Action
    Rachael E Van Pelt; Fiscal Year: 2013
    ..Confirmation of our hypotheses will provide evidence for a benefit of E2 on insulin action (GDR) when administered early, but not late, after menopause;likely contributing to delayed onset of T2DM. ..
  45. Metabolic, and Cardiovascular Effects of GHRH in HIV Infected Patients
    STEVEN KYLE GRINSPOON; Fiscal Year: 2012
    ..We will also investigate whether the use of a secretory factor that induces the body to makes its own GH, growth hormone releasing hormone, will improve cardiovascular risk endpoints, including abdominal fat, insulin, and carotid IMT. ..
  46. Chlorolog: Production Scaling and Testing of a Fast-Acting, Ultra-stable Insulin
    BRUCE HILL FRANK; Fiscal Year: 2013
    ..The present application thus provides a logical bridge between highly promising in vitro and animal data, as generated in the Phase 1 SBIR program, and key pre-IND milestones. ..
  47. ANABOLIC ACTIONS OF INSULIN IN NEUROMUSCULAR DISEASE
    RICHARD MOXLEY; Fiscal Year: 1992
    ..The present investigations will also provide additional new information about the actions of insulin on the regulation of protein metabolism in normal man...
  48. Trypanosoma cruzi and AIDS: Role of the Adipocyte
    Herbert Tanowitz; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  49. Mechanisms Defending Fat Mass in Human after Lipectomy
    Robert Eckel; Fiscal Year: 2007
    ..abstract_text> ..
  50. Race, Lipoprotein Lipase and Obesity after Menopause
    Andrew Goldberg; Fiscal Year: 2007
    ..abstract_text> ..
  51. INSULIN RESISTANCE IN THE HIV LIPODYSTROPHY SYNDROME
    Colleen Hadigan; Fiscal Year: 2004
    ....
  52. Increased Gluconeogenesis Is One Cause of CFRD
    DANA HARDIN; Fiscal Year: 2006
    ..It is therefore expected that these studies will provide new information regarding potential causes of CF related diabetes and protein catabolism. ..
  53. The impact of PPAR-gamma and estrogen receptor crosstalk on insulin action
    Andrea Hevener; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  54. PPAR Mediated Gene Expression in HIV Patients
    Marie Gelato; Fiscal Year: 2007
    ..abstract_text> ..
  55. INSULIN RESISTANCE AND GLUCOSE METABOLISM IN PREGNANCY
    Patrick Catalano; Fiscal Year: 1991
    ..We then plan to test the value of improved treatment regimens based on the specific metabolic abnormality, to decrease maternal and perinatal morbidity...
  56. INSULIN RESISTANCE AND CALCIUM METABOLISM IN PREGNANCY
    James Sowers; Fiscal Year: 1993
    ....
  57. ALPHA AND BETA CELL FUNCTION IN HUMAN DIABETES
    John Gerich; Fiscal Year: 1993
    ..Such knowledge could lead to better selection of patients for these regimens as well as the development of means to diminsh the risks for hypoglycemia...
  58. Insulin Resistance in Tissue Specific IKKB Transgenics
    Jongsoon Lee; Fiscal Year: 2004
    ..These experiments will validate IKKbeta as a major mediator of insulin resistance and as a useful target for the discovery of new drugs to treat type 2 diabetes and insulin resistance. ..
  59. COUNTERREGULATORY FAILURE AND THE ARCUATE NUCLEUS
    Nancy Tkacs; Fiscal Year: 2001
    ....
  60. MENTORED ORIENTED RESEARCH CAREER DEVELOPMENT AWARD
    Crystal Gadegbeku; Fiscal Year: 2004
    ..In addition, this research should lay the groundwork for new treatment strategies aimed to prevent vascular disease in the high cardiovascular risk patients. ..
  61. AEROBIC CAPACITY AND METABOLIC FUNCTION IN SENIORS
    Andrew Goldberg; Fiscal Year: 1992
    ..These results will differentiate effects of primary from those of secondary aging and determine the mechanisms by which change in physical conditioning affects metabolic function...
  62. MANIPULATION OF INSULIN RESISTANCE IN TRANSGENIC MICE
    BESS MARSHALL; Fiscal Year: 1999
    ..These mice will be studied using glucose clamp and tracer techniques to evaluate the impact of an increase in glucokinase on hepatic insulin sensitivity. ..
  63. INSULIN ACTION IN CYSTIC FIBROSIS
    DANA HARDIN; Fiscal Year: 1999
    ..Dennis Bier, who will provide extensive knowledge regarding the use of stable isotopes, and use of the USDA/ARS Children's Nutrition Research Center will provide an important addition to Dr. Hardin's research training. ..
  64. Diabetes, Exercise and Postprandial Oxidative Stress
    Lauretta Quinn; Fiscal Year: 2005
    ..This study represent a novel approach to the use of aerobic exercise in the treatment of type 2 DM, providing data for future investigations and exercise training studies in this area. ..
  65. COMPARATIVE STUDIES OF INTRACELLULAR GLUCOSE METABOLISM
    Robert Henry; Fiscal Year: 1992
    ..To conduct these studies the insulin-glucose clamp technique will be employed to measure glucose uptake rates in conjunction with simultaneous computerized open ..