capillary electrophoresis

Summary

Summary: A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of proteins, nucleic acids, and carbohydrates. (Segen, Dictionary of Modern Medicine, 1992)

Top Publications

  1. ncbi In vitro selection of aptamers with affinity for neuropeptide Y using capillary electrophoresis
    Shaun D Mendonsa
    Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA
    J Am Chem Soc 127:9382-3. 2005
  2. pmc Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)
    Anne Liljander
    Department of Medicine Solna, Karolinska Institutet, SE 171 76 Stockholm, Sweden
    Malar J 8:78. 2009
  3. ncbi Quantitative metabolome profiling of colon and stomach cancer microenvironment by capillary electrophoresis time-of-flight mass spectrometry
    Akiyoshi Hirayama
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan and National Cancer Center Hospital East, Kashiwa, Chiba, Japan
    Cancer Res 69:4918-25. 2009
  4. pmc ShapeFinder: a software system for high-throughput quantitative analysis of nucleic acid reactivity information resolved by capillary electrophoresis
    Suzy M Vasa
    Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    RNA 14:1979-90. 2008
  5. ncbi Quantitative metabolome analysis using capillary electrophoresis mass spectrometry
    Tomoyoshi Soga
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997 0017, Japan
    J Proteome Res 2:488-94. 2003
  6. ncbi Metabolome analysis by capillary electrophoresis-mass spectrometry
    Maria Rowena N Monton
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997 0017, Japan
    J Chromatogr A 1168:237-46; discussion 236. 2007
  7. ncbi Simultaneous determination of anionic intermediates for Bacillus subtilis metabolic pathways by capillary electrophoresis electrospray ionization mass spectrometry
    Tomoyoshi Soga
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan
    Anal Chem 74:2233-9. 2002
  8. ncbi Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry
    T Soga
    Yokogawa Analytical Systems Inc, Tokyo, Japan
    Anal Chem 72:1236-41. 2000
  9. ncbi Structural analysis of DNA interactions with biogenic polyamines and cobalt(III)hexamine studied by Fourier transform infrared and capillary electrophoresis
    Amin Ahmed Ouameur
    Department of Chemistry Biology, University of Quebec at Trois Rivieres, C P 500, Trois Rivieres, Quebec G9A 5H7, Canada
    J Biol Chem 279:42041-54. 2004
  10. pmc Silver(I) complexes with DNA and RNA studied by Fourier transform infrared spectroscopy and capillary electrophoresis
    H Arakawa
    Department of Chemistry-Biology, , , Canada
    Biophys J 81:1580-7. 2001

Detail Information

Publications370 found, 100 shown here

  1. ncbi In vitro selection of aptamers with affinity for neuropeptide Y using capillary electrophoresis
    Shaun D Mendonsa
    Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA
    J Am Chem Soc 127:9382-3. 2005
    b>Capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX) was used to select aptamers for neuropeptide Y (NPY)...
  2. pmc Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)
    Anne Liljander
    Department of Medicine Solna, Karolinska Institutet, SE 171 76 Stockholm, Sweden
    Malar J 8:78. 2009
    ..An alternative technique for fragment analysis is capillary electrophoresis (CE) performed using automated DNA sequencers...
  3. ncbi Quantitative metabolome profiling of colon and stomach cancer microenvironment by capillary electrophoresis time-of-flight mass spectrometry
    Akiyoshi Hirayama
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan and National Cancer Center Hospital East, Kashiwa, Chiba, Japan
    Cancer Res 69:4918-25. 2009
    ..Here, we applied capillary electrophoresis time-of-flight mass spectrometry, which enables comprehensive and quantitative analysis of charged ..
  4. pmc ShapeFinder: a software system for high-throughput quantitative analysis of nucleic acid reactivity information resolved by capillary electrophoresis
    Suzy M Vasa
    Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    RNA 14:1979-90. 2008
    ..hSHAPE resolves structure-sensitive chemical modification information by high-resolution capillary electrophoresis and typically yields quantitative nucleotide flexibility information for 300-650 nucleotides (nt) per ..
  5. ncbi Quantitative metabolome analysis using capillary electrophoresis mass spectrometry
    Tomoyoshi Soga
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997 0017, Japan
    J Proteome Res 2:488-94. 2003
    A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed...
  6. ncbi Metabolome analysis by capillary electrophoresis-mass spectrometry
    Maria Rowena N Monton
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997 0017, Japan
    J Chromatogr A 1168:237-46; discussion 236. 2007
    b>Capillary electrophoresis (CE)-mass spectrometry (MS), as an analytical platform, has made significant contributions in advancing metabolomics research, if still limited up to this time...
  7. ncbi Simultaneous determination of anionic intermediates for Bacillus subtilis metabolic pathways by capillary electrophoresis electrospray ionization mass spectrometry
    Tomoyoshi Soga
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan
    Anal Chem 74:2233-9. 2002
    A method for simultaneous determination of anionic metabolites based on capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometry is described...
  8. ncbi Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry
    T Soga
    Yokogawa Analytical Systems Inc, Tokyo, Japan
    Anal Chem 72:1236-41. 2000
    A method for the determination of underivatized amino acids based on capillary electrophoresis coupled to electrospray ionization mass spectrometry (CE-ESI-MS) is described...
  9. ncbi Structural analysis of DNA interactions with biogenic polyamines and cobalt(III)hexamine studied by Fourier transform infrared and capillary electrophoresis
    Amin Ahmed Ouameur
    Department of Chemistry Biology, University of Quebec at Trois Rivieres, C P 500, Trois Rivieres, Quebec G9A 5H7, Canada
    J Biol Chem 279:42041-54. 2004
    ..and cobalt(III)hexamine on the solution structure of calf-thymus DNA were investigated using affinity capillary electrophoresis, Fourier transform infrared, and circular dichroism spectroscopic methods...
  10. pmc Silver(I) complexes with DNA and RNA studied by Fourier transform infrared spectroscopy and capillary electrophoresis
    H Arakawa
    Department of Chemistry-Biology, , , Canada
    Biophys J 81:1580-7. 2001
    ..Fourier transform infrared spectroscopy and capillary electrophoresis were used to analyze the Ag(I) binding mode, the binding constant, and the polynucleotides' structural ..
  11. ncbi N-methyl-D-glucamine improves the laser-induced fluorescence capillary electrophoresis performance in the total plasma thiols measurement
    Angelo Zinellu
    Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro 43 B, I 07100 Sassari, Italy
    Electrophoresis 24:2796-804. 2003
    We describe an ultrarapid capillary electrophoresis with laser-induced fluorescence (CE-LIF) method for total plasma thiols measurement...
  12. ncbi In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis
    Shaun D Mendonsa
    Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA
    Anal Chem 76:5387-92. 2004
    Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX...
  13. ncbi Mass spectrometry for the detection of differentially expressed proteins: a comparison of surface-enhanced laser desorption/ionization and capillary electrophoresis/mass spectrometry
    Nils V Neuhoff
    Institute of Cell and Molecular Pathology, Medizinische Hochschule Hannover, 30625 Hannover, Germany
    Rapid Commun Mass Spectrom 18:149-56. 2004
    ..mass spectroscopy based technologies, surface enhanced laser desorption ionization (SELDI-MS) and capillary electrophoresis/mass spectrometry (CE/MS), to obtain proteomic patterns of urine samples from patients suffering from ..
  14. ncbi Multilaboratory validation study of standardized multiple-locus variable-number tandem repeat analysis protocol for shiga toxin-producing Escherichia coli O157: a novel approach to normalize fragment size data between capillary electrophoresis platforms
    Eija Hyytia-Trees
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Foodborne Pathog Dis 7:129-36. 2010
    ..A set of 50 strains was tested in 10 PulseNet participating laboratories that used capillary electrophoresis instruments from two manufacturers...
  15. ncbi High-efficiency multiplex capillary electrophoresis single strand conformation polymorphism (multi-CE-SSCP) mutation screening of SCN5A: a rapid genetic approach to cardiac arrhythmia
    J Hofman-Bang
    Copenhagen Heart Arrhythmia Research Center, Statens Serum Institut, Copenhagen, Denmark
    Clin Genet 69:504-11. 2006
    ..We suggest that the method could become part of routine work-up of patients with suspicious syncope and of members of families with sudden unexplained death...
  16. ncbi Taurine determination by capillary electrophoresis with laser-induced fluorescence detection: from clinical field to quality food applications
    Angelo Zinellu
    Department of Biomedical Sciences, University of Sassari, Sassari, SS, Italy
    Amino Acids 36:35-41. 2009
    In this work we describe a new method for taurine quantification in plasma by capillary electrophoresis laser-induced fluorescence detection. Taurine is derivatized with fluorescein isothiocyanate at 100 degrees C in 20 min...
  17. ncbi Capillary electrophoresis-mass spectrometry for metabolomics
    Tomoyoshi Soga
    Institute for Advanced Biosciences, Keio University and Human Metabolome Technologies Inc, Yamagata, Japan
    Methods Mol Biol 358:129-37. 2007
    A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis-mass spectrometry (CE-MS) is described...
  18. pmc Comparison of the novel human papillomavirus 4 auto-capillary electrophoresis test with the hybrid capture 2 assay and with the PCR HPV typing set test in the detection of high-risk HPV including HPV 16 and 18 genotypes in cervical specimens
    Jin Hwa Hong
    Department of Obstetrics and Gynecology, Guro Hospital, College of Medicine, Korea University, Seoul, Korea
    J Korean Med Sci 24:579-84. 2009
    ..The aim of this study was to compare the novel human papillomavirus (HPV) detection method, the HPV 4 Auto-capillary Electrophoresis (ACE) test with the hybrid capture (HC) 2 assay for the detection of high-risk HPVs...
  19. pmc Genotyping of Bacillus anthracis strains based on automated capillary 25-loci multiple locus variable-number tandem repeats analysis
    Florigio Lista
    Army Medical Research Center, Via Santo Stefano Rotondo 4 00184 Rome Italy
    BMC Microbiol 6:33. 2006
    ..However in an emergency situation, faster genotyping and access to representative databases is necessary...
  20. ncbi Metabolomic profiling of anionic metabolites by capillary electrophoresis mass spectrometry
    Tomoyoshi Soga
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997 0052, Japan
    Anal Chem 81:6165-74. 2009
    We describe a sheath flow capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) method in the negative mode using a platinum electrospray ionization (ESI) spray needle, which allows the comprehensive analysis of anionic ..
  21. pmc Capillary electrophoresis-mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: an update of recent developments
    Harald Mischak
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    Mass Spectrom Rev 28:703-24. 2009
    ..We focus on capillary electrophoresis coupled to mass spectrometry (CE-MS), because this technique continues to show great promise...
  22. ncbi Capillary electrophoresis for the determination of major amino acids and sugars in foliage: application to the nitrogen nutrition of Sclerophyllous species
    C R Warren
    Department of Botany, University of Western Australia, Nedlands, WA 6907, Australia
    J Exp Bot 51:1147-57. 2000
    ..Two methods were developed for the analysis of underivatized sugars and amino acids by capillary electrophoresis (CE)...
  23. ncbi Capillary electrophoresis studies on the aggregation process of beta-amyloid 1-42 and 1-40 peptides
    Stefania Sabella
    Department of Pharmaceutical Chemistry, School of Pharmacy, University of Pavia, Italy
    Electrophoresis 25:3186-94. 2004
    ..the steps of beta-amyloid (Abeta) nucleation and therefore to describe this dynamic process by using capillary electrophoresis and under optimized experimental conditions is described...
  24. ncbi Multiple high-throughput analyses monitor the response of E. coli to perturbations
    Nobuyoshi Ishii
    Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997 0017, Japan
    Science 316:593-7. 2007
    ..coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations...
  25. pmc Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry
    Hideyuki Takahashi
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Exp Bot 59:3009-18. 2008
    b>Capillary electrophoresis mass spectrometry (CE/MS) was applied for the comprehensive survey of changes in the amounts of metabolites upon the shift from photoautotrophic to photomixotrophic conditions in Synechocystis sp. PCC 6803...
  26. ncbi Simultaneous detection of NPM1 and FLT3-ITD mutations by capillary electrophoresis in acute myeloid leukemia
    N I Noguera
    Department of Biopathology, University of Tor Vergata, Rome, Italy
    Leukemia 19:1479-82. 2005
    ..We developed a multiplex RT-PCR assay followed by capillary electrophoresis to simultaneously analyze NPM1 and Flt3 gene alterations (NFmPCR assay)...
  27. ncbi Capillary electrophoresis-mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery
    Walter Kolch
    The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, United Kingdom
    Mass Spectrom Rev 24:959-77. 2005
    ..Here we review the efforts that have been undertaken to date, focussing on capillary electrophoresis coupled to mass spectrometry (CE-MS)...
  28. ncbi Fundamental aspects of contactless conductivity detection for capillary electrophoresis. Part I: Frequency behavior and cell geometry
    Pavel Kuban
    Department of Chemistry, University of Basel, Basel, Switzerland
    Electrophoresis 25:3387-97. 2004
    ..The frequency setting has to be optimized for each cell design, operational amplifier, electrolyte solution and capillary...
  29. ncbi Synthesis and application of single-isomer 6-mono(alkylimidazolium)-beta-cyclodextrins as chiral selectors in chiral capillary electrophoresis
    Teng Teng Ong
    Department of Chemistry, National University of Singapore, Singapore, Singapore
    Electrophoresis 26:3839-48. 2005
    ..0-6.0) and reached a local maximum at pH 6.0. However, better resolutions were obtained with basic phosphate buffer at pH 9.6. Methanol was found to be an effective organic modifier for the resolution of Dns-amino acids by CE...
  30. ncbi Manganese species from human serum, cerebrospinal fluid analyzed by size exclusion chromatography-, capillary electrophoresis coupled to inductively coupled plasma mass spectrometry
    Bernhard Michalke
    GSF National Research Center of Environment and Health, Institute of Ecological Chemistry, 85764 Neuherberg, Germany
    J Trace Elem Med Biol 21:4-9. 2007
    ..CSF was further analyzed by CZE-ICP-DRC-MS. Several Mn-species were monitored and mostly identified. The most abundant Mn-species was Mn-citrate at a concentration of around 0.7 microg Mn L(-1)...
  31. ncbi Identification of different Ikaros cDNA transcripts in Philadelphia-positive adult acute lymphoblastic leukemia by a high-throughput capillary electrophoresis sizing method
    Ilaria Iacobucci
    Department of Hematology Oncology L and A Seràgnoli S Orsola Malpighi Hospital, University of Bologna, Bologna, Italy
    Haematologica 93:1814-21. 2008
    ..Shorter isoforms, which lack the amino-terminal domain that mediates sequence-specific DNA binding, exert a dominant negative effect and inhibit the ability of longer heterodimer partners to bind DNA...
  32. ncbi Integrative metabolomics for characterizing unknown low-abundance metabolites by capillary electrophoresis-mass spectrometry with computer simulations
    Richard Lee
    Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4M1, Canada
    Anal Chem 79:403-15. 2007
    ..In this report, an integrative strategy based on capillary electrophoresis-electrospray ionization-ion trap mass spectrometry (CE-ESI-ITMS) with computer simulations is examined ..
  33. ncbi Scaling form of viscosity at all length-scales in poly(ethylene glycol) solutions studied by fluorescence correlation spectroscopy and capillary electrophoresis
    Robert Holyst
    Institute of Physical Chemistry PAS, Kasprzaka 44 52, 01 224 Warsaw, Poland
    Phys Chem Chem Phys 11:9025-32. 2009
    We measured the viscosity of poly(ethylene glycol) (PEG 6000, 12,000, 20,000) in water using capillary electrophoresis and fluorescence correlation spectroscopy with nanoscopic probes of different diameters (from 1.7 to 114 nm)...
  34. ncbi PCR/LDR/capillary electrophoresis for detection of single-nucleotide differences between fetal and maternal DNA in maternal plasma
    Ping Yi
    Department of Obstetrics and Gynecology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, PR China
    Prenat Diagn 29:217-22. 2009
    ..We have now assessed the possibility of detecting single-nucleotide differences between fetal and maternal DNA in maternal plasma by polymerase chain reaction (PCR)/ligase detection reaction((LDR)/capillary electrophoresis.
  35. ncbi Selection of aptamers for signal transduction proteins by capillary electrophoresis
    Junie Tok
    Department of Chemistry, National University of Singapore, Republic of Singapore, Singapore
    Electrophoresis 31:2055-62. 2010
    ..No significant consensus region based on approximately 50 sequences for each protein was observed, suggesting the high efficiency of non-SELEX for the selection of numerous unique sequences with high selectivity...
  36. pmc Modified isocratic capillary electrophoresis detection of cell-free DNA in semen
    Jennifer S Chou
    Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, California, USA
    J Assist Reprod Genet 21:397-400. 2004
    ..The objectives were: i) to analyze semen for the presence of cell-free DNA and ii) to determine the association between sperm parameters and cell-free DNA...
  37. ncbi Determination of phenolic compounds in Perilla frutescens L. by capillary electrophoresis with electrochemical detection
    Youyuan Peng
    Department of Chemistry, Fudan University, Shanghai 200433, China
    J Agric Food Chem 53:8141-7. 2005
    A method based on capillary electrophoresis with electrochemical detection has been developed to analyze flavonoids and phenolic acids in Perilla frutescens L. for the first time...
  38. ncbi High-throughput single-strand conformation polymorphism analysis by automated capillary electrophoresis: robust multiplex analysis and pattern-based identification of allelic variants
    L A Larsen
    Department of Clinical Biochemistry, Statens Serum Institut, Copenhagen, Denmark
    Hum Mutat 13:318-27. 1999
    ..a method for high-throughput single-strand conformation polymorphism (SSCP) analysis using automated capillary electrophoresis. The operating temperature of a commercially available capillary electrophoresis instrument (ABI PRISM ..
  39. ncbi Comparative metabolomic study of transgenic versus conventional soybean using capillary electrophoresis-time-of-flight mass spectrometry
    Rocio Garcia-Villalba
    Faculty of Sciences, University of Granada, Fuentenueva s n, Granada, Spain
    J Chromatogr A 1195:164-73. 2008
    In this work, capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF-MS) is proposed to identify and quantify the main metabolites found in transgenic soybean and its corresponding non-transgenic parental line both grown ..
  40. ncbi Universal fluorescent multiplex PCR and capillary electrophoresis for evaluation of gene conversion between SMN1 and SMN2 in spinal muscular atrophy
    Chun Chi Wang
    School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, 100 Shih Chuan 1st Road, Kaohsiung 807, Taiwan
    Anal Bioanal Chem 397:2375-83. 2010
    We have developed a capillary electrophoresis (CE) method with universal fluorescent multiplex PCR to simultaneously detect the SMN1 and SMN2 genes in exons 7 and 8...
  41. pmc Application of capillary electrophoresis mass spectrometry and liquid chromatography multiple-step tandem electrospray mass spectrometry to profile glycoform expression during Haemophilus influenzae pathogenesis in the chinchilla model of experimental oti
    Susanna L Lundström
    Karolinska Institutet and University College of South Stockholm, Clinical Research Center, Novum, S 141 86 Huddinge, Sweden
    Infect Immun 76:3255-67. 2008
    ..Using capillary electrophoresis ESI-MS (CE-ESI-MS), we determined glycoform profiles for bacteria from direct middle ear fluid (MEF) ..
  42. pmc Multiple-locus variable-number tandem-repeat analysis for clonal identification of Vibrio parahaemolyticus isolates by using capillary electrophoresis
    Erika Harth-Chu
    Department of Vaccinology and Applied Microbiology, Helmholtz Centre of Infection Research, Braunschweig, Germany
    Appl Environ Microbiol 75:4079-88. 2009
    ..end, an advanced molecular technique was developed by applying multiplex PCR, fluorescent primers, and capillary electrophoresis, resulting in a high-resolution and high-throughput (HRHT) genotyping method...
  43. ncbi Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection
    Sheng Da Qi
    School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, China
    Anal Bioanal Chem 393:2059-66. 2009
    ..of luminally released serotonin in the feces and tissues of the rat proximal colon by means of capillary electrophoresis with laser-induced fluorescence detection...
  44. ncbi Determination of antiretroviral agents in human serum by capillary electrophoresis
    Elisabete A Pereira
    Institute of Chemistry, University of Sao Paulo, P O Box 26077, 05513 970 Sao Paulo, SP, Brazil
    J Chromatogr A 1091:169-76. 2005
    ..4%. Based on the performance characteristics, the proposed method was found suitable for the determination of zidovudine, didanosine and efavirenz in serum samples...
  45. ncbi Selection of smart aptamers by methods of kinetic capillary electrophoresis
    Andrei P Drabovich
    Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada
    Anal Chem 78:3171-8. 2006
    ..Here, we report on two kinetic capillary electrophoresis techniques applicable to the selection of smart aptamers...
  46. ncbi Plasma creatinine and creatine quantification by capillary electrophoresis diode array detector
    Angelo Zinellu
    Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro 43 B, I 07100 Sassari, Italy
    Anal Biochem 342:186-93. 2005
    ..This work describes a simple free zone capillary electrophoresis method for the simultaneous measurement of creatinine and creatine in human plasma...
  47. ncbi Improved DNA fragment length estimation in capillary electrophoresis
    Akbar Akbari
    Department of Informatics, University of Oslo, Oslo, Norway
    Electrophoresis 29:1273-85. 2008
    ..15 bp in the new method (using the estimated values for 250 and 340 bp). In the lower-molecular-weight region, excluding both fragments will improve the results by an average of 0.74 bp compared to ABI GeneMapper...
  48. pmc Accessing protein methyltransferase and demethylase enzymology using microfluidic capillary electrophoresis
    Tim J Wigle
    Center for Integrative Chemical Biology and Drug Discovery Division of Medicinal Chemistry and Natural Products, Eshelman School of Pharmacy, The University of North Carolina, CB 7363, 120 Mason Farm Road, Chapel Hill, NC 27599 7363, USA
    Chem Biol 17:695-704. 2010
    ..enable chemical exploration of these proteins, we have developed a highly quantitative microfluidic capillary electrophoresis assay to enable full mechanistic studies of these enzymes and the kinetics of their inhibition...
  49. ncbi Capillary electrophoresis is essential for microsatellite marker based detection and quantification of adulteration of Basmati rice (Oryza sativa)
    Lakshminarayana R Vemireddy
    Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500 076, Andhra Pradesh, India
    J Agric Food Chem 55:8112-7. 2007
    ..This study evaluated agarose gel electrophoresis, slab gel electrophoresis, and capillary electrophoresis techniques for their efficiency in the detection and quantification of adulteration in Basmati samples...
  50. ncbi Analysis of crude heparin by (1)H NMR, capillary electrophoresis, and strong-anion-exchange-HPLC for contamination by over sulfated chondroitin sulfate
    David A Keire
    Food and Drug Administration, Division of Pharmaceutical Analysis, 1114 Market St, Saint Louis, MO 63101, USA
    J Pharm Biomed Anal 51:921-6. 2010
    ..We examined 500MHz (1)H NMR, capillary electrophoresis (CE), and SAX-HPLC to quantify OSCS in crude heparin...
  51. pmc Gel versus capillary electrophoresis genotyping for categorizing treatment outcomes in two anti-malarial trials in Uganda
    Vinay Gupta
    Department of Medicine, University of California, San Francisco, CA 94143, USA
    Malar J 9:19. 2010
    ..The use of capillary instead of agarose gel electrophoresis for genotyping offers technical advantages, but it is unclear whether capillary electrophoresis will result in improved classification of anti-malarial treatment outcomes.
  52. ncbi High-performance liquid chromatography and capillary electrophoresis: methodological challenges for the determination of biologically relevant low-aliphatic aldehydes in human saliva
    Laura Annovazzi
    Dipartimento di Biochimica A Castellani, Universita di Pavia, Pavia, Italy
    Electrophoresis 25:1255-63. 2004
    ..whether typical chromatographic (high-performance liquid chromatography, HPLC) and/or electrophoretic (capillary electrophoresis, CE) techniques could be reliable methods for determining the levels of these analytes in human saliva, ..
  53. ncbi Translation toeprinting assays using fluorescently labeled primers and capillary electrophoresis
    Phillip S Gould
    The University of Warwick, Coventry, UK
    Biotechniques 38:397-400. 2005
    ..Furthermore, in addition to being a faster and safer method, a larger region of sequence can be analyzed with one primer in a single experiment...
  54. ncbi A quantitative assay for JAK2(V617F) mutation in myeloproliferative disorders by ARMS-PCR and capillary electrophoresis
    A M Vannucchi
    Department of Hematology, Azienda Ospedaliera Universitaria Careggi, University of Florence, Florence, Italy
    Leukemia 20:1055-60. 2006
    ..relative quantitation of mutated and normal JAK2 mRNAs using dye-labelled mutation-specific primers and capillary electrophoresis. Direct sequencing confirmed the specificity of the assay, which has a detection limit congruent with1% ..
  55. ncbi Pilot study of capillary electrophoresis coupled to mass spectrometry as a tool to define potential prostate cancer biomarkers in urine
    Dan Theodorescu
    Paul Mellon Prostate Cancer Institute, University of Virginia, Charlottesville, VA 22908, USA
    Electrophoresis 26:2797-808. 2005
    We describe the use of capillary electrophoresis (CE) coupled with mass spectrometry (MS) to identify single polypeptides and patterns of polypeptides specific for prostate cancer (CaP) in human urine...
  56. ncbi In vitro evolution of functional DNA using capillary electrophoresis
    Shaun D Mendonsa
    Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA
    J Am Chem Soc 126:20-1. 2004
    Electrophoretic selection with capillary electrophoresis (CE) is used, for the first time, to isolate functional nucleic acid sequences using SELEX (systematic evolution of ligands by exponential enrichment)...
  57. ncbi Nonradiochemical DNase I footprinting by capillary electrophoresis
    D O Wilson
    Department of Chemistry and Biochemistry, Ohio University, Athens 45701, USA
    Electrophoresis 22:1979-86. 2001
    A new application for DNase I footprinting using capillary electrophoresis (CE) has been developed in order to decrease analysis time and to eliminate the use of radiochemicals...
  58. ncbi Determination of MDMA and its metabolites in blood and urine by gas chromatography-mass spectrometry and analysis of enantiomers by capillary electrophoresis
    Nieves Pizarro
    Pharmacology Research Unit, Institut Municipal d Investigacio Medica IMIM, Barcelona, Spain
    J Anal Toxicol 26:157-65. 2002
    ..Following the same urine preparation procedure but without the derivatization step, a capillary electrophoresis (CE) method for enantiomerical resolution of compounds was developed using (2-hydroxy)propyl-beta-..
  59. pmc Diagnosis of five spinocerebellar ataxia disorders by multiplex amplification and capillary electrophoresis
    Michael O Dorschner
    Department of Medicine, Medical Genetics 357720, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195 7720, USA
    J Mol Diagn 4:108-13. 2002
    ..by polyacrylamide gel electrophoresis (PAGE) to a single multiplex amplification reaction analyzed by capillary electrophoresis (CE)...
  60. ncbi Determination of the composition of the oligosaccharide phosphate fraction of Pichia (Hansenula) holstii NRRL Y-2448 phosphomannan by capillary electrophoresis and HPLC
    Vito Ferro
    Department of Research and Development, Progen Industries Ltd, PO Box 28, Richlands BC, Qld 4077, Australia
    Carbohydr Res 337:139-46. 2002
    ..The composition of the oligosaccharide phosphate fraction was determined by capillary electrophoresis (CE) with indirect UV detection using 6 mM potassium sorbate at pH 10.3 as the background electrolyte...
  61. pmc Capillary electrophoresis with electrospray ionization mass spectrometric detection for single-cell metabolomics
    Theodore Lapainis
    Department of Chemistry and the Beckman Institute, University of Illinois, Urbana, Illinois 61801, USA
    Anal Chem 81:5858-64. 2009
    A method that enables metabolomic profiling of single cells and subcellular structures is described using capillary electrophoresis coupled to electrospray ionization time-of-flight mass spectrometry...
  62. ncbi Capillary electrophoresis in anti-cancer metallodrug research: advances and future challenges
    Christian G Hartinger
    Department of Inorganic Chemistry, University of Vienna, Vienna, Austria
    Electrophoresis 24:2023-37. 2003
    ..For many reasons, capillary electrophoresis (CE) has recently emerged as the method of choice for the separation of intact platinum metal complexes ..
  63. ncbi Congophilicity (Congo red affinity) of different beta2-microglobulin conformations characterized by dye affinity capillary electrophoresis
    N H Heegaard
    Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark
    J Chromatogr A 894:319-27. 2000
    The amyloidogenic protein beta-microglobulin was characterized by affinity capillary electrophoresis (CE)...
  64. ncbi Capillary electrophoresis with laser-induced fluorescence detection as a novel sensitive approach for the analysis of desmosines in real samples
    Laura Annovazzi
    Dipartimento di Biochimica A Castellani, Universita di Pavia, Italy
    Electrophoresis 25:683-91. 2004
    ..to decrease data variability related to the above-mentioned manipulation processes, we have developed a capillary electrophoresis approach combined with laser-induced fluorescence (CE-LIF) detection system using urine samples not ..
  65. ncbi Advances in urinary proteome analysis and biomarker discovery
    Danilo Fliser
    Mosaiques Diagnostics and Therapeutics AG, Mellendorfer Strasse 7 9, D 30625 Hannover, Germany
    J Am Soc Nephrol 18:1057-71. 2007
    ..It is predicted that proteomics will play an important role in clinical nephrology in the very near future and that this progress will require interactive dialogue and collaboration between clinicians and analytical specialists...
  66. ncbi Tau protein binds single-stranded DNA sequence specifically--the proof obtained in vitro with non-equilibrium capillary electrophoresis of equilibrium mixtures
    Svetlana M Krylova
    Department of Chemistry, York University, Toronto, Ont, Canada M3J 1P3
    FEBS Lett 579:1371-5. 2005
    ..This finding was facilitated by using two kinetic capillary electrophoresis methods: (i) non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM); (ii) affinity-..
  67. ncbi Discovery and validation of new protein biomarkers for urothelial cancer: a prospective analysis
    Dan Theodorescu
    Department of Molecular Physiology, Mellon Prostate Cancer Institute, University of Virginia, Charlottesville, VA, USA
    Lancet Oncol 7:230-40. 2006
    ..We aimed to use mass spectrometry and bioinformatics to define and validate a cancer-specific proteomic pattern...
  68. ncbi High-sensitivity quantum dot-based fluorescence resonance energy transfer bioanalysis by capillary electrophoresis
    Yong Qiang Li
    Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan, Hubei, PR China
    Biosens Bioelectron 25:1283-9. 2010
    ..In this method, capillary electrophoresis (CE) with fluorescence detection was applied...
  69. ncbi Alignment of capillary electrophoresis-mass spectrometry datasets using accurate mass information
    Ekaterina Nevedomskaya
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, 2300 RC, Leiden, Netherlands
    Anal Bioanal Chem 395:2527-33. 2009
    b>Capillary electrophoresis-mass spectrometry (CE-MS) is a powerful technique for the analysis of small soluble compounds in biological fluids...
  70. ncbi Capillary electrophoresis-SELEX selection of aptamers with affinity for HIV-1 reverse transcriptase
    Renee K Mosing
    Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA
    Anal Chem 77:6107-12. 2005
    b>Capillary electrophoresis-SELEX (CE-SELEX) was used to select ssDNA aptamers with affinity for HIV reverse transcriptase (HIVRT). A library of ssDNA was incubated with HIVRT...
  71. ncbi Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis
    Gabor Vasas
    Department of Botany, Faculty of Science, University of Debrecen, Debrecen, Hungary
    Electrophoresis 25:108-15. 2004
    ..It is recommended to use both CE techniques for the analysis of the same sample in order to confirm the results by an orthogonal approach...
  72. ncbi Determination of peptides and proteins in human urine with capillary electrophoresis-mass spectrometry, a suitable tool for the establishment of new diagnostic markers
    Stefan Wittke
    Mosaiques Diagnostics and Therapeutics AG, Feodor Lynen Strasse 5, 30625 Hannover, Germany
    J Chromatogr A 1013:173-81. 2003
    The on-line coupling of capillary electrophoresis (CE) with electrospray-time-of-flight mass spectrometry (MS) has been used to obtain patterns of peptides and proteins present in the urine of healthy human individuals...
  73. pmc Multiplex ligation-dependent probe amplification analysis on capillary electrophoresis instruments for a rapid gene copy number study
    Stéphane Jankowski
    Applied Biosystems, Foster City, CA 94404, USA
    J Biomol Tech 19:238-43. 2008
    ..MLPA polymerase chain reaction products were separated on a capillary electrophoresis platform, and the data were analyzed using GeneMapper v4...
  74. ncbi Nonequilibrium capillary electrophoresis of equilibrium mixtures: a universal tool for development of aptamers
    Maxim Berezovski
    Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada
    J Am Chem Soc 127:3165-71. 2005
    ..Here we introduce selection of aptamers by nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM). The new method has a number of advantages over conventional approaches...
  75. ncbi Discovery of biomarkers in human urine and cerebrospinal fluid by capillary electrophoresis coupled to mass spectrometry: towards new diagnostic and therapeutic approaches
    Stefan Wittke
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Electrophoresis 26:1476-87. 2005
    We report on our results using capillary electrophoresis coupled to mass spectrometry (CE-MS) to examine human bodyfluids...
  76. pmc Poly(A) leader of eukaryotic mRNA bypasses the dependence of translation on initiation factors
    Nikolay E Shirokikh
    Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia
    Proc Natl Acad Sci U S A 105:10738-43. 2008
    ....
  77. ncbi A chip-based capillary electrophoresis-contactless conductivity microsystem for fast measurements of low-explosive ionic components
    Joseph Wang
    Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces 88003, USA
    Analyst 127:719-23. 2002
    ..for separating and detecting inorganic explosive residues, based on the coupling of a micromachined capillary electrophoresis (CE) chip with a contactless conductivity detector is described...
  78. ncbi Analysis of leptin gene expression in chickens using reverse transcription polymerase chain reaction and capillary electrophoresis with laser-induced fluorescence detection
    M P Richards
    US Department of Agriculture, Livestock and Poultry Sciences Institute, Beltsville, MD 20705 2350, USA
    J Chromatogr A 853:321-35. 1999
    ..We have developed a technique using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) for the analysis of chicken leptin (261 base pairs, ..
  79. ncbi Rapid capillary electrophoresis time-of-flight mass spectrometry separations of peptides and proteins using a monoquaternarized piperazine compound (M7C4I) for capillary coatings
    Anisa Elhamili
    Department of Physical and Analytical Chemistry, Analytical Chemistry, Biomedical Center, Uppsala University, Uppsala, Sweden
    Electrophoresis 29:1619-25. 2008
    ..5 MDa could be separated. The coating showed good ability to handle these large proteins with high efficiency and retained peak shape as demonstrated by separation of IgG(1) (150 kDa) and thyroglobulin (669 kDa)...
  80. ncbi A miniaturized spatial temperature gradient capillary electrophoresis system with radiative heating and automated sample introduction for DNA mutation detection
    Zhang Run Xu
    Research Center for Analytical Sciences, Northeastern University, Shenyang, P R China
    Electrophoresis 31:3137-43. 2010
    ..3%. Utility of the system was further demonstrated by detecting K-ras gene mutations in paraffin tissue sections from two colorectal cancer patients...
  81. ncbi Simultaneous determination of allantoin, choline and L-arginine in Rhizoma Dioscoreae by capillary electrophoresis
    Lan Zhang
    Department of Chemistry, Fuzhou University, 523 Gong Ye Road, Fuzhou, Fujian 350002, China
    J Chromatogr A 1043:317-21. 2004
    A rapid, easy and reproducible capillary electrophoresis (CE) method for the simultaneous determination of allantoin, choline and arginine in Rhizoma Dioscoreae was developed first time...
  82. ncbi Detection of new amino acid sequences of alamethicins F30 by nonaqueous capillary electrophoresis-mass spectrometry
    Arndt Psurek
    University of Jena, School of Pharmacy, Department of Pharmaceutical Chemistry, Germany
    J Pept Sci 12:279-90. 2006
    ..F30) isolated from the fermentation of Trichoderma viride strain NRRL 3199 was analyzed by nonaqueous capillary electrophoresis coupled to electrospray ion-trap mass spectrometry (ESI-IT-MS) and electrospray time-of-flight mass ..
  83. ncbi Evaluation of volatile eluents and electrolytes for high-performance liquid chromatography-electrospray ionization mass spectrometry and capillary electrophoresis-electrospray ionization mass spectrometry of proteins. II. Capillary electrophoresis
    C G Huber
    Institute of Analytical Chemistry and Radiochemistry, Leopold Franzens University, Innsbruck, Austria
    J Chromatogr A 849:175-89. 1999
    Peptides and proteins were separated by capillary electrophoresis (CE) in fused-silica capillaries coated with an irreversibly adsorbed monolayer of derivatized polystyrene nanoparticles. Whereas phosphate buffer, pH 3...
  84. ncbi Determination of microcystins in environmental samples using capillary electrophoresis
    Gabor Vasas
    Department of Botany, University of Debrecen, H 4010 Debrecen, POB 14, Hungary
    J Biochem Biophys Methods 66:87-97. 2006
    ..The three microcystins could be directly determined in water bloom samples collected from Hungarian lakes and laboratory culture samples of cyanobacteria...
  85. ncbi Plasma thiols redox status by laser-induced fluorescence capillary electrophoresis
    Ciriaco Carru
    Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro 43 B, I 07100 Sassari, Italy
    Electrophoresis 25:882-9. 2004
    ..We have recently described a new laser-induced fluorescence capillary electrophoresis (CE-LIF) method to measure total plasma thiols, in which the baseline separation of cysteinylglycine, ..
  86. ncbi Analysis of amino acids and biogenic amines in breast cancer cells by capillary electrophoresis using polymer solutions containing sodium dodecyl sulfate
    Yu Yen Kao
    Department of Chemistry, National Taiwan University, 1 Section 4, Roosevelt Road, Taipei 106, Taiwan
    J Chromatogr A 1217:582-7. 2010
    ..The concentrations of Tau and Gln in the MCF-7 cells were different than those in the H184B5F5/M10 cells, respectively. Our results show the potential of this approach for cancer study...
  87. ncbi An optimized ecotilling protocol for polyploids or pooled samples using a capillary electrophoresis system
    Giovanni Cordeiro
    CRC for Sugarcane Biotechnology, Centre for Plant Conservation Genetics, Southern Cross University, Lismore NSW 2480, Australia
    Anal Biochem 355:145-7. 2006
  88. ncbi Capillary electrophoresis of biological particles: viruses, bacteria, and eukaryotic cells
    Leopold Kremser
    Institute for Analytical Chemistry, University of Vienna, Vienna, Austria
    Electrophoresis 25:2282-91. 2004
    ..Furthermore, interactions with ligands can be assessed by various modes of affinity capillary electrophoresis. Capillary zone electrophoresis has thus become a valuable tool for investigation of large ..
  89. ncbi Development of a rapid method to determine phenolic and other polar compounds in walnut by capillary electrophoresis-electrospray ionization time-of-flight mass spectrometry
    Ana M Gómez-Caravaca
    Department of Analytical Chemistry, University of Granada, c Fuentenueva s n, 18003 Granada, Spain
    J Chromatogr A 1209:238-45. 2008
    The aim of this work was to develop a capillary electrophoresis-mass spectrometry (CE-MS) method to identify and quantify phenolic and other related polar compounds in walnut samples...
  90. ncbi Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix-assisted laser desorption/ionization and electrospray-mass spectrometry detection
    Hanno Stutz
    University of Salzburg, Department of Molecular Biology, Division of Chemistry, Salzburg, Austria
    Electrophoresis 26:1254-90. 2005
    ..b>Capillary electrophoresis (CE) coupled to mass spectrometry (MS) has proven to meet these requirements...
  91. ncbi Effects of organic solvents on sample pretreatment and separation performances in capillary electrophoresis
    Carmen W Huie
    Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong
    Electrophoresis 24:1508-29. 2003
    ..and/or separation buffer for effecting sample pretreatment and/or improving separation performances in capillary electrophoresis (CE) is presented...
  92. ncbi Analysis of nucleic acid constituents by on-line capillary electrophoresis-mass spectrometry
    An V Willems
    Laboratory of Medical Biochemistry and Clinical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Belgium
    Electrophoresis 26:1221-53. 2005
    This review is focused on the capillary electrophoresis-mass spectrometric (CE-MS) analysis of nucleic acid constituents in the broadest sense, going from nucleotides and adducted nucleotides over nucleoside analogues to oligonucleotides...
  93. ncbi A rapid method for pKa determination of drugs using pressure-assisted capillary electrophoresis with photodiode array detection in drug discovery
    Yasushi Ishihama
    Analytical Research Laboratories, Eisai Co, Ltd, 5 1 3 Tokodai, Tsukuba, Ibaraki 300 2635, Japan
    J Pharm Sci 91:933-42. 2002
    We developed a rapid screening method for determination of pK(a) of candidate drugs by pressure-assisted capillary electrophoresis (CE) coupled with a photodiode array detector...
  94. ncbi Sample preconcentration by field amplification stacking for microchip-based capillary electrophoresis
    J Lichtenberg
    SAMLAB, Institute of Microtechnology, University of Neuchatel, Switzerland
    Electrophoresis 22:258-71. 2001
    ..The design features a coupled-column structure with separate stacking and capillary electrophoresis (CE) channels, showing signal enhancements of up to 65-fold for a 69 mm long stacking channel.
  95. ncbi Determination of uric acid in human urine and serum by capillary electrophoresis with chemiluminescence detection
    Shulin Zhao
    College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China
    Anal Biochem 378:127-31. 2008
    A simple and sensitive method based on capillary electrophoresis (CE) with chemiluminescence (CL) detection has been developed for the determination of uric acid (UA)...
  96. ncbi Effect of metals on herbicides-alpha-synuclein association: a possible factor in neurodegenerative disease studied by capillary electrophoresis
    Claire André
    Equipe des Sciences Séparatives et Biopharmaceutiques 2SB EA 3924, Faculte de Medecine Pharmacie, Place Saint Jacques, Besancon, France
    Electrophoresis 26:3256-64. 2005
    ..time, the association constants of alpha-synuclein with five herbicides have been calculated using a capillary electrophoresis (CE) method. In addition, the effect of a number of metals on this binding has been investigated...
  97. ncbi Recent advances in pharmaceutical applications of chiral capillary electrophoresis
    Pham Thi Thanh Ha
    Laboratory of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmaceutical Sciences, K U Leuven, Leuven, Belgium
    J Pharm Biomed Anal 41:1-11. 2006
    This review article summarizes developments and applications of chiral capillary electrophoresis (CE) in the pharmaceutical field published from January 2004 to June 2005...
  98. ncbi Capillary electrophoresis-electrospray-mass spectrometry in peptide analysis and peptidomics
    Miguel Herrero
    Institute of Industrial Fermentations, Madrid, Spain
    Electrophoresis 29:2148-60. 2008
    ....
  99. ncbi hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis in comparison to three other methods for identification of Mycobacterium species
    Anna Sajduda
    Mycobacteriology Unit, Department of Microbiology, Institute of Tropical Medicine, Nationalestraat 155, B 2000 Antwerp, Belgium
    J Microbiol Methods 80:190-7. 2010
    We developed a scheme for rapid identification of Mycobacterium species using an automated fluorescence capillary electrophoresis instrument. A 441-bp region of the hsp65 gene was examined using PCR-restriction analysis (PRA)...
  100. ncbi Effect of dextran as a run buffer additive in drug-protein binding studies using capillary electrophoresis frontal analysis
    Jesper Ostergaard
    Department of Pharmaceutics, Royal Danish School of Pharmacy, Universitetsparken 2, DK 2100 Copenhagen O, Denmark
    Anal Chem 75:207-14. 2003
    The study of drug-protein interactions by capillary electrophoresis frontal analysis requires establishment of a sufficient mobility difference between the mobility of the ligand and protein...
  101. ncbi Genotyping of K-ras codons 12 and 13 mutations in colorectal cancer by capillary electrophoresis
    Yen Ling Chen
    School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
    J Chromatogr A 1216:5147-54. 2009
    ..We established a simple and effective capillary electrophoresis (CE) method for simultaneous point mutation detection in codons 12 and 13 of K-ras gene...

Research Grants65

  1. Bioanalytical Applications of Capillary Electrophoresis
    FRANK GOMEZ; Fiscal Year: 2005
    The broad goals of this research are to further develop capillary electrophoresis (CE) as an analytical technique to study the physicochemical properties of the glycopeptide antibiotics vancomycin (Van), ristocetin (Ris), and teicoplanin ..
  2. CHOLINERGIC MECHANISMS OF REM SLEEP GENERATION
    Helen A Baghdoyan; Fiscal Year: 2013
    ..and for collecting endogenously released neurotransmitters;2) high performance liquid chromatography and capillary electrophoresis to measure changes in endogenous neurotransmitters caused by antipsychotic drugs;and 3) polygraphic ..
  3. Biophysics of Drug Interactions with Viral Episomes
    James K Bashkin; Fiscal Year: 2013
    ..The genome will be analyzed as overlapping 500 bp sequences using capillary electrophoresis and hydroxy radical footprinting...
  4. ELECTROCATALYTIC STUDIES OF TOXIC POLLUTANT ACTIVATION
    James F Rusling; Fiscal Year: 2013
    ..b>Capillary electrophoresis arrays will be used to identify specific sites of damage on key genes implicated in carcinogenesis...
  5. Multiplexed Detection of Food and Waterborne Pathogens
    Francis Barany; Fiscal Year: 2011
    ..The PCR/LDR assays will be migrated to the Cepheid GeneXpert system as well as other microfluidic devices initially using capillary electrophoresis or LDR-FRET, and subsequently Universal Array readout.
  6. Proteomics of Cell Death via 2-D Microfluidic Profiling
    DON DEVOE; Fiscal Year: 2009
    ..expertise in programmed cell death studies and bioinformatics with experience in the development of capillary electrophoresis, microfluidic, and mass spectrometry proteomic instrumentation. Dr. DeVoe (Univ...
  7. Single Cell Analysis of EGFR Activity in Human Airway Cells Exposed to Pollutants
    RYAN MATTHEW PHILLIPS; Fiscal Year: 2013
    ..the addition of a phosphate group (phosphorylation), and then measuring the rate of this process using capillary electrophoresis, which allows the separation and measurement of molecules based on differences in size and charge...
  8. Fast, High-Resolution Protein Separations using Controllable Electro-Osmotic Flow
    FRANK JAHNKE; Fiscal Year: 2010
    ..The new device also improves by a factor ot ten on the peak capacities of existing 2D chromatography, capillary electrophoresis and microfluidic devices;the cycle time is roughly comparable...
  9. FMR 1-SLS: Improving Fragile X diagnosis using amplification-free single locus ta
    STEPHEN WHITFIELD TURNER; Fiscal Year: 2013
    ..DNA fragment sizing methods are used today, either Southern Blotting or PCR followed by capillary electrophoresis, to categorize individuals as having normal, premutation or full mutation alleles...
  10. Design of Degron-based Substrates Capable of E3 Ligase-mediated Ubiquitination
    ADAM THOMAS MELVIN; Fiscal Year: 2013
    ..in myeloid leukemia-based cells such U937, THP-1, K562, NB4, Kcl-22, and KU812 using both gel and capillary electrophoresis. These assays will be utilized to generate conclusions regarding simple kinetic parameters based on ..
  11. AB3500xL DNA Analyzer for OHSU Core Facility
    Thomas J Keller; Fiscal Year: 2012
    ..ohsu.edu/xd/research/research-cores/dna- analysis/). This well-established core facility provides capillary electrophoresis sequencing services to ~100 principle investigators in 23 OHSU departments, center and institutes, and ..
  12. Detection of HIV drug resistance mutations using highly multiplexed qPCR assays
    EUGENE SPIER; Fiscal Year: 2013
    ..One critical limitation of existing Sanger sequencing methods is their lack of sensitivity. Because capillary electrophoresis sequencing calls depend on the ability to detect "mixed bases" a mutation must represent at least 20% of ..
  13. Glia classification via single cell metabolomics
    Jonathan V Sweedler; Fiscal Year: 2013
    ..When unknown metabolites are detected, we will characterize them via off-line approaches such as capillary electrophoresis hyphenated to MS...
  14. Characterizing D-amino acid-containing peptides
    Jonathan V Sweedler; Fiscal Year: 2013
    ..their component amino acids for characterization via multiple reaction monitoring and chiral amino acid capillary electrophoresis, and finally, chiral tandem mass spectrometric peptide sequencing hyphenated to in silico structure ..
  15. A self-contained wireless modular separation based sensor system
    Susan M Lunte; Fiscal Year: 2010
    ..DESCRIPTION (provided by applicant): Recent advances have shown effective neurotransmitter analysis using capillary electrophoresis (CE)...
  16. Hydrolytically stable hydrophilic CE coatings for protein characterization
    JUNIOR EMIRO SANDOVAL; Fiscal Year: 2012
    ..hydrolytic stability of current organic films that coat the inner-wall of the fused-silica tubes used in capillary electrophoresis (CE)...
  17. Capillary Electrophoresis and Protein Biophysics
    George M Whitesides; Fiscal Year: 2010
    ....
  18. Aptamers to distinguish functional modification of target proteins by affinity pr
    STEVEN SULJAK; Fiscal Year: 2009
    ..To accomplish this, affinity probe capillary electrophoresis (APCE) will be applied as a rapid method to detect fluorescently labeled aptamers that are bound to ..
  19. Study Protein-Nanomaterial Interactions and Their Impacts on Protein Activities
    Wenwan Zhong; Fiscal Year: 2011
    ..study, including flow-field flow fractionation for isolation of proteins of high affinity and capillary electrophoresis for binding affinity measurement...
  20. Urinary Polypeptide Biomarkers of IgA Nephropathy
    Jan Novak; Fiscal Year: 2010
    ..Furthermore, we have identified several potential urinary polypeptide biomarkers for IgAN by capillary electrophoresis-mass spectrometry (CE-MS) and sequenced some of these polypeptides using tandem mass spectrometry...
  21. Identifying Disease-Associated Mutations That Alter RNA Structure
    M Joan Curcio; Fiscal Year: 2010
    ..our findings using a high-throughput experimental approach we have developed that leverages multiplexed capillary electrophoresis technology (Mitra et al., 2008)...
  22. DESIGN AND USE OF METHODS FOR PEPTIDE SECRETION STUDIES
    Robert T Kennedy; Fiscal Year: 2013
    ..The method will be based on capillary liquid chromatography and capillary electrophoresis with mass spectrometry detection...
  23. Novel 5HT2C Agonist Drugs with 5HT2A Antagonist Activity for Cocaine Addiction
    Raymond G Booth; Fiscal Year: 2010
    ..Microdialysis with capillary electrophoresis/ laser-induced fluorescence will allow 15-sec temporal resolution of neurochemical changes in cocaine ..
  24. Capillary sieving electrophoresis with a cationic surfactant for size-separations
    Vladislav Dolnik; Fiscal Year: 2010
    ..The method will be ready for integration into automatable 2- dimensional capillary electrophoresis (2-D CE) of proteins...
  25. Glycolipid metabolism in single cells
    Norman J Dovichi; Fiscal Year: 2011
    ..These products are analyzed in a single cell by capillary electrophoresis with ultrasensitive laser-induced fluorescence...
  26. Nanoporous silicon membranes for protein purification
    Thomas R Gaborski; Fiscal Year: 2010
    ..risen to new levels with advances in separation tools such as two-dimensional liquid chromatography and capillary electrophoresis systems...
  27. Interleukin and the Magnocellular Neuroendocrine System
    Joan Summy Long; Fiscal Year: 2005
    ..Using immunoaffinity capillary electrophoresis with laser-induced fluorescence detection, the quantitative relation-ships among IL-1beta, OT and VP in ..
  28. High-Speed Microfluidic Assays for Measuring In Vivo Neurotransmitter Dynamics
    Michael T Bowser; Fiscal Year: 2010
    ..Introduction of online microdialysis - capillary electrophoresis (MD-CE) assays have improved temporal resolution to 10- 30 s but many issues remain...
  29. Quantitating Mitophagy in Skeletal Muscle Models
    Edgar A Arriaga; Fiscal Year: 2013
    ..rate at which mitophagy occurs) using novel technologies based on individual organelle measurements by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) and proteomic profiles...
  30. Multiplexed Single-Cell Analysis of EGFR and HER2 Activity in Gastric Cancer Cell
    ABIGAIL HALEY TURNER; Fiscal Year: 2013
    ..with a single-cell analysis system comprised of a fluorescence microscope and an exquisitely sensitive capillary electrophoresis system with laser-induced fluorescence detection...
  31. HPLC and CE of Peptides and Proteins
    Robert S Hodges; Fiscal Year: 2010
    ..in our laboratory are: (1) development of novel and improved HPLC (both analytical and preparative) and capillary electrophoresis (CE) separation protocols for peptides and proteins;and (2) utility of reversed-phase HPLC (RP-HPLC) to ..
  32. PicoSep - A Microfluidic Single Molecule Free Solution Hydrodynamic Separation Pl
    Tza Huei Wang; Fiscal Year: 2013
    ..b>Capillary electrophoresis features increased sensitivity, fast analysis times, and reduced sample consumption but with increased ..
  33. In Vivo Analysis of Adenosine Ribonucleosides by Microdialysis and Capillary Elec
    Thomas K Green; Fiscal Year: 2012
    ..The fluorescence enhancement of the derivative will allow analysis of biological samples by capillary electrophoresis with laser-induced fluorescence detection...
  34. Diagonal Capillary Electrophoresis for Phosphorylation and Sialylation Analysis
    Norman J Dovichi; Fiscal Year: 2013
    Modified Abstract. This research group recently published the first description of diagonal capillary electrophoresis. Diagonal capillary electrophoresis is a form of two-dimensional capillary electrophoresis that employs identical ..
  35. Microfluidic Selection of Aptamers
    Michael T Bowser; Fiscal Year: 2013
    ..FFE provides higher throughput and simpler fraction collection than capillary electrophoresis (CE) based selections...
  36. Rapid Generation of High Affinity Protein Sensor Elements
    HYONGSOK SOH; Fiscal Year: 2009
    ..the partition efficiency (PE) of CMACS to be 1-2 orders of magnitude higher than those achieved by capillary electrophoresis (CE)...
  37. Two-dimensional Capillary Electrophoresis - SELEX
    Norman J Dovichi; Fiscal Year: 2011
    ..of Minnesota and Krylov at York University developed a high-speed method for aptamer generation based on capillary electrophoresis. Their technology, which Bowser calls capillary electrophoresis-SELEX (CE-SELEX), is based on the ..
  38. Real-time Multiplex Single-Molecule DNA Sequencing
    Stephen Turner; Fiscal Year: 2009
    ..Current sequencing technologies based on capillary electrophoresis will likely not allow order-of-magnitude decreases in cost...
  39. Single Cell Analysis of Sphingosine Kinase Activity in Human Leukemia Stem Cells
    ALEXANDRA JAZZ DICKINSON; Fiscal Year: 2013
    ..Single-cell capillary electrophoresis (CE) will be used to separate the modified from the unmodified reporter in each cell...
  40. Quantitative Methods for Spectral and Image Data in Proteomics Research
    Timothy W Randolph; Fiscal Year: 2010
    ..vibrational spectroscopies and multidimensional separation technologies such as chromatograpy and capillary electrophoresis. This application has three primary aims: (1) developing well-defined characterizations of features with ..
  41. Long Read Next Generation Sequencing Technology Development
    Xiaochuan Zhou; Fiscal Year: 2010
    ..Generation Sanger sequencing), which will take advantage of the long time-proven Sanger sequencing and capillary electrophoresis, massively parallel sample preparation, and time-resolved capillary array imaging...
  42. Balanced, polyvalent antiglutamatergic action as a novel approach to efficacious
    Anatoly Martynyuk; Fiscal Year: 2009
    ..Microdialysis with capillary electrophoresis and laser-induced fluorescence detection will be used to elucidate the antiglutamatergic and antioxidant ..
  43. Two-dimensional Capillary Electrophoresis - SELEX
    Norman J Dovichi; Fiscal Year: 2013
    ..of Minnesota and Krylov at York University developed a high-speed method for aptamer generation based on capillary electrophoresis. Their technology, which Bowser calls capillary electrophoresis-SELEX (CE-SELEX), is based on the ..
  44. TDM & Drug Interactions in HIVinfected Substance Abusers
    Gene Morse; Fiscal Year: 2007
    ..in HIV-infected, substance abusers utilizing novel analytical approaches including HPLC, LC-MS-MS and capillary electrophoresis, 4) Develop and validate a capillary electrophoresis assay capable of enantiomeric separation to enhance ..
  45. Ultrasensitive Label-Free Flow Detector via Surface Enhanced Raman Scattering
    ZACHARY SCHULTZ; Fiscal Year: 2013
    ..3) Couple the flow detector to a capillary electrophoresis separation to analyze a complex mixture...
  46. DEVELOPMENT OF DNA MICROARRAY FABRICATION METHOD
    Maureen Cronin; Fiscal Year: 1999
    ..This was confirmed by capillary electrophoresis of synthesized products and by hybridizing with complementary oligonucleotides and PCR products...
  47. Regulation and function of Nurr1 in adult nigrostriatal dopamine neurons
    JEFFREY EELLS; Fiscal Year: 2009
    ..with gene expression technology of quantitative real-time PCR and eXpress profiling multiplex capillary electrophoresis based quantitative PCR;these experiments will demonstrate the utility of these techniques for ..
  48. Multiplexed Detection of Bioterror Agents
    Francis Barany; Fiscal Year: 2004
    ..To meet this need we propose to use ligase detection reaction (LDR) techniques combined with PCR, capillary electrophoresis, and Universal Arrays, which we have already validated in the detection of cancer gene mutations and the ..
  49. DEVICE FOR RAPID SCREENING OF PLASMA HOMOCYSTEINE
    MALONNE DAVIES; Fiscal Year: 2002
    ..The assays are based on microchip capillary electrophoresis with electrochemical detection...
  50. Capillary Electrophoresis: Bioanalytical Applications
    FRANK GOMEZ; Fiscal Year: 2003
    The broad goals of this research are to develop capillary electrophoresis (CE) as an analytical technique to study the physicochemical parameters of the antibiotics vancomycin (Van), ristocetin (Ris), and teicoplanin (Tel)...
  51. Illumina Genome Analyzer II
    David Bishop; Fiscal Year: 2009
    ..The specific aims of this proposal are: 1) to upgrade the DNAcore facility from capillary electrophoresis-based sequencing and genotyping to include massively parallel sequencing by the Illumina Genome Analyzer ..
  52. Chiral Ion Mobility Spectrometry for Rapid Separation of Enantiomeric Compounds
    Ching Wu; Fiscal Year: 2007
    ..Similar to chiral chromatography and capillary electrophoresis, the ability of chiral ion mobility spectrometry to examine enantiomeric mixtures is based upon the ..
  53. Neurochemical mechanisms of bingeing on palatable foods
    Nicole Avena; Fiscal Year: 2009
    ..to learn and apply the techniques of in situ hybridization, immunohistochemistry, radioimmuno-assay and capillary electrophoresis to identify and characterize some common neural mechanisms underlying binge eating of palatable foods ..
  54. Capillary Electrophoresis Profiling of Tears in Dry Eye
    JEAN JACOB; Fiscal Year: 2006
    ..b>Capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESI/MS) is a sensitive, relatively inexpensive method ..
  55. ON-CHIP DNA PURIFICATION/PCR FOR CANCER DIAGNOSTICS
    James Landers; Fiscal Year: 2000
    ..This methodological "bottleneck" can be solved, in part, by capillary electrophoresis (CE) and more comprehensively by "chip electrophoresis"...
  56. HIGH THROUGH-PUT AUTOMATED DNA SEQUENCING
    Judith Campbell; Fiscal Year: 2000
    ..We have chosen the ABI Prism 3700, which is based on a novel capillary electrophoresis system rather than slab gels, because of its promise of the highest through-put, with the least labor at ..
  57. Tandem TIme-of-Flight Mass Spectromer for Proteomics
    KERMIT MURRAY; Fiscal Year: 2009
    ..TOF/TOF instrument will support the study of enzyme function through the development and application of capillary electrophoresis-based approaches for studying enzyme inhibition (Gilman), research in the development of anti-Alzheimer&..
  58. A Turn Key Approach to High Throughput DNA Sequencing
    GREGORY KELLOGG; Fiscal Year: 2004
    ..results from (a) - (c) with a comparable sample prepared by ethanol precipitation and analyzed by capillary electrophoresis. This Phase I proposal is intended as part of the larger commercial effort to develop an integrated ..
  59. IN VIVO INVESTIGATIONS USING MICRODIALYSIS SAMPLING
    Craig Lunte; Fiscal Year: 2001
    ..The techniques will be based on a combination of microdialysis sampling and capillary electrophoresis. These techniques will provide methods for high throughput screening in early drug development that will ..
  60. Enhanced Capillary DNA Sequencing
    Philip Serwer; Fiscal Year: 2004
    ..A DNA sequencing ladder will be analyzed by use of several stages (analysis-stages) of conventional capillary electrophoresis. An enhancement-stage will be inserted between each neighboring pair of analysis-stages...
  61. |
    MICHAEL DUIGNAN; Fiscal Year: 1991
    High-performance capillary electrophoresis and microbore liquid chromatography offer extremely high efficiencies, ultra-small sample injection, and speed of analysis...
  62. Development of Dynamic Isoelectric Focusing for Cancer Proteomics
    Luke Tolley; Fiscal Year: 2007
    ..This greatly exceeds traditional separation methods, such as liquid chromatography (LC), capillary electrophoresis (CE), or mass spectrometry (MS), which often have peak capacities of less than 200...
  63. Bioassays and Molecular Characterization by Microchip CE
    PATRICK LIMBACH; Fiscal Year: 2007
    ..Aptamer-based assays of proteins will be used as illustrative chemical systems, and capillary electrophoresis on a multilane plastic chip with detection by laser induced fluorescence (LIF) will be the analytical ..
  64. CAPILLARY ELECTROPHORESIS-MASS SPECTROMETER (CE-MS)
    Loren Parsons; Fiscal Year: 2000
    This is a request for a Capillary Electrophoresis-Mass Spectrometry (CE- MS) instrument under the Shared Instrumentation Grant mechanism of The National Center for Research Resources...
  65. Bioanalytical CE: Mixing, Reacting, Separating, Stacking
    TIMOTHY STREIN; Fiscal Year: 2007
    ..involves the investigation of three separate approaches to improve the sensitivity and utility of capillary electrophoresis (CE) methodologies for the biochemical and clinical laboratories...