agar gel electrophoresis

Summary

Summary: Electrophoresis in which agar or agarose gel is used as the diffusion medium.

Top Publications

  1. ncbi Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media
    Jonathan R Brody
    The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Biotechniques 37:598, 600, 602. 2004
  2. ncbi Nick-forming sequences may be involved in the organization of eukaryotic chromatin into approximately 50 kbp loops
    Lorant Szekvolgyi
    Department of Biophysics and Cell Biology, University Medical School of Debrecen, P O Box 39, 4012, Debrecen, Hungary
    Histochem Cell Biol 125:63-73. 2006
  3. pmc Identification of the bacterial microflora in dairy products by temporal temperature gradient gel electrophoresis
    Jean Claude Ogier
    Institut National de la Recherche Agronomique, Unité de Recherches Laitières et Génétique Appliquée, Jouy en Josas, France
    Appl Environ Microbiol 68:3691-701. 2002
  4. ncbi Denaturing RNA electrophoresis in TAE agarose gels
    Tomas Masek
    Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Vinicna 5, 128 44, Prague, Czech Republic
    Anal Biochem 336:46-50. 2005
  5. ncbi Culture-independent methods for identifying microbial communities in cheese
    Jean Luc Jany
    Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université Européenne de Bretagne ESMISAB, Parvis Blaise Pascal, Technopôle de Brest Iroise, Plouzane, France
    Food Microbiol 25:839-48. 2008
  6. pmc Replication of mitochondrial DNA occurs by strand displacement with alternative light-strand origins, not via a strand-coupled mechanism
    Timothy A Brown
    Howard Hughes Medical Institute, Chevy Chase, Maryland 20815, USA
    Genes Dev 19:2466-76. 2005
  7. pmc Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
    Hubert Zipper
    Laboratory of Biochemistry, Institute for Interfacial Engineering, University of Stuttgart, 70569 Stuttgart, Germany
    Nucleic Acids Res 32:e103. 2004
  8. pmc Two-step total gene synthesis method
    Lei Young
    Department of Medicine, University of Sydney, Australia
    Nucleic Acids Res 32:e59. 2004
  9. ncbi Methods to study replication fork collapse in budding yeast
    Giordano Liberi
    F I R C Institute of Molecular Oncology Foundation and DSBB University of Milan, Milan, Italy
    Methods Enzymol 409:442-62. 2006
  10. ncbi Genotyping of Flavobacterium psychrophilum using PCR-RFLP analysis
    Shotaro Izumi
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Tokyo 113 8657, Japan
    Dis Aquat Organ 56:207-14. 2003

Research Grants

  1. INTERACTION OF THERMOTOLERANCE & RRNA TRANSCRIPTION
    John Subjeck; Fiscal Year: 1992
  2. Enhanced Capillary DNA Sequencing
    Philip Serwer; Fiscal Year: 2004
  3. DNA Packaging Motors
    Philip Serwer; Fiscal Year: 2005
  4. Assembly Visualized Routine Flourescence Microscopy
    Philip Serwer; Fiscal Year: 2008
  5. DNA Damage Clusters: Repair in Mammalian Cells
    Betsy Sutherland; Fiscal Year: 2004
  6. FREE SOLUTION MOBILITY AS A PROBE OF DNA ELECTROSTATICS
    Nancy Stellwagen; Fiscal Year: 2004
  7. iNOS Posttranslational Regulation in Cardiac Rejection
    GALEN PIEPER; Fiscal Year: 2009
  8. p53 target Genes in Apoptosis
    Peng Liang; Fiscal Year: 2007
  9. ROLE OF IRON-OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2007
  10. THE XIPHOPHORUS GENETIC STOCK CENTER
    RONALD WALTER; Fiscal Year: 2006

Detail Information

Publications239 found, 100 shown here

  1. ncbi Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media
    Jonathan R Brody
    The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Biotechniques 37:598, 600, 602. 2004
    ..These novel media reduce the Joule heating of the electrophoretic system and allow for easy-to-use, ultra-fast separation of DNA fragments...
  2. ncbi Nick-forming sequences may be involved in the organization of eukaryotic chromatin into approximately 50 kbp loops
    Lorant Szekvolgyi
    Department of Biophysics and Cell Biology, University Medical School of Debrecen, P O Box 39, 4012, Debrecen, Hungary
    Histochem Cell Biol 125:63-73. 2006
    ....
  3. pmc Identification of the bacterial microflora in dairy products by temporal temperature gradient gel electrophoresis
    Jean Claude Ogier
    Institut National de la Recherche Agronomique, Unité de Recherches Laitières et Génétique Appliquée, Jouy en Josas, France
    Appl Environ Microbiol 68:3691-701. 2002
    ..Subspecies, species, or groups of species of lactic acid bacteria were distinguished in dairy samples. In conclusion, TTGE was shown to distinguish bacterial species in vitro, as well as in both liquid and solid dairy products...
  4. ncbi Denaturing RNA electrophoresis in TAE agarose gels
    Tomas Masek
    Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Vinicna 5, 128 44, Prague, Czech Republic
    Anal Biochem 336:46-50. 2005
    ..Additionally, another benefit is the reduced exposure of laboratory personnel to hazardous chemicals...
  5. ncbi Culture-independent methods for identifying microbial communities in cheese
    Jean Luc Jany
    Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université Européenne de Bretagne ESMISAB, Parvis Blaise Pascal, Technopôle de Brest Iroise, Plouzane, France
    Food Microbiol 25:839-48. 2008
    ..Further, our analysis draws attention to the lack of data available on suitable DNA sequences for identifying fungal communities in cheese and proposes some potential DNA targets...
  6. pmc Replication of mitochondrial DNA occurs by strand displacement with alternative light-strand origins, not via a strand-coupled mechanism
    Timothy A Brown
    Howard Hughes Medical Institute, Chevy Chase, Maryland 20815, USA
    Genes Dev 19:2466-76. 2005
    ..These data reconcile the original displacement mode of replication with the data obtained from 2D gel analyses...
  7. pmc Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
    Hubert Zipper
    Laboratory of Biochemistry, Institute for Interfacial Engineering, University of Stuttgart, 70569 Stuttgart, Germany
    Nucleic Acids Res 32:e103. 2004
    ..These structure-property relationships help in the design of methods that use SG, in particular dsDNA quantification in solution and real-time PCR...
  8. pmc Two-step total gene synthesis method
    Lei Young
    Department of Medicine, University of Sydney, Australia
    Nucleic Acids Res 32:e59. 2004
    ..Additionally, the method is easily amenable to automation...
  9. ncbi Methods to study replication fork collapse in budding yeast
    Giordano Liberi
    F I R C Institute of Molecular Oncology Foundation and DSBB University of Milan, Milan, Italy
    Methods Enzymol 409:442-62. 2006
    ..Here we describe some experimental approaches that can be used to physically visualize the quality of replication forks in the yeast S. cerevisiae and to distinguish between stalled and collapsed forks...
  10. ncbi Genotyping of Flavobacterium psychrophilum using PCR-RFLP analysis
    Shotaro Izumi
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Tokyo 113 8657, Japan
    Dis Aquat Organ 56:207-14. 2003
    ..Genotype S was found in isolates from coho salmon (n = 5), ayu (n = 117), rainbow trout (n = 4) and other fishes (n = 40)...
  11. ncbi Pellet pestle homogenization of agarose gel slices at 45 degrees C for deoxyribonucleic acid extraction
    B T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    Anal Biochem 296:162-6. 2001
    ..Also, the number of transformants obtained using the DNA extracted with our method was at least twice that obtained using the DNA extracted with the commercial kit...
  12. pmc Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR
    Steven Giglio
    Australian Water Quality Centre, South Australian Water Corporation, Salisbury, South Australia 5108, Australia
    Nucleic Acids Res 31:e136. 2003
    ..This has implications for the design and routine application of diagnostic real-time PCR assays employing SG...
  13. ncbi A theoretical study of an empirical function for the mobility of DNA fragments in sieving matrices
    Gary W Slater
    Department of Physics, University of Ottawa, Ontario, Canada
    Electrophoresis 23:1410-6. 2002
    ..Finally, I examine how this equation may need to be modified to capture the more subtle details predicted by fundamental theories of DNA gel electrophoresis...
  14. ncbi Universal interpolating function for the dispersion coefficient of DNA fragments in sieving matrices
    Jean Francois Mercier
    Department of Physics, University of Ottawa, Ottawa, Ontario, Canada
    Electrophoresis 27:1453-61. 2006
    ..With formulas for both the mobility and the diffusion coefficient as a function of the experimental conditions one could, in principle, optimize any gel/polymer matrix-based electrophoresis system for a wide range of DNA molecular sizes...
  15. ncbi Molecular typing of a BHV-4 (bovine herpesvirus 4) field isolate
    G Donofrio
    Istituto di Malattie Infettive Profilassi e Polizia Veterinaria, Facolta di Medicina Veterinaria, Universita di Parma, Italy
    Vet Res Commun 24:411-22. 2000
    ..Taken together, these data show that PR/ 1 is a new BHV-4. We would consider that the present report provides a scheme of work for diagnosis and typing of BHV-4 isolates...
  16. ncbi A role of basic residues and the putative intercalating phenylalanine of the HMG-1 box B in DNA supercoiling and binding to four-way DNA junctions
    M Stros
    Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ 612 65 Brno, Czech Republic
    J Biol Chem 275:35699-707. 2000
    ....
  17. pmc Molecular distinction amongst varieties of mulberry using RAPD and DAMD profiles
    E Bhattacharya
    PMB Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001 UP India
    BMC Plant Biol 1:3. 2001
    ..These have been used to determine variation amongst nine varieties of Morus spp. maintained at Banthra Research Station of National Botanical Research Institute, Lucknow...
  18. pmc Short unligated sticky ends enable the observation of circularised DNA by atomic force and electron microscopies
    B Revet
    Laboratoire de microscopie cellulaire et moleculaire, CNRS URA 147, Institut Gustave Roussy, F 94805 Villejuif Cedex, France
    Nucleic Acids Res 26:2092-7. 1998
    ..Our observation provides a new powerful tool for construction of inverted dimers, and circularisation, ligation analysis or short bases sequence interaction studies...
  19. ncbi Electrophoretic mobility anomalies associated with PCR amplification of the intergenic spacer region between 16S and 23S ribosomal RNA genes of Fusobacterium necrophorum
    S K Narayanan
    Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, 1800 Denison Ave, 66506, Manhattan, KS, USA
    J Microbiol Methods 46:165-9. 2001
    ..Therefore, PCR assays relying solely on banding patterns may be unreliable, and supporting sequence analysis is essential for correct culture identification...
  20. ncbi Distribution of marine birnavirus in cultured marine fish species from Kagawa Prefecture, Japan
    T Isshiki
    Kagawa Prefectural Fisheries Experiment Station, Kagawa, Japan
    J Fish Dis 27:89-98. 2004
    ..Flatfish are suspected to be the main reservoir of MABV and might be responsible for establishing the infection cycle in aquaculture environments...
  21. ncbi Is the 16S-23S rRNA internal transcribed spacer region a good tool for use in molecular systematics and population genetics? A case study in cyanobacteria
    S L Boyer
    Department of Biology, John Carroll University, 20700 North Park Boulevard, University Heights, OH 44118, USA
    Mol Biol Evol 18:1057-69. 2001
    ..We review current knowledge of the numbers and kinds of 16S-23S ITS regions present across bacterial groups and plastids, and we discuss broad patterns congruent with higher-level systematics of prokaryotes...
  22. ncbi Rapid hemophilia A molecular diagnosis by a simple DNA sequencing procedure: identification of 14 novel mutations
    F Vidal
    Unitat de Recerca del Centre de Transfusió Banc de Teixits, Barcelona, Spain
    Thromb Haemost 85:580-3. 2001
    ..Since its implementation we have identified mutations in 45/46 hemophilia A patients, 14 of which are novel. Once the genetic defect has been identified, accurate genetic counseling is then easily performed...
  23. ncbi Alternative splicing of the imprinted candidate tumor suppressor gene ZAC regulates its antiproliferative and DNA binding activities
    B Bilanges
    Mecanismes Moleculaires des Communications Cellulaires, UPR 9023 CNRS, 141 rue de la Cardonille, F 34094 Montpellier Cedex 05, France
    Oncogene 20:1246-53. 2001
    ..Hence, these data further underline that ZAC gene is critically controlled, both at the transcriptional level through imprinting and at the functional level through alternative splicing...
  24. ncbi Occurrence of equine coital exanthema in pastured draft horses and isolation of equine herpesvirus 3 from progenital lesions
    Yoshihisa Seki
    Iwate Prefecture Central Livestock Hygiene Service Center, 390 5 Sunagome, Takizawa mura, Iwate 020 0173, Japan
    J Vet Med Sci 66:1503-8. 2004
    ..There was no epidemic of the disease in 31 mares, although 3 mares with the antibody at the pre-pastured period showed the significant increase in the titers during the pastured period...
  25. ncbi Low-cost-medium throughput Sanger dideoxy sequencing
    Kåre Lehmann Nielsen
    Department of Life Sciences, University of Aalborg, Aalborg, Denmark
    Methods Mol Biol 387:71-80. 2008
    ..Therefore, it is important to have access to a robust and affordable sequencing system. Here, we describe such a system based on the sequencing of amplified inserts of concatemer-containing clones...
  26. ncbi Genetic and biochemical analysis of common wheat cultivars lacking puroindoline a
    L Gazza
    Istituto Sperimentale per la Cerealicoltura, Via Cassia 176, 00191, Rome, Italy
    Theor Appl Genet 110:470-8. 2005
    ..The novel gene, provisionally named Pina-D1c, is the first null allele due to a point mutation that has been identified at the Pina-D1 locus...
  27. pmc Evaluation on the use of beta-lactamase and aminoglycoside modifying enzyme gene sequences as markers for the early detection of antibiotic resistance profile of Pseudomonas aeruginosa
    Victor A Doss
    Department of Biochemistry, PSG College of Arts and Science, Coimbatore, India
    Dis Markers 20:317-23. 2004
    ..The specificity (ratio of positive results obtained in both methods and the sensitivity (the minimum amount of sample DNA and the labeled probe required for the tests) were evaluated...
  28. ncbi Variation of the agr locus in Staphylococcus aureus isolates from cows with mastitis
    S Takeuchi
    Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, 4 1 1 Kenjyojima, Matsuoka, Fukui 910 1195, Japan
    Vet Microbiol 79:267-74. 2001
    ..These variations of agr locus in S. aureus bovine isolates differed from those reported by Ji et al. [Science 276 (1997) 2027]...
  29. ncbi Genetic diversity of harpins from Xanthomonas oryzae and their activity to induce hypersensitive response and disease resistance in tobacco
    Ping Li
    Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China
    Sci China C Life Sci 47:461-9. 2004
    ..All expressed hrf1, hrf2 and hrf3 proteins (Harpins) are shown to be able to induce hypersensitive reaction and TMV resistance on tobacco. Among the three proteins, Hrf2 has the highest activity while Hrf3 has the lowest activity...
  30. ncbi Sequence microdiversity at the ribosomal RNA operons of Escherichia coli pyelonephritogenic strains
    A I Anton
    Division of Microbiology, Universidad Miguel Hernandez, Campus de San Juan, Alicante, Spain
    Clin Microbiol Infect 7:345-51. 2001
    ..coli strains show significant antimicrobial resistance, high intercistronic heterogenicity (wide presence of block nucleotidic substitutions, deletions or insertions) and significantly lower virulence...
  31. ncbi Authentication of scientific human cell lines: easy-to-use DNA fingerprinting
    Wilhelm G Dirks
    DSMZ German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany
    Methods Mol Biol 290:35-50. 2005
    ..dsmz.de) constitute a low-cost but highly reliable and robust method, enabling every researcher using human cell lines to easily verify cell line identity...
  32. ncbi PCR identification of beef, sheep, goat, and pork in raw and heat-treated meat mixtures
    Miguel A Rodriguez
    Departamento de Nutrición y Bromatología III, Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
    J Food Prot 67:172-7. 2004
    ..This assay can be useful for the accurate identification of these species, avoiding mislabeling or fraudulent species substitution in meat mixtures...
  33. ncbi The bovine 5' AMPK gene family: mapping and single nucleotide polymorphism detection
    Stephanie D McKay
    Department of Veterinary Pathobiology, Texas A and M University, College Station, TX 77843 4467, USA
    Mamm Genome 14:853-8. 2003
    ..Owing to the physiological importance of this gene family, we believe that its individual genes are candidate genes for conferring resistance to diseases in cattle...
  34. ncbi Cloning and characterization of a trypsin-encoding cDNA of the human body louse Pediculus humanus
    A H Kollien
    Department of Special Zoology, Ruhr University, Bochum, Germany
    Insect Mol Biol 13:9-18. 2004
    ..After in vitro transcription and translation, the activation peptide is cleaved by chymotrypsin, a so far unreported phenomenon in trypsin activation...
  35. ncbi Use of rDNA polymorphism for identification of Heterophyidae infecting freshwater fishes
    R Dzikowski
    Department of Animal Sciences, Faculty of Agriculture, Food and Environmental Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
    Dis Aquat Organ 59:35-41. 2004
    ..By applying this method we elucidated for the first time the life cycle of Pygidiopsis genata. The phylogenetic interrelationship among the newly sequenced species of Heterophyidae is outlined...
  36. ncbi Restriction fragment length polymorphism of mitochondrial DNA and phylogenetic relationships among five species of Indian freshwater turtles
    Manoj S Rohilla
    School of Studies in Zoology, Jiwaji University, Gwalior, India
    J Appl Genet 49:167-82. 2008
    ..Further, the molecular method (PCR-RFLP) developed here is simple, rapid, reliable and reproducible; hence it can be routinely applied for species identification, essential for conservation and management of endangered chelonian species...
  37. ncbi PCR detection of Serratia spp. using primers targeting pfs and luxS genes involved in AI-2-dependent quorum sensing
    Hu Zhu
    Center for Bioengineering and Biotechnology, China University of Petroleum East China, Qingdao, 266555, People s Republic of China
    Curr Microbiol 57:326-30. 2008
    ..In this study we established a novel method to detect Serratia using quorum-sensing genes as diagnostic markers...
  38. ncbi Reproducible quantitative PCR of mitochondrial and nuclear DNA copy number using the LightCycler
    Alice Wong
    Department of Molecular Biosciences, University of California, Davis, CA, USA
    Methods Mol Biol 197:129-37. 2002
  39. pmc Telomere measurement by quantitative PCR
    Richard M Cawthon
    Department of Human Genetics, University of Utah, 15 N 2030 E, Room 2100, Salt Lake City, UT 84112, USA
    Nucleic Acids Res 30:e47. 2002
    ..This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging...
  40. ncbi Distribution and prevalence of Wolbachia in Japanese populations of Lepidoptera
    Y Tagami
    Graduate School of Biosphere Sciences, Hiroshima University, Higashi hiroshima 739 8521, Japan
    Insect Mol Biol 13:359-64. 2004
    ..The probability of detection of Wolbachia depends on the number of screened individuals of P. rapae. The results indicate that the actual number of species that are positive for Wolbachia may be higher than previously reported...
  41. pmc Detection and identification of Actinobacillus pleuropneumoniae serotypes 1, 2, and 8 by multiplex PCR
    Jennifer A Schuchert
    Center for Molecular Medicine and Infectious Diseases, Virginia Maryland Regional College of Veterinary Medicine, 1410 Prices Fork Rd, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 0342, USA
    J Clin Microbiol 42:4344-8. 2004
    ..Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments of 1.6 kb, 1.7 kb, and 970 bp from only their respective serotypes...
  42. ncbi Rapid detection of Salmonella dublin by PCR amplification of the SopE gene and its cloning
    M H Mirmomeni
    Cell and Molecular Unit, Department of Biology, Razi University, Kermanshah, Iran
    Pak J Biol Sci 11:1497-501. 2008
    ..The results showed that the obtained sequence differed in four nucleotides which resulted in two amino acid differences. The cloned SopE was submitted to the NCBI Genbank (EU399750)...
  43. ncbi Convenient determination of DNA extraction efficiency using an external DNA recovery standard and quantitative-competitive PCR
    Karen L Mumy
    Department of Microbiology, Miami University, Oxford, OH 45056, USA
    J Microbiol Methods 57:259-68. 2004
    ..Therefore, quantitative DNA studies require an estimation of DNA recovery...
  44. ncbi Generation of epitope-tagged proteins by inverse polymerase chain reaction mutagenesis
    Lucio Gama
    Division of Comparative Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
    Methods Mol Biol 182:77-83. 2002
  45. ncbi An allele-specific polymerase chain reaction assay for the differentiation of members of the Anopheles culicifacies complex
    O P Singh
    Malaria Research Centre ICMR, 22 Sham Nath Marg, Delhi 110 054, India
    J Biosci 29:275-80. 2004
    ..The assay correctly differentiates species A and D from species B, C and E. The possible use of this diagnostic assay in disease vector control programmes is discussed...
  46. ncbi Development and evaluation of a polymerase chain reaction (PCR) assay for the detection of Opisthorchis viverrini in fish
    Ammini Parvathi
    Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575 002, India
    Acta Trop 107:13-6. 2008
    ..viverrini DNA while in artificially spiked fish meat, 3 metacercariae could be detected. The results suggest that the PCR method described here is specific to O. viverrini with potential application in fish quarantining...
  47. ncbi A new amplification target for PCR-RFLP detection and identification of Chlamydiaceae species
    Vladimir V Demkin
    Laboratory of Molecular Diagnostics, Institute of Molecular Genetics RAS, Kurchatov sq 2, Moscow 123182, Russia
    Arch Microbiol 183:169-75. 2005
    ....
  48. ncbi Recombinant extracellular matrix-like proteins with repetitive elastin or collagen-like functional motifs
    Hiroyuki Kurihara
    School of Engineering, The University of Tokyo, Bunkyo ku, 113 8656 Tokyo, Japan
    Biotechnol Lett 27:665-70. 2005
    ..The resulting recombinant extracellular matrix-like proteins had the expected properties (cell adhesive ability and thermally responsive structural change) of the functional motif sequence unit used...
  49. ncbi Development of specific and rapid detection of bacterial pathogens in dairy products by PCR
    M Chotár
    Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia
    Folia Microbiol (Praha) 51:639-46. 2006
    ..coli, and 16 CFU/microL for S. aureus. This rapid, sensitive and specific diagnostic method can be performed within hours and represents an innovative diagnostic tool for the detection of milk pathogens in dairy products...
  50. ncbi Sequence-specific primed PCR (PCR-SSP) typing of HLA Class I and Class II alleles
    Klara Dalva
    Department of Hematology, Ibni Sina Hospital, Ankara University, Ankara, Turkey
    Methods Mol Med 134:51-60. 2007
    ..However, this technique is limited by the number of the samples that can be processed at one time and also by the number of the primer mixes that can be utilized...
  51. ncbi Cloning and high expression of rabbit FKBP25 in cornea
    H Kitagawa
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
    Jpn J Ophthalmol 40:133-41. 1996
    ..The higher expressions of FKBP25 and FKBP12 suggest significant roles for FKBPs in those tissues...
  52. ncbi Evidence for 5-HT1D beta but not 5-HT1D alpha receptor subtype expression in canine large coronary arteries and saphenous vein
    F Sgard
    Synthelabo Recherche, Rueil Malmaison, France
    Cardiovasc Res 31:793-9. 1996
    ..The aim of this study was to identify in the dog the 5-HT1D receptor subtype encoding mRNAs expressed in several large coronary arteries and in the saphenous vein...
  53. ncbi A novel cDNA encoding a human homologue of ribosomal protein L29
    P T Law
    Department of Biochemistry, The Chinese University of Hong Kong
    Biochim Biophys Acta 1305:105-8. 1996
    ..Northern analysis indicated that the mRNA that encodes human L29 is approx. 800 base pairs in length. An intron of hrpL29 has also been cloned and sequenced by polymerase chain reaction using human genomic DNA as the template...
  54. ncbi Type IIB mutation His-505-->Asp implicates a new segment in the control of von Willebrand factor binding to platelet glycoprotein Ib
    I Rabinowitz
    Howard Hughes Medical Institute, Jewish Hospital of St Louis, Missouri
    J Biol Chem 268:20497-501. 1993
    ..The His-505-->Asp mutation lies outside the disulfide loop affected by other type IIB vWD mutations and implicates a new segment of vWF in the regulation of platelet glycoprotein Ib binding...
  55. pmc Transgenic mice expressing high plasma concentrations of human apolipoprotein B100 and lipoprotein(a)
    M F Linton
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco 94141 9100
    J Clin Invest 92:3029-37. 1993
    ..Both the human apo-B and Lp(a) transgenic mice will be valuable resources for studying apo-B metabolism and the role of apo-B and Lp(a) in atherosclerosis...
  56. ncbi A widely applicable protocol for DNA isolation from fecal samples
    Bao Wei Zhang
    Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuan Xilu, Haidian, Beijing, PR China
    Biochem Genet 44:503-12. 2006
    ..The protocol can be tailored to the specific purpose of examining different diets of animals and can be performed with routine laboratory reagents...
  57. ncbi Two isoforms of the rat kidney EP3 receptor derived by alternative RNA splicing: intrarenal expression co-localization
    K Takeuchi
    Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan
    Biochem Biophys Res Commun 199:834-40. 1994
    ..rEP3A and rEP3B receptors are suggested to be derived by alternative RNA splicing, and both receptors are co-localized to distal tubules exerting an effect on water and electrolyte metabolism...
  58. pmc Cloning and characterization of an autonomous replication sequence from Coxiella burnetii
    M Suhan
    Department of Microbiology and Immunology, West Virginia University, Morgantown 26506 9177
    J Bacteriol 176:5233-43. 1994
    ..Two-dimensional agarose gel electrophoresis did not reveal the presence of initiating intermediates, indicating that the ARS did not initiate chromosome replication during laboratory growth of C. burnetii...
  59. ncbi Pyrosequencing-based strategies for improved allele typing of human leukocyte antigen loci
    Steven Ringquist
    Division of Immunogenetics, Department of Pediatrics, Rangos Research Center, Children s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Methods Mol Biol 373:115-34. 2007
    ....
  60. ncbi Molecular cloning, characterization, expression and functional analysis of Japanese flounder Paralichthys olivaceus Fas ligand
    Tomofumi Kurobe
    Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo 108 8477, Japan
    Dev Comp Immunol 31:687-95. 2007
    ..The cytotoxic activity was measured by MTT assay. These results indicate that fish possess a Fas ligand system...
  61. ncbi Creation of DNA overhangs by using modified DNA overhang cloning method
    Wei Li
    Laboratory of Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, People s Republic of China
    Appl Microbiol Biotechnol 75:703-9. 2007
    ..The creation of DNA overhangs by using complementary DNA oligonucleotides, T4 ligase, and RNase H is simpler and cheaper than that by using reverse transcriptase and RNase H...
  62. pmc Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria
    E A Meighen
    Department of Biochemistry, McGill University, Montreal Quebec, Canada
    J Bacteriol 174:5371-81. 1992
    ..The results indicate that there has been insertion and/or deletion of multiple 126-bp repetitive elements in the lux operons of X.luminescens during evolution...
  63. ncbi Leukocyte low density lipoprotein receptor (LDL-R) does not contribute to LDL clearance in vivo: bone marrow transplantation studies in the mouse
    S Fazio
    Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA
    J Lipid Res 38:391-400. 1997
    ..Thus, despite their large number and wide distribution, bone marrow-derived cells do not significantly influence receptor-mediated clearance of plasma LDL...
  64. ncbi The hsd loci of Mycoplasma pulmonis: organization, rearrangements and expression of genes
    R Sitaraman
    Department of Microbiology, University of Alabama at Birmingham, 35294, USA
    Mol Microbiol 26:109-20. 1997
    ....
  65. ncbi Species identification of Crassostrea and Ostrea oysters by polymerase chain reaction amplification of the 5S rRNA gene
    Ismael Cross
    Universide Cádiz, Facultad de Ciencias Mar y Ambientales, Laboratorio de Genetica, Poligono del Rio San Pedro, 11510 Puerto Real, Cadiz, Spain
    J AOAC Int 89:144-8. 2006
    ..This genetic marker can be very useful for traceability of the species, application in the management of oyster cultures, and conservation of the genetic resources of the species...
  66. ncbi The human polycystic kidney disease 2-like (PKDL) gene: exon/intron structure and evidence for a novel splicing mechanism
    L Guo
    Renal Division, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Mamm Genome 11:46-50. 2000
    ..Elucidation of the gene structure, exact location, and alternative splicing patterns of PKDL will facilitate its evaluation as a candidate gene in cystic or other genetic disorders...
  67. ncbi Detection of type III secretion system genes in animal isolates of Bordetella bronchiseptica
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, P O Box 147, Liverpool, UK
    Vet Microbiol 72:329-37. 2000
    ..bronchiseptica, it is likely that different clinical manifestations may be due to variations in gene expression or host factors, rather than the absence or presence of TTS genes...
  68. ncbi Schizosaccharomyces pombe homologue of glutathione peroxidase, which does not contain selenocysteine, is induced by several stresses and works as an antioxidant
    K Yamada
    Institute for Developmental Research, Aichi Human Service Center, 713 8 Kamiya cho, Kasugai, Aichi 480 0392, Japan
    Yeast 15:1125-32. 1999
    ..The catalase gene disruptant of S. pombe harbouring multicopy plasmid containing gpx1(+) restored the hypersensitivity to H(2)O(2) of the catalase gene disruptant. These results suggest that Gpx1 acts as a scavenger of H(2)O(2) in vivo...
  69. ncbi Molecular detection of a virus, Pilchard herpesvirus, associated with epizootics in Australasian pilchards Sardinops sagax neopilchardus
    Melanie Crockford
    Department of Fisheries Western Australia, PO Box 20, North Beach, Western Australia 6920, Australia
    Dis Aquat Organ 68:1-5. 2005
    ..Sequence analysis of amplified fragment resulting from this PCR is different from all known herpesviruses and can therefore distinguish PHV from all known viruses...
  70. ncbi Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster
    C Winstanley
    Department of Biomedical Sciences, University of Bradford, West Yorkshire
    J Med Microbiol 48:649-56. 1999
    ..solanacearum proteins. The presence of type III secretion-associated genes in B. pseudomallei pathogens suggests a possible role for type III secretion systems in the pathogenicity of this organism...
  71. ncbi cDNA cloning of canine common alpha gene and its co-expression with canine thyrotropin beta gene in baculovirus expression system
    X Yang
    Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA
    Domest Anim Endocrinol 18:379-93. 2000
    ..The expressed recombinant protein was purified from the culture medium with an affinity column that was coupled with IgG purified from the polyclonal antibodies generated against the partially purified native canine TSH...
  72. pmc Identification of variable-number tandem-repeat (VNTR) sequences in Legionella pneumophila and development of an optimized multiple-locus VNTR analysis typing scheme
    Christine Pourcel
    Institut de Genetique et Microbiologie, Bat 400, Universite Paris Sud, 91405 Orsay Cedex, France
    J Clin Microbiol 45:1190-9. 2007
    ..A database containing information from 99 L. pneumophila serogroup 1 strains and four strains of other serogroups and their MLVA profiles, which can be queried online, is available from http://bacterial-genotyping.igmors.u-psud.fr/...
  73. ncbi The ITS2 ribosomal DNA of Anopheles beklemishevi and further remarks on the phylogenetic relationships within the Anopheles maculipennis group of species (Diptera: Culicidae)
    Helge Kampen
    Institute for Medical Parasitology, University of Bonn, Sigmund Freud Str 25, 53105, Bonn, Germany
    Parasitol Res 97:118-28. 2005
    ..It is hypothesized that during the evolution An beklemishevi separated first from the common ancestor of the palaearctic species, which had presumably made its way from the Nearctic to the Palaearctic...
  74. ncbi Real-time detection of allele-specific polymerase chain reaction products by automated ultra-thin-layer agarose gel electrophoresis
    A Guttman
    Genetic BioSystems Inc, San Diego, CA 92121, USA
    J Chromatogr A 828:481-7. 1998
    ....
  75. ncbi Partial sequencing and tissue distribution of the canine isoforms of steroid 5alpha-reductase type I and type II
    P N Span
    Department of Chemical Endocrinology, University Medical Center Sint Radboud, Nijmegen, The Netherlands
    Prostate 44:233-9. 2000
    ..The dog is regarded to be a valid model to test the effects of 5alpha-reductase inhibitors on prostatic growth. However, limited information is available on the characteristics or even existence of 5alpha-reductase isozymes in this species...
  76. ncbi Application of the PCR-RFLP method for the rapid differentiation of Spodoptera exigua nucleopolyhedrovirus genotypes
    Rosa Murillo
    Departamento de Producción Agraria, Universidad Publica de Navarra, Pamplona 31006, Spain
    J Virol Methods 135:1-8. 2006
    ..This method constitutes a useful tool for processing large number of environmental samples and could be used to address environmental biosafety concerns...
  77. ncbi Molecular characterization of PDGFR-alpha/PDGF-A and c-KIT/SCF in gliosarcomas
    Rui M Reis
    Life and Health Sciences Research Institute ICVS, School of Health Sciences, University of Minho, Braga, Portugal
    Cell Oncol 27:319-26. 2005
    ..Nevertheless, the presence of a PDGFR-a/PDGFA and c-Kit/SCF autocrine/paracrine stimulation loop in a proportion of cases, supports the potential role of specific tyrosine kinase inhibitors in the treatment of gliosarcomas...
  78. ncbi Generation of DNA double-strand breaks by two independent enzymatic activities in nuclear extracts
    Miguel G Blanco
    Departamento de Bioquimica e Bioloxia Molecular, Facultade de Bioloxia, Universidade de Santiago de Compostela, Galicia 15782, Spain
    J Mol Biol 351:995-1006. 2005
    ..The tissue distribution of both DSB-producing activities was also different. The in vitro system used in the present work may be a useful tool for studying the formation of DSB and for investigation of the mechanisms of DNA repair...
  79. ncbi Lipoprotein-X in patients with cirrhosis: its relationship to cholestasis and hypercholesterolemia
    P Soros
    Abteilung Gastroenterologie und Hepatologie, Medizinische Hochschule Hannover, Hannover, Germany
    Hepatology 28:1199-205. 1998
    ..The detection of Lp-X and the quantification of -, beta-, and pre-beta-cholesterol was based on agar gel electrophoresis and polyanion precipitation...
  80. ncbi The primary structure and enzymic properties of porcine prochymosin and chymosin
    G Houen
    Department of Autoimmunology, Statens Seruminstitut, Copenhagen, Denmark
    Int J Biochem Cell Biol 28:667-75. 1996
    ..b>Agar gel electrophoresis at pH 5...
  81. ncbi Secretion of total pepsin and pepsin 1 in healthy volunteers in response to pentagastrin and to insulin-induced hypoglycaemia
    Norman Bryson Roberts
    Department of Clinical Biochemistry and Metabolic Medicine, Royal Liverpool and Broadgreen University Hospital, Liverpool, UK
    Scand J Gastroenterol 42:555-61. 2007
    ..The purpose of this study was to determine the pepsin 1 content in relation to the total pepsin of human gastric juice in healthy volunteers and the effect of different stimuli on secretion...
  82. ncbi The iron chelator pyridoxal isonicotinoyl hydrazone (PIH) protects plasmid pUC-18 DNA against *OH-mediated strand breaks
    M Hermes-Lima
    Departamento de Biologia Celular, Universidade de Brasilia, DF, Brazil
    Free Radic Biol Med 25:875-80. 1998
    ..damage was quantified by determining the diminution of supercoiled DNA forms after oxidative attack using agar gel electrophoresis. Micromolar amounts of PIH (20-30 microM) were able to half-protect DNA from iron (1-7...
  83. ncbi Review article: human pepsins - their multiplicity, function and role in reflux disease
    N B Roberts
    Department of Clinical Biochemistry and Metabolic Medicine, Royal Liverpool University Hospital, Liverpool, UK
    Aliment Pharmacol Ther 24:2-9. 2006
    ..At least seven zones of activity can be visualized by agar gel electrophoresis and a similar number of separate proteins resolved by high performance ion exchange chromatography...
  84. pmc Expression of human liver arginase in Escherichia coli. Purification and properties of the product
    M Ikemoto
    College of Medical Technology, Kyoto University, Japan
    Biochem J 270:697-703. 1990
    ..It was thus concluded that, in Nature, the liver and erythrocyte arginases are identical proteins...
  85. ncbi Toxicities induced in cultured cells exposed to zearalenone: apoptosis or mutagenesis?
    Imen Ayed-Boussema
    Laboratory of Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Monastir University, Rue Avicenne, 5019 Monastir, Tunisia
    J Biochem Mol Toxicol 21:136-44. 2007
    ..correlated to cell cycle perturbation-induced DNA fragmentation, resulting in DNA-laddering patterns on agar gel electrophoresis. This observation is consistent with apoptosis, which was confirmed by induction of apoptotic bodies...
  86. ncbi PCR-based ribotyping of Staphylococcus aureus
    A M Oliveira
    Disciplina de Moléstias Infecciosas e Parasitárias, Departamento de Clinica Medica, Faculdade de Ciencias Medicas, Universidade Estadual de Campinas, Campinas, SP, Brasil
    Braz J Med Biol Res 35:175-80. 2002
    ..pairs were tested to determine the size of amplicons produced and to obtain better discrimination with agar gel electrophoresis and ethidium bromide staining...
  87. ncbi Growth performance, meat quality and activities of glycolytic enzymes in the blood and muscle tissue of calves infected with Sarcocystis cruzi
    A Daugschies
    Institut für Parasitologie, Tierarztliche Hochschule Hannover, Germany
    Vet Parasitol 88:7-16. 2000
    ..Although these enzymes are also synthesized by the parasite, the contribution of parasite-derived enzymes to the observed changes of enzyme patterns remains in question...
  88. ncbi Hemoglobinopathy York [beta146 (HC3) His==>Pro]: first report of a family history
    E Misgeld
    Klinik für Hämatologie, Onkologie und klinische Immunologie, Heinrich Heinrich Heine Universität, Dusseldorf, Germany
    Ann Hematol 80:365-7. 2001
    ..We have encountered a new case of Hb York, which was detected by agar gel electrophoresis at pH 6.0. Analysis of DNA sequences revealed a CAC-->CCC mutation in codon 146...
  89. ncbi Progressive central and peripheral demyelinating disease of adult onset in a Norwegian family
    K Hagen
    Department of Neurology, Trondheim University Hospital, Norway
    Arch Neurol 55:1467-72. 1998
    ..To describe the clinical features of a Norwegian family with a combined central and peripheral demyelinating disease...
  90. ncbi Activation of PI3-K/Akt pathway for thermal preconditioning to protect cultured cerebellar granule neurons against low potassium-induced apoptosis
    Lin Cao
    Department of Pharmacology, Zhongshan Medical School, Sun Yat Sen University, Guangzhou 510080, China
    Acta Pharmacol Sin 28:173-9. 2007
    ....
  91. ncbi [Growth differentiation factor-9 gene expression in in vitro cultured oocytes in mice]
    Yu Hong Peng
    Department of Obstetrics and Gynecology, Wuhan General Hospital of Guangzhou Command, Wuhan 430070, China
    Nan Fang Yi Ke Da Xue Xue Bao 26:1341-5. 2006
    ..To explore the relation between oocyte maturation and growth differentiation factor-9 (GDF-9) gene expression...
  92. ncbi LDL cholesterol estimation using the Anandaraja's and Friedewald's formulas in schizophrenic patients treated with antipsychotic drugs
    Eduardo Paz
    Hospital Psiquíatrico de Conxo, Complejo Hospitalario Universitario, Santiago de Compostela, Spain
    Clin Biochem 41:1002-7. 2008
    ..Int J Cardiol 2005; 102: 117), and the aim of our study was its evaluation in schizophrenic patients treated with antipsychotic drugs...
  93. ncbi The SCID mouse as a model for multiple myeloma
    E J Ahsmann
    University Hospital Utrecht, The Netherlands
    Br J Haematol 89:319-27. 1995
    ..and their monoclonality biochemically characterized, using a sensitive immunoblotting technique after agar gel electrophoresis. Successful transplantation of bone marrow derived-tumour cells in SCID mice was obtained with BMCC of ..
  94. ncbi [Effect of arsenic trioxide on apoptosis in lymphoblastoid Raji cell line with relation to expression of mcl-1 gene]
    Zhi hui Li
    Department of Hematology, Yunnan Provincial Second People Hospital, Kunmin, Yunnan Province, China
    Zhongguo Shi Yan Xue Ye Xue Za Zhi 17:597-601. 2009
    ..with different concentrations of As(2)O(3), the classical DNA ladder of cell apoptosis was detected by agar gel electrophoresis, the changes of mitochondrial membrane potential in Raji cells were assayed by confocal laser scanning ..
  95. ncbi Ethanol-induced cell death in cultured rat astroglia
    A Holownia
    Laboratoire de Biochimie et Nutrition, Faculte de Medecine, Brest, France
    Neurotoxicol Teratol 19:141-6. 1997
    ..with flow cytometry and with the random oligonucleotide primed synthesis assay but neither following agar gel electrophoresis nor in UV microscopy of cell nuclei...
  96. ncbi [Interest of new molecular typing method in the study of hospital transmitted Staphylococcus aureus population]
    S Vedy
    Laboratoire de Biologie Medicale, HIA Laveran, boulevard A Laveran, 13998 Marseille, France
    Pathol Biol (Paris) 55:382-9. 2007
    ..To determinate the origin of acquired S. aureus among hospitalised patients and to evaluate the transmission of strains between health care workers and hopistalised patients...
  97. ncbi [Identification of E-4031-sensitive potassium current component in murine P19 embryonic carcinoma cell line differentiated in cardiomyocytes]
    H V Sotkis
    Fiziol Zh 52:49-61. 2006
    ..Cardiac-differentiated P19 cells might also be useful for studying pharmacological modulation of I(Kr), which is recognized target for cardiotoxic side effects of numerous drugs...
  98. ncbi Absence of mycoplasmal gene in malignant mammalian cells transformed by chronic persistent infection of mycoplasmas
    B Zhang
    Department of Transfusion Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    Proc Soc Exp Biol Med 218:83-9. 1998
    ....
  99. ncbi The novel cytotoxic sponge metabolite peloruside A, structurally similar to bryostatin-1, has unique bioactivity independent of protein kinase C
    K A Hood
    School of Biological Sciences, Victoria University of Wellington, New Zealand
    Anticancer Drug Des 16:155-66. 2001
    ..In summary, although structurally similar to bryostatin, peloruside is a potent inhibitor of cell proliferation, has apoptosis-inducing properties and has a unique mode of action independent of PKC...
  100. ncbi Bovine leukocyte adhesion deficiency in Danish Holstein-Friesian cattle. I. PCR screening and allele frequency estimation
    C B Jørgensen
    Department of Animal Science and Animal Health, Royal Veterinary and Agricultural University, Copenhagen, Denmark
    Acta Vet Scand 34:231-6. 1993
    ..Chromosome analysis showed that this bull was a blood chimaera. Estimation of the BLAD allele frequency upon the PCR test results showed that around 450 Danish calves born in 1991 might have been affected with the recessive disorder...
  101. ncbi [Genetic study on 27 children with beta-thalassemia major and their parents in Sichuan area]
    Xi Hong Li
    Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China
    Sichuan Da Xue Xue Bao Yi Xue Ban 35:388-90. 2004
    ....

Research Grants59

  1. INTERACTION OF THERMOTOLERANCE & RRNA TRANSCRIPTION
    John Subjeck; Fiscal Year: 1992
    ..Techniques: In-vivo labeling, polyacrylamide and agar gel electrophoresis, density gradient centrifugation, cell survival, in- vivo/in-vitro tumor systems, cell fractionation, in-..
  2. Enhanced Capillary DNA Sequencing
    Philip Serwer; Fiscal Year: 2004
    ..Each adaptation will help solve a current genomic problem, for example, either closing of a partially assembled genome sequence or performing high accuracy analysis of mutations. ..
  3. DNA Packaging Motors
    Philip Serwer; Fiscal Year: 2005
    ..Analysis of bacteriophage DNA packaging motors is expected to provide a basis for understanding the role of motors in disease, especially disease caused by a virus that has a DNA packaging motor. ..
  4. Assembly Visualized Routine Flourescence Microscopy
    Philip Serwer; Fiscal Year: 2008
    ..Applications are anticipated in the analysis of the assembly of disease-causing viruses and determining pathways of oncogenesis. ..
  5. DNA Damage Clusters: Repair in Mammalian Cells
    Betsy Sutherland; Fiscal Year: 2004
    ..Further, knowledge of conditions affecting formation of clustered damages, and cellular paths for dealing with them could allow the design of more effective radiation therapies. ..
  6. FREE SOLUTION MOBILITY AS A PROBE OF DNA ELECTROSTATICS
    Nancy Stellwagen; Fiscal Year: 2004
    ..Further work entails an examination of the interaction between DNA and commonly used buffers in electrophoresis experiments. ..
  7. iNOS Posttranslational Regulation in Cardiac Rejection
    GALEN PIEPER; Fiscal Year: 2009
    ..A better understanding of this molecular process may lead to better strategies to prevent injury cardiac cells in these patients. ..
  8. p53 target Genes in Apoptosis
    Peng Liang; Fiscal Year: 2007
    ..abstract_text> ..
  9. ROLE OF IRON-OXO CLUSTERS IN FATTY ACID DESATURATION
    Brian Fox; Fiscal Year: 2007
    ..The potential of using related molecules to inactivate other position-specific desaturases will be determined. ..
  10. THE XIPHOPHORUS GENETIC STOCK CENTER
    RONALD WALTER; Fiscal Year: 2006
    ..abstract_text> ..
  11. Role of iNOS and CAT-2 in defense against CP infection
    Jody Gookin; Fiscal Year: 2006
    ..parvum infection. The PI is presently in the 3rd year of a 5-year K08 from NIDDK. These studies are anticipated to generate key preliminary data for the PI's first R01 application ..
  12. NO& Epithelial Repair in Cryptosporidiosis
    Jody Gookin; Fiscal Year: 2006
    ..abstract_text> ..
  13. FUNCTION AND MECHANISM OF DNA TOPOISOMERASE
    Tao Shih Hsieh; Fiscal Year: 2010
    ..This will also allow us to examine the mechanistic basis of a number of anti-cancer drugs that affect the equilibrium of DNA cleavage and religation. ..
  14. Roles of Nemo-like kinase in CaP and its effects on androgen receptor signaling
    Eva Corey; Fiscal Year: 2010
    ..By studying the ability of nemo-like kinase to kill even prostate-cancer cells that are resistant to other treatments, we hope to point the way to new and promising therapeutic approaches for advanced prostate cancer. ..
  15. Training of Claflin Minorities at the USC Cancer Center
    Omar Bagasra; Fiscal Year: 2005
    ..The PIs of these projects will be Dr. Omar Bagasra of Claflin University, and Dr. Kim E. Creek of the SCCC. ..
  16. THE ROLE OF PLACENTAL LACTOGEN IN FETAL DEVELOPMENT
    Michael Freemark; Fiscal Year: 2007
    ..The results of our studies should provide new insist into the roles of pituitary and placental hormones in adipose development and function. ..
  17. Interleukin 24 in Epidermal Function
    Peng Liang; Fiscal Year: 2007
    ..Specific Aim 2: To determine the role of IL-24 in wound healing using mouse skin excision wound model and IL-24 antagonists. [unreadable] [unreadable]..
  18. Detection of Topoisomerase II Mediated DNA Damage in Stroke
    VLADIMIR DIDENKO; Fiscal Year: 2007
    ..It will also have a broad applicability in all fields of biomedicine where detection of early apoptosis is important. [unreadable] [unreadable] [unreadable]..
  19. Hormonal and Genetic Influences in a Mouse Model of Sjogrens Syndrome
    Ana Maria Azzarolo; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  20. Aberrant DNA Methylation as a Biomarker of HSIL in Liquid-based Pap Tests
    KAREN GUSTAFSON; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  21. Genomic Integrity, Aging, and Longevity
    Richard Cawthon; Fiscal Year: 2008
    ..Therefore, this research is likely to speed the development of medical interventions that slow aging and extend the duration of healthy adult life. [unreadable] [unreadable] [unreadable]..
  22. Calpain and Calpastatin Regulation of Reperfusion Injury
    JODIE DUFFY; Fiscal Year: 2008
    ..Determining calpain and calpastatin regulation of these pathways identifies therapeutic targets to reduce postoperative myocardial dysfunction in pediatric patients. ..
  23. Semi-artificial nanomachines for detection of DNA damage and apoptosis
    VLADIMIR DIDENKO; Fiscal Year: 2008
    ..abstract_text> ..
  24. ET and PAF Synergism in intrauterine growth restriction
    LARRY THAETE; Fiscal Year: 2008
    ..Our goal is to assess the specific role of ET-1, as well as its interaction with PAF, in the pathophysiology of I/R-induced IUGR. ..
  25. Choroid plexus & antidepressants: genomics & proteomics
    SAMUEL SATHYANESAN; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  26. Identifying human genes required by tick-borne pathogens
    Ulrike Munderloh; Fiscal Year: 2007
    ..We anticipate that the interaction of the human host cells with these highly specialized intracellular pathogens will shed light,on the nature of some unidentified human genes. [unreadable] [unreadable] [unreadable]..
  27. Designing Spider Silk Proteins as Novel Biomaterials
    Randolph Lewis; Fiscal Year: 2009
    ..Possible applications of spider silk range from artificial ligaments and tendons to bandages for burns to composite materials for multiple applications. ..
  28. Genetics of Lipoproteins in Afro-Caribbean Families
    Iva Miljkovic Gacic; Fiscal Year: 2007
    ..Our long-term goal is to dissect the genetic architecture of traditional and nuclear magnetic spectroscopy (NMR) measured lipoprotein phenotypes in extended, multi-generational families of African heritage. [unreadable] [unreadable]..
  29. Mycobacterium avium subspecies in Crohn's Disease
    Saleh Naser; Fiscal Year: 2005
    ..Ultimately, the outcome of this study will go a long way toward clearing up the MAP debate. ..
  30. Functional Proteogenomics of Mitochondria
    Peter Oefner; Fiscal Year: 2005
    ..The concordance of protein data with those obtained by means of human mRNA expression arrays and the functional genomic findings from yeast will be assessed. ..
  31. IMPACT OF ENDOTHELIN IN INTRAUTERINE GROWTH RESTRICTION
    LARRY THAETE; Fiscal Year: 2005
    ....
  32. Defining Critical Parameters of Mouse Cloning
    Robert Lanza; Fiscal Year: 2001
    ..They should also validate the choice of the mouse as a model system. The proposed research is expected to improve the efficiency of this technique, although this is not the primary objective. ..
  33. DNA DAMAGE QUANTITATION BY SINGLE MOLECULE LASER SIZING
    John Sutherland; Fiscal Year: 2002
    ....
  34. MITOCHONDRIAL DNA ANALYSIS IN CYCLIC VOMITING SYNDROME
    Richard Boles; Fiscal Year: 2002
    ..An extensive amount of clinical and laboratory data will be collected in all patients, allowing for the comparison of CVS sufferers with and without mtDNA mutations. ..
  35. DNA DAMAGE AND MUTAGENICITY OF OCHRATOXIN A
    Richard Manderville; Fiscal Year: 2002
    ....
  36. ENDOTHELIN AND INTRAUTERINE GROWTH RESTRICTION
    LARRY THAETE; Fiscal Year: 2002
    ..Cellular localization of ET, ET receptors, and NOS isoforms will be performed with immunocytochemistry. ..
  37. ENHANCED DEVELOPMENT OF THE XIPHOPHORUS MODEL SYSTEM
    RONALD WALTER; Fiscal Year: 2001
    ....
  38. CHIP BASED QTL MAPPING IN ARABIDOPSIS
    Peter Oefner; Fiscal Year: 2001
    ..It is anticipated that experience gained on the mapping of QTLs in Arabidopsis will be very valuable for similar efforts in other eukaryotes, including human. ..
  39. STREAMLINING DIFFERENTIAL DISPLAY TECHNOLOGY
    Peng Liang; Fiscal Year: 2001
    ..5) Using oligo-capping technique, we will develop novel methodologies for the recover of 5' full length cDNA and differential display of 5 termini of mRNAs. ..
  40. QUANTITATION OF HYPOXIC TUMOR CELLS
    Peggy Olive; Fiscal Year: 2001
    ..To validate the models, expression of GFP will be analyzed in relation to proliferation, hypoxia marker binding, and clonogenicity following irradiation. ..
  41. PATHOGENESIS OF HUMAN GRANULOCYTIC EHRLICHIOSIS
    Ulrike Munderloh; Fiscal Year: 2001
    ....
  42. PHARMACOGENOMIC STUDY OF ANTICONVULSANT THERAPY
    THOMAS FERRARO; Fiscal Year: 2003
    ....
  43. KU COMPLEX AND TELOMERE END PROTECTION
    ALISON BERTUCH; Fiscal Year: 2003
    ..Dr. Lundblad's laboratory in the Department of Molecular and Human Genetics at the Baylor College of Medicine provides an excellent environment in which to develop my scientific skills. ..
  44. Oxidation of Apolipoprotein B-100 in LDL
    Chao Yuh Yang; Fiscal Year: 2004
    ....
  45. VITAMIN A STATUS/EFFECTS ON OLFACTORY NEURON MATURATION
    Mary Ann Asson Batres; Fiscal Year: 2004
    ..The PI is committed to a career in biomedical research with a focus on chemosensory systems. With this award, the PI can increase her independent research capability and become competitive for NIV R01-level funding. ..
  46. GENE-EXPRESSION CHANGE IN HUMAN BLOOD SAMPLES AND ANTI-DEPRESSANT RESPONSE
    SAMUEL SATHYANESAN; Fiscal Year: 2004
    ..Briefly, further studies would involve examening differences among different types of depressive disorders and the correlation between gene-expression changes and treatment-response. ..
  47. MOLECULAR TARGETS OF NITRIC OXIDE IN CARDIAC TRANSPLANTS
    GALEN PIEPER; Fiscal Year: 2004
    ..These studies will provide a novel mechanism to explain the pathogenesis of dyskinesis and graft failure during cardiac transplant rejection. ..
  48. DNA DAMAGE QUANTITATION BY SINGLE MOLECULE LASER SIZING
    John Sutherland; Fiscal Year: 2004
    ....
  49. Genes Controlling the Renal Handling of Phosphate
    Ralph Meyer; Fiscal Year: 2004
    ..Such intervention may improve the skeletal symptoms of patients with hypophosphatemic bone disease and improve their quality of life. ..
  50. Free radicals detection in aging brain by fullerenes
    VLADIMIR DIDENKO; Fiscal Year: 2003
    ..At a later stage free radicals will be monitored using magnetic resonance tracer and MRI detection. ..
  51. TRANSPOSON ACTIVE TRANSGENIC XIPHOPHORUS
    RONALD WALTER; Fiscal Year: 2003
    ..abstract_text> ..
  52. HIGH DENSITY LIPOPROTEIN SUBSPECIES AND CORONARY DISEASE
    Bela Asztalos; Fiscal Year: 2003
    ..The investigators state that these studies should provide better understanding about the diagnosis and treatment of HDL deficiency for the prevention of CHD. ..
  53. ENVIRONMENTAL LIGHT AND RETINAL MEMBRANE DEVELOPMENT
    Daniel Organisciak; Fiscal Year: 2003
    ..In addition, the time course of the appearance of cytoplasmic cytochrome C and the activation of cellular caspases will be determined by antibody-based techniques, immunocytochemistry and enzymatic analysis. ..
  54. MOB-5 IN COLORECTAL & PANCREATIC CANCER
    Peng Liang; Fiscal Year: 2001
    ..Studies in Specific Aim 2 are similar to those proposed for the rat gene with, in addition, an evaluation of a large series of tissue samples and an attempt to develop an Ab based clinical test using serum from cancer patients. ..
  55. TITINS ROLE IN MODULATING CARDIAC PASSIVE TENSION
    Marion Greaser; Fiscal Year: 2002
    ..Titin changes may be related to the hypertrophic response that commonly is brought about by high blood pressure and thus is a frequent problem in human health. ..
  56. Metabolism and Pharmacogenetics in MDMA-Induced Toxicity
    Rafael de la Torre; Fiscal Year: 2009
    ..A study will compare MDMA toxicity when co-adminsitered with the MRP1 inhibitor probenecid. ..
  57. Titin Splicing Mechanisms and Physical Implications
    Marion L Greaser; Fiscal Year: 2010
    ..In addition the rat mutation model we have developed should help to understand mechanisms of cardiac arrhythmia and sudden death. ..