Summary: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.

Top Publications

  1. ncbi Diagnostic screening for mucopolysaccharidoses by the dimethylmethylene blue method and two dimensional electrophoresis
    C K Chuang
    Division of Genetics and Metabolism, Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan
    Zhonghua Yi Xue Za Zhi (Taipei) 64:15-22. 2001
  2. pmc Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis
    J Walter
    Institute of Food Technology, University of Hohenheim, Stuttgart, Germany
    Appl Environ Microbiol 67:2578-85. 2001
  3. pmc Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed
    K Smalla
    Federal Biological Research Centre for Agriculture and Forestry, D 38104 Braunschweig, D 18051 Rostock, Germany
    Appl Environ Microbiol 67:4742-51. 2001
  4. pmc Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis
    Zhongtang Yu
    Department of Animal Sciences, The Ohio State University, 2027 Coffey Rd, Columbus, OH 43210, USA
    Appl Environ Microbiol 70:4800-6. 2004
  5. pmc Rapid identification of fungi by using the ITS2 genetic region and an automated fluorescent capillary electrophoresis system
    C Y Turenne
    Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Health Sciences Centre, Winnipeg, Manitoba R3A 1R9, Canada
    J Clin Microbiol 37:1846-51. 1999
  6. ncbi Efficient fractionation and improved protein identification by peptide OFFGEL electrophoresis
    Patric Hörth
    Agilent Technologies Research and Development and Marketing GmbH and Co KG, Hewlett Packard Strasse 8, 76337 Waldbronn, Germany
    Mol Cell Proteomics 5:1968-74. 2006
  7. pmc Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription-PCR in temperature gradient gel electrophoresis fingerprints
    A Felske
    Laboratory of Microbiology, Department of Biomolecular Sciences, Wageningen Agricultural University, 6703 CT Wageningen, The Netherlands
    Appl Environ Microbiol 64:4581-7. 1998
  8. pmc Isolation of bifidobacteria from breast milk and assessment of the bifidobacterial population by PCR-denaturing gradient gel electrophoresis and quantitative real-time PCR
    Rocio Martin
    Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands
    Appl Environ Microbiol 75:965-9. 2009
  9. ncbi Dielectrophoresis-based cell manipulation using electrodes on a reusable printed circuit board
    Kidong Park
    School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA
    Lab Chip 9:2224-9. 2009
  10. ncbi Electrically facilitated translocations of proteins through silicon nitride nanopores: conjoint and competitive action of diffusion, electrophoresis, and electroosmosis
    Matthias Firnkes
    Walter Schottky Institute, Technische Universitaet Munich, Am Coulombwall 3, 85748 Garching, Germany
    Nano Lett 10:2162-7. 2010

Detail Information

Publications357 found, 100 shown here

  1. ncbi Diagnostic screening for mucopolysaccharidoses by the dimethylmethylene blue method and two dimensional electrophoresis
    C K Chuang
    Division of Genetics and Metabolism, Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan
    Zhonghua Yi Xue Za Zhi (Taipei) 64:15-22. 2001
    ..a mucopolysaccharidosis (MPS) can be achieved by non-enzymatic screening methods, including two-dimensional electrophoresis (2-D EP), and the dimethylmethylene blue (DMB) method...
  2. pmc Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis
    J Walter
    Institute of Food Technology, University of Hohenheim, Stuttgart, Germany
    Appl Environ Microbiol 67:2578-85. 2001
    Denaturing gradient gel electrophoresis (DGGE) of DNA fragments generated by PCR with 16S ribosomal DNA-targeted group-specific primers was used to detect lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Leuconostoc, ..
  3. pmc Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed
    K Smalla
    Federal Biological Research Centre for Agriculture and Forestry, D 38104 Braunschweig, D 18051 Rostock, Germany
    Appl Environ Microbiol 67:4742-51. 2001
    ..fragments amplified by PCR from soil or rhizosphere bacterium DNA were analyzed by denaturing gradient gel electrophoresis (DGGE)...
  4. pmc Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis
    Zhongtang Yu
    Department of Animal Sciences, The Ohio State University, 2027 Coffey Rd, Columbus, OH 43210, USA
    Appl Environ Microbiol 70:4800-6. 2004
    Denaturing gradient gel electrophoresis (DGGE) has become a widely used tool to examine microbial diversity and community structure, but no systematic comparison has been made of the DGGE profiles obtained when different hypervariable (V)..
  5. pmc Rapid identification of fungi by using the ITS2 genetic region and an automated fluorescent capillary electrophoresis system
    C Y Turenne
    Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Health Sciences Centre, Winnipeg, Manitoba R3A 1R9, Canada
    J Clin Microbiol 37:1846-51. 1999
    ..We have found that most clinically significant fungal isolates can be differentiated by this method, and it therefore serves to be a promising tool for the rapid (<7 h) diagnosis of fungemia and other invasive fungal infections...
  6. ncbi Efficient fractionation and improved protein identification by peptide OFFGEL electrophoresis
    Patric Hörth
    Agilent Technologies Research and Development and Marketing GmbH and Co KG, Hewlett Packard Strasse 8, 76337 Waldbronn, Germany
    Mol Cell Proteomics 5:1968-74. 2006
    ..This paper illustrates the effectiveness of OFFGEL electrophoresis with the Agilent 3100 OFFGEL Fractionator for the fractionation of peptides...
  7. pmc Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription-PCR in temperature gradient gel electrophoresis fingerprints
    A Felske
    Laboratory of Microbiology, Department of Biomolecular Sciences, Wageningen Agricultural University, 6703 CT Wageningen, The Netherlands
    Appl Environ Microbiol 64:4581-7. 1998
    ..reverse transcription (RT)-PCR with bacterium-specific primers and were separated by temperature gradient gel electrophoresis (TGGE)...
  8. pmc Isolation of bifidobacteria from breast milk and assessment of the bifidobacterial population by PCR-denaturing gradient gel electrophoresis and quantitative real-time PCR
    Rocio Martin
    Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands
    Appl Environ Microbiol 75:965-9. 2009
    ..Bifidobacterial communities in breast milk were assessed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE), and their levels were estimated by quantitative real-time PCR (qRTi-PCR)...
  9. ncbi Dielectrophoresis-based cell manipulation using electrodes on a reusable printed circuit board
    Kidong Park
    School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA
    Lab Chip 9:2224-9. 2009
  10. ncbi Electrically facilitated translocations of proteins through silicon nitride nanopores: conjoint and competitive action of diffusion, electrophoresis, and electroosmosis
    Matthias Firnkes
    Walter Schottky Institute, Technische Universitaet Munich, Am Coulombwall 3, 85748 Garching, Germany
    Nano Lett 10:2162-7. 2010
    ..When electrophoresis and electroosmosis cancel each other out, diffusion becomes an effective (and bias-independent) mechanism ..
  11. pmc Electrophoretic deposition of biomaterials
    A R Boccaccini
    Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen Nuremberg, Erlangen, Germany
    J R Soc Interface 7:S581-613. 2010
  12. ncbi Continuous separation of colloidal particles using dielectrophoresis
    Nurul Amziah Md Yunus
    Department of Electrical and Electronic Engineering, Faculty of Engineering, Universiti Putra Malaysia, Selangor, Malaysia
    Electrophoresis 34:969-78. 2013
    ..The variation in device operation with applied signal voltage is presented and discussed in terms of separation efficiency, demonstrating 99.9% separation of a mixture of colloidal latex spheres...
  13. ncbi Polysaccharide analysis using carbohydrate gel electrophoresis: a method to study plant cell wall polysaccharides and polysaccharide hydrolases
    Florence Goubet
    Department of Biochemistry, Cambridge University, Building O, Downing Site, Cambridge, CB2 1QW, United Kingdom
    Anal Biochem 300:53-68. 2002
    ..Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of reducing ends of sugars and oligosaccharides with a fluorophore, followed ..
  14. ncbi Real-time cell electrophysiology using a multi-channel dielectrophoretic-dot microelectrode array
    Henry O Fatoyinbo
    Centre for Biomedical Engineering, School of Engineering, University of Surrey, Guildford, UK
    Electrophoresis 32:2541-9. 2011
    ..This technology shows the great potential for increasing our understanding of the role of electrophysiology in drug action, by observing the changes in electrical characteristics as they occur...
  15. ncbi Automated immunoassay system for AFP-L3% using on-chip electrokinetic reaction and separation by affinity electrophoresis
    Chiaki Kagebayashi
    Osaka Research Laboratory, New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries Ltd, 6 1 Takada cho, Amagasaki, Hyogo Prefecture, Japan
    Anal Biochem 388:306-11. 2009
    ..causing separation of the fucosylated isoform, AFP-L3, from the nonfucosylated AFP-L1 by lectin affinity electrophoresis. Laser-induced-fluorescence (LIF) detection was used to quantitate the labeled immunocomplexes...
  16. pmc Electrodeless dielectrophoresis of single- and double-stranded DNA
    Chia Fu Chou
    Department of Physics, Princeton University, New Jersey 08544, USA
    Biophys J 83:2170-9. 2002
    ..A possible mechanism for DNA polarization in ionic solution is discussed based on the frequency, viscosity, and field dependence of the observed trapping force...
  17. ncbi Identification and typing of Malassezia yeasts using amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD) and denaturing gradient gel electrophoresis (DGGE)
    B Theelen
    Centraalbureau voor Schimmelcultures, Yeast Division, Utrecht, The Netherlands
    FEMS Yeast Res 1:79-86. 2001
    Three molecular tools, amplified fragment length polymorphism (AFLP), denaturing gradient gel electrophoresis (DGGE) and random amplified polymorphic DNA (RAPD) analysis, were explored for their usefulness to identify isolates of ..
  18. pmc Native gel electrophoresis of human telomerase distinguishes active complexes with or without dyskerin
    Laura Gardano
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 40:e36. 2012
    ..We used oligonucleotide-based affinity purification of hTR followed by native gel electrophoresis and in-gel telomerase activity detection to query the composition of telomerase at different purification ..
  19. ncbi Dielectrophoretic concentration and separation of live and dead bacteria in an array of insulators
    Blanca H Lapizco-Encinas
    Microfluidics Department, Sandia National Laboratories, PO Box 969, MS 9951, Livermore, California 94551, USA
    Anal Chem 76:1571-9. 2004
    ..2-microm carboxylate-modified polystyrene particles. This first application of iDEP for simultaneous live/dead bacteria separation and concentration illustrates its potential as a front-end method for bacterial analysis...
  20. ncbi Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns
    N Fromin
    Microbiology Laboratory, University of Neuchatel, Switzerland
    Environ Microbiol 4:634-43. 2002
    ..Among these techniques, fingerprinting methods such as denaturing gel electrophoresis (DGE, including the three options: DGGE, TGGE and TTGE) has been applied to environmental samples over this ..
  21. pmc Analysis of fungal diversity in the wheat rhizosphere by sequencing of cloned PCR-amplified genes encoding 18S rRNA and temperature gradient gel electrophoresis
    E Smit
    Microbiological Laboratory for Health Protection, National Institute of Public Health and the Environment RIVM, NL 3720 BA Bilthoven, The Netherlands
    Appl Environ Microbiol 65:2614-21. 1999
    ..Similarity values ranged from 0.676 to 1. Temperature gradient gel electrophoresis (TGGE) analysis of the fungal community in the wheat rhizosphere of a microcosm experiment was carried out ..
  22. pmc Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA as well as DNA fragments coding for 16S rRNA
    B M Duineveld
    Institute of Evolutionary and Ecological Sciences, Leiden University 2300 RA Leiden, The Netherlands
    Appl Environ Microbiol 67:172-8. 2001
    ..ribosomal DNA (rDNA) and 16S rRNA, respectively, and the products were subjected to denaturing gradient gel electrophoresis (DGGE)...
  23. pmc Dielectrophoretic manipulation of cells with spiral electrodes
    X B Wang
    Department of Experimental Pathology, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Biophys J 72:1887-99. 1997
    ..Applications of spiral electrode arrays, including the isolation of cells of clinical relevance, are discussed...
  24. ncbi Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
    K M Ririe
    Idaho Technology Inc, Idaho Falls 83402, USA
    Anal Biochem 245:154-60. 1997
    ..Desired products can be distinguished from undesirable products, in many cases eliminating the need for gel electrophoresis. Analysis of melting curves can extend the dynamic range of initial template quantification when ..
  25. ncbi Proteomic analysis of human vitreous fluid by fluorescence-based difference gel electrophoresis (DIGE): a new strategy for identifying potential candidates in the pathogenesis of proliferative diabetic retinopathy
    M Garcia-Ramirez
    Diabetes Research Unit, Endocrinology Division, Vall d Hebron University Hospital Research Institute, Passeig Vall d Hebron 119 129, 08035 Barcelona, Spain
    Diabetologia 50:1294-303. 2007
    ..The mRNA of proteins differentially produced was also assessed in the retinas from diabetic and non-diabetic donors...
  26. ncbi Electrical forces for microscale cell manipulation
    Joel Voldman
    Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Annu Rev Biomed Eng 8:425-54. 2006
    Electrical forces for manipulating cells at the microscale include electrophoresis and dielectrophoresis...
  27. ncbi Electronic manipulation of DNA, proteins, and nanoparticles for potential circuit assembly
    Lifeng Zheng
    Electrical Engineering and Computer Science, University of California, Irvine, CA 92697 2625, USA
    Biosens Bioelectron 20:606-19. 2004
    ..The technique of simultaneously measuring resistance while using dielectrophoresis to trap nanoscale objects should find broad applicability...
  28. ncbi Measuring the dielectric properties of herpes simplex virus type 1 virions with dielectrophoresis
    Michael P Hughes
    Department of Electronics and Electrical Engineering, Bioelectronic Research Centre, University of Glasgow, Scotland, Glasgow, UK
    Biochim Biophys Acta 1571:1-8. 2002
    ..It is shown that the observed changes in the dielectrophoretic spectrum, and the variations in the dielectric properties of the virus concur with the expected physiological effects of these agents...
  29. ncbi Peptide enrichment and protein fractionation using selective electrophoresis
    Linda Ly
    Bioanalytical Mass Spectrometry Facility, The University of New South Wales, Sydney, Australia
    Proteomics 8:4197-208. 2008
    ..The utility of this approach is demonstrated with the identification (with at least 2 ppm mass accuracy) of 76 unique peptides, equating to 22 proteins enriched to the 1-5 kDa fraction of the MF10...
  30. ncbi Nonlinear electrophoresis for purification of soil DNA for metagenomics
    Katja Engel
    Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON, Canada N2L 3G1
    J Microbiol Methods 88:35-40. 2012
    ..Using three different composite soil samples, we compared a novel DNA purification technique using nonlinear electrophoresis on the synchronous coefficient of drag alteration (SCODA) instrument with alternate purification methods such ..
  31. ncbi Single-molecule electrophoresis of beta-hairpin peptides by electrical recordings and Langevin dynamics simulations
    Carl P Goodrich
    J Phys Chem B 111:3332-5. 2007
    ..This study demonstrates that the interaction between a polypeptide and a beta-barrel protein pore is dependent on the folding features of the polypeptide...
  32. pmc Detritus-dependent development of the microbial community in an experimental system: qualitative analysis by denaturing gradient gel electrophoresis
    E J van Hannen
    Department of Microbial Ecology, Centre for Limnology, Netherlands Institute of Ecology, 3600 BG Maarssen, The Netherlands
    Appl Environ Microbiol 65:2478-84. 1999
    ..Denaturing gradient gel electrophoresis (DGGE) of the 16S and 18S rRNA genes was used to monitor the development of the bacterial community structure ..
  33. ncbi Analysis of IgA1 O-glycans in IgA nephropathy by fluorophore-assisted carbohydrate electrophoresis
    A C Allen
    Department of Nephrology, Leicester General Hospital, United Kingdom
    J Am Soc Nephrol 10:1763-71. 1999
    ..This is the first study using fluorophore-assisted carbohydrate electrophoresis (FACE) to analyze IgA1 O-glycans in IgAN and controls...
  34. pmc Microsample preparation by dielectrophoresis: isolation of malaria
    Peter Gascoyne
    Department of Molecular Pathology, University of Texas, M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA
    Lab Chip 2:70-5. 2002
    ..By simple adjustment of the electrical field parameters, other cell subpopulations that characterise disease, such as residual cancer cells in blood, can be similarly isolated and analysed...
  35. ncbi The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis
    F H Liu
    Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    J Appl Microbiol 106:952-66. 2009
    ..To identify the bacterial and archaeal composition in a mesophilic biogas digester treating pig manure and to compare the consistency of two 16S rDNA-based methods to investigate the microbial structure...
  36. pmc Preimplantation mouse embryo selection guided by light-induced dielectrophoresis
    Justin K Valley
    Berkeley Sensor and Actuator Center, Electrical Engineering and Computer Sciences, University of California, Berkeley, California, United States of America
    PLoS ONE 5:e10160. 2010
    ..Light-induced dielectrophoresis provides a non-invasive, quantitative, and reproducible means to select embryos for applications including IVF transfer and embryonic stem cell harvest...
  37. ncbi Cellular dielectrophoresis: applications to the characterization, manipulation, separation and patterning of cells
    Zachary R Gagnon
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA
    Electrophoresis 32:2466-87. 2011
    ..However, there is need for a critical report to integrate the many new features of DEP for cellular applications. Here, a review of the basic theory and current applications of DEP, specifically for cells, is presented...
  38. pmc Rapid identification of gram-negative bacteria with and without CTX-M extended-spectrum β-lactamase from positive blood culture bottles by PCR followed by microchip gel electrophoresis
    Shin Ichi Fujita
    Department of Laboratory Sciences, School of Health Sciences, Kanazawa University, 5 11 80 Kodatuno, Kanazawa 920 0942, Japan
    J Clin Microbiol 49:1483-8. 2011
    ..transcribed spacer (ITS) and the CTX-M extended-spectrum β-lactamase (ESBL) followed by microchip gel electrophoresis (MGE) for direct identification and CTX-M detection of Gram-negative bacteria (GNB) from positive blood ..
  39. ncbi Cancer, pre-cancer and normal oral cells distinguished by dielectrophoresis
    H J Mulhall
    Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK
    Anal Bioanal Chem 401:2455-63. 2011
    ..To use this technique as an OSCC detection tool in a clinical setting, further characterisation and refinement is warranted...
  40. ncbi DNA manipulation by means of insulator-based dielectrophoresis employing direct current electric fields
    Roberto C Gallo-Villanueva
    Departamento de Biotecnología e Ingeniería de Alimentos y Centro de Biotecnología, Tecnológico de Monterrey, Monterrey, Nuevo Leon, Mexico
    Electrophoresis 30:4195-205. 2009
    ..The results presented here demonstrate the potential of insulator-based DEP for DNA concentration, and open the possibility for fast DNA manipulation for laboratory and large-scale applications...
  41. pmc A novel universal primer-multiplex-PCR method with sequencing gel electrophoresis analysis
    Wentao Xu
    Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
    PLoS ONE 7:e22900. 2012
    ..PCR products were analyzed on sequencing gel electrophoresis (SGE) which had the advantage of exhibiting extraordinary resolution...
  42. ncbi Screening of antibiotic susceptibility to β-lactam-induced elongation of Gram-negative bacteria based on dielectrophoresis
    Cheng Che Chung
    Institute of Nanotechnology and Microsystems Engineering, National Cheng Kung University, Tainan, Taiwan
    Anal Chem 84:3347-54. 2012
  43. ncbi Shotgun proteomics: a relative quantitative approach using Off-Gel electrophoresis and LC-MS/MS
    Laurent Geiser
    Swiss Centre for Applied Human Toxicology, Geneva University, Geneva, Switzerland
    Methods Mol Biol 681:459-72. 2011
    ..two orthogonal techniques: first, peptides are separated according to their isoelectric point (pI) by Off-Gel electrophoresis (OGE), a relatively new isoelectric focusing (IEF) technique; after peptide purification, they are then ..
  44. pmc Protein dielectrophoresis: advances, challenges, and applications
    Asuka Nakano
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA
    Electrophoresis 34:1085-96. 2013
    ..We further point out the variations in experimental conditions used in the past to study the somewhat 20 proteins as well as the implications of protein molecular structure to the DEP response...
  45. ncbi Silicon insulator-based dielectrophoresis devices for minimized heating effects
    Phillip Zellner
    VT MEMS Lab, Virginia Tech, Blacksburg, VA 24061, USA
    Electrophoresis 33:2498-507. 2012
    ..Trapping and separation of 1 and 2 μm polystyrene particles was demonstrated. These results demonstrate the feasibility of high-performance silicon iDEP devices for the next generation of sorting and concentration microsystems...
  46. ncbi Dielectrophoretic manipulation and separation of microparticles using curved microelectrodes
    Khashayar Khoshmanesh
    Centre for Intelligent Systems Research, Deakin University, Victoria, Australia
    Electrophoresis 30:3707-17. 2009
    ..Experiments demonstrate the capability of the system as a field flow fraction tool for sorting microparticles according to their dimensions and dielectric properties...
  47. ncbi Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation
    Michael A Quail
    Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK
    Electrophoresis 33:3521-8. 2012
    ..Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes...
  48. ncbi Dielectrophoresis of lambda-DNA using 3D carbon electrodes
    Rodrigo Martínez-Duarte
    Laboratory of Microsystems, Ecole Polytechnique Federale de Lausanne, Switzerland
    Electrophoresis 34:1113-22. 2013
  49. pmc Assessment of multidrug resistance reversal using dielectrophoresis and flow cytometry
    Fatima H Labeed
    Centre of Biomedical Engineering, School of Engineering, and Division of Oncology, Postgraduate Medical School, University of Surrey, Guildford, Surrey, United Kingdom
    Biophys J 85:2028-34. 2003
  50. ncbi Characterization and identification of proteases secreted by Aspergillus fumigatus using free flow electrophoresis and MS
    Madlen Neustadt
    Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany
    Electrophoresis 30:2142-50. 2009
    ..of secreted proteases, the culture supernatant of Aspergillus fumigatus was fractionated using free flow electrophoresis (Becton Dickinson)...
  51. pmc Selection of mammalian cells based on their cell-cycle phase using dielectrophoresis
    Unyoung Kim
    Departments of Mechanical Engineering, University of California, Santa Barbara, CA 93106, USA
    Proc Natl Acad Sci U S A 104:20708-12. 2007
    ..This work illustrates the feasibility of using laminar flow and electrokinetic forces for the efficient, noninvasive separation of living cells...
  52. ncbi Species assignation of Leishmania from human and canine American tegumentary leishmaniasis cases by multilocus enzyme electrophoresis in North Argentina
    Jorge D Marco
    Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan
    Am J Trop Med Hyg 72:606-11. 2005
    ..plus one from a dog with CL in Salta and Corrientes Provinces, Argentina, were studied by multilocus enzyme electrophoresis. Thirteen of the stocks from humans were grouped in two zymodemes; nine termed as KMS 1, four as KMS 2, and ..
  53. ncbi Comparison of two commercial molecular assays for simultaneous detection of respiratory viruses in clinical samples using two automatic electrophoresis detection systems
    L E S Bruijnesteijn van Coppenraet
    Laboratory of Medical Microbiology and Infectious Diseases, Isala Clinics, Stilobadstraat 3, 8021 AB Zwolle, The Netherlands
    J Virol Methods 169:188-92. 2010
    ..In addition, the positive detections of MLPA and DPO were identified using two different automatic electrophoresis systems...
  54. ncbi Large pore gels to separate mega protein complexes larger than 10 MDa by blue native electrophoresis: isolation of putative respiratory strings or patches
    Valentina Strecker
    Molecular Bioenergetics, Medical School, Goethe University Frankfurt, Frankfurt am Main, Germany
    Proteomics 10:3379-87. 2010
    Here, we expand the application of blue native electrophoresis to the separation of mega protein complexes larger than 10 MDa by introducing novel large pore acrylamide gels...
  55. pmc Differentiation of Mycoplasma species by 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis fingerprinting
    Laura McAuliffe
    Mycoplasma Group, Department of Bacterial Diseases, Veterinary Laboratories Agency Weybridge, Surrey, KT15 3NB, United Kingdom
    J Clin Microbiol 41:4844-7. 2003
    Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species of veterinary significance. Twenty-seven (85%) species could be differentiated by DGGE...
  56. ncbi Hemoglobin electrophoresis and hemoglobinopathies in Kuwait
    R Marouf
    Department of Pathology, Faculty of Medicine, Kuwait University, PO Box 24923, 13110 Safat, Kuwait
    Med Princ Pract 11:38-41. 2002
    To analyze the results of hemoglobin electrophoresis (HE) in the routine laboratory of a tertiary hospital in Kuwait and to review the common types of hemoglobinopathies prevalent in the country.
  57. pmc Isolation of DNA fragments associated with methylated CpG islands in human adenocarcinomas of the lung using a methylated DNA binding column and denaturing gradient gel electrophoresis
    M Shiraishi
    Oncogene Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo ku 104 0045, Tokyo, Japan
    Proc Natl Acad Sci U S A 96:2913-8. 1999
    ..were isolated on the basis of their reduced rate of strand dissociation during denaturing gradient gel electrophoresis. Approximately 1,000 clones, corresponding to 0...
  58. ncbi Soil microbial community response to land use change in an agricultural landscape of western Kenya
    D A Bossio
    World Agroforestry Center, ICRAF, P O Box 30677, Nairobi, Kenya
    Microb Ecol 49:50-62. 2005
    ..For all sites, principal component analysis of 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) profiles revealed that soil type was the key determinant of total bacterial community structure, with ..
  59. ncbi Analysis of pharmaceutical heparins and potential contaminants using (1)H-NMR and PAGE
    Zhenqing Zhang
    Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
    J Pharm Sci 98:4017-26. 2009
    ..to deaminative cleavage, a method widely used to depolymerize heparin, was evaluated with polyacrylamide gel electrophoresis to detect impurities and contaminants, giving limits of detection (LOD) ranging from 0.1% to 5%...
  60. ncbi Electrophoresis techniques to investigate defects in oxidative phosphorylation
    Maria Antonietta Calvaruso
    Nijmegen Center for Mitochondrial Disorders at the Department of Pediatrics, Laboratory of Pediatrics and Neurology, Radboud University Nijmegen Medical Centre, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Methods 46:281-7. 2008
    ..Blue native electrophoresis has become a crucial tool to investigate OXPHOS-related defects in mitochondrial disease patients...
  61. ncbi Dielectrophoresis-activated multiwell plate for label-free high-throughput drug assessment
    Kai F Hoettges
    School of Engineering, and School of Biomedical and Molecular Science, University of Surrey, Guildford, Surrey GU2 7XH, UK
    Anal Chem 80:2063-8. 2008
  62. ncbi Single-cell trapping utilizing negative dielectrophoretic quadrupole and microwell electrodes
    Ling Sheng Jang
    Department of Electrical Engineering and Center for Micro Nano Science and Technology, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan
    Biosens Bioelectron 24:3637-44. 2009
    ..Manipulation of particle motion using ACET-induced fluid flow to the target trap is modeled numerically and is in good agreement with the experimental results...
  63. ncbi Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresis
    Ningguo Gao
    Department of Pharmacology, UT Southwestern Medical Center, Dallas, TX, USA
    Methods Enzymol 415:3-20. 2006
    ..Here we describe the application of a facile non-radioactive technique, fluorophore-assisted carbohydrate electrophoresis (FACE), which circumvents these limitations...
  64. ncbi Free-flow electrophoresis for purification of plant mitochondria by surface charge
    Holger Eubel
    ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, M316, Crawley, WA 6009, Australia
    Plant J 52:583-94. 2007
    ..The suitability of free-flow electrophoresis to assist conventional, centrifugation-based techniques in the preparation of plant mitochondria from green ..
  65. pmc Rapid assessment of early biophysical changes in K562 cells during apoptosis determined using dielectrophoresis
    Sue Chin
    Centre for Biomedical Engineering, School of Engineering, University of Surrey, Guildford, Surrey, UK
    Int J Nanomedicine 1:333-7. 2006
    ..This method therefore allows a far more rapid detection method than existing biochemical marker methods...
  66. ncbi Differences in the biophysical properties of membrane and cytoplasm of apoptotic cells revealed using dielectrophoresis
    Fatima H Labeed
    Centre for Biomedical Engineering, School of Engineering, University of Surrey, Guildford, Surrey GU2 7XH, UK
    Biochim Biophys Acta 1760:922-9. 2006
    ..The use of dielectrophoresis for monitoring apoptosis offers a number of benefits as it is both rapid and non-invasive. It can also be used in parallel with other assays in high-throughput screening applications...
  67. pmc Detection and identification of Lactobacillus species in crops of broilers of different ages by using PCR-denaturing gradient gel electrophoresis and amplified ribosomal DNA restriction analysis
    Le Luo Guan
    Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5
    Appl Environ Microbiol 69:6750-7. 2003
    ..throughout the broiler production period (0 to 42 days) using PCR combined with denaturing gradient gel electrophoresis (PCR-DGGE) and selective bacteriological culture of lactobacilli followed by amplified ribosomal DNA ..
  68. ncbi Proteomic analysis of the secretome of human umbilical vein endothelial cells using a combination of free-flow electrophoresis and nanoflow LC-MS/MS
    David Guillen Tunica
    Pharmacology Laboratory CDB, Hospital Clinic, IDIBAPS, Barcelona University, CIBERehd, Barcelona, Spain
    Proteomics 9:4991-6. 2009
    ..study, we accomplished to map the secretome of human umbilical vein endothelial cells by combining free-flow electrophoresis with nanoflow LC-MS/MS...
  69. ncbi Gel-eluted liquid fraction entrapment electrophoresis: an electrophoretic method for broad molecular weight range proteome separation
    John C Tran
    Department of Chemistry, Dalhousie University, 6274 Coburg Road, Halifax, Nova Scotia, Canada B3H 4J3
    Anal Chem 80:1568-73. 2008
    ..well-established as a technique for protein purification, the application of continuous elution tube gel electrophoresis to proteome fractionation remains problematic...
  70. ncbi Extraction and purification of DNA in rhizosphere soil samples for PCR-DGGE analysis of bacterial consortia
    R Maarit Niemi
    Finnish Environment Institute, PO Box 140 FIN 00251, Helsinki, Finland
    J Microbiol Methods 45:155-65. 2001
    ..in PCR using universal bacterial primers and the PCR products were analysed with denaturing gradient gel electrophoresis (DGGE) for the visualisation of DNA bands representing dominant bacterial species...
  71. ncbi Non-denaturing polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinemia
    Dirk J Blom
    Lipidology Division of the Department of Medicine and MRC Cape Heart Group, University of Cape Town, Faculty of Health Sciences, Observatory, South Africa
    J Lipid Res 44:212-7. 2003
    ..The demonstration of a broad beta-band on agarose gel electrophoresis of plasma is an insensitive method and ultracentrifugation is an impractical method of diagnosing this ..
  72. ncbi Three-layer sandwich gel electrophoresis: a method of salt removal and protein concentration in proteome analysis
    Ting Liu
    Department of Chemistry, University of Kentucky, Lexington, Kentucky 40506 0055, USA
    J Proteome Res 7:4256-65. 2008
    ..Our data suggest that the Three-layer Sandwich Gel Electrophoresis (TSGE) protocol can solve this problem and provide near quantitative recovery of extremely low concentration ..
  73. ncbi Automated dielectrophoretic characterization of Mycobacterium smegmatis
    Benjamin G Hawkins
    Department of Biomedical Engineering, Cornell University, Ithaca, New York, United States
    Anal Chem 83:3507-15. 2011
    ..This work represents the first such characterization of Mycobacteria and highlights the potential for DEP measurements to measure changes in mycobacterial membrane properties associated with chemical treatments or genetic mutation...
  74. ncbi Evaluation of denaturing gradient gel electrophoresis to differentiate Escherichia coli populations in secondary environments
    Von Sigler
    Department of Earth, Ecological and Environmental Sciences, University of Toledo, Toledo, OH 43606, USA
    Environ Microbiol 8:1703-11. 2006
    ..The objective of this study was to determine the ability of denaturing gradient gel electrophoresis (DGGE) to differentiate mixed assemblages of E. coli in the natural environment...
  75. ncbi Parallel measurements of drug actions on Erythrocytes by dielectrophoresis, using a three-dimensional electrode design
    Y Hübner
    School of Engineering, University of Surrey, Guildford, Surrey, GU2 7XH, UK
    IEE Proc Nanobiotechnol 152:150-4. 2005
    ..The technique is directly compatible with the industry-standard 1536 well-plate analysis technique...
  76. ncbi Dielectric characterization of bacterial cells using dielectrophoresis
    A Sanchis
    Departamento de Fisica Aplicada III, Facultad de Ciencias Fisicas, Universidad Complutense de Madrid, Madrid, Spain
    Bioelectromagnetics 28:393-401. 2007
    ..We show the usefulness of this simple method to extract significant information about the electrical properties of Gram-negative and -positive bacteria...
  77. ncbi Phylogenetic diversity of bat trypanosomes of subgenus Schizotrypanum based on multilocus enzyme electrophoresis, random amplified polymorphic DNA, and cytochrome b nucleotide sequence analyses
    C Barnabe
    Génétique des Maladies Infectieuses, Unité Mixte de Recherche Centre National de la Recherche Scientifique, Institut de Recherche pour le Developpement, n 9926 IRD, BP 64501, 34394 Montpellier Cedex 05, France
    Infect Genet Evol 2:201-8. 2003
    ..from bats (Chiroptera) and belonging to the subgenus Schizotrypanum were analyzed by multilocus enzyme electrophoresis (MLEE) at 22 loci, random amplified polymorphic DNA (RAPD) with 14 primers and/or cytochrome b nucleotide ..
  78. ncbi Stacking in capillary zone electrophoresis
    Z K Shihabi
    Pathology Department, Wake Forest University, Baptist Medical Center, Winston Salem, NC 27157, USA
    J Chromatogr A 902:107-17. 2000
    ..can concentrate the sample (stack) easily on the capillary before the separation step of capillary zone electrophoresis (CZE)...
  79. ncbi Enhanced separation of membranes during free flow zonal electrophoresis in plants
    Bronwyn J Barkla
    Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, A P 510 3, Colonia Miraval, Cuernavaca, Morelos, Mexico
    Anal Chem 79:5181-7. 2007
    Free flow zonal electrophoresis (FFZE) is a versatile technique that allows for the separation of cells, organelles, membranes, and proteins based on net surface charge during laminar flow through a thin aqueous layer...
  80. ncbi Derivatization of carbohydrates for analysis by chromatography; electrophoresis and mass spectrometry
    David J Harvey
    Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, UK
    J Chromatogr B Analyt Technol Biomed Life Sci 879:1196-225. 2011
    ..All of these groups have been the targets of derivatization and this review attempts to summarise the main methods used for these various functional groups...
  81. ncbi In-column field-amplified sample stacking of biogenic amines on microfabricated electrophoresis devices
    Nigel P Beard
    Department of Chemistry, Imperial College of Science, Technology and Medicine, South Kensington, London, UK
    Electrophoresis 24:732-9. 2003
    ..This design feature radically reduces the complexity in chip structures and associated chip operation. The approach is applied to the analysis of fluorescently labelled biogenic amines affording detection at concentrations down to 20 pM...
  82. ncbi Multilocus enzyme electrophoresis: a valuable technique for providing answers to problems in parasite systematics
    R H Andrews
    Department of Microbiology and Immunology, The University of Adelaide, SA, Australia
    Int J Parasitol 29:213-53. 1999
    The aim of this review is to highlight the effectiveness of the technique of multilocus enzyme electrophoresis in answering questions relating to the systematics of parasites and to highlight errors in the way the technique has been used ..
  83. ncbi Noncompetitive immunoassay for carcinoembryonic antigen in human serum by microchip electrophoresis for cancer diagnosis
    Fanggui Ye
    Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Education, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 51004, PR China
    Clin Chim Acta 411:1058-62. 2010
    ..We describe a microchip electrophoresis (MCE)-based noncompetitive immunoassay technique for assaying CEA in human serum for cancer diagnosis.
  84. ncbi The method of single-nucleotide variations detection using capillary electrophoresis and molecular beacons
    Jinhui Wang
    Department of Clinical Laboratory, The Second Affiliated Hospital of Harbin Medical University, Harbin, China
    Mol Biol Rep 36:1903-8. 2009
    We demonstrate that single-nucleotide variations in a DNA sequence can be detected using capillary electrophoresis (CE) and molecular beacons (MBs)...
  85. ncbi Electrophoresis of cells and the biological relevance of surface charge
    Jitendra N Mehrishi
    University of Cambridge, Cambridge, United Kingdom
    Electrophoresis 23:1984-94. 2002
    Recent developments in electrophoresis of cells are reviewed...
  86. ncbi Microbicides for HIV/AIDS. 2. Electrophoretic fingerprinting of CD4+ T-cell model systems
    D Fairhurst
    International Partnership for Microbicides, Silver Spring, MD 20910, USA
    Langmuir 23:2680-7. 2007
  87. pmc Sampling techniques for single-cell electrophoresis
    Christine Cecala
    Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Analyst 137:2922-9. 2012
    ..The high sensitivity and low sample consumption of capillary and on-chip electrophoresis, when integrated with fluorescence, electrochemical, and mass spectrometric detection methods, offer an ideal ..
  88. pmc Improving matrix metalloproteinase-2 specific response of a hydrogel system using electrophoresis
    Yu Zhang
    Department of Biopharmaceutical Sciences, University of Illinois, Chicago, IL 60612 7231, USA
    Int J Pharm 429:31-7. 2012
    ..After electrophoresis, the nonspecific peptide release in the absence of MMP-2 was minimized...
  89. ncbi Comparison of three methods for single nucleotide polymorphism typing for DNA bank studies: sequence-specific oligonucleotide probe hybridisation, TaqMan liquid phase hybridisation, and microplate array diagonal gel electrophoresis (MADGE)
    J W Holloway
    Human Genetics Research Division, University of Southampton, Southampton General Hospital, Southampton, UK
    Hum Mutat 14:340-7. 1999
    ..Throughputs achievable are similar, but there are major differences in cost and time for set-up, equipment, consumables, and staff time, which may determine the choice for individual laboratories...
  90. ncbi Population structure and genetic typing of Trypanosoma cruzi, the agent of Chagas disease: a multilocus enzyme electrophoresis approach
    C Barnabe
    Centre d Etudes sur le Polymorphisme des Microorganismes, Unité Mixte de Recherche Centre National de la Recherche Scientifique Institut de Recherche pour le Développement 9926, Montpellier, France
    Parasitology 120:513-26. 2000
    A set of 434 Trypanosoma cruzi stocks from a wide ecogeographical range was analysed by Multilocus Enzyme Electrophoresis for 22 genetic loci...
  91. ncbi Comparison of slab gel electrophoresis and capillary electrophoresis for the detection of the fluorescently labeled polymerase chain reaction products of short tandem repeat fragments
    D L Deforce
    Laboratory for Pharmaceutical Biotechnology, University of Ghent, Belgium
    J Chromatogr A 806:149-55. 1998
    The sizing capability of slab gel electrophoresis for short tandem repeat (STR) fragments was compared to the sizing capability of capillary electrophoresis (CE)...
  92. ncbi Comparison between gradient gel electrophoresis and nuclear magnetic resonance spectroscopy in estimating coronary heart disease risk associated with LDL and HDL particle size
    Benoit J Arsenault
    Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands
    Clin Chem 56:789-98. 2010
    Gradient gel electrophoresis (GGE) and nuclear magnetic resonance (NMR) spectroscopy are both widely accepted methods for measuring LDL and HDL particle size...
  93. ncbi Analysis of Haemophilus parasuis by multilocus enzyme electrophoresis
    P J Blackall
    Animal Research Institute, Yeerongpilly, OLD, Australia
    Vet Microbiol 56:125-34. 1997
    ..Australian isolates and eight reference strains of Haemophilus parasuis was examined using multilocus enzyme electrophoresis. Thirty-four electrophoretic types (ETs) were recognized with a mean diversity per locus of 0.405...
  94. ncbi The distribution of the tail moments in single cell gel electrophoresis (comet assay) obeys a chi-square (chi2) not a gaussian distribution
    E Bauer
    Institute of Molecular Biotechnology, Jena, Germany
    Mutat Res 398:101-10. 1998
    The parameter tail moment in single cell gel electrophoresis (comet assay) is calculated as the product of the two values: the percentage of DNA in the comet tail and the tail length in microm...
  95. ncbi Microchip electrophoresis-based separation of DNA
    Lihua Zhang
    Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation JST, Shomachi, Tokushima 770 8505, Japan
    J Pharm Biomed Anal 30:1645-54. 2003
    ..This review is focused on the microchip electrophoresis-based separation of DNA...
  96. ncbi Methanotrophic diversity in high arctic wetlands on the islands of Svalbard (Norway)--denaturing gradient gel electrophoresis analysis of soil DNA and enrichment cultures
    Ingvild Wartiainen
    Department of Biology, Faculty of Science, University of Tromosø, Norway
    Can J Microbiol 49:602-12. 2003
    ..PCR with PCR of the highly variable V3 region of the 16S rRNA gene and then by denaturing gradient gel electrophoresis (DGGE). Selected bands were sequenced for identification...
  97. ncbi One-step separation of endocytic organelles, Golgi/trans-Golgi network and plasma membrane by density gradient electrophoresis
    R Lindner
    Department of Cell Biology, Center of Anatomy Hannover Medical School, Germany
    Electrophoresis 22:386-93. 2001
    ..Here a density gradient electrophoresis protocol is described that allows the fractionation of these organelles in one step...
  98. pmc Numerical simulation of gel electrophoresis of DNA knots in weak and strong electric fields
    C Weber
    Institut Romand de Recherche Numérique en Physique des Matériaux IRRMA, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
    Biophys J 90:3100-5. 2006
    Gel electrophoresis allows one to separate knotted DNA (nicked circular) of equal length according to the knot type. At low electric fields, complex knots, being more compact, drift faster than simpler knots...
  99. ncbi Analysis of protein composition of red wine in comparison with rosé and white wines by electrophoresis and high-pressure liquid chromatography-mass spectrometry (HPLC-MS)
    Petra Wigand
    Institute of Molecular Biophysics of the Johannes Gutenberg University Mainz, Germany
    J Agric Food Chem 57:4328-33. 2009
    ..The occurrence and distribution of LTP depend on the wine variety...
  100. ncbi Water-soluble complexes formed by natural polyphenols and bovine serum albumin: evidence from gel electrophoresis
    Miwako Kusuda
    Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan
    Biosci Biotechnol Biochem 70:152-60. 2006
    ..complexes from combinations of various polyphenols and bovine serum albumin (BSA) was analyzed by gel electrophoresis. Sodium dodecylsulfate (SDS) and native polyacrylamide gel electrophoretic (PAGE) analyses clearly showed ..
  101. ncbi Secretome of human bronchial epithelial cells in response to the fungal pathogen Aspergillus fumigatus analyzed by differential in-gel electrophoresis
    A Fekkar
    Institut National de la Sante et de la Recherche Medicale INSERM, U945, Universite Pierre et Marie Curie Paris 6, Paris, France
    J Infect Dis 205:1163-72. 2012
    ..More recently, the contribution of the airway epithelial barrier has been assessed and studies have shown that it was able to sense and react to the Aspergillus infection, for example, by producing cytokines...

Research Grants91

    Gary Cecchini; Fiscal Year: 2013
    ..and respirometry methods are used to assess catalytic activity, and Western blots and 1D- and 2D-gel electrophoresis will be used to assess protein-protein interactions...
    Pi Wan Cheng; Fiscal Year: 2010
    ..They will be characterized by electrophoresis mobility shift assay and chromatin immunoprecipitation assay...
  3. Characterization of epsilon-sarcoglycan interacting proteins in mouse brain
    Yuqing Li; Fiscal Year: 2012
    ..be performed using specific monoclonal antibody against ?-SG followed by separation of these proteins by gel electrophoresis. The bands only appeared in the WT mouse brain samples should be ?-SG and the interacting proteins and the ..
  4. Extracellular ubiquitin: role in myocyte apoptosis and myocardial remodeling
    Krishna Singh; Fiscal Year: 2010
    ..Using 2-dimensional gel electrophoresis followed by MALDI TOF and MS/MS, we identified ubiquitin in the conditioned media of ARVMs treated with ?-AR ..
  5. Pilot Studies of the Effect of Aging on Mucociliary Clearance
    Lawrence E Ostrowski; Fiscal Year: 2013
    ..The amount of secreted and total airway mucin in the lung will be quantified by agarose gel electrophoresis and Western blotting...
  6. Controlled delivery of polypeptide hormone calcitonin
    Jagdish Singh; Fiscal Year: 2013
    ..using Fourier transform infrared spectroscopy, circular dichroism, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, native sodium dodecyl sulfate-polyacrylamide gel electrophoresis, matrix-assisted laser desorption/..
    Zbigniew Darzynkiewicz; Fiscal Year: 2013
    ..or ATM, Chk2 and p53 phosphorylation) with the measurement of actual DNA damage, assessed by single-cell DNA electrophoresis (comet assay)...
  8. Mechanisms and metabolic implications of LRP130 in NAFLD
    Marcus P Cooper; Fiscal Year: 2013
    ..We will evaluate respiratory chain supercomplexes using blue native gel electrophoresis. Mitochondrial function will be measured by oxygen consumption, complex activity, membrane potential and ..
  9. Thiol Isomerases During Thrombus Formation
    Barbara C Furie; Fiscal Year: 2013
    ..and endothelial cells will be labeled, the membranes purified, and these proteins separated by 2D gel electrophoresis using 16-BAC/SDS...
  10. Novel High Affinity and Long-Acting Bovine FSH Analogs for Veterinary Superovulat
    BRUCE DALE WEINTRAUB; Fiscal Year: 2012
    ..in vitro by previously validated bFSH analog ELISA immunoassay, robust in vitro bioassay, SDS-PAGE electrophoresis and isoelectric focusing gel analysis. 3...
    Helen A Baghdoyan; Fiscal Year: 2013
    ..collecting endogenously released neurotransmitters;2) high performance liquid chromatography and capillary electrophoresis to measure changes in endogenous neurotransmitters caused by antipsychotic drugs;and 3) polygraphic ..
  12. Identification and Validation of Alcohol Biomarker Signatures by Proteomics
    Emanuel Rubin; Fiscal Year: 2010
    ..A combination of 2D electrophoresis with multidimensional liquid chromatography and tandem mass spectroscopy will be employed to characterize ..
  13. Biophysics of Drug Interactions with Viral Episomes
    James K Bashkin; Fiscal Year: 2013
    ..The genome will be analyzed as overlapping 500 bp sequences using capillary electrophoresis and hydroxy radical footprinting...
  14. NeuroMolecular consequences of adolescent binge drinking
    Toni R Pak; Fiscal Year: 2013
    ..Aim 1 employs proteomic techniques, including 2-D fluorescence difference gel electrophoresis combined with electrospray ionization (MSn) mass spectrometry, to identify specific proteins that associate ..
  15. Validation and Quantification of FFPE Antigen Retrieval by Proteome Analysis
    XUPEING FANG; Fiscal Year: 2010
    ..sample amount constraints imposed by current proteome techniques including two-dimensional polyacrylamide gel electrophoresis and multidimensional liquid chromatography system, the lack of optimized methodologies for retrieving ..
  16. Biogenesis of lipoarabinomannan in mycobacteria
    Mary Jackson; Fiscal Year: 2013
    ..arabinomannan;AcylT, acyltransferase;Araf, arabinofuranosyl;AraT, arabinosyltransferase;CZE, capillary zone electrophoresis;DOC, deoxycholate;ESI, electrospray ionization;GT, glycosyltransferase;LAM, lipoarabinomannan;LM, lipomannan;..
    EunDuck P Kay; Fiscal Year: 2010
    ..transfection, multiplex protein array, GTP-pulldown assay, co-immunoprecipitation, two-dimensional gel electrophoresis, transcription assay, confocal microscopy, etc)...
  18. Analysis and Therapy of Age-Dependent Proteostasis Failure in Neurodegeneration
    ..Proteins from each fraction will be resolved by gel electrophoresis, and peptides will be cleaved by trypsin and analyzed by LC-MS2 to define and quantify the proteins and their ..
  19. Microanalytical methods for the detection of reactive nitrogen species
    Susan M Lunte; Fiscal Year: 2010
    ..and efficient separations of these transient species can be accomplished in less than a minute by microchip electrophoresis. In addition, detectors can be directly integrated into the chip format for the selective and sensitive ..
  20. Defensin-induced adaptive immunity to HagB
    Kim A Brogden; Fiscal Year: 2013
    ..and rFimA receptors on cell membranes will be identified using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE)...
  21. Global Proteomic Screening by MALDI Spectrometric Imaging of Protein-Bead Arrays
    Mark Lim; Fiscal Year: 2012
    ..methods used in conjunction with conventional mass spectrometry based proteomics such as two-dimensional gel electrophoresis and liquid chromatography are slow and not nearly as robust as the physical arraying/sorting of proteins ..
  22. Rapid Heart Attack Detection using an AC Electrokinetic Device
    Rajaram Krishnan; Fiscal Year: 2011
    ..will separate CFC-HMW DNA and EMPs directly from whole blood and then elute it for further analysis using gel electrophoresis, qRT-PCR and fluorescence analysis The specific aims for this Phase I application are: 1 - Use prototype ACE ..
    James F Rusling; Fiscal Year: 2013
    ..Capillary electrophoresis arrays will be used to identify specific sites of damage on key genes implicated in carcinogenesis...
  24. Multiplexed Detection of Food and Waterborne Pathogens
    Francis Barany; Fiscal Year: 2011
    ..The PCR/LDR assays will be migrated to the Cepheid GeneXpert system as well as other microfluidic devices initially using capillary electrophoresis or LDR-FRET, and subsequently Universal Array readout.
  25. Proteomics of Cell Death via 2-D Microfluidic Profiling
    DON DEVOE; Fiscal Year: 2009
    ..expertise in programmed cell death studies and bioinformatics with experience in the development of capillary electrophoresis, microfluidic, and mass spectrometry proteomic instrumentation. Dr. DeVoe (Univ...
  26. Functional Characterization of the Chlamydomonas CEP290/Nephrocystin-6 Homologue
    Branch Craige; Fiscal Year: 2010
    ..The protein composition of CEP290 mutant flagella will be compared to that of wild type using 2D gel electrophoresis and mass spectrometry...
    Andrew J W Huang; Fiscal Year: 2011
    ..responsible for bIGH3 gene activation in corneas will be characterized using in vivo UV photofootprinting and electrophoresis mobility shift assay (EMSA) along with deletion mutagenesis for confirmation...
  28. Contributions of Cardiac Myosin Binding Protein-C to Healthy and Failing Hearts
    CARL WEI CHAN TONG; Fiscal Year: 2013
    ..new skills of pressure-volume loop measurements to confirm HFpEF, 2-dimensional fluorescence difference gel electrophoresis to identify post-translation modifications on MyBPC3, stress technique to induce diastolic dysfunction in ..
  29. Prevention of diabetes by pDNA encoded with IL-10
    Jagdish Singh; Fiscal Year: 2010
    ..light scattering, electron microscopy, zeta potential measurement, UV spectrophotometer, titrimetric, and gel electrophoresis, respectively. (3)...
  30. Proteomics Biomarkers Development Laboratory
    Samir Hanash; Fiscal Year: 2009
    ..the current funding period in which a proteomics approach was utilized that consisted of two-dimensional gel electrophoresis to separate tumor cell and tumor tissue proteins followed by Western blotting and incubation of individual ..
    Frederick Southwick; Fiscal Year: 2010
    ..other proteins in the cell, the compensatory changes in other macrophage proteins will be assessed by 2-D gel electrophoresis and microsequencing, as well as by gene microarray analysis...
  32. Quantitative Mass Spectrometry Assays to Detect Multiple Myeloma and Assess Relap
    JOHN MATTHEW KOOMEN; Fiscal Year: 2010
    ..Current methods for protein biomarker detection are based on well established techniques, including gel electrophoresis and antibody-based detection...
  33. Single Cell Analysis of EGFR Activity in Human Airway Cells Exposed to Pollutants
    RYAN MATTHEW PHILLIPS; Fiscal Year: 2013
    ..addition of a phosphate group (phosphorylation), and then measuring the rate of this process using capillary electrophoresis, which allows the separation and measurement of molecules based on differences in size and charge...
  34. Protein Biomarkers of Gastric Cancer
    Victor E Reyes; Fiscal Year: 2010
    ..epithelial cells, whose proteins will be solubilized for profiling by high resolution two-dimensional gel electrophoresis (2-DE)...
  35. Engineering a packaging nanomotor for delivery of RNA and other molecules
    Venigalla B Rao; Fiscal Year: 2010
    ..and translocation into proheads is assessed by the presence of nuclease-protected RNA following agarose gel electrophoresis. The simplicity of this system lends itself well to the high-throughput format, allowing hundreds of mutant ..
  36. RpoS-mediated virulence regulation in Borrelia burgdorferi
    JON SCOTT BLEVINS; Fiscal Year: 2013
    ..microarray analyses (Specific Aim 1), in Specific Aim 2, we will use two-dimensional difference in-gel electrophoresis (2D-DIGE) to observe changes in the B...
  37. Sex Differences in Acute Estradiol Regulation of the Synaptic Proteome
    Catherine S Woolley; Fiscal Year: 2013
    ..In Aim 1, we will use two dimensional difference in gel electrophoresis followed by mass spectrometry to identify synaptic proteins that are differentially regulated by acute ER[..
  38. The Role of SaeR/S During Staphylococcus aureus Skin Infections
    Jovanka M Voyich; Fiscal Year: 2012
    ..aureus) is a major cause of human morbidity and mortality. Strains classified as pulsed-field gel electrophoresis type (PFGE) USA300 are particularly adept at causing skin and soft-tissue infections...
  39. Recognition of host catecholamines in Borrelia burgdorferi gene regulation
    Linden T Hu; Fiscal Year: 2010
    ..identify genes that are co-regulated with OspA in response to NE by gene array and two dimensional protein electrophoresis. We will identify the B...
  40. Establishment of a bioresource for discovery and evaluation of genetic and other
    Danish Saleheen; Fiscal Year: 2010
    ..700C) and to extract DNA from buffy coat and to check for quality control through genotyping and agarose gel electrophoresis;(ii) establishment of recruitment procedures that have already enabled recruitment of over 300 imaging ..
  41. Role of Elastolytic Cathepsins in Emphysema
    Harold A Chapman; Fiscal Year: 2010
    ..the identification of the biochemical "signature" of excess cathepsin S activity in mouse lung using 2D gel electrophoresis and mass spectroscopy , guided in part by identification of candidate genes in human emphysema through ..
  42. Non-invasive Assessment of Tissue Engineered Human Oral Mucosa
    Mary Ann Mycek; Fiscal Year: 2012
    ..the oral keratinocyte layer(s) of the EVPOME: SPECIFIC AIMS: (1) Test the efficacy of a microfluidic on-line electrophoresis immunoassay on a chip to assay three constitutively secreted proteins of oral keratinocytes important in ..
  43. Chiral Microchip Electrophoresis - Mass Spectrometric Methods for Metabolic Studi
    Yiming Liu; Fiscal Year: 2013
    ..To achieve the research goals, new chiral analytical methods based on microchip electrophoresis-tandem mass spectrometry (MCE-MS/MS) will be developed for high throughput chiral analysis of single cells...
  44. The Role of Plasmids in Rickettsia Biology
    ..We will use pulsed field gel electrophoresis and Southern blot techniques to define the distribution of plasmids within the genus...
  45. Role of Mechanisms of Efflux in Manganese Homeostasis and Detoxification
    Somshuvra Mukhopadhyay; Fiscal Year: 2013
    ..improve my technical skills during this period and gain proficiency in yeast-2-hybrid, 2 D fluorescence gel electrophoresis and genome-wide RNAi screening...
  46. Ultrasound-Assisted AQP1 Gene Therapy for Functional Restoration of Salivary Glan
    Michael J Passineau; Fiscal Year: 2013
    ..Scans will be performed using 2-dimensional difference gel electrophoresis, supplemented by multi- dimensional chromatography and mass spectrometry methods...
  47. SP-D as an immunomodulatory protein in acute rejection in lung transplantation
    KAREN D SIMS; Fiscal Year: 2010
    ..SNP beadchips and TaqMan assays will be used for SP-D genotyping, semi- quantitative native gel electrophoresis and Western blotting will measure SP-D structure, and RT-PCR will be used to measure macrophage polarization ..
  48. Regulation of Borealin Function by Mitotic Phosphorylation
    William Taylor; Fiscal Year: 2009
    ..Several activities of Borealin may be regulated by mitotic phosphorylation. Using electrophoresis to detect the mitotic phosphorylated form of Borealin we will analyze the impact of phosphorylation on some ..
    W Timothy Garvey; Fiscal Year: 2010
    ..muscle mitochrondrial mass and morphology, and to study respiratory chain proteomics using 2D blue native gel electrophoresis and mass spectrometry...
    MARIA R GONZALEZ BARO; Fiscal Year: 2012
    ..The nuclear receptors involved in GPAT2 regulation will be determined by super-shift-gel electrophoresis assays...
  51. Vaccinia Virus Genetics and Morphogenesis
    Richard C Condit; Fiscal Year: 2013
    ..structural proteins, and analyzing the virions and their degradation products by electron microscopy, gel electrophoresis and western blotting...
  52. Factors influencing regulation of the dynamics of the actin filament pointed end
    Alla S Kostyukova; Fiscal Year: 2013
    ..Binding will be assayed using native gel electrophoresis, cross-linking, and circular dichroism spectroscopy...
  53. Effects of saliva on herpes simplex virus infection of oralcells
    CLAUDE F KRUMMENACHER; Fiscal Year: 2013
    ..Identification will be performed by mass spectrometry, 2D-gel electrophoresis and biochemical assays on active purified fractions...
  54. Mechanisms of Polycomb Silencing
    Yi Zhang; Fiscal Year: 2010
    ..A 2D gel electrophoresis approach comparing affinity purified samples from paired cell lines with or without H2A ubiquitylation will ..
  55. Integrated Analyses of Taspase 1 (MLL Cleaving Protease)
    James J D Hsieh; Fiscal Year: 2010
    ..We will utilize 2-D difference in-gel electrophoresis in conjunction with mass spectrometry for the initial discovery, followed by phenotypic analyses of ..
  56. Risk for in vivo mutagenesis of the P53 gene by nucleoside analog antiviral drug
    Vernon E Walker; Fiscal Year: 2011
    ..PCR-based denaturing gradient gel electrophoresis, using psoralen-clamped primers, will be used to define the frequency and nature of mutations in p53 Exons 5-..
  57. Novel Reagents in Mouse Models of Autoimmune COPD
    Michael Borchers; Fiscal Year: 2012
    ..serum from CS-exposed mice, we will perform immunoprecipitation against lung antigens followed by 2D gel electrophoresis and mass spectroscopy analyses, and 2) Isolate autoreactive T cell clones that develop in response a mouse ..
  58. Cold Spring Harbor Laboratory Course on Proteomics
    David J Stewart; Fiscal Year: 2013
    ..gain hands-on experience in several quantitative proteome analysis methods, including two-dimensional gel electrophoresis and isotopic labeling strategies...
  59. Analysis of replication fork-collapse in yeast by site-specific DNA nicking
    RANJITH PRASAD ANAND; Fiscal Year: 2013
    ..Two-dimensional gel electrophoresis will be used to visualize replication fork progression across unrepaired nicks...
  60. Identification of gentamicin-binding proteins in the inner ear
    Takatoshi Karasawa; Fiscal Year: 2010
    ..We will use a gentamicin-agarose pull-down assay and SDS-polyacrylamide gel electrophoresis to isolate such proteins from kidney proximal tubule and cochlear cell lines, and determine their identity ..
  61. Regulation of PKR by Novel RNA Motifs
    Philip C Bevilacqua; Fiscal Year: 2012
    ..be assayed by chemical crosslinking and mutagenesis;and RNA tertiary structure will be assessed by native gel electrophoresis and structure mapping...
  62. MAVS protein: A role of mitochondria in innate immunity and cardioprotection
    Kirk Peterson; Fiscal Year: 2013
    ..adaptor molecules that connect MAVS to downstream innate defense mechanisms by 1) a proteomics approach using two-dimensional gel electrophoresis and mass spectrometry, and 2) the yeast two-hybrid screening method.
  63. Bio-imaging with Isothermal DNA Self-Assembly
    David Yu Zhang; Fiscal Year: 2013
    ..Isothermally assembled DNA nanostructures in both native and denaturing conditions have been verified by gel electrophoresis, atomic force microscopy, and total internal reflection fluorescence microscopy, and studies will shortly ..
  64. FMR 1-SLS: Improving Fragile X diagnosis using amplification-free single locus ta
    ..DNA fragment sizing methods are used today, either Southern Blotting or PCR followed by capillary electrophoresis, to categorize individuals as having normal, premutation or full mutation alleles...
  65. Cellular, molecular and functional characterization of proteasomes in EAE
    Oscar A Bizzozero; Fiscal Year: 2013
    ..the levels of each complex (20S, 11S- 20S-11S, 19S-20S-19S and 19S-20S-11S) by blue native-polyacrylamide gel electrophoresis and quantitative western blot analysis...
  66. Detection of HIV drug resistance mutations using highly multiplexed qPCR assays
    EUGENE SPIER; Fiscal Year: 2013
    ..Because capillary electrophoresis sequencing calls depend on the ability to detect "mixed bases" a mutation must represent at least 20% of the ..
  67. Development of a Laboratory Quality Management System for Food Testing to Meet th
    JACK R MORGAN; Fiscal Year: 2013
    ..The identification of the bacteria is completed by the use of serotyping and Pulsed Field Gel Electrophoresis (PFGE)...
    Stephen J Benkovic; Fiscal Year: 2013
    ..of chimeric fluorescent protein constructs, of co- immunoprecipitation, and of blue native polyacrylamide gel electrophoresis, the latter two linked to MALDI/MS/MS...
  69. Renewal of the UMDNJ NeuroProteomics Core Facility
    Hong Li; Fiscal Year: 2013
    ..A NINDS core grant will provide dedicated personnel proficient in sample preparation, 2D gel electrophoresis, mass spectrometry and bioinformatics, as well as instrument time and supplies associated with NINDS ..
    GORDON SHARP; Fiscal Year: 1990
    ..liquid chromatography ion, exchange, gel filtration, and immunoaffinity chromatography, and by gel electrophoresis. Antigens and autoantibodies will be vigorously characterized by immunological (Ouchterlony, passive ..
  71. FDA FERN Microbiological Cooperative Agreement Program Renewal
    PETE WILLIAM DOMBROSKI; Fiscal Year: 2013
    ..already in place such as traditional culture and biochemical identification, serotyping, and pulsed-field gel electrophoresis. The laboratory would use the resources from this grant to implement testing for additional organisms and to ..
  72. Interleukin and the Magnocellular Neuroendocrine System
    Joan Summy Long; Fiscal Year: 2005
    ..Using immunoaffinity capillary electrophoresis with laser-induced fluorescence detection, the quantitative relation-ships among IL-1beta, OT and VP in ..
  73. Multiplexed Single-Cell Analysis of EGFR and HER2 Activity in Gastric Cancer Cell
    ABIGAIL HALEY TURNER; Fiscal Year: 2013
    ..a single-cell analysis system comprised of a fluorescence microscope and an exquisitely sensitive capillary electrophoresis system with laser-induced fluorescence detection...
  74. PicoSep - A Microfluidic Single Molecule Free Solution Hydrodynamic Separation Pl
    Tza Huei Wang; Fiscal Year: 2013
    ..Platform Despite the power of modern methods such as microarrays, digital PCR, and deep sequencing, gel electrophoresis remains the workhorse of molecular biology...
  75. Modeling macromolecular transport through protein and solid-state nanopores
    Murugappan Muthukumar; Fiscal Year: 2013
    ..Chief among these is the nanopore-based electrophoresis. While excellent progress is continuously being made with this technique, there are many challenges in ..
  76. Two-Dimensional Microfluidic Platform for Rapid DNA Separation by Fragment Length
    LINDA B MC GOWN; Fiscal Year: 2013
    ..The specific aims of this proposal are to: (1) Use capillary gel electrophoresis to systematically investigate the DNA sequence selectivity of the G-gels and evaluate limits of sequence-..
  77. High throughput mass spectrometry and electrophoresis assay systems
    Robert T Kennedy; Fiscal Year: 2013
    ..Mass spectrometry (MS) and electrophoresis are highly promising as high-throughput analysis methods that can be performed with minimal or no ..
  78. Rapid Generation of High Affinity Protein Sensor Elements
    HYONGSOK SOH; Fiscal Year: 2009
    ..the partition efficiency (PE) of CMACS to be 1-2 orders of magnitude higher than those achieved by capillary electrophoresis (CE)...
  79. Diagonal Capillary Electrophoresis for Phosphorylation and Sialylation Analysis
    Norman J Dovichi; Fiscal Year: 2013
    Modified Abstract. This research group recently published the first description of diagonal capillary electrophoresis. Diagonal capillary electrophoresis is a form of two-dimensional capillary electrophoresis that employs identical ..
    DONNA KOSLOWSKY; Fiscal Year: 1999
    ..in analysis of spliceosomes and polyadenylation complexes will be employed as models, including: native gel electrophoresis, UV crosslinking, gel filtration and affinity chromatography...
  81. Microfluidic Selection of Aptamers
    Michael T Bowser; Fiscal Year: 2013
    ..We propose using micro free flow electrophoresis (?FFE) based selections to isolate high affinity aptamers for membrane proteins...
    Lawrence Chan; Fiscal Year: 1999
    ..The purified fractions will be analyzed by 2-dimensional gel electrophoresis and immunoblotting...
  83. Multi-Dimensional Separation of Bacteria
    G Scott Worthen; Fiscal Year: 2010
    ..design, build, and test a multidimensional, microfluidic sorter based on both structural (size-and shape and Electrophoresis) and functional (Adhesion, Chemotaxis) parameters to separate a complex bacterial mixture into bins ..
    Bela Asztalos; Fiscal Year: 2003
    ..in 12 apo A-I-containing subclasses of different size and charge, when subjected to two-dimensional gel electrophoresis immunoblot image analysis...
    Steven Nadler; Fiscal Year: 1993
    ..the potential to yield many more genetic markers than can be obtained by standard techniques such as allozyme electrophoresis. In addition, because DNA templates can be obtained from very small individuals, the RAPD technique may be ..
    Peter Law; Fiscal Year: 1992
    ..Survival and development of donor myoblasts in host muscles will be analyzed with electrophoresis and immunocytochemistry of muscle isozymes of glucose phosphate isomerase (GPI)...
  87. Synthesis and Evaluation of Nano-antioxidants for Medical Applications
    Choon Lee; Fiscal Year: 2009
    ..will be characterized by a number of chemical and bio-analytical techniques including polyacrylamide gel electrophoresis, HPLC, spectroscopy and mass spectrometry...
    Jin Chen; Fiscal Year: 2001
    ..Finally, a series of chemical cross-linking studies and gel filtration electrophoresis experiments will be employed to investigate the state of oligomerization of LERK-2 following stimulation of ..
  89. Quantitative Analysis of RING E3 Ubiquitin Ligases
    CHARLES M BRENNER; Fiscal Year: 2010
    ..By gel electrophoresis, multiple monoubiquitination and polyubiquitination both appear as ladders in which the target protein is ..
  90. DNA Sequencing at a Stretch
    Di Gao; Fiscal Year: 2009
    ..It will replace the electrophoresis-based technologies by a separation-at-a-stretch method to separate DNA strands in different lengths produced ..
  91. Molecular Micellar-Polyacrylamide Gel Electrophoresis (MoMi-PAGE)
    Isiah M Warner; Fiscal Year: 2010
    ..micelles to replace sodium dodecyl sulfate (SDS) and other conventional micelles in poly- acrylamide gel electrophoresis. The overall hypothesis of this research stems from our belief that molecular micelles should provide better ..