microdialysis

Summary

Summary: A technique for measuring extracellular concentrations of substances in tissues, usually in vivo, by means of a small probe equipped with a semipermeable membrane. Substances may also be introduced into the extracellular space through the membrane.

Top Publications

  1. ncbi Increase in muscle nociceptive substances and anaerobic metabolism in patients with trapezius myalgia: microdialysis in rest and during exercise
    Lars Rosendal
    National Institute of Occupational Health, Lersø Parkallé 105, DK 2100 Copenhagen, Denmark
    Pain 112:324-34. 2004
  2. ncbi Synaptic activity regulates interstitial fluid amyloid-beta levels in vivo
    John R Cirrito
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Neuron 48:913-22. 2005
  3. ncbi In vivo microdialysis reveals age-dependent decrease of brain interstitial fluid tau levels in P301S human tau transgenic mice
    Kaoru Yamada
    Department of Neurology, Hope Center for Neurological Disorders, Washington University, St Louis, Missouri 63110, USA
    J Neurosci 31:13110-7. 2011
  4. ncbi Microdialysis evaluation of atomoxetine brain penetration and central nervous system pharmacokinetics in rats
    William Kielbasa
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    Drug Metab Dispos 37:137-42. 2009
  5. ncbi Blood-brain barrier permeability after gamma whole-body irradiation: an in vivo microdialysis study
    Michel Diserbo
    Can J Physiol Pharmacol 80:670-8. 2002
  6. ncbi Active-site concentrations of chemicals - are they a better predictor of effect than plasma/organ/tissue concentrations?
    Margareta Hammarlund-Udenaes
    Division of Pharmacokinetics and Drug Therapy, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden
    Basic Clin Pharmacol Toxicol 106:215-20. 2010
  7. ncbi Acute focal neurological deficits in aneurysmal subarachnoid hemorrhage: relation of clinical course, CT findings, and metabolite abnormalities monitored with bedside microdialysis
    Asita Sarrafzadeh
    Department of Neurosurgery, Virchow Medical Clinic, Humboldt University of Berlin, Berlin, Germany
    Stroke 34:1382-8. 2003
  8. pmc Neuronal activity regulates the regional vulnerability to amyloid-β deposition
    Adam W Bero
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri, USA
    Nat Neurosci 14:750-6. 2011
  9. ncbi In vivo assessment of brain interstitial fluid with microdialysis reveals plaque-associated changes in amyloid-beta metabolism and half-life
    John R Cirrito
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci 23:8844-53. 2003
  10. pmc The effects of the cyclosporin A, a P-glycoprotein inhibitor, on the pharmacokinetics of baicalein in the rat: a microdialysis study
    T H Tsai
    National Research Institute of Chinese Medicine, 115 1 Li Nong Street Section 2, Shih Pai, Taipei 112, Taiwan
    Br J Pharmacol 137:1314-20. 2002

Detail Information

Publications392 found, 100 shown here

  1. ncbi Increase in muscle nociceptive substances and anaerobic metabolism in patients with trapezius myalgia: microdialysis in rest and during exercise
    Lars Rosendal
    National Institute of Occupational Health, Lersø Parkallé 105, DK 2100 Copenhagen, Denmark
    Pain 112:324-34. 2004
    ..Interstitial serotonin (5-HT), glutamate, lactate, pyruvate, and blood flow were determined by microdialysis in the trapezius muscle. Baseline pressure pain threshold (PPT) was lower (143+/-18 (TM) vs...
  2. ncbi Synaptic activity regulates interstitial fluid amyloid-beta levels in vivo
    John R Cirrito
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Neuron 48:913-22. 2005
    ..Abeta aggregation is concentration dependent and brain region specific. Utilizing in vivo microdialysis concurrently with field potential recordings, we demonstrate that Abeta levels in the brain interstitial fluid ..
  3. ncbi In vivo microdialysis reveals age-dependent decrease of brain interstitial fluid tau levels in P301S human tau transgenic mice
    Kaoru Yamada
    Department of Neurology, Hope Center for Neurological Disorders, Washington University, St Louis, Missouri 63110, USA
    J Neurosci 31:13110-7. 2011
    ..To address these issues, we developed a microdialysis technique to analyze monomeric ISF tau levels within the hippocampus of awake, freely moving mice...
  4. ncbi Microdialysis evaluation of atomoxetine brain penetration and central nervous system pharmacokinetics in rats
    William Kielbasa
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    Drug Metab Dispos 37:137-42. 2009
    ..and central nervous system (CNS) pharmacokinetics of atomoxetine in rats was conducted using brain microdialysis. We sought to determine the nature and extent of transport at the blood-brain barrier (BBB) and blood-..
  5. ncbi Blood-brain barrier permeability after gamma whole-body irradiation: an in vivo microdialysis study
    Michel Diserbo
    Can J Physiol Pharmacol 80:670-8. 2002
    ..barrier (BBB) to [3H]alpha-aminoisobutyric acid (AIBA) and [14C]sucrose were investigated using the microdialysis technique. Seven days, 3 and 6 weeks, and 3, 5, and 8 months after gamma exposure at a dose of 4...
  6. ncbi Active-site concentrations of chemicals - are they a better predictor of effect than plasma/organ/tissue concentrations?
    Margareta Hammarlund-Udenaes
    Division of Pharmacokinetics and Drug Therapy, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden
    Basic Clin Pharmacol Toxicol 106:215-20. 2010
    ..However, with the introduction of microdialysis and subsequently developed techniques, it has become possible to test the free drug hypothesis...
  7. ncbi Acute focal neurological deficits in aneurysmal subarachnoid hemorrhage: relation of clinical course, CT findings, and metabolite abnormalities monitored with bedside microdialysis
    Asita Sarrafzadeh
    Department of Neurosurgery, Virchow Medical Clinic, Humboldt University of Berlin, Berlin, Germany
    Stroke 34:1382-8. 2003
    ..ir)reversible neurological deficits and cerebral infarction in subarachnoid hemorrhage (SAH) patients by using the microdialysis technique and (2) to evaluate the influence of intracerebral hemorrhage (ICH) on microdialysis parameters.
  8. pmc Neuronal activity regulates the regional vulnerability to amyloid-β deposition
    Adam W Bero
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri, USA
    Nat Neurosci 14:750-6. 2011
    ..Using in vivo microdialysis, we show that ISF Aβ concentrations in several brain regions of APP transgenic mice before plaque deposition ..
  9. ncbi In vivo assessment of brain interstitial fluid with microdialysis reveals plaque-associated changes in amyloid-beta metabolism and half-life
    John R Cirrito
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Neurosci 23:8844-53. 2003
    ..Because assessment of ISF Abeta levels may provide unique insights into Abeta metabolism and AD, an in vivo microdialysis technique was developed to measure it...
  10. pmc The effects of the cyclosporin A, a P-glycoprotein inhibitor, on the pharmacokinetics of baicalein in the rat: a microdialysis study
    T H Tsai
    National Research Institute of Chinese Medicine, 115 1 Li Nong Street Section 2, Shih Pai, Taipei 112, Taiwan
    Br J Pharmacol 137:1314-20. 2002
    ..2. Three microdialysis probes were simultaneously inserted into the jugular vein, the hippocampus and the bile duct of male Sprague-..
  11. pmc Inhibition of P-glycoprotein enhances transport of imipramine across the blood-brain barrier: microdialysis studies in conscious freely moving rats
    F E O'Brien
    Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
    Br J Pharmacol 166:1333-43. 2012
    ..Therefore, the present study aimed to investigate the effect of P-gp inhibition on the transport of the tricyclic antidepressant imipramine and its active metabolite desipramine across the BBB...
  12. ncbi Muscle oxygenation and glycolysis in females with trapezius myalgia during stress and repetitive work using microdialysis and NIRS
    Gisela Sjøgaard
    Institute of Sport Sciences and Clinical Biomechanics, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark
    Eur J Appl Physiol 108:657-69. 2010
    ..At rest, during PEG, and STR the trapezius muscle was measured using (1) EMG and MMG, (2) microdialysis, and (3) NIRS. Further, subjective pain ratings were scored (VAS)...
  13. ncbi Role of bedside microdialysis in the diagnosis of cerebral vasospasm following aneurysmal subarachnoid hemorrhage
    A W Unterberg
    Department of Neurosurgery and Radiology, Charite, Humboldt University, Berlin, Germany
    J Neurosurg 94:740-9. 2001
    ..Cerebral online microdialysis monitoring may detect the metabolic changes in the extracellular fluid associated with ischemia...
  14. ncbi Interstitial muscle lactate, pyruvate and potassium dynamics in the trapezius muscle during repetitive low-force arm movements, measured with microdialysis
    L Rosendal
    National Institute of Occupational Health, Copenhagen, Denmark
    Acta Physiol Scand 182:379-88. 2004
    Local muscle metabolic responses to repetitive low-force contractions and to intense static contractions were studied by microdialysis in humans.
  15. ncbi P-Glycoprotein-mediated efflux of phenobarbital, lamotrigine, and felbamate at the blood-brain barrier: evidence from microdialysis experiments in rats
    Heidrun Potschka
    Department of Pharmacology, Toxicology, and Pharmacy, School of Veterinary Medicine, Bunteweg 17, D 30559, Hannover, Germany
    Neurosci Lett 327:173-6. 2002
    ..by verapamil on brain access of the AEDs phenobarbital, lamotrigine, and felbamate was investigated by in vivo microdialysis in rats...
  16. pmc Microdialysis: is it ready for prime time?
    J Clay Goodman
    Department of Pathology, Neurology and Neurosurgery, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Opin Crit Care 15:110-7. 2009
    This review highlights recent advances in cerebral microdialysis for investigational and clinical neurochemical monitoring in patients with critical neurological conditions.
  17. ncbi Translational neurochemical research in acute human brain injury: the current status and potential future for cerebral microdialysis
    Lars Hillered
    Division of Neurosurgery, Department of Surgery, The David Geffen UCLA School of Medicine, Los Angeles, California, USA
    J Neurotrauma 22:3-41. 2005
    b>Microdialysis (MD) was introduced as an intracerebral sampling method for clinical neurosurgery by Hillered et al. and Meyerson et al. in 1990...
  18. ncbi Uncovering the biochemical milieu of myofascial trigger points using in vivo microdialysis: an application of muscle pain concepts to myofascial pain syndrome
    Jay P Shah
    Rehabilitation Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1604 USA
    J Bodyw Mov Ther 12:371-84. 2008
    This article discusses muscle pain concepts in the context of myofascial pain syndrome (MPS) and summarizes microdialysis studies that have surveyed the biochemical basis of this musculoskeletal pain condition...
  19. ncbi The human brain utilizes lactate via the tricarboxylic acid cycle: a 13C-labelled microdialysis and high-resolution nuclear magnetic resonance study
    Clare N Gallagher
    Division of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge, UK
    Brain 132:2839-49. 2009
    ..We used a novel combination of (13)C-labelled cerebral microdialysis both to deliver (13)C substrates into the brain and recover (13)C metabolites from the brain, and high-..
  20. ncbi Microdialysis in the human brain and its potential role in the development and clinical assessment of drugs
    Adel Helmy
    University of Cambridge, Academic Unit of Neurosurgery, Box 167, Level 4, Block A, Addenbrooke s Hospital, Hills Road, Cambridge CB2 0QQ, UK
    Curr Med Chem 14:1525-37. 2007
    ..b>Microdialysis is an established, commercially available, clinical and research tool that is used to sample brain ..
  21. pmc The neuropharmacokinetics of temozolomide in patients with resectable brain tumors: potential implications for the current approach to chemoradiation
    Jana Portnow
    Department of Medical Oncology and Experimental Therapeutics, 1500 East Duarte Road, Duarte, CA 91010, USA
    Clin Cancer Res 15:7092-8. 2009
    Intracerebral microdialysis (ICMD) is an accepted method for monitoring changes in neurochemistry from acute brain injury...
  22. pmc Alteration in P-glycoprotein functionality affects intrabrain distribution of quinidine more than brain entry-a study in rats subjected to status epilepticus by kainate
    Stina Syvänen
    Division of Pharmacology, LACDR, Leiden University, The Netherlands
    AAPS J 14:87-96. 2012
    This study aimed to investigate the use of quinidine microdialysis to study potential changes in brain P-glycoprotein functionality after induction of status epilepticus (SE) by kainate...
  23. pmc The designer methcathinone analogs, mephedrone and methylone, are substrates for monoamine transporters in brain tissue
    Michael H Baumann
    Translational Pharmacology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA
    Neuropsychopharmacology 37:1192-203. 2012
    ..In vivo microdialysis in rat nucleus accumbens showed that i.v. administration of 0.3 and 1...
  24. ncbi Lactate levels in the brain are elevated upon exposure to volatile anesthetics: a microdialysis study
    Tobias Horn
    Department of Pharmacology, College of Pharmacy, Biocenter, Goethe University, Frankfurt am Main, Germany
    Neurochem Int 57:940-7. 2010
    ..In vivo-microdialysis was used to monitor extracellular energy metabolites in the brain of awake mice and during anesthesia with ..
  25. ncbi Cerebral microdialysis: research technique or clinical tool
    M M Tisdall
    Department of Neuroanaesthesia and Neurocritical Care, The National Hospital for Neurology and Neurosurgery Queen Square, London WC1N 3BG, UK
    Br J Anaesth 97:18-25. 2006
    Cerebral microdialysis is a well-established laboratory tool that is increasingly used as a bedside monitor to provide on-line analysis of brain tissue biochemistry during neurointensive care...
  26. ncbi Isoflurane anesthesia enhances the inhibitory effects of cocaine and GBR12909 on dopamine transporter: PET studies in combination with microdialysis in the monkey brain
    H Tsukada
    Central Research Laboratory, Hamamatsu Photonics K K, 5000 Hirakuchi, Hamakita, Japan
    Brain Res 849:85-96. 1999
    ..isoflurane-anesthetized monkeys using high-resolution positron emission tomography (PET) in combination with microdialysis. The striatal DAT availability and dopamine D(2) receptor binding were assayed with [11C]beta-CFT (WIN35,428) ..
  27. ncbi Peroxynitrite generated in the rat spinal cord induces neuron death and neurological deficits
    F Bao
    Department of Neurology, University of Texas Medical Branch, 301 University Boulevard RT 0653, Galveston, TX 77555 0653, USA
    Neuroscience 115:839-49. 2002
    ..Peroxynitrite was generated by administering 5 mM S-morpholinosydnonimine, a donor of peroxynitrite, through a microdialysis fiber into the gray matter of the rat spinal cord for 5 h...
  28. ncbi Assessment of blood flow changes in human skin by microdialysis urea clearance
    Simon Farnebo
    Department of Plastic Surgery, Hand Surgery, and Burns, Linkoping University Hospital, Linkoping University, Linkoping, Sweden
    Microcirculation 18:198-204. 2011
    ....
  29. ncbi Extracellular glutamate and other amino acids in experimental intracerebral hemorrhage: an in vivo microdialysis study
    Adnan I Qureshi
    Department of Neurosurgery, Toshiba Stroke Research Center, University of Buffalo, State University of New York, USA
    Crit Care Med 31:1482-9. 2003
    ....
  30. ncbi Stimulation of the anterior nucleus of the thalamus induces changes in amino acids in the hippocampi of epileptic rats
    Huan guang Liu
    Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No 6, Tian Tan Xi Li, Beijing 100050, China
    Brain Res 1477:37-44. 2012
    ..b>Microdialysis probes were unilaterally lowered into the CA3 region of the hippocampus, but probes were implanted bilaterally ..
  31. ncbi Extracellular serotonin level in the basolateral nucleus of the amygdala and dorsal periaqueductal gray under unconditioned and conditioned fear states: an in vivo microdialysis study
    Janaina M Zanoveli
    Instituto de Neurociências and Comportamento INeC, Campus USP, 14040 901 Ribeirao Preto, SP, Brazil
    Brain Res 1294:106-15. 2009
    ..acid (5-HIAA) in the BLA and dPAG during unconditioned and conditioned fear states using in vivo microdialysis procedure...
  32. ncbi Intestinal ischaemia during cardiac arrest and resuscitation: comparative analysis of extracellular metabolites by microdialysis
    Ulrike Korth
    Faculty of Clinical Medicine Mannheim, Institute of Anesthesiology, University of Heidelberg, 68165 Mannheim, Germany
    Resuscitation 58:209-17. 2003
    ..The present study was designed to use microdialysis as a tool to monitor intestinal ischaemia after cardiac arrest and resuscitation in pigs...
  33. ncbi Comparison of brain extracellular fluid, brain tissue, cerebrospinal fluid, and serum concentrations of antiepileptic drugs measured intraoperatively in patients with intractable epilepsy
    Bernhard Rambeck
    Biochemical Laboratory of the Epilepsy Research Foundation, University of Munster, Germany
    Epilepsia 47:681-94. 2006
    ..measure concentrations of AEDs in the extracellular space of human neocortical tissue by using intraoperative microdialysis (IOMD) in those parts of the brain that had to be removed for therapeutic reasons...
  34. ncbi Medial prefrontal cortex determines how stressor controllability affects behavior and dorsal raphe nucleus
    J Amat
    Department of Psychology, Campus Box 345, University of Colorado, Boulder, Colorado 80903 0345, USA
    Nat Neurosci 8:365-71. 2005
    ..This suggests a new function for the mPFCv and implies that the presence of control inhibits stress-induced neural activity in brainstem nuclei, in contrast to the prevalent view that such activity is induced by a lack of control...
  35. ncbi Effect of adenosine A(2A) receptor antagonists on L-DOPA-induced hydroxyl radical formation in rat striatum
    Krystyna Gołembiowska
    Institute of Pharmacology, Polish Academy of Sciences, 12 Smetna Street, Kraków 31 343, Poland
    Neurotox Res 15:155-66. 2009
    ..ZM 241385) on L: -3,4-dihydroxyphenylalanine (L: -DOPA)-induced hydroxyl radical generation using in vivo microdialysis in the striatum of freely moving rats...
  36. pmc Dynamic metabolic response to multiple spreading depolarizations in patients with acute brain injury: an online microdialysis study
    Delphine Feuerstein
    Department of Bioengineering, Imperial College London, London, UK
    J Cereb Blood Flow Metab 30:1343-55. 2010
    ..We here characterize the dynamic metabolic response to these events. A microdialysis catheter was inserted into perilesional cortical tissue adjacent to a strip for electrocorticography following ..
  37. ncbi The role of cortical and hypothalamic histamine-3 receptors in the modulation of central histamine neurotransmission: an in vivo electrophysiology and microdialysis study
    Gunnar Flik
    Department of Biomonitoring and Sensoring, Groningen Research Institute of Pharmacy GRIP, University of Groningen, Groningen, The Netherlands
    Eur J Neurosci 34:1747-55. 2011
    ..Extracellular histamine levels were determined using the dual (PFC and TMN) probe microdialysis, in freely-moving animals...
  38. ncbi Secretion of ghrelin from rat stomach ghrelin cells in response to local microinfusion of candidate messenger compounds: a microdialysis study
    Charlotta Dornonville de la Cour
    Unit of Cellular and Molecular Pharmacology, Dept of Exp Med, University of Lund, BMC F13, S 221 84 Lund, Sweden
    Regul Pept 143:118-26. 2007
    ..g. calcitonin, glucagon, insulin, PTH). Microdialysis probes were placed in the submucosa of the acid-producing part of the rat stomach...
  39. ncbi Use of rapid sampling microdialysis for intraoperative monitoring of bowel ischemia
    S Deeba
    Department of Biosurgery and Surgical Technology, St Mary s Hospital, Imperial College London, London, United Kingdom
    Dis Colon Rectum 51:1408-13. 2008
    ..This study used rapid sampling online microdialysis to evaluate the biochemical changes occurring in a segment of human bowel during and after resection, and ..
  40. pmc Local release of ATP into the arterial inflow and venous drainage of human skeletal muscle: insight from ATP determination with the intravascular microdialysis technique
    Stefan P Mortensen
    The Copenhagen Muscle Research Centre, Rigshospitalet, Section 7652, Blegdamsvej 9, DK 2100 Copenhagen Ø, Denmark
    J Physiol 589:1847-57. 2011
    ..into the mechanisms by which ATP is released into plasma, we measured plasma [ATP] with the intravascular microdialysis technique at rest and during dynamic exercise (normoxia and hypoxia), passive exercise, thigh compressions and ..
  41. ncbi Cerebral ischemia in aneurysmal subarachnoid hemorrhage: a correlative microdialysis-PET study
    Asita S Sarrafzadeh
    Charité Campus Virchow Medical Clinic, Humboldt University of Berlin, Augustenburger Platz 1, D 13353 Berlin, Germany
    Stroke 35:638-43. 2004
    Cerebral microdialysis (MD) is discussed as a technique for detection of cerebral ischemia in subarachnoid hemorrhage; however, clinical data on cerebral blood flow (CBF) are limited in these patients...
  42. ncbi Pubertal acceleration of pulsatile gonadotropin-releasing hormone release in male rats as revealed by microdialysis
    Glenn C Harris
    Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA
    Endocrinology 144:163-71. 2003
    A microdialysis technique was used in male rats to directly assess the postulate that pubertal maturation is associated with accelerated GnRH pulsatility...
  43. ncbi Persistent arterial hyperammonemia increases the concentration of glutamine and alanine in the brain and correlates with intracranial pressure in patients with fulminant hepatic failure
    Flemming Tofteng
    Department of Hepatology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
    J Cereb Blood Flow Metab 26:21-7. 2006
    ..By using in vivo brain microdialysis technique together with ICP monitoring in 17 FHF patients (10 females/7 males; median age 49 (range 18 to 66) ..
  44. ncbi Glucocorticoid receptor impairment alters CNS responses to a psychological stressor: an in vivo microdialysis study in transgenic mice
    A C Linthorst
    Max Planck Institute of Psychiatry, Section Neuropsychopharmacology, Kraepelinstrasse 2, D 80804 Munich, Germany
    Eur J Neurosci 12:283-91. 2000
    ..putative disturbances on the level of brain neurotransmission in GR-impaired (GR-i) mice using an in vivo microdialysis method...
  45. ncbi Effects of manganese on extracellular levels of dopamine in rat striatum: an analysis in vivo by brain microdialysis
    L Vidal
    Department Functional Biology and Health Sciences, University of Vigo, Campus As Lagoas Marcosende, 36200, Vigo, Spain
    Neurochem Res 30:1147-54. 2005
    ..The infusion of MnCl2 removed the increase in DA levels, without affecting DOPAC and HVA levels. Perfusion of NSD 1015 increased the extracellular levels of L-DOPA in striatum, and MnCl2 increased the effect of NSD1015 on L-Dopa...
  46. ncbi Association between elevated brain tissue glycerol levels and poor outcome following severe traumatic brain injury
    Tobias Clausen
    Department of Anesthesiology and Intensive Care Medicine, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    J Neurosurg 103:233-8. 2005
    ..Recently, it has become feasible to measure via microdialysis cerebral extracellular fluid (ECF) glycerol concentrations at the patient's bedside...
  47. ncbi Effect of cerebral perfusion pressure augmentation on regional oxygenation and metabolism after head injury
    Andrew J Johnston
    University Department of Anaesthetics, Addenbrooke s Hospital, Cambridge, UK
    Crit Care Med 33:189-95; discussion 255-7. 2005
    In this study we have used O positron emission tomography, brain tissue oxygen monitoring, and cerebral microdialysis to assess the effects of cerebral perfusion pressure augmentation on regional physiology and metabolism in the setting ..
  48. ncbi Opposite influences of endogenous dopamine D1 and D2 receptor activation on activity states and electrophysiological properties of striatal neurons: studies combining in vivo intracellular recordings and reverse microdialysis
    Anthony R West
    Department of Neuroscience, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Neurosci 22:294-304. 2002
    ..of striatal neurons in vivo was investigated by performing intracellular recordings concurrently with reverse microdialysis in chloral hydrate-anesthetized rats...
  49. ncbi Consensus meeting on microdialysis in neurointensive care
    Bo Michael Bellander
    Section of Neurosurgery, Department of Clinical Neuroscience, Karolinska Institutet and Hospital, Stockholm, Sweden
    Intensive Care Med 30:2166-9. 2004
    b>Microdialysis is used in many European neurointensive care units to monitor brain chemistry in patients suffering subarachnoid hemorrhage (SAH) or traumatic brain injury (TBI).
  50. pmc Locomotor stimulation produced by 3,4-methylenedioxymethamphetamine (MDMA) is correlated with dialysate levels of serotonin and dopamine in rat brain
    Michael H Baumann
    Clinical Psychopharmacology Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, United States
    Pharmacol Biochem Behav 90:208-17. 2008
    ..Male rats undergoing in vivo microdialysis were housed in chambers equipped with photobeams for measurement of ambulation (i.e...
  51. ncbi Biochemical alterations in the trapezius muscle of patients with chronic whiplash associated disorders (WAD)--a microdialysis study
    Björn Gerdle
    Division of Rehabilitation Medicine, Department of Neuroscience and Locomotion, Faculty of Health Sciences, SE 58185 Linkoping, Sweden
    Eur J Pain 12:82-93. 2008
    ..pyruvate, potassium, interleukin-6 (IL-6), and blood flow were determined in the trapezius muscle using a microdialysis technique. Pressure pain thresholds (PPT) over trapezius and tibialis anterior muscles were also assessed...
  52. ncbi Baseline levels of glucose metabolites, glutamate and glycerol in malignant glioma assessed by stereotactic microdialysis
    Michael Roslin
    Department of Neurosurgery, Umea University Hospital, Umea, Sweden
    J Neurooncol 61:151-60. 2003
    The metabolism of high grade astrocytoma was studied in 15 patients using intra-tumoural microdialysis. Two catheters were implanted during a stereotactic biopsy procedure: one in the tumour and one in the peri-tumoural tissue...
  53. ncbi A microdialysis method for the recovery of IL-1beta, IL-6 and nerve growth factor from human brain in vivo
    Craig D Winter
    Neurosurgical Department, Wessex Neurological Centre, Southampton General Hospital, Southampton SO16 6YD, UK
    J Neurosci Methods 119:45-50. 2002
    Intracerebral microdialysis is used extensively as a research tool in the investigation of the neurochemical and metabolic changes that occur following acute brain injury...
  54. ncbi Norepinephrine mediates glucoprivic-induced increase in GABA in the ventromedial hypothalamus of rats
    J L Beverly
    Department of Animal Sciences, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Am J Physiol Regul Integr Comp Physiol 279:R990-6. 2000
    ..These results support an interaction among noradrenergic and GABAergic systems in the VMN during glucoprivation and that increased NE mediates the increase in extracellular GABA after 2-DG...
  55. pmc Social context-dependent singing-regulated dopamine
    Aya Sasaki
    Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Neurosci 26:9010-4. 2006
    ..Here we show in the first report of in vivo microdialysis in awake, behaving songbirds that singing is associated with increased dopamine levels in Area X...
  56. ncbi In vivo assessment of high-grade glioma biochemistry using microdialysis: a study of energy-related molecules, growth factors and cytokines
    Hani J Marcus
    Division of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    J Neurooncol 97:11-23. 2010
    b>Microdialysis enables measurement of the chemistry of the cerebral extracellular fluid...
  57. ncbi The mechanism of 1,2,3,4-tetrahydroisoquinolines neuroprotection: the importance of free radicals scavenging properties and inhibition of glutamate-induced excitotoxicity
    Lucyna Antkiewicz-Michaluk
    Department of Biochemistry, Polish Academy of Sciences, Cracow, Poland
    J Neurochem 97:846-56. 2006
    ..Finally, we demonstrated in an in vivo microdialysis experiment that 1MeTIQ prevents kainate-induced release of excitatory amino acids from the rat frontal cortex...
  58. ncbi New perspectives from microdialysis studies in freely-moving, spontaneously hypertensive rats on the pharmacology of drugs for the treatment of ADHD
    D J Heal
    RenaSci Consultancy Limited, BioCity, Nottingham NG1 1GF, UK
    Pharmacol Biochem Behav 90:184-97. 2008
    ..In vivo microdialysis in freely-moving rats has been employed extensively to provide a better understanding of the pharmacodynamics ..
  59. ncbi Plasma, tumor, and tissue disposition of STEALTH liposomal CKD-602 (S-CKD602) and nonliposomal CKD-602 in mice bearing A375 human melanoma xenografts
    William C Zamboni
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Clin Cancer Res 13:7217-23. 2007
    ..For STEALTH liposomal formulations of anticancer agents to achieve antitumor effects, the active drug must be released into the tumor extracellular fluid (ECF)...
  60. ncbi Temporal profile of release of interleukin-1beta in neurotrauma
    K Fassbender
    Department of Neurology, University Clinic Mannheim, University of Heidelberg, Theodor Kutzer Ufer 1 3, D 68135, Mannheim, Germany
    Neurosci Lett 284:135-8. 2000
    ..In brain tissue perfusates obtained by in vivo microdialysis a marked release of IL-1beta was unexpectedly detected within less than 60 min...
  61. ncbi MK801 suppresses the L-DOPA-induced increase of glutamate in striatum of hemi-Parkinson rats
    Nadine Jonkers
    Department of Pharmaceutical Chemistry and Drug Analysis, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Brain Res 926:149-55. 2002
    In vivo microdialysis in freely moving rats was used to investigate the influence of the indirect dopamine receptor agonist levodopa (L-DOPA), alone and combined with the N-methyl-D-aspartate (NMDA) receptor antagonist dizocilpine (MK801)..
  62. ncbi Extracellular glutamate and other metabolites in and around RG2 rat glioma: an intracerebral microdialysis study
    P F Behrens
    Department of Neurology, Albert Ludwigs Universitat Freiburg, Germany
    J Neurooncol 47:11-22. 2000
    ..After confirmation of tumor growth and localization by contrast enhanced MRI three microdialysis probes were implanted simultaneously in the cortex: at the tumor implantation site (tumor), 2 mm caudally, ..
  63. ncbi Cerebral extracellular chemistry and outcome following traumatic brain injury: a microdialysis study of 223 patients
    Ivan Timofeev
    Division of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    Brain 134:484-94. 2011
    ..Monitoring of cerebral extracellular chemistry with microdialysis has the potential for early detection of metabolic derangements associated with such events...
  64. ncbi Splanchnic metabolism during gut ischemia and short-term endotoxin and hemorrhagic shock as evaluated by intravasal microdialysis
    Tobias Backström
    Department of Thoracic Surgery, Karolinska Hospital, Stockholm, Sweden
    Shock 21:572-8. 2004
    ..a model where shock-induced metabolic changes in the splanchnic region were studied by the use of intravasal microdialysis. 23 anesthetized domestic pigs were randomized into four groups: Group I, serving as controls (n = 5); Group ..
  65. ncbi Determination of phthalate esters from food-contacted materials by on-line microdialysis and liquid chromatography
    Jen Fon Jen
    Department of Chemistry, National Chung Hsing University, Taichung 40217, Taiwan
    J Chromatogr A 1130:28-33. 2006
    ..released from food-contacted plastics into aqueous solution during microwave conditioning were measured with microdialysis enrichment on-lined high-performance liquid chromatography...
  66. pmc Microdialysis with radiometric monitoring of L-[β-11C]DOPA to assess dopaminergic metabolism: effect of inhibitors of L-amino acid decarboxylase, monoamine oxidase, and catechol-O-methyltransferase on rat striatal dialysate
    Maki Okada
    Molecular Probe Group, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan
    J Cereb Blood Flow Metab 31:124-31. 2011
    ..To measure dopaminergic metabolism, we used microdialysis with radiometric detection to monitor L-[β-(11)C]DOPA metabolites in the extracellular space of the rat ..
  67. ncbi Sampling glutamate and GABA with microdialysis: suggestions on how to get the dialysis membrane closer to the synapse
    K L Drew
    Department of Chemistry and Biochemistry and Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, PO Box 757000, AK 99775 7000, USA
    J Neurosci Methods 140:127-31. 2004
    b>Microdialysis is currently optimized to sample the extrasynaptic pool...
  68. ncbi Regional analyses of CNS microdialysate glucose and lactate in seizure patients
    Eain M Cornford
    Department of Neurology, UCLA School of Medicine, Los Angeles, California 90073, USA
    Epilepsia 43:1360-71. 2002
    ..To correlate glucose (and lactate) results obtained from microdialysate to recent studies suggesting that glucose transporter activity may be significantly altered in seizures...
  69. ncbi Use of osmotic agents in microdialysis studies to improve the recovery of macromolecules
    William J Trickler
    Department of Pharmaceutical Sciences, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE 68198 6025, USA
    J Pharm Sci 92:1419-27. 2003
    The availability of microdialysis probes with higher molecular weight cutoff membranes has made it possible to measure larger molecular weight compounds like bioactive peptides and proteins...
  70. ncbi Chronic hyperammonemia induces tonic activation of NMDA receptors in cerebellum
    Nisrin ElMlili
    Laboratory of Neurobiology, Centro de Investigacion Principe Felipe, Valencia, Spain
    J Neurochem 112:1005-14. 2010
    ..MK-801 also increases nNOS in synaptic membranes and reduces it in cytosol. This indicates that hyperammonemia increases tonic activation of NMDA receptors leading to reduced activity of nNOS and of the glutamate--NO--cGMP pathway...
  71. ncbi Continuous monitoring of cerebral metabolism in traumatic brain injury: a focus on cerebral microdialysis
    Lars Hillered
    Department of Neuroscience, Uppsala University Hospital, Uppsala, Sweden
    Curr Opin Crit Care 12:112-8. 2006
    This review highlights recent advances in cerebral microdialysis as a tool for neurochemical monitoring of patients with traumatic brain injury.
  72. ncbi Microdialysis microneedles for continuous medical monitoring
    Jeffrey D Zahn
    Berkeley Sensor and Actuator Center, University of California, Berkeley, 497 Cory Hall, Berkeley, CA 94720 1774, USA
    Biomed Microdevices 7:59-69. 2005
    ..A novel microdialysis microneedle is introduced that is capable of excluding large MW compounds based on size...
  73. ncbi Extracellular brain ammonia levels in association with arterial ammonia, intracranial pressure and the use of albumin dialysis devices in pigs with acute liver failure
    Kerstin Zwirner
    Department of General, Visceral and Transplant Surgery, Tuebingen University Hospital, Tuebingen, Germany
    Metab Brain Dis 25:407-12. 2010
    ..All pigs were monitored and treated under intensive care conditions until death. Arterial blood and cerebral microdialysis samples were collected and ICP data recorded...
  74. ncbi Opioid-induced mast cell activation and vascular responses is not mediated by mu-opioid receptors: an in vivo microdialysis study in human skin
    James A Blunk
    Department of Anesthesiology, University Hospital, Erlangen, Germany
    Anesth Analg 98:364-70, table of contents. 2004
    ..In this study, we used intradermal microdialysis to assess whether this stimulatory effect of opioids on mast cells depends on the activation of opioid ..
  75. ncbi A method to evaluate the diffusion rate of drugs from a microdialysis probe through brain tissue
    B H Westerink
    University Centre for Pharmacy, Department of Biomonitoring and Sensoring, University of Groningen, Deusinglaan 1, 9713 AV, Groningen, The Netherlands
    J Neurosci Methods 109:53-8. 2001
    For interpretation of microdialysis experiments in which compounds are applied retrodialysis, it is important to have information about the migration rate of the infused compounds...
  76. ncbi Microdialysis-based analysis of interstitial NO in situ: NO synthase-independent NO formation during myocardial ischemia
    Claus Martin
    Institut fur Pathophysiologie, Zentrum für Innere Medizin des Universitätsklinikums, Essen, Germany
    Cardiovasc Res 74:46-55. 2007
    ..The contribution of NOS-independent pathways to NO synthesis was studied using NOS blockade...
  77. ncbi Tolcapone enhances food-evoked dopamine efflux and executive memory processes mediated by the rat prefrontal cortex
    C C Lapish
    Department of Psychiatry, University of British Columbia, 2255 Wesbrook Mall, Vancouver BCV6T2A1, Canada
    Psychopharmacology (Berl) 202:521-30. 2009
    ..Furthermore, it has been shown previously that COMT inhibitors increase pharmacologically evoked DA but do not affect basal levels in the PFC...
  78. ncbi In vivo induction of P-glycoprotein expression at the mouse blood-brain barrier: an intracerebral microdialysis study
    Gary N Y Chan
    Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario, Canada
    J Neurochem 127:342-52. 2013
    Intracerebral microdialysis was utilized to investigate the effect of P-glycoprotein (a drug efflux transporter) induction at the mouse blood-brain barrier (BBB) on brain extracellular fluid concentrations of quinidine, an established ..
  79. ncbi AAPS-FDA Workshop White Paper: microdialysis principles, application, and regulatory perspectives
    Chandra S Chaurasia
    Division of Bioequivalence, Office of Generic Drugs, Food and Drug Administration, Room 1360 HFD 650, 7520 Standish Place, Rockville, MD 20855, USA
    J Clin Pharmacol 47:589-603. 2007
  80. ncbi Prostaglandin release by spinal cord injury mediates production of hydroxyl radical, malondialdehyde and cell death: a site of the neuroprotective action of methylprednisolone
    D Liu
    Departments of Neurology and Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas, USA
    J Neurochem 77:1036-47. 2001
    ..The concentration of PGF(2alpha) measured by microdialysis sampling increased threefold immediately following impact injury to the rat spinal cord...
  81. pmc Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans
    H Langberg
    Sports Medicine Research Unit, Department of Rheumatology H, Bispebjerg Hospital, Copenhagen, Denmark
    J Physiol 515:919-27. 1999
    ..These processes are important for understanding the development of overuse inflammation and injury. 2. A microdialysis technique was used to determine interstitial concentrations of glycerol, glucose, lactate, prostaglandin E2 (..
  82. pmc Enhanced human tissue microdialysis using hydroxypropyl-ß-cyclodextrin as molecular carrier
    Marcus May
    Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany
    PLoS ONE 8:e60628. 2013
    b>Microdialysis sampling of lipophilic molecules in human tissues is challenging because protein binding and adhesion to the membrane limit recovery...
  83. ncbi Quinidine as an ABCB1 probe for testing drug interactions at the blood-brain barrier: an in vitro in vivo correlation study
    István Sziráki
    Laboratory of Microdialysis, SOLVO Biotechnology, Berlini u 47 49, Budapest, Hungary
    J Biomol Screen 16:886-94. 2011
    ..Brain exposure to quinidine was tested in dual-/triple-probe microdialysis experiments in rats by assessing levels of quinidine in blood and brain...
  84. ncbi BBB transport and P-glycoprotein functionality using MDR1A (-/-) and wild-type mice. Total brain versus microdialysis concentration profiles of rhodamine-123
    E C de Lange
    Leiden Amsterdam Center for Drug Research, Division of Pharmacology, Leiden University, The Netherlands
    Pharm Res 15:1657-65. 1998
    ..The effect of P-glycoprotein (Pgp) on brain distribution using mdr1a (-/-) mice was investigated...
  85. pmc Lactate uptake by the injured human brain: evidence from an arteriovenous gradient and cerebral microdialysis study
    Ibrahim Jalloh
    1 Division of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom
    J Neurotrauma 30:2031-7. 2013
    ..We recruited 19 patients with diffuse TBI, monitored with cerebral microdialysis and jugular bulb catheters...
  86. ncbi Verification of brain penetration of the unbound fraction of a novel HER2/EGFR dual kinase inhibitor (TAK-285) by microdialysis in rats
    Franciska Erdo
    Laboratory of Microdialysis, SOLVO Biotechnology, Berlini u 47 49, Budapest H 1045, Hungary
    Brain Res Bull 87:413-9. 2012
    ..the present work was to verify the presence of unbound TAK-285 in the rat brain after oral administration by a microdialysis technique with simultaneous sampling of blood and brain...
  87. ncbi Microdialysis of cytokines: methodological considerations, scanning electron microscopy, and determination of relative recovery
    Adel Helmy
    Division of Neurosurgery, Department of Clinical Neurosciences, University of Cambridge, Addenbrooke s Hospital, Cambridge, United Kingdom
    J Neurotrauma 26:549-61. 2009
    Cerebral microdialysis is a monitoring technique with expanding clinical and research utility following traumatic brain injury...
  88. pmc A qualitative and quantitative proteomic study of human microdialysate and the cutaneous response to injury
    Carolyn Gill
    Centre for Proteomic Research, School of Biological Sciences, University of Southampton, UK
    AAPS J 13:309-17. 2011
    ..Sampling of this compartment using continuous microdialysis allows assessment of the temporal changes in extracellular mediators involved in tissue homeostasis and ..
  89. ncbi Exploratory translational modeling approach in drug development to predict human brain pharmacokinetics and pharmacologically relevant clinical doses
    W Kielbasa
    Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 46285, USA
    Drug Metab Dispos 40:877-83. 2012
    ..In this study, we explored the potential utility of this translational approach using rat brain microdialysis and PK modeling techniques to predict human brain extracellular fluid PK of atomoxetine and duloxetine...
  90. ncbi Measurement of the pharmacokinetics and pharmacodynamics of neuroactive compounds
    Mohammad S Alavijeh
    Pharmidex Pharmaceutical Services Ltd, 72 New Bond Street, London, W1S 1RR, UK
    Neurobiol Dis 37:38-47. 2010
    ..In vivo microdialysis provides a powerful method for the measurement of endogenous and exogenous substances in the ISF surrounding ..
  91. ncbi Prostacyclin treatment in severe traumatic brain injury: a microdialysis and outcome study
    Magnus Olivecrona
    Department of Neurosurgery, Umea University Hospital, Umea, Sweden
    J Neurotrauma 26:1251-62. 2009
    ..This study is a prospective consecutive double-blinded randomized study on the effect of PGI(2) versus placebo in severe traumatic brain injury (sTBI). All patients with sTBI were eligible...
  92. ncbi Peroxynitrite generated in the rat spinal cord induces apoptotic cell death and activates caspase-3
    F Bao
    Department of Neurology, University of Texas Medical Branch, 301 University Boulevard RT 0653, Galveston, TX 77555 0653, USA
    Neuroscience 116:59-70. 2003
    ..concentration and duration produced by spinal cord injury by administering S-morpholinosydnonimine through a microdialysis fiber into the gray matter of the rat spinal cord...
  93. ncbi Metabolic markers obtained by microdialysis can detect secondary intestinal ischemia: an experimental study of ischemia in porcine intestinal segments
    Hanne Birke-Sorensen
    Department of Plastic Surgery, Institute of Clinical Medicine, Aarhus University Hospital, Nørrebrogade 44, 8000, Aarhus, Denmark
    World J Surg 34:923-32. 2010
    ..The aim of this study was to investigate the value of microdialysis (MD) as a monitoring method for detecting ischemia in intestinal transplants.
  94. ncbi Striatal NTS1 , dopamine D2 and NMDA receptor regulation of pallidal GABA and glutamate release--a dual-probe microdialysis study in the intranigral 6-hydroxydopamine unilaterally lesioned rat
    Luca Ferraro
    Department of Clinical and Experimental Medicine, Pharmacology Section and LTTA Centre, University of Ferrara, Via Fossato di Mortara 17 19, 44100 Ferrara, Italy
    Eur J Neurosci 35:207-20. 2012
    The current microdialysis study elucidates a functional interaction between the striatal neurotensin NTS(1) receptor and the striatal dopamine D(2) and N-methyl-d-aspartic acid (NMDA) receptors in the regulation of striatopallidal gamma-..
  95. ncbi Identification of metabolites of crude and processed Fructus Corni in rats by microdialysis sampling coupled with electrospray ionization linear quadrupole ion trap mass spectrometry
    Gang Cao
    Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 310053, PR China
    J Pharm Biomed Anal 56:118-25. 2011
    A microdialysis (MD) sampling coupled with electrospray ionization linear quadrupole ion trap mass spectrometry (LTQ-MS(n)) method has been developed for rapid and sensitive analysis of rat microdialysate metabolite profile of Fructus ..
  96. ncbi A longitudinal study of 5-HT outflow during chronic fluoxetine treatment using a new technique of chronic microdialysis in a highly emotional mouse strain
    Daniela Popa
    Univ Paris Sud, EA 3544, Fac Pharmacie, Chatenay Malabry Cedex, France
    Eur J Pharmacol 628:83-90. 2010
    ..The function of the 5-HT system in the raphe nuclei and hippocampus, was assessed by using repeated in vivo microdialysis sessions in awake freely moving mice, then studying its relation with behavior, analyzed mainly with open ..
  97. pmc Effect of adenosine A(2A) receptor antagonists and L-DOPA on hydroxyl radical, glutamate and dopamine in the striatum of 6-OHDA-treated rats
    Krystyna Gołembiowska
    Institute of Pharmacology, Polish Academy of Sciences, 12 Smetna Street, 31 343 Krakow, Poland
    Neurotox Res 21:222-30. 2012
    ..ZM 241385) on hydroxyl radical generation, and glutamate (GLU) and dopamine (DA) extracellular level using a microdialysis in the striatum of 6-OHDA-treated rats...
  98. ncbi Change in brain glucose after enteral nutrition in subarachnoid hemorrhage
    Kosaku Kinoshita
    Division of Emergency and Critical Care Medicine, Department of Acute Medicine, Nihon University School of Medicine, Itabashi, Tokyo
    J Surg Res 162:221-4. 2010
    ..In this study, we used brain microdialysis methods to estimate whether the physiologic elevation of plasma glucose following EN affects brain glucose ..
  99. ncbi Brain microdialysis in exercise research
    R Meeusen
    Department of Human Physiology and Sports Medicine, Faculty of Physical Education and Physiotherapy, Vrije Universiteit Brussel, Belgium
    Sports Med 31:965-83. 2001
    During the last 5 to 10 years, the microdialysis technique has been used to explore neurotransmitter release during exercise...
  100. ncbi Neurotensin regulates cortical glutamate transmission by modulating N-methyl-D-aspartate receptor functional activity: an in vivo microdialysis study
    Luca Ferraro
    Department of Clinical and Experimental Medicine, Pharmacology Section and LTTA Centre, Universityof Ferrara, Ferrara, Italy
    J Neurosci Res 89:1618-26. 2011
    The aim of the present in vivo microdialysis study was to investigate whether the tridecapeptide neurotensin (NT) influences the N-methyl-D-aspartate (NMDA) receptor-mediated increase of cortical glutamate transmission in freely moving ..
  101. pmc Interaction between brain chemistry and physiology after traumatic brain injury: impact of autoregulation and microdialysis catheter location
    Ivan Timofeev
    Division of Neurosurgery, University of Cambridge, Addenbrooke s Hospital, Cambridge, United Kingdom
    J Neurotrauma 28:849-60. 2011
    Bedside monitoring of cerebral metabolism in traumatic brain injury (TBI) with microdialysis is gaining wider clinical acceptance...

Research Grants76

  1. Hypothalamic Orexin Role in Menopause-Associated Hot Flashes and Mood/Sleep Disru
    PHILIP LEE JOHNSON; Fiscal Year: 2013
    ..focused on whole animal physiology and behavior, combined with in vivo neurochemistry measures using microdialysis;stereotaxic delivery of pharmacological and tract tracing compounds, and ex vivo functional brain imaging ..
  2. Tyrosine Availability Influences Mesocortico-Limbic Catecholamines
    GEORGE EUGENE JASKIW; Fiscal Year: 2012
    ..We will use in vivo microdialysis to sample the fluid between brain cells in awake animals and measure dopamine as well as noradrenaline and ..
  3. CRF-Oxytocin Interaction in the Regulation of Stress and Affect
    Joanna Dabrowska; Fiscal Year: 2013
    ..the role of CRFR2 in the CRF-OT relationship using integrative neuroanatomical, neurophysiological, in vivo microdialysis, and behavioral approaches...
  4. 5-HT transporter function: Interaction of hormones and antidepressants
    Alan Frazer; Fiscal Year: 2013
    ..Experiments will be carried out in ovariectomized female rats. The new techniques to be used are in vivo microdialysis and the forced swimming test (FST)...
  5. The Kallikrein/Kinin-Mast Cell Interaction in Volume Overload
    CHIH CHANG KEVIN WEI; Fiscal Year: 2013
    ..Using in vivo microdialysis, we have shown that interstitial infusion of BK in the normal rat is associated with increase in MCs and ECM ..
  6. Mechanisms of Vasovagal Syncope
    Julian M Stewart; Fiscal Year: 2013
    ..Our approach is two-fold: 1) We will use intradermal microdialysis and laser Doppler flowmetry (LDF) to delineate the microvascular mechanisms of NO modulation of noradrenergic ..
  7. Pathogenesis of Postoperative Cognitive Dysfunction
    Zhongcong Xie; Fiscal Year: 2013
    ..with higher baseline A[unreadable] levels), and employ Western blot, ELISA, immunohistochemistry, RT-PCR, microdialysis, RNAi, the Fear Conditioning Test, and an intra-dimensional/extra-dimensional digging task...
  8. RYGB Improves Metabolism by Interrupting the Gastric Adipose Tissue Axis
    Naji N Abumrad; Fiscal Year: 2013
    ..approaches: (i) In vivo studies to determine insulin sensitivity in liver and skeletal muscle and microdialysis of subcutaneous adipose tissue to assess tissue-specific oxidative stress, cytokine production and lipolysis...
  9. HYPOXIA AND CONTROL OF FETAL BREATHING MOVEMENTS
    Brian J Koos; Fiscal Year: 2010
    ..Inhibitors of ATP degradation and the synaptic release of ATP will be administered locally to Pf via microdialysis while measuring in real time extracellular levels of ADO and ATP in Pf...
  10. Vascular effects of dietary salt in humans with salt-resistant BP
    William B Farquhar; Fiscal Year: 2013
    ..dilation and cutaneous vasodilation in response to local heating using laser Doppler flowmetry and intradermal microdialysis will be assessed in the forearm to evaluate the microvasculature...
  11. CHOLINERGIC MECHANISMS OF REM SLEEP GENERATION
    Helen A Baghdoyan; Fiscal Year: 2013
    ..Methods include: 1) in vivo microdialysis and microinjection for drug delivery to selective brain regions and for collecting endogenously released ..
  12. Novel therapy for monoamine neurotransmitter deficiency in PKU
    Cary O Harding; Fiscal Year: 2013
    ..In the final aim of the project, we will investigate, using microdialysis methods, whether acute administration of (6R)-5,6,7,8-tetrahydrobiopterin (BH4), the required cofactor for TH ..
  13. Microdialysis Studies of Seizure-Induced Oxidative Stress
    Craig E Lunte; Fiscal Year: 2013
    ..b>Microdialysis sampling in discrete brain regions along with simultaneous recording of electrocorticographic (ECoG) activity ..
  14. Dopaminergic Mechanisms of Cytokine-Induced Behavioral Change
    Andrew H Miller; Fiscal Year: 2012
    ..To test these hypotheses, in vivo microdialysis of extracellular DA as well as PET neuroimaging of DAT binding and FDOPA uptake will be conducted in animals ..
  15. Transscleral Transport & Polymeric Delivery of Antibody & Steroids for Macular Ed
    Ashim K Mitra; Fiscal Year: 2010
    ..suspended in a thermosensitive PLGA-PEG-PLGA gel, following subconjunctival administration, using ocular microdialysis technique in anesthetized and conscious rabbit model...
  16. Sex and Stress Mechanisms of Vulnerability to Addiction
    Rajita Sinha; Fiscal Year: 2011
    ..and molecular neuroadaptations within the nucleus accumbens, prefrontal cortex and amygdala using in vivo microdialysis, Western blot analysis of downstream molecular markers (e.g., GluR1, AFosB, CDK5) and proteomics...
  17. Local Vasoconstriction in Postural Tachycardia Syndrome
    Julian M Stewart; Fiscal Year: 2013
    ..NO deficiency in LFP caused by Ang-II/oxidase induced oxidative stress? Experiments use intradermal microdialysis probes, laser Doppler flowmetry, and the NO-dependent local heating response to measure Ang-II and Ang-(1-7) ..
  18. Adult Neurogenesis: Regulation By Sleep
    DENNIS J MCGINTY; Fiscal Year: 2013
    ..DG GABA release will be measured by in vivo microdialysis. We will examine changes in GABA release during specific sleep events, including NREM sleep-associated sharp ..
  19. Ecstasy and the Dorsomedial Hypothalamus
    Daniel E Rusyniak; Fiscal Year: 2010
    ..for the markers of neuroendocrine activation, HPLC analysis of serum and brain catecholamines, microdialysis and C-fos immunohistochemistry...
  20. Mechanisms of Cardiac Afferents and CNS Reflex Processing
    John C Longhurst; Fiscal Year: 2013
    ..We will use combined whole animal reflex, cellular electrophysiological, microdialysis/HPLC measurement and pharmacological approaches in the proposed investigations...
  21. Mechanisms of regulation of ethanol intake by lateral habenula
    Jiang Hong Ye; Fiscal Year: 2013
    ..of glutamate receptors using Western blotting, and extracellular levels of glutamate in the LHb and RMTg using microdialysis techniques...
  22. 5-HT transporter function: Interaction of antidepressants and hormones
    Alan Frazer; Fiscal Year: 2013
    ..principal goal of this proposal is to extend these studies using other measures, either neurochemical (in vivo microdialysis) or behavioral (forced swimming test (FST)), to see if additional types of evidence can be obtained showing ..
  23. Neurobiology of Social Support
    CAROL SUE CARTER; Fiscal Year: 2010
    ..Measures will include behavioral and neuroendocrine responses (measured by brain microdialysis, cFos and immunocytochemistry, and blood levels of OT, AVP and corticosterone)...
  24. Cytokine Effects on Amphetamine-Induced Dopamine Release using PET Neuroimaging
    Jennifer C Felger; Fiscal Year: 2011
    ..Preliminary findings from in vivo microdialysis indicate decreased basal ganglia DA under resting conditions and following amphetamine (AMPH) administration ..
  25. ApoE receptor pathways and abeta metabolism
    Guojun Bu; Fiscal Year: 2013
    ..In Aim 2, we will use in vivo microdialysis technique to assess the roles of apoE isoforms and apoE receptors in brain A[unreadable] clearance...
  26. Unihemispheric sleep: implications for mechanisms of sleep control and function i
    Jerome M Siegel; Fiscal Year: 2013
    ..In pursuance of these objectives, we will use bilateral in vivo microdialysis to monitor the release of key neurotransmitters during these states, Fos immunohistochemistry to identify cell ..
  27. Negative symptoms of schizophrenia: animal models
    Athina Markou; Fiscal Year: 2013
    ..PCP-induced cognitive deficits and their potential reversal by these atypical antipsychotics by using in vivo microdialysis to measure monoamine, glutamate, and GABA levels in the prefrontal cortex and the nucleus accumbens...
  28. CNS circuitry and receptors mediating the effects of MDMA
    Daniel E Rusyniak; Fiscal Year: 2013
    ..to determine the effects of inhibiting or exciting the regions of interest on MDMA-evoked responses;and (3) microdialysis techniques to determine how inhibiting or exciting the regions of interest affect GABAergic and glutamatergic ..
  29. GCs, CRF and Stressor-Induced Relapse
    John R Mantsch; Fiscal Year: 2013
    ..stressor-induced regulation of NA DA and its involvement in stressor-induced cocaine seeking using in vivo microdialysis in and administration of DA receptor antagonists into the NA core and shell...
  30. Impact of Corticotropin Releasing Factor (CRF) on Sleep Regulation
    Ronald Szymusiak; Fiscal Year: 2013
    ..CRF agonists will be delivered directly into the MnPN and VLPO via a microdialysis probe, while single unit activity is recorded immediately adjacent to the probe...
  31. Adolescent Binge Drinking Increases Adult Alcohol Intake: Glutamate Mechanisms
    Deborah A Finn; Fiscal Year: 2013
    ..Consistent with the inverse relationship between extracellular glutamate levels (via microdialysis) and nerve terminal glutamate immunolabelling, repeated bouts of binge drinking significantly increased ..
  32. Modeling Individual Differences in PTSD
    Marlene A Wilson; Fiscal Year: 2013
    ..Aim 2 uses microdialysis to assess individual differences in amygdalar responses in NPY, CORT and NE during extinction of fear-..
  33. Serotonergic Contribution to Adolescent Risk Taking
    ANDREW EMMETT ARRANT; Fiscal Year: 2012
    ..Preliminary microdialysis in the prefrontal cortex showed that adolescent rats have a lower serotonergic response to fenfluramine (1, 2...
  34. Gustatory Control of Sodium Appetite
    Ralph Norgren; Fiscal Year: 2012
    ..Specifically, we will use (1) central lesions, (2) microdialysis, and (3) FOS immunohistochemistry to determine if parabrachial gustatory neural activity distributed via the ..
  35. Mechanisms and Consequences of Locus Coeruleus Activation
    KENICHIRO HAYASHIDA; Fiscal Year: 2013
    ..by which GBP modulates GABA regulation of pre-synaptic glutamate release in the LC using synaptosomes and microdialysis. Secondly, we will examine mechanisms by which GBP modulates the unique astroglial-neuronal interaction in the ..
  36. NEURAL STEROIDOGENIC ENZYMES AND BRAIN FUNCTION
    BARNETT ALAN SCHLINGER; Fiscal Year: 2013
    ..this gap we have developed the ability to measure steroid levels in freely-behaving songbirds using in vivo microdialysis. Our findings show that E2 and testosterone (T) levels in the songbird brain fluctuate relatively rapidly ..
  37. Optogenetic Control of Vigilance State Transition
    Luis de Lecea; Fiscal Year: 2013
    ..We will monitor photostimulation-induced changes in Hcrt release by microdialysis. We will also monitor locomotor activity, anxiety, feeding, behavior and sleep/wake parameters...
  38. In Vivo Brain Dialysis of Neuropeptides and Neuroimmune Signaling Proteins
    Julie A Stenken; Fiscal Year: 2012
    ..In particular, microdialysis sampling of these important cytokines and peptides has been difficult to implement...
  39. Somatic cell gene transfer/neurological &clinical applications
    En Ju Lin; Fiscal Year: 2013
    ..In brief, in 1992 we demonstrated using human microdialysis that adenosine was released in the conscious human brain, and that during an ictal event rose to ..
  40. Gene-environment interaction: the brain CRF system in alcohol preferring msP rats
    Marisa Roberto; Fiscal Year: 2013
    ..Using electrophysiology together with in situ hybridization, autoradiography and brain microdialysis technique, we expect to obtain information at neurocircuitry and at functional levels on the significance of ..
  41. Influence of Endocrine Disruptors on Reproductive Neuroendocrine Function
    JOSEPH RAYMOND KURIAN; Fiscal Year: 2013
    ..However, to better support this hypothesis, using an in vivo microdialysis approach, they propose to address the following aims: 1) determine the dynamics of GnRH release during BPA ..
  42. Neurovascular Responses to Reflex Cooling in Essential Hypertensive Humans
    JODY GREANEY; Fiscal Year: 2013
    ..Intradermal microdialysis will be used to assess specific downstream cellular mechanisms (adrenergic- and Rho kinase-mediated pathways) ..
  43. Nicotine and the roles of nicotinic receptors in a rodent model of schizophrenia
    Russell W Brown; Fiscal Year: 2013
    ..in a sensitized dopamine response to nicotine in the nucleus accumbens core of adolescent rats as analyzed by microdialysis;3) we have reported enhanced behavioral sensitization and place conditioning to nicotine in rats neonatally ..
  44. Targeted Library Synthesis and Screening: Allosteric Modulators of Transporters
    Subramaniam Ananthan; Fiscal Year: 2013
    ..Envisioned in vivo studies with candidate molecules include (i) microdialysis experiments in rats (ii) locomotor studies in rats and (iii) determination of the effects of the compounds ..
  45. Effects of dietary obesity on midbrain dopamine systems
    EMMANUEL POTHOS; Fiscal Year: 2009
    ..pathways of the adult rat midbrain dopamine system (mesoaccumbens, nigrostriatal, corticolimbic) with in vivo microdialysis. Correlate changes in dopamine release with changes in behavior (locomotion, diet consumption, response to ..
  46. In Vivo Neuronal Modulation via Engineered G Protein-Coupled Receptors
    SARAH CLAIRE ROGAN; Fiscal Year: 2012
    ..To achieve these aims, a variety of techniques will be used, including immunohistochemistry, in vivo microdialysis, measurement of inositol phosphate accumulation, immunoblotting, and stereotaxic viral infusion...
  47. Mechanisms of Hypertension-induced Renal Injury
    Allen W Cowley; Fiscal Year: 2010
    ..be assessed at three time points during the development of hypertension (days 3, 7 and 14) using techniques of microdialysis to collect interstitial fluid for the determination of H2O2 and nitric oxide concentrations...
  48. Intra Cavitary Pharmacokinetics and Drug Resistance in Pulmonary Tuberculosis
    RUSSELL RYAN KEMPKER; Fiscal Year: 2013
    ..patient cohort of MDR-TB patients undergoing adjunctive surgery in the Republic of Georgia and an innovative microdialysis method we will assess SLD concentrations among various compartments including pulmonary cavities, the site of ..
  49. (Pro)renin receptor and renovascular hypertension
    Helmy M Siragy; Fiscal Year: 2013
    ..cellular and molecular techniques, siRNA and shRNA, Laser Scanning Confocal FRET microscopy and in vivo microdialysis to more rigorously test the proposed ideas...
  50. Neural Substrates of Phasic versus Sustained Fear
    David L Walker; Fiscal Year: 2013
    ..of selected brain regions, and the measurement of extracellular glutamate in different brain areas using microdialysis and high-pressure liquid chromatography (HPLC)...
  51. Brain Norepinephrine and Stress Reactivity
    David A Morilak; Fiscal Year: 2012
    ..These studies will employ microdialysis, behavioral pharmacology, and anatomical approaches...
  52. Locus Coeruleus Function and Methylphenidate Action
    BARRY DALE WATERHOUSE; Fiscal Year: 2013
    ..analysis of the distribution and density of noradrenergic profiles across various brain regions, 3) microdialysis and high pressure liquid chromatography with electrochemical detection (HPLC-EC) of NE release in sensory and ..
  53. Stressor controllability and anxiety: role of serotonin and prefrontal cortex.
    JOHN PAUL CHRISTIANSON; Fiscal Year: 2010
    ..selected brain nuclei, including: microinjection of excitatory or inhibitory compounds during stress, in vivo microdialysis, functional anatomy using retrograde tracing and immunohistochemistry for cellular activity markers...
  54. Modulating and Monitoring the Foreign Body Response to Implants
    Julie A Stenken; Fiscal Year: 2013
    ..EXPERIMENTAL APPROACH. Microdialysis sampling probes will be used as glucose sensor mimics that can concomitantly deliver mediators aimed to alter ..
  55. Social Influences on drug reward and monoamines
    Sondra T Bland; Fiscal Year: 2010
    ..accumbens and medial prefrontal cortex of isolation- or group-reared male and female rats using in vivo microdialysis. Understanding how drug and social rewards interact and how this may be mediated by the prior social ..
  56. Purinergic Mechanisms in Homeostatic Sleep Control
    Radhika Basheer; Fiscal Year: 2013
    ..Receptor specificity will be tested by reverse microdialysis during SD of antagonists of the P2 and, separately, to the A1 receptor...
  57. Continuous monitoring of anti-fibrinolytic therapy in cardiovascular surgery
    Francis G Spinale; Fiscal Year: 2012
    ..Through the use of microdialysis and microfluidics, the specific aims of this development project are (1) to complete calibration studies of a ..
  58. Oxidative Stress and Pathological Glutamate Release in Stroke
    Alexander A Mongin; Fiscal Year: 2013
    ..stress and pathological glutamate release in vivo in a rat model of transient focal ischemia, using a microdialysis approach and the intra-dialysate drug delivery;(AIM 3) to test the neuroprotective potential of the ..
  59. Essential Hypertension and Human Skin Blood Flow
    Lacy M Alexander; Fiscal Year: 2013
    ..by pairing state-of-the-art nitric oxide (NO) specific in vivo methodologies (local heating and intradermal microdialysis) with in vitro analysis of human skin samples to examine the precise signaling mechanisms underlying impaired ..
  60. Role of Habenula in Tobacco Addiction
    Ramiro Salas; Fiscal Year: 2013
    ..in which I worked on mice with mutations on nicotinic receptors (nAChR), using behavior, in vivo pharmacology, microdialysis, etc. My current goal is to verify that the conclusions of my mouse research are applicable to humans...
  61. Neuropeptides, Social Stress and Drugs of Abuse
    Klaus A Miczek; Fiscal Year: 2013
    ..discrete intracerebral microinjections to stimulate and block CRF-R1 in discrete neural regions, in vivo microdialysis for sampling extraneuronal fluid, high performance liquid chromatography for assaying these samples to ..
  62. Exercise, histamine receptors, and vascular function
    Steven A Romero; Fiscal Year: 2012
    ..agonist will be attempted by using single leg dynamic knee extension exercise along with the placement of microdialysis fibers to remove and infuse molecules and drugs of interest in the active skeletal muscle...
  63. Restoration of Estradiol Effects on Learning by Cholinergic Enhancement
    Robert Gibbs; Fiscal Year: 2009
    ..used in the treatment of Alzheimer's disease), with and without estradiol, and then studied using in vivo microdialysis and behavioral training...
  64. Rehabilitation of Stress-Induced Insomnia
    Pingfu Feng; Fiscal Year: 2009
    ..neonatal maternal deprivation, (2) polysomnographic recording, a classic technique for sleep evaluation, (3) microdialysis for extracellular fluid collection and (4) use radioimmunoassay to quantify the levels of CRH and orexins...
  65. DRUGS OF ABUSE, DOPAMINE & CONTROL BY BRAIN PEPTIDES
    BARTLEY HOEBEL; Fiscal Year: 1991
    Our progress in the three years of this grant shows that microdialysis can be used in behaving animals to monitor dopamine (DA), serotonin (SER), and their metabolites during body weight changes, self-stimulation or injection of drugs...
  66. CONTROL OF ENDOCANNABINOID RELEASE IN VIVO
    Daniele Piomelli; Fiscal Year: 2003
    ..We propose to use microdialysis and gas chromatography/mass spectrometry techniques to test the hypothesis that endogenous cannabinoid ..
  67. 5 HYDROXTRYPTAMINE MECHANISMS OF HALLUCINOGENIC DRUGS
    DAVID MOKLER; Fiscal Year: 1992
    ..The method of in vivo microdialysis for simultaneous measurement of the extracellular levels of 5-HT and its metabolite 5-hydroxyindoleacetic acid ..
  68. OREXIN AND THE CONTROL OF SLEEP AND WAKEFULNESS
    ROBERT MC CARLEY; Fiscal Year: 2005
    ..The key techniques to be used are novel combinations of multi-disciplinary methods including the use of microdialysis perfusion of antisense against mRNA of orexin receptors combined with electrographic recording of behavioral ..
  69. OXIDATION OF MACROMOLECULES IN SPINAL CORD INJURY
    Danxia Liu; Fiscal Year: 2001
    ..and an iron chelator (desferrioxamine) into the intrathecal space of the rat spinal cord by a microdialysis or a microcannula loop...
  70. NUCLEUS ACCUMBENS INVOLVEMENT IN COCAINE REINFORCEMENT
    GREGORY MARK; Fiscal Year: 2002
    ..b>Microdialysis will be used to measure ACh at four time points: 1) during initial exposure to cocaine, 2) after 2 weeks of ..
  71. ELECTROPHYSIOLOGY & PHARMACOLOGY OF SLEEP-WAKEFULNESS
    Mahesh Thakkar; Fiscal Year: 2004
    ..The key technique to be used is a novel combination of microdialysis and extracellular single unit recording in freely behaving cats. The nonREM sleep phase...
  72. DEPRESSION--LOCUS COERULEUS AFFECTS DOPAMINE VIA GALANIN
    JAY WEISS; Fiscal Year: 2001
    ..in VTA can reverse behavioral depression, and (2) commensurate changes in extracellular DA (measured by microdialysis) in forebrain also occur...
  73. IN VIVO INVESTIGATIONS USING MICRODIALYSIS SAMPLING
    Craig E Lunte; Fiscal Year: 2010
    ..The objective of this proposal is to develop in vivo microdialysis sampling in the colonic mucosa to be used in conjunction with microanalytical techniques to monitor the ..
  74. Chemotherapy dose monitoring using a wearable, microdialysis-based assay system
    GEORGE SUBREBOST; Fiscal Year: 2010
    ..This system will couple microdialysis blood sampling to a miniaturized immunoassay for 5-FU and also with an embedded control system (small computer)..
  75. Neurochemistry of Cocaine Reinforcement
    NICHOLAS GOEDERS; Fiscal Year: 2002
    ..experiment (self-administration and yoked infusions) with the complex neurochemical procedure, in vivo microdialysis from different brain areas thought to be involved in the mediation of cocaine-seeking behavior...
  76. ROLE OF DOPAMINE IN MDMA-INDUCED SEROTONIN DEPLETION
    J Nash; Fiscal Year: 1991
    ..The proposed studies will measure the acute release of DA, 5-HT and their metabolites using in vivo microdialysis following the systemic or local administration of MDMA...